FastQCFastQC Report
Fri 15 Nov 2024
UHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185442
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT9860.5317026347860787No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT5060.2728615955393061No Hit
CGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAAT4860.26207655223735726No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT4680.25237001326560327No Hit
CCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAA4540.24482048295423908No Hit
CTCAATTTGAAGTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATAT4000.21570086603897712No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA3960.2135438573785874No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG3570.19251302293978712No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTT3380.1822672318029357No Hit
CTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTG3060.1650111625198175No Hit
CCGGGATCGGGCAAAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCA2980.160697145199038No Hit
GGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTG2960.1596186408688431No Hit
CTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTT2840.15314761488767376No Hit
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT2760.14883359756689424No Hit
CTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTT2740.14775509323669936No Hit
CAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTT2700.14559808457630957No Hit
CTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAA2700.14559808457630957No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC2690.14505883241121212No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA2690.14505883241121212No Hit
CTCAAATTTCGCACTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAA2640.1423625715857249No Hit
CTGTGATCTACTAGCGCCAATCTATATTACTCACAATATCCTAAAAACGA2550.13750930209984794No Hit
CCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCC2480.13373453694416582No Hit
CCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCT2470.1331952847790684No Hit
CTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAA2330.12564575446770418No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTAT2250.12133173714692465No Hit
CGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTT2250.12133173714692465No Hit
CTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTC2200.11863547632143742No Hit
CTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCC2150.11593921549595022No Hit
ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT2150.11593921549595022No Hit
CAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGATGAGGAGAT2130.11486071116575533No Hit
GCCTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAG2130.11486071116575533No Hit
CTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTC2090.11270370250536556No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA2070.11162519817517068No Hit
GTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGT2040.11000744167987833No Hit
GGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTT2030.1094681895147809No Hit
GTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCA2020.10892893734968347No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA2000.10785043301948856No Hit
CTCACTTAGTTACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTT1940.10461492002890392No Hit
CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA1930.10407566786380647No Hit
CTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCC1920.10353641569870901No Hit
CTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGG1900.10245791136851415No Hit
GGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCC1880.10137940703831924No Hit
GTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTG1870.10084015487322182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)