FastQCFastQC Report
Fri 15 Nov 2024
HBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117179
Sequences flagged as poor quality0
Sequence length46-87
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATA4640.3959753880814822No Hit
TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT4060.3464784645712969No Hit
GTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGA4030.34391827887249427No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG3920.33453093131021766No Hit
TGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTT3800.32429018851500696No Hit
GCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTT3420.2918611696635063No Hit
GATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCA3220.2747932650048217No Hit
TATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAA2730.23297689859104448No Hit
ACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAAC2480.21164201776768873No Hit
CTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCG2300.19628090357487263No Hit
ATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGG2240.19116053217726725No Hit
ACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAAT2150.1834799750808592No Hit
GGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGCCCTAACTGCGC1990.16982565135391153No Hit
ACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGA1910.16299848949043771No Hit
AAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCATGTGTAGTGCGCC1870.15958490855870078No Hit
AGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCT1870.15958490855870078No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG1840.1570247228598981No Hit
TTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAA1790.15275774669522696No Hit
ACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGA1710.14593058483175314No Hit
TCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGCCCTAACTGCGCAG1670.1425170039000162No Hit
GTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAA1630.1391034229682793No Hit
CATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCT1630.1391034229682793No Hit
CAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATA1610.13739663250241085No Hit
ATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATA1610.13739663250241085No Hit
ATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTA1600.13654323726947662No Hit
GGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAAC1550.13227626110480548No Hit
ACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCGCGGTTTT1550.13227626110480548No Hit
AGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACG1520.12971607540600277No Hit
GACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCGCGGTTT1500.12800928494013433No Hit
AAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAG1470.12544909924133163No Hit
GGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTAT1470.12544909924133163No Hit
TCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGATCGCAGGTTGTG1440.12288891354252894No Hit
AGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATAT1430.12203551830959472No Hit
ATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTC1420.12118212307666049No Hit
AACGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAG1390.11862193737785781No Hit
TTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAG1390.11862193737785781No Hit
TACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCG1390.11862193737785781No Hit
TACTCACAATATCCTAAAAACGATCAGCCGGCAGGTTCATTTTAAACGCG1380.11776854214492356No Hit
AGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCT1380.11776854214492356No Hit
GTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACA1360.11606175167905512No Hit
GAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGC1340.11435496121318667No Hit
AACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCA1330.11350156598025243No Hit
GACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATT1320.1126481707473182No Hit
GCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATC1310.11179477551438398No Hit
CAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCT1290.11008798504851551No Hit
GCGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGT1270.10838119458264706No Hit
GCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGATTGCC1250.10667440411677862No Hit
AGATTGCACCGTTGTTATAATCGACCCGGGCTGCGACAATCGGCCGTATG1240.10582100888384437No Hit
TTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATC1230.10496761365091013No Hit
AGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTA1220.10411421841797593No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT1210.10326082318504169No Hit
TTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAA1190.10155403271917324No Hit
CTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTT1180.100700637486239No Hit
ACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAG1180.100700637486239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)