Sample sequence_duplication_levels per_base_n_content overrepresented_sequences total_deduplicated_percentage Total Sequences avg_sequence_length per_sequence_gc_content %GC per_sequence_quality_scores Sequences flagged as poor quality Sequence length sequence_length_distribution Filename adapter_content per_tile_sequence_quality median_sequence_length Encoding per_base_sequence_content basic_statistics File type per_base_sequence_quality HBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1 warn pass warn 54.54219612729243 117179.0 86.98092661654393 pass 49.0 pass 0.0 46-87 warn HBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz pass pass 87 Sanger / Illumina 1.9 pass pass Conventional base calls pass HBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2 warn pass warn 56.26264091688784 117179.0 86.9744066769643 warn 50.0 pass 0.0 46-87 warn HBR_Rep1_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2.fastq.gz pass pass 87 Sanger / Illumina 1.9 pass pass Conventional base calls pass HBR_Rep2_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1 warn pass warn 52.85238654803872 142968.0 86.97773627664932 pass 50.0 pass 0.0 44-87 warn HBR_Rep2_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz pass pass 87 Sanger / Illumina 1.9 pass pass Conventional base calls pass HBR_Rep2_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2 warn pass warn 54.63670191931062 142968.0 86.97075569358178 warn 50.0 pass 0.0 44-87 warn HBR_Rep2_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2.fastq.gz pass pass 87 Sanger / Illumina 1.9 pass pass Conventional base calls pass HBR_Rep3_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1 warn pass warn 54.02280003123292 128070.0 86.980869836808 pass 49.0 pass 0.0 48-87 warn HBR_Rep3_ERCC-Mix2_Build37-ErccTranscripts-chr22.read1.fastq.gz pass pass 87 Sanger / Illumina 1.9 pass pass Conventional base calls pass HBR_Rep3_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2 warn pass warn 55.74998047942531 128070.0 86.97656750214726 warn 50.0 pass 0.0 48-87 warn HBR_Rep3_ERCC-Mix2_Build37-ErccTranscripts-chr22.read2.fastq.gz pass pass 87 Sanger / Illumina 1.9 pass pass Conventional base calls pass UHR_Rep1_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1 fail pass warn 44.399551369539076 222009.0 86.98669873743857 pass 49.0 pass 0.0 47-87 warn UHR_Rep1_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz pass pass 87 Sanger / Illumina 1.9 pass pass Conventional base calls pass UHR_Rep1_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2 fail pass warn 48.02539065971978 222009.0 86.98127553387475 pass 49.0 pass 0.0 47-87 warn UHR_Rep1_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz pass pass 87 Sanger / Illumina 1.9 pass pass Conventional base calls pass UHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1 warn pass warn 50.75256326077074 158392.0 86.97963912318804 pass 48.0 pass 0.0 45-87 warn UHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz pass pass 87 Sanger / Illumina 1.9 pass pass Conventional base calls pass UHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2 warn pass warn 52.33281983938583 158392.0 86.97470831860196 pass 48.0 pass 0.0 45-87 warn UHR_Rep2_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz pass pass 87 Sanger / Illumina 1.9 pass pass Conventional base calls pass UHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1 fail pass warn 45.511430415997616 181227.0 86.98012989234496 pass 49.0 pass 0.0 44-87 warn UHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read1.fastq.gz pass pass 87 Sanger / Illumina 1.9 pass pass Conventional base calls pass UHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2 fail pass warn 47.007896174411094 181227.0 86.97404360277442 pass 49.0 pass 0.0 44-87 warn UHR_Rep3_ERCC-Mix1_Build37-ErccTranscripts-chr22.read2.fastq.gz pass pass 87 Sanger / Illumina 1.9 pass pass Conventional base calls pass