FastQCFastQC Report
Fri 15 Nov 2024
HBR_Rep3_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBR_Rep3_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128070
Sequences flagged as poor quality0
Sequence length48-87
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATA4680.365425158116655No Hit
TGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTT4350.3396579995315062No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG4220.32950730069493245No Hit
TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT4020.3138908409463575No Hit
GTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGA3950.3084250800343562No Hit
GCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTT3660.2857812133989225No Hit
GATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCA3650.2850003904114937No Hit
ACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAAC3070.2397126571406262No Hit
TATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAA2870.2240961973920512No Hit
CTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCG2770.21628796751776372No Hit
GGTCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGCCCTAACTGCGC2250.17568517217146873No Hit
ACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGA2140.1670961193097525No Hit
ATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTC2140.1670961193097525No Hit
ATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATA2140.1670961193097525No Hit
ACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAAT2090.16319200437260872No Hit
ATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGG1990.15538377449832122No Hit
TTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAA1970.15382212852346372No Hit
ACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGA1970.15382212852346372No Hit
AAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAG1960.15304130553603498No Hit
TCAAGGCGAAACCGCAGCAAACTTCCTCAGACGCTGCCCTAACTGCGCAG1900.14835636761146248No Hit
AGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATAT1890.14757554462403372No Hit
GGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAAC1870.14601389864917624No Hit
GTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAA1850.14445225267431872No Hit
AGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACG1840.14367142968688998No Hit
ATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTA1810.14132896072460374No Hit
GGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTAT1790.1397673147497462No Hit
TCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGATCGCAGGTTGTG1760.13742484578745998No Hit
AAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCATGTGTAGTGCGCC1750.1366440228000312No Hit
AACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCA1750.1366440228000312No Hit
GGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATA1710.13352073085031624No Hit
TTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAG1700.13273990786288747No Hit
AGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCT1690.13195908487545874No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG1670.13039743890060124No Hit
GCGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGT1650.12883579292574374No Hit
TACTCACAATATCCTAAAAACGATCAGCCGGCAGGTTCATTTTAAACGCG1640.128054969938315No Hit
ACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCGCGGTTTT1620.12649332396345747No Hit
AGCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCT1600.12493167798859998No Hit
GACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCGCGGTTT1550.12102756305145622No Hit
CATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCT1550.12102756305145622No Hit
GAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGC1520.11868509408916998No Hit
GCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATC1510.11790427110174125No Hit
CTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTT1460.11400015616459748No Hit
AACGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAG1460.11400015616459748No Hit
CAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATA1460.11400015616459748No Hit
AGATTGCACCGTTGTTATAATCGACCCGGGCTGCGACAATCGGCCGTATG1460.11400015616459748No Hit
TACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCG1450.11321933317716873No Hit
GTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGA1380.10775357226516749No Hit
CAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTT1370.10697274927773874No Hit
GGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAA1360.10619192629030998No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG1360.10619192629030998No Hit
GACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATT1350.10541110330288123No Hit
TTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAA1310.10228781135316624No Hit
TAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACT1310.10228781135316624No Hit
AATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTC1310.10228781135316624No Hit
CACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCGTCCCTATACGCGA1310.10228781135316624No Hit
TAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCCAATTCGCGTCATC1300.10150698836573749No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA1300.10150698836573749No Hit
AGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTA1290.10072616537830872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)