FastQCFastQC Report
Fri 15 Nov 2024
UHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181227
Sequences flagged as poor quality0
Sequence length44-87
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTT6990.38570411693621814No Hit
ATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCC4060.2240284284350566No Hit
AAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATAT3740.20637101535643143No Hit
GACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT3260.17988489573849373No Hit
ACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGC3210.17712592494495855No Hit
ACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATC3040.16774542424693892No Hit
GAAGAGCTAGCATCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAG2950.1627792768185756No Hit
GGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAG2820.15560595275538414No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG2730.15063980532702081No Hit
TCCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCA2670.1473290403747786No Hit
CATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATA2620.1445700695812434No Hit
ATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATA2610.14401827542253637No Hit
CGACCGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTT2610.14401827542253637No Hit
CGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAAACGGAAC2560.1412593046290012No Hit
AAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAG2470.1362931572006379No Hit
TTGTCGGCTATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACG2140.11808394996330569No Hit
AGCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGT2120.11698036164589161No Hit
ACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCT2080.11477318501106348No Hit
TCTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGCGTCACACAAAA2070.11422139085235643No Hit
TAGTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGG2050.11311780253494236No Hit
TTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCCGATGCTGACGGCT2050.11311780253494236No Hit
ATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTG2010.11091062590011423No Hit
TGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC1940.10704806678916497No Hit
AACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCC1890.1042890959956298No Hit
CGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA1880.10373730183692277No Hit
ACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATATATAGG1840.10153012520209462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)