FastQCFastQC Report
Fri 15 Nov 2024
UHR_Rep1_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUHR_Rep1_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222009
Sequences flagged as poor quality0
Sequence length47-87
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTT8090.36439964145597703No Hit
ATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCC4550.20494664630713166No Hit
AAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATAT4230.19053281623717958No Hit
CATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATA4120.18557806215063352No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG3470.15629996982104327No Hit
ACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGC3470.15629996982104327No Hit
ACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATC3310.14909305478606724No Hit
GACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT3160.14233657194077717No Hit
CGACCGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTT3050.13738181785423112No Hit
GAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAG3040.13693138566454513No Hit
ATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATA3020.13603052128517312No Hit
TCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAA3010.1355800890954871No Hit
TTGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGG2870.12927403843988305No Hit
AAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAG2820.12702187749145305No Hit
TCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCA2820.12702187749145305No Hit
CGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGAAC2800.12612101311208104No Hit
GGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAG2780.12522014873270904No Hit
ATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCT2700.12161669121522099No Hit
AGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGT2630.11846366588741898No Hit
TAGTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGG2580.11621150493898895No Hit
TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC2560.11531064055961696No Hit
TTGTCGGCTATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACG2470.11125675085244292No Hit
AACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCC2450.1103558864730709No Hit
TGAGCTGGCTCAGCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGA2370.10675242895558287No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC2320.10450026800715286No Hit
CCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGG2300.10359940362778086No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA2270.10224810705872285No Hit
ACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATATATAGG2270.10224810705872285No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA2240.10089681048966483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)