FastQCFastQC Report
Fri 15 Nov 2024
UHR_Rep1_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUHR_Rep1_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222009
Sequences flagged as poor quality0
Sequence length47-87
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG10590.4770076888774779No Hit
TGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTT6160.2774662288465783No Hit
TGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATA5880.2648541275353702No Hit
ATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGG5620.2531428906035341No Hit
GATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCA5540.24953943308604606No Hit
CTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAATCAT5350.24098122148201198No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG4550.20494664630713166No Hit
TATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAA4350.19593800251341162No Hit
CGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAATCATCT4340.19548757032372563No Hit
TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT3780.1702633677013094No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG3640.16395731704570535No Hit
CACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTTCAT3570.16080429171790334No Hit
TTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTG3550.1599034273385313No Hit
AAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCATGTGTAGTGCGCC3420.15404780887261327No Hit
GTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGC3380.15224608011386925No Hit
AGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGC3340.15044435135512524No Hit
ACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAAT3340.15044435135512524No Hit
GTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGA3330.14999391916543925No Hit
ATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATA3320.14954348697575323No Hit
GCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGAT3310.14909305478606724No Hit
TGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTG3190.1436878685098352No Hit
ACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAAC3180.14323743632014918No Hit
AGATTGCACCGTTGTTATAATCGACCCGGGCTGCGACAATCGGCCGTATG3150.14188613975109118No Hit
TGACTTGGCGACAGTTCATCATTAAAGATGAGGAGATCAGCTTCAAGCTC3090.13918354661297513No Hit
AACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCA2970.13377836033674312No Hit
TAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACC2950.13287749595737108No Hit
ACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGA2950.13287749595737108No Hit
CCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGA2820.12702187749145305No Hit
ATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGG2790.12567058092239503No Hit
CTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTT2790.12567058092239503No Hit
GCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTT2730.12296798778427902No Hit
AGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGG2710.12206712340490701No Hit
CACCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCGTCCCTATACGCGA2710.12206712340490701No Hit
TAAACGCGGTGACTAGGATGCTCATTTGAATGTCCCCAATTCGCGTCATC2710.12206712340490701No Hit
ATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCT2680.120715826835849No Hit
TTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAA2610.11756280150804699No Hit
GCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGT2600.11711236931836097No Hit
TCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGT2590.11666193712867497No Hit
TTCGTTTCCCCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAAAG2540.11440977618024495No Hit
GCGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGT2540.11440977618024495No Hit
ATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTC2510.11305847961118694No Hit
CGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGT2510.11305847961118694No Hit
CGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGT2470.11125675085244292No Hit
AAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAG2440.10990545428338491No Hit
CAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCA2430.10945502209369891No Hit
CTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTT2410.10855415771432689No Hit
TACTCACAATATCCTAAAAACGATCAGCCGGCAGGTTCATTTTAAACGCG2400.10810372552464088No Hit
GCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAATCATCTTCACT2380.10720286114526889No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA2340.10540113238652488No Hit
TAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAAA2330.10495070019683886No Hit
CTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGT2300.10359940362778086No Hit
CCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGACGCGGCAGTGCATA2300.10359940362778086No Hit
CAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTG2270.10224810705872285No Hit
ATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTA2260.10179767486903685No Hit
GGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTAT2240.10089681048966483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)