FastQCFastQC Report
Fri 15 Nov 2024
HBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117179
Sequences flagged as poor quality0
Sequence length46-87
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTT5330.454859659153944No Hit
AAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATAT3160.2696728936072163No Hit
ACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATC2840.24236424615332097No Hit
CCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATC2350.20054787973954377No Hit
ATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCC2170.18518676554672764No Hit
TTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTACG2160.18433337031379343No Hit
AAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAG2140.18262657984792496No Hit
GACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT2120.18091978938205652No Hit
ACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGC2090.17835960368325382No Hit
ATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATA1840.1570247228598981No Hit
TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC1840.1570247228598981No Hit
CGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA1750.14934416576349005No Hit
GAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGAC1740.14849077053055582No Hit
CCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGC1670.1425170039000162No Hit
GGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAA1660.14166360866708197No Hit
TTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAA1640.13995681820121353No Hit
TACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTA1620.1382500277353451No Hit
TTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTA1590.13568984203654239No Hit
AGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGAT1470.12544909924133163No Hit
TCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGACGAATTGCCAG1470.12544909924133163No Hit
AACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCC1450.12374230877546318No Hit
ACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCT1400.11947533261079205No Hit
ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC1370.11691514691198936No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT1300.11094138028144974No Hit
AGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGT1280.10923458981558129No Hit
AAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGT1250.10667440411677862No Hit
ATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCT1250.10667440411677862No Hit
ATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCT1190.10155403271917324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)