FastQCFastQC Report
Fri 15 Nov 2024
UHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185442
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT7060.38071202855879466No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT4110.22163263985504902No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA3750.20221956191154108No Hit
CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG3310.17849246664725357No Hit
CGAATAGCTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAG3270.17633545798686381No Hit
CCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGT3060.1650111625198175No Hit
TGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAA2980.160697145199038No Hit
CAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATT2850.1536868670527712No Hit
CAAAAACGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGT2840.15314761488767376No Hit
CTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCT2720.14667658890650445No Hit
CAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATTCGCC2700.14559808457630957No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA2660.1434410759159198No Hit
CGAAACTGGAAACCGACCGCCGCCATATCAGAAATCGCATTCATGAAATC2650.14290182375082236No Hit
CTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCAC2640.1423625715857249No Hit
CCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTAT2490.13427378910926327No Hit
GGCCGATTGTCGCAGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCT2180.11755697199124254No Hit
CTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGTT2170.1170177198261451No Hit
GGAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCA2140.11539996333085277No Hit
CGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATG2120.11432145900065788No Hit
TATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATT2090.11270370250536556No Hit
CTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAAC2050.11054669384497579No Hit
CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT1990.10731118085439113No Hit
GAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCC1990.10731118085439113No Hit
CTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTT1910.10299716353361159No Hit
CTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGA1890.1019186592034167No Hit
CCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGC1890.1019186592034167No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC1870.10084015487322182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)