FastQCFastQC Report
Fri 15 Nov 2024
UHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158392
Sequences flagged as poor quality0
Sequence length45-87
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTT6820.4305773018839335No Hit
ATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCC4030.25443204202232433No Hit
AAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATAT3600.22728420627304408No Hit
ACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGC3060.19319157533208747No Hit
GAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAG2890.1824587100358604No Hit
ACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATC2840.17930198494873478No Hit
CGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGAAC2570.16225566947825648No Hit
GGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAG2370.14962876912975404No Hit
GACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT2370.14962876912975404No Hit
AAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAG2280.14394666397292794No Hit
ATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATA2270.1433153189555028No Hit
ATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTG2270.1433153189555028No Hit
CGACCGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTT2260.14268397393807766No Hit
CATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATA2260.14268397393807766No Hit
CCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATC2150.13573917874640135No Hit
CGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA2010.1269003485024496No Hit
TCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCA1990.1256376584675994No Hit
TTGTCGGCTATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACG1930.12184958836304866No Hit
AGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGAT1860.11743017324107277No Hit
TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC1850.11679882822364766No Hit
TAGTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGG1810.11427344815394717No Hit
ACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATATATAGG1810.11427344815394717No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG1810.11427344815394717No Hit
TCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAA1780.11237941310167181No Hit
TTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTA1670.10543461790999545No Hit
AGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGT1650.1041719278751452No Hit
ATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCT1590.10038385777059447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)