FastQCFastQC Report
Fri 15 Nov 2024
HBR_Rep2_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBR_Rep2_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144826
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT5650.390123320398271No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC4210.29069365997818075No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA4140.2858602737077596No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT4040.2789554361785867No Hit
CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA3840.26514576112024085No Hit
GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT3470.23959786226230095No Hit
CGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAAT3440.2375264110035491No Hit
CCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAA3410.23545495974479722No Hit
CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC2920.2016212558518498No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG2490.17193045447640615No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG2380.16433513319431592No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT2340.16157319818264676No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA2240.15466836065347384No Hit
GGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAA2230.15397787690055653No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA2220.15328739314763926No Hit
GCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCC2210.15259690939472195No Hit
GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG2190.15121594188888735No Hit
GGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATA2180.15052545813597004No Hit
GTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCT2140.14776352312430088No Hit
CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA2070.1429301368538798No Hit
CTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGC2030.14016820184221065No Hit
CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT2030.14016820184221065No Hit
CCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTT1970.1360252993247069No Hit
CCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGAT1930.13326336431303773No Hit
CGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGAT1930.13326336431303773No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG1900.13119191305428585No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT1900.13119191305428585No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA1890.13050142930136854No Hit
CGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTA1850.12773949428969936No Hit
CTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTG1830.12635852678386478No Hit
GTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCAC1810.12497755927803018No Hit
ATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTG1790.1235965917721956No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG1780.1229061080192783No Hit
CGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTT1760.12152514051344372No Hit
GGGGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAA1700.11738223799593996No Hit
GGATAATTGCGCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAG1690.11669175424302265No Hit
CGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCT1600.11047740046676702No Hit
CCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGT1580.10909643296093242No Hit
CTGTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAACGA1580.10909643296093242No Hit
CCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTA1580.10909643296093242No Hit
GGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAG1570.10840594920801515No Hit
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT1550.10702498170218055No Hit
ATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATC1540.10633449794926325No Hit
CTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGC1540.10633449794926325No Hit
GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG1520.10495353044342867No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA1520.10495353044342867No Hit
GTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCC1510.10426304669051137No Hit
CGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAAT1500.10357256293759408No Hit
CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT1480.1021915954317595No Hit
CCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGT1470.1015011116788422No Hit
GTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACC1460.1008106279259249No Hit
GGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGG1460.1008106279259249No Hit
GCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGA1450.1001201441730076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)