Sample median_sequence_length avg_sequence_length Total Sequences sequence_length_distribution Sequences flagged as poor quality per_base_sequence_quality per_tile_sequence_quality Encoding per_sequence_quality_scores Filename per_base_sequence_content basic_statistics %GC per_sequence_gc_content total_deduplicated_percentage File type sequence_duplication_levels overrepresented_sequences per_base_n_content adapter_content Sequence length HBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1 100 100.0 118571.0 pass 0.0 pass pass Sanger / Illumina 1.9 pass HBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz fail pass 50.0 warn 57.31418306331227 Conventional base calls warn warn pass pass 100.0 HBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2 100 100.0 118571.0 pass 0.0 pass pass Sanger / Illumina 1.9 pass HBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2.fastq.gz fail pass 50.0 warn 56.44044496546373 Conventional base calls warn warn pass pass 100.0 HBR_Rep2_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1 100 100.0 144826.0 pass 0.0 pass warn Sanger / Illumina 1.9 pass HBR_Rep2_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz fail pass 50.0 warn 55.650919033875134 Conventional base calls warn warn pass pass 100.0 HBR_Rep2_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2 100 100.0 144826.0 pass 0.0 pass pass Sanger / Illumina 1.9 pass HBR_Rep2_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2.fastq.gz warn pass 50.0 warn 54.805076436551445 Conventional base calls warn warn pass pass 100.0 HBR_Rep3_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1 100 100.0 129786.0 pass 0.0 pass pass Sanger / Illumina 1.9 pass HBR_Rep3_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz fail pass 50.0 warn 56.670981461790944 Conventional base calls warn warn pass pass 100.0 HBR_Rep3_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2 100 100.0 129786.0 pass 0.0 pass pass Sanger / Illumina 1.9 pass HBR_Rep3_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2.fastq.gz warn pass 50.0 warn 55.94208928543911 Conventional base calls warn warn pass pass 100.0 UHR_Rep1_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1 100 100.0 227392.0 pass 0.0 pass pass Sanger / Illumina 1.9 pass UHR_Rep1_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz fail pass 49.0 warn 55.46553209338351 Conventional base calls warn warn pass pass 100.0 UHR_Rep1_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2 100 100.0 227392.0 pass 0.0 pass pass Sanger / Illumina 1.9 pass UHR_Rep1_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz warn pass 49.0 warn 54.74198232216694 Conventional base calls warn warn pass pass 100.0 UHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1 100 100.0 162373.0 pass 0.0 pass pass Sanger / Illumina 1.9 pass UHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz fail pass 48.0 warn 53.220670924353186 Conventional base calls warn warn pass pass 100.0 UHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2 100 100.0 162373.0 pass 0.0 pass pass Sanger / Illumina 1.9 pass UHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz fail pass 49.0 warn 52.595566996976096 Conventional base calls warn warn pass pass 100.0 UHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1 100 100.0 185442.0 pass 0.0 pass pass Sanger / Illumina 1.9 pass UHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz fail pass 49.0 warn 48.537548128255736 Conventional base calls fail warn pass pass 100.0 UHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2 100 100.0 185442.0 pass 0.0 pass pass Sanger / Illumina 1.9 pass UHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz warn pass 49.0 warn 47.28001207924849 Conventional base calls fail warn pass pass 100.0