FastQCFastQC Report
Fri 15 Nov 2024
UHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162373
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT6820.42002056992233927No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT4090.2518891687657431No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA3580.22048000591231304No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG3160.19461363650360589No Hit
TGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAA2890.17798525616943706No Hit
CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT2870.17675352429283195No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC2650.16320447365017582No Hit
CAAAAACGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGT2430.14965542300751972No Hit
CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG2420.14903955706921718No Hit
CAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCGCC2340.14411262956279677No Hit
CGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATC2310.14226503174788913No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT2260.13918570205637637No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA2250.13856983611807383No Hit
CTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAAC2250.13856983611807383No Hit
CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA2240.1379539701797713No Hit
CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT2070.12748424922862792No Hit
CTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCT2020.12440491953711516No Hit
CTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGTT1970.1213255898456024No Hit
CAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATT1880.11578279640087946No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT1850.11393519858597181No Hit
TATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATT1840.11331933264766925No Hit
CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT1840.11331933264766925No Hit
CGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCG1840.11331933264766925No Hit
GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA1840.11331933264766925No Hit
GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCT1730.1065448073263412No Hit
CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC1670.1028496116965259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)