FastQCFastQC Report
Fri 15 Nov 2024
UHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162373
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT8480.5222543156805626No Hit
CGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAAT5570.3430373276345205No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT5390.3319517407450746No Hit
CCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAA5230.3220978857322338No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT4650.28637766131068587No Hit
CTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATAT3680.2266386652953385No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT3100.1909184408737906No Hit
CTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTG2990.18414391555246254No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA2990.18414391555246254No Hit
CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA2670.16443620552678093No Hit
CTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTT2620.16135687583526817No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG2610.16074100989696563No Hit
CTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAA2580.15889341208205798No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC2510.15458235051394012No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA2480.15273475269903247No Hit
CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC2340.14411262956279677No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT2330.14349676362449423No Hit
CAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTT2330.14349676362449423No Hit
CTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTT2310.14226503174788913No Hit
CCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCT2290.14103329987128402No Hit
CTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAA2220.13672223830316618No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG2170.1336429086116534No Hit
CTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTC2100.12933184704353556No Hit
CGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTT2070.12748424922862792No Hit
CAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAGGAGAT2060.12686838329032535No Hit
CTGTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAACGA2050.1262525173520228No Hit
CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA2030.1250207854754177No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTAT2020.12440491953711516No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA2010.1237890535988126No Hit
CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA1960.12070972390729987No Hit
CTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGG1960.12070972390729987No Hit
GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT1900.11701452827748457No Hit
CCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCC1890.116398662339182No Hit
CTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTC1860.11455106452427435No Hit
CTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGCA1830.1127034667093667No Hit
CGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTT1830.1127034667093667No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA1820.11208760077106417No Hit
CCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGC1760.10839240514124886No Hit
ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT1750.1077765392029463No Hit
GTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAG1720.10592894138803866No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT1700.10469720951143355No Hit
AGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAG1670.1028496116965259No Hit
CCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGAC1660.10223374575822335No Hit
CCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCA1660.10223374575822335No Hit
CTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCC1640.10100201388161824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)