FastQCFastQC Report
Fri 15 Nov 2024
UHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158392
Sequences flagged as poor quality0
Sequence length45-87
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG8260.5214909843931512No Hit
TGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATA5640.3560785898277691No Hit
TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT5560.3510278296883681No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG5300.3346128592353149No Hit
TGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTT4640.2929440880852568No Hit
TTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTG3710.23422900146472045No Hit
CGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAATCATCT3180.20076771554118894No Hit
GATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCA3130.19761099045406336No Hit
CTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAATCAT2990.18877216021011162No Hit
ATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGG2990.18877216021011162No Hit
GCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGT2760.1742512248093338No Hit
GCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTT2710.1710944997222082No Hit
CTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTT2610.16478104954795697No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG2560.16162432446083136No Hit
GTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGA2540.16036163442598111No Hit
ATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGG2430.15341683923430477No Hit
TATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAA2350.14836607909490376No Hit
GTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGC2350.14836607909490376No Hit
ATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCT2320.1464720440426284No Hit
GCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGAT2310.1458406990252033No Hit
CTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCG2280.14394666397292794No Hit
TACTCACAATATCCTAAAAACGATCAGCCGGCAGGTTCATTTTAAACGCG2260.14268397393807766No Hit
TTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAA2210.13952724885095208No Hit
AGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGC2170.13700186878125156No Hit
CGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGT2170.13700186878125156No Hit
TGACTTGGCGACAGTTCATCATTAAAGATGAGGAGATCAGCTTCAAGCTC2130.13447648871155107No Hit
GCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAATCATCTTCACT2110.13321379867670083No Hit
AAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCATGTGTAGTGCGCC2050.12942572857215012No Hit
TTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATC2040.12879438355472497No Hit
GCGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGT2030.12816303853729988No Hit
AAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAG2020.12753169351987476No Hit
CCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAATCATCTTCA2000.1262690034850245No Hit
CCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGA1910.1205868983281984No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC1890.11932420829334814No Hit
ACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGA1880.11869286327592303No Hit
CAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCT1870.11806151825849791No Hit
TGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTG1870.11806151825849791No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG1870.11806151825849791No Hit
ATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATA1830.1155361381887974No Hit
TTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTC1820.1149047931713723No Hit
CCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGACGCGGCAGTGCATA1790.11301075811909693No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTAT1760.11111672306682156No Hit
ACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAAC1750.11048537804939644No Hit
AGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGG1710.10795999797969594No Hit
AGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATAT1680.10606596292742057No Hit
GGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATA1680.10606596292742057No Hit
ATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTC1660.10480327289257034No Hit
GACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGC1630.10290923784029497No Hit
GTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAAACT1610.10164654780544471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)