FastQCFastQC Report
Fri 15 Nov 2024
HBR_Rep3_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBR_Rep3_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128070
Sequences flagged as poor quality0
Sequence length48-87
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTT5490.4286718200983837No Hit
ACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATC2970.23190442726633873No Hit
AAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATAT2910.22721948934176622No Hit
ATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCC2600.20301397673147498No Hit
GACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT2490.1944249238697587No Hit
CCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATC2460.19208245490747247No Hit
AAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAG2350.1834934020457562No Hit
CGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA2280.178027641133755No Hit
ATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATA2170.16943858827203873No Hit
ACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGC1990.15538377449832122No Hit
GGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAA1860.14523307566174748No Hit
TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC1800.14054813773717498No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT1790.1397673147497462No Hit
TCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGACGAATTGCCAG1760.13742484578745998No Hit
GAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGAC1750.1366440228000312No Hit
TTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAA1750.1366440228000312No Hit
ACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCT1740.13586319981260248No Hit
TACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTA1730.13508237682517374No Hit
TTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTACG1710.13352073085031624No Hit
CCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGC1680.13117826188803No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG1560.12180838603888498No Hit
ACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC1540.1202467400640275No Hit
CGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGG1510.11790427110174125No Hit
AACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCC1470.11478097915202624No Hit
AGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGAT1430.11165768720231123No Hit
TTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTA1420.11087686421488248No Hit
AATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTT1410.11009604122745374No Hit
AGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGT1370.10697274927773874No Hit
ATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCT1360.10619192629030998No Hit
ATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTT1350.10541110330288123No Hit
TAGTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGG1340.10463028031545249No Hit
GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG1320.10306863434059498No Hit
ATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCT1290.10072616537830872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)