FastQCFastQC Report
Fri 15 Nov 2024
HBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118571
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT5320.44867632051682116No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA3150.2656636108323283No Hit
CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT2870.24204906764723247No Hit
CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA2410.20325374670028926No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT2200.1855428393114674No Hit
CTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAG2190.18469946276914254No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG2140.1804825800575183No Hit
CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG2130.1796392035151934No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT2100.17710907388821887No Hit
CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT1870.1577114134147473No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA1830.15433790724544788No Hit
CTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTT1800.15180777761847333No Hit
CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT1780.15012102453382362No Hit
CGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGA1680.1416872591105751No Hit
CTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTC1650.13915712948360054No Hit
GCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG1630.13747037639895085No Hit
AAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGG1610.13578362331430113No Hit
CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC1590.13409687022965142No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT1490.12566310480640291No Hit
CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA1460.12313297517942837No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC1440.12144622209477865No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT1410.1189160924678041No Hit
GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC1380.11638596284082954No Hit
CTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAG1370.1155425862985047No Hit
GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC1310.11048232704455559No Hit
GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCT1290.10879557395990587No Hit
TCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACC1270.10710882087525617No Hit
CTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGAC1250.10542206779060646No Hit
GTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCG1240.10457869124828162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)