FastQCFastQC Report
Fri 15 Nov 2024
HBR_Rep2_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBR_Rep2_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142968
Sequences flagged as poor quality0
Sequence length44-87
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATA5970.41757596105422196No Hit
GATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCA4460.3119579206535728No Hit
GTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGA4410.30846063454759104No Hit
TGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTT4080.2853785462481115No Hit
GCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTT3960.27698505959375525No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG3470.242711655755134No Hit
TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT3470.242711655755134No Hit
ACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAAC3360.23501762632197415No Hit
CTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCG2950.2063398802529237No Hit
TATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAA2740.19165127860780035No Hit
ACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAAT2550.17836159140506966No Hit
GGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGCCCTAACTGCGC2470.17276593363549886No Hit
ATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGG2420.1692686475295171No Hit
ATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTC2350.16437244698114264No Hit
ACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGA2330.16297353253874994No Hit
AAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAG2270.15877678921157182No Hit
ACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCGCGGTTTT2250.1573778747691791No Hit
GGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAAC2240.15667841754798276No Hit
GGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTAT2230.1559789603267864No Hit
ATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATA2220.15527950310559005No Hit
AGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATAT2130.1489843881148229No Hit
AGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCT2130.1489843881148229No Hit
TCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGCCCTAACTGCGCAG2110.1475854736724302No Hit
ACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGA2100.14688601645123384No Hit
AAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCATGTGTAGTGCGCC2090.14618655923003748No Hit
TACTCACAATATCCTAAAAACGATCAGCCGGCAGGTTCATTTTAAACGCG2000.13989144423927033No Hit
GTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAA1980.13849252979687762No Hit
GGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATA1950.13639415813328856No Hit
GACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCGCGGTTT1940.1356947009120922No Hit
TTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAA1910.13359632924850315No Hit
AGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACG1910.13359632924850315No Hit
TCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGATCGCAGGTTGTG1840.1287001287001287No Hit
ATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTA1810.12660175703653964No Hit
TTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAG1800.1259022998153433No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG1790.12520284259414693No Hit
AACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCA1760.12310447093055789No Hit
GTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACA1730.12100609926696884No Hit
CGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAA1700.11890772760337978No Hit
CATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCT1670.11680935593979072No Hit
GAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGC1660.11610989871859437No Hit
GTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGA1660.11610989871859437No Hit
GGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAA1610.11261261261261261No Hit
TATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAA1610.11261261261261261No Hit
AACGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAG1600.11191315539141626No Hit
CAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATA1600.11191315539141626No Hit
GCGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGT1580.11051424094902355No Hit
AGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCT1580.11051424094902355No Hit
TAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACT1570.10981478372782721No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT1560.10911532650663086No Hit
GCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGATTGCC1550.10841586928543451No Hit
GACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATT1550.10841586928543451No Hit
TACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCG1550.10841586928543451No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG1540.10771641206423815No Hit
TTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAA1530.1070169548430418No Hit
AGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTA1530.1070169548430418No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA1530.1070169548430418No Hit
GCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATC1480.10351966873706005No Hit
CAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTT1470.1028202115158637No Hit
AGATTGCACCGTTGTTATAATCGACCCGGGCTGCGACAATCGGCCGTATG1470.1028202115158637No Hit
TATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCT1440.10072183985227463No Hit
TAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCCAATTCGCGTCATC1430.10002238263107828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)