FastQCFastQC Report
Fri 15 Nov 2024
UHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181227
Sequences flagged as poor quality0
Sequence length44-87
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG9790.5402064813741881No Hit
TGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATA5320.2935544924321431No Hit
TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT4830.2665165786554984No Hit
TGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTT4690.25879146043359985No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG4590.2532735188465295No Hit
GATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCA4130.22789098754600587No Hit
CTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAATCAT4030.22237304595893545No Hit
TTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTG4020.22182125180022846No Hit
ATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGG3380.18650642564297815No Hit
CGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAATCATCT3280.18098848405590778No Hit
TATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAA3210.17712592494495855No Hit
AAGGGGCAACTCGAGCTAATCTCCCCAGCGGCTAGCATGTGTAGTGCGCC2980.1644346592946967No Hit
GCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGT2900.16002030602504042No Hit
ATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGG2830.15615774691409118No Hit
GTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACTGA2820.15560595275538414No Hit
GCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTTCATCATTAAAGAT2770.15284698196184895No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG2720.15008801116831377No Hit
GCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTT2710.14953621700960673No Hit
GTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGC2670.1473290403747786No Hit
CTGACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTT2610.14401827542253637No Hit
ATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCT2550.14070751047029417No Hit
CCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGA2550.14070751047029417No Hit
TGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTG2540.14015571631158713No Hit
GCGCCAATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGT2510.138500333835466No Hit
TACTCACAATATCCTAAAAACGATCAGCCGGCAGGTTCATTTTAAACGCG2510.138500333835466No Hit
ACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAAT2390.1318788039309816No Hit
TTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAA2350.12967162729615345No Hit
AGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGC2340.1291198331374464No Hit
CGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGT2290.12636086234391122No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG2250.1241536857090831No Hit
GCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAATCATCTTCACT2210.12194650907425494No Hit
TGACTTGGCGACAGTTCATCATTAAAGATGAGGAGATCAGCTTCAAGCTC2190.12084292075684086No Hit
AACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCA2190.12084292075684086No Hit
CTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCG2190.12084292075684086No Hit
AGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATAT2120.11698036164589161No Hit
CACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGTTCAT2100.11587677332847754No Hit
TCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCGACAGT2090.11532497916977051No Hit
ACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGA2090.11532497916977051No Hit
AAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAG2080.11477318501106348No Hit
CCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGACGCGGCAGTGCATA2050.11311780253494236No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC2010.11091062590011423No Hit
GTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTAT2010.11091062590011423No Hit
TTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATC1990.10980703758270015No Hit
ATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATA1990.10980703758270015No Hit
ACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAAC1970.10870344926528608No Hit
ATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTC1950.107599860947872No Hit
CCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGGTCAATCATCTTCA1930.10649627263045794No Hit
CTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTT1930.10649627263045794No Hit
CGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGT1900.10484089015433681No Hit
TAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACC1880.10373730183692277No Hit
CAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATA1850.10208191936080165No Hit
TAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAAA1840.10153012520209462No Hit
CAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCA1840.10153012520209462No Hit
GACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGC1830.10097833104338756No Hit
GTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAAACT1830.10097833104338756No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)