Sample adapter_cutting duplication insert_size summary read2_before_filtering read1_after_filtering read1_before_filtering read2_after_filtering filtering_result command SRR10045016_1 {'adapter_trimmed_reads': 41618, 'adapter_trimmed_bases': 256935, 'read1_adapter_sequence': 'unspecified', 'read2_adapter_sequence': 'unspecified', 'read1_adapter_counts': {'A': 628, 'C': 748, 'G': 767, 'T': 638, 'GG': 252, 'TG': 214, 'others': 17562}, 'read2_adapter_counts': {'A': 755, 'C': 711, 'G': 694, 'T': 621, 'AG': 215, 'CA': 220, 'others': 17593}} {'rate': 0.133445} {'peak': 85, 'unknown': 900468, 'histogram': [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 47, 46, 32, 34, 50, 42, 52, 62, 71, 65, 95, 87, 100, 144, 124, 153, 208, 238, 290, 354, 424, 442, 591, 673, 759, 875, 920, 1104, 1190, 1397, 1476, 1638, 1820, 2059, 2144, 2421, 2586, 2830, 2998, 3308, 3406, 3641, 4040, 4157, 4235, 4539, 4682, 5028, 5231, 5485, 5467, 5635, 5812, 6010, 6215, 0, 0, 0, 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0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0]} {'fastp_version': '0.23.4', 'sequencing': 'paired end (63 cycles + 63 cycles)', 'before_filtering': {'total_reads': 6046110, 'total_bases': 380849543, 'q20_bases': 360551638, 'q30_bases': 344195729, 'q20_rate': 0.946704, 'q30_rate': 0.903758, 'read1_mean_length': 62, 'read2_mean_length': 62, 'gc_content': 0.582952}, 'after_filtering': {'total_reads': 5921344, 'total_bases': 343088416, 'q20_bases': 330293646, 'q30_bases': 316830857, 'q20_rate': 0.962707, 'q30_rate': 0.923467, 'read1_mean_length': 57, 'read2_mean_length': 57, 'gc_content': 0.581702}} {'total_reads': 3023055, 'total_bases': 190420290, 'q20_bases': 176918135, 'q30_bases': 167404359, 'total_cycles': 63, 'quality_curves': {'A': [35.8732, 35.869, 35.8726, 35.9166, 35.9419, 35.9112, 35.9206, 35.9233, 35.8987, 35.8773, 35.847, 35.8497, 35.8158, 35.7842, 35.7857, 35.7576, 35.7484, 35.7318, 35.7055, 35.69, 35.6251, 35.622, 35.6222, 35.5882, 35.5522, 35.492, 35.4533, 35.4085, 35.3836, 35.2786, 35.2555, 35.2041, 35.1532, 35.1452, 35.1044, 35.0462, 35.011, 34.9733, 34.9233, 34.9211, 34.9284, 34.9178, 34.8222, 34.722, 34.6627, 34.5904, 34.4902, 34.4389, 34.3749, 34.2013, 34.025, 33.8114, 33.6763, 33.5463, 33.5085, 33.3333, 33.1316, 32.9742, 32.5891, 32.2239, 31.8129, 31.0187, 20.4591], 'T': [36.425, 36.4815, 36.4284, 36.4415, 36.4226, 36.4212, 36.3736, 36.3745, 36.3374, 36.2943, 36.2769, 36.2399, 36.2226, 36.1946, 36.1618, 36.1427, 36.1077, 36.0861, 36.043, 36.0031, 35.9576, 35.9572, 35.9283, 35.8649, 35.8314, 35.7504, 35.7445, 35.6691, 35.6138, 35.5845, 35.5223, 35.5099, 35.4285, 35.4041, 35.3288, 35.3149, 35.239, 35.19, 35.1318, 35.0774, 35.0847, 35.0884, 34.9986, 34.9277, 34.8193, 34.7225, 34.6524, 34.5909, 34.481, 34.3509, 34.203, 33.9938, 33.8394, 33.775, 33.6845, 33.5635, 33.3291, 33.1861, 32.8309, 32.4476, 32.0755, 31.3923, 21.4657], 'C': [36.3194, 36.3385, 36.3417, 36.3321, 36.3231, 36.3258, 36.3318, 36.3245, 36.3154, 36.2616, 36.2509, 36.2439, 36.2341, 36.2204, 36.2108, 36.1873, 36.1807, 36.1518, 36.137, 36.0935, 36.0764, 36.0692, 36.0322, 36.04, 35.9906, 35.9474, 35.9257, 35.894, 35.8237, 35.8031, 35.7526, 35.7027, 35.6995, 35.65, 35.6167, 35.6038, 35.5386, 35.4816, 35.4285, 35.4211, 35.3847, 35.3794, 35.3108, 35.2385, 35.1685, 35.0863, 34.9739, 34.9356, 34.7956, 34.703, 34.6103, 34.4305, 34.2216, 34.1537, 34.036, 33.9075, 33.6933, 33.5034, 33.2038, 32.8589, 32.4243, 31.7661, 26.356], 'G': [35.7409, 35.7703, 35.7757, 35.7891, 35.7883, 35.8102, 35.811, 35.7893, 35.746, 35.7392, 35.7139, 35.6922, 35.6832, 35.6796, 35.6531, 35.6299, 35.6122, 35.5935, 35.5677, 35.5364, 35.5066, 35.4994, 35.4646, 35.4512, 35.4122, 35.3539, 35.336, 35.2756, 35.2337, 35.1656, 35.1389, 35.1233, 35.0837, 35.0631, 34.9968, 34.956, 34.9055, 34.8611, 34.8119, 34.7997, 34.751, 34.7785, 34.6999, 34.6207, 34.5399, 34.4627, 34.3574, 34.3219, 34.2116, 34.1065, 33.9218, 33.7424, 33.5528, 33.4522, 33.421, 33.1896, 32.9621, 32.8798, 32.4608, 32.0183, 31.5938, 30.8835, 25.1657], 'mean': [36.0803, 36.0939, 36.0933, 36.0991, 36.1019, 36.1022, 36.0844, 36.091, 36.0604, 36.028, 36.0048, 35.986, 35.9733, 35.9544, 35.943, 35.9055, 35.9072, 35.8784, 35.8515, 35.8118, 35.7812, 35.7702, 35.7552, 35.7346, 35.6928, 35.6375, 35.612, 35.5503, 35.5133, 35.4602, 35.4058, 35.3851, 35.3358, 35.302, 35.2643, 35.2333, 35.1785, 35.1239, 35.0765, 35.0635, 35.043, 35.0377, 34.9555, 34.8684, 34.7868, 34.7084, 34.6276, 34.5798, 34.4726, 34.351, 34.2004, 33.9863, 33.8293, 33.7408, 33.6647, 33.4983, 33.2923, 33.1452, 32.7789, 32.3992, 31.985, 31.2742, 23.8901]}, 'content_curves': {'A': [0.211953, 0.216822, 0.214441, 0.21809, 0.225522, 0.214866, 0.217646, 0.219925, 0.217663, 0.220055, 0.224055, 0.219559, 0.218088, 0.22188, 0.214374, 0.21678, 0.220893, 0.216761, 0.218585, 0.220504, 0.217644, 0.217593, 0.221021, 0.214979, 0.219776, 0.220313, 0.214535, 0.218636, 0.221461, 0.217147, 0.220605, 0.222045, 0.216908, 0.21849, 0.221899, 0.214952, 0.215374, 0.222661, 0.218826, 0.220141, 0.224868, 0.21596, 0.219068, 0.220262, 0.218723, 0.217695, 0.223764, 0.217835, 0.219798, 0.220816, 0.214626, 0.215844, 0.220057, 0.215064, 0.218364, 0.224123, 0.215107, 0.219494, 0.222011, 0.215952, 0.219102, 0.220567, 0.208009], 'T': [0.205161, 0.1972, 0.196833, 0.189947, 0.192402, 0.191134, 0.188882, 0.197437, 0.194855, 0.19164, 0.198808, 0.194306, 0.191826, 0.196586, 0.195011, 0.193322, 0.19451, 0.198367, 0.19284, 0.195932, 0.191765, 0.188745, 0.195065, 0.189664, 0.192337, 0.195721, 0.193413, 0.192319, 0.196092, 0.191781, 0.190211, 0.19571, 0.193353, 0.192244, 0.194741, 0.194748, 0.190275, 0.193515, 0.192034, 0.189074, 0.191213, 0.192317, 0.191461, 0.197159, 0.190783, 0.190998, 0.194127, 0.191991, 0.190157, 0.196089, 0.193537, 0.189692, 0.19499, 0.194513, 0.190503, 0.194109, 0.194462, 0.190619, 0.195324, 0.192609, 0.191747, 0.19449, 0.182771], 'C': [0.298821, 0.298736, 0.297636, 0.29397, 0.29497, 0.297642, 0.295999, 0.296675, 0.298366, 0.296223, 0.293201, 0.29611, 0.297244, 0.295731, 0.298953, 0.298555, 0.297443, 0.299843, 0.298312, 0.298619, 0.297381, 0.297834, 0.297784, 0.304488, 0.299303, 0.299026, 0.301991, 0.299405, 0.296839, 0.305235, 0.299663, 0.295531, 0.301138, 0.29825, 0.2974, 0.303225, 0.303243, 0.299164, 0.303151, 0.301199, 0.299607, 0.303444, 0.299859, 0.299703, 0.301688, 0.30172, 0.299484, 0.302221, 0.301233, 0.297845, 0.304321, 0.301284, 0.300049, 0.30579, 0.300919, 0.299605, 0.305119, 0.302251, 0.297941, 0.305273, 0.30194, 0.300167, 0.320594], 'G': [0.284011, 0.287012, 0.291086, 0.297951, 0.287081, 0.296357, 0.297149, 0.285901, 0.288998, 0.292002, 0.283691, 0.289712, 0.292662, 0.285517, 0.291529, 0.29082, 0.287137, 0.284725, 0.290032, 0.284461, 0.292996, 0.295469, 0.28602, 0.290757, 0.288468, 0.284881, 0.289873, 0.289183, 0.285511, 0.28569, 0.289049, 0.286623, 0.288146, 0.290423, 0.285797, 0.286819, 0.290951, 0.284312, 0.28574, 0.289507, 0.284264, 0.28789, 0.289261, 0.282253, 0.288104, 0.288974, 0.282581, 0.287809, 0.288748, 0.285186, 0.287282, 0.292361, 0.284674, 0.28435, 0.289875, 0.281804, 0.28529, 0.287565, 0.284591, 0.286071, 0.28721, 0.284675, 0.288545], 'N': [5.45805e-05, 0.0002299, 3.96949e-06, 4.20105e-05, 2.58017e-05, 3.30791e-07, 0.000323183, 6.21887e-05, 0.000118092, 8.07131e-05, 0.000244785, 0.000312928, 0.00017995, 0.000285473, 0.000132647, 0.000522319, 1.65396e-05, 0.000304659, 0.000231223, 0.000483948, 0.000213691, 0.000358247, 0.000111146, 0.000112138, 0.000116769, 5.78891e-05, 0.000187564, 0.000457503, 9.75886e-05, 0.00014655, 0.000472734, 8.99822e-05, 0.000454876, 0.000593162, 0.000162434, 0.000256057, 0.000157473, 0.0003477, 0.000248453, 7.84071e-05, 4.83017e-05, 0.000389395, 0.000352344, 0.000622976, 0.00070371, 0.000614057, 4.36726e-05, 0.000145247, 6.3526e-05, 6.41893e-05, 0.000233273, 0.000818973, 0.00023098, 0.000283285, 0.000339567, 0.000357799, 2.28414e-05, 7.21657e-05, 0.000133076, 9.43451e-05, 0, 0.000101959, 8.11037e-05], 'GC': [0.582832, 0.585748, 0.588722, 0.591921, 0.582051, 0.593999, 0.593148, 0.582576, 0.587364, 0.588224, 0.576892, 0.585822, 0.589906, 0.581249, 0.590482, 0.589375, 0.58458, 0.584567, 0.588344, 0.58308, 0.590377, 0.593303, 0.583803, 0.595244, 0.58777, 0.583908, 0.591864, 0.588588, 0.58235, 0.590925, 0.588711, 0.582154, 0.589284, 0.588673, 0.583197, 0.590044, 0.594194, 0.583476, 0.588891, 0.590706, 0.583871, 0.591334, 0.589119, 0.581957, 0.589791, 0.590694, 0.582065, 0.59003, 0.589981, 0.583031, 0.591603, 0.593645, 0.584722, 0.59014, 0.590794, 0.581409, 0.590408, 0.589815, 0.582532, 0.591344, 0.589151, 0.584842, 0.609139]}, 'kmer_count': {'AAAAA': 354394, 'AAAAT': 129484, 'AAAAC': 156433, 'AAAAG': 180033, 'AAATA': 97191, 'AAATT': 99829, 'AAATC': 88264, 'AAATG': 117064, 'AAACA': 169678, 'AAACT': 119546, 'AAACC': 142698, 'AAACG': 68752, 'AAAGA': 242442, 'AAAGT': 130961, 'AAAGC': 164225, 'AAAGG': 158759, 'AATAA': 64632, 'AATAT': 59674, 'AATAC': 63988, 'AATAG': 42203, 'AATTA': 55807, 'AATTT': 101173, 'AATTC': 77530, 'AATTG': 62364, 'AATCA': 64435, 'AATCT': 77784, 'AATCC': 98797, 'AATCG': 26287, 'AATGA': 105032, 'AATGT': 109562, 'AATGC': 90035, 'AATGG': 128438, 'AACAA': 203931, 'AACAT': 166802, 'AACAC': 120434, 'AACAG': 174617, 'AACTA': 60291, 'AACTT': 127451, 'AACTC': 111113, 'AACTG': 151520, 'AACCA': 174829, 'AACCT': 154328, 'AACCC': 186334, 'AACCG': 78221, 'AACGA': 72892, 'AACGT': 77208, 'AACGC': 82623, 'AACGG': 94728, 'AAGAA': 450036, 'AAGAT': 254075, 'AAGAC': 229630, 'AAGAG': 290094, 'AAGTA': 106358, 'AAGTT': 151896, 'AAGTC': 149612, 'AAGTG': 153027, 'AAGCA': 209186, 'AAGCT': 241185, 'AAGCC': 268683, 'AAGCG': 137128, 'AAGGA': 335262, 'AAGGT': 183623, 'AAGGC': 253038, 'AAGGG': 209402, 'ATAAA': 81490, 'ATAAT': 36677, 'ATAAC': 42180, 'ATAAG': 41941, 'ATATA': 40045, 'ATATT': 58250, 'ATATC': 50544, 'ATATG': 48896, 'ATACA': 66038, 'ATACT': 49372, 'ATACC': 55100, 'ATACG': 24431, 'ATAGA': 44075, 'ATAGT': 33792, 'ATAGC': 33569, 'ATAGG': 38619, 'ATTAA': 63289, 'ATTAT': 47725, 'ATTAC': 64963, 'ATTAG': 26225, 'ATTTA': 66203, 'ATTTT': 146519, 'ATTTC': 112599, 'ATTTG': 93330, 'ATTCA': 93914, 'ATTCT': 95126, 'ATTCC': 101639, 'ATTCG': 46854, 'ATTGA': 119665, 'ATTGT': 85558, 'ATTGC': 80272, 'ATTGG': 68628, 'ATCAA': 181192, 'ATCAT': 154451, 'ATCAC': 120671, 'ATCAG': 106619, 'ATCTA': 70018, 'ATCTT': 141192, 'ATCTC': 140471, 'ATCTG': 157921, 'ATCCA': 171990, 'ATCCT': 191250, 'ATCCC': 170659, 'ATCCG': 129498, 'ATCGA': 102606, 'ATCGT': 65286, 'ATCGC': 73429, 'ATCGG': 66578, 'ATGAA': 193074, 'ATGAT': 157032, 'ATGAC': 143326, 'ATGAG': 218763, 'ATGTA': 66082, 'ATGTT': 95933, 'ATGTC': 130268, 'ATGTG': 176891, 'ATGCA': 131627, 'ATGCT': 133781, 'ATGCC': 195259, 'ATGCG': 65983, 'ATGGA': 207007, 'ATGGT': 145080, 'ATGGC': 229067, 'ATGGG': 186604, 'ACAAA': 180462, 'ACAAT': 69123, 'ACAAC': 174512, 'ACAAG': 306255, 'ACATA': 41214, 'ACATT': 98723, 'ACATC': 228648, 'ACATG': 178220, 'ACACA': 159849, 'ACACT': 98965, 'ACACC': 186394, 'ACACG': 96282, 'ACAGA': 204569, 'ACAGT': 127010, 'ACAGC': 283725, 'ACAGG': 197047, 'ACTAA': 46265, 'ACTAT': 58524, 'ACTAC': 127781, 'ACTAG': 23328, 'ACTTA': 44112, 'ACTTT': 126897, 'ACTTC': 206378, 'ACTTG': 95889, 'ACTCA': 113985, 'ACTCT': 112694, 'ACTCC': 194496, 'ACTCG': 65743, 'ACTGA': 143781, 'ACTGT': 129261, 'ACTGC': 192299, 'ACTGG': 215017, 'ACCAA': 224776, 'ACCAT': 206370, 'ACCAC': 207792, 'ACCAG': 337849, 'ACCTA': 90219, 'ACCTT': 142623, 'ACCTC': 238837, 'ACCTG': 374661, 'ACCCA': 256372, 'ACCCT': 229029, 'ACCCC': 368138, 'ACCCG': 136262, 'ACCGA': 87494, 'ACCGT': 83199, 'ACCGC': 195294, 'ACCGG': 146927, 'ACGAA': 43594, 'ACGAT': 53052, 'ACGAC': 115000, 'ACGAG': 200375, 'ACGTA': 24435, 'ACGTT': 31665, 'ACGTC': 86854, 'ACGTG': 155728, 'ACGCA': 98163, 'ACGCT': 140283, 'ACGCC': 192653, 'ACGCG': 71085, 'ACGGA': 138480, 'ACGGT': 61555, 'ACGGC': 187547, 'ACGGG': 137529, 'AGAAA': 278876, 'AGAAT': 119336, 'AGAAC': 232070, 'AGAAG': 539201, 'AGATA': 60607, 'AGATT': 114407, 'AGATC': 242471, 'AGATG': 279249, 'AGACA': 201537, 'AGACT': 139782, 'AGACC': 245590, 'AGACG': 158694, 'AGAGA': 265168, 'AGAGT': 139670, 'AGAGC': 269684, 'AGAGG': 316238, 'AGTAA': 42531, 'AGTAT': 79022, 'AGTAC': 140632, 'AGTAG': 53845, 'AGTTA': 54722, 'AGTTT': 128005, 'AGTTC': 199644, 'AGTTG': 88668, 'AGTCA': 117175, 'AGTCT': 101336, 'AGTCC': 189374, 'AGTCG': 70384, 'AGTGA': 174989, 'AGTGT': 111122, 'AGTGC': 177140, 'AGTGG': 235095, 'AGCAA': 176225, 'AGCAT': 118260, 'AGCAC': 229316, 'AGCAG': 445791, 'AGCTA': 82517, 'AGCTT': 136053, 'AGCTC': 268796, 'AGCTG': 503804, 'AGCCA': 337105, 'AGCCT': 316458, 'AGCCC': 428816, 'AGCCG': 232374, 'AGCGA': 115526, 'AGCGT': 92393, 'AGCGC': 202127, 'AGCGG': 267467, 'AGGAA': 259299, 'AGGAT': 172309, 'AGGAC': 325944, 'AGGAG': 596668, 'AGGTA': 65495, 'AGGTT': 104060, 'AGGTC': 178855, 'AGGTG': 321508, 'AGGCA': 221740, 'AGGCT': 234041, 'AGGCC': 443997, 'AGGCG': 194499, 'AGGGA': 186562, 'AGGGT': 152922, 'AGGGC': 314460, 'AGGGG': 231342, 'TAAAA': 99144, 'TAAAT': 52201, 'TAAAC': 50688, 'TAAAG': 98230, 'TAATA': 23582, 'TAATT': 52962, 'TAATC': 33862, 'TAATG': 58299, 'TAACA': 59711, 'TAACT': 52831, 'TAACC': 46419, 'TAACG': 16832, 'TAAGA': 71989, 'TAAGT': 35365, 'TAAGC': 47499, 'TAAGG': 50061, 'TATAA': 38365, 'TATAT': 48134, 'TATAC': 31867, 'TATAG': 25225, 'TATTA': 40412, 'TATTT': 119594, 'TATTC': 52607, 'TATTG': 56097, 'TATCA': 56965, 'TATCT': 76233, 'TATCC': 60303, 'TATCG': 19646, 'TATGA': 94025, 'TATGT': 75863, 'TATGC': 73604, 'TATGG': 89299, 'TACAA': 149830, 'TACAT': 86923, 'TACAC': 83020, 'TACAG': 112067, 'TACTA': 50407, 'TACTT': 77368, 'TACTC': 58180, 'TACTG': 101015, 'TACCA': 119380, 'TACCT': 129267, 'TACCC': 108157, 'TACCG': 87450, 'TACGA': 90943, 'TACGT': 34527, 'TACGC': 59365, 'TACGG': 70789, 'TAGAA': 54679, 'TAGAT': 32705, 'TAGAC': 42449, 'TAGAG': 74198, 'TAGTA': 23730, 'TAGTT': 31383, 'TAGTC': 34711, 'TAGTG': 51955, 'TAGCA': 47076, 'TAGCT': 55403, 'TAGCC': 66745, 'TAGCG': 22292, 'TAGGA': 58747, 'TAGGT': 28585, 'TAGGC': 41384, 'TAGGG': 45190, 'TTAAA': 93156, 'TTAAT': 54235, 'TTAAC': 51720, 'TTAAG': 61642, 'TTATA': 41335, 'TTATT': 89453, 'TTATC': 47536, 'TTATG': 66536, 'TTACA': 71038, 'TTACT': 64062, 'TTACC': 57016, 'TTACG': 18693, 'TTAGA': 40269, 'TTAGT': 34283, 'TTAGC': 50802, 'TTAGG': 40251, 'TTTAA': 96763, 'TTTAT': 106151, 'TTTAC': 53070, 'TTTAG': 48531, 'TTTTA': 128693, 'TTTTT': 288019, 'TTTTC': 134552, 'TTTTG': 160317, 'TTTCA': 122988, 'TTTCT': 173623, 'TTTCC': 167492, 'TTTCG': 50040, 'TTTGA': 156207, 'TTTGT': 153153, 'TTTGC': 136437, 'TTTGG': 179570, 'TTCAA': 176546, 'TTCAT': 132968, 'TTCAC': 153029, 'TTCAG': 174058, 'TTCTA': 92863, 'TTCTT': 164411, 'TTCTC': 214969, 'TTCTG': 211314, 'TTCCA': 205008, 'TTCCT': 279288, 'TTCCC': 224702, 'TTCCG': 109118, 'TTCGA': 101588, 'TTCGT': 67924, 'TTCGC': 64149, 'TTCGG': 92821, 'TTGAA': 123345, 'TTGAT': 80999, 'TTGAC': 114861, 'TTGAG': 138445, 'TTGTA': 66332, 'TTGTT': 98915, 'TTGTC': 102373, 'TTGTG': 160460, 'TTGCA': 106311, 'TTGCT': 119714, 'TTGCC': 159342, 'TTGCG': 47576, 'TTGGA': 139414, 'TTGGT': 87825, 'TTGGC': 146322, 'TTGGG': 160868, 'TCAAA': 137322, 'TCAAT': 67043, 'TCAAC': 190135, 'TCAAG': 296412, 'TCATA': 43119, 'TCATT': 93791, 'TCATC': 241538, 'TCATG': 196561, 'TCACA': 128000, 'TCACT': 139967, 'TCACC': 274928, 'TCACG': 90420, 'TCAGA': 176915, 'TCAGT': 120608, 'TCAGC': 231158, 'TCAGG': 187433, 'TCTAA': 37658, 'TCTAT': 63497, 'TCTAC': 157203, 'TCTAG': 60656, 'TCTTA': 55341, 'TCTTT': 163287, 'TCTTC': 266198, 'TCTTG': 118545, 'TCTCA': 164556, 'TCTCT': 194237, 'TCTCC': 297796, 'TCTCG': 91457, 'TCTGA': 153107, 'TCTGT': 181157, 'TCTGC': 251441, 'TCTGG': 292383, 'TCCAA': 193601, 'TCCAT': 134993, 'TCCAC': 252783, 'TCCAG': 355857, 'TCCTA': 97228, 'TCCTT': 169733, 'TCCTC': 322820, 'TCCTG': 456092, 'TCCCA': 272874, 'TCCCT': 249842, 'TCCCC': 378501, 'TCCCG': 185635, 'TCCGA': 105130, 'TCCGT': 83374, 'TCCGC': 191709, 'TCCGG': 186501, 'TCGAA': 49166, 'TCGAT': 37564, 'TCGAC': 97899, 'TCGAG': 144194, 'TCGTA': 25537, 'TCGTT': 26204, 'TCGTC': 84698, 'TCGTG': 135665, 'TCGCA': 55483, 'TCGCT': 83752, 'TCGCC': 140120, 'TCGCG': 65349, 'TCGGA': 101839, 'TCGGT': 64048, 'TCGGC': 156586, 'TCGGG': 145227, 'TGAAA': 152396, 'TGAAT': 92472, 'TGAAC': 167708, 'TGAAG': 381259, 'TGATA': 51568, 'TGATT': 81181, 'TGATC': 141530, 'TGATG': 228228, 'TGACA': 169444, 'TGACT': 120766, 'TGACC': 268325, 'TGACG': 104176, 'TGAGA': 174712, 'TGAGT': 123983, 'TGAGC': 257937, 'TGAGG': 305277, 'TGTAA': 75122, 'TGTAT': 71815, 'TGTAC': 101816, 'TGTAG': 57960, 'TGTTA': 59105, 'TGTTT': 149894, 'TGTTC': 145543, 'TGTTG': 119723, 'TGTCA': 140445, 'TGTCT': 154514, 'TGTCC': 235095, 'TGTCG': 72418, 'TGTGA': 196212, 'TGTGT': 182110, 'TGTGC': 229165, 'TGTGG': 310487, 'TGCAA': 134860, 'TGCAT': 97895, 'TGCAC': 171749, 'TGCAG': 399972, 'TGCTA': 69870, 'TGCTT': 136022, 'TGCTC': 205055, 'TGCTG': 504351, 'TGCCA': 281720, 'TGCCT': 299754, 'TGCCC': 410532, 'TGCCG': 189865, 'TGCGA': 78160, 'TGCGT': 80696, 'TGCGC': 177817, 'TGCGG': 186422, 'TGGAA': 205657, 'TGGAT': 167458, 'TGGAC': 334021, 'TGGAG': 542757, 'TGGTA': 72333, 'TGGTT': 105397, 'TGGTC': 200393, 'TGGTG': 381032, 'TGGCA': 239991, 'TGGCT': 285779, 'TGGCC': 489789, 'TGGCG': 207039, 'TGGGA': 269110, 'TGGGT': 179472, 'TGGGC': 396894, 'TGGGG': 355924, 'CAAAA': 174910, 'CAAAT': 108647, 'CAAAC': 134287, 'CAAAG': 225044, 'CAATA': 50027, 'CAATT': 57532, 'CAATC': 65525, 'CAATG': 124802, 'CAACA': 255106, 'CAACT': 135127, 'CAACC': 222050, 'CAACG': 131170, 'CAAGA': 425870, 'CAAGT': 206832, 'CAAGC': 289839, 'CAAGG': 408514, 'CATAA': 44489, 'CATAT': 39906, 'CATAC': 51207, 'CATAG': 43141, 'CATTA': 56853, 'CATTT': 103834, 'CATTC': 103723, 'CATTG': 146674, 'CATCA': 288478, 'CATCT': 235970, 'CATCC': 303135, 'CATCG': 179320, 'CATGA': 206446, 'CATGT': 150949, 'CATGC': 187142, 'CATGG': 304587, 'CACAA': 153944, 'CACAT': 114401, 'CACAC': 176264, 'CACAG': 282575, 'CACTA': 75210, 'CACTT': 131694, 'CACTC': 139714, 'CACTG': 246093, 'CACCA': 365941, 'CACCT': 309197, 'CACCC': 382392, 'CACCG': 198306, 'CACGA': 98243, 'CACGT': 88187, 'CACGC': 191891, 'CACGG': 178744, 'CAGAA': 283574, 'CAGAT': 201877, 'CAGAC': 237028, 'CAGAG': 352814, 'CAGTA': 92054, 'CAGTT': 148970, 'CAGTC': 139101, 'CAGTG': 295745, 'CAGCA': 429065, 'CAGCT': 378825, 'CAGCC': 590965, 'CAGCG': 274550, 'CAGGA': 355246, 'CAGGT': 230070, 'CAGGC': 380568, 'CAGGG': 312855, 'CTAAA': 66766, 'CTAAT': 35731, 'CTAAC': 35342, 'CTAAG': 64638, 'CTATA': 37380, 'CTATT': 62981, 'CTATC': 68144, 'CTATG': 128527, 'CTACA': 186919, 'CTACT': 99961, 'CTACC': 210153, 'CTACG': 155476, 'CTAGA': 57570, 'CTAGT': 37301, 'CTAGC': 54145, 'CTAGG': 53222, 'CTTAA': 56502, 'CTTAT': 53895, 'CTTAC': 47611, 'CTTAG': 54195, 'CTTTA': 59316, 'CTTTT': 163809, 'CTTTC': 163276, 'CTTTG': 213252, 'CTTCA': 278259, 'CTTCT': 266759, 'CTTCC': 392634, 'CTTCG': 150402, 'CTTGA': 111701, 'CTTGT': 104860, 'CTTGC': 128138, 'CTTGG': 165584, 'CTCAA': 203693, 'CTCAT': 165467, 'CTCAC': 213020, 'CTCAG': 272388, 'CTCTA': 88169, 'CTCTT': 174877, 'CTCTC': 229744, 'CTCTG': 314571, 'CTCCA': 360501, 'CTCCT': 355478, 'CTCCC': 428988, 'CTCCG': 214865, 'CTCGA': 70489, 'CTCGT': 65842, 'CTCGC': 122898, 'CTCGG': 200859, 'CTGAA': 262168, 'CTGAT': 155415, 'CTGAC': 212540, 'CTGAG': 318959, 'CTGTA': 107980, 'CTGTT': 151458, 'CTGTC': 223201, 'CTGTG': 343751, 'CTGCA': 366949, 'CTGCT': 438776, 'CTGCC': 564149, 'CTGCG': 263842, 'CTGGA': 541296, 'CTGGT': 290522, 'CTGGC': 487664, 'CTGGG': 540348, 'CCAAA': 184741, 'CCAAT': 89562, 'CCAAC': 242355, 'CCAAG': 434127, 'CCATA': 63555, 'CCATT': 125563, 'CCATC': 335305, 'CCATG': 308345, 'CCACA': 270717, 'CCACT': 209679, 'CCACC': 507229, 'CCACG': 226430, 'CCAGA': 387930, 'CCAGT': 232660, 'CCAGC': 609789, 'CCAGG': 515869, 'CCTAA': 63846, 'CCTAT': 94567, 'CCTAC': 203190, 'CCTAG': 69896, 'CCTTA': 68482, 'CCTTT': 167996, 'CCTTC': 359934, 'CCTTG': 177619, 'CCTCA': 335170, 'CCTCT': 289965, 'CCTCC': 532778, 'CCTCG': 193828, 'CCTGA': 323005, 'CCTGT': 248283, 'CCTGC': 607437, 'CCTGG': 749775, 'CCCAA': 257258, 'CCCAT': 196427, 'CCCAC': 389369, 'CCCAG': 618430, 'CCCTA': 125105, 'CCCTT': 225173, 'CCCTC': 383934, 'CCCTG': 582631, 'CCCCA': 521744, 'CCCCT': 418565, 'CCCCC': 833457, 'CCCCG': 380474, 'CCCGA': 204059, 'CCCGT': 131062, 'CCCGC': 340385, 'CCCGG': 382566, 'CCGAA': 82472, 'CCGAT': 57105, 'CCGAC': 174569, 'CCGAG': 291951, 'CCGTA': 37741, 'CCGTT': 52329, 'CCGTC': 147833, 'CCGTG': 203727, 'CCGCA': 226424, 'CCGCT': 218250, 'CCGCC': 486389, 'CCGCG': 230133, 'CCGGA': 222475, 'CCGGT': 84325, 'CCGGC': 345659, 'CCGGG': 381489, 'CGAAA': 42230, 'CGAAT': 42126, 'CGAAC': 52683, 'CGAAG': 91586, 'CGATA': 23629, 'CGATT': 39639, 'CGATC': 41663, 'CGATG': 99261, 'CGACA': 128174, 'CGACT': 91705, 'CGACC': 158409, 'CGACG': 122684, 'CGAGA': 198027, 'CGAGT': 120234, 'CGAGC': 208852, 'CGAGG': 313978, 'CGTAA': 24634, 'CGTAT': 18085, 'CGTAC': 40744, 'CGTAG': 26097, 'CGTTA': 12248, 'CGTTT': 42418, 'CGTTC': 50649, 'CGTTG': 51489, 'CGTCA': 123425, 'CGTCT': 118816, 'CGTCC': 148130, 'CGTCG': 59804, 'CGTGA': 107049, 'CGTGT': 100902, 'CGTGC': 186554, 'CGTGG': 281771, 'CGCAA': 136602, 'CGCAT': 108296, 'CGCAC': 123258, 'CGCAG': 182612, 'CGCTA': 75333, 'CGCTT': 103539, 'CGCTC': 152688, 'CGCTG': 299873, 'CGCCA': 268627, 'CGCCT': 255862, 'CGCCC': 331556, 'CGCCG': 269796, 'CGCGA': 91403, 'CGCGT': 71042, 'CGCGC': 176837, 'CGCGG': 202217, 'CGGAA': 157612, 'CGGAT': 76798, 'CGGAC': 152597, 'CGGAG': 296152, 'CGGTA': 31598, 'CGGTT': 48676, 'CGGTC': 83739, 'CGGTG': 139298, 'CGGCA': 226729, 'CGGCT': 215470, 'CGGCC': 420552, 'CGGCG': 292980, 'CGGGA': 238215, 'CGGGT': 108851, 'CGGGC': 300701, 'CGGGG': 302341, 'GAAAA': 193081, 'GAAAT': 114479, 'GAAAC': 158835, 'GAAAG': 193416, 'GAATA': 59865, 'GAATT': 87332, 'GAATC': 79840, 'GAATG': 132987, 'GAACA': 181207, 'GAACT': 142916, 'GAACC': 182030, 'GAACG': 110492, 'GAAGA': 484571, 'GAAGT': 185694, 'GAAGC': 354458, 'GAAGG': 362572, 'GATAA': 55068, 'GATAT': 50999, 'GATAC': 47672, 'GATAG': 38876, 'GATTA': 49449, 'GATTT': 95475, 'GATTC': 103871, 'GATTG': 88842, 'GATCA': 152605, 'GATCT': 118126, 'GATCC': 202154, 'GATCG': 82416, 'GATGA': 305310, 'GATGT': 133369, 'GATGC': 177178, 'GATGG': 244483, 'GACAA': 224215, 'GACAT': 177851, 'GACAC': 162484, 'GACAG': 245221, 'GACTA': 70107, 'GACTT': 137039, 'GACTC': 177772, 'GACTG': 180499, 'GACCA': 315673, 'GACCT': 251645, 'GACCC': 318492, 'GACCG': 150276, 'GACGA': 150472, 'GACGT': 98898, 'GACGC': 168627, 'GACGG': 180977, 'GAGAA': 383118, 'GAGAT': 209010, 'GAGAC': 237402, 'GAGAG': 271163, 'GAGTA': 94019, 'GAGTT': 139155, 'GAGTC': 154702, 'GAGTG': 197224, 'GAGCA': 283396, 'GAGCT': 313380, 'GAGCC': 391321, 'GAGCG': 243845, 'GAGGA': 603898, 'GAGGT': 227597, 'GAGGC': 419217, 'GAGGG': 320055, 'GTAAA': 58585, 'GTAAT': 42010, 'GTAAC': 46891, 'GTAAG': 37185, 'GTATA': 24822, 'GTATT': 58429, 'GTATC': 46891, 'GTATG': 87790, 'GTACA': 107324, 'GTACT': 72832, 'GTACC': 122063, 'GTACG': 56185, 'GTAGA': 61952, 'GTAGT': 35783, 'GTAGC': 53750, 'GTAGG': 41489, 'GTTAA': 44203, 'GTTAT': 37030, 'GTTAC': 45562, 'GTTAG': 36012, 'GTTTA': 48753, 'GTTTT': 113461, 'GTTTC': 103939, 'GTTTG': 156817, 'GTTCA': 138888, 'GTTCT': 146370, 'GTTCC': 157973, 'GTTCG': 78739, 'GTTGA': 69550, 'GTTGT': 83360, 'GTTGC': 87087, 'GTTGG': 119489, 'GTCAA': 128723, 'GTCAT': 119994, 'GTCAC': 145883, 'GTCAG': 161932, 'GTCTA': 67384, 'GTCTT': 122087, 'GTCTC': 162458, 'GTCTG': 192530, 'GTCCA': 195525, 'GTCCT': 217798, 'GTCCC': 265182, 'GTCCG': 111145, 'GTCGA': 53295, 'GTCGT': 72651, 'GTCGC': 84029, 'GTCGG': 104064, 'GTGAA': 216313, 'GTGAT': 108549, 'GTGAC': 190973, 'GTGAG': 182777, 'GTGTA': 67295, 'GTGTT': 127257, 'GTGTC': 145230, 'GTGTG': 235600, 'GTGCA': 197827, 'GTGCT': 221420, 'GTGCC': 266768, 'GTGCG': 144675, 'GTGGA': 359574, 'GTGGT': 236281, 'GTGGC': 359471, 'GTGGG': 315568, 'GCAAA': 141118, 'GCAAT': 72256, 'GCAAC': 137456, 'GCAAG': 296913, 'GCATA': 30763, 'GCATT': 92955, 'GCATC': 202756, 'GCATG': 164703, 'GCACA': 167986, 'GCACT': 143334, 'GCACC': 293215, 'GCACG': 143651, 'GCAGA': 304816, 'GCAGT': 196189, 'GCAGC': 549504, 'GCAGG': 378013, 'GCTAA': 55442, 'GCTAT': 81374, 'GCTAC': 165027, 'GCTAG': 48515, 'GCTTA': 44323, 'GCTTT': 141314, 'GCTTC': 259544, 'GCTTG': 116437, 'GCTCA': 239185, 'GCTCT': 208303, 'GCTCC': 338529, 'GCTCG': 106705, 'GCTGA': 327430, 'GCTGT': 265436, 'GCTGC': 580939, 'GCTGG': 600820, 'GCCAA': 276061, 'GCCAT': 293157, 'GCCAC': 364782, 'GCCAG': 433202, 'GCCTA': 118368, 'GCCTT': 237074, 'GCCTC': 405662, 'GCCTG': 513790, 'GCCCA': 410218, 'GCCCT': 418433, 'GCCCC': 595149, 'GCCCG': 354296, 'GCCGA': 209351, 'GCCGT': 143864, 'GCCGC': 433425, 'GCCGG': 319574, 'GCGAA': 53138, 'GCGAT': 56599, 'GCGAC': 115549, 'GCGAG': 206201, 'GCGTA': 21647, 'GCGTT': 46518, 'GCGTC': 130444, 'GCGTG': 183956, 'GCGCA': 169033, 'GCGCT': 187702, 'GCGCC': 311844, 'GCGCG': 173468, 'GCGGA': 216394, 'GCGGT': 92930, 'GCGGC': 462331, 'GCGGG': 287370, 'GGAAA': 186446, 'GGAAT': 106241, 'GGAAC': 165899, 'GGAAG': 376073, 'GGATA': 56166, 'GGATT': 102895, 'GGATC': 130751, 'GGATG': 252449, 'GGACA': 308789, 'GGACT': 213070, 'GGACC': 365921, 'GGACG': 213606, 'GGAGA': 464730, 'GGAGT': 200652, 'GGAGC': 492138, 'GGAGG': 633534, 'GGTAA': 42254, 'GGTAT': 49757, 'GGTAC': 76249, 'GGTAG': 55033, 'GGTTA': 36270, 'GGTTT': 103826, 'GGTTC': 125085, 'GGTTG': 98422, 'GGTCA': 172418, 'GGTCT': 168091, 'GGTCC': 218903, 'GGTCG': 110984, 'GGTGA': 220143, 'GGTGT': 180899, 'GGTGC': 238553, 'GGTGG': 444650, 'GGCAA': 200427, 'GGCAT': 167135, 'GGCAC': 225153, 'GGCAG': 399607, 'GGCTA': 122158, 'GGCTT': 186483, 'GGCTC': 266902, 'GGCTG': 466486, 'GGCCA': 475470, 'GGCCT': 402548, 'GGCCC': 618063, 'GGCCG': 412618, 'GGCGA': 144793, 'GGCGT': 138699, 'GGCGC': 285136, 'GGCGG': 401441, 'GGGAA': 213980, 'GGGAT': 126328, 'GGGAC': 290344, 'GGGAG': 353831, 'GGGTA': 54152, 'GGGTT': 104621, 'GGGTC': 206998, 'GGGTG': 242393, 'GGGCA': 302330, 'GGGCT': 304231, 'GGGCC': 557841, 'GGGCG': 270642, 'GGGGA': 291355, 'GGGGT': 167010, 'GGGGC': 425688, 'GGGGG': 314576}, 'overrepresented_sequences': {}} {'total_reads': 2960672, 'total_bases': 171551948, 'q20_bases': 167133421, 'q30_bases': 161744951, 'total_cycles': 58, 'quality_curves': {'A': [37.1881, 37.1912, 37.1839, 37.2153, 37.1898, 37.2204, 37.2014, 37.1917, 37.1261, 37.0871, 37.1491, 37.1575, 37.1245, 37.0935, 37.0211, 37.0395, 37.0178, 36.9663, 37.0019, 37.0268, 37.0457, 37.0323, 37.0123, 36.899, 36.7821, 36.6279, 36.5361, 36.4742, 36.3757, 36.3546, 36.2997, 36.2658, 36.2083, 36.0404, 36.0287, 36.0075, 35.9422, 35.942, 35.8501, 35.797, 35.7247, 35.4202, 35.6371, 35.5366, 35.6092, 35.5715, 35.5395, 35.4789, 35.5578, 35.4751, 35.3278, 35.2358, 35.2119, 35.0966, 34.9677, 34.9236, 34.8577, 34.7229], 'T': [37.5294, 37.5057, 37.5131, 37.5071, 37.4789, 37.4651, 37.4354, 37.4396, 37.3628, 37.3439, 37.3538, 37.3601, 37.351, 37.3226, 37.3248, 37.3556, 37.354, 37.2878, 37.2813, 37.2781, 37.3113, 37.2922, 37.2965, 37.2346, 37.1759, 37.041, 36.952, 36.9107, 36.8208, 36.8015, 36.773, 36.7506, 36.6762, 36.5829, 36.5714, 36.524, 36.4962, 36.4683, 36.3853, 36.3234, 36.244, 35.964, 36.1411, 36.0877, 36.0877, 36.0914, 36.0437, 35.994, 36.0145, 35.9237, 35.7606, 35.6541, 35.603, 35.5376, 35.415, 35.345, 35.3049, 35.1401], 'C': [37.3554, 37.3985, 37.3553, 37.3562, 37.3703, 37.3508, 37.3649, 37.3695, 37.2901, 37.3138, 37.3288, 37.3485, 37.3297, 37.3507, 37.2918, 37.271, 37.2992, 37.3054, 37.2875, 37.2816, 37.2734, 37.2695, 37.2487, 37.1956, 37.0979, 37.0286, 36.9353, 36.8649, 36.8163, 36.7573, 36.7401, 36.7413, 36.6744, 36.6006, 36.5895, 36.5377, 36.5359, 36.501, 36.4476, 36.4071, 36.3767, 36.0789, 36.2417, 36.2577, 36.2604, 36.2493, 36.2419, 36.2158, 36.2027, 36.1629, 36.0612, 35.9762, 35.9133, 35.8237, 35.7462, 35.6661, 35.6414, 35.4677], 'G': [37.1193, 37.1529, 37.1211, 37.1499, 37.1314, 37.1253, 37.1253, 37.1545, 37.0732, 37.0825, 37.1259, 37.1411, 37.1048, 37.0814, 37.0238, 36.9939, 37.0107, 36.9822, 37.0071, 37.0438, 37.0177, 37.0192, 36.978, 36.8684, 36.7917, 36.679, 36.5766, 36.5308, 36.4704, 36.3948, 36.4031, 36.3934, 36.3312, 36.2379, 36.2179, 36.1969, 36.1791, 36.1634, 36.0724, 36.0423, 35.9741, 35.7529, 35.9086, 35.8571, 35.8818, 35.9194, 35.8764, 35.7783, 35.8156, 35.7797, 35.6399, 35.5217, 35.4849, 35.422, 35.3305, 35.2717, 35.2065, 35.0603], 'mean': [37.2956, 37.3088, 37.288, 37.2995, 37.2865, 37.2835, 37.2757, 37.2858, 37.2096, 37.2072, 37.238, 37.2511, 37.2263, 37.2118, 37.1646, 37.1604, 37.1686, 37.1374, 37.1458, 37.1592, 37.1602, 37.1524, 37.1315, 37.0476, 36.962, 36.8493, 36.7529, 36.699, 36.6279, 36.5793, 36.5604, 36.5464, 36.4808, 36.3785, 36.3662, 36.3284, 36.3052, 36.2835, 36.2026, 36.1599, 36.0999, 35.8252, 36.0015, 35.9619, 35.9813, 35.9819, 35.9527, 35.8903, 35.9187, 35.8604, 35.7241, 35.6244, 35.5809, 35.4971, 35.3964, 35.3332, 35.2824, 35.1286]}, 'content_curves': {'A': [0.199637, 0.193492, 0.200998, 0.201047, 0.197444, 0.203297, 0.199521, 0.196813, 0.20441, 0.197602, 0.200331, 0.205431, 0.202332, 0.197659, 0.204846, 0.200596, 0.198173, 0.203482, 0.199764, 0.195966, 0.202403, 0.199124, 0.196228, 0.200878, 0.19624, 0.194469, 0.200217, 0.195031, 0.194168, 0.198938, 0.195691, 0.194215, 0.199764, 0.197431, 0.193542, 0.199797, 0.196625, 0.195364, 0.201969, 0.196101, 0.194524, 0.200105, 0.196328, 0.192349, 0.198457, 0.198837, 0.193438, 0.200188, 0.194647, 0.195178, 0.197914, 0.194471, 0.194949, 0.198797, 0.196529, 0.194434, 0.198208, 0.195233], 'T': [0.245456, 0.229412, 0.235569, 0.227282, 0.222904, 0.230599, 0.226402, 0.225599, 0.231727, 0.231606, 0.223164, 0.228426, 0.229602, 0.219696, 0.224816, 0.224541, 0.219747, 0.225686, 0.225803, 0.222644, 0.2248, 0.226036, 0.22107, 0.224659, 0.223721, 0.220786, 0.225132, 0.225782, 0.221736, 0.226675, 0.225324, 0.221713, 0.227504, 0.227607, 0.224033, 0.22754, 0.225856, 0.220985, 0.222006, 0.223703, 0.220651, 0.225908, 0.225078, 0.222779, 0.224932, 0.227127, 0.221823, 0.226349, 0.226247, 0.222859, 0.221715, 0.225912, 0.218211, 0.220759, 0.222228, 0.217316, 0.22234, 0.219221], 'C': [0.262225, 0.2752, 0.265268, 0.26822, 0.27657, 0.268262, 0.271439, 0.277354, 0.269509, 0.273283, 0.278879, 0.273586, 0.271171, 0.278651, 0.276377, 0.274871, 0.280955, 0.27684, 0.277734, 0.279856, 0.276834, 0.27538, 0.282169, 0.277329, 0.281549, 0.286822, 0.278501, 0.279749, 0.283878, 0.276632, 0.279649, 0.283444, 0.278833, 0.27864, 0.284431, 0.278619, 0.283093, 0.284281, 0.281642, 0.281778, 0.285128, 0.279571, 0.281761, 0.287377, 0.283216, 0.280931, 0.285587, 0.281346, 0.279579, 0.28206, 0.283025, 0.282535, 0.288164, 0.28448, 0.284797, 0.287194, 0.283127, 0.286397], 'G': [0.292683, 0.301896, 0.298161, 0.30345, 0.303082, 0.297842, 0.302637, 0.300233, 0.294354, 0.297508, 0.297625, 0.292555, 0.296895, 0.303995, 0.29395, 0.299992, 0.301124, 0.293992, 0.296699, 0.301534, 0.295963, 0.29946, 0.300532, 0.297134, 0.29849, 0.297923, 0.29615, 0.299439, 0.300218, 0.297755, 0.299336, 0.300628, 0.293899, 0.296322, 0.297994, 0.294044, 0.294425, 0.299371, 0.294383, 0.298418, 0.299697, 0.294416, 0.296833, 0.297494, 0.293395, 0.293105, 0.299152, 0.292117, 0.299528, 0.299904, 0.297346, 0.297082, 0.298676, 0.295964, 0.296445, 0.301056, 0.296324, 0.299148], 'N': [0, 0, 4.05313e-06, 0, 0, 0, 0, 0, 0, 0, 6.75522e-07, 3.03985e-06, 0, 0, 1.01328e-05, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1.02151e-06], 'GC': [0.554907, 0.577096, 0.563429, 0.571671, 0.579652, 0.566104, 0.574076, 0.577587, 0.563863, 0.570791, 0.576504, 0.56614, 0.568066, 0.582646, 0.570328, 0.574863, 0.58208, 0.570832, 0.574433, 0.581389, 0.572797, 0.57484, 0.582701, 0.574463, 0.580039, 0.584745, 0.574651, 0.579188, 0.584096, 0.574387, 0.578985, 0.584072, 0.572732, 0.574962, 0.582425, 0.572663, 0.577518, 0.583652, 0.576025, 0.580196, 0.584825, 0.573987, 0.578594, 0.584872, 0.576611, 0.574035, 0.584739, 0.573463, 0.579106, 0.581964, 0.580371, 0.579617, 0.58684, 0.580444, 0.581242, 0.58825, 0.579451, 0.585545]}, 'kmer_count': {'AAAAA': 302688, 'AAAAT': 152179, 'AAAAC': 117309, 'AAAAG': 151784, 'AAATA': 131503, 'AAATT': 103363, 'AAATC': 95911, 'AAATG': 111412, 'AAACA': 142815, 'AAACT': 126717, 'AAACC': 104840, 'AAACG': 42477, 'AAAGA': 134029, 'AAAGT': 120127, 'AAAGC': 136313, 'AAAGG': 149771, 'AATAA': 103323, 'AATAT': 63806, 'AATAC': 53901, 'AATAG': 58386, 'AATTA': 57173, 'AATTT': 107352, 'AATTC': 86912, 'AATTG': 59469, 'AATCA': 77340, 'AATCT': 118290, 'AATCC': 93831, 'AATCG': 33942, 'AATGA': 94513, 'AATGT': 92767, 'AATGC': 92779, 'AATGG': 132824, 'AACAA': 99558, 'AACAT': 95150, 'AACAC': 110566, 'AACAG': 138700, 'AACTA': 34698, 'AACTT': 147476, 'AACTC': 129599, 'AACTG': 136297, 'AACCA': 95524, 'AACCT': 89427, 'AACCC': 99531, 'AACCG': 37713, 'AACGA': 22810, 'AACGT': 30028, 'AACGC': 39162, 'AACGG': 49773, 'AAGAA': 152607, 'AAGAT': 114238, 'AAGAC': 94923, 'AAGAG': 160744, 'AAGTA': 78337, 'AAGTT': 114699, 'AAGTC': 126496, 'AAGTG': 140691, 'AAGCA': 120873, 'AAGCT': 131667, 'AAGCC': 184703, 'AAGCG': 91455, 'AAGGA': 163377, 'AAGGT': 131812, 'AAGGC': 217933, 'AAGGG': 208146, 'ATAAA': 112140, 'ATAAT': 52180, 'ATAAC': 37443, 'ATAAG': 51462, 'ATATA': 49589, 'ATATT': 62011, 'ATATC': 41265, 'ATATG': 41667, 'ATACA': 77753, 'ATACT': 73542, 'ATACC': 42220, 'ATACG': 16723, 'ATAGA': 64497, 'ATAGT': 60334, 'ATAGC': 70669, 'ATAGG': 75572, 'ATTAA': 54289, 'ATTAT': 39061, 'ATTAC': 48181, 'ATTAG': 35569, 'ATTTA': 54951, 'ATTTT': 130571, 'ATTTC': 112477, 'ATTTG': 112528, 'ATTCA': 95484, 'ATTCT': 117595, 'ATTCC': 100967, 'ATTCG': 35956, 'ATTGA': 72495, 'ATTGT': 60392, 'ATTGC': 65893, 'ATTGG': 89245, 'ATCAA': 75113, 'ATCAT': 139739, 'ATCAC': 101040, 'ATCAG': 141946, 'ATCTA': 32777, 'ATCTT': 249836, 'ATCTC': 193952, 'ATCTG': 190090, 'ATCCA': 157252, 'ATCCT': 147717, 'ATCCC': 101114, 'ATCCG': 65960, 'ATCGA': 31834, 'ATCGT': 43716, 'ATCGC': 44829, 'ATCGG': 45298, 'ATGAA': 139308, 'ATGAT': 147189, 'ATGAC': 105011, 'ATGAG': 172802, 'ATGTA': 86009, 'ATGTT': 163731, 'ATGTC': 157498, 'ATGTG': 110376, 'ATGCA': 91192, 'ATGCT': 113400, 'ATGCC': 155520, 'ATGCG': 104613, 'ATGGA': 135048, 'ATGGT': 175008, 'ATGGC': 229456, 'ATGGG': 188610, 'ACAAA': 154665, 'ACAAT': 88757, 'ACAAC': 67754, 'ACAAG': 106681, 'ACATA': 71490, 'ACATT': 106736, 'ACATC': 122258, 'ACATG': 126576, 'ACACA': 167068, 'ACACT': 96108, 'ACACC': 157659, 'ACACG': 93113, 'ACAGA': 158849, 'ACAGT': 115780, 'ACAGC': 234204, 'ACAGG': 242477, 'ACTAA': 33271, 'ACTAT': 31383, 'ACTAC': 27722, 'ACTAG': 32807, 'ACTTA': 33514, 'ACTTT': 120946, 'ACTTC': 177078, 'ACTTG': 189955, 'ACTCA': 117902, 'ACTCT': 111599, 'ACTCC': 162050, 'ACTCG': 108557, 'ACTGA': 108383, 'ACTGT': 122737, 'ACTGC': 173347, 'ACTGG': 206924, 'ACCAA': 78433, 'ACCAT': 118808, 'ACCAC': 210233, 'ACCAG': 259983, 'ACCTA': 27826, 'ACCTT': 150560, 'ACCTC': 197254, 'ACCTG': 195179, 'ACCCA': 157153, 'ACCCT': 128839, 'ACCCC': 146081, 'ACCCG': 84773, 'ACCGA': 54292, 'ACCGT': 56938, 'ACCGC': 75519, 'ACCGG': 66423, 'ACGAA': 50529, 'ACGAT': 56054, 'ACGAC': 48871, 'ACGAG': 66032, 'ACGTA': 32970, 'ACGTT': 76092, 'ACGTC': 90681, 'ACGTG': 83333, 'ACGCA': 66402, 'ACGCT': 71551, 'ACGCC': 111112, 'ACGCG': 52669, 'ACGGA': 73993, 'ACGGT': 83049, 'ACGGC': 115682, 'ACGGG': 113595, 'AGAAA': 172181, 'AGAAT': 86156, 'AGAAC': 130541, 'AGAAG': 250603, 'AGATA': 72883, 'AGATT': 68288, 'AGATC': 106951, 'AGATG': 203619, 'AGACA': 128601, 'AGACT': 85282, 'AGACC': 143470, 'AGACG': 91856, 'AGAGA': 181223, 'AGAGT': 100809, 'AGAGC': 177898, 'AGAGG': 291259, 'AGTAA': 64711, 'AGTAT': 49820, 'AGTAC': 64062, 'AGTAG': 93548, 'AGTTA': 49463, 'AGTTT': 121790, 'AGTTC': 126078, 'AGTTG': 125683, 'AGTCA': 117924, 'AGTCT': 129009, 'AGTCC': 181171, 'AGTCG': 79242, 'AGTGA': 137158, 'AGTGT': 105562, 'AGTGC': 137261, 'AGTGG': 208680, 'AGCAA': 105977, 'AGCAT': 124822, 'AGCAC': 201797, 'AGCAG': 354298, 'AGCTA': 51416, 'AGCTT': 212437, 'AGCTC': 272535, 'AGCTG': 354755, 'AGCCA': 259703, 'AGCCT': 213864, 'AGCCC': 281413, 'AGCCG': 179803, 'AGCGA': 75022, 'AGCGT': 111394, 'AGCGC': 153279, 'AGCGG': 182338, 'AGGAA': 265630, 'AGGAT': 174697, 'AGGAC': 195560, 'AGGAG': 349867, 'AGGTA': 121287, 'AGGTT': 139604, 'AGGTC': 222601, 'AGGTG': 309338, 'AGGCA': 267514, 'AGGCT': 284232, 'AGGCC': 343691, 'AGGCG': 219261, 'AGGGA': 244800, 'AGGGT': 208084, 'AGGGC': 375417, 'AGGGG': 410201, 'TAAAA': 126731, 'TAAAT': 76428, 'TAAAC': 53273, 'TAAAG': 59887, 'TAATA': 40898, 'TAATT': 59545, 'TAATC': 53710, 'TAATG': 58850, 'TAACA': 58769, 'TAACT': 56290, 'TAACC': 35202, 'TAACG': 10337, 'TAAGA': 54420, 'TAAGT': 38967, 'TAAGC': 45382, 'TAAGG': 66388, 'TATAA': 40400, 'TATAT': 41036, 'TATAC': 27210, 'TATAG': 36966, 'TATTA': 31021, 'TATTT': 105734, 'TATTC': 58045, 'TATTG': 61674, 'TATCA': 50138, 'TATCT': 59925, 'TATCC': 50597, 'TATCG': 20199, 'TATGA': 41140, 'TATGT': 41650, 'TATGC': 30374, 'TATGG': 62852, 'TACAA': 71059, 'TACAT': 66550, 'TACAC': 71065, 'TACAG': 105003, 'TACTA': 18479, 'TACTT': 106241, 'TACTC': 78349, 'TACTG': 81807, 'TACCA': 58039, 'TACCT': 47131, 'TACCC': 52298, 'TACCG': 27671, 'TACGA': 16478, 'TACGT': 23556, 'TACGC': 18190, 'TACGG': 36874, 'TAGAA': 83935, 'TAGAT': 66176, 'TAGAC': 54773, 'TAGAG': 89754, 'TAGTA': 51599, 'TAGTT': 60929, 'TAGTC': 67162, 'TAGTG': 73190, 'TAGCA': 63606, 'TAGCT': 73968, 'TAGCC': 97314, 'TAGCG': 57231, 'TAGGA': 95254, 'TAGGT': 85231, 'TAGGC': 92918, 'TAGGG': 109962, 'TTAAA': 99333, 'TTAAT': 68640, 'TTAAC': 44807, 'TTAAG': 59035, 'TTATA': 41092, 'TTATT': 74406, 'TTATC': 50269, 'TTATG': 45849, 'TTACA': 73725, 'TTACT': 42403, 'TTACC': 36571, 'TTACG': 23499, 'TTAGA': 32873, 'TTAGT': 47663, 'TTAGC': 44521, 'TTAGG': 64349, 'TTTAA': 100414, 'TTTAT': 88266, 'TTTAC': 56733, 'TTTAG': 53348, 'TTTTA': 99462, 'TTTTT': 341877, 'TTTTC': 190570, 'TTTTG': 143677, 'TTTCA': 140853, 'TTTCT': 274608, 'TTTCC': 180423, 'TTTCG': 34735, 'TTTGA': 116670, 'TTTGT': 168450, 'TTTGC': 135496, 'TTTGG': 160747, 'TTCAA': 128957, 'TTCAT': 174666, 'TTCAC': 203278, 'TTCAG': 241809, 'TTCTA': 63370, 'TTCTT': 411282, 'TTCTC': 361772, 'TTCTG': 273631, 'TTCCA': 190995, 'TTCCT': 250638, 'TTCCC': 196451, 'TTCCG': 120310, 'TTCGA': 43961, 'TTCGT': 34876, 'TTCGC': 37610, 'TTCGG': 60442, 'TTGAA': 158666, 'TTGAT': 178440, 'TTGAC': 130797, 'TTGAG': 192687, 'TTGTA': 131943, 'TTGTT': 177541, 'TTGTC': 207445, 'TTGTG': 141803, 'TTGCA': 128202, 'TTGCT': 169423, 'TTGCC': 178388, 'TTGCG': 116249, 'TTGGA': 182229, 'TTGGT': 202841, 'TTGGC': 259941, 'TTGGG': 244890, 'TCAAA': 150091, 'TCAAT': 120211, 'TCAAC': 67678, 'TCAAG': 101756, 'TCATA': 88721, 'TCATT': 106123, 'TCATC': 276471, 'TCATG': 187589, 'TCACA': 180158, 'TCACT': 161494, 'TCACC': 199439, 'TCACG': 99119, 'TCAGA': 144348, 'TCAGT': 139571, 'TCAGC': 290295, 'TCAGG': 290043, 'TCTAA': 41420, 'TCTAT': 49412, 'TCTAC': 57229, 'TCTAG': 57394, 'TCTTA': 68238, 'TCTTT': 217036, 'TCTTC': 441677, 'TCTTG': 410665, 'TCTCA': 156971, 'TCTCT': 241556, 'TCTCC': 410560, 'TCTCG': 179866, 'TCTGA': 156744, 'TCTGT': 185407, 'TCTGC': 283794, 'TCTGG': 375184, 'TCCAA': 130392, 'TCCAT': 176401, 'TCCAC': 316090, 'TCCAG': 480372, 'TCCTA': 53086, 'TCCTT': 316330, 'TCCTC': 540260, 'TCCTG': 328007, 'TCCCA': 232467, 'TCCCT': 162725, 'TCCCC': 246243, 'TCCCG': 199782, 'TCCGA': 75864, 'TCCGT': 114239, 'TCCGC': 150206, 'TCCGG': 181568, 'TCGAA': 93903, 'TCGAT': 87534, 'TCGAC': 36246, 'TCGAG': 55419, 'TCGTA': 74290, 'TCGTT': 67177, 'TCGTC': 137679, 'TCGTG': 92902, 'TCGCA': 73246, 'TCGCT': 94477, 'TCGCC': 113891, 'TCGCG': 66103, 'TCGGA': 81549, 'TCGGT': 81444, 'TCGGC': 163892, 'TCGGG': 175277, 'TGAAA': 108648, 'TGAAT': 87012, 'TGAAC': 125497, 'TGAAG': 269127, 'TGATA': 54969, 'TGATT': 61323, 'TGATC': 143801, 'TGATG': 280589, 'TGACA': 132377, 'TGACT': 99593, 'TGACC': 161574, 'TGACG': 123519, 'TGAGA': 162182, 'TGAGT': 114271, 'TGAGC': 236585, 'TGAGG': 316158, 'TGTAA': 72926, 'TGTAT': 61789, 'TGTAC': 95486, 'TGTAG': 175880, 'TGTTA': 62137, 'TGTTT': 145879, 'TGTTC': 170594, 'TGTTG': 240163, 'TGTCA': 168177, 'TGTCT': 189628, 'TGTCC': 285671, 'TGTCG': 112561, 'TGTGA': 121242, 'TGTGT': 152012, 'TGTGC': 161305, 'TGTGG': 262330, 'TGCAA': 101303, 'TGCAT': 126007, 'TGCAC': 170307, 'TGCAG': 324918, 'TGCTA': 45818, 'TGCTT': 197215, 'TGCTC': 256904, 'TGCTG': 404463, 'TGCCA': 218975, 'TGCCT': 206753, 'TGCCC': 275758, 'TGCCG': 195259, 'TGCGA': 46577, 'TGCGT': 77578, 'TGCGC': 138437, 'TGCGG': 219044, 'TGGAA': 212557, 'TGGAT': 152554, 'TGGAC': 174717, 'TGGAG': 353017, 'TGGTA': 107809, 'TGGTT': 162665, 'TGGTC': 279057, 'TGGTG': 342736, 'TGGCA': 275799, 'TGGCT': 294319, 'TGGCC': 419132, 'TGGCG': 217659, 'TGGGA': 255432, 'TGGGT': 253597, 'TGGGC': 374928, 'TGGGG': 528667, 'CAAAA': 162002, 'CAAAT': 97783, 'CAAAC': 146772, 'CAAAG': 187148, 'CAATA': 53299, 'CAATT': 66835, 'CAATC': 85724, 'CAATG': 143328, 'CAACA': 110542, 'CAACT': 82606, 'CAACC': 80628, 'CAACG': 42748, 'CAAGA': 101469, 'CAAGT': 106416, 'CAAGC': 100236, 'CAAGG': 174622, 'CATAA': 60575, 'CATAT': 47346, 'CATAC': 83332, 'CATAG': 119812, 'CATTA': 56992, 'CATTT': 109638, 'CATTC': 132328, 'CATTG': 112157, 'CATCA': 204184, 'CATCT': 258331, 'CATCC': 214898, 'CATCG': 79587, 'CATGA': 183065, 'CATGT': 159621, 'CATGC': 153690, 'CATGG': 242832, 'CACAA': 156349, 'CACAT': 159084, 'CACAC': 202409, 'CACAG': 307119, 'CACTA': 43420, 'CACTT': 141460, 'CACTC': 160123, 'CACTG': 276226, 'CACCA': 333152, 'CACCT': 285343, 'CACCC': 212708, 'CACCG': 114308, 'CACGA': 107957, 'CACGT': 144314, 'CACGC': 150349, 'CACGG': 180450, 'CAGAA': 205368, 'CAGAT': 147005, 'CAGAC': 156430, 'CAGAG': 276074, 'CAGTA': 82699, 'CAGTT': 143282, 'CAGTC': 161826, 'CAGTG': 225922, 'CAGCA': 414418, 'CAGCT': 446228, 'CAGCC': 408004, 'CAGCG': 240195, 'CAGGA': 409134, 'CAGGT': 344830, 'CAGGC': 454192, 'CAGGG': 538630, 'CTAAA': 44999, 'CTAAT': 29446, 'CTAAC': 35538, 'CTAAG': 45361, 'CTATA': 20679, 'CTATT': 49122, 'CTATC': 40889, 'CTATG': 41076, 'CTACA': 53198, 'CTACT': 44946, 'CTACC': 43853, 'CTACG': 23466, 'CTAGA': 58814, 'CTAGT': 22120, 'CTAGC': 43017, 'CTAGG': 71050, 'CTTAA': 64291, 'CTTAT': 39619, 'CTTAC': 30050, 'CTTAG': 61325, 'CTTTA': 98759, 'CTTTT': 166198, 'CTTTC': 173911, 'CTTTG': 195185, 'CTTCA': 349901, 'CTTCT': 490932, 'CTTCC': 337575, 'CTTCG': 66462, 'CTTGA': 292503, 'CTTGT': 259893, 'CTTGC': 265426, 'CTTGG': 407332, 'CTCAA': 131108, 'CTCAT': 203121, 'CTCAC': 151934, 'CTCAG': 287083, 'CTCTA': 64873, 'CTCTT': 261140, 'CTCTC': 220531, 'CTCTG': 323055, 'CTCCA': 477248, 'CTCCT': 540143, 'CTCCC': 284855, 'CTCCG': 211760, 'CTCGA': 119878, 'CTCGT': 183687, 'CTCGC': 175860, 'CTCGG': 249121, 'CTGAA': 146655, 'CTGAT': 96125, 'CTGAC': 140713, 'CTGAG': 254009, 'CTGTA': 105040, 'CTGTT': 153199, 'CTGTC': 231214, 'CTGTG': 266597, 'CTGCA': 335160, 'CTGCT': 397666, 'CTGCC': 367467, 'CTGCG': 156804, 'CTGGA': 329678, 'CTGGT': 308379, 'CTGGC': 384448, 'CTGGG': 583607, 'CCAAA': 164200, 'CCAAT': 65326, 'CCAAC': 102175, 'CCAAG': 158448, 'CCATA': 82689, 'CCATT': 114633, 'CCATC': 198019, 'CCATG': 257261, 'CCACA': 277722, 'CCACT': 193163, 'CCACC': 383615, 'CCACG': 247573, 'CCAGA': 250591, 'CCAGT': 192860, 'CCAGC': 517379, 'CCAGG': 655503, 'CCTAA': 36212, 'CCTAT': 43300, 'CCTAC': 33632, 'CCTAG': 50803, 'CCTTA': 44329, 'CCTTT': 143867, 'CCTTC': 326245, 'CCTTG': 357955, 'CCTCA': 268167, 'CCTCT': 290992, 'CCTCC': 546118, 'CCTCG': 264923, 'CCTGA': 168091, 'CCTGT': 179412, 'CCTGC': 334179, 'CCTGG': 473123, 'CCCAA': 141265, 'CCCAT': 170383, 'CCCAC': 271685, 'CCCAG': 462100, 'CCCTA': 44276, 'CCCTT': 188602, 'CCCTC': 268694, 'CCCTG': 286861, 'CCCCA': 313075, 'CCCCT': 209774, 'CCCCC': 259895, 'CCCCG': 232404, 'CCCGA': 116515, 'CCCGT': 127901, 'CCCGC': 215545, 'CCCGG': 297669, 'CCGAA': 70208, 'CCGAT': 65828, 'CCGAC': 74068, 'CCGAG': 148272, 'CCGTA': 65548, 'CCGTT': 87441, 'CCGTC': 151376, 'CCGTG': 160318, 'CCGCA': 149093, 'CCGCT': 201615, 'CCGCC': 267764, 'CCGCG': 139460, 'CCGGA': 138089, 'CCGGT': 136556, 'CCGGC': 253101, 'CCGGG': 303939, 'CGAAA': 38649, 'CGAAT': 36092, 'CGAAC': 56711, 'CGAAG': 131608, 'CGATA': 18084, 'CGATT': 27581, 'CGATC': 73359, 'CGATG': 161559, 'CGACA': 51914, 'CGACT': 56691, 'CGACC': 67275, 'CGACG': 43375, 'CGAGA': 73375, 'CGAGT': 59600, 'CGAGC': 90815, 'CGAGG': 160654, 'CGTAA': 18601, 'CGTAT': 23026, 'CGTAC': 50275, 'CGTAG': 122919, 'CGTTA': 16512, 'CGTTT': 63678, 'CGTTC': 105373, 'CGTTG': 119247, 'CGTCA': 93454, 'CGTCT': 132473, 'CGTCC': 173457, 'CGTCG': 115688, 'CGTGA': 84116, 'CGTGT': 91400, 'CGTGC': 125234, 'CGTGG': 196561, 'CGCAA': 35761, 'CGCAT': 59181, 'CGCAC': 111350, 'CGCAG': 230339, 'CGCTA': 16884, 'CGCTT': 111508, 'CGCTC': 189767, 'CGCTG': 214600, 'CGCCA': 153711, 'CGCCT': 153260, 'CGCCC': 215764, 'CGCCG': 180703, 'CGCGA': 49952, 'CGCGT': 57874, 'CGCGC': 112435, 'CGCGG': 167387, 'CGGAA': 90417, 'CGGAT': 109631, 'CGGAC': 90576, 'CGGAG': 162032, 'CGGTA': 79040, 'CGGTT': 74023, 'CGGTC': 119997, 'CGGTG': 184522, 'CGGCA': 156999, 'CGGCT': 183479, 'CGGCC': 307180, 'CGGCG': 207117, 'CGGGA': 161867, 'CGGGT': 116194, 'CGGGC': 291607, 'CGGGG': 309348, 'GAAAA': 129471, 'GAAAT': 112345, 'GAAAC': 99043, 'GAAAG': 141083, 'GAATA': 53885, 'GAATT': 80277, 'GAATC': 86911, 'GAATG': 97810, 'GAACA': 132094, 'GAACT': 182408, 'GAACC': 102296, 'GAACG': 45754, 'GAAGA': 233232, 'GAAGT': 194393, 'GAAGC': 245785, 'GAAGG': 330878, 'GATAA': 48833, 'GATAT': 42516, 'GATAC': 45858, 'GATAG': 56157, 'GATTA': 30412, 'GATTT': 86657, 'GATTC': 72051, 'GATTG': 53966, 'GATCA': 124937, 'GATCT': 227009, 'GATCC': 113759, 'GATCG': 32113, 'GATGA': 243991, 'GATGT': 222140, 'GATGC': 186919, 'GATGG': 289361, 'GACAA': 89420, 'GACAT': 106843, 'GACAC': 130374, 'GACAG': 201403, 'GACTA': 28135, 'GACTT': 126889, 'GACTC': 134447, 'GACTG': 116876, 'GACCA': 180130, 'GACCT': 151221, 'GACCC': 155405, 'GACCG': 73117, 'GACGA': 74986, 'GACGT': 85659, 'GACGC': 94918, 'GACGG': 120092, 'GAGAA': 197655, 'GAGAT': 124537, 'GAGAC': 144319, 'GAGAG': 225286, 'GAGTA': 58316, 'GAGTT': 104395, 'GAGTC': 151085, 'GAGTG': 147264, 'GAGCA': 189474, 'GAGCT': 242755, 'GAGCC': 247969, 'GAGCG': 133985, 'GAGGA': 316596, 'GAGGT': 227561, 'GAGGC': 350986, 'GAGGG': 379384, 'GTAAA': 59349, 'GTAAT': 62265, 'GTAAC': 41821, 'GTAAG': 48653, 'GTATA': 33524, 'GTATT': 67958, 'GTATC': 47462, 'GTATG': 47041, 'GTACA': 108655, 'GTACT': 124054, 'GTACC': 62669, 'GTACG': 32004, 'GTAGA': 138912, 'GTAGT': 122119, 'GTAGC': 133338, 'GTAGG': 171814, 'GTTAA': 51377, 'GTTAT': 42833, 'GTTAC': 40859, 'GTTAG': 37494, 'GTTTA': 43847, 'GTTTT': 134267, 'GTTTC': 151685, 'GTTTG': 127577, 'GTTCA': 161403, 'GTTCT': 224209, 'GTTCC': 139306, 'GTTCG': 40521, 'GTTGA': 176837, 'GTTGT': 168165, 'GTTGC': 123657, 'GTTGG': 230882, 'GTCAA': 103769, 'GTCAT': 140145, 'GTCAC': 181712, 'GTCAG': 192876, 'GTCTA': 43561, 'GTCTT': 213314, 'GTCTC': 211934, 'GTCTG': 212559, 'GTCCA': 275975, 'GTCCT': 296967, 'GTCCC': 258759, 'GTCCG': 123209, 'GTCGA': 77570, 'GTCGT': 109825, 'GTCGC': 89933, 'GTCGG': 146812, 'GTGAA': 144069, 'GTGAT': 116227, 'GTGAC': 139497, 'GTGAG': 208738, 'GTGTA': 81918, 'GTGTT': 123346, 'GTGTC': 157591, 'GTGTG': 174092, 'GTGCA': 168413, 'GTGCT': 223325, 'GTGCC': 195945, 'GTGCG': 102737, 'GTGGA': 244131, 'GTGGT': 203991, 'GTGGC': 333348, 'GTGGG': 393950, 'GCAAA': 125732, 'GCAAT': 75745, 'GCAAC': 79907, 'GCAAG': 114689, 'GCATA': 70109, 'GCATT': 83530, 'GCATC': 162275, 'GCATG': 166648, 'GCACA': 201749, 'GCACT': 170874, 'GCACC': 207400, 'GCACG': 144746, 'GCAGA': 231423, 'GCAGT': 165955, 'GCAGC': 473097, 'GCAGG': 559197, 'GCTAA': 44075, 'GCTAT': 27734, 'GCTAC': 47263, 'GCTAG': 53965, 'GCTTA': 49625, 'GCTTT': 150318, 'GCTTC': 302313, 'GCTTG': 265365, 'GCTCA': 229986, 'GCTCT': 227798, 'GCTCC': 399374, 'GCTCG': 178812, 'GCTGA': 205572, 'GCTGT': 267693, 'GCTGC': 468056, 'GCTGG': 552293, 'GCCAA': 140523, 'GCCAT': 186140, 'GCCAC': 307165, 'GCCAG': 413678, 'GCCTA': 38985, 'GCCTT': 217963, 'GCCTC': 362872, 'GCCTG': 344619, 'GCCCA': 345207, 'GCCCT': 288115, 'GCCCC': 368004, 'GCCCG': 243390, 'GCCGA': 113063, 'GCCGT': 165638, 'GCCGC': 317876, 'GCCGG': 286211, 'GCGAA': 49420, 'GCGAT': 71193, 'GCGAC': 60798, 'GCGAG': 116109, 'GCGTA': 41895, 'GCGTT': 73159, 'GCGTC': 137240, 'GCGTG': 160974, 'GCGCA': 148374, 'GCGCT': 167812, 'GCGCC': 213289, 'GCGCG': 131653, 'GCGGA': 159370, 'GCGGT': 157965, 'GCGGC': 325190, 'GCGGG': 287421, 'GGAAA': 161992, 'GGAAT': 110081, 'GGAAC': 150530, 'GGAAG': 353860, 'GGATA': 47308, 'GGATT': 84392, 'GGATC': 173125, 'GGATG': 296372, 'GGACA': 213788, 'GGACT': 166308, 'GGACC': 188889, 'GGACG': 117342, 'GGAGA': 276372, 'GGAGT': 186846, 'GGAGC': 310733, 'GGAGG': 505954, 'GGTAA': 55535, 'GGTAT': 60518, 'GGTAC': 117566, 'GGTAG': 174223, 'GGTTA': 43395, 'GGTTT': 125477, 'GGTTC': 164892, 'GGTTG': 212896, 'GGTCA': 236830, 'GGTCT': 231634, 'GGTCC': 312813, 'GGTCG': 116045, 'GGTGA': 263968, 'GGTGT': 185351, 'GGTGC': 265838, 'GGTGG': 505228, 'GGCAA': 152820, 'GGCAT': 172385, 'GGCAC': 244320, 'GGCAG': 523929, 'GGCTA': 59311, 'GGCTT': 249740, 'GGCTC': 319049, 'GGCTG': 517145, 'GGCCA': 419075, 'GGCCT': 392897, 'GGCCC': 472932, 'GGCCG': 330454, 'GGCGA': 125911, 'GGCGT': 167162, 'GGCGC': 257892, 'GGCGG': 361902, 'GGGAA': 207073, 'GGGAT': 162722, 'GGGAC': 226828, 'GGGAG': 416557, 'GGGTA': 99477, 'GGGTT': 168335, 'GGGTC': 278549, 'GGGTG': 377349, 'GGGCA': 392478, 'GGGCT': 384424, 'GGGCC': 548947, 'GGGCG': 269441, 'GGGGA': 349693, 'GGGGT': 344550, 'GGGGC': 554139, 'GGGGG': 497893}, 'overrepresented_sequences': {}} {'total_reads': 3023055, 'total_bases': 190429253, 'q20_bases': 183633503, 'q30_bases': 176791370, 'total_cycles': 63, 'quality_curves': {'A': [37.136, 37.1391, 37.1267, 37.1581, 37.1306, 37.1631, 37.1423, 37.1287, 37.0624, 37.02, 37.0802, 37.0835, 37.0476, 37.0105, 36.935, 36.9525, 36.9271, 36.8714, 36.909, 36.9234, 36.9457, 36.9304, 36.903, 36.7949, 36.6759, 36.5161, 36.421, 36.3613, 36.2545, 36.2357, 36.1731, 36.1334, 36.0725, 35.8983, 35.882, 35.8566, 35.7928, 35.7884, 35.6987, 35.6424, 35.5607, 35.261, 35.4746, 35.3722, 35.4467, 35.4054, 35.3688, 35.3023, 35.3745, 35.2885, 35.1371, 35.0468, 35.0155, 34.9015, 34.7737, 34.7253, 34.6587, 34.5197, 34.4603, 34.1278, 33.5896, 33.1948, 24.2871], 'T': [37.4888, 37.4622, 37.4684, 37.4641, 37.4318, 37.4129, 37.3809, 37.3811, 37.303, 37.2795, 37.2863, 37.2926, 37.2803, 37.251, 37.2517, 37.2822, 37.2774, 37.2094, 37.2006, 37.1979, 37.2268, 37.2058, 37.2002, 37.1252, 37.0646, 36.9171, 36.8244, 36.7844, 36.6823, 36.6609, 36.6281, 36.5967, 36.5225, 36.4169, 36.3984, 36.3495, 36.3121, 36.2772, 36.1879, 36.1235, 36.0383, 35.7579, 35.9343, 35.8762, 35.8736, 35.8844, 35.8339, 35.7922, 35.8189, 35.7278, 35.5629, 35.4596, 35.4049, 35.3422, 35.2214, 35.1498, 35.1086, 34.9381, 34.8938, 34.5858, 34.0056, 33.7227, 25.1105], 'C': [37.2977, 37.3443, 37.2997, 37.3015, 37.3152, 37.2946, 37.3075, 37.314, 37.2285, 37.2529, 37.2674, 37.283, 37.2598, 37.2783, 37.2214, 37.1952, 37.2213, 37.225, 37.2084, 37.2007, 37.1906, 37.1848, 37.1642, 37.1114, 37.0104, 36.9388, 36.8428, 36.7661, 36.7158, 36.6535, 36.6282, 36.6305, 36.5555, 36.4822, 36.4674, 36.4056, 36.4024, 36.3667, 36.3069, 36.2651, 36.2337, 35.9378, 36.0969, 36.1099, 36.1141, 36.0968, 36.0877, 36.0587, 36.0382, 35.9992, 35.8962, 35.8022, 35.7413, 35.6449, 35.5608, 35.4764, 35.4438, 35.2704, 35.2011, 34.9863, 34.3915, 34.0276, 29.8825], 'G': [37.0498, 37.0834, 37.0505, 37.0797, 37.0637, 37.0519, 37.0512, 37.0785, 36.9963, 37.0018, 37.0422, 37.0556, 37.0072, 36.9816, 36.9177, 36.8843, 36.9022, 36.8713, 36.8924, 36.9279, 36.9015, 36.9043, 36.86, 36.7493, 36.6681, 36.5547, 36.4491, 36.4018, 36.3398, 36.2604, 36.2683, 36.2567, 36.1901, 36.0934, 36.0682, 36.046, 36.0224, 36.006, 35.9092, 35.8779, 35.8103, 35.5832, 35.7377, 35.6858, 35.704, 35.736, 35.6958, 35.5921, 35.6315, 35.5937, 35.4489, 35.3244, 35.2865, 35.2175, 35.1258, 35.0633, 34.9967, 34.8407, 34.7466, 34.4771, 33.8624, 33.4524, 28.2693], 'mean': [37.2398, 37.2529, 37.2303, 37.2423, 37.2285, 37.2229, 37.2136, 37.222, 37.1435, 37.1383, 37.167, 37.1775, 37.1465, 37.1291, 37.0798, 37.0727, 37.0791, 37.0455, 37.053, 37.0634, 37.0635, 37.0548, 37.0293, 36.9432, 36.8546, 36.7373, 36.6375, 36.5821, 36.5059, 36.4549, 36.4313, 36.4141, 36.3441, 36.2368, 36.2197, 36.1774, 36.1503, 36.126, 36.0406, 35.9958, 35.9327, 35.6574, 35.8315, 35.7898, 35.8071, 35.8052, 35.7751, 35.7105, 35.7376, 35.6783, 35.5384, 35.4359, 35.3905, 35.3035, 35.2018, 35.1348, 35.0812, 34.9224, 34.8539, 34.5755, 33.9913, 33.6246, 27.2383]}, 'content_curves': {'A': [0.199352, 0.193275, 0.200835, 0.200794, 0.197217, 0.203042, 0.199314, 0.196612, 0.204157, 0.197356, 0.200088, 0.205268, 0.202081, 0.197425, 0.204625, 0.200348, 0.197993, 0.203277, 0.199486, 0.195896, 0.202224, 0.198925, 0.196135, 0.200707, 0.196002, 0.194208, 0.199916, 0.194729, 0.193873, 0.198667, 0.195456, 0.193984, 0.199514, 0.197161, 0.193303, 0.199535, 0.196291, 0.194988, 0.201556, 0.195775, 0.194218, 0.199758, 0.19604, 0.192074, 0.198157, 0.198488, 0.193174, 0.199904, 0.19442, 0.195027, 0.197866, 0.194324, 0.194926, 0.199009, 0.196231, 0.194613, 0.197785, 0.195201, 0.193567, 0.199184, 0.195227, 0.193213, 0.19563], 'T': [0.245675, 0.229686, 0.235817, 0.227504, 0.223162, 0.230911, 0.226596, 0.225831, 0.231909, 0.231726, 0.223172, 0.228455, 0.229467, 0.21958, 0.224702, 0.224425, 0.219705, 0.225616, 0.225688, 0.222389, 0.224651, 0.225767, 0.220915, 0.224641, 0.223725, 0.220867, 0.225232, 0.225846, 0.22183, 0.226725, 0.225298, 0.221764, 0.227536, 0.227574, 0.22404, 0.227536, 0.225903, 0.22108, 0.222093, 0.223731, 0.220746, 0.225924, 0.225028, 0.222816, 0.225011, 0.227056, 0.221755, 0.226269, 0.226022, 0.22267, 0.221608, 0.225651, 0.218047, 0.220638, 0.221804, 0.216942, 0.222535, 0.218877, 0.216645, 0.219094, 0.222928, 0.21598, 0.21377], 'C': [0.262054, 0.27506, 0.265076, 0.267967, 0.276347, 0.26805, 0.271214, 0.277089, 0.269336, 0.273047, 0.27871, 0.273412, 0.271113, 0.278556, 0.276239, 0.274775, 0.280768, 0.276708, 0.277582, 0.279763, 0.276711, 0.27535, 0.281911, 0.277067, 0.281279, 0.286491, 0.278204, 0.279475, 0.283645, 0.2764, 0.279455, 0.283214, 0.278602, 0.278477, 0.284264, 0.278503, 0.28296, 0.284092, 0.281434, 0.281656, 0.284929, 0.279467, 0.281597, 0.287163, 0.282921, 0.280737, 0.285396, 0.28114, 0.279495, 0.2819, 0.282766, 0.28234, 0.287953, 0.284092, 0.284701, 0.286818, 0.282787, 0.286537, 0.287811, 0.283266, 0.284102, 0.286945, 0.288396], 'G': [0.292919, 0.301979, 0.298269, 0.303736, 0.303274, 0.297998, 0.302876, 0.300468, 0.294598, 0.297872, 0.298029, 0.292862, 0.297338, 0.304439, 0.294424, 0.300452, 0.301534, 0.294398, 0.297244, 0.301952, 0.296414, 0.299959, 0.301039, 0.297585, 0.298995, 0.298434, 0.296647, 0.29995, 0.300652, 0.298207, 0.299791, 0.301038, 0.294349, 0.296788, 0.298394, 0.294425, 0.294846, 0.29984, 0.294916, 0.298837, 0.300105, 0.294851, 0.297335, 0.297947, 0.293907, 0.293716, 0.299675, 0.292683, 0.300062, 0.300402, 0.297743, 0.297685, 0.299074, 0.29626, 0.297264, 0.301627, 0.296894, 0.299384, 0.301978, 0.298454, 0.297739, 0.303815, 0.300605], 'N': [0, 0, 3.96949e-06, 0, 0, 0, 0, 0, 0, 0, 6.61582e-07, 2.97712e-06, 0, 0, 9.92374e-06, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 2.31567e-06, 9.9244e-07, 0, 0, 3.30823e-06, 3.96991e-06, 0, 3.97002e-06, 0, 0, 1.62122e-05, 0, 0, 0, 0, 0, 0, 9.92967e-07, 0, 1.98593e-06, 4.30286e-06, 4.60075e-05, 0.00159768], 'GC': [0.554973, 0.577038, 0.563345, 0.571702, 0.579621, 0.566048, 0.57409, 0.577557, 0.563934, 0.570918, 0.576739, 0.566274, 0.568452, 0.582995, 0.570663, 0.575227, 0.582302, 0.571106, 0.574825, 0.581715, 0.573125, 0.575308, 0.58295, 0.574652, 0.580274, 0.584925, 0.574852, 0.579425, 0.584297, 0.574608, 0.579246, 0.584252, 0.57295, 0.575265, 0.582657, 0.572929, 0.577806, 0.583932, 0.57635, 0.580493, 0.585034, 0.574317, 0.578932, 0.58511, 0.576829, 0.574453, 0.585071, 0.573823, 0.579557, 0.582302, 0.58051, 0.580025, 0.587027, 0.580353, 0.581965, 0.588445, 0.57968, 0.585921, 0.589788, 0.58172, 0.581841, 0.59076, 0.589002]}, 'kmer_count': {'AAAAA': 360232, 'AAAAT': 168313, 'AAAAC': 131438, 'AAAAG': 170790, 'AAATA': 145461, 'AAATT': 114484, 'AAATC': 105659, 'AAATG': 121955, 'AAACA': 158771, 'AAACT': 140495, 'AAACC': 116149, 'AAACG': 46518, 'AAAGA': 150500, 'AAAGT': 134561, 'AAAGC': 151876, 'AAAGG': 166996, 'AATAA': 113681, 'AATAT': 69718, 'AATAC': 61124, 'AATAG': 66940, 'AATTA': 62553, 'AATTT': 119292, 'AATTC': 96551, 'AATTG': 64872, 'AATCA': 84558, 'AATCT': 131923, 'AATCC': 102870, 'AATCG': 39222, 'AATGA': 104113, 'AATGT': 101939, 'AATGC': 102111, 'AATGG': 145765, 'AACAA': 109949, 'AACAT': 105451, 'AACAC': 122413, 'AACAG': 155797, 'AACTA': 38284, 'AACTT': 162042, 'AACTC': 143055, 'AACTG': 152024, 'AACCA': 106004, 'AACCT': 98426, 'AACCC': 111169, 'AACCG': 42027, 'AACGA': 25349, 'AACGT': 32984, 'AACGC': 43361, 'AACGG': 54533, 'AAGAA': 169694, 'AAGAT': 126587, 'AAGAC': 107701, 'AAGAG': 177980, 'AAGTA': 87026, 'AAGTT': 129299, 'AAGTC': 140398, 'AAGTG': 153872, 'AAGCA': 133964, 'AAGCT': 145850, 'AAGCC': 202480, 'AAGCG': 101478, 'AAGGA': 180809, 'AAGGT': 147607, 'AAGGC': 243289, 'AAGGG': 235600, 'ATAAA': 124916, 'ATAAT': 57925, 'ATAAC': 41785, 'ATAAG': 56488, 'ATATA': 56318, 'ATATT': 67845, 'ATATC': 46005, 'ATATG': 45925, 'ATACA': 86555, 'ATACT': 82720, 'ATACC': 48366, 'ATACG': 18571, 'ATAGA': 72302, 'ATAGT': 66597, 'ATAGC': 79026, 'ATAGG': 85337, 'ATTAA': 60392, 'ATTAT': 42856, 'ATTAC': 52688, 'ATTAG': 39947, 'ATTTA': 60529, 'ATTTT': 146225, 'ATTTC': 125280, 'ATTTG': 123177, 'ATTCA': 105218, 'ATTCT': 130782, 'ATTCC': 112329, 'ATTCG': 39937, 'ATTGA': 79694, 'ATTGT': 66299, 'ATTGC': 73032, 'ATTGG': 98536, 'ATCAA': 83099, 'ATCAT': 154983, 'ATCAC': 111326, 'ATCAG': 155518, 'ATCTA': 35886, 'ATCTT': 273683, 'ATCTC': 214995, 'ATCTG': 208644, 'ATCCA': 172668, 'ATCCT': 164731, 'ATCCC': 114977, 'ATCCG': 72994, 'ATCGA': 35918, 'ATCGT': 49175, 'ATCGC': 51154, 'ATCGG': 51685, 'ATGAA': 153153, 'ATGAT': 162379, 'ATGAC': 116909, 'ATGAG': 189838, 'ATGTA': 94137, 'ATGTT': 181150, 'ATGTC': 174792, 'ATGTG': 122157, 'ATGCA': 102083, 'ATGCT': 125625, 'ATGCC': 172292, 'ATGCG': 115393, 'ATGGA': 149142, 'ATGGT': 192298, 'ATGGC': 258604, 'ATGGG': 211408, 'ACAAA': 171104, 'ACAAT': 96934, 'ACAAC': 74818, 'ACAAG': 118302, 'ACATA': 80285, 'ACATT': 118229, 'ACATC': 135409, 'ACATG': 142883, 'ACACA': 185583, 'ACACT': 106079, 'ACACC': 175284, 'ACACG': 104203, 'ACAGA': 179189, 'ACAGT': 130159, 'ACAGC': 261685, 'ACAGG': 268137, 'ACTAA': 36672, 'ACTAT': 34818, 'ACTAC': 31293, 'ACTAG': 36322, 'ACTTA': 36985, 'ACTTT': 134780, 'ACTTC': 195587, 'ACTTG': 209932, 'ACTCA': 133883, 'ACTCT': 123081, 'ACTCC': 183053, 'ACTCG': 122118, 'ACTGA': 120557, 'ACTGT': 135525, 'ACTGC': 193351, 'ACTGG': 229863, 'ACCAA': 86380, 'ACCAT': 131615, 'ACCAC': 233243, 'ACCAG': 288995, 'ACCTA': 30213, 'ACCTT': 167158, 'ACCTC': 219311, 'ACCTG': 216056, 'ACCCA': 174775, 'ACCCT': 146140, 'ACCCC': 166325, 'ACCCG': 96758, 'ACCGA': 59348, 'ACCGT': 62947, 'ACCGC': 83745, 'ACCGG': 75552, 'ACGAA': 58100, 'ACGAT': 62814, 'ACGAC': 55410, 'ACGAG': 75770, 'ACGTA': 36335, 'ACGTT': 85331, 'ACGTC': 101072, 'ACGTG': 92071, 'ACGCA': 76007, 'ACGCT': 79167, 'ACGCC': 124001, 'ACGCG': 59773, 'ACGGA': 82516, 'ACGGT': 91575, 'ACGGC': 129287, 'ACGGG': 126593, 'AGAAA': 191199, 'AGAAT': 95376, 'AGAAC': 145078, 'AGAAG': 278444, 'AGATA': 81396, 'AGATT': 76189, 'AGATC': 120536, 'AGATG': 227864, 'AGACA': 144366, 'AGACT': 95913, 'AGACC': 163120, 'AGACG': 103908, 'AGAGA': 203290, 'AGAGT': 112198, 'AGAGC': 199126, 'AGAGG': 324453, 'AGTAA': 71351, 'AGTAT': 55026, 'AGTAC': 71787, 'AGTAG': 104027, 'AGTTA': 54783, 'AGTTT': 133672, 'AGTTC': 142141, 'AGTTG': 140528, 'AGTCA': 129162, 'AGTCT': 142958, 'AGTCC': 201713, 'AGTCG': 89191, 'AGTGA': 151215, 'AGTGT': 115777, 'AGTGC': 152559, 'AGTGG': 234959, 'AGCAA': 117856, 'AGCAT': 139223, 'AGCAC': 225777, 'AGCAG': 396995, 'AGCTA': 59270, 'AGCTT': 238234, 'AGCTC': 305624, 'AGCTG': 396359, 'AGCCA': 289963, 'AGCCT': 237060, 'AGCCC': 315020, 'AGCCG': 201037, 'AGCGA': 83393, 'AGCGT': 122668, 'AGCGC': 172541, 'AGCGG': 205610, 'AGGAA': 292908, 'AGGAT': 193742, 'AGGAC': 217894, 'AGGAG': 391440, 'AGGTA': 135359, 'AGGTT': 154294, 'AGGTC': 246750, 'AGGTG': 344067, 'AGGCA': 299480, 'AGGCT': 317776, 'AGGCC': 385942, 'AGGCG': 244142, 'AGGGA': 273612, 'AGGGT': 235310, 'AGGGC': 419754, 'AGGGG': 464742, 'TAAAA': 144039, 'TAAAT': 84910, 'TAAAC': 58481, 'TAAAG': 66431, 'TAATA': 46032, 'TAATT': 65548, 'TAATC': 59106, 'TAATG': 64171, 'TAACA': 64484, 'TAACT': 61474, 'TAACC': 39023, 'TAACG': 11438, 'TAAGA': 60033, 'TAAGT': 42725, 'TAAGC': 49894, 'TAAGG': 73017, 'TATAA': 45687, 'TATAT': 45908, 'TATAC': 29975, 'TATAG': 40661, 'TATTA': 34975, 'TATTT': 116415, 'TATTC': 63619, 'TATTG': 66915, 'TATCA': 55411, 'TATCT': 64544, 'TATCC': 56017, 'TATCG': 23325, 'TATGA': 45117, 'TATGT': 46940, 'TATGC': 33916, 'TATGG': 69238, 'TACAA': 78378, 'TACAT': 74159, 'TACAC': 78258, 'TACAG': 115162, 'TACTA': 20768, 'TACTT': 118690, 'TACTC': 87094, 'TACTG': 91933, 'TACCA': 64160, 'TACCT': 52931, 'TACCC': 58992, 'TACCG': 30393, 'TACGA': 18744, 'TACGT': 26741, 'TACGC': 20245, 'TACGG': 41565, 'TAGAA': 92441, 'TAGAT': 74736, 'TAGAC': 60998, 'TAGAG': 99509, 'TAGTA': 57681, 'TAGTT': 67090, 'TAGTC': 74654, 'TAGTG': 82132, 'TAGCA': 70663, 'TAGCT': 82839, 'TAGCC': 109022, 'TAGCG': 63450, 'TAGGA': 105791, 'TAGGT': 94170, 'TAGGC': 106337, 'TAGGG': 123805, 'TTAAA': 112795, 'TTAAT': 75763, 'TTAAC': 49348, 'TTAAG': 64413, 'TTATA': 46176, 'TTATT': 83200, 'TTATC': 55836, 'TTATG': 49805, 'TTACA': 81185, 'TTACT': 47639, 'TTACC': 39963, 'TTACG': 26118, 'TTAGA': 37363, 'TTAGT': 53132, 'TTAGC': 49298, 'TTAGG': 71549, 'TTTAA': 112099, 'TTTAT': 98326, 'TTTAC': 62627, 'TTTAG': 60746, 'TTTTA': 113634, 'TTTTT': 454852, 'TTTTC': 211180, 'TTTTG': 161829, 'TTTCA': 156032, 'TTTCT': 304761, 'TTTCC': 198023, 'TTTCG': 39489, 'TTTGA': 129137, 'TTTGT': 185988, 'TTTGC': 151005, 'TTTGG': 178179, 'TTCAA': 140639, 'TTCAT': 194309, 'TTCAC': 225384, 'TTCAG': 270779, 'TTCTA': 68471, 'TTCTT': 455415, 'TTCTC': 403136, 'TTCTG': 305274, 'TTCCA': 210241, 'TTCCT': 278065, 'TTCCC': 217580, 'TTCCG': 137774, 'TTCGA': 48624, 'TTCGT': 39493, 'TTCGC': 42181, 'TTCGG': 68595, 'TTGAA': 176511, 'TTGAT': 194717, 'TTGAC': 142383, 'TTGAG': 213985, 'TTGTA': 147294, 'TTGTT': 197363, 'TTGTC': 229509, 'TTGTG': 156596, 'TTGCA': 141582, 'TTGCT': 187403, 'TTGCC': 198900, 'TTGCG': 129856, 'TTGGA': 202440, 'TTGGT': 225590, 'TTGGC': 287220, 'TTGGG': 272434, 'TCAAA': 166409, 'TCAAT': 132324, 'TCAAC': 74851, 'TCAAG': 112197, 'TCATA': 98387, 'TCATT': 117338, 'TCATC': 304832, 'TCATG': 208716, 'TCACA': 201106, 'TCACT': 177711, 'TCACC': 222445, 'TCACG': 109552, 'TCAGA': 160907, 'TCAGT': 154241, 'TCAGC': 326309, 'TCAGG': 325264, 'TCTAA': 44885, 'TCTAT': 53327, 'TCTAC': 64228, 'TCTAG': 63177, 'TCTTA': 76277, 'TCTTT': 243295, 'TCTTC': 488314, 'TCTTG': 451724, 'TCTCA': 174562, 'TCTCT': 268711, 'TCTCC': 457483, 'TCTCG': 201378, 'TCTGA': 174358, 'TCTGT': 206103, 'TCTGC': 317305, 'TCTGG': 417747, 'TCCAA': 142574, 'TCCAT': 195097, 'TCCAC': 349991, 'TCCAG': 534425, 'TCCTA': 58594, 'TCCTT': 348292, 'TCCTC': 601648, 'TCCTG': 364119, 'TCCCA': 259554, 'TCCCT': 182907, 'TCCCC': 278556, 'TCCCG': 225036, 'TCCGA': 86850, 'TCCGT': 127051, 'TCCGC': 170539, 'TCCGG': 206593, 'TCGAA': 103242, 'TCGAT': 98457, 'TCGAC': 41357, 'TCGAG': 62317, 'TCGTA': 82930, 'TCGTT': 74592, 'TCGTC': 155614, 'TCGTG': 102647, 'TCGCA': 81952, 'TCGCT': 106697, 'TCGCC': 129592, 'TCGCG': 74552, 'TCGGA': 91890, 'TCGGT': 90914, 'TCGGC': 185388, 'TCGGG': 197309, 'TGAAA': 119196, 'TGAAT': 96594, 'TGAAC': 140027, 'TGAAG': 298253, 'TGATA': 60748, 'TGATT': 67840, 'TGATC': 158050, 'TGATG': 307747, 'TGACA': 147146, 'TGACT': 109444, 'TGACC': 177999, 'TGACG': 136088, 'TGAGA': 178914, 'TGAGT': 126112, 'TGAGC': 264656, 'TGAGG': 353857, 'TGTAA': 79799, 'TGTAT': 68509, 'TGTAC': 106291, 'TGTAG': 195001, 'TGTTA': 67660, 'TGTTT': 161098, 'TGTTC': 189619, 'TGTTG': 265950, 'TGTCA': 185382, 'TGTCT': 210199, 'TGTCC': 316854, 'TGTCG': 127211, 'TGTGA': 133454, 'TGTGT': 170174, 'TGTGC': 177757, 'TGTGG': 289322, 'TGCAA': 112198, 'TGCAT': 139352, 'TGCAC': 190438, 'TGCAG': 362130, 'TGCTA': 50944, 'TGCTT': 219003, 'TGCTC': 284675, 'TGCTG': 450892, 'TGCCA': 244646, 'TGCCT': 229274, 'TGCCC': 306656, 'TGCCG': 218905, 'TGCGA': 52465, 'TGCGT': 87935, 'TGCGC': 155597, 'TGCGG': 242029, 'TGGAA': 234400, 'TGGAT': 170439, 'TGGAC': 194381, 'TGGAG': 395749, 'TGGTA': 120845, 'TGGTT': 178123, 'TGGTC': 312697, 'TGGTG': 379575, 'TGGCA': 307294, 'TGGCT': 325671, 'TGGCC': 466583, 'TGGCG': 245075, 'TGGGA': 283447, 'TGGGT': 281268, 'TGGGC': 420033, 'TGGGG': 593132, 'CAAAA': 179126, 'CAAAT': 107745, 'CAAAC': 162405, 'CAAAG': 210529, 'CAATA': 59187, 'CAATT': 73677, 'CAATC': 94640, 'CAATG': 157194, 'CAACA': 122528, 'CAACT': 90910, 'CAACC': 90026, 'CAACG': 47354, 'CAAGA': 113548, 'CAAGT': 116570, 'CAAGC': 110966, 'CAAGG': 195359, 'CATAA': 67074, 'CATAT': 52550, 'CATAC': 93748, 'CATAG': 134059, 'CATTA': 62511, 'CATTT': 122703, 'CATTC': 147273, 'CATTG': 124987, 'CATCA': 226553, 'CATCT': 285153, 'CATCC': 240588, 'CATCG': 89032, 'CATGA': 202759, 'CATGT': 178361, 'CATGC': 170548, 'CATGG': 270986, 'CACAA': 173100, 'CACAT': 178401, 'CACAC': 224759, 'CACAG': 342706, 'CACTA': 48728, 'CACTT': 156094, 'CACTC': 181618, 'CACTG': 304143, 'CACCA': 370928, 'CACCT': 314462, 'CACCC': 239418, 'CACCG': 127765, 'CACGA': 120975, 'CACGT': 160211, 'CACGC': 170024, 'CACGG': 201326, 'CAGAA': 227114, 'CAGAT': 165165, 'CAGAC': 176822, 'CAGAG': 310421, 'CAGTA': 94001, 'CAGTT': 157608, 'CAGTC': 179012, 'CAGTG': 254428, 'CAGCA': 461342, 'CAGCT': 501846, 'CAGCC': 457308, 'CAGCG': 270309, 'CAGGA': 455244, 'CAGGT': 381074, 'CAGGC': 507604, 'CAGGG': 603458, 'CTAAA': 49709, 'CTAAT': 32045, 'CTAAC': 39002, 'CTAAG': 49858, 'CTATA': 22936, 'CTATT': 54075, 'CTATC': 44628, 'CTATG': 45117, 'CTACA': 59077, 'CTACT': 50489, 'CTACC': 50027, 'CTACG': 26520, 'CTAGA': 64890, 'CTAGT': 24709, 'CTAGC': 48202, 'CTAGG': 78309, 'CTTAA': 71348, 'CTTAT': 45542, 'CTTAC': 34190, 'CTTAG': 68202, 'CTTTA': 108269, 'CTTTT': 185896, 'CTTTC': 193187, 'CTTTG': 215760, 'CTTCA': 389870, 'CTTCT': 544003, 'CTTCC': 376362, 'CTTCG': 74665, 'CTTGA': 320643, 'CTTGT': 290404, 'CTTGC': 295714, 'CTTGG': 454387, 'CTCAA': 145689, 'CTCAT': 223427, 'CTCAC': 171107, 'CTCAG': 321360, 'CTCTA': 71536, 'CTCTT': 290519, 'CTCTC': 245547, 'CTCTG': 360148, 'CTCCA': 529452, 'CTCCT': 598135, 'CTCCC': 324483, 'CTCCG': 243233, 'CTCGA': 133709, 'CTCGT': 204662, 'CTCGC': 200257, 'CTCGG': 281002, 'CTGAA': 163601, 'CTGAT': 106904, 'CTGAC': 156998, 'CTGAG': 286296, 'CTGTA': 116317, 'CTGTT': 168276, 'CTGTC': 258539, 'CTGTG': 294710, 'CTGCA': 373645, 'CTGCT': 445329, 'CTGCC': 410046, 'CTGCG': 175973, 'CTGGA': 367465, 'CTGGT': 342759, 'CTGGC': 429090, 'CTGGG': 653564, 'CCAAA': 184117, 'CCAAT': 72107, 'CCAAC': 113842, 'CCAAG': 176471, 'CCATA': 91239, 'CCATT': 128036, 'CCATC': 220142, 'CCATG': 287079, 'CCACA': 307621, 'CCACT': 217665, 'CCACC': 426973, 'CCACG': 276367, 'CCAGA': 281168, 'CCAGT': 214638, 'CCAGC': 578048, 'CCAGG': 730906, 'CCTAA': 39795, 'CCTAT': 47320, 'CCTAC': 38287, 'CCTAG': 56176, 'CCTTA': 50011, 'CCTTT': 159367, 'CCTTC': 361958, 'CCTTG': 398924, 'CCTCA': 298596, 'CCTCT': 322645, 'CCTCC': 610457, 'CCTCG': 296593, 'CCTGA': 188905, 'CCTGT': 199055, 'CCTGC': 371194, 'CCTGG': 528918, 'CCCAA': 160332, 'CCCAT': 190390, 'CCCAC': 303257, 'CCCAG': 516184, 'CCCTA': 49204, 'CCCTT': 209969, 'CCCTC': 305023, 'CCCTG': 322163, 'CCCCA': 351622, 'CCCCT': 236202, 'CCCCC': 301970, 'CCCCG': 262856, 'CCCGA': 132227, 'CCCGT': 142267, 'CCCGC': 243189, 'CCCGG': 339631, 'CCGAA': 80053, 'CCGAT': 72361, 'CCGAC': 83579, 'CCGAG': 168654, 'CCGTA': 71944, 'CCGTT': 97370, 'CCGTC': 168713, 'CCGTG': 179556, 'CCGCA': 167967, 'CCGCT': 226774, 'CCGCC': 304456, 'CCGCG': 157875, 'CCGGA': 156615, 'CCGGT': 152779, 'CCGGC': 289666, 'CCGGG': 344823, 'CGAAA': 43180, 'CGAAT': 42179, 'CGAAC': 62775, 'CGAAG': 147034, 'CGATA': 20057, 'CGATT': 30195, 'CGATC': 82342, 'CGATG': 181059, 'CGACA': 58428, 'CGACT': 64078, 'CGACC': 76640, 'CGACG': 50325, 'CGAGA': 83328, 'CGAGT': 66867, 'CGAGC': 101007, 'CGAGG': 182211, 'CGTAA': 20239, 'CGTAT': 25816, 'CGTAC': 55498, 'CGTAG': 137076, 'CGTTA': 18411, 'CGTTT': 71294, 'CGTTC': 116554, 'CGTTG': 133817, 'CGTCA': 104295, 'CGTCT': 147768, 'CGTCC': 194928, 'CGTCG': 129125, 'CGTGA': 92499, 'CGTGT': 101609, 'CGTGC': 139988, 'CGTGG': 219365, 'CGCAA': 40132, 'CGCAT': 65008, 'CGCAC': 123745, 'CGCAG': 260420, 'CGCTA': 18997, 'CGCTT': 124032, 'CGCTC': 215606, 'CGCTG': 242224, 'CGCCA': 172641, 'CGCCT': 170919, 'CGCCC': 243936, 'CGCCG': 207972, 'CGCGA': 55939, 'CGCGT': 65858, 'CGCGC': 127494, 'CGCGG': 189390, 'CGGAA': 101783, 'CGGAT': 121336, 'CGGAC': 102272, 'CGGAG': 182804, 'CGGTA': 89476, 'CGGTT': 81043, 'CGGTC': 135912, 'CGGTG': 205923, 'CGGCA': 176305, 'CGGCT': 205776, 'CGGCC': 350132, 'CGGCG': 235265, 'CGGGA': 181847, 'CGGGT': 130390, 'CGGGC': 327987, 'CGGGG': 352640, 'GAAAA': 146780, 'GAAAT': 123247, 'GAAAC': 108538, 'GAAAG': 156242, 'GAATA': 59577, 'GAATT': 89106, 'GAATC': 97707, 'GAATG': 107760, 'GAACA': 147370, 'GAACT': 201680, 'GAACC': 113735, 'GAACG': 50967, 'GAAGA': 258351, 'GAAGT': 217636, 'GAAGC': 270428, 'GAAGG': 372805, 'GATAA': 54539, 'GATAT': 48112, 'GATAC': 51291, 'GATAG': 62173, 'GATTA': 34094, 'GATTT': 96056, 'GATTC': 79711, 'GATTG': 60057, 'GATCA': 137319, 'GATCT': 250089, 'GATCC': 127587, 'GATCG': 37032, 'GATGA': 268893, 'GATGT': 244009, 'GATGC': 208038, 'GATGG': 326046, 'GACAA': 99462, 'GACAT': 118000, 'GACAC': 145482, 'GACAG': 225807, 'GACTA': 30938, 'GACTT': 141424, 'GACTC': 151118, 'GACTG': 131344, 'GACCA': 201241, 'GACCT': 167641, 'GACCC': 177971, 'GACCG': 81482, 'GACGA': 87302, 'GACGT': 95043, 'GACGC': 106615, 'GACGG': 133240, 'GAGAA': 220942, 'GAGAT': 140572, 'GAGAC': 163513, 'GAGAG': 251125, 'GAGTA': 63716, 'GAGTT': 116833, 'GAGTC': 168803, 'GAGTG': 163536, 'GAGCA': 214377, 'GAGCT': 271401, 'GAGCC': 276420, 'GAGCG': 149267, 'GAGGA': 351377, 'GAGGT': 254747, 'GAGGC': 393703, 'GAGGG': 431001, 'GTAAA': 65293, 'GTAAT': 67858, 'GTAAC': 45251, 'GTAAG': 54423, 'GTATA': 36516, 'GTATT': 74137, 'GTATC': 51378, 'GTATG': 54045, 'GTACA': 119077, 'GTACT': 138996, 'GTACC': 69220, 'GTACG': 36448, 'GTAGA': 152967, 'GTAGT': 137016, 'GTAGC': 148381, 'GTAGG': 194062, 'GTTAA': 56921, 'GTTAT': 46651, 'GTTAC': 44788, 'GTTAG': 41034, 'GTTTA': 48600, 'GTTTT': 150326, 'GTTTC': 166414, 'GTTTG': 141414, 'GTTCA': 179799, 'GTTCT': 249190, 'GTTCC': 157493, 'GTTCG': 45283, 'GTTGA': 195546, 'GTTGT': 186171, 'GTTGC': 137829, 'GTTGG': 256319, 'GTCAA': 115027, 'GTCAT': 154154, 'GTCAC': 201727, 'GTCAG': 216362, 'GTCTA': 48841, 'GTCTT': 235510, 'GTCTC': 235570, 'GTCTG': 238520, 'GTCCA': 306272, 'GTCCT': 328653, 'GTCCC': 290836, 'GTCCG': 138849, 'GTCGA': 88022, 'GTCGT': 123006, 'GTCGC': 100173, 'GTCGG': 164178, 'GTGAA': 159157, 'GTGAT': 127505, 'GTGAC': 152869, 'GTGAG': 230027, 'GTGTA': 91009, 'GTGTT': 136549, 'GTGTC': 175129, 'GTGTG': 192111, 'GTGCA': 186424, 'GTGCT': 246890, 'GTGCC': 218380, 'GTGCG': 115195, 'GTGGA': 272528, 'GTGGT': 228040, 'GTGGC': 369332, 'GTGGG': 438385, 'GCAAA': 138594, 'GCAAT': 83606, 'GCAAC': 88187, 'GCAAG': 129363, 'GCATA': 78107, 'GCATT': 92977, 'GCATC': 181015, 'GCATG': 184632, 'GCACA': 225643, 'GCACT': 188679, 'GCACC': 231580, 'GCACG': 163959, 'GCAGA': 258924, 'GCAGT': 186861, 'GCAGC': 532391, 'GCAGG': 625661, 'GCTAA': 48935, 'GCTAT': 31017, 'GCTAC': 54359, 'GCTAG': 60567, 'GCTTA': 56073, 'GCTTT': 165858, 'GCTTC': 338402, 'GCTTG': 296309, 'GCTCA': 256636, 'GCTCT': 255176, 'GCTCC': 450096, 'GCTCG': 201537, 'GCTGA': 230873, 'GCTGT': 296673, 'GCTGC': 524677, 'GCTGG': 618599, 'GCCAA': 157701, 'GCCAT': 209452, 'GCCAC': 341282, 'GCCAG': 463707, 'GCCTA': 43657, 'GCCTT': 242758, 'GCCTC': 402355, 'GCCTG': 384912, 'GCCCA': 386111, 'GCCCT': 321335, 'GCCCC': 414669, 'GCCCG': 274574, 'GCCGA': 127495, 'GCCGT': 186100, 'GCCGC': 361195, 'GCCGG': 324044, 'GCGAA': 55189, 'GCGAT': 79427, 'GCGAC': 69124, 'GCGAG': 128660, 'GCGTA': 47545, 'GCGTT': 82349, 'GCGTC': 152039, 'GCGTG': 179205, 'GCGCA': 165115, 'GCGCT': 188980, 'GCGCC': 241461, 'GCGCG': 147689, 'GCGGA': 177672, 'GCGGT': 177050, 'GCGGC': 366696, 'GCGGG': 326468, 'GGAAA': 181249, 'GGAAT': 120270, 'GGAAC': 165961, 'GGAAG': 394860, 'GGATA': 53271, 'GGATT': 94405, 'GGATC': 192943, 'GGATG': 328002, 'GGACA': 239081, 'GGACT': 186100, 'GGACC': 213014, 'GGACG': 131837, 'GGAGA': 311951, 'GGAGT': 208054, 'GGAGC': 347577, 'GGAGG': 570852, 'GGTAA': 61101, 'GGTAT': 66993, 'GGTAC': 131871, 'GGTAG': 196800, 'GGTTA': 47771, 'GGTTT': 140215, 'GGTTC': 182613, 'GGTTG': 235681, 'GGTCA': 267515, 'GGTCT': 258348, 'GGTCC': 351076, 'GGTCG': 129823, 'GGTGA': 292021, 'GGTGT': 206207, 'GGTGC': 295466, 'GGTGG': 564567, 'GGCAA': 169436, 'GGCAT': 192964, 'GGCAC': 272372, 'GGCAG': 585593, 'GGCTA': 66599, 'GGCTT': 276827, 'GGCTC': 359095, 'GGCTG': 579666, 'GGCCA': 469653, 'GGCCT': 437644, 'GGCCC': 533922, 'GGCCG': 374163, 'GGCGA': 140542, 'GGCGT': 185953, 'GGCGC': 289116, 'GGCGG': 413786, 'GGGAA': 231094, 'GGGAT': 180385, 'GGGAC': 256609, 'GGGAG': 468607, 'GGGTA': 110455, 'GGGTT': 190876, 'GGGTC': 314329, 'GGGTG': 422108, 'GGGCA': 437923, 'GGGCT': 432011, 'GGGCC': 617621, 'GGGCG': 306293, 'GGGGA': 394352, 'GGGGT': 388170, 'GGGGC': 626461, 'GGGGG': 585502}, 'overrepresented_sequences': {}} {'total_reads': 2960672, 'total_bases': 171536468, 'q20_bases': 163160225, 'q30_bases': 155085906, 'total_cycles': 58, 'quality_curves': {'A': [36.1369, 36.1414, 36.1464, 36.192, 36.2114, 36.196, 36.2013, 36.2106, 36.1947, 36.1737, 36.1506, 36.1494, 36.1334, 36.1025, 36.1166, 36.0761, 36.0779, 36.0616, 36.0329, 36.021, 35.9731, 35.9709, 35.9777, 35.9461, 35.9079, 35.8579, 35.8265, 35.78, 35.7705, 35.6773, 35.6473, 35.6196, 35.5717, 35.5604, 35.5327, 35.4854, 35.4538, 35.4177, 35.3787, 35.3821, 35.3931, 35.3868, 35.2918, 35.1913, 35.1364, 35.0578, 34.9686, 34.9241, 34.8518, 34.6868, 34.5048, 34.2797, 34.1589, 34.0268, 33.9908, 33.7974, 33.6107, 33.442], 'T': [36.6431, 36.7087, 36.6636, 36.6684, 36.6644, 36.662, 36.6189, 36.6257, 36.5871, 36.5532, 36.5301, 36.5086, 36.4875, 36.4601, 36.4401, 36.4168, 36.3989, 36.3731, 36.3262, 36.2966, 36.2647, 36.2547, 36.2437, 36.1872, 36.1534, 36.0882, 36.0809, 35.9985, 35.9528, 35.9295, 35.8684, 35.8492, 35.7891, 35.7585, 35.7118, 35.6926, 35.6241, 35.5797, 35.5342, 35.48, 35.4923, 35.4954, 35.4178, 35.341, 35.2501, 35.1515, 35.0963, 35.0231, 34.9262, 34.7914, 34.6331, 34.4185, 34.2634, 34.1986, 34.1135, 33.9922, 33.7536, 33.6264], 'C': [36.6363, 36.653, 36.6664, 36.6663, 36.656, 36.6705, 36.681, 36.6882, 36.6796, 36.6428, 36.6376, 36.6317, 36.6318, 36.6301, 36.6227, 36.6157, 36.6208, 36.5926, 36.5997, 36.5576, 36.5572, 36.5545, 36.5361, 36.5484, 36.5184, 36.4874, 36.4634, 36.445, 36.4009, 36.3868, 36.3444, 36.3214, 36.313, 36.2917, 36.2759, 36.2676, 36.226, 36.1769, 36.1263, 36.1433, 36.1174, 36.1037, 36.043, 35.9732, 35.9104, 35.8303, 35.7341, 35.6951, 35.5606, 35.4676, 35.3608, 35.1794, 34.9792, 34.9035, 34.7879, 34.661, 34.4523, 34.2503], 'G': [36.038, 36.0742, 36.0818, 36.0956, 36.1045, 36.1218, 36.1241, 36.115, 36.075, 36.0719, 36.0525, 36.0376, 36.0318, 36.0266, 36.0085, 35.9854, 35.984, 35.9647, 35.9476, 35.9133, 35.8895, 35.8884, 35.8647, 35.8542, 35.8297, 35.7697, 35.7635, 35.7102, 35.6814, 35.6129, 35.5966, 35.6014, 35.5567, 35.5448, 35.5032, 35.4779, 35.4339, 35.3971, 35.3557, 35.3579, 35.3209, 35.3426, 35.2685, 35.1903, 35.0964, 35.0333, 34.9328, 34.8881, 34.7751, 34.6675, 34.481, 34.2842, 34.1035, 33.9984, 33.9537, 33.7312, 33.499, 33.4119], 'mean': [36.3598, 36.3867, 36.384, 36.393, 36.3988, 36.4041, 36.3988, 36.4066, 36.3809, 36.3553, 36.3407, 36.3293, 36.3194, 36.3066, 36.2989, 36.2749, 36.2743, 36.2538, 36.2331, 36.2027, 36.1773, 36.1723, 36.1629, 36.1477, 36.1144, 36.0652, 36.0487, 36.0001, 35.967, 35.9228, 35.8798, 35.8657, 35.8301, 35.8078, 35.779, 35.7594, 35.7133, 35.6688, 35.6273, 35.6217, 35.6075, 35.6111, 35.5323, 35.4515, 35.3727, 35.2939, 35.2115, 35.1646, 35.0562, 34.9332, 34.7822, 34.5709, 34.4085, 34.3196, 34.2418, 34.0738, 33.8622, 33.7112]}, 'content_curves': {'A': [0.212154, 0.21706, 0.214655, 0.218335, 0.225862, 0.215071, 0.218022, 0.220277, 0.217977, 0.220408, 0.224455, 0.220028, 0.218438, 0.222282, 0.214675, 0.217308, 0.221384, 0.217237, 0.21916, 0.22114, 0.218193, 0.218135, 0.221597, 0.215532, 0.220431, 0.220954, 0.215153, 0.21935, 0.222091, 0.217779, 0.221334, 0.222662, 0.217555, 0.219271, 0.222658, 0.215672, 0.216089, 0.223473, 0.219555, 0.220861, 0.225591, 0.21664, 0.219831, 0.221063, 0.219471, 0.2185, 0.224447, 0.218446, 0.220475, 0.221403, 0.215121, 0.216483, 0.220331, 0.214549, 0.218953, 0.223682, 0.215839, 0.220024], 'T': [0.205949, 0.197956, 0.197501, 0.190706, 0.193075, 0.191868, 0.189654, 0.198212, 0.195672, 0.192425, 0.199716, 0.195121, 0.192698, 0.19755, 0.195891, 0.19428, 0.195365, 0.199306, 0.193821, 0.196926, 0.192642, 0.189704, 0.19598, 0.190537, 0.193279, 0.196589, 0.194322, 0.193366, 0.197118, 0.192772, 0.191262, 0.196891, 0.194408, 0.193395, 0.195744, 0.195812, 0.191309, 0.194588, 0.193053, 0.190073, 0.192218, 0.193354, 0.192414, 0.198223, 0.1917, 0.19192, 0.194964, 0.192884, 0.190973, 0.196913, 0.194458, 0.190633, 0.195932, 0.195605, 0.191636, 0.195061, 0.194284, 0.191668], 'C': [0.297685, 0.297776, 0.296599, 0.292771, 0.293858, 0.296471, 0.294833, 0.295381, 0.297144, 0.294935, 0.291938, 0.294918, 0.295988, 0.294357, 0.297626, 0.297178, 0.295917, 0.298413, 0.296726, 0.297091, 0.295755, 0.296261, 0.296157, 0.302855, 0.297578, 0.297289, 0.30038, 0.297833, 0.295057, 0.303438, 0.298009, 0.293698, 0.299533, 0.296524, 0.295672, 0.301576, 0.301546, 0.297534, 0.301568, 0.299517, 0.297973, 0.302009, 0.298373, 0.298277, 0.300279, 0.300284, 0.297926, 0.300692, 0.299611, 0.29631, 0.302907, 0.299987, 0.298727, 0.304876, 0.299936, 0.298644, 0.303873, 0.300012], 'G': [0.284157, 0.287208, 0.291245, 0.298189, 0.287205, 0.296591, 0.297486, 0.28613, 0.289207, 0.292232, 0.283888, 0.289933, 0.292876, 0.285807, 0.291805, 0.291194, 0.287333, 0.285024, 0.290293, 0.284804, 0.293405, 0.295872, 0.286265, 0.291076, 0.288712, 0.285168, 0.290137, 0.289451, 0.285734, 0.286011, 0.289311, 0.28675, 0.288459, 0.290713, 0.285926, 0.286931, 0.29105, 0.284392, 0.285817, 0.289546, 0.284218, 0.287982, 0.289347, 0.282362, 0.288359, 0.28909, 0.282662, 0.287974, 0.288941, 0.285374, 0.287514, 0.292696, 0.285005, 0.28497, 0.289446, 0.282587, 0.286005, 0.288296], 'N': [5.50551e-05, 0, 0, 0, 0, 0, 4.72866e-06, 0, 0, 0, 1.68881e-06, 3.37761e-07, 0, 2.70209e-06, 2.70209e-06, 4.08691e-05, 0, 1.95901e-05, 1.35104e-06, 3.98558e-05, 5.40418e-06, 2.80342e-05, 0, 0, 0, 0, 9.11961e-06, 6.7553e-07, 0, 0, 8.37664e-05, 0, 4.59411e-05, 9.72913e-05, 0, 9.45984e-06, 5.74378e-06, 1.38536e-05, 6.75835e-06, 2.02765e-06, 0, 1.58859e-05, 3.44789e-05, 7.36976e-05, 0.000192047, 0.000205263, 0, 3.38293e-06, 0, 0, 0, 0.000201624, 5.42569e-06, 3.39763e-07, 2.82183e-05, 2.65385e-05, 0, 0], 'GC': [0.581843, 0.584984, 0.587844, 0.590959, 0.581063, 0.593062, 0.592319, 0.581511, 0.586351, 0.587167, 0.575826, 0.584851, 0.588864, 0.580165, 0.589431, 0.588371, 0.583251, 0.583437, 0.587018, 0.581895, 0.589159, 0.592133, 0.582423, 0.593931, 0.58629, 0.582457, 0.590517, 0.587283, 0.580791, 0.589449, 0.587321, 0.580448, 0.587992, 0.587237, 0.581598, 0.588506, 0.592596, 0.581926, 0.587385, 0.589063, 0.582191, 0.589991, 0.58772, 0.58064, 0.588637, 0.589374, 0.580589, 0.588666, 0.588552, 0.581684, 0.590421, 0.592682, 0.583732, 0.589846, 0.589383, 0.581231, 0.589877, 0.588308]}, 'kmer_count': {'AAAAA': 266586, 'AAAAT': 112869, 'AAAAC': 138340, 'AAAAG': 159352, 'AAATA': 86335, 'AAATT': 89343, 'AAATC': 78107, 'AAATG': 105043, 'AAACA': 151664, 'AAACT': 108135, 'AAACC': 126832, 'AAACG': 61217, 'AAAGA': 216935, 'AAAGT': 117329, 'AAAGC': 147349, 'AAAGG': 142303, 'AATAA': 56746, 'AATAT': 53821, 'AATAC': 57244, 'AATAG': 37777, 'AATTA': 49900, 'AATTT': 90906, 'AATTC': 69341, 'AATTG': 56846, 'AATCA': 57412, 'AATCT': 70273, 'AATCC': 88215, 'AATCG': 23462, 'AATGA': 93834, 'AATGT': 97557, 'AATGC': 81314, 'AATGG': 115877, 'AACAA': 182799, 'AACAT': 150789, 'AACAC': 106984, 'AACAG': 157402, 'AACTA': 54063, 'AACTT': 115830, 'AACTC': 100361, 'AACTG': 137991, 'AACCA': 155794, 'AACCT': 140138, 'AACCC': 163824, 'AACCG': 69315, 'AACGA': 65145, 'AACGT': 69370, 'AACGC': 72819, 'AACGG': 84778, 'AAGAA': 404420, 'AAGAT': 227551, 'AAGAC': 205625, 'AAGAG': 259969, 'AAGTA': 96340, 'AAGTT': 138599, 'AAGTC': 135649, 'AAGTG': 136634, 'AAGCA': 189022, 'AAGCT': 218267, 'AAGCC': 240020, 'AAGCG': 122107, 'AAGGA': 302902, 'AAGGT': 167010, 'AAGGC': 228771, 'AAGGG': 188477, 'ATAAA': 72012, 'ATAAT': 32886, 'ATAAC': 38355, 'ATAAG': 37740, 'ATATA': 35959, 'ATATT': 52104, 'ATATC': 46341, 'ATATG': 43709, 'ATACA': 58823, 'ATACT': 44593, 'ATACC': 48789, 'ATACG': 22074, 'ATAGA': 39243, 'ATAGT': 30460, 'ATAGC': 30241, 'ATAGG': 34870, 'ATTAA': 56272, 'ATTAT': 42665, 'ATTAC': 57770, 'ATTAG': 23157, 'ATTTA': 58956, 'ATTTT': 130910, 'ATTTC': 102033, 'ATTTG': 83808, 'ATTCA': 85950, 'ATTCT': 86480, 'ATTCC': 90219, 'ATTCG': 42218, 'ATTGA': 108829, 'ATTGT': 78112, 'ATTGC': 71819, 'ATTGG': 61580, 'ATCAA': 163679, 'ATCAT': 139756, 'ATCAC': 107914, 'ATCAG': 96449, 'ATCTA': 62896, 'ATCTT': 129343, 'ATCTC': 126459, 'ATCTG': 143667, 'ATCCA': 155144, 'ATCCT': 173263, 'ATCCC': 151452, 'ATCCG': 116533, 'ATCGA': 92654, 'ATCGT': 57992, 'ATCGC': 65338, 'ATCGG': 59423, 'ATGAA': 174588, 'ATGAT': 142947, 'ATGAC': 129530, 'ATGAG': 197490, 'ATGTA': 58991, 'ATGTT': 86163, 'ATGTC': 119001, 'ATGTG': 159782, 'ATGCA': 118948, 'ATGCT': 121034, 'ATGCC': 176617, 'ATGCG': 59023, 'ATGGA': 187989, 'ATGGT': 129350, 'ATGGC': 208030, 'ATGGG': 168139, 'ACAAA': 157140, 'ACAAT': 62421, 'ACAAC': 155542, 'ACAAG': 278986, 'ACATA': 36831, 'ACATT': 89285, 'ACATC': 205788, 'ACATG': 162734, 'ACACA': 143418, 'ACACT': 88905, 'ACACC': 165584, 'ACACG': 85588, 'ACAGA': 184392, 'ACAGT': 115252, 'ACAGC': 253248, 'ACAGG': 177481, 'ACTAA': 41378, 'ACTAT': 53099, 'ACTAC': 115481, 'ACTAG': 20970, 'ACTTA': 39510, 'ACTTT': 114550, 'ACTTC': 185365, 'ACTTG': 86355, 'ACTCA': 103104, 'ACTCT': 101637, 'ACTCC': 173492, 'ACTCG': 59330, 'ACTGA': 131256, 'ACTGT': 117469, 'ACTGC': 173321, 'ACTGG': 193262, 'ACCAA': 201508, 'ACCAT': 184855, 'ACCAC': 184012, 'ACCAG': 305665, 'ACCTA': 81467, 'ACCTT': 127225, 'ACCTC': 215337, 'ACCTG': 339041, 'ACCCA': 228673, 'ACCCT': 206611, 'ACCCC': 316826, 'ACCCG': 121031, 'ACCGA': 78422, 'ACCGT': 74171, 'ACCGC': 173588, 'ACCGG': 130444, 'ACGAA': 38088, 'ACGAT': 48622, 'ACGAC': 101056, 'ACGAG': 178342, 'ACGTA': 21775, 'ACGTT': 28465, 'ACGTC': 77097, 'ACGTG': 140394, 'ACGCA': 87757, 'ACGCT': 126821, 'ACGCC': 171956, 'ACGCG': 63552, 'ACGGA': 125917, 'ACGGT': 55298, 'ACGGC': 167693, 'ACGGG': 122342, 'AGAAA': 250846, 'AGAAT': 107924, 'AGAAC': 208225, 'AGAAG': 488442, 'AGATA': 54996, 'AGATT': 102350, 'AGATC': 213660, 'AGATG': 254219, 'AGACA': 180573, 'AGACT': 126945, 'AGACC': 220850, 'AGACG': 143377, 'AGAGA': 239647, 'AGAGT': 128020, 'AGAGC': 243253, 'AGAGG': 282772, 'AGTAA': 38032, 'AGTAT': 71319, 'AGTAC': 126993, 'AGTAG': 48878, 'AGTTA': 49803, 'AGTTT': 113926, 'AGTTC': 181619, 'AGTTG': 80818, 'AGTCA': 107043, 'AGTCT': 92238, 'AGTCC': 171143, 'AGTCG': 63146, 'AGTGA': 157713, 'AGTGT': 100488, 'AGTGC': 159997, 'AGTGG': 213802, 'AGCAA': 158295, 'AGCAT': 106809, 'AGCAC': 205175, 'AGCAG': 403630, 'AGCTA': 73862, 'AGCTT': 121849, 'AGCTC': 243745, 'AGCTG': 453987, 'AGCCA': 305774, 'AGCCT': 286861, 'AGCCC': 377827, 'AGCCG': 208706, 'AGCGA': 102317, 'AGCGT': 83599, 'AGCGC': 179723, 'AGCGG': 239321, 'AGGAA': 233681, 'AGGAT': 155805, 'AGGAC': 293591, 'AGGAG': 538166, 'AGGTA': 59833, 'AGGTT': 93282, 'AGGTC': 160026, 'AGGTG': 291645, 'AGGCA': 200268, 'AGGCT': 212365, 'AGGCC': 398942, 'AGGCG': 173500, 'AGGGA': 168702, 'AGGGT': 136208, 'AGGGC': 282242, 'AGGGG': 204873, 'TAAAA': 88275, 'TAAAT': 46395, 'TAAAC': 44573, 'TAAAG': 88607, 'TAATA': 21108, 'TAATT': 46956, 'TAATC': 30563, 'TAATG': 52258, 'TAACA': 54406, 'TAACT': 48332, 'TAACC': 40957, 'TAACG': 14410, 'TAAGA': 64868, 'TAAGT': 32185, 'TAAGC': 42125, 'TAAGG': 45064, 'TATAA': 34666, 'TATAT': 43046, 'TATAC': 27964, 'TATAG': 23034, 'TATTA': 35907, 'TATTT': 106659, 'TATTC': 47294, 'TATTG': 50326, 'TATCA': 51459, 'TATCT': 69513, 'TATCC': 53936, 'TATCG': 17685, 'TATGA': 85414, 'TATGT': 68414, 'TATGC': 66682, 'TATGG': 79883, 'TACAA': 134963, 'TACAT': 78674, 'TACAC': 74621, 'TACAG': 101315, 'TACTA': 45896, 'TACTT': 69519, 'TACTC': 52146, 'TACTG': 91205, 'TACCA': 108253, 'TACCT': 116771, 'TACCC': 95646, 'TACCG': 79327, 'TACGA': 82468, 'TACGT': 30823, 'TACGC': 53820, 'TACGG': 63522, 'TAGAA': 49209, 'TAGAT': 29597, 'TAGAC': 37957, 'TAGAG': 67632, 'TAGTA': 21632, 'TAGTT': 28062, 'TAGTC': 31506, 'TAGTG': 47643, 'TAGCA': 42413, 'TAGCT': 50456, 'TAGCC': 59506, 'TAGCG': 19695, 'TAGGA': 53105, 'TAGGT': 25495, 'TAGGC': 36664, 'TAGGG': 40103, 'TTAAA': 83684, 'TTAAT': 47975, 'TTAAC': 46339, 'TTAAG': 54918, 'TTATA': 36350, 'TTATT': 79800, 'TTATC': 42318, 'TTATG': 60478, 'TTACA': 62868, 'TTACT': 58396, 'TTACC': 51237, 'TTACG': 16463, 'TTAGA': 36646, 'TTAGT': 30906, 'TTAGC': 45637, 'TTAGG': 35862, 'TTTAA': 86636, 'TTTAT': 94866, 'TTTAC': 47652, 'TTTAG': 43824, 'TTTTA': 117024, 'TTTTT': 254129, 'TTTTC': 120374, 'TTTTG': 143697, 'TTTCA': 112272, 'TTTCT': 157180, 'TTTCC': 147200, 'TTTCG': 45660, 'TTTGA': 139879, 'TTTGT': 137888, 'TTTGC': 122917, 'TTTGG': 159877, 'TTCAA': 160392, 'TTCAT': 119416, 'TTCAC': 138503, 'TTCAG': 158540, 'TTCTA': 84369, 'TTCTT': 149061, 'TTCTC': 193996, 'TTCTG': 191030, 'TTCCA': 184864, 'TTCCT': 250549, 'TTCCC': 198152, 'TTCCG': 98285, 'TTCGA': 91727, 'TTCGT': 61866, 'TTCGC': 57593, 'TTCGG': 83536, 'TTGAA': 110813, 'TTGAT': 72825, 'TTGAC': 104075, 'TTGAG': 124842, 'TTGTA': 59645, 'TTGTT': 88974, 'TTGTC': 92743, 'TTGTG': 144555, 'TTGCA': 95472, 'TTGCT': 108469, 'TTGCC': 142234, 'TTGCG': 43250, 'TTGGA': 125608, 'TTGGT': 78580, 'TTGGC': 130988, 'TTGGG': 143891, 'TCAAA': 124743, 'TCAAT': 60675, 'TCAAC': 171941, 'TCAAG': 268993, 'TCATA': 39038, 'TCATT': 84851, 'TCATC': 216894, 'TCATG': 178575, 'TCACA': 116244, 'TCACT': 126457, 'TCACC': 247592, 'TCACG': 79526, 'TCAGA': 160742, 'TCAGT': 109951, 'TCAGC': 209769, 'TCAGG': 170056, 'TCTAA': 34042, 'TCTAT': 57094, 'TCTAC': 142816, 'TCTAG': 54776, 'TCTTA': 49394, 'TCTTT': 149254, 'TCTTC': 240114, 'TCTTG': 107882, 'TCTCA': 149293, 'TCTCT': 177022, 'TCTCC': 266943, 'TCTCG': 82523, 'TCTGA': 139638, 'TCTGT': 163885, 'TCTGC': 227347, 'TCTGG': 265014, 'TCCAA': 174698, 'TCCAT': 121019, 'TCCAC': 225843, 'TCCAG': 320400, 'TCCTA': 87683, 'TCCTT': 152740, 'TCCTC': 289237, 'TCCTG': 410679, 'TCCCA': 245356, 'TCCCT': 223155, 'TCCCC': 326362, 'TCCCG': 165652, 'TCCGA': 93721, 'TCCGT': 73820, 'TCCGC': 173050, 'TCCGG': 167772, 'TCGAA': 44549, 'TCGAT': 33555, 'TCGAC': 86864, 'TCGAG': 130099, 'TCGTA': 22852, 'TCGTT': 23577, 'TCGTC': 76707, 'TCGTG': 121783, 'TCGCA': 49632, 'TCGCT': 75116, 'TCGCC': 124203, 'TCGCG': 58319, 'TCGGA': 92494, 'TCGGT': 57741, 'TCGGC': 140819, 'TCGGG': 129953, 'TGAAA': 136041, 'TGAAT': 82994, 'TGAAC': 151568, 'TGAAG': 344311, 'TGATA': 46832, 'TGATT': 73408, 'TGATC': 129029, 'TGATG': 205013, 'TGACA': 152832, 'TGACT': 108691, 'TGACC': 242986, 'TGACG': 94345, 'TGAGA': 157453, 'TGAGT': 113024, 'TGAGC': 233305, 'TGAGG': 276478, 'TGTAA': 67696, 'TGTAT': 64428, 'TGTAC': 91376, 'TGTAG': 52614, 'TGTTA': 53304, 'TGTTT': 135598, 'TGTTC': 132072, 'TGTTG': 107180, 'TGTCA': 127977, 'TGTCT': 140304, 'TGTCC': 211865, 'TGTCG': 64836, 'TGTGA': 177290, 'TGTGT': 164363, 'TGTGC': 207262, 'TGTGG': 280154, 'TGCAA': 122291, 'TGCAT': 88143, 'TGCAC': 154617, 'TGCAG': 361744, 'TGCTA': 62786, 'TGCTT': 124040, 'TGCTC': 184637, 'TGCTG': 454598, 'TGCCA': 254731, 'TGCCT': 268782, 'TGCCC': 363623, 'TGCCG': 169245, 'TGCGA': 70411, 'TGCGT': 72413, 'TGCGC': 160465, 'TGCGG': 167962, 'TGGAA': 185515, 'TGGAT': 151530, 'TGGAC': 302179, 'TGGAG': 489194, 'TGGTA': 65561, 'TGGTT': 95408, 'TGGTC': 182222, 'TGGTG': 341244, 'TGGCA': 216581, 'TGGCT': 258661, 'TGGCC': 443207, 'TGGCG': 184566, 'TGGGA': 241818, 'TGGGT': 161760, 'TGGGC': 357057, 'TGGGG': 317494, 'CAAAA': 154345, 'CAAAT': 97874, 'CAAAC': 120854, 'CAAAG': 200026, 'CAATA': 44502, 'CAATT': 51885, 'CAATC': 59163, 'CAATG': 112663, 'CAACA': 228610, 'CAACT': 122301, 'CAACC': 197654, 'CAACG': 117201, 'CAAGA': 383313, 'CAAGT': 188982, 'CAAGC': 261442, 'CAAGG': 372450, 'CATAA': 39968, 'CATAT': 35592, 'CATAC': 46267, 'CATAG': 39078, 'CATTA': 50271, 'CATTT': 92805, 'CATTC': 94076, 'CATTG': 133158, 'CATCA': 261589, 'CATCT': 214441, 'CATCC': 271187, 'CATCG': 160532, 'CATGA': 187813, 'CATGT': 137184, 'CATGC': 169118, 'CATGG': 274789, 'CACAA': 137114, 'CACAT': 103237, 'CACAC': 156807, 'CACAG': 252770, 'CACTA': 67546, 'CACTT': 117282, 'CACTC': 124554, 'CACTG': 222177, 'CACCA': 328867, 'CACCT': 278895, 'CACCC': 331333, 'CACCG': 177156, 'CACGA': 85761, 'CACGT': 79255, 'CACGC': 171596, 'CACGG': 159640, 'CAGAA': 255646, 'CAGAT': 180497, 'CAGAC': 213153, 'CAGAG': 319203, 'CAGTA': 83141, 'CAGTT': 133262, 'CAGTC': 126351, 'CAGTG': 267711, 'CAGCA': 385970, 'CAGCT': 342351, 'CAGCC': 531454, 'CAGCG': 246410, 'CAGGA': 320549, 'CAGGT': 204999, 'CAGGC': 342718, 'CAGGG': 279192, 'CTAAA': 59790, 'CTAAT': 32339, 'CTAAC': 31543, 'CTAAG': 58905, 'CTATA': 33970, 'CTATT': 56202, 'CTATC': 61740, 'CTATG': 116973, 'CTACA': 170095, 'CTACT': 90070, 'CTACC': 190529, 'CTACG': 141834, 'CTAGA': 51645, 'CTAGT': 33874, 'CTAGC': 48532, 'CTAGG': 47621, 'CTTAA': 50526, 'CTTAT': 48545, 'CTTAC': 41719, 'CTTAG': 48476, 'CTTTA': 53031, 'CTTTT': 147319, 'CTTTC': 148741, 'CTTTG': 192419, 'CTTCA': 251968, 'CTTCT': 241506, 'CTTCC': 351536, 'CTTCG': 134664, 'CTTGA': 101256, 'CTTGT': 94408, 'CTTGC': 115433, 'CTTGG': 149232, 'CTCAA': 184353, 'CTCAT': 149812, 'CTCAC': 190478, 'CTCAG': 247284, 'CTCTA': 79549, 'CTCTT': 158604, 'CTCTC': 206695, 'CTCTG': 283305, 'CTCCA': 323972, 'CTCCT': 320995, 'CTCCC': 374326, 'CTCCG': 191109, 'CTCGA': 61925, 'CTCGT': 58871, 'CTCGC': 108768, 'CTCGG': 181626, 'CTGAA': 235449, 'CTGAT': 139698, 'CTGAC': 192647, 'CTGAG': 289336, 'CTGTA': 97332, 'CTGTT': 136065, 'CTGTC': 201703, 'CTGTG': 310850, 'CTGCA': 332936, 'CTGCT': 396124, 'CTGCC': 499822, 'CTGCG': 237513, 'CTGGA': 488880, 'CTGGT': 262323, 'CTGGC': 437811, 'CTGGG': 482734, 'CCAAA': 164987, 'CCAAT': 80074, 'CCAAC': 216770, 'CCAAG': 389792, 'CCATA': 56779, 'CCATT': 111902, 'CCATC': 303047, 'CCATG': 279246, 'CCACA': 241350, 'CCACT': 187986, 'CCACC': 445247, 'CCACG': 201643, 'CCAGA': 349109, 'CCAGT': 207820, 'CCAGC': 549264, 'CCAGG': 463472, 'CCTAA': 57459, 'CCTAT': 85662, 'CCTAC': 184709, 'CCTAG': 62165, 'CCTTA': 60863, 'CCTTT': 150429, 'CCTTC': 321186, 'CCTTG': 159883, 'CCTCA': 303952, 'CCTCT': 261303, 'CCTCC': 469514, 'CCTCG': 172432, 'CCTGA': 291598, 'CCTGT': 223473, 'CCTGC': 543128, 'CCTGG': 675835, 'CCCAA': 227556, 'CCCAT': 176168, 'CCCAC': 337764, 'CCCAG': 553410, 'CCCTA': 112402, 'CCCTT': 198511, 'CCCTC': 337547, 'CCCTG': 521205, 'CCCCA': 456997, 'CCCCT': 365733, 'CCCCC': 568924, 'CCCCG': 321179, 'CCCGA': 182333, 'CCCGT': 116184, 'CCCGC': 290692, 'CCCGG': 332431, 'CCGAA': 73285, 'CCGAT': 51346, 'CCGAC': 154583, 'CCGAG': 263304, 'CCGTA': 33512, 'CCGTT': 46257, 'CCGTC': 132529, 'CCGTG': 180510, 'CCGCA': 201492, 'CCGCT': 195504, 'CCGCC': 420584, 'CCGCG': 202574, 'CCGGA': 199918, 'CCGGT': 74396, 'CCGGC': 302569, 'CCGGG': 336010, 'CGAAA': 37577, 'CGAAT': 37871, 'CGAAC': 46530, 'CGAAG': 81248, 'CGATA': 21400, 'CGATT': 35434, 'CGATC': 36968, 'CGATG': 90070, 'CGACA': 114290, 'CGACT': 82358, 'CGACC': 140268, 'CGACG': 109062, 'CGAGA': 179018, 'CGAGT': 107993, 'CGAGC': 186575, 'CGAGG': 282546, 'CGTAA': 22208, 'CGTAT': 16169, 'CGTAC': 35593, 'CGTAG': 23240, 'CGTTA': 11107, 'CGTTT': 37944, 'CGTTC': 44836, 'CGTTG': 45777, 'CGTCA': 112147, 'CGTCT': 108228, 'CGTCC': 131020, 'CGTCG': 52695, 'CGTGA': 96858, 'CGTGT': 90445, 'CGTGC': 168385, 'CGTGG': 250818, 'CGCAA': 123682, 'CGCAT': 97290, 'CGCAC': 107928, 'CGCAG': 162768, 'CGCTA': 67953, 'CGCTT': 92840, 'CGCTC': 135903, 'CGCTG': 268906, 'CGCCA': 244192, 'CGCCT': 231305, 'CGCCC': 279040, 'CGCCG': 236519, 'CGCGA': 82201, 'CGCGT': 63607, 'CGCGC': 155576, 'CGCGG': 178232, 'CGGAA': 143232, 'CGGAT': 69680, 'CGGAC': 136817, 'CGGAG': 267561, 'CGGTA': 27922, 'CGGTT': 43670, 'CGGTC': 74212, 'CGGTG': 123784, 'CGGCA': 203154, 'CGGCT': 193904, 'CGGCC': 369252, 'CGGCG': 257584, 'CGGGA': 213793, 'CGGGT': 97844, 'CGGGC': 264901, 'CGGGG': 262950, 'GAAAA': 170212, 'GAAAT': 102410, 'GAAAC': 144777, 'GAAAG': 176150, 'GAATA': 53889, 'GAATT': 78893, 'GAATC': 72365, 'GAATG': 118661, 'GAACA': 163827, 'GAACT': 129450, 'GAACC': 163422, 'GAACG': 99528, 'GAAGA': 435445, 'GAAGT': 167859, 'GAAGC': 319576, 'GAAGG': 327442, 'GATAA': 49676, 'GATAT': 46235, 'GATAC': 42202, 'GATAG': 34655, 'GATTA': 43768, 'GATTT': 86009, 'GATTC': 93837, 'GATTG': 80618, 'GATCA': 137659, 'GATCT': 107594, 'GATCC': 183018, 'GATCG': 73487, 'GATGA': 277193, 'GATGT': 121376, 'GATGC': 158463, 'GATGG': 222224, 'GACAA': 200910, 'GACAT': 161689, 'GACAC': 145584, 'GACAG': 219040, 'GACTA': 63333, 'GACTT': 123463, 'GACTC': 160990, 'GACTG': 162394, 'GACCA': 284744, 'GACCT': 227916, 'GACCC': 286014, 'GACCG': 131821, 'GACGA': 133135, 'GACGT': 89013, 'GACGC': 152059, 'GACGG': 163208, 'GAGAA': 348408, 'GAGAT': 187986, 'GAGAC': 214526, 'GAGAG': 245468, 'GAGTA': 85031, 'GAGTT': 125873, 'GAGTC': 139948, 'GAGTG': 179847, 'GAGCA': 255646, 'GAGCT': 284022, 'GAGCC': 349600, 'GAGCG': 217370, 'GAGGA': 544859, 'GAGGT': 206264, 'GAGGC': 377043, 'GAGGG': 283540, 'GTAAA': 52853, 'GTAAT': 37692, 'GTAAC': 41927, 'GTAAG': 33172, 'GTATA': 22461, 'GTATT': 53107, 'GTATC': 42123, 'GTATG': 79021, 'GTACA': 97207, 'GTACT': 65136, 'GTACC': 110041, 'GTACG': 49815, 'GTAGA': 56429, 'GTAGT': 32869, 'GTAGC': 47973, 'GTAGG': 36737, 'GTTAA': 39334, 'GTTAT': 32999, 'GTTAC': 41552, 'GTTAG': 32492, 'GTTTA': 43255, 'GTTTT': 101552, 'GTTTC': 91721, 'GTTTG': 141815, 'GTTCA': 125931, 'GTTCT': 132699, 'GTTCC': 141834, 'GTTCG': 71095, 'GTTGA': 63004, 'GTTGT': 75102, 'GTTGC': 77992, 'GTTGG': 107186, 'GTCAA': 116361, 'GTCAT': 108946, 'GTCAC': 131840, 'GTCAG': 147523, 'GTCTA': 61451, 'GTCTT': 108436, 'GTCTC': 147494, 'GTCTG': 175589, 'GTCCA': 176746, 'GTCCT': 195680, 'GTCCC': 235583, 'GTCCG': 99355, 'GTCGA': 47728, 'GTCGT': 66118, 'GTCGC': 75099, 'GTCGG': 93103, 'GTGAA': 193783, 'GTGAT': 98589, 'GTGAC': 172015, 'GTGAG': 165074, 'GTGTA': 60387, 'GTGTT': 115796, 'GTGTC': 129731, 'GTGTG': 211936, 'GTGCA': 178412, 'GTGCT': 200424, 'GTGCC': 239826, 'GTGCG': 130787, 'GTGGA': 323785, 'GTGGT': 214238, 'GTGGC': 324057, 'GTGGG': 282142, 'GCAAA': 127050, 'GCAAT': 65290, 'GCAAC': 123311, 'GCAAG': 268846, 'GCATA': 27741, 'GCATT': 83626, 'GCATC': 183677, 'GCATG': 147651, 'GCACA': 149754, 'GCACT': 129214, 'GCACC': 259893, 'GCACG': 129819, 'GCAGA': 274936, 'GCAGT': 178066, 'GCAGC': 493752, 'GCAGG': 337219, 'GCTAA': 49638, 'GCTAT': 73640, 'GCTAC': 148631, 'GCTAG': 43600, 'GCTTA': 39532, 'GCTTT': 127405, 'GCTTC': 233812, 'GCTTG': 104593, 'GCTCA': 215468, 'GCTCT': 188169, 'GCTCC': 302876, 'GCTCG': 95800, 'GCTGA': 294720, 'GCTGT': 238692, 'GCTGC': 524391, 'GCTGG': 537041, 'GCCAA': 248625, 'GCCAT': 267653, 'GCCAC': 328963, 'GCCAG': 390299, 'GCCTA': 108327, 'GCCTT': 213244, 'GCCTC': 364072, 'GCCTG': 461211, 'GCCCA': 365242, 'GCCCT': 374127, 'GCCCC': 508833, 'GCCCG': 313285, 'GCCGA': 188232, 'GCCGT': 128924, 'GCCGC': 381653, 'GCCGG': 280710, 'GCGAA': 47337, 'GCGAT': 50117, 'GCGAC': 103332, 'GCGAG': 184031, 'GCGTA': 19005, 'GCGTT': 41056, 'GCGTC': 117871, 'GCGTG': 165520, 'GCGCA': 152323, 'GCGCT': 168020, 'GCGCC': 275422, 'GCGCG': 153520, 'GCGGA': 195549, 'GCGGT': 81989, 'GCGGC': 411496, 'GCGGG': 251586, 'GGAAA': 168679, 'GGAAT': 95186, 'GGAAC': 150367, 'GGAAG': 339648, 'GGATA': 49748, 'GGATT': 93407, 'GGATC': 118310, 'GGATG': 229811, 'GGACA': 280113, 'GGACT': 192384, 'GGACC': 329204, 'GGACG': 191286, 'GGAGA': 420050, 'GGAGT': 183097, 'GGAGC': 441365, 'GGAGG': 568960, 'GGTAA': 38099, 'GGTAT': 45179, 'GGTAC': 68723, 'GGTAG': 49346, 'GGTTA': 32492, 'GGTTT': 91915, 'GGTTC': 112449, 'GGTTG': 88608, 'GGTCA': 155945, 'GGTCT': 151334, 'GGTCC': 193840, 'GGTCG': 100827, 'GGTGA': 196597, 'GGTGT': 163039, 'GGTGC': 215093, 'GGTGG': 400704, 'GGCAA': 180986, 'GGCAT': 150415, 'GGCAC': 199937, 'GGCAG': 356730, 'GGCTA': 110790, 'GGCTT': 167099, 'GGCTC': 240253, 'GGCTG': 420694, 'GGCCA': 432191, 'GGCCT': 363209, 'GGCCC': 543587, 'GGCCG': 365255, 'GGCGA': 128910, 'GGCGT': 124260, 'GGCGC': 253066, 'GGCGG': 351621, 'GGGAA': 191911, 'GGGAT': 114681, 'GGGAC': 261033, 'GGGAG': 316033, 'GGGTA': 48570, 'GGGTT': 93006, 'GGGTC': 184571, 'GGGTG': 217835, 'GGGCA': 269640, 'GGGCT': 274193, 'GGGCC': 493382, 'GGGCG': 238457, 'GGGGA': 260759, 'GGGGT': 148453, 'GGGGC': 374396, 'GGGGG': 262470}, 'overrepresented_sequences': {}} {'passed_filter_reads': 5921344, 'low_quality_reads': 122934, 'too_many_N_reads': 1740, 'too_short_reads': 92, 'too_long_reads': 0} fastp -i /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045016_1.fastq.gz -I /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045016_2.fastq.gz -o /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045016.trimmed_1.fastq.gz -O /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045016.trimmed_2.fastq.gz -l 25 --adapter_fasta /home/ubuntu/workspace/rnaseq/team_exercise/references/illumina_multiplex.fa --trim_tail1 5 --trim_tail2 5 --json /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045016.fastp.json --html /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045016.fastp.html SRR10045017_1 {'adapter_trimmed_reads': 40610, 'adapter_trimmed_bases': 251469, 'read1_adapter_sequence': 'unspecified', 'read2_adapter_sequence': 'unspecified', 'read1_adapter_counts': {'A': 622, 'C': 700, 'G': 827, 'T': 602, 'AG': 217, 'GG': 218, 'others': 17119}, 'read2_adapter_counts': {'A': 703, 'C': 722, 'G': 739, 'T': 588, 'AG': 210, 'CC': 211, 'others': 17132}} {'rate': 0.131891} {'peak': 85, 'unknown': 938792, 'histogram': [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 40, 52, 37, 47, 47, 49, 58, 64, 76, 73, 79, 97, 116, 120, 168, 188, 216, 245, 304, 348, 407, 451, 530, 634, 705, 766, 912, 1069, 1143, 1318, 1448, 1640, 1766, 1983, 2178, 2379, 2513, 2720, 2944, 3216, 3396, 3663, 3836, 4049, 4241, 4545, 4633, 4931, 5083, 5352, 5439, 5825, 5773, 5957, 6029, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 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45730, 'ACTTT': 131693, 'ACTTC': 210392, 'ACTTG': 99669, 'ACTCA': 118430, 'ACTCT': 116797, 'ACTCC': 203657, 'ACTCG': 67980, 'ACTGA': 148429, 'ACTGT': 134186, 'ACTGC': 201717, 'ACTGG': 222385, 'ACCAA': 229663, 'ACCAT': 211979, 'ACCAC': 217395, 'ACCAG': 350951, 'ACCTA': 94553, 'ACCTT': 149113, 'ACCTC': 248929, 'ACCTG': 387809, 'ACCCA': 266141, 'ACCCT': 236836, 'ACCCC': 383740, 'ACCCG': 141700, 'ACCGA': 90816, 'ACCGT': 85711, 'ACCGC': 199763, 'ACCGG': 152763, 'ACGAA': 44659, 'ACGAT': 54614, 'ACGAC': 119099, 'ACGAG': 206993, 'ACGTA': 25745, 'ACGTT': 32683, 'ACGTC': 89742, 'ACGTG': 160999, 'ACGCA': 101105, 'ACGCT': 144032, 'ACGCC': 200424, 'ACGCG': 73775, 'ACGGA': 139375, 'ACGGT': 64275, 'ACGGC': 194168, 'ACGGG': 143159, 'AGAAA': 287228, 'AGAAT': 123568, 'AGAAC': 240461, 'AGAAG': 553847, 'AGATA': 62584, 'AGATT': 118068, 'AGATC': 249610, 'AGATG': 287191, 'AGACA': 209168, 'AGACT': 144739, 'AGACC': 255333, 'AGACG': 163183, 'AGAGA': 274917, 'AGAGT': 144429, 'AGAGC': 279135, 'AGAGG': 327194, 'AGTAA': 44440, 'AGTAT': 81467, 'AGTAC': 146430, 'AGTAG': 56845, 'AGTTA': 56932, 'AGTTT': 133358, 'AGTTC': 206940, 'AGTTG': 92642, 'AGTCA': 122089, 'AGTCT': 105499, 'AGTCC': 196112, 'AGTCG': 73205, 'AGTGA': 181851, 'AGTGT': 114139, 'AGTGC': 185508, 'AGTGG': 243938, 'AGCAA': 181227, 'AGCAT': 122430, 'AGCAC': 238534, 'AGCAG': 464200, 'AGCTA': 85715, 'AGCTT': 139770, 'AGCTC': 279281, 'AGCTG': 521689, 'AGCCA': 349921, 'AGCCT': 326276, 'AGCCC': 447320, 'AGCCG': 240209, 'AGCGA': 120287, 'AGCGT': 94137, 'AGCGC': 210405, 'AGCGG': 277728, 'AGGAA': 267807, 'AGGAT': 178992, 'AGGAC': 336448, 'AGGAG': 620247, 'AGGTA': 68452, 'AGGTT': 108143, 'AGGTC': 186186, 'AGGTG': 335535, 'AGGCA': 229617, 'AGGCT': 242454, 'AGGCC': 461613, 'AGGCG': 202499, 'AGGGA': 194902, 'AGGGT': 158791, 'AGGGC': 326065, 'AGGGG': 240800, 'TAAAA': 103545, 'TAAAT': 54926, 'TAAAC': 52352, 'TAAAG': 100540, 'TAATA': 24737, 'TAATT': 55457, 'TAATC': 36033, 'TAATG': 60435, 'TAACA': 61964, 'TAACT': 54573, 'TAACC': 48795, 'TAACG': 17184, 'TAAGA': 73653, 'TAAGT': 37024, 'TAAGC': 49426, 'TAAGG': 51905, 'TATAA': 40319, 'TATAT': 51107, 'TATAC': 33290, 'TATAG': 26364, 'TATTA': 42453, 'TATTT': 124662, 'TATTC': 54298, 'TATTG': 58541, 'TATCA': 58322, 'TATCT': 79031, 'TATCC': 62998, 'TATCG': 20863, 'TATGA': 97414, 'TATGT': 79288, 'TATGC': 76527, 'TATGG': 93223, 'TACAA': 153839, 'TACAT': 90731, 'TACAC': 86420, 'TACAG': 116508, 'TACTA': 52673, 'TACTT': 80136, 'TACTC': 60654, 'TACTG': 105116, 'TACCA': 124908, 'TACCT': 133363, 'TACCC': 111483, 'TACCG': 89974, 'TACGA': 93889, 'TACGT': 35893, 'TACGC': 60928, 'TACGG': 72439, 'TAGAA': 57343, 'TAGAT': 33856, 'TAGAC': 44046, 'TAGAG': 77413, 'TAGTA': 25283, 'TAGTT': 33205, 'TAGTC': 36503, 'TAGTG': 54017, 'TAGCA': 49315, 'TAGCT': 57141, 'TAGCC': 68800, 'TAGCG': 23028, 'TAGGA': 60946, 'TAGGT': 30294, 'TAGGC': 43123, 'TAGGG': 47456, 'TTAAA': 96701, 'TTAAT': 56029, 'TTAAC': 53512, 'TTAAG': 63383, 'TTATA': 43543, 'TTATT': 93208, 'TTATC': 49473, 'TTATG': 69383, 'TTACA': 73834, 'TTACT': 67178, 'TTACC': 59321, 'TTACG': 19136, 'TTAGA': 42441, 'TTAGT': 36509, 'TTAGC': 52087, 'TTAGG': 42182, 'TTTAA': 99718, 'TTTAT': 110986, 'TTTAC': 55786, 'TTTAG': 51170, 'TTTTA': 133703, 'TTTTT': 297599, 'TTTTC': 140313, 'TTTTG': 164006, 'TTTCA': 127717, 'TTTCT': 181540, 'TTTCC': 174625, 'TTTCG': 50948, 'TTTGA': 161796, 'TTTGT': 158213, 'TTTGC': 141502, 'TTTGG': 181793, 'TTCAA': 181666, 'TTCAT': 137403, 'TTCAC': 159037, 'TTCAG': 180548, 'TTCTA': 97385, 'TTCTT': 169715, 'TTCTC': 223853, 'TTCTG': 217673, 'TTCCA': 211059, 'TTCCT': 287916, 'TTCCC': 233086, 'TTCCG': 113601, 'TTCGA': 104440, 'TTCGT': 69250, 'TTCGC': 66309, 'TTCGG': 94924, 'TTGAA': 128087, 'TTGAT': 84352, 'TTGAC': 117571, 'TTGAG': 144099, 'TTGTA': 68633, 'TTGTT': 103040, 'TTGTC': 106717, 'TTGTG': 165207, 'TTGCA': 109823, 'TTGCT': 123510, 'TTGCC': 164617, 'TTGCG': 49426, 'TTGGA': 141028, 'TTGGT': 90854, 'TTGGC': 152656, 'TTGGG': 166736, 'TCAAA': 142080, 'TCAAT': 68644, 'TCAAC': 196552, 'TCAAG': 306510, 'TCATA': 45680, 'TCATT': 98420, 'TCATC': 251152, 'TCATG': 201101, 'TCACA': 133861, 'TCACT': 145981, 'TCACC': 286318, 'TCACG': 94227, 'TCAGA': 182391, 'TCAGT': 125829, 'TCAGC': 242337, 'TCAGG': 197165, 'TCTAA': 39904, 'TCTAT': 65938, 'TCTAC': 164428, 'TCTAG': 62511, 'TCTTA': 57755, 'TCTTT': 170075, 'TCTTC': 271231, 'TCTTG': 123616, 'TCTCA': 172315, 'TCTCT': 200721, 'TCTCC': 310076, 'TCTCG': 95160, 'TCTGA': 158681, 'TCTGT': 188530, 'TCTGC': 262085, 'TCTGG': 303691, 'TCCAA': 199856, 'TCCAT': 141628, 'TCCAC': 263659, 'TCCAG': 370164, 'TCCTA': 100029, 'TCCTT': 175940, 'TCCTC': 337009, 'TCCTG': 473845, 'TCCCA': 285249, 'TCCCT': 261672, 'TCCCC': 391514, 'TCCCG': 193449, 'TCCGA': 107545, 'TCCGT': 86712, 'TCCGC': 199032, 'TCCGG': 190342, 'TCGAA': 50612, 'TCGAT': 38361, 'TCGAC': 101543, 'TCGAG': 149203, 'TCGTA': 26342, 'TCGTT': 27421, 'TCGTC': 86813, 'TCGTG': 139711, 'TCGCA': 57977, 'TCGCT': 87015, 'TCGCC': 145300, 'TCGCG': 67897, 'TCGGA': 105137, 'TCGGT': 65960, 'TCGGC': 161367, 'TCGGG': 150386, 'TGAAA': 156079, 'TGAAT': 95932, 'TGAAC': 175240, 'TGAAG': 391853, 'TGATA': 52981, 'TGATT': 84795, 'TGATC': 146670, 'TGATG': 235798, 'TGACA': 174618, 'TGACT': 124811, 'TGACC': 278465, 'TGACG': 107486, 'TGAGA': 182783, 'TGAGT': 129344, 'TGAGC': 266578, 'TGAGG': 316533, 'TGTAA': 78232, 'TGTAT': 75467, 'TGTAC': 104298, 'TGTAG': 61109, 'TGTTA': 61471, 'TGTTT': 155046, 'TGTTC': 151999, 'TGTTG': 124595, 'TGTCA': 147267, 'TGTCT': 161501, 'TGTCC': 245263, 'TGTCG': 74949, 'TGTGA': 202870, 'TGTGT': 188746, 'TGTGC': 237077, 'TGTGG': 321003, 'TGCAA': 139391, 'TGCAT': 102077, 'TGCAC': 179671, 'TGCAG': 416705, 'TGCTA': 72440, 'TGCTT': 139140, 'TGCTC': 215240, 'TGCTG': 522898, 'TGCCA': 289352, 'TGCCT': 313582, 'TGCCC': 427959, 'TGCCG': 196707, 'TGCGA': 80983, 'TGCGT': 82923, 'TGCGC': 185586, 'TGCGG': 193650, 'TGGAA': 211419, 'TGGAT': 173639, 'TGGAC': 343965, 'TGGAG': 560131, 'TGGTA': 73831, 'TGGTT': 108476, 'TGGTC': 208653, 'TGGTG': 394988, 'TGGCA': 249512, 'TGGCT': 297732, 'TGGCC': 508565, 'TGGCG': 213950, 'TGGGA': 279208, 'TGGGT': 185028, 'TGGGC': 409207, 'TGGGG': 368958, 'CAAAA': 180510, 'CAAAT': 112332, 'CAAAC': 138876, 'CAAAG': 230946, 'CAATA': 51569, 'CAATT': 59506, 'CAATC': 68051, 'CAATG': 130077, 'CAACA': 262658, 'CAACT': 140245, 'CAACC': 227131, 'CAACG': 135768, 'CAAGA': 438130, 'CAAGT': 214825, 'CAAGC': 302357, 'CAAGG': 423128, 'CATAA': 46959, 'CATAT': 42084, 'CATAC': 53711, 'CATAG': 45652, 'CATTA': 58883, 'CATTT': 108171, 'CATTC': 108495, 'CATTG': 151814, 'CATCA': 299232, 'CATCT': 245410, 'CATCC': 318012, 'CATCG': 185624, 'CATGA': 211388, 'CATGT': 156915, 'CATGC': 193331, 'CATGG': 312372, 'CACAA': 159536, 'CACAT': 120666, 'CACAC': 183790, 'CACAG': 293888, 'CACTA': 78276, 'CACTT': 136097, 'CACTC': 147010, 'CACTG': 256902, 'CACCA': 380315, 'CACCT': 323507, 'CACCC': 400336, 'CACCG': 204786, 'CACGA': 101593, 'CACGT': 92145, 'CACGC': 199917, 'CACGG': 184821, 'CAGAA': 293767, 'CAGAT': 207076, 'CAGAC': 246612, 'CAGAG': 366817, 'CAGTA': 96746, 'CAGTT': 154192, 'CAGTC': 145568, 'CAGTG': 306480, 'CAGCA': 444026, 'CAGCT': 395602, 'CAGCC': 614765, 'CAGCG': 285261, 'CAGGA': 370762, 'CAGGT': 240656, 'CAGGC': 397400, 'CAGGG': 326889, 'CTAAA': 69867, 'CTAAT': 37996, 'CTAAC': 37043, 'CTAAG': 66245, 'CTATA': 39132, 'CTATT': 65164, 'CTATC': 71072, 'CTATG': 132966, 'CTACA': 193201, 'CTACT': 104400, 'CTACC': 217611, 'CTACG': 160540, 'CTAGA': 59366, 'CTAGT': 38328, 'CTAGC': 56245, 'CTAGG': 55778, 'CTTAA': 57999, 'CTTAT': 56084, 'CTTAC': 49880, 'CTTAG': 56695, 'CTTTA': 62526, 'CTTTT': 168675, 'CTTTC': 169910, 'CTTTG': 220589, 'CTTCA': 286029, 'CTTCT': 272984, 'CTTCC': 403562, 'CTTCG': 154631, 'CTTGA': 117061, 'CTTGT': 108976, 'CTTGC': 129999, 'CTTGG': 172916, 'CTCAA': 211628, 'CTCAT': 172545, 'CTCAC': 222973, 'CTCAG': 285993, 'CTCTA': 93022, 'CTCTT': 180254, 'CTCTC': 238020, 'CTCTG': 327446, 'CTCCA': 376915, 'CTCCT': 370484, 'CTCCC': 448920, 'CTCCG': 219308, 'CTCGA': 73712, 'CTCGT': 68637, 'CTCGC': 127560, 'CTCGG': 208089, 'CTGAA': 270103, 'CTGAT': 160849, 'CTGAC': 220411, 'CTGAG': 333093, 'CTGTA': 113160, 'CTGTT': 157852, 'CTGTC': 233665, 'CTGTG': 356375, 'CTGCA': 385139, 'CTGCT': 454267, 'CTGCC': 585833, 'CTGCG': 274378, 'CTGGA': 560593, 'CTGGT': 301566, 'CTGGC': 507818, 'CTGGG': 557134, 'CCAAA': 191950, 'CCAAT': 93408, 'CCAAC': 248547, 'CCAAG': 449385, 'CCATA': 67004, 'CCATT': 130591, 'CCATC': 348597, 'CCATG': 315300, 'CCACA': 281783, 'CCACT': 218161, 'CCACC': 530118, 'CCACG': 235005, 'CCAGA': 402490, 'CCAGT': 242019, 'CCAGC': 632679, 'CCAGG': 540116, 'CCTAA': 66486, 'CCTAT': 97647, 'CCTAC': 209801, 'CCTAG': 72674, 'CCTTA': 71326, 'CCTTT': 173673, 'CCTTC': 373671, 'CCTTG': 184817, 'CCTCA': 349390, 'CCTCT': 302876, 'CCTCC': 554024, 'CCTCG': 201022, 'CCTGA': 335546, 'CCTGT': 259545, 'CCTGC': 631737, 'CCTGG': 776327, 'CCCAA': 269803, 'CCCAT': 204113, 'CCCAC': 406384, 'CCCAG': 645350, 'CCCTA': 128910, 'CCCTT': 232672, 'CCCTC': 400114, 'CCCTG': 605840, 'CCCCA': 545034, 'CCCCT': 434633, 'CCCCC': 847762, 'CCCCG': 394126, 'CCCGA': 211266, 'CCCGT': 136553, 'CCCGC': 351969, 'CCCGG': 396866, 'CCGAA': 84605, 'CCGAT': 59285, 'CCGAC': 179865, 'CCGAG': 301948, 'CCGTA': 38509, 'CCGTT': 53868, 'CCGTC': 152923, 'CCGTG': 211728, 'CCGCA': 236795, 'CCGCT': 224660, 'CCGCC': 502807, 'CCGCG': 236082, 'CCGGA': 230297, 'CCGGT': 86384, 'CCGGC': 359654, 'CCGGG': 391502, 'CGAAA': 43503, 'CGAAT': 43151, 'CGAAC': 54445, 'CGAAG': 93526, 'CGATA': 23992, 'CGATT': 40439, 'CGATC': 43040, 'CGATG': 102988, 'CGACA': 132530, 'CGACT': 94199, 'CGACC': 163314, 'CGACG': 127030, 'CGAGA': 204456, 'CGAGT': 124114, 'CGAGC': 215892, 'CGAGG': 325457, 'CGTAA': 25442, 'CGTAT': 19207, 'CGTAC': 41501, 'CGTAG': 26933, 'CGTTA': 12615, 'CGTTT': 42843, 'CGTTC': 52884, 'CGTTG': 52719, 'CGTCA': 126913, 'CGTCT': 121607, 'CGTCC': 153165, 'CGTCG': 61422, 'CGTGA': 111364, 'CGTGT': 104380, 'CGTGC': 193985, 'CGTGG': 290547, 'CGCAA': 142749, 'CGCAT': 112742, 'CGCAC': 127940, 'CGCAG': 191105, 'CGCTA': 77669, 'CGCTT': 106384, 'CGCTC': 159045, 'CGCTG': 309863, 'CGCCA': 279395, 'CGCCT': 265478, 'CGCCC': 342732, 'CGCCG': 279970, 'CGCGA': 92874, 'CGCGT': 73184, 'CGCGC': 183532, 'CGCGG': 209426, 'CGGAA': 161451, 'CGGAT': 79651, 'CGGAC': 157079, 'CGGAG': 305923, 'CGGTA': 32196, 'CGGTT': 50055, 'CGGTC': 86485, 'CGGTG': 144587, 'CGGCA': 235211, 'CGGCT': 224561, 'CGGCC': 435334, 'CGGCG': 302135, 'CGGGA': 246746, 'CGGGT': 112922, 'CGGGC': 312081, 'CGGGG': 309881, 'GAAAA': 197019, 'GAAAT': 118841, 'GAAAC': 164068, 'GAAAG': 197992, 'GAATA': 62806, 'GAATT': 89560, 'GAATC': 82665, 'GAATG': 137884, 'GAACA': 186579, 'GAACT': 148722, 'GAACC': 188911, 'GAACG': 113493, 'GAAGA': 498388, 'GAAGT': 190913, 'GAAGC': 363471, 'GAAGG': 373928, 'GATAA': 56562, 'GATAT': 52549, 'GATAC': 48734, 'GATAG': 40469, 'GATTA': 52058, 'GATTT': 97932, 'GATTC': 107583, 'GATTG': 92355, 'GATCA': 158241, 'GATCT': 122260, 'GATCC': 207604, 'GATCG': 85246, 'GATGA': 314803, 'GATGT': 138180, 'GATGC': 182084, 'GATGG': 254438, 'GACAA': 229697, 'GACAT': 184024, 'GACAC': 168655, 'GACAG': 253330, 'GACTA': 72853, 'GACTT': 140469, 'GACTC': 182377, 'GACTG': 186836, 'GACCA': 325901, 'GACCT': 262172, 'GACCC': 330132, 'GACCG': 154499, 'GACGA': 155195, 'GACGT': 101721, 'GACGC': 173469, 'GACGG': 186609, 'GAGAA': 394582, 'GAGAT': 216968, 'GAGAC': 246759, 'GAGAG': 280694, 'GAGTA': 97558, 'GAGTT': 144790, 'GAGTC': 161147, 'GAGTG': 205849, 'GAGCA': 294355, 'GAGCT': 325477, 'GAGCC': 404977, 'GAGCG': 252535, 'GAGGA': 623151, 'GAGGT': 236558, 'GAGGC': 435408, 'GAGGG': 332954, 'GTAAA': 60030, 'GTAAT': 43682, 'GTAAC': 48299, 'GTAAG': 39215, 'GTATA': 25914, 'GTATT': 61271, 'GTATC': 48052, 'GTATG': 91541, 'GTACA': 111183, 'GTACT': 74984, 'GTACC': 126074, 'GTACG': 57236, 'GTAGA': 64732, 'GTAGT': 37770, 'GTAGC': 55964, 'GTAGG': 43520, 'GTTAA': 45817, 'GTTAT': 39047, 'GTTAC': 46742, 'GTTAG': 36704, 'GTTTA': 50960, 'GTTTT': 116976, 'GTTTC': 108615, 'GTTTG': 161550, 'GTTCA': 144992, 'GTTCT': 151623, 'GTTCC': 164439, 'GTTCG': 80998, 'GTTGA': 71824, 'GTTGT': 87184, 'GTTGC': 90097, 'GTTGG': 123837, 'GTCAA': 133278, 'GTCAT': 124663, 'GTCAC': 152246, 'GTCAG': 167859, 'GTCTA': 69139, 'GTCTT': 126968, 'GTCTC': 169864, 'GTCTG': 200826, 'GTCCA': 203730, 'GTCCT': 226933, 'GTCCC': 274003, 'GTCCG': 115212, 'GTCGA': 54589, 'GTCGT': 74856, 'GTCGC': 87462, 'GTCGG': 107066, 'GTGAA': 222249, 'GTGAT': 113715, 'GTGAC': 197057, 'GTGAG': 191315, 'GTGTA': 69843, 'GTGTT': 130314, 'GTGTC': 151795, 'GTGTG': 243161, 'GTGCA': 205694, 'GTGCT': 230434, 'GTGCC': 278500, 'GTGCG': 151405, 'GTGGA': 372516, 'GTGGT': 243020, 'GTGGC': 372769, 'GTGGG': 326973, 'GCAAA': 144790, 'GCAAT': 75511, 'GCAAC': 141271, 'GCAAG': 307914, 'GCATA': 32353, 'GCATT': 95601, 'GCATC': 211403, 'GCATG': 170832, 'GCACA': 175726, 'GCACT': 149753, 'GCACC': 305224, 'GCACG': 149693, 'GCAGA': 316611, 'GCAGT': 204540, 'GCAGC': 573530, 'GCAGG': 394034, 'GCTAA': 57429, 'GCTAT': 84072, 'GCTAC': 170150, 'GCTAG': 50044, 'GCTTA': 45796, 'GCTTT': 146172, 'GCTTC': 265398, 'GCTTG': 119570, 'GCTCA': 251510, 'GCTCT': 216433, 'GCTCC': 352385, 'GCTCG': 111705, 'GCTGA': 339855, 'GCTGT': 276585, 'GCTGC': 602177, 'GCTGG': 623470, 'GCCAA': 285195, 'GCCAT': 301990, 'GCCAC': 379447, 'GCCAG': 450244, 'GCCTA': 122486, 'GCCTT': 246292, 'GCCTC': 421117, 'GCCTG': 534662, 'GCCCA': 428987, 'GCCCT': 432789, 'GCCCC': 620338, 'GCCCG': 365673, 'GCCGA': 215819, 'GCCGT': 147593, 'GCCGC': 449123, 'GCCGG': 329467, 'GCGAA': 54579, 'GCGAT': 58389, 'GCGAC': 119233, 'GCGAG': 213536, 'GCGTA': 22225, 'GCGTT': 46910, 'GCGTC': 133403, 'GCGTG': 190011, 'GCGCA': 176885, 'GCGCT': 195652, 'GCGCC': 324172, 'GCGCG': 179887, 'GCGGA': 224486, 'GCGGT': 95993, 'GCGGC': 478365, 'GCGGG': 297659, 'GGAAA': 191158, 'GGAAT': 110396, 'GGAAC': 169383, 'GGAAG': 388380, 'GGATA': 58135, 'GGATT': 107066, 'GGATC': 135230, 'GGATG': 262881, 'GGACA': 317109, 'GGACT': 218732, 'GGACC': 378322, 'GGACG': 219258, 'GGAGA': 478317, 'GGAGT': 210657, 'GGAGC': 511969, 'GGAGG': 656813, 'GGTAA': 42893, 'GGTAT': 51384, 'GGTAC': 78193, 'GGTAG': 57134, 'GGTTA': 37031, 'GGTTT': 108057, 'GGTTC': 129567, 'GGTTG': 101715, 'GGTCA': 178664, 'GGTCT': 176104, 'GGTCC': 227862, 'GGTCG': 114071, 'GGTGA': 227685, 'GGTGT': 187397, 'GGTGC': 250254, 'GGTGG': 460669, 'GGCAA': 206143, 'GGCAT': 173131, 'GGCAC': 235556, 'GGCAG': 415955, 'GGCTA': 125189, 'GGCTT': 192272, 'GGCTC': 279378, 'GGCTG': 487090, 'GGCCA': 494142, 'GGCCT': 418738, 'GGCCC': 641984, 'GGCCG': 423628, 'GGCGA': 149785, 'GGCGT': 142525, 'GGCGC': 296973, 'GGCGG': 414274, 'GGGAA': 220551, 'GGGAT': 131701, 'GGGAC': 297309, 'GGGAG': 369810, 'GGGTA': 55568, 'GGGTT': 109027, 'GGGTC': 215153, 'GGGTG': 250278, 'GGGCA': 314640, 'GGGCT': 315705, 'GGGCC': 576546, 'GGGCG': 279356, 'GGGGA': 299383, 'GGGGT': 173267, 'GGGGC': 441456, 'GGGGG': 323962}, 'overrepresented_sequences': {}} {'total_reads': 3063108, 'total_bases': 177494975, 'q20_bases': 171681633, 'q30_bases': 164635590, 'total_cycles': 58, 'quality_curves': {'A': [37.0782, 37.0755, 37.0727, 37.0896, 37.0955, 37.1275, 37.0957, 37.0876, 37.094, 37.0913, 37.0767, 37.0476, 37.0111, 36.9404, 36.942, 36.9067, 36.8678, 36.8029, 36.8278, 36.8594, 36.8078, 36.7981, 36.7639, 36.6366, 36.5017, 36.3631, 36.2135, 36.1514, 35.9965, 35.9069, 35.8988, 35.7726, 35.7013, 35.6622, 35.6212, 35.6282, 35.462, 35.3974, 35.3487, 35.2872, 35.157, 35.0114, 35.005, 34.9741, 34.8966, 34.777, 34.7506, 34.6774, 34.6538, 34.4091, 34.3046, 34.1867, 34.105, 34.0664, 33.8399, 33.6962, 33.4997, 33.1621], 'T': [37.3979, 37.3752, 37.3941, 37.364, 37.3339, 37.3009, 37.2627, 37.2528, 37.2492, 37.2405, 37.2094, 37.1685, 37.1781, 37.1289, 37.1847, 37.2046, 37.1539, 37.0821, 37.0731, 37.0593, 37.0528, 37.0367, 37.0297, 36.9724, 36.8791, 36.7517, 36.6193, 36.5794, 36.4879, 36.4076, 36.3901, 36.291, 36.2236, 36.194, 36.1632, 36.1406, 36.0368, 35.9587, 35.8929, 35.8424, 35.7071, 35.5861, 35.5689, 35.5232, 35.4191, 35.3362, 35.2924, 35.2231, 35.1679, 34.9897, 34.801, 34.7162, 34.5805, 34.5643, 34.3423, 34.2197, 34.0703, 33.7278], 'C': [37.3454, 37.3707, 37.3409, 37.3363, 37.351, 37.3322, 37.3379, 37.3286, 37.3136, 37.3253, 37.3249, 37.3168, 37.2829, 37.2687, 37.2532, 37.2312, 37.2228, 37.2275, 37.2057, 37.2006, 37.1402, 37.1516, 37.116, 37.0183, 36.9156, 36.8329, 36.697, 36.6489, 36.5688, 36.4703, 36.4747, 36.4188, 36.3324, 36.3286, 36.3017, 36.2696, 36.2069, 36.1393, 36.0943, 36.0445, 35.9691, 35.8799, 35.846, 35.8288, 35.7504, 35.6521, 35.6238, 35.5832, 35.4986, 35.3769, 35.252, 35.126, 35.019, 34.9514, 34.814, 34.6699, 34.4762, 34.2052], 'G': [37.1137, 37.1071, 37.0871, 37.0898, 37.0916, 37.0989, 37.0801, 37.1049, 37.0971, 37.1121, 37.1095, 37.079, 37.0482, 36.9985, 36.9838, 36.949, 36.9402, 36.913, 36.9314, 36.9384, 36.8745, 36.8584, 36.8217, 36.7255, 36.6599, 36.5579, 36.4288, 36.3946, 36.3059, 36.2, 36.2188, 36.119, 36.0389, 36.0382, 36.0129, 36.0295, 35.9502, 35.8546, 35.8023, 35.7867, 35.6518, 35.5644, 35.5232, 35.5022, 35.4363, 35.3144, 35.2779, 35.1793, 35.1197, 34.997, 34.8164, 34.7104, 34.5918, 34.5655, 34.4005, 34.2291, 34.0121, 33.7437], 'mean': [37.2367, 37.2345, 37.2232, 37.2177, 37.2177, 37.2135, 37.1943, 37.1968, 37.1893, 37.1955, 37.1849, 37.158, 37.1339, 37.0909, 37.0947, 37.0751, 37.0518, 37.0155, 37.0188, 37.0231, 36.9745, 36.9674, 36.9391, 36.844, 36.7496, 36.6414, 36.5031, 36.4597, 36.3605, 36.2629, 36.266, 36.1745, 36.0949, 36.0806, 36.0528, 36.0413, 35.946, 35.8695, 35.8132, 35.7737, 35.658, 35.5466, 35.5226, 35.4987, 35.4137, 35.3079, 35.2777, 35.2027, 35.1463, 34.9885, 34.8349, 34.7272, 34.6173, 34.5754, 34.395, 34.2497, 34.0545, 33.7587]}, 'content_curves': {'A': [0.199085, 0.193293, 0.20129, 0.201281, 0.197627, 0.203204, 0.19913, 0.196379, 0.203486, 0.197412, 0.200475, 0.204949, 0.203096, 0.197218, 0.204918, 0.201042, 0.198274, 0.203826, 0.199941, 0.196475, 0.202025, 0.198858, 0.1962, 0.201333, 0.196413, 0.194939, 0.200561, 0.195371, 0.194244, 0.199527, 0.196121, 0.194559, 0.200362, 0.197195, 0.19367, 0.200261, 0.19707, 0.194654, 0.201649, 0.196207, 0.194586, 0.200159, 0.196396, 0.192685, 0.199226, 0.197846, 0.193565, 0.199322, 0.194042, 0.194534, 0.197873, 0.194294, 0.1949, 0.198883, 0.196815, 0.194624, 0.198698, 0.195478], 'T': [0.244321, 0.228197, 0.2349, 0.226716, 0.221822, 0.229963, 0.22547, 0.224467, 0.230824, 0.230414, 0.221904, 0.227809, 0.228132, 0.218776, 0.224352, 0.223403, 0.218987, 0.224656, 0.225543, 0.221419, 0.22459, 0.224902, 0.21982, 0.223876, 0.222622, 0.220146, 0.224846, 0.224834, 0.220918, 0.225448, 0.224011, 0.219816, 0.226225, 0.226509, 0.222678, 0.226666, 0.225003, 0.220645, 0.221774, 0.223796, 0.219971, 0.225132, 0.224955, 0.22203, 0.224318, 0.225729, 0.221082, 0.22592, 0.225908, 0.22235, 0.222199, 0.225306, 0.217398, 0.220823, 0.221806, 0.216887, 0.22194, 0.218622], 'C': [0.26168, 0.274242, 0.264359, 0.267002, 0.276006, 0.26722, 0.27136, 0.277564, 0.270346, 0.273532, 0.278559, 0.27358, 0.271253, 0.278829, 0.275977, 0.2747, 0.28023, 0.276514, 0.277586, 0.2802, 0.276395, 0.275722, 0.282185, 0.277195, 0.281316, 0.286419, 0.278267, 0.279634, 0.283516, 0.275962, 0.279795, 0.283822, 0.278949, 0.279896, 0.284863, 0.278676, 0.282459, 0.284315, 0.281676, 0.281432, 0.284666, 0.278821, 0.281698, 0.286737, 0.282629, 0.281107, 0.285422, 0.280981, 0.280149, 0.282817, 0.282793, 0.282174, 0.28753, 0.283656, 0.284698, 0.286624, 0.282807, 0.286369], 'G': [0.294914, 0.304268, 0.299446, 0.305001, 0.304545, 0.299612, 0.304041, 0.301591, 0.295321, 0.29863, 0.299055, 0.293662, 0.297519, 0.305177, 0.294752, 0.300854, 0.302508, 0.295004, 0.29693, 0.301906, 0.296991, 0.300518, 0.301795, 0.297595, 0.299649, 0.298496, 0.296325, 0.300161, 0.301323, 0.299063, 0.300074, 0.301804, 0.294463, 0.2964, 0.298789, 0.294397, 0.295468, 0.300386, 0.294901, 0.298565, 0.300776, 0.295889, 0.296948, 0.298547, 0.293828, 0.295317, 0.299931, 0.293777, 0.2999, 0.300299, 0.297135, 0.298226, 0.300171, 0.296638, 0.296681, 0.301865, 0.296555, 0.299529], 'N': [0, 0, 4.57052e-06, 0, 0, 0, 0, 0, 2.3832e-05, 1.10998e-05, 6.20285e-06, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 2.94046e-06, 1.30701e-06, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1.64486e-06], 'GC': [0.556594, 0.57851, 0.563805, 0.572003, 0.580551, 0.566832, 0.575401, 0.579155, 0.565666, 0.572163, 0.577615, 0.567242, 0.568772, 0.584006, 0.570729, 0.575555, 0.582739, 0.571519, 0.574515, 0.582106, 0.573385, 0.57624, 0.58398, 0.574791, 0.580965, 0.584915, 0.574593, 0.579795, 0.584839, 0.575025, 0.579869, 0.585625, 0.573412, 0.576296, 0.583652, 0.573073, 0.577927, 0.584701, 0.576577, 0.579996, 0.585443, 0.57471, 0.578646, 0.585284, 0.576456, 0.576424, 0.585353, 0.574759, 0.58005, 0.583116, 0.579928, 0.5804, 0.587701, 0.580294, 0.581379, 0.588489, 0.579362, 0.585898]}, 'kmer_count': {'AAAAA': 308815, 'AAAAT': 156458, 'AAAAC': 120459, 'AAAAG': 154972, 'AAATA': 136495, 'AAATT': 103865, 'AAATC': 98462, 'AAATG': 114750, 'AAACA': 148568, 'AAACT': 130436, 'AAACC': 109453, 'AAACG': 42569, 'AAAGA': 139507, 'AAAGT': 123860, 'AAAGC': 139796, 'AAAGG': 155368, 'AATAA': 107090, 'AATAT': 66509, 'AATAC': 56288, 'AATAG': 60714, 'AATTA': 59301, 'AATTT': 109532, 'AATTC': 87993, 'AATTG': 60063, 'AATCA': 80900, 'AATCT': 121105, 'AATCC': 98404, 'AATCG': 34164, 'AATGA': 97952, 'AATGT': 95784, 'AATGC': 95621, 'AATGG': 138077, 'AACAA': 104238, 'AACAT': 98945, 'AACAC': 114104, 'AACAG': 144331, 'AACTA': 36161, 'AACTT': 153639, 'AACTC': 133797, 'AACTG': 140215, 'AACCA': 99320, 'AACCT': 93463, 'AACCC': 103821, 'AACCG': 38892, 'AACGA': 23386, 'AACGT': 31479, 'AACGC': 39917, 'AACGG': 50411, 'AAGAA': 157523, 'AAGAT': 117149, 'AAGAC': 98467, 'AAGAG': 166656, 'AAGTA': 80696, 'AAGTT': 118626, 'AAGTC': 129500, 'AAGTG': 144733, 'AAGCA': 124518, 'AAGCT': 136368, 'AAGCC': 189653, 'AAGCG': 93217, 'AAGGA': 170455, 'AAGGT': 137105, 'AAGGC': 226040, 'AAGGG': 216057, 'ATAAA': 116606, 'ATAAT': 53126, 'ATAAC': 39460, 'ATAAG': 53344, 'ATATA': 52549, 'ATATT': 64751, 'ATATC': 42689, 'ATATG': 43849, 'ATACA': 80883, 'ATACT': 75485, 'ATACC': 43512, 'ATACG': 17442, 'ATAGA': 67055, 'ATAGT': 61909, 'ATAGC': 72995, 'ATAGG': 78652, 'ATTAA': 55909, 'ATTAT': 39907, 'ATTAC': 48697, 'ATTAG': 37261, 'ATTTA': 56828, 'ATTTT': 134608, 'ATTTC': 116355, 'ATTTG': 115811, 'ATTCA': 98139, 'ATTCT': 119975, 'ATTCC': 103956, 'ATTCG': 37209, 'ATTGA': 73297, 'ATTGT': 62192, 'ATTGC': 67760, 'ATTGG': 91077, 'ATCAA': 77634, 'ATCAT': 141643, 'ATCAC': 104915, 'ATCAG': 146627, 'ATCTA': 33612, 'ATCTT': 252816, 'ATCTC': 200182, 'ATCTG': 194320, 'ATCCA': 161957, 'ATCCT': 151995, 'ATCCC': 105966, 'ATCCG': 68779, 'ATCGA': 33074, 'ATCGT': 44603, 'ATCGC': 46271, 'ATCGG': 46175, 'ATGAA': 142205, 'ATGAT': 151448, 'ATGAC': 109123, 'ATGAG': 179018, 'ATGTA': 89528, 'ATGTT': 167770, 'ATGTC': 162822, 'ATGTG': 115205, 'ATGCA': 94470, 'ATGCT': 117048, 'ATGCC': 161257, 'ATGCG': 108155, 'ATGGA': 140706, 'ATGGT': 179946, 'ATGGC': 235297, 'ATGGG': 196309, 'ACAAA': 159253, 'ACAAT': 91523, 'ACAAC': 70480, 'ACAAG': 111228, 'ACATA': 74216, 'ACATT': 109690, 'ACATC': 125693, 'ACATG': 131505, 'ACACA': 173268, 'ACACT': 99888, 'ACACC': 162909, 'ACACG': 96110, 'ACAGA': 165312, 'ACAGT': 120149, 'ACAGC': 243367, 'ACAGG': 253048, 'ACTAA': 34880, 'ACTAT': 32936, 'ACTAC': 29450, 'ACTAG': 34082, 'ACTTA': 34365, 'ACTTT': 124387, 'ACTTC': 181738, 'ACTTG': 197999, 'ACTCA': 120713, 'ACTCT': 115477, 'ACTCC': 168714, 'ACTCG': 111499, 'ACTGA': 112372, 'ACTGT': 127553, 'ACTGC': 180712, 'ACTGG': 215475, 'ACCAA': 82285, 'ACCAT': 123219, 'ACCAC': 215866, 'ACCAG': 267394, 'ACCTA': 29155, 'ACCTT': 155948, 'ACCTC': 204548, 'ACCTG': 204750, 'ACCCA': 160654, 'ACCCT': 133138, 'ACCCC': 152267, 'ACCCG': 88388, 'ACCGA': 55931, 'ACCGT': 59092, 'ACCGC': 77581, 'ACCGG': 69024, 'ACGAA': 51039, 'ACGAT': 57754, 'ACGAC': 50696, 'ACGAG': 68192, 'ACGTA': 33968, 'ACGTT': 78232, 'ACGTC': 91751, 'ACGTG': 86436, 'ACGCA': 68936, 'ACGCT': 73144, 'ACGCC': 113816, 'ACGCG': 54153, 'ACGGA': 76939, 'ACGGT': 85475, 'ACGGC': 117841, 'ACGGG': 118484, 'AGAAA': 177298, 'AGAAT': 91417, 'AGAAC': 135826, 'AGAAG': 257363, 'AGATA': 75740, 'AGATT': 71615, 'AGATC': 109750, 'AGATG': 210313, 'AGACA': 134833, 'AGACT': 88556, 'AGACC': 148893, 'AGACG': 94330, 'AGAGA': 188354, 'AGAGT': 104960, 'AGAGC': 183541, 'AGAGG': 303455, 'AGTAA': 66937, 'AGTAT': 51571, 'AGTAC': 66409, 'AGTAG': 97213, 'AGTTA': 51046, 'AGTTT': 124944, 'AGTTC': 129501, 'AGTTG': 131462, 'AGTCA': 121235, 'AGTCT': 132079, 'AGTCC': 187303, 'AGTCG': 81749, 'AGTGA': 141844, 'AGTGT': 109539, 'AGTGC': 142218, 'AGTGG': 216956, 'AGCAA': 110524, 'AGCAT': 128714, 'AGCAC': 209862, 'AGCAG': 368555, 'AGCTA': 53405, 'AGCTT': 218081, 'AGCTC': 283398, 'AGCTG': 370062, 'AGCCA': 270307, 'AGCCT': 220799, 'AGCCC': 291278, 'AGCCG': 187285, 'AGCGA': 77758, 'AGCGT': 114703, 'AGCGC': 158295, 'AGCGG': 188435, 'AGGAA': 275488, 'AGGAT': 180388, 'AGGAC': 204370, 'AGGAG': 365964, 'AGGTA': 124340, 'AGGTT': 143871, 'AGGTC': 232703, 'AGGTG': 321886, 'AGGCA': 279639, 'AGGCT': 292077, 'AGGCC': 358609, 'AGGCG': 228364, 'AGGGA': 256025, 'AGGGT': 215494, 'AGGGC': 392446, 'AGGGG': 429819, 'TAAAA': 130666, 'TAAAT': 78711, 'TAAAC': 54959, 'TAAAG': 62209, 'TAATA': 42917, 'TAATT': 60539, 'TAATC': 55897, 'TAATG': 60604, 'TAACA': 60921, 'TAACT': 58320, 'TAACC': 36250, 'TAACG': 10480, 'TAAGA': 55973, 'TAAGT': 40315, 'TAAGC': 46671, 'TAAGG': 69171, 'TATAA': 42264, 'TATAT': 43473, 'TATAC': 28246, 'TATAG': 38518, 'TATTA': 31105, 'TATTT': 110293, 'TATTC': 60519, 'TATTG': 62273, 'TATCA': 51562, 'TATCT': 61138, 'TATCC': 52110, 'TATCG': 20790, 'TATGA': 43565, 'TATGT': 43069, 'TATGC': 31255, 'TATGG': 65552, 'TACAA': 73028, 'TACAT': 67979, 'TACAC': 72832, 'TACAG': 108825, 'TACTA': 19712, 'TACTT': 108510, 'TACTC': 81069, 'TACTG': 84686, 'TACCA': 59723, 'TACCT': 48770, 'TACCC': 53529, 'TACCG': 28150, 'TACGA': 17170, 'TACGT': 24468, 'TACGC': 18856, 'TACGG': 37939, 'TAGAA': 86799, 'TAGAT': 69065, 'TAGAC': 54995, 'TAGAG': 93672, 'TAGTA': 53673, 'TAGTT': 62460, 'TAGTC': 69427, 'TAGTG': 75013, 'TAGCA': 65710, 'TAGCT': 76901, 'TAGCC': 100877, 'TAGCG': 59202, 'TAGGA': 97681, 'TAGGT': 88898, 'TAGGC': 96814, 'TAGGG': 113940, 'TTAAA': 101600, 'TTAAT': 70556, 'TTAAC': 46285, 'TTAAG': 59828, 'TTATA': 42259, 'TTATT': 76597, 'TTATC': 51011, 'TTATG': 46870, 'TTACA': 75865, 'TTACT': 43128, 'TTACC': 37046, 'TTACG': 24545, 'TTAGA': 34690, 'TTAGT': 48987, 'TTAGC': 46168, 'TTAGG': 66291, 'TTTAA': 102558, 'TTTAT': 90253, 'TTTAC': 57807, 'TTTAG': 56056, 'TTTTA': 101971, 'TTTTT': 344088, 'TTTTC': 195512, 'TTTTG': 147397, 'TTTCA': 144470, 'TTTCT': 281653, 'TTTCC': 184492, 'TTTCG': 34741, 'TTTGA': 119389, 'TTTGT': 172469, 'TTTGC': 137847, 'TTTGG': 165753, 'TTCAA': 132093, 'TTCAT': 178629, 'TTCAC': 208230, 'TTCAG': 248511, 'TTCTA': 65995, 'TTCTT': 420539, 'TTCTC': 369844, 'TTCTG': 282132, 'TTCCA': 195471, 'TTCCT': 257977, 'TTCCC': 203818, 'TTCCG': 123319, 'TTCGA': 44687, 'TTCGT': 35561, 'TTCGC': 38984, 'TTCGG': 62312, 'TTGAA': 163707, 'TTGAT': 181267, 'TTGAC': 134278, 'TTGAG': 199981, 'TTGTA': 135418, 'TTGTT': 183078, 'TTGTC': 212064, 'TTGTG': 147493, 'TTGCA': 129509, 'TTGCT': 175562, 'TTGCC': 182078, 'TTGCG': 119831, 'TTGGA': 188343, 'TTGGT': 207751, 'TTGGC': 266457, 'TTGGG': 255072, 'TCAAA': 154256, 'TCAAT': 124125, 'TCAAC': 69499, 'TCAAG': 105973, 'TCATA': 91445, 'TCATT': 108035, 'TCATC': 279514, 'TCATG': 191736, 'TCACA': 185476, 'TCACT': 166968, 'TCACC': 205115, 'TCACG': 101100, 'TCAGA': 149572, 'TCAGT': 143080, 'TCAGC': 300428, 'TCAGG': 302416, 'TCTAA': 43445, 'TCTAT': 50894, 'TCTAC': 58687, 'TCTAG': 58644, 'TCTTA': 69290, 'TCTTT': 221121, 'TCTTC': 451877, 'TCTTG': 419828, 'TCTCA': 163078, 'TCTCT': 246931, 'TCTCC': 421425, 'TCTCG': 184788, 'TCTGA': 160406, 'TCTGT': 192136, 'TCTGC': 293685, 'TCTGG': 388366, 'TCCAA': 133853, 'TCCAT': 180078, 'TCCAC': 325207, 'TCCAG': 497396, 'TCCTA': 55538, 'TCCTT': 325145, 'TCCTC': 553384, 'TCCTG': 340683, 'TCCCA': 241583, 'TCCCT': 168981, 'TCCCC': 254381, 'TCCCG': 206746, 'TCCGA': 78409, 'TCCGT': 115721, 'TCCGC': 155295, 'TCCGG': 186024, 'TCGAA': 96259, 'TCGAT': 90312, 'TCGAC': 37090, 'TCGAG': 58244, 'TCGTA': 76118, 'TCGTT': 68734, 'TCGTC': 140375, 'TCGTG': 94961, 'TCGCA': 75610, 'TCGCT': 97775, 'TCGCC': 117835, 'TCGCG': 67285, 'TCGGA': 82778, 'TCGGT': 82728, 'TCGGC': 168373, 'TCGGG': 182960, 'TGAAA': 112239, 'TGAAT': 90297, 'TGAAC': 129505, 'TGAAG': 276450, 'TGATA': 56243, 'TGATT': 62766, 'TGATC': 147677, 'TGATG': 288794, 'TGACA': 137728, 'TGACT': 104465, 'TGACC': 167865, 'TGACG': 125674, 'TGAGA': 168000, 'TGAGT': 118288, 'TGAGC': 245930, 'TGAGG': 328657, 'TGTAA': 75219, 'TGTAT': 64557, 'TGTAC': 99121, 'TGTAG': 180877, 'TGTTA': 63685, 'TGTTT': 149526, 'TGTTC': 175623, 'TGTTG': 246902, 'TGTCA': 173414, 'TGTCT': 195221, 'TGTCC': 292723, 'TGTCG': 116505, 'TGTGA': 126837, 'TGTGT': 157051, 'TGTGC': 168568, 'TGTGG': 273197, 'TGCAA': 102919, 'TGCAT': 129114, 'TGCAC': 175427, 'TGCAG': 338165, 'TGCTA': 47983, 'TGCTT': 202854, 'TGCTC': 266226, 'TGCTG': 419035, 'TGCCA': 228358, 'TGCCT': 212337, 'TGCCC': 286422, 'TGCCG': 201581, 'TGCGA': 47994, 'TGCGT': 80044, 'TGCGC': 143591, 'TGCGG': 226731, 'TGGAA': 219553, 'TGGAT': 158567, 'TGGAC': 181740, 'TGGAG': 366503, 'TGGTA': 112391, 'TGGTT': 166472, 'TGGTC': 286546, 'TGGTG': 357317, 'TGGCA': 282260, 'TGGCT': 305023, 'TGGCC': 436358, 'TGGCG': 225566, 'TGGGA': 268408, 'TGGGT': 261947, 'TGGGC': 392389, 'TGGGG': 553750, 'CAAAA': 164775, 'CAAAT': 100112, 'CAAAC': 151612, 'CAAAG': 194353, 'CAATA': 55470, 'CAATT': 69255, 'CAATC': 88856, 'CAATG': 147645, 'CAACA': 115053, 'CAACT': 85891, 'CAACC': 84229, 'CAACG': 44184, 'CAAGA': 105258, 'CAAGT': 109708, 'CAAGC': 104330, 'CAAGG': 181926, 'CATAA': 62976, 'CATAT': 48933, 'CATAC': 86140, 'CATAG': 123594, 'CATTA': 58124, 'CATTT': 112915, 'CATTC': 135198, 'CATTG': 115598, 'CATCA': 209101, 'CATCT': 262053, 'CATCC': 221683, 'CATCG': 81986, 'CATGA': 186666, 'CATGT': 165346, 'CATGC': 159867, 'CATGG': 248683, 'CACAA': 160917, 'CACAT': 163835, 'CACAC': 209775, 'CACAG': 319048, 'CACTA': 45333, 'CACTT': 146526, 'CACTC': 164274, 'CACTG': 288060, 'CACCA': 342479, 'CACCT': 297196, 'CACCC': 218537, 'CACCG': 118261, 'CACGA': 111418, 'CACGT': 146992, 'CACGC': 154724, 'CACGG': 186740, 'CAGAA': 211626, 'CAGAT': 153194, 'CAGAC': 162655, 'CAGAG': 287606, 'CAGTA': 86668, 'CAGTT': 147187, 'CAGTC': 166535, 'CAGTG': 235244, 'CAGCA': 431907, 'CAGCT': 463028, 'CAGCC': 424000, 'CAGCG': 248781, 'CAGGA': 427001, 'CAGGT': 357788, 'CAGGC': 470800, 'CAGGG': 563164, 'CTAAA': 46869, 'CTAAT': 31162, 'CTAAC': 36527, 'CTAAG': 47592, 'CTATA': 21849, 'CTATT': 48795, 'CTATC': 42141, 'CTATG': 43342, 'CTACA': 55661, 'CTACT': 47117, 'CTACC': 45407, 'CTACG': 24193, 'CTAGA': 59693, 'CTAGT': 23079, 'CTAGC': 44864, 'CTAGG': 74134, 'CTTAA': 65610, 'CTTAT': 40600, 'CTTAC': 31756, 'CTTAG': 62302, 'CTTTA': 100850, 'CTTTT': 170737, 'CTTTC': 175651, 'CTTTG': 199362, 'CTTCA': 357269, 'CTTCT': 502844, 'CTTCC': 347812, 'CTTCG': 68288, 'CTTGA': 302013, 'CTTGT': 267905, 'CTTGC': 273223, 'CTTGG': 421592, 'CTCAA': 135871, 'CTCAT': 205357, 'CTCAC': 157299, 'CTCAG': 299607, 'CTCTA': 66428, 'CTCTT': 268174, 'CTCTC': 227300, 'CTCTG': 335973, 'CTCCA': 492851, 'CTCCT': 557154, 'CTCCC': 296473, 'CTCCG': 216238, 'CTCGA': 124295, 'CTCGT': 186767, 'CTCGC': 180863, 'CTCGG': 256757, 'CTGAA': 151519, 'CTGAT': 99571, 'CTGAC': 146435, 'CTGAG': 264709, 'CTGTA': 109427, 'CTGTT': 157526, 'CTGTC': 237540, 'CTGTG': 276975, 'CTGCA': 347084, 'CTGCT': 411701, 'CTGCC': 380176, 'CTGCG': 163437, 'CTGGA': 341499, 'CTGGT': 319880, 'CTGGC': 401174, 'CTGGG': 609874, 'CCAAA': 168593, 'CCAAT': 67688, 'CCAAC': 107359, 'CCAAG': 165429, 'CCATA': 85501, 'CCATT': 117332, 'CCATC': 206198, 'CCATG': 264352, 'CCACA': 287176, 'CCACT': 200614, 'CCACC': 396017, 'CCACG': 253365, 'CCAGA': 259940, 'CCAGT': 198906, 'CCAGC': 536730, 'CCAGG': 682767, 'CCTAA': 38071, 'CCTAT': 43543, 'CCTAC': 35218, 'CCTAG': 53647, 'CCTTA': 45887, 'CCTTT': 146902, 'CCTTC': 334422, 'CCTTG': 369800, 'CCTCA': 276499, 'CCTCT': 300628, 'CCTCC': 562429, 'CCTCG': 272027, 'CCTGA': 175026, 'CCTGT': 186811, 'CCTGC': 347162, 'CCTGG': 493342, 'CCCAA': 147000, 'CCCAT': 177333, 'CCCAC': 280947, 'CCCAG': 478982, 'CCCTA': 45325, 'CCCTT': 193925, 'CCCTC': 277625, 'CCCTG': 299578, 'CCCCA': 326032, 'CCCCT': 217437, 'CCCCC': 267615, 'CCCCG': 239998, 'CCCGA': 121736, 'CCCGT': 131714, 'CCCGC': 222345, 'CCCGG': 308465, 'CCGAA': 72445, 'CCGAT': 67626, 'CCGAC': 76571, 'CCGAG': 154617, 'CCGTA': 66863, 'CCGTT': 88995, 'CCGTC': 156108, 'CCGTG': 165161, 'CCGCA': 153981, 'CCGCT': 207230, 'CCGCC': 276132, 'CCGCG': 143980, 'CCGGA': 142478, 'CCGGT': 141335, 'CCGGC': 263875, 'CCGGG': 315511, 'CGAAA': 39467, 'CGAAT': 36350, 'CGAAC': 58533, 'CGAAG': 135579, 'CGATA': 18770, 'CGATT': 28097, 'CGATC': 75973, 'CGATG': 166511, 'CGACA': 53838, 'CGACT': 58557, 'CGACC': 69498, 'CGACG': 44456, 'CGAGA': 75899, 'CGAGT': 61730, 'CGAGC': 95940, 'CGAGG': 166853, 'CGTAA': 19314, 'CGTAT': 23751, 'CGTAC': 50465, 'CGTAG': 126475, 'CGTTA': 16479, 'CGTTT': 65334, 'CGTTC': 108421, 'CGTTG': 122150, 'CGTCA': 95864, 'CGTCT': 135165, 'CGTCC': 178757, 'CGTCG': 116932, 'CGTGA': 86939, 'CGTGT': 94545, 'CGTGC': 128258, 'CGTGG': 204348, 'CGCAA': 37034, 'CGCAT': 61334, 'CGCAC': 114849, 'CGCAG': 239466, 'CGCTA': 17857, 'CGCTT': 115300, 'CGCTC': 195720, 'CGCTG': 221256, 'CGCCA': 159413, 'CGCCT': 157466, 'CGCCC': 222934, 'CGCCG': 186635, 'CGCGA': 51347, 'CGCGT': 59021, 'CGCGC': 116759, 'CGCGG': 172796, 'CGGAA': 93057, 'CGGAT': 113315, 'CGGAC': 93176, 'CGGAG': 166548, 'CGGTA': 80729, 'CGGTT': 76983, 'CGGTC': 123715, 'CGGTG': 190263, 'CGGCA': 163267, 'CGGCT': 188541, 'CGGCC': 316550, 'CGGCG': 216726, 'CGGGA': 168567, 'CGGGT': 120846, 'CGGGC': 303154, 'CGGGG': 324742, 'GAAAA': 133135, 'GAAAT': 115189, 'GAAAC': 103508, 'GAAAG': 146946, 'GAATA': 56055, 'GAATT': 82362, 'GAATC': 90070, 'GAATG': 102695, 'GAACA': 137198, 'GAACT': 189024, 'GAACC': 106337, 'GAACG': 47608, 'GAAGA': 239876, 'GAAGT': 199285, 'GAAGC': 252372, 'GAAGG': 343941, 'GATAA': 50073, 'GATAT': 45317, 'GATAC': 46760, 'GATAG': 58033, 'GATTA': 31815, 'GATTT': 89265, 'GATTC': 75030, 'GATTG': 55557, 'GATCA': 128320, 'GATCT': 234066, 'GATCC': 117762, 'GATCG': 33325, 'GATGA': 252240, 'GATGT': 229287, 'GATGC': 193623, 'GATGG': 299588, 'GACAA': 92929, 'GACAT': 110881, 'GACAC': 135870, 'GACAG': 210425, 'GACTA': 29742, 'GACTT': 130639, 'GACTC': 139541, 'GACTG': 122586, 'GACCA': 186743, 'GACCT': 157398, 'GACCC': 161639, 'GACCG': 76161, 'GACGA': 76459, 'GACGT': 87879, 'GACGC': 97335, 'GACGG': 124331, 'GAGAA': 205877, 'GAGAT': 128072, 'GAGAC': 151560, 'GAGAG': 233016, 'GAGTA': 60102, 'GAGTT': 108785, 'GAGTC': 156500, 'GAGTG': 153928, 'GAGCA': 196884, 'GAGCT': 252375, 'GAGCC': 258632, 'GAGCG': 139107, 'GAGGA': 329046, 'GAGGT': 236294, 'GAGGC': 366512, 'GAGGG': 398113, 'GTAAA': 61049, 'GTAAT': 64722, 'GTAAC': 42777, 'GTAAG': 50705, 'GTATA': 35191, 'GTATT': 71148, 'GTATC': 48851, 'GTATG': 48724, 'GTACA': 109916, 'GTACT': 128472, 'GTACC': 64450, 'GTACG': 32934, 'GTAGA': 143644, 'GTAGT': 125697, 'GTAGC': 138288, 'GTAGG': 177266, 'GTTAA': 52788, 'GTTAT': 44115, 'GTTAC': 41930, 'GTTAG': 38687, 'GTTTA': 45118, 'GTTTT': 137080, 'GTTTC': 156079, 'GTTTG': 130416, 'GTTCA': 166252, 'GTTCT': 231286, 'GTTCC': 144006, 'GTTCG': 41983, 'GTTGA': 182274, 'GTTGT': 173590, 'GTTGC': 126566, 'GTTGG': 237722, 'GTCAA': 107287, 'GTCAT': 144068, 'GTCAC': 186361, 'GTCAG': 199882, 'GTCTA': 44709, 'GTCTT': 218605, 'GTCTC': 217674, 'GTCTG': 220360, 'GTCCA': 284607, 'GTCCT': 305736, 'GTCCC': 265854, 'GTCCG': 126707, 'GTCGA': 80034, 'GTCGT': 113178, 'GTCGC': 92931, 'GTCGG': 150742, 'GTGAA': 149351, 'GTGAT': 120837, 'GTGAC': 144940, 'GTGAG': 216415, 'GTGTA': 84376, 'GTGTT': 126438, 'GTGTC': 162816, 'GTGTG': 182062, 'GTGCA': 174458, 'GTGCT': 231388, 'GTGCC': 205974, 'GTGCG': 106044, 'GTGGA': 253611, 'GTGGT': 212918, 'GTGGC': 346496, 'GTGGG': 413348, 'GCAAA': 129970, 'GCAAT': 78264, 'GCAAC': 82993, 'GCAAG': 117557, 'GCATA': 72668, 'GCATT': 86433, 'GCATC': 165479, 'GCATG': 171935, 'GCACA': 209293, 'GCACT': 177153, 'GCACC': 215337, 'GCACG': 150464, 'GCAGA': 240968, 'GCAGT': 173713, 'GCAGC': 493685, 'GCAGG': 581414, 'GCTAA': 45383, 'GCTAT': 28953, 'GCTAC': 49417, 'GCTAG': 55528, 'GCTTA': 51203, 'GCTTT': 152408, 'GCTTC': 310781, 'GCTTG': 276036, 'GCTCA': 237658, 'GCTCT': 236988, 'GCTCC': 414932, 'GCTCG': 183733, 'GCTGA': 215935, 'GCTGT': 274267, 'GCTGC': 483791, 'GCTGG': 576629, 'GCCAA': 146681, 'GCCAT': 192412, 'GCCAC': 318193, 'GCCAG': 434547, 'GCCTA': 40466, 'GCCTT': 223354, 'GCCTC': 375133, 'GCCTG': 356926, 'GCCCA': 358989, 'GCCCT': 297698, 'GCCCC': 382013, 'GCCCG': 252286, 'GCCGA': 116471, 'GCCGT': 170442, 'GCCGC': 327599, 'GCCGG': 299778, 'GCGAA': 51088, 'GCGAT': 73720, 'GCGAC': 62941, 'GCGAG': 120455, 'GCGTA': 43014, 'GCGTT': 75381, 'GCGTC': 140492, 'GCGTG': 167595, 'GCGCA': 154454, 'GCGCT': 174461, 'GCGCC': 221377, 'GCGCG': 136856, 'GCGGA': 164043, 'GCGGT': 163425, 'GCGGC': 337819, 'GCGGG': 301719, 'GGAAA': 169024, 'GGAAT': 113241, 'GGAAC': 157260, 'GGAAG': 367471, 'GGATA': 49517, 'GGATT': 87584, 'GGATC': 179178, 'GGATG': 308780, 'GGACA': 222356, 'GGACT': 172761, 'GGACC': 197172, 'GGACG': 121631, 'GGAGA': 287693, 'GGAGT': 194540, 'GGAGC': 323226, 'GGAGG': 530249, 'GGTAA': 57414, 'GGTAT': 62985, 'GGTAC': 119790, 'GGTAG': 180586, 'GGTTA': 45350, 'GGTTT': 128299, 'GGTTC': 171664, 'GGTTG': 218111, 'GGTCA': 245085, 'GGTCT': 240152, 'GGTCC': 322308, 'GGTCG': 120870, 'GGTGA': 273852, 'GGTGT': 191423, 'GGTGC': 277734, 'GGTGG': 528975, 'GGCAA': 158195, 'GGCAT': 177010, 'GGCAC': 255061, 'GGCAG': 547003, 'GGCTA': 60461, 'GGCTT': 257146, 'GGCTC': 330497, 'GGCTG': 537853, 'GGCCA': 438134, 'GGCCT': 408189, 'GGCCC': 492195, 'GGCCG': 341858, 'GGCGA': 131041, 'GGCGT': 173585, 'GGCGC': 269105, 'GGCGG': 379707, 'GGGAA': 218594, 'GGGAT': 170997, 'GGGAC': 235933, 'GGGAG': 437500, 'GGGTA': 103121, 'GGGTT': 174017, 'GGGTC': 288241, 'GGGTG': 395556, 'GGGCA': 410725, 'GGGCT': 400794, 'GGGCC': 572532, 'GGGCG': 283517, 'GGGGA': 367770, 'GGGGT': 361168, 'GGGGC': 580596, 'GGGGG': 524802}, 'overrepresented_sequences': {}} {'total_reads': 3132690, 'total_bases': 197334246, 'q20_bases': 187886928, 'q30_bases': 179071763, 'total_cycles': 63, 'quality_curves': {'A': [37.0162, 37.0118, 37.009, 37.0259, 37.0288, 37.0574, 37.0229, 37.015, 37.0165, 37.013, 36.9945, 36.9586, 36.916, 36.8357, 36.8352, 36.7971, 36.7542, 36.6867, 36.7084, 36.7276, 36.6793, 36.665, 36.6286, 36.5049, 36.3679, 36.2244, 36.0754, 36.0076, 35.848, 35.7582, 35.7407, 35.6077, 35.5375, 35.4871, 35.4412, 35.4447, 35.2727, 35.2048, 35.1568, 35.0944, 34.9585, 34.8124, 34.8116, 34.7685, 34.6939, 34.5695, 34.5325, 34.4545, 34.4274, 34.1786, 34.0641, 33.939, 33.859, 33.8169, 33.5937, 33.4424, 33.244, 32.9006, 32.8205, 32.2639, 31.83, 30.7459, 18.0288], 'T': [37.3418, 37.3154, 37.3345, 37.2987, 37.2667, 37.2294, 37.185, 37.1684, 37.1647, 37.1563, 37.1205, 37.0753, 37.0835, 37.0292, 37.0844, 37.1026, 37.0489, 36.9765, 36.9681, 36.9516, 36.9366, 36.9158, 36.8974, 36.8236, 36.7162, 36.5763, 36.4401, 36.3971, 36.2962, 36.2101, 36.1899, 36.0768, 36.0087, 35.9702, 35.9238, 35.9007, 35.7903, 35.6958, 35.6286, 35.5742, 35.4321, 35.3078, 35.2878, 35.2408, 35.1359, 35.0544, 35.0108, 34.9477, 34.8981, 34.7215, 34.5267, 34.452, 34.3084, 34.2923, 34.0734, 33.9439, 33.8, 33.447, 33.3259, 32.8008, 32.4147, 31.4949, 19.9523], 'C': [37.2829, 37.3081, 37.2754, 37.2717, 37.2858, 37.2652, 37.2672, 37.2588, 37.2421, 37.2498, 37.2484, 37.2376, 37.1956, 37.1769, 37.1603, 37.135, 37.1234, 37.1268, 37.1054, 37.0983, 37.0355, 37.0407, 37.0062, 36.9094, 36.8034, 36.7212, 36.5781, 36.524, 36.4408, 36.3405, 36.337, 36.2785, 36.1837, 36.1744, 36.1395, 36.1024, 36.0334, 35.9643, 35.9185, 35.8557, 35.7828, 35.6896, 35.6477, 35.6332, 35.5511, 35.4489, 35.4144, 35.371, 35.2804, 35.1535, 35.0287, 34.8948, 34.7919, 34.7149, 34.5643, 34.4187, 34.2185, 33.9406, 33.7222, 33.3403, 32.8285, 31.8367, 23.8476], 'G': [37.0355, 37.028, 37.0087, 37.0105, 37.01, 37.0178, 36.996, 37.018, 37.0061, 37.0169, 37.0147, 36.9773, 36.9348, 36.8807, 36.8601, 36.8232, 36.8124, 36.7786, 36.7936, 36.8003, 36.7332, 36.7208, 36.6782, 36.5802, 36.5156, 36.413, 36.2778, 36.2403, 36.148, 36.0367, 36.0531, 35.9517, 35.8588, 35.8554, 35.8266, 35.8351, 35.7509, 35.6551, 35.5902, 35.5759, 35.4361, 35.3522, 35.3043, 35.2823, 35.2077, 35.0792, 35.0393, 34.9354, 34.8711, 34.7467, 34.5657, 34.451, 34.327, 34.2967, 34.1296, 33.9556, 33.7284, 33.4557, 33.2533, 32.7868, 32.1623, 31.339, 23.7179], 'mean': [37.1713, 37.1674, 37.1557, 37.1487, 37.1468, 37.1406, 37.1176, 37.118, 37.1078, 37.1115, 37.099, 37.0669, 37.0356, 36.9868, 36.9881, 36.966, 36.9397, 36.9005, 36.9024, 36.9029, 36.8514, 36.8417, 36.8091, 36.7107, 36.6122, 36.5004, 36.3573, 36.3094, 36.2055, 36.1041, 36.1019, 36.005, 35.919, 35.8981, 35.8628, 35.8464, 35.7454, 35.6644, 35.6039, 35.5599, 35.4411, 35.3283, 35.3, 35.2744, 35.1863, 35.0767, 35.042, 34.9647, 34.9058, 34.7456, 34.5886, 34.4769, 34.3653, 34.3186, 34.1356, 33.9856, 33.7867, 33.4842, 33.3202, 32.8422, 32.3424, 31.4008, 21.8563]}, 'content_curves': {'A': [0.19876, 0.19306, 0.201026, 0.200996, 0.197357, 0.202967, 0.198867, 0.196069, 0.203222, 0.197173, 0.20022, 0.204693, 0.202844, 0.197043, 0.204664, 0.200873, 0.198097, 0.203664, 0.199756, 0.196345, 0.201963, 0.198712, 0.19616, 0.201153, 0.196111, 0.194663, 0.200194, 0.19503, 0.193939, 0.199204, 0.195846, 0.194314, 0.20006, 0.196898, 0.193369, 0.199898, 0.196786, 0.194325, 0.201259, 0.195872, 0.194207, 0.199797, 0.195988, 0.192373, 0.198816, 0.197542, 0.193288, 0.19905, 0.193759, 0.194296, 0.197791, 0.194125, 0.19487, 0.199075, 0.196416, 0.194755, 0.198265, 0.195377, 0.193771, 0.199438, 0.194609, 0.193171, 0.188726], 'T': [0.2448, 0.22867, 0.235451, 0.22725, 0.222312, 0.230478, 0.225931, 0.224953, 0.231217, 0.230724, 0.222122, 0.22803, 0.228199, 0.218831, 0.224417, 0.22341, 0.218992, 0.22462, 0.22539, 0.221247, 0.224464, 0.224799, 0.21975, 0.223984, 0.222906, 0.220405, 0.225214, 0.225056, 0.221249, 0.225771, 0.224233, 0.220115, 0.226467, 0.226625, 0.222934, 0.226886, 0.225209, 0.220925, 0.22198, 0.22404, 0.220245, 0.225311, 0.225122, 0.22221, 0.224573, 0.225891, 0.221229, 0.22598, 0.225955, 0.22239, 0.222226, 0.225252, 0.217388, 0.220835, 0.221535, 0.216685, 0.222222, 0.218363, 0.216103, 0.218807, 0.221573, 0.215101, 0.207696], 'C': [0.261433, 0.27395, 0.264004, 0.266658, 0.275611, 0.266898, 0.270996, 0.277234, 0.270046, 0.273203, 0.278253, 0.273308, 0.271101, 0.278584, 0.275754, 0.274496, 0.280069, 0.276284, 0.277402, 0.279991, 0.276164, 0.275529, 0.281891, 0.276857, 0.280915, 0.285973, 0.277892, 0.279335, 0.283159, 0.275592, 0.279457, 0.283457, 0.278616, 0.279608, 0.284568, 0.278436, 0.282214, 0.284035, 0.281343, 0.281191, 0.284408, 0.278618, 0.281527, 0.286444, 0.282311, 0.280747, 0.285157, 0.280696, 0.279901, 0.282556, 0.282457, 0.281852, 0.287171, 0.283128, 0.284512, 0.286162, 0.282409, 0.286423, 0.288038, 0.282994, 0.283514, 0.286955, 0.293782], 'G': [0.295008, 0.30432, 0.299514, 0.305095, 0.304721, 0.299656, 0.304205, 0.301744, 0.295492, 0.298888, 0.2994, 0.293968, 0.297856, 0.305541, 0.295165, 0.30122, 0.302841, 0.295432, 0.297451, 0.302418, 0.297408, 0.30096, 0.3022, 0.298006, 0.300068, 0.298959, 0.2967, 0.300579, 0.301653, 0.299433, 0.300464, 0.302113, 0.294857, 0.296869, 0.29913, 0.29478, 0.29579, 0.300715, 0.295417, 0.298897, 0.30114, 0.296274, 0.297341, 0.298962, 0.294299, 0.295821, 0.300327, 0.294273, 0.300385, 0.300758, 0.297525, 0.298771, 0.300572, 0.296961, 0.297537, 0.302398, 0.297105, 0.299835, 0.302088, 0.29876, 0.300305, 0.304773, 0.307773], 'N': [0, 0, 4.469e-06, 0, 0, 0, 0, 0, 2.36219e-05, 1.18109e-05, 6.06508e-06, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 2.20276e-05, 1.05351e-05, 1.27699e-06, 0, 0, 0, 0, 0, 0, 0, 0, 9.58009e-07, 0, 0, 0, 1.59705e-06, 3.1941e-07, 0, 0, 0, 0.00202378], 'GC': [0.556441, 0.57827, 0.563519, 0.571754, 0.580331, 0.566555, 0.575202, 0.578977, 0.565538, 0.572091, 0.577652, 0.567277, 0.568957, 0.584125, 0.57092, 0.575716, 0.582911, 0.571716, 0.574854, 0.582409, 0.573573, 0.576489, 0.584091, 0.574863, 0.580983, 0.584932, 0.574592, 0.579914, 0.584812, 0.575025, 0.579921, 0.58557, 0.573473, 0.576477, 0.583698, 0.573216, 0.578005, 0.58475, 0.576761, 0.580088, 0.585548, 0.574892, 0.578868, 0.585406, 0.57661, 0.576568, 0.585483, 0.57497, 0.580286, 0.583314, 0.579983, 0.580623, 0.587743, 0.580089, 0.582049, 0.588561, 0.579513, 0.586258, 0.590126, 0.581755, 0.583818, 0.591728, 0.601555]}, 'kmer_count': {'AAAAA': 369795, 'AAAAT': 173488, 'AAAAC': 135439, 'AAAAG': 175022, 'AAATA': 151392, 'AAATT': 115972, 'AAATC': 108565, 'AAATG': 125764, 'AAACA': 165647, 'AAACT': 145070, 'AAACC': 121625, 'AAACG': 46751, 'AAAGA': 156576, 'AAAGT': 139196, 'AAAGC': 155958, 'AAAGG': 173960, 'AATAA': 117998, 'AATAT': 72852, 'AATAC': 63896, 'AATAG': 69635, 'AATTA': 64940, 'AATTT': 122467, 'AATTC': 98388, 'AATTG': 65966, 'AATCA': 88664, 'AATCT': 134983, 'AATCC': 108089, 'AATCG': 39153, 'AATGA': 107963, 'AATGT': 105543, 'AATGC': 105067, 'AATGG': 152041, 'AACAA': 115519, 'AACAT': 109944, 'AACAC': 126593, 'AACAG': 162240, 'AACTA': 40114, 'AACTT': 169030, 'AACTC': 147948, 'AACTG': 156483, 'AACCA': 110493, 'AACCT': 102904, 'AACCC': 116106, 'AACCG': 43482, 'AACGA': 25963, 'AACGT': 34548, 'AACGC': 44436, 'AACGG': 55517, 'AAGAA': 175589, 'AAGAT': 130019, 'AAGAC': 111890, 'AAGAG': 185195, 'AAGTA': 89818, 'AAGTT': 133890, 'AAGTC': 143676, 'AAGTG': 158679, 'AAGCA': 138224, 'AAGCT': 151243, 'AAGCC': 208748, 'AAGCG': 103894, 'AAGGA': 188403, 'AAGGT': 153746, 'AAGGC': 253371, 'AAGGG': 245764, 'ATAAA': 129956, 'ATAAT': 59300, 'ATAAC': 44261, 'ATAAG': 58689, 'ATATA': 59981, 'ATATT': 71128, 'ATATC': 47613, 'ATATG': 48297, 'ATACA': 90249, 'ATACT': 85073, 'ATACC': 49986, 'ATACG': 19393, 'ATAGA': 75310, 'ATAGT': 68393, 'ATAGC': 81711, 'ATAGG': 89179, 'ATTAA': 62328, 'ATTAT': 44076, 'ATTAC': 53298, 'ATTAG': 41811, 'ATTTA': 62853, 'ATTTT': 151753, 'ATTTC': 129786, 'ATTTG': 126746, 'ATTCA': 108201, 'ATTCT': 133966, 'ATTCC': 116148, 'ATTCG': 41163, 'ATTGA': 80733, 'ATTGT': 68504, 'ATTGC': 75091, 'ATTGG': 101187, 'ATCAA': 86285, 'ATCAT': 157323, 'ATCAC': 115909, 'ATCAG': 160773, 'ATCTA': 36808, 'ATCTT': 277999, 'ATCTC': 221971, 'ATCTG': 213692, 'ATCCA': 178003, 'ATCCT': 170020, 'ATCCC': 120825, 'ATCCG': 76032, 'ATCGA': 37403, 'ATCGT': 49996, 'ATCGC': 52523, 'ATCGG': 52429, 'ATGAA': 156591, 'ATGAT': 167176, 'ATGAC': 121528, 'ATGAG': 197183, 'ATGTA': 98005, 'ATGTT': 186011, 'ATGTC': 180723, 'ATGTG': 127755, 'ATGCA': 105674, 'ATGCT': 129700, 'ATGCC': 178873, 'ATGCG': 119597, 'ATGGA': 155448, 'ATGGT': 197604, 'ATGGC': 265667, 'ATGGG': 219793, 'ACAAA': 176999, 'ACAAT': 99810, 'ACAAC': 77837, 'ACAAG': 123646, 'ACATA': 83244, 'ACATT': 121931, 'ACATC': 139573, 'ACATG': 148398, 'ACACA': 192679, 'ACACT': 110290, 'ACACC': 181609, 'ACACG': 107527, 'ACAGA': 186396, 'ACAGT': 135027, 'ACAGC': 271919, 'ACAGG': 280987, 'ACTAA': 38622, 'ACTAT': 36524, 'ACTAC': 33345, 'ACTAG': 37688, 'ACTTA': 38072, 'ACTTT': 139237, 'ACTTC': 201169, 'ACTTG': 218456, 'ACTCA': 137197, 'ACTCT': 127297, 'ACTCC': 190898, 'ACTCG': 125629, 'ACTGA': 125125, 'ACTGT': 141089, 'ACTGC': 201811, 'ACTGG': 240099, 'ACCAA': 90875, 'ACCAT': 136323, 'ACCAC': 240053, 'ACCAG': 297687, 'ACCTA': 31704, 'ACCTT': 173505, 'ACCTC': 227779, 'ACCTG': 227208, 'ACCCA': 178955, 'ACCCT': 150940, 'ACCCC': 173868, 'ACCCG': 101092, 'ACCGA': 61367, 'ACCGT': 65556, 'ACCGC': 86174, 'ACCGG': 78599, 'ACGAA': 58269, 'ACGAT': 64783, 'ACGAC': 57409, 'ACGAG': 78590, 'ACGTA': 37407, 'ACGTT': 87804, 'ACGTC': 102378, 'ACGTG': 95437, 'ACGCA': 79093, 'ACGCT': 81082, 'ACGCC': 127390, 'ACGCG': 61753, 'ACGGA': 85537, 'ACGGT': 94390, 'ACGGC': 131874, 'ACGGG': 132762, 'AGAAA': 197626, 'AGAAT': 101131, 'AGAAC': 151016, 'AGAAG': 286779, 'AGATA': 84679, 'AGATT': 79964, 'AGATC': 123570, 'AGATG': 235341, 'AGACA': 151230, 'AGACT': 99704, 'AGACC': 169521, 'AGACG': 107165, 'AGAGA': 211209, 'AGAGT': 116907, 'AGAGC': 206054, 'AGAGG': 338741, 'AGTAA': 74143, 'AGTAT': 57023, 'AGTAC': 74286, 'AGTAG': 108301, 'AGTTA': 56516, 'AGTTT': 137698, 'AGTTC': 146047, 'AGTTG': 146692, 'AGTCA': 132846, 'AGTCT': 146812, 'AGTCC': 208901, 'AGTCG': 91962, 'AGTGA': 156705, 'AGTGT': 120205, 'AGTGC': 158158, 'AGTGG': 244127, 'AGCAA': 123107, 'AGCAT': 143377, 'AGCAC': 234925, 'AGCAG': 413206, 'AGCTA': 61158, 'AGCTT': 243922, 'AGCTC': 317769, 'AGCTG': 413709, 'AGCCA': 301895, 'AGCCT': 245364, 'AGCCC': 326526, 'AGCCG': 209334, 'AGCGA': 86657, 'AGCGT': 126225, 'AGCGC': 178709, 'AGCGG': 212775, 'AGGAA': 304068, 'AGGAT': 200061, 'AGGAC': 227791, 'AGGAG': 409742, 'AGGTA': 139068, 'AGGTT': 159307, 'AGGTC': 258189, 'AGGTG': 359106, 'AGGCA': 313756, 'AGGCT': 326592, 'AGGCC': 403411, 'AGGCG': 254691, 'AGGGA': 286577, 'AGGGT': 243528, 'AGGGC': 438732, 'AGGGG': 487953, 'TAAAA': 148878, 'TAAAT': 87770, 'TAAAC': 60564, 'TAAAG': 69399, 'TAATA': 48357, 'TAATT': 67043, 'TAATC': 61960, 'TAATG': 66315, 'TAACA': 67150, 'TAACT': 63814, 'TAACC': 40349, 'TAACG': 11592, 'TAAGA': 61919, 'TAAGT': 44203, 'TAAGC': 51456, 'TAAGG': 76327, 'TATAA': 48021, 'TATAT': 48900, 'TATAC': 31070, 'TATAG': 42556, 'TATTA': 35236, 'TATTT': 122173, 'TATTC': 66232, 'TATTG': 67782, 'TATCA': 57123, 'TATCT': 66147, 'TATCC': 57879, 'TATCG': 23984, 'TATGA': 47861, 'TATGT': 48917, 'TATGC': 34905, 'TATGG': 72169, 'TACAA': 80850, 'TACAT': 75910, 'TACAC': 80362, 'TACAG': 119521, 'TACTA': 22247, 'TACTT': 121448, 'TACTC': 90233, 'TACTG': 95342, 'TACCA': 65976, 'TACCT': 54961, 'TACCC': 60808, 'TACCG': 30938, 'TACGA': 19575, 'TACGT': 27703, 'TACGC': 20976, 'TACGG': 42970, 'TAGAA': 96000, 'TAGAT': 77885, 'TAGAC': 61666, 'TAGAG': 103941, 'TAGTA': 59926, 'TAGTT': 69047, 'TAGTC': 77461, 'TAGTG': 84078, 'TAGCA': 73053, 'TAGCT': 86017, 'TAGCC': 112973, 'TAGCG': 66048, 'TAGGA': 108346, 'TAGGT': 98276, 'TAGGC': 110843, 'TAGGG': 128738, 'TTAAA': 116075, 'TTAAT': 78175, 'TTAAC': 50975, 'TTAAG': 65418, 'TTATA': 47876, 'TTATT': 86242, 'TTATC': 56952, 'TTATG': 51122, 'TTACA': 83618, 'TTACT': 48520, 'TTACC': 40786, 'TTACG': 27228, 'TTAGA': 39537, 'TTAGT': 54636, 'TTAGC': 51260, 'TTAGG': 73859, 'TTTAA': 115187, 'TTTAT': 101531, 'TTTAC': 64072, 'TTTAG': 63782, 'TTTTA': 117740, 'TTTTT': 486621, 'TTTTC': 218201, 'TTTTG': 167875, 'TTTCA': 160633, 'TTTCT': 313291, 'TTTCC': 203246, 'TTTCG': 39754, 'TTTGA': 132504, 'TTTGT': 191566, 'TTTGC': 153996, 'TTTGG': 184837, 'TTCAA': 144822, 'TTCAT': 198898, 'TTCAC': 230900, 'TTCAG': 278158, 'TTCTA': 71625, 'TTCTT': 467219, 'TTCTC': 412683, 'TTCTG': 314567, 'TTCCA': 215494, 'TTCCT': 286826, 'TTCCC': 226520, 'TTCCG': 141527, 'TTCGA': 49385, 'TTCGT': 40310, 'TTCGC': 43664, 'TTCGG': 70909, 'TTGAA': 182323, 'TTGAT': 197860, 'TTGAC': 146295, 'TTGAG': 222798, 'TTGTA': 151408, 'TTGTT': 204381, 'TTGTC': 234847, 'TTGTG': 163334, 'TTGCA': 143602, 'TTGCT': 194011, 'TTGCC': 203234, 'TTGCG': 134391, 'TTGGA': 209420, 'TTGGT': 231195, 'TTGGC': 295300, 'TTGGG': 285690, 'TCAAA': 171374, 'TCAAT': 136858, 'TCAAC': 77117, 'TCAAG': 117139, 'TCATA': 101736, 'TCATT': 119658, 'TCATC': 309123, 'TCATG': 213277, 'TCACA': 206932, 'TCACT': 184138, 'TCACC': 229301, 'TCACG': 112015, 'TCAGA': 166486, 'TCAGT': 158235, 'TCAGC': 337104, 'TCAGG': 339408, 'TCTAA': 47006, 'TCTAT': 55210, 'TCTAC': 65983, 'TCTAG': 64617, 'TCTTA': 77779, 'TCTTT': 249060, 'TCTTC': 500380, 'TCTTG': 463318, 'TCTCA': 181389, 'TCTCT': 275158, 'TCTCC': 469936, 'TCTCG': 207012, 'TCTGA': 178643, 'TCTGT': 213539, 'TCTGC': 328790, 'TCTGG': 432754, 'TCCAA': 146595, 'TCCAT': 199416, 'TCCAC': 360082, 'TCCAG': 553577, 'TCCTA': 61406, 'TCCTT': 358761, 'TCCTC': 617096, 'TCCTG': 378654, 'TCCCA': 269847, 'TCCCT': 190186, 'TCCCC': 288569, 'TCCCG': 233558, 'TCCGA': 89576, 'TCCGT': 128605, 'TCCGC': 176231, 'TCCGG': 212123, 'TCGAA': 106113, 'TCGAT': 101361, 'TCGAC': 42469, 'TCGAG': 65419, 'TCGTA': 85163, 'TCGTT': 76368, 'TCGTC': 158438, 'TCGTG': 105215, 'TCGCA': 84423, 'TCGCT': 110247, 'TCGCC': 134298, 'TCGCG': 75940, 'TCGGA': 93248, 'TCGGT': 92391, 'TCGGC': 190534, 'TCGGG': 206323, 'TGAAA': 123491, 'TGAAT': 100513, 'TGAAC': 144697, 'TGAAG': 306950, 'TGATA': 62082, 'TGATT': 69634, 'TGATC': 162540, 'TGATG': 316751, 'TGACA': 153067, 'TGACT': 114628, 'TGACC': 185550, 'TGACG': 138429, 'TGAGA': 185700, 'TGAGT': 130786, 'TGAGC': 275781, 'TGAGG': 368452, 'TGTAA': 82560, 'TGTAT': 71350, 'TGTAC': 110088, 'TGTAG': 201091, 'TGTTA': 69675, 'TGTTT': 166177, 'TGTTC': 195307, 'TGTTG': 274175, 'TGTCA': 191231, 'TGTCT': 216555, 'TGTCC': 324966, 'TGTCG': 131721, 'TGTGA': 139674, 'TGTGT': 176158, 'TGTGC': 185982, 'TGTGG': 302099, 'TGCAA': 114339, 'TGCAT': 142849, 'TGCAC': 196409, 'TGCAG': 377110, 'TGCTA': 53249, 'TGCTT': 226092, 'TGCTC': 294658, 'TGCTG': 468256, 'TGCCA': 254932, 'TGCCT': 235643, 'TGCCC': 319196, 'TGCCG': 226287, 'TGCGA': 54184, 'TGCGT': 90697, 'TGCGC': 161690, 'TGCGG': 251390, 'TGGAA': 242543, 'TGGAT': 177410, 'TGGAC': 202541, 'TGGAG': 411959, 'TGGTA': 125895, 'TGGTT': 182663, 'TGGTC': 321165, 'TGGTG': 396371, 'TGGCA': 314343, 'TGGCT': 337964, 'TGGCC': 487607, 'TGGCG': 254985, 'TGGGA': 297516, 'TGGGT': 291191, 'TGGGC': 439534, 'TGGGG': 622897, 'CAAAA': 183006, 'CAAAT': 110572, 'CAAAC': 168216, 'CAAAG': 218552, 'CAATA': 61559, 'CAATT': 76475, 'CAATC': 98214, 'CAATG': 161816, 'CAACA': 127773, 'CAACT': 94573, 'CAACC': 94126, 'CAACG': 49059, 'CAAGA': 118210, 'CAAGT': 120397, 'CAAGC': 115948, 'CAAGG': 203434, 'CATAA': 69881, 'CATAT': 54310, 'CATAC': 97210, 'CATAG': 138734, 'CATTA': 63954, 'CATTT': 126424, 'CATTC': 150696, 'CATTG': 128932, 'CATCA': 231976, 'CATCT': 290000, 'CATCC': 249039, 'CATCG': 91684, 'CATGA': 206983, 'CATGT': 184853, 'CATGC': 177254, 'CATGG': 277933, 'CACAA': 178184, 'CACAT': 183649, 'CACAC': 233007, 'CACAG': 356344, 'CACTA': 50728, 'CACTT': 161672, 'CACTC': 187020, 'CACTG': 317813, 'CACCA': 381217, 'CACCT': 327625, 'CACCC': 247279, 'CACCG': 132542, 'CACGA': 124415, 'CACGT': 163200, 'CACGC': 175385, 'CACGG': 208303, 'CAGAA': 234597, 'CAGAT': 171772, 'CAGAC': 183833, 'CAGAG': 323628, 'CAGTA': 98247, 'CAGTT': 161896, 'CAGTC': 184720, 'CAGTG': 264965, 'CAGCA': 480790, 'CAGCT': 519399, 'CAGCC': 476341, 'CAGCG': 280012, 'CAGGA': 475410, 'CAGGT': 395490, 'CAGGC': 527098, 'CAGGG': 632139, 'CTAAA': 51931, 'CTAAT': 33923, 'CTAAC': 40079, 'CTAAG': 52422, 'CTATA': 24269, 'CTATT': 54030, 'CTATC': 46104, 'CTATG': 47722, 'CTACA': 61933, 'CTACT': 52849, 'CTACC': 51794, 'CTACG': 27418, 'CTAGA': 66074, 'CTAGT': 25892, 'CTAGC': 50301, 'CTAGG': 81921, 'CTTAA': 73079, 'CTTAT': 46850, 'CTTAC': 36180, 'CTTAG': 69415, 'CTTTA': 110948, 'CTTTT': 192679, 'CTTTC': 195534, 'CTTTG': 220703, 'CTTCA': 397800, 'CTTCT': 557823, 'CTTCC': 389069, 'CTTCG': 76856, 'CTTGA': 331172, 'CTTGT': 299834, 'CTTGC': 304769, 'CTTGG': 471530, 'CTCAA': 151168, 'CTCAT': 226372, 'CTCAC': 177776, 'CTCAG': 335529, 'CTCTA': 73236, 'CTCTT': 298449, 'CTCTC': 253455, 'CTCTG': 374569, 'CTCCA': 545836, 'CTCCT': 617404, 'CTCCC': 338793, 'CTCCG': 248804, 'CTCGA': 138266, 'CTCGT': 208577, 'CTCGC': 206048, 'CTCGG': 290040, 'CTGAA': 169413, 'CTGAT': 110742, 'CTGAC': 163368, 'CTGAG': 299060, 'CTGTA': 121008, 'CTGTT': 173463, 'CTGTC': 265902, 'CTGTG': 306731, 'CTGCA': 386773, 'CTGCT': 461603, 'CTGCC': 425594, 'CTGCG': 183925, 'CTGGA': 380813, 'CTGGT': 355552, 'CTGGC': 449088, 'CTGGG': 684454, 'CCAAA': 189461, 'CCAAT': 74612, 'CCAAC': 119578, 'CCAAG': 184575, 'CCATA': 94495, 'CCATT': 131110, 'CCATC': 229540, 'CCATG': 294915, 'CCACA': 318343, 'CCACT': 226035, 'CCACC': 441795, 'CCACG': 283025, 'CCAGA': 291955, 'CCAGT': 221526, 'CCAGC': 599842, 'CCAGG': 761902, 'CCTAA': 41887, 'CCTAT': 47712, 'CCTAC': 39942, 'CCTAG': 59370, 'CCTTA': 51995, 'CCTTT': 163415, 'CCTTC': 371796, 'CCTTG': 412980, 'CCTCA': 308470, 'CCTCT': 333849, 'CCTCC': 629705, 'CCTCG': 305153, 'CCTGA': 196695, 'CCTGT': 207437, 'CCTGC': 386459, 'CCTGG': 552988, 'CCCAA': 166672, 'CCCAT': 198076, 'CCCAC': 314493, 'CCCAG': 535240, 'CCCTA': 50505, 'CCCTT': 216105, 'CCCTC': 315625, 'CCCTG': 337084, 'CCCCA': 365866, 'CCCCT': 245547, 'CCCCC': 312603, 'CCCCG': 272300, 'CCCGA': 137864, 'CCCGT': 146725, 'CCCGC': 251811, 'CCCGG': 353358, 'CCGAA': 82591, 'CCGAT': 74415, 'CCGAC': 86857, 'CCGAG': 175594, 'CCGTA': 73596, 'CCGTT': 99046, 'CCGTC': 174007, 'CCGTG': 185357, 'CCGCA': 172842, 'CCGCT': 233000, 'CCGCC': 314761, 'CCGCG': 163208, 'CCGGA': 161303, 'CCGGT': 158241, 'CCGGC': 302083, 'CCGGG': 359137, 'CGAAA': 44263, 'CGAAT': 42065, 'CGAAC': 64897, 'CGAAG': 151863, 'CGATA': 20796, 'CGATT': 30737, 'CGATC': 85322, 'CGATG': 186683, 'CGACA': 60640, 'CGACT': 66509, 'CGACC': 79332, 'CGACG': 52053, 'CGAGA': 86385, 'CGAGT': 69170, 'CGAGC': 106281, 'CGAGG': 189788, 'CGTAA': 21026, 'CGTAT': 26577, 'CGTAC': 55709, 'CGTAG': 141631, 'CGTTA': 18436, 'CGTTT': 73375, 'CGTTC': 119914, 'CGTTG': 137086, 'CGTCA': 106905, 'CGTCT': 150867, 'CGTCC': 200946, 'CGTCG': 130847, 'CGTGA': 95665, 'CGTGT': 105024, 'CGTGC': 143672, 'CGTGG': 228215, 'CGCAA': 41580, 'CGCAT': 67584, 'CGCAC': 127494, 'CGCAG': 270876, 'CGCTA': 20002, 'CGCTT': 128555, 'CGCTC': 222456, 'CGCTG': 249627, 'CGCCA': 178940, 'CGCCT': 175768, 'CGCCC': 252489, 'CGCCG': 214486, 'CGCGA': 57518, 'CGCGT': 67256, 'CGCGC': 132378, 'CGCGG': 195816, 'CGGAA': 104933, 'CGGAT': 125150, 'CGGAC': 105306, 'CGGAG': 187795, 'CGGTA': 91495, 'CGGTT': 84424, 'CGGTC': 140140, 'CGGTG': 212839, 'CGGCA': 183173, 'CGGCT': 211638, 'CGGCC': 361551, 'CGGCG': 246235, 'CGGGA': 189421, 'CGGGT': 135892, 'CGGGC': 341109, 'CGGGG': 371854, 'GAAAA': 151628, 'GAAAT': 126502, 'GAAAC': 113630, 'GAAAG': 162854, 'GAATA': 62015, 'GAATT': 91768, 'GAATC': 100593, 'GAATG': 113136, 'GAACA': 152940, 'GAACT': 209275, 'GAACC': 118551, 'GAACG': 53229, 'GAAGA': 266212, 'GAAGT': 222880, 'GAAGC': 278547, 'GAAGG': 389474, 'GATAA': 56015, 'GATAT': 51201, 'GATAC': 52542, 'GATAG': 64236, 'GATTA': 35717, 'GATTT': 99094, 'GATTC': 83094, 'GATTG': 61914, 'GATCA': 141176, 'GATCT': 257875, 'GATCC': 131963, 'GATCG': 38323, 'GATGA': 278171, 'GATGT': 251996, 'GATGC': 215846, 'GATGG': 337253, 'GACAA': 103341, 'GACAT': 122430, 'GACAC': 151929, 'GACAG': 236168, 'GACTA': 32673, 'GACTT': 145614, 'GACTC': 157126, 'GACTG': 138264, 'GACCA': 208767, 'GACCT': 175015, 'GACCC': 185607, 'GACCG': 85220, 'GACGA': 89102, 'GACGT': 97493, 'GACGC': 109721, 'GACGG': 138372, 'GAGAA': 230286, 'GAGAT': 144540, 'GAGAC': 172027, 'GAGAG': 260400, 'GAGTA': 65870, 'GAGTT': 121770, 'GAGTC': 174864, 'GAGTG': 171150, 'GAGCA': 222894, 'GAGCT': 281489, 'GAGCC': 288818, 'GAGCG': 155563, 'GAGGA': 365395, 'GAGGT': 264627, 'GAGGC': 411357, 'GAGGG': 453386, 'GTAAA': 67478, 'GTAAT': 70863, 'GTAAC': 46578, 'GTAAG': 56660, 'GTATA': 38133, 'GTATT': 77403, 'GTATC': 53014, 'GTATG': 56168, 'GTACA': 120563, 'GTACT': 143930, 'GTACC': 71161, 'GTACG': 37521, 'GTAGA': 158257, 'GTAGT': 141285, 'GTAGC': 154284, 'GTAGG': 200603, 'GTTAA': 58523, 'GTTAT': 48105, 'GTTAC': 46214, 'GTTAG': 42557, 'GTTTA': 50159, 'GTTTT': 155047, 'GTTTC': 171331, 'GTTTG': 144867, 'GTTCA': 184890, 'GTTCT': 257278, 'GTTCC': 163275, 'GTTCG': 46965, 'GTTGA': 201824, 'GTTGT': 192264, 'GTTGC': 141480, 'GTTGG': 264388, 'GTCAA': 118806, 'GTCAT': 158798, 'GTCAC': 206804, 'GTCAG': 224014, 'GTCTA': 50226, 'GTCTT': 241815, 'GTCTC': 242802, 'GTCTG': 247371, 'GTCCA': 316006, 'GTCCT': 338265, 'GTCCC': 300127, 'GTCCG': 142293, 'GTCGA': 90825, 'GTCGT': 126807, 'GTCGC': 103498, 'GTCGG': 169170, 'GTGAA': 165671, 'GTGAT': 132563, 'GTGAC': 159283, 'GTGAG': 239171, 'GTGTA': 93459, 'GTGTT': 140299, 'GTGTC': 181084, 'GTGTG': 201104, 'GTGCA': 193392, 'GTGCT': 256160, 'GTGCC': 229613, 'GTGCG': 119000, 'GTGGA': 283768, 'GTGGT': 237885, 'GTGGC': 384147, 'GTGGG': 461402, 'GCAAA': 143617, 'GCAAT': 86544, 'GCAAC': 91783, 'GCAAG': 132591, 'GCATA': 81125, 'GCATT': 96191, 'GCATC': 184825, 'GCATG': 190830, 'GCACA': 233880, 'GCACT': 195704, 'GCACC': 240820, 'GCACG': 170004, 'GCAGA': 269608, 'GCAGT': 195376, 'GCAGC': 555375, 'GCAGG': 651734, 'GCTAA': 50441, 'GCTAT': 32383, 'GCTAC': 56488, 'GCTAG': 62373, 'GCTTA': 57790, 'GCTTT': 168568, 'GCTTC': 347960, 'GCTTG': 308227, 'GCTCA': 264869, 'GCTCT': 265062, 'GCTCC': 467787, 'GCTCG': 207198, 'GCTGA': 242185, 'GCTGT': 304543, 'GCTGC': 543052, 'GCTGG': 647462, 'GCCAA': 164668, 'GCCAT': 216422, 'GCCAC': 354238, 'GCCAG': 487290, 'GCCTA': 45221, 'GCCTT': 249773, 'GCCTC': 416803, 'GCCTG': 399798, 'GCCCA': 401203, 'GCCCT': 332431, 'GCCCC': 431919, 'GCCCG': 285238, 'GCCGA': 131311, 'GCCGT': 191342, 'GCCGC': 371931, 'GCCGG': 339292, 'GCGAA': 57360, 'GCGAT': 82450, 'GCGAC': 71686, 'GCGAG': 133727, 'GCGTA': 48786, 'GCGTT': 84964, 'GCGTC': 155768, 'GCGTG': 186642, 'GCGCA': 172178, 'GCGCT': 196095, 'GCGCC': 250711, 'GCGCG': 153533, 'GCGGA': 182803, 'GCGGT': 182912, 'GCGGC': 381868, 'GCGGG': 344098, 'GGAAA': 189209, 'GGAAT': 123871, 'GGAAC': 173543, 'GGAAG': 411251, 'GGATA': 55813, 'GGATT': 98108, 'GGATC': 199289, 'GGATG': 342112, 'GGACA': 248429, 'GGACT': 193336, 'GGACC': 222913, 'GGACG': 137122, 'GGAGA': 324650, 'GGAGT': 216445, 'GGAGC': 361621, 'GGAGG': 599384, 'GGTAA': 63391, 'GGTAT': 69920, 'GGTAC': 134432, 'GGTAG': 203939, 'GGTTA': 49965, 'GGTTT': 143670, 'GGTTC': 190399, 'GGTTG': 241905, 'GGTCA': 275981, 'GGTCT': 268226, 'GGTCC': 362324, 'GGTCG': 135779, 'GGTGA': 303218, 'GGTGT': 213410, 'GGTGC': 308923, 'GGTGG': 592914, 'GGCAA': 175630, 'GGCAT': 198553, 'GGCAC': 284208, 'GGCAG': 612078, 'GGCTA': 67891, 'GGCTT': 285191, 'GGCTC': 371899, 'GGCTG': 604265, 'GGCCA': 490825, 'GGCCT': 455678, 'GGCCC': 557493, 'GGCCG': 387459, 'GGCGA': 146267, 'GGCGT': 192905, 'GGCGC': 301366, 'GGCGG': 434939, 'GGGAA': 244257, 'GGGAT': 189399, 'GGGAC': 267064, 'GGGAG': 492663, 'GGGTA': 114703, 'GGGTT': 197798, 'GGGTC': 325736, 'GGGTG': 443371, 'GGGCA': 458274, 'GGGCT': 450547, 'GGGCC': 646086, 'GGGCG': 322301, 'GGGGA': 414202, 'GGGGT': 406441, 'GGGGC': 657974, 'GGGGG': 620466}, 'overrepresented_sequences': {}} {'total_reads': 3063108, 'total_bases': 177478915, 'q20_bases': 169330633, 'q30_bases': 161384759, 'total_cycles': 58, 'quality_curves': {'A': [36.3823, 36.3622, 36.3904, 36.4026, 36.4078, 36.3971, 36.3933, 36.3979, 36.4072, 36.3722, 36.3763, 36.3376, 36.3521, 36.2909, 36.3069, 36.3012, 36.2921, 36.2325, 36.2224, 36.1823, 36.1888, 36.1465, 36.1128, 36.0667, 36.0739, 36.0667, 36.0049, 35.9956, 35.9277, 35.9213, 35.8697, 35.8149, 35.734, 35.6852, 35.7194, 35.67, 35.6092, 35.5449, 35.5027, 35.4691, 35.3626, 35.326, 35.2544, 35.197, 35.0713, 34.9672, 34.9114, 34.8629, 34.7967, 34.7078, 34.5112, 34.2974, 34.2242, 34.0193, 33.8888, 33.79, 33.5205, 33.3654], 'T': [36.8008, 36.8497, 36.8256, 36.8003, 36.789, 36.7766, 36.7526, 36.7363, 36.7036, 36.6813, 36.6767, 36.6106, 36.6161, 36.604, 36.5826, 36.5747, 36.53, 36.4825, 36.4978, 36.4157, 36.4083, 36.3715, 36.3261, 36.271, 36.256, 36.2664, 36.2146, 36.1599, 36.085, 36.1039, 36.0427, 36.008, 35.9355, 35.8752, 35.852, 35.834, 35.7377, 35.6894, 35.635, 35.59, 35.4608, 35.4478, 35.342, 35.3222, 35.2123, 35.0833, 34.9974, 34.9699, 34.8478, 34.7988, 34.6478, 34.4064, 34.3461, 34.1799, 33.9629, 33.9714, 33.6517, 33.5695], 'C': [36.7295, 36.7545, 36.7717, 36.7674, 36.7553, 36.7655, 36.7565, 36.7667, 36.7636, 36.7267, 36.7298, 36.736, 36.7123, 36.7204, 36.7225, 36.7013, 36.6944, 36.6722, 36.6495, 36.6307, 36.6272, 36.6081, 36.5653, 36.5788, 36.586, 36.5548, 36.5432, 36.4917, 36.4684, 36.4632, 36.4476, 36.3935, 36.3609, 36.3263, 36.3204, 36.3009, 36.2533, 36.1797, 36.1775, 36.1043, 36.0287, 35.9885, 35.931, 35.8581, 35.7979, 35.6928, 35.5941, 35.5713, 35.4632, 35.3662, 35.2897, 35.0766, 34.9611, 34.8246, 34.6112, 34.5125, 34.3208, 34.0835], 'G': [36.3353, 36.3693, 36.3877, 36.3867, 36.3814, 36.3887, 36.3902, 36.3631, 36.3621, 36.3425, 36.3202, 36.3096, 36.31, 36.274, 36.2761, 36.2667, 36.2415, 36.2134, 36.2003, 36.1407, 36.1684, 36.1275, 36.072, 36.0503, 36.0642, 36.0512, 36.0387, 35.9849, 35.9219, 35.9246, 35.894, 35.831, 35.8079, 35.7251, 35.7434, 35.7079, 35.6503, 35.5902, 35.5494, 35.5037, 35.3665, 35.371, 35.2827, 35.2105, 35.1277, 35.0178, 34.938, 34.9074, 34.8031, 34.7161, 34.5538, 34.3461, 34.2108, 34.0581, 33.8762, 33.7489, 33.5415, 33.3548], 'mean': [36.5582, 36.5769, 36.5886, 36.5804, 36.5758, 36.5769, 36.5674, 36.5642, 36.5582, 36.5279, 36.5221, 36.4988, 36.4971, 36.4741, 36.4757, 36.4631, 36.443, 36.4055, 36.3964, 36.3468, 36.3547, 36.3195, 36.2768, 36.2562, 36.259, 36.2468, 36.2173, 36.172, 36.1168, 36.1221, 36.0813, 36.0272, 35.9824, 35.9166, 35.9298, 35.902, 35.8398, 35.7753, 35.7449, 35.6917, 35.5812, 35.5633, 35.481, 35.4213, 35.3298, 35.2221, 35.1391, 35.1096, 35.0085, 34.9233, 34.7862, 34.5642, 34.4644, 34.3076, 34.1153, 34.0298, 33.7955, 33.6172]}, 'content_curves': {'A': [0.21196, 0.216963, 0.214099, 0.218864, 0.226016, 0.214564, 0.217436, 0.219842, 0.217267, 0.21888, 0.224678, 0.219664, 0.218752, 0.222589, 0.214001, 0.216915, 0.221248, 0.217429, 0.218875, 0.221326, 0.218382, 0.218105, 0.221317, 0.214788, 0.219832, 0.220182, 0.215391, 0.21886, 0.222118, 0.217046, 0.221105, 0.222845, 0.217304, 0.219459, 0.222573, 0.216041, 0.216939, 0.223334, 0.219441, 0.220401, 0.225294, 0.216249, 0.219313, 0.220399, 0.218855, 0.218354, 0.223881, 0.217789, 0.219888, 0.221288, 0.214866, 0.216148, 0.219732, 0.21435, 0.21911, 0.222783, 0.215152, 0.219578], 'T': [0.205691, 0.197209, 0.197351, 0.190249, 0.193138, 0.191704, 0.189735, 0.198043, 0.195478, 0.19261, 0.199799, 0.194835, 0.193093, 0.196639, 0.196542, 0.194158, 0.195512, 0.198672, 0.19401, 0.197384, 0.192801, 0.190097, 0.196539, 0.19122, 0.193644, 0.196595, 0.194695, 0.193768, 0.197199, 0.192562, 0.190791, 0.196278, 0.194502, 0.193543, 0.196483, 0.195467, 0.190825, 0.194693, 0.193155, 0.190851, 0.19232, 0.192708, 0.192666, 0.198209, 0.192108, 0.19169, 0.195243, 0.192572, 0.191233, 0.197368, 0.194771, 0.190532, 0.196334, 0.195204, 0.191708, 0.195392, 0.194244, 0.192393], 'C': [0.297219, 0.297152, 0.296654, 0.292941, 0.293389, 0.297225, 0.295638, 0.296132, 0.297796, 0.295918, 0.291985, 0.295606, 0.296217, 0.29489, 0.297507, 0.297179, 0.296172, 0.298908, 0.297388, 0.297065, 0.295613, 0.296425, 0.295604, 0.303122, 0.297975, 0.297773, 0.300521, 0.297681, 0.295371, 0.304568, 0.298425, 0.293452, 0.29959, 0.296812, 0.295348, 0.301048, 0.301404, 0.29846, 0.301675, 0.299852, 0.298246, 0.303292, 0.298561, 0.29945, 0.300297, 0.300558, 0.297948, 0.300965, 0.300407, 0.296401, 0.303281, 0.300566, 0.299028, 0.305326, 0.299696, 0.298995, 0.304323, 0.30029], 'G': [0.28513, 0.288674, 0.291896, 0.297946, 0.287458, 0.296507, 0.297175, 0.285983, 0.289459, 0.292592, 0.283493, 0.289846, 0.291926, 0.285875, 0.291951, 0.291748, 0.287063, 0.284915, 0.289726, 0.284138, 0.293203, 0.295345, 0.286541, 0.290871, 0.288548, 0.285446, 0.289393, 0.28969, 0.285312, 0.285814, 0.289652, 0.287426, 0.288604, 0.289974, 0.285597, 0.287418, 0.290831, 0.283513, 0.285722, 0.288868, 0.284139, 0.287751, 0.289446, 0.281872, 0.288648, 0.289394, 0.282927, 0.288674, 0.288473, 0.284943, 0.287083, 0.292677, 0.284898, 0.28512, 0.289467, 0.28283, 0.286281, 0.287738], 'N': [0, 1.95879e-06, 0, 0, 0, 0, 1.59968e-05, 0, 0, 0, 4.43993e-05, 4.79905e-05, 1.14263e-05, 6.52932e-06, 0, 0, 5.54992e-06, 7.67195e-05, 0, 8.74928e-05, 0, 2.84025e-05, 0, 0, 0, 3.59113e-06, 0, 9.79409e-07, 0, 9.79417e-06, 2.84033e-05, 0, 0, 0.000213217, 0, 2.48181e-05, 0, 0, 6.85898e-06, 2.77644e-05, 0, 0, 1.40489e-05, 6.99253e-05, 9.21552e-05, 3.5952e-06, 0, 0, 3.27054e-07, 0, 0, 7.63031e-05, 8.52032e-06, 0, 1.8069e-05, 0, 0, 0], 'GC': [0.582349, 0.585827, 0.58855, 0.590887, 0.580846, 0.593732, 0.592813, 0.582115, 0.587255, 0.58851, 0.575478, 0.585452, 0.588143, 0.580765, 0.589458, 0.588927, 0.583235, 0.583823, 0.587115, 0.581203, 0.588816, 0.59177, 0.582144, 0.593992, 0.586524, 0.583219, 0.589914, 0.587371, 0.580683, 0.590382, 0.588076, 0.580878, 0.588194, 0.586785, 0.580945, 0.588467, 0.592236, 0.581973, 0.587397, 0.58872, 0.582386, 0.591043, 0.588007, 0.581322, 0.588945, 0.589953, 0.580875, 0.589638, 0.588879, 0.581344, 0.590364, 0.593243, 0.583926, 0.590446, 0.589163, 0.581825, 0.590604, 0.588029]}, 'kmer_count': {'AAAAA': 277244, 'AAAAT': 117082, 'AAAAC': 142281, 'AAAAG': 163001, 'AAATA': 90839, 'AAATT': 92355, 'AAATC': 81210, 'AAATG': 108224, 'AAACA': 157073, 'AAACT': 111605, 'AAACC': 129853, 'AAACG': 63055, 'AAAGA': 220725, 'AAAGT': 120655, 'AAAGC': 151069, 'AAAGG': 146373, 'AATAA': 59376, 'AATAT': 56508, 'AATAC': 59798, 'AATAG': 39090, 'AATTA': 51424, 'AATTT': 93851, 'AATTC': 71659, 'AATTG': 58970, 'AATCA': 59600, 'AATCT': 73257, 'AATCC': 91845, 'AATCG': 23944, 'AATGA': 95880, 'AATGT': 101842, 'AATGC': 84638, 'AATGG': 119976, 'AACAA': 189683, 'AACAT': 156646, 'AACAC': 110486, 'AACAG': 159318, 'AACTA': 55963, 'AACTT': 118899, 'AACTC': 105125, 'AACTG': 141909, 'AACCA': 158770, 'AACCT': 144242, 'AACCC': 168815, 'AACCG': 71334, 'AACGA': 67195, 'AACGT': 71427, 'AACGC': 75086, 'AACGG': 87058, 'AAGAA': 414236, 'AAGAT': 232242, 'AAGAC': 211105, 'AAGAG': 267033, 'AAGTA': 99142, 'AAGTT': 143725, 'AAGTC': 138807, 'AAGTG': 141491, 'AAGCA': 196006, 'AAGCT': 222268, 'AAGCC': 247803, 'AAGCG': 126083, 'AAGGA': 313166, 'AAGGT': 172877, 'AAGGC': 234997, 'AAGGG': 193951, 'ATAAA': 74671, 'ATAAT': 34779, 'ATAAC': 39876, 'ATAAG': 39054, 'ATATA': 37788, 'ATATT': 54431, 'ATATC': 48181, 'ATATG': 45863, 'ATACA': 61214, 'ATACT': 46412, 'ATACC': 50375, 'ATACG': 22757, 'ATAGA': 40935, 'ATAGT': 32196, 'ATAGC': 31142, 'ATAGG': 36209, 'ATTAA': 58451, 'ATTAT': 43855, 'ATTAC': 59995, 'ATTAG': 24717, 'ATTTA': 61101, 'ATTTT': 136197, 'ATTTC': 105393, 'ATTTG': 86043, 'ATTCA': 88913, 'ATTCT': 91190, 'ATTCC': 92711, 'ATTCG': 42914, 'ATTGA': 111216, 'ATTGT': 80380, 'ATTGC': 75616, 'ATTGG': 64183, 'ATCAA': 168351, 'ATCAT': 144232, 'ATCAC': 111845, 'ATCAG': 101340, 'ATCTA': 65293, 'ATCTT': 132835, 'ATCTC': 131204, 'ATCTG': 149820, 'ATCCA': 161347, 'ATCCT': 179968, 'ATCCC': 157917, 'ATCCG': 119936, 'ATCGA': 95600, 'ATCGT': 59300, 'ATCGC': 67947, 'ATCGG': 61702, 'ATGAA': 179976, 'ATGAT': 145899, 'ATGAC': 135235, 'ATGAG': 201477, 'ATGTA': 60773, 'ATGTT': 90618, 'ATGTC': 123515, 'ATGTG': 164964, 'ATGCA': 122202, 'ATGCT': 125278, 'ATGCC': 182572, 'ATGCG': 60159, 'ATGGA': 192382, 'ATGGT': 134435, 'ATGGC': 214364, 'ATGGG': 174276, 'ACAAA': 160479, 'ACAAT': 64389, 'ACAAC': 160007, 'ACAAG': 288805, 'ACATA': 38519, 'ACATT': 92517, 'ACATC': 214967, 'ACATG': 168954, 'ACACA': 149112, 'ACACT': 92776, 'ACACC': 171049, 'ACACG': 87854, 'ACAGA': 189913, 'ACAGT': 118476, 'ACAGC': 260520, 'ACAGG': 183377, 'ACTAA': 43085, 'ACTAT': 55607, 'ACTAC': 118973, 'ACTAG': 21905, 'ACTTA': 40770, 'ACTTT': 118328, 'ACTTC': 188530, 'ACTTG': 89695, 'ACTCA': 106916, 'ACTCT': 104985, 'ACTCC': 181401, 'ACTCG': 61096, 'ACTGA': 135258, 'ACTGT': 121732, 'ACTGC': 181484, 'ACTGG': 199455, 'ACCAA': 205393, 'ACCAT': 190067, 'ACCAC': 191594, 'ACCAG': 317005, 'ACCTA': 85218, 'ACCTT': 132756, 'ACCTC': 223639, 'ACCTG': 350040, 'ACCCA': 236936, 'ACCCT': 212931, 'ACCCC': 329528, 'ACCCG': 125869, 'ACCGA': 81084, 'ACCGT': 76194, 'ACCGC': 177973, 'ACCGG': 135257, 'ACGAA': 39011, 'ACGAT': 49871, 'ACGAC': 104216, 'ACGAG': 184236, 'ACGTA': 22777, 'ACGTT': 29236, 'ACGTC': 79489, 'ACGTG': 144961, 'ACGCA': 89957, 'ACGCT': 129846, 'ACGCC': 178599, 'ACGCG': 65731, 'ACGGA': 126237, 'ACGGT': 57489, 'ACGGC': 173290, 'ACGGG': 127220, 'AGAAA': 258301, 'AGAAT': 111495, 'AGAAC': 214962, 'AGAAG': 501083, 'AGATA': 56701, 'AGATT': 105444, 'AGATC': 219467, 'AGATG': 261012, 'AGACA': 186944, 'AGACT': 131189, 'AGACC': 229040, 'AGACG': 146817, 'AGAGA': 248028, 'AGAGT': 132162, 'AGAGC': 251213, 'AGAGG': 291712, 'AGTAA': 39655, 'AGTAT': 73525, 'AGTAC': 132149, 'AGTAG': 51455, 'AGTTA': 51712, 'AGTTT': 118517, 'AGTTC': 187972, 'AGTTG': 84288, 'AGTCA': 111005, 'AGTCT': 95608, 'AGTCC': 176915, 'AGTCG': 65377, 'AGTGA': 163624, 'AGTGT': 102840, 'AGTGC': 166907, 'AGTGG': 221388, 'AGCAA': 162557, 'AGCAT': 110471, 'AGCAC': 213198, 'AGCAG': 419632, 'AGCTA': 76772, 'AGCTT': 125012, 'AGCTC': 252320, 'AGCTG': 469480, 'AGCCA': 316097, 'AGCCT': 294934, 'AGCCC': 393770, 'AGCCG': 215359, 'AGCGA': 106146, 'AGCGT': 85120, 'AGCGC': 186805, 'AGCGG': 248459, 'AGGAA': 240325, 'AGGAT': 161587, 'AGGAC': 302717, 'AGGAG': 558072, 'AGGTA': 62387, 'AGGTT': 96806, 'AGGTC': 166213, 'AGGTG': 303719, 'AGGCA': 206997, 'AGGCT': 219329, 'AGGCC': 413882, 'AGGCG': 180559, 'AGGGA': 175686, 'AGGGT': 141301, 'AGGGC': 292639, 'AGGGG': 213192, 'TAAAA': 91977, 'TAAAT': 48723, 'TAAAC': 46219, 'TAAAG': 90421, 'TAATA': 22094, 'TAATT': 48999, 'TAATC': 32382, 'TAATG': 54178, 'TAACA': 56285, 'TAACT': 49650, 'TAACC': 43107, 'TAACG': 14758, 'TAAGA': 66102, 'TAAGT': 33572, 'TAAGC': 43593, 'TAAGG': 46525, 'TATAA': 36245, 'TATAT': 45602, 'TATAC': 29337, 'TATAG': 23963, 'TATTA': 37594, 'TATTT': 110982, 'TATTC': 48660, 'TATTG': 52281, 'TATCA': 52443, 'TATCT': 71897, 'TATCC': 56074, 'TATCG': 18673, 'TATGA': 88493, 'TATGT': 71291, 'TATGC': 69262, 'TATGG': 83269, 'TACAA': 138322, 'TACAT': 81833, 'TACAC': 77487, 'TACAG': 104949, 'TACTA': 47774, 'TACTT': 71590, 'TACTC': 54299, 'TACTG': 94668, 'TACCA': 113028, 'TACCT': 119851, 'TACCC': 98594, 'TACCG': 81499, 'TACGA': 85010, 'TACGT': 31934, 'TACGC': 55130, 'TACGG': 64723, 'TAGAA': 51460, 'TAGAT': 30618, 'TAGAC': 39360, 'TAGAG': 70358, 'TAGTA': 23081, 'TAGTT': 29470, 'TAGTC': 32899, 'TAGTG': 49293, 'TAGCA': 44332, 'TAGCT': 51757, 'TAGCC': 60918, 'TAGCG': 20323, 'TAGGA': 55048, 'TAGGT': 26914, 'TAGGC': 38259, 'TAGGG': 42000, 'TTAAA': 86753, 'TTAAT': 49491, 'TTAAC': 47831, 'TTAAG': 56294, 'TTATA': 38420, 'TTATT': 82844, 'TTATC': 43779, 'TTATG': 62906, 'TTACA': 65030, 'TTACT': 60903, 'TTACC': 53125, 'TTACG': 16698, 'TTAGA': 38487, 'TTAGT': 32593, 'TTAGC': 46552, 'TTAGG': 37487, 'TTTAA': 89081, 'TTTAT': 98946, 'TTTAC': 49832, 'TTTAG': 45895, 'TTTTA': 120968, 'TTTTT': 262046, 'TTTTC': 125081, 'TTTTG': 147247, 'TTTCA': 116369, 'TTTCT': 163620, 'TTTCC': 153769, 'TTTCG': 46262, 'TTTGA': 144552, 'TTTGT': 142066, 'TTTGC': 127221, 'TTTGG': 162106, 'TTCAA': 164096, 'TTCAT': 123279, 'TTCAC': 143482, 'TTCAG': 164393, 'TTCTA': 88213, 'TTCTT': 153540, 'TTCTC': 201171, 'TTCTG': 196204, 'TTCCA': 190030, 'TTCCT': 258636, 'TTCCC': 205273, 'TTCCG': 102011, 'TTCGA': 94029, 'TTCGT': 62992, 'TTCGC': 59328, 'TTCGG': 85484, 'TTGAA': 114891, 'TTGAT': 75834, 'TTGAC': 106169, 'TTGAG': 129658, 'TTGTA': 61589, 'TTGTT': 92569, 'TTGTC': 96407, 'TTGTG': 148314, 'TTGCA': 98183, 'TTGCT': 111862, 'TTGCC': 146467, 'TTGCG': 44610, 'TTGGA': 126744, 'TTGGT': 81149, 'TTGGC': 136447, 'TTGGG': 149636, 'TCAAA': 128912, 'TCAAT': 61971, 'TCAAC': 177224, 'TCAAG': 277171, 'TCATA': 41084, 'TCATT': 88842, 'TCATC': 225455, 'TCATG': 182288, 'TCACA': 121095, 'TCACT': 131631, 'TCACC': 257378, 'TCACG': 82598, 'TCAGA': 165308, 'TCAGT': 114537, 'TCAGC': 219695, 'TCAGG': 178235, 'TCTAA': 35962, 'TCTAT': 59205, 'TCTAC': 149030, 'TCTAG': 56246, 'TCTTA': 51275, 'TCTTT': 154714, 'TCTTC': 244521, 'TCTTG': 112165, 'TCTCA': 155942, 'TCTCT': 181813, 'TCTCC': 277245, 'TCTCG': 85581, 'TCTGA': 144061, 'TCTGT': 170214, 'TCTGC': 236296, 'TCTGG': 274092, 'TCCAA': 179720, 'TCCAT': 126782, 'TCCAC': 235194, 'TCCAG': 332098, 'TCCTA': 90263, 'TCCTT': 157887, 'TCCTC': 302037, 'TCCTG': 426180, 'TCCCA': 255563, 'TCCCT': 233373, 'TCCCC': 337398, 'TCCCG': 172165, 'TCCGA': 95606, 'TCCGT': 76853, 'TCCGC': 179511, 'TCCGG': 170569, 'TCGAA': 45583, 'TCGAT': 34202, 'TCGAC': 89672, 'TCGAG': 134429, 'TCGTA': 23465, 'TCGTT': 24549, 'TCGTC': 78510, 'TCGTG': 124932, 'TCGCA': 51612, 'TCGCT': 77576, 'TCGCC': 128480, 'TCGCG': 60540, 'TCGGA': 95487, 'TCGGT': 59447, 'TCGGC': 144908, 'TCGGG': 134287, 'TGAAA': 138925, 'TGAAT': 86095, 'TGAAC': 158210, 'TGAAG': 353024, 'TGATA': 48081, 'TGATT': 76408, 'TGATC': 133580, 'TGATG': 211124, 'TGACA': 157453, 'TGACT': 112140, 'TGACC': 251660, 'TGACG': 97259, 'TGAGA': 164412, 'TGAGT': 117821, 'TGAGC': 240770, 'TGAGG': 285798, 'TGTAA': 70503, 'TGTAT': 67472, 'TGTAC': 93441, 'TGTAG': 55250, 'TGTTA': 55370, 'TGTTT': 140067, 'TGTTC': 137635, 'TGTTG': 111099, 'TGTCA': 133571, 'TGTCT': 146168, 'TGTCC': 220536, 'TGTCG': 66873, 'TGTGA': 183143, 'TGTGT': 170141, 'TGTGC': 214126, 'TGTGG': 288872, 'TGCAA': 126333, 'TGCAT': 91947, 'TGCAC': 161397, 'TGCAG': 375975, 'TGCTA': 64994, 'TGCTT': 126424, 'TGCTC': 193374, 'TGCTG': 470550, 'TGCCA': 261190, 'TGCCT': 280410, 'TGCCC': 378210, 'TGCCG': 175381, 'TGCGA': 72669, 'TGCGT': 74284, 'TGCGC': 167002, 'TGCGG': 174368, 'TGGAA': 190250, 'TGGAT': 156631, 'TGGAC': 310688, 'TGGAG': 504434, 'TGGTA': 66742, 'TGGTT': 97750, 'TGGTC': 189281, 'TGGTG': 353734, 'TGGCA': 224741, 'TGGCT': 268989, 'TGGCC': 459306, 'TGGCG': 190786, 'TGGGA': 249838, 'TGGGT': 166824, 'TGGGC': 368069, 'TGGGG': 328961, 'CAAAA': 159044, 'CAAAT': 101154, 'CAAAC': 124632, 'CAAAG': 205460, 'CAATA': 45582, 'CAATT': 53576, 'CAATC': 61233, 'CAATG': 117183, 'CAACA': 235024, 'CAACT': 126481, 'CAACC': 201180, 'CAACG': 121076, 'CAAGA': 393541, 'CAAGT': 195945, 'CAAGC': 271755, 'CAAGG': 384696, 'CATAA': 42125, 'CATAT': 37356, 'CATAC': 48252, 'CATAG': 41212, 'CATTA': 52111, 'CATTT': 96821, 'CATTC': 98131, 'CATTG': 137209, 'CATCA': 271364, 'CATCT': 222780, 'CATCC': 284186, 'CATCG': 165628, 'CATGA': 192204, 'CATGT': 142379, 'CATGC': 174004, 'CATGG': 281034, 'CACAA': 141933, 'CACAT': 108786, 'CACAC': 162839, 'CACAG': 262397, 'CACTA': 69967, 'CACTT': 120757, 'CACTC': 130670, 'CACTG': 231697, 'CACCA': 340620, 'CACCT': 291173, 'CACCC': 345995, 'CACCG': 182352, 'CACGA': 88376, 'CACGT': 82623, 'CACGC': 178012, 'CACGG': 164776, 'CAGAA': 264077, 'CAGAT': 185047, 'CAGAC': 221144, 'CAGAG': 330901, 'CAGTA': 87307, 'CAGTT': 137567, 'CAGTC': 131994, 'CAGTG': 276448, 'CAGCA': 398936, 'CAGCT': 357052, 'CAGCC': 551517, 'CAGCG': 255723, 'CAGGA': 333542, 'CAGGT': 214328, 'CAGGC': 357076, 'CAGGG': 291437, 'CTAAA': 62376, 'CTAAT': 34438, 'CTAAC': 33009, 'CTAAG': 60188, 'CTATA': 35496, 'CTATT': 57993, 'CTATC': 64104, 'CTATG': 120987, 'CTACA': 175236, 'CTACT': 94006, 'CTACC': 196726, 'CTACG': 146256, 'CTAGA': 53246, 'CTAGT': 34618, 'CTAGC': 50241, 'CTAGG': 49797, 'CTTAA': 51896, 'CTTAT': 50310, 'CTTAC': 43252, 'CTTAG': 50284, 'CTTTA': 55653, 'CTTTT': 151371, 'CTTTC': 153995, 'CTTTG': 198114, 'CTTCA': 258084, 'CTTCT': 246470, 'CTTCC': 360805, 'CTTCG': 138394, 'CTTGA': 106079, 'CTTGT': 97838, 'CTTGC': 116560, 'CTTGG': 155688, 'CTCAA': 190983, 'CTCAT': 156053, 'CTCAC': 199048, 'CTCAG': 259336, 'CTCTA': 83591, 'CTCTT': 162917, 'CTCTC': 213391, 'CTCTG': 293786, 'CTCCA': 337988, 'CTCCT': 334046, 'CTCCC': 390979, 'CTCCG': 194572, 'CTCGA': 64396, 'CTCGT': 61228, 'CTCGC': 112461, 'CTCGG': 187870, 'CTGAA': 242027, 'CTGAT': 144374, 'CTGAC': 199475, 'CTGAG': 301499, 'CTGTA': 101828, 'CTGTT': 141766, 'CTGTC': 210282, 'CTGTG': 322125, 'CTGCA': 349072, 'CTGCT': 409145, 'CTGCC': 517846, 'CTGCG': 246371, 'CTGGA': 505838, 'CTGGT': 271897, 'CTGGC': 455424, 'CTGGG': 496114, 'CCAAA': 171218, 'CCAAT': 83404, 'CCAAC': 221779, 'CCAAG': 402494, 'CCATA': 59887, 'CCATT': 116285, 'CCATC': 314633, 'CCATG': 284892, 'CCACA': 250784, 'CCACT': 194908, 'CCACC': 463978, 'CCACG': 208737, 'CCAGA': 361449, 'CCAGT': 215747, 'CCAGC': 568663, 'CCAGG': 484573, 'CCTAA': 59652, 'CCTAT': 88208, 'CCTAC': 190490, 'CCTAG': 64562, 'CCTTA': 63079, 'CCTTT': 154960, 'CCTTC': 332630, 'CCTTG': 165801, 'CCTCA': 316039, 'CCTCT': 272587, 'CCTCC': 487302, 'CCTCG': 178574, 'CCTGA': 302660, 'CCTGT': 232930, 'CCTGC': 564304, 'CCTGG': 698653, 'CCCAA': 238111, 'CCCAT': 182671, 'CCCAC': 351487, 'CCCAG': 576506, 'CCCTA': 115563, 'CCCTT': 204503, 'CCCTC': 350780, 'CCCTG': 540863, 'CCCCA': 476718, 'CCCCT': 379431, 'CCCCC': 583877, 'CCCCG': 333810, 'CCCGA': 188436, 'CCCGT': 120813, 'CCCGC': 300559, 'CCCGG': 345997, 'CCGAA': 75076, 'CCGAT': 53150, 'CCGAC': 159180, 'CCGAG': 271958, 'CCGTA': 34084, 'CCGTT': 47420, 'CCGTC': 136629, 'CCGTG': 187879, 'CCGCA': 210328, 'CCGCT': 200966, 'CCGCC': 435829, 'CCGCG': 207728, 'CCGGA': 206539, 'CCGGT': 76060, 'CCGGC': 314918, 'CCGGG': 345031, 'CGAAA': 38631, 'CGAAT': 38681, 'CGAAC': 47963, 'CGAAG': 83119, 'CGATA': 21651, 'CGATT': 35949, 'CGATC': 37983, 'CGATG': 93181, 'CGACA': 118206, 'CGACT': 84373, 'CGACC': 144055, 'CGACG': 113138, 'CGAGA': 184343, 'CGAGT': 111417, 'CGAGC': 192669, 'CGAGG': 292849, 'CGTAA': 22812, 'CGTAT': 17116, 'CGTAC': 36159, 'CGTAG': 23862, 'CGTTA': 11293, 'CGTTT': 38275, 'CGTTC': 46799, 'CGTTG': 46756, 'CGTCA': 114855, 'CGTCT': 110275, 'CGTCC': 135394, 'CGTCG': 54075, 'CGTGA': 100453, 'CGTGT': 93683, 'CGTGC': 174707, 'CGTGG': 258894, 'CGCAA': 128587, 'CGCAT': 100799, 'CGCAC': 111926, 'CGCAG': 169924, 'CGCTA': 69965, 'CGCTT': 94841, 'CGCTC': 141084, 'CGCTG': 277536, 'CGCCA': 252881, 'CGCCT': 239669, 'CGCCC': 289108, 'CGCCG': 245762, 'CGCGA': 83287, 'CGCGT': 65462, 'CGCGC': 161473, 'CGCGG': 184835, 'CGGAA': 146223, 'CGGAT': 72042, 'CGGAC': 140497, 'CGGAG': 276055, 'CGGTA': 28406, 'CGGTT': 44982, 'CGGTC': 76399, 'CGGTG': 128519, 'CGGCA': 210130, 'CGGCT': 201674, 'CGGCC': 382792, 'CGGCG': 266087, 'CGGGA': 221048, 'CGGGT': 101101, 'CGGGC': 275407, 'CGGGG': 269955, 'GAAAA': 173468, 'GAAAT': 106128, 'GAAAC': 149192, 'GAAAG': 180056, 'GAATA': 56477, 'GAATT': 81012, 'GAATC': 74545, 'GAATG': 122876, 'GAACA': 168262, 'GAACT': 134443, 'GAACC': 168935, 'GAACG': 101985, 'GAAGA': 447312, 'GAAGT': 172043, 'GAAGC': 327112, 'GAAGG': 337114, 'GATAA': 51049, 'GATAT': 47412, 'GATAC': 43001, 'GATAG': 36035, 'GATTA': 46050, 'GATTT': 87857, 'GATTC': 97072, 'GATTG': 83571, 'GATCA': 142442, 'GATCT': 111111, 'GATCC': 187306, 'GATCG': 76031, 'GATGA': 285532, 'GATGT': 125258, 'GATGC': 162401, 'GATGG': 230751, 'GACAA': 205591, 'GACAT': 167218, 'GACAC': 150684, 'GACAG': 226055, 'GACTA': 65662, 'GACTT': 126421, 'GACTC': 164673, 'GACTG': 167865, 'GACCA': 293447, 'GACCT': 237215, 'GACCC': 295292, 'GACCG': 136352, 'GACGA': 137112, 'GACGT': 91447, 'GACGC': 156193, 'GACGG': 167921, 'GAGAA': 358264, 'GAGAT': 194930, 'GAGAC': 221821, 'GAGAG': 253697, 'GAGTA': 88114, 'GAGTT': 131010, 'GAGTC': 145360, 'GAGTG': 187165, 'GAGCA': 265632, 'GAGCT': 294563, 'GAGCC': 361023, 'GAGCG': 224774, 'GAGGA': 561407, 'GAGGT': 214012, 'GAGGC': 390516, 'GAGGG': 294352, 'GTAAA': 54057, 'GTAAT': 39101, 'GTAAC': 43242, 'GTAAG': 34666, 'GTATA': 23353, 'GTATT': 55481, 'GTATC': 43039, 'GTATG': 82234, 'GTACA': 100573, 'GTACT': 66836, 'GTACC': 113441, 'GTACG': 50661, 'GTAGA': 58726, 'GTAGT': 34609, 'GTAGC': 49858, 'GTAGG': 38503, 'GTTAA': 40649, 'GTTAT': 34800, 'GTTAC': 42438, 'GTTAG': 33085, 'GTTTA': 45141, 'GTTTT': 104517, 'GTTTC': 96017, 'GTTTG': 145858, 'GTTCA': 131058, 'GTTCT': 137165, 'GTTCC': 147384, 'GTTCG': 73077, 'GTTGA': 65024, 'GTTGT': 78388, 'GTTGC': 80505, 'GTTGG': 110703, 'GTCAA': 120009, 'GTCAT': 112866, 'GTCAC': 137046, 'GTCAG': 152057, 'GTCTA': 62950, 'GTCTT': 112592, 'GTCTC': 153390, 'GTCTG': 182306, 'GTCCA': 183669, 'GTCCT': 203620, 'GTCCC': 242917, 'GTCCG': 103171, 'GTCGA': 48711, 'GTCGT': 67977, 'GTCGC': 77985, 'GTCGG': 95793, 'GTGAA': 198732, 'GTGAT': 103044, 'GTGAC': 177222, 'GTGAG': 172367, 'GTGTA': 62613, 'GTGTT': 118062, 'GTGTC': 135507, 'GTGTG': 218827, 'GTGCA': 184999, 'GTGCT': 208637, 'GTGCC': 250188, 'GTGCG': 136727, 'GTGGA': 334936, 'GTGGT': 220198, 'GTGGC': 335399, 'GTGGG': 292197, 'GCAAA': 130263, 'GCAAT': 68020, 'GCAAC': 126480, 'GCAAG': 277548, 'GCATA': 28994, 'GCATT': 85816, 'GCATC': 190898, 'GCATG': 152795, 'GCACA': 156312, 'GCACT': 134768, 'GCACC': 270368, 'GCACG': 134912, 'GCAGA': 284761, 'GCAGT': 184982, 'GCAGC': 514369, 'GCAGG': 350988, 'GCTAA': 51333, 'GCTAT': 75938, 'GCTAC': 153016, 'GCTAG': 44870, 'GCTTA': 40667, 'GCTTT': 131262, 'GCTTC': 238383, 'GCTTG': 107416, 'GCTCA': 225831, 'GCTCT': 194956, 'GCTCC': 314260, 'GCTCG': 100015, 'GCTGA': 305214, 'GCTGT': 249101, 'GCTGC': 542312, 'GCTGG': 556595, 'GCCAA': 256576, 'GCCAT': 275094, 'GCCAC': 340977, 'GCCAG': 404714, 'GCCTA': 111699, 'GCCTT': 220983, 'GCCTC': 376950, 'GCCTG': 479583, 'GCCCA': 381094, 'GCCCT': 386473, 'GCCCC': 531532, 'GCCCG': 323576, 'GCCGA': 193973, 'GCCGT': 132556, 'GCCGC': 395448, 'GCCGG': 289233, 'GCGAA': 48588, 'GCGAT': 51461, 'GCGAC': 106216, 'GCGAG': 190573, 'GCGTA': 19458, 'GCGTT': 41477, 'GCGTC': 120350, 'GCGTG': 171137, 'GCGCA': 159137, 'GCGCT': 174657, 'GCGCC': 285632, 'GCGCG': 159559, 'GCGGA': 202936, 'GCGGT': 84953, 'GCGGC': 425923, 'GCGGG': 261056, 'GGAAA': 172216, 'GGAAT': 98873, 'GGAAC': 153064, 'GGAAG': 349530, 'GGATA': 51222, 'GGATT': 97211, 'GGATC': 121823, 'GGATG': 238714, 'GGACA': 286926, 'GGACT': 197083, 'GGACC': 339944, 'GGACG': 195810, 'GGAGA': 431569, 'GGAGT': 191730, 'GGAGC': 458477, 'GGAGG': 588924, 'GGTAA': 38569, 'GGTAT': 46507, 'GGTAC': 70291, 'GGTAG': 51131, 'GGTTA': 32966, 'GGTTT': 95713, 'GGTTC': 116182, 'GGTTG': 91495, 'GGTCA': 161121, 'GGTCT': 158486, 'GGTCC': 201124, 'GGTCG': 103611, 'GGTGA': 203008, 'GGTGT': 168917, 'GGTGC': 225586, 'GGTGG': 415102, 'GGCAA': 185469, 'GGCAT': 155514, 'GGCAC': 208769, 'GGCAG': 370560, 'GGCTA': 113397, 'GGCTT': 171981, 'GGCTC': 250983, 'GGCTG': 438354, 'GGCCA': 448666, 'GGCCT': 377996, 'GGCCC': 564362, 'GGCCG': 375111, 'GGCGA': 133426, 'GGCGT': 127977, 'GGCGC': 263248, 'GGCGG': 363875, 'GGGAA': 197310, 'GGGAT': 119054, 'GGGAC': 266556, 'GGGAG': 329702, 'GGGTA': 49693, 'GGGTT': 96826, 'GGGTC': 191526, 'GGGTG': 225220, 'GGGCA': 280231, 'GGGCT': 284217, 'GGGCC': 510322, 'GGGCG': 246830, 'GGGGA': 267463, 'GGGGT': 154219, 'GGGGC': 388643, 'GGGGG': 272783}, 'overrepresented_sequences': {}} {'passed_filter_reads': 6126216, 'low_quality_reads': 137664, 'too_many_N_reads': 1422, 'too_short_reads': 78, 'too_long_reads': 0} fastp -i /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045017_1.fastq.gz -I /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045017_2.fastq.gz -o /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045017.trimmed_1.fastq.gz -O /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045017.trimmed_2.fastq.gz -l 25 --adapter_fasta /home/ubuntu/workspace/rnaseq/team_exercise/references/illumina_multiplex.fa --trim_tail1 5 --trim_tail2 5 --json /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045017.fastp.json --html /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045017.fastp.html SRR10045018_1 {'adapter_trimmed_reads': 35010, 'adapter_trimmed_bases': 224173, 'read1_adapter_sequence': 'unspecified', 'read2_adapter_sequence': 'unspecified', 'read1_adapter_counts': {'A': 529, 'C': 637, 'G': 642, 'T': 545, 'CC': 182, 'GG': 231, 'others': 14738}, 'read2_adapter_counts': {'A': 618, 'C': 655, 'G': 602, 'T': 479, 'CC': 203, 'others': 14948}} {'rate': 0.0717084} {'peak': 85, 'unknown': 898535, 'histogram': [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 39, 47, 34, 35, 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'ACCGG': 146734, 'ACGAA': 43599, 'ACGAT': 51989, 'ACGAC': 110359, 'ACGAG': 190607, 'ACGTA': 23807, 'ACGTT': 31597, 'ACGTC': 84744, 'ACGTG': 149438, 'ACGCA': 94152, 'ACGCT': 132919, 'ACGCC': 191016, 'ACGCG': 69300, 'ACGGA': 130970, 'ACGGT': 60223, 'ACGGC': 181962, 'ACGGG': 136247, 'AGAAA': 271748, 'AGAAT': 115849, 'AGAAC': 223886, 'AGAAG': 517065, 'AGATA': 58357, 'AGATT': 111526, 'AGATC': 235943, 'AGATG': 268366, 'AGACA': 196671, 'AGACT': 135089, 'AGACC': 242209, 'AGACG': 153852, 'AGAGA': 254279, 'AGAGT': 135027, 'AGAGC': 260921, 'AGAGG': 307554, 'AGTAA': 43189, 'AGTAT': 75305, 'AGTAC': 136649, 'AGTAG': 54663, 'AGTTA': 54615, 'AGTTT': 126027, 'AGTTC': 196305, 'AGTTG': 86654, 'AGTCA': 115254, 'AGTCT': 98750, 'AGTCC': 187678, 'AGTCG': 68217, 'AGTGA': 170001, 'AGTGT': 107737, 'AGTGC': 174774, 'AGTGG': 230319, 'AGCAA': 172385, 'AGCAT': 115897, 'AGCAC': 222284, 'AGCAG': 431320, 'AGCTA': 81093, 'AGCTT': 132979, 'AGCTC': 259866, 'AGCTG': 487111, 'AGCCA': 326560, 'AGCCT': 306760, 'AGCCC': 436238, 'AGCCG': 229664, 'AGCGA': 111275, 'AGCGT': 88665, 'AGCGC': 194357, 'AGCGG': 259920, 'AGGAA': 251451, 'AGGAT': 167437, 'AGGAC': 316759, 'AGGAG': 576376, 'AGGTA': 65076, 'AGGTT': 104153, 'AGGTC': 176013, 'AGGTG': 311422, 'AGGCA': 217959, 'AGGCT': 229950, 'AGGCC': 441450, 'AGGCG': 192067, 'AGGGA': 185921, 'AGGGT': 149106, 'AGGGC': 308612, 'AGGGG': 234651, 'TAAAA': 98144, 'TAAAT': 52492, 'TAAAC': 50513, 'TAAAG': 97546, 'TAATA': 23970, 'TAATT': 53207, 'TAATC': 34631, 'TAATG': 58015, 'TAACA': 58648, 'TAACT': 52244, 'TAACC': 47744, 'TAACG': 16964, 'TAAGA': 69646, 'TAAGT': 35933, 'TAAGC': 47788, 'TAAGG': 50198, 'TATAA': 38256, 'TATAT': 48173, 'TATAC': 31094, 'TATAG': 25183, 'TATTA': 40220, 'TATTT': 119245, 'TATTC': 51435, 'TATTG': 54663, 'TATCA': 56143, 'TATCT': 74243, 'TATCC': 60689, 'TATCG': 19346, 'TATGA': 92912, 'TATGT': 75175, 'TATGC': 72751, 'TATGG': 87986, 'TACAA': 144246, 'TACAT': 84951, 'TACAC': 81771, 'TACAG': 111851, 'TACTA': 49120, 'TACTT': 76765, 'TACTC': 57919, 'TACTG': 99275, 'TACCA': 118017, 'TACCT': 126415, 'TACCC': 108448, 'TACCG': 86974, 'TACGA': 87112, 'TACGT': 33698, 'TACGC': 58232, 'TACGG': 67743, 'TAGAA': 54077, 'TAGAT': 32738, 'TAGAC': 42131, 'TAGAG': 73308, 'TAGTA': 24501, 'TAGTT': 32002, 'TAGTC': 34388, 'TAGTG': 50300, 'TAGCA': 46879, 'TAGCT': 55292, 'TAGCC': 66249, 'TAGCG': 22285, 'TAGGA': 58489, 'TAGGT': 29365, 'TAGGC': 41882, 'TAGGG': 46291, 'TTAAA': 92852, 'TTAAT': 54621, 'TTAAC': 50755, 'TTAAG': 61033, 'TTATA': 41375, 'TTATT': 89295, 'TTATC': 47122, 'TTATG': 66498, 'TTACA': 70847, 'TTACT': 63662, 'TTACC': 57410, 'TTACG': 18747, 'TTAGA': 40195, 'TTAGT': 35044, 'TTAGC': 50806, 'TTAGG': 41507, 'TTTAA': 96442, 'TTTAT': 105895, 'TTTAC': 53556, 'TTTAG': 49771, 'TTTTA': 127475, 'TTTTT': 274180, 'TTTTC': 132634, 'TTTTG': 157998, 'TTTCA': 121054, 'TTTCT': 170640, 'TTTCC': 168655, 'TTTCG': 49457, 'TTTGA': 153112, 'TTTGT': 149239, 'TTTGC': 136463, 'TTTGG': 176752, 'TTCAA': 171565, 'TTCAT': 130082, 'TTCAC': 152355, 'TTCAG': 170550, 'TTCTA': 92876, 'TTCTT': 158929, 'TTCTC': 208047, 'TTCTG': 206874, 'TTCCA': 201135, 'TTCCT': 273552, 'TTCCC': 228879, 'TTCCG': 110178, 'TTCGA': 97903, 'TTCGT': 66040, 'TTCGC': 63353, 'TTCGG': 91552, 'TTGAA': 121795, 'TTGAT': 80643, 'TTGAC': 112544, 'TTGAG': 136317, 'TTGTA': 65359, 'TTGTT': 97706, 'TTGTC': 99668, 'TTGTG': 155831, 'TTGCA': 104942, 'TTGCT': 118237, 'TTGCC': 160171, 'TTGCG': 46989, 'TTGGA': 135264, 'TTGGT': 86202, 'TTGGC': 145590, 'TTGGG': 160433, 'TCAAA': 134587, 'TCAAT': 65034, 'TCAAC': 185220, 'TCAAG': 289004, 'TCATA': 43440, 'TCATT': 93464, 'TCATC': 234852, 'TCATG': 191355, 'TCACA': 125223, 'TCACT': 136781, 'TCACC': 270919, 'TCACG': 86955, 'TCAGA': 172175, 'TCAGT': 117462, 'TCAGC': 227148, 'TCAGG': 186202, 'TCTAA': 38427, 'TCTAT': 61569, 'TCTAC': 154744, 'TCTAG': 58988, 'TCTTA': 55317, 'TCTTT': 158926, 'TCTTC': 255000, 'TCTTG': 116007, 'TCTCA': 160135, 'TCTCT': 187328, 'TCTCC': 291201, 'TCTCG': 88265, 'TCTGA': 148486, 'TCTGT': 175627, 'TCTGC': 245835, 'TCTGG': 284383, 'TCCAA': 189903, 'TCCAT': 132665, 'TCCAC': 246136, 'TCCAG': 348109, 'TCCTA': 95323, 'TCCTT': 166472, 'TCCTC': 316052, 'TCCTG': 447088, 'TCCCA': 270572, 'TCCCT': 249133, 'TCCCC': 389027, 'TCCCG': 187509, 'TCCGA': 103043, 'TCCGT': 81239, 'TCCGC': 192143, 'TCCGG': 184995, 'TCGAA': 47509, 'TCGAT': 36262, 'TCGAC': 94773, 'TCGAG': 140473, 'TCGTA': 25183, 'TCGTT': 26141, 'TCGTC': 81559, 'TCGTG': 131506, 'TCGCA': 54978, 'TCGCT': 79984, 'TCGCC': 138527, 'TCGCG': 63045, 'TCGGA': 97082, 'TCGGT': 62464, 'TCGGC': 152574, 'TCGGG': 142106, 'TGAAA': 148829, 'TGAAT': 90914, 'TGAAC': 164912, 'TGAAG': 369279, 'TGATA': 50814, 'TGATT': 80783, 'TGATC': 139181, 'TGATG': 224372, 'TGACA': 164273, 'TGACT': 118655, 'TGACC': 268073, 'TGACG': 101451, 'TGAGA': 171555, 'TGAGT': 121555, 'TGAGC': 249243, 'TGAGG': 298164, 'TGTAA': 75346, 'TGTAT': 71221, 'TGTAC': 99382, 'TGTAG': 57692, 'TGTTA': 59288, 'TGTTT': 147573, 'TGTTC': 143068, 'TGTTG': 117393, 'TGTCA': 136617, 'TGTCT': 150638, 'TGTCC': 231865, 'TGTCG': 70148, 'TGTGA': 190322, 'TGTGT': 175832, 'TGTGC': 222096, 'TGTGG': 302830, 'TGCAA': 133320, 'TGCAT': 96697, 'TGCAC': 168109, 'TGCAG': 388013, 'TGCTA': 69724, 'TGCTT': 134137, 'TGCTC': 199315, 'TGCTG': 485854, 'TGCCA': 272475, 'TGCCT': 295347, 'TGCCC': 414462, 'TGCCG': 187495, 'TGCGA': 75613, 'TGCGT': 77219, 'TGCGC': 174186, 'TGCGG': 185168, 'TGGAA': 199377, 'TGGAT': 163497, 'TGGAC': 323732, 'TGGAG': 523236, 'TGGTA': 69934, 'TGGTT': 104963, 'TGGTC': 197996, 'TGGTG': 368733, 'TGGCA': 235306, 'TGGCT': 279134, 'TGGCC': 487514, 'TGGCG': 203884, 'TGGGA': 264015, 'TGGGT': 174579, 'TGGGC': 386638, 'TGGGG': 354031, 'CAAAA': 172137, 'CAAAT': 106995, 'CAAAC': 134009, 'CAAAG': 219839, 'CAATA': 49477, 'CAATT': 57871, 'CAATC': 65163, 'CAATG': 124081, 'CAACA': 247726, 'CAACT': 131191, 'CAACC': 220531, 'CAACG': 127583, 'CAAGA': 409811, 'CAAGT': 203410, 'CAAGC': 287239, 'CAAGG': 398817, 'CATAA': 45303, 'CATAT': 39862, 'CATAC': 51269, 'CATAG': 43590, 'CATTA': 55955, 'CATTT': 104196, 'CATTC': 103702, 'CATTG': 144523, 'CATCA': 281947, 'CATCT': 232014, 'CATCC': 301823, 'CATCG': 173541, 'CATGA': 197881, 'CATGT': 148790, 'CATGC': 183930, 'CATGG': 295448, 'CACAA': 147900, 'CACAT': 111790, 'CACAC': 172642, 'CACAG': 273614, 'CACTA': 72765, 'CACTT': 129027, 'CACTC': 137281, 'CACTG': 240474, 'CACCA': 357580, 'CACCT': 302576, 'CACCC': 390109, 'CACCG': 194438, 'CACGA': 94539, 'CACGT': 85788, 'CACGC': 185199, 'CACGG': 174463, 'CAGAA': 275961, 'CAGAT': 196449, 'CAGAC': 232710, 'CAGAG': 343970, 'CAGTA': 90576, 'CAGTT': 145634, 'CAGTC': 137093, 'CAGTG': 288150, 'CAGCA': 411915, 'CAGCT': 364269, 'CAGCC': 585890, 'CAGCG': 266822, 'CAGGA': 344564, 'CAGGT': 224466, 'CAGGC': 378804, 'CAGGG': 312334, 'CTAAA': 66843, 'CTAAT': 35937, 'CTAAC': 36234, 'CTAAG': 63880, 'CTATA': 36969, 'CTATT': 62039, 'CTATC': 67711, 'CTATG': 126872, 'CTACA': 181386, 'CTACT': 97804, 'CTACC': 206798, 'CTACG': 148613, 'CTAGA': 56894, 'CTAGT': 36564, 'CTAGC': 53923, 'CTAGG': 54201, 'CTTAA': 55986, 'CTTAT': 53937, 'CTTAC': 48449, 'CTTAG': 54274, 'CTTTA': 60432, 'CTTTT': 159380, 'CTTTC': 162014, 'CTTTG': 210179, 'CTTCA': 268406, 'CTTCT': 255297, 'CTTCC': 389596, 'CTTCG': 146092, 'CTTGA': 112002, 'CTTGT': 103109, 'CTTGC': 125941, 'CTTGG': 165026, 'CTCAA': 197228, 'CTCAT': 160933, 'CTCAC': 208966, 'CTCAG': 265485, 'CTCTA': 85602, 'CTCTT': 169447, 'CTCTC': 222728, 'CTCTG': 301569, 'CTCCA': 350113, 'CTCCT': 346378, 'CTCCC': 435411, 'CTCCG': 211818, 'CTCGA': 68555, 'CTCGT': 62973, 'CTCGC': 119515, 'CTCGG': 193360, 'CTGAA': 254954, 'CTGAT': 152057, 'CTGAC': 210842, 'CTGAG': 312452, 'CTGTA': 107097, 'CTGTT': 147944, 'CTGTC': 218286, 'CTGTG': 333622, 'CTGCA': 357273, 'CTGCT': 419901, 'CTGCC': 557983, 'CTGCG': 259344, 'CTGGA': 521398, 'CTGGT': 282670, 'CTGGC': 484911, 'CTGGG': 531479, 'CCAAA': 185617, 'CCAAT': 89934, 'CCAAC': 239645, 'CCAAG': 424999, 'CCATA': 64212, 'CCATT': 124865, 'CCATC': 331954, 'CCATG': 298198, 'CCACA': 261187, 'CCACT': 203365, 'CCACC': 507097, 'CCACG': 220459, 'CCAGA': 377370, 'CCAGT': 228152, 'CCAGC': 600351, 'CCAGG': 512398, 'CCTAA': 63730, 'CCTAT': 93752, 'CCTAC': 199906, 'CCTAG': 70785, 'CCTTA': 68989, 'CCTTT': 168880, 'CCTTC': 357050, 'CCTTG': 178140, 'CCTCA': 326151, 'CCTCT': 281356, 'CCTCC': 532132, 'CCTCG': 187650, 'CCTGA': 317444, 'CCTGT': 244802, 'CCTGC': 598812, 'CCTGG': 739701, 'CCCAA': 261561, 'CCCAT': 197217, 'CCCAC': 390812, 'CCCAG': 617690, 'CCCTA': 125779, 'CCCTT': 228629, 'CCCTC': 383499, 'CCCTG': 584006, 'CCCCA': 530307, 'CCCCT': 425163, 'CCCCC': 845637, 'CCCCG': 390946, 'CCCGA': 203445, 'CCCGT': 133283, 'CCCGC': 343049, 'CCCGG': 388741, 'CCGAA': 82395, 'CCGAT': 57676, 'CCGAC': 171790, 'CCGAG': 287201, 'CCGTA': 37499, 'CCGTT': 52389, 'CCGTC': 146864, 'CCGTG': 201118, 'CCGCA': 222909, 'CCGCT': 212421, 'CCGCC': 487054, 'CCGCG': 223507, 'CCGGA': 219510, 'CCGGT': 84056, 'CCGGC': 347073, 'CCGGG': 381636, 'CGAAA': 42779, 'CGAAT': 41534, 'CGAAC': 52278, 'CGAAG': 89632, 'CGATA': 23173, 'CGATT': 39081, 'CGATC': 41479, 'CGATG': 97259, 'CGACA': 123600, 'CGACT': 87249, 'CGACC': 156485, 'CGACG': 117546, 'CGAGA': 188517, 'CGAGT': 116138, 'CGAGC': 203171, 'CGAGG': 307930, 'CGTAA': 24252, 'CGTAT': 18201, 'CGTAC': 40405, 'CGTAG': 25858, 'CGTTA': 12627, 'CGTTT': 42287, 'CGTTC': 50801, 'CGTTG': 50509, 'CGTCA': 120100, 'CGTCT': 113748, 'CGTCC': 146220, 'CGTCG': 57643, 'CGTGA': 105019, 'CGTGT': 97487, 'CGTGC': 182444, 'CGTGG': 273925, 'CGCAA': 134946, 'CGCAT': 105214, 'CGCAC': 121706, 'CGCAG': 176857, 'CGCTA': 72062, 'CGCTT': 100240, 'CGCTC': 149089, 'CGCTG': 287280, 'CGCCA': 262324, 'CGCCT': 248143, 'CGCCC': 341647, 'CGCCG': 263914, 'CGCGA': 86380, 'CGCGT': 68439, 'CGCGC': 172067, 'CGCGG': 196403, 'CGGAA': 152613, 'CGGAT': 75279, 'CGGAC': 150111, 'CGGAG': 286536, 'CGGTA': 31407, 'CGGTT': 48981, 'CGGTC': 83202, 'CGGTG': 136186, 'CGGCA': 219928, 'CGGCT': 208407, 'CGGCC': 418367, 'CGGCG': 281647, 'CGGGA': 233592, 'CGGGT': 107356, 'CGGGC': 296956, 'CGGGG': 299998, 'GAAAA': 187125, 'GAAAT': 112798, 'GAAAC': 156586, 'GAAAG': 188946, 'GAATA': 58470, 'GAATT': 85640, 'GAATC': 78324, 'GAATG': 131141, 'GAACA': 176135, 'GAACT': 140199, 'GAACC': 180210, 'GAACG': 105424, 'GAAGA': 460650, 'GAAGT': 179477, 'GAAGC': 345015, 'GAAGG': 355010, 'GATAA': 54265, 'GATAT': 48698, 'GATAC': 47016, 'GATAG': 38683, 'GATTA': 49698, 'GATTT': 94155, 'GATTC': 102180, 'GATTG': 88045, 'GATCA': 147605, 'GATCT': 114392, 'GATCC': 200189, 'GATCG': 81225, 'GATGA': 295694, 'GATGT': 130891, 'GATGC': 172408, 'GATGG': 240276, 'GACAA': 217139, 'GACAT': 173757, 'GACAC': 160077, 'GACAG': 236886, 'GACTA': 68393, 'GACTT': 131877, 'GACTC': 172387, 'GACTG': 175762, 'GACCA': 306000, 'GACCT': 246347, 'GACCC': 320982, 'GACCG': 149075, 'GACGA': 143237, 'GACGT': 94707, 'GACGC': 165100, 'GACGG': 175577, 'GAGAA': 366994, 'GAGAT': 201767, 'GAGAC': 232311, 'GAGAG': 260807, 'GAGTA': 89995, 'GAGTT': 136626, 'GAGTC': 152151, 'GAGTG': 193683, 'GAGCA': 274980, 'GAGCT': 301219, 'GAGCC': 386056, 'GAGCG': 232212, 'GAGGA': 577695, 'GAGGT': 221314, 'GAGGC': 412141, 'GAGGG': 319724, 'GTAAA': 58813, 'GTAAT': 41917, 'GTAAC': 46754, 'GTAAG': 37633, 'GTATA': 25028, 'GTATT': 57359, 'GTATC': 45824, 'GTATG': 85235, 'GTACA': 103918, 'GTACT': 71105, 'GTACC': 121496, 'GTACG': 54824, 'GTAGA': 61138, 'GTAGT': 35052, 'GTAGC': 53581, 'GTAGG': 41959, 'GTTAA': 44809, 'GTTAT': 37343, 'GTTAC': 45361, 'GTTAG': 36339, 'GTTTA': 48942, 'GTTTT': 112973, 'GTTTC': 104025, 'GTTTG': 154189, 'GTTCA': 136535, 'GTTCT': 143250, 'GTTCC': 157858, 'GTTCG': 77059, 'GTTGA': 68721, 'GTTGT': 81153, 'GTTGC': 86711, 'GTTGG': 116956, 'GTCAA': 125488, 'GTCAT': 117590, 'GTCAC': 142035, 'GTCAG': 160489, 'GTCTA': 65338, 'GTCTT': 117556, 'GTCTC': 158956, 'GTCTG': 187043, 'GTCCA': 191051, 'GTCCT': 213198, 'GTCCC': 264673, 'GTCCG': 110892, 'GTCGA': 51694, 'GTCGT': 70395, 'GTCGC': 81547, 'GTCGG': 101805, 'GTGAA': 209783, 'GTGAT': 107655, 'GTGAC': 186295, 'GTGAG': 178091, 'GTGTA': 65564, 'GTGTT': 123628, 'GTGTC': 141659, 'GTGTG': 226523, 'GTGCA': 191710, 'GTGCT': 214215, 'GTGCC': 264185, 'GTGCG': 142568, 'GTGGA': 344508, 'GTGGT': 228253, 'GTGGC': 353996, 'GTGGG': 311395, 'GCAAA': 140822, 'GCAAT': 73029, 'GCAAC': 135134, 'GCAAG': 290624, 'GCATA': 30978, 'GCATT': 91189, 'GCATC': 201276, 'GCATG': 159538, 'GCACA': 161595, 'GCACT': 139787, 'GCACC': 290073, 'GCACG': 139179, 'GCAGA': 295847, 'GCAGT': 190272, 'GCAGC': 529944, 'GCAGG': 370665, 'GCTAA': 56223, 'GCTAT': 80285, 'GCTAC': 157588, 'GCTAG': 48590, 'GCTTA': 44208, 'GCTTT': 139645, 'GCTTC': 251447, 'GCTTG': 116179, 'GCTCA': 230263, 'GCTCT': 198326, 'GCTCC': 333536, 'GCTCG': 102895, 'GCTGA': 319308, 'GCTGT': 255763, 'GCTGC': 559307, 'GCTGG': 585766, 'GCCAA': 271563, 'GCCAT': 284452, 'GCCAC': 354424, 'GCCAG': 423768, 'GCCTA': 116790, 'GCCTT': 235884, 'GCCTC': 395356, 'GCCTG': 501406, 'GCCCA': 409504, 'GCCCT': 415286, 'GCCCC': 619276, 'GCCCG': 355623, 'GCCGA': 203186, 'GCCGT': 139955, 'GCCGC': 422254, 'GCCGG': 318680, 'GCGAA': 52642, 'GCGAT': 54404, 'GCGAC': 110901, 'GCGAG': 198627, 'GCGTA': 21676, 'GCGTT': 45787, 'GCGTC': 124866, 'GCGTG': 177959, 'GCGCA': 163118, 'GCGCT': 179020, 'GCGCC': 308095, 'GCGCG': 166856, 'GCGGA': 210106, 'GCGGT': 91380, 'GCGGC': 445290, 'GCGGG': 284560, 'GGAAA': 183122, 'GGAAT': 104671, 'GGAAC': 163073, 'GGAAG': 364276, 'GGATA': 55697, 'GGATT': 102923, 'GGATC': 127428, 'GGATG': 247519, 'GGACA': 299135, 'GGACT': 205104, 'GGACC': 360760, 'GGACG': 206203, 'GGAGA': 445314, 'GGAGT': 196429, 'GGAGC': 478501, 'GGAGG': 617569, 'GGTAA': 42224, 'GGTAT': 49083, 'GGTAC': 75480, 'GGTAG': 53686, 'GGTTA': 36775, 'GGTTT': 105383, 'GGTTC': 123885, 'GGTTG': 98041, 'GGTCA': 168999, 'GGTCT': 162422, 'GGTCC': 218489, 'GGTCG': 109775, 'GGTGA': 213537, 'GGTGT': 173652, 'GGTGC': 234727, 'GGTGG': 433029, 'GGCAA': 198696, 'GGCAT': 163618, 'GGCAC': 221856, 'GGCAG': 388734, 'GGCTA': 118434, 'GGCTT': 183993, 'GGCTC': 259197, 'GGCTG': 457285, 'GGCCA': 464473, 'GGCCT': 393839, 'GGCCC': 629124, 'GGCCG': 404381, 'GGCGA': 139768, 'GGCGT': 134097, 'GGCGC': 277211, 'GGCGG': 391276, 'GGGAA': 212646, 'GGGAT': 126135, 'GGGAC': 282763, 'GGGAG': 351048, 'GGGTA': 54117, 'GGGTT': 105484, 'GGGTC': 202488, 'GGGTG': 237828, 'GGGCA': 294949, 'GGGCT': 293730, 'GGGCC': 554849, 'GGGCG': 264173, 'GGGGA': 286576, 'GGGGT': 166583, 'GGGGC': 421619, 'GGGGG': 320758}, 'overrepresented_sequences': {}} {'total_reads': 2788791, 'total_bases': 161608552, 'q20_bases': 148478196, 'q30_bases': 137834769, 'total_cycles': 58, 'quality_curves': {'A': [37.4033, 37.3922, 37.3815, 37.4058, 37.3862, 37.3999, 37.3671, 37.3457, 37.3478, 37.314, 37.2552, 37.214, 37.159, 37.1089, 37.0355, 36.9511, 36.9146, 36.7815, 36.7379, 36.6635, 36.6322, 36.5431, 36.5163, 36.2332, 36.0367, 35.7302, 35.5324, 35.1926, 34.8972, 34.6864, 34.5202, 34.3428, 34.217, 34.2588, 33.6093, 33.5305, 33.2562, 32.8629, 32.791, 32.6444, 32.1762, 31.9337, 31.8561, 31.2831, 31.0201, 30.712, 30.2996, 30.036, 29.9082, 29.1692, 28.7263, 28.4958, 27.8772, 27.4541, 26.9219, 26.0573, 25.6904, 25.4212], 'T': [37.6261, 37.5906, 37.6021, 37.5853, 37.5266, 37.5189, 37.4705, 37.4497, 37.4276, 37.3935, 37.3486, 37.2877, 37.2845, 37.2546, 37.2498, 37.243, 37.1975, 37.0418, 36.9423, 36.8816, 36.863, 36.762, 36.7879, 36.6031, 36.4216, 36.141, 35.933, 35.639, 35.4084, 35.1725, 35.0721, 34.907, 34.7738, 34.7238, 34.2301, 34.0747, 33.8034, 33.5169, 33.3294, 33.2024, 32.8575, 32.5637, 32.4407, 31.988, 31.5935, 31.425, 30.9926, 30.7038, 30.5292, 29.9833, 29.3896, 29.1461, 28.5928, 28.1015, 27.5604, 26.8723, 26.3504, 26.0176], 'C': [37.6076, 37.6267, 37.5844, 37.583, 37.5888, 37.5671, 37.5688, 37.5543, 37.523, 37.5299, 37.5026, 37.4731, 37.4332, 37.4388, 37.3638, 37.2944, 37.3087, 37.258, 37.182, 37.0844, 37.007, 36.9969, 36.9365, 36.7485, 36.5261, 36.3484, 36.1497, 35.8883, 35.6848, 35.4372, 35.3441, 35.2227, 35.0456, 35.0545, 34.6221, 34.5012, 34.2127, 33.8995, 33.7869, 33.656, 33.2469, 32.9969, 32.8326, 32.3864, 32.1234, 31.6787, 31.3772, 31.0609, 30.757, 30.1115, 29.8203, 29.3418, 28.7503, 28.362, 27.7602, 26.9515, 26.5174, 25.8689], 'G': [37.4632, 37.4591, 37.4249, 37.4368, 37.4254, 37.4076, 37.391, 37.3919, 37.3712, 37.3632, 37.3432, 37.2885, 37.2453, 37.2119, 37.1283, 37.0501, 37.0478, 36.9772, 36.9256, 36.8461, 36.7778, 36.6843, 36.6591, 36.4313, 36.2791, 36.0637, 35.8782, 35.6325, 35.4055, 35.1661, 35.0632, 34.91, 34.7845, 34.7693, 34.3637, 34.2209, 33.9192, 33.6096, 33.515, 33.3688, 32.8854, 32.7554, 32.5181, 32.1173, 31.7786, 31.3641, 30.9273, 30.6453, 30.3833, 29.7423, 29.3489, 28.9627, 28.3083, 27.8747, 27.241, 26.4982, 26.0975, 25.4812], 'mean': [37.5287, 37.5218, 37.5, 37.5033, 37.485, 37.4742, 37.4522, 37.4408, 37.4204, 37.4058, 37.3709, 37.3233, 37.2877, 37.2639, 37.2014, 37.1403, 37.1271, 37.0291, 36.9626, 36.8845, 36.8305, 36.7593, 36.7374, 36.5174, 36.3324, 36.0968, 35.8963, 35.6189, 35.3858, 35.1462, 35.0365, 34.887, 34.7403, 34.7375, 34.2601, 34.127, 33.8449, 33.5257, 33.4032, 33.2692, 32.8436, 32.6148, 32.4587, 32.0047, 31.6836, 31.3369, 30.949, 30.6539, 30.4288, 29.7888, 29.3688, 29.0204, 28.4135, 27.9793, 27.3966, 26.624, 26.1919, 25.6971]}, 'content_curves': {'A': [0.200947, 0.193982, 0.202293, 0.202526, 0.199245, 0.204589, 0.201073, 0.19683, 0.204711, 0.198647, 0.20135, 0.206163, 0.203726, 0.198851, 0.206033, 0.202282, 0.199005, 0.204892, 0.201394, 0.197781, 0.203847, 0.200546, 0.19724, 0.202301, 0.197597, 0.196109, 0.201634, 0.196139, 0.195116, 0.200329, 0.196918, 0.195275, 0.201262, 0.197981, 0.194732, 0.201076, 0.197382, 0.195229, 0.203164, 0.197539, 0.195686, 0.200534, 0.197103, 0.193269, 0.1995, 0.198386, 0.19372, 0.19989, 0.19419, 0.194791, 0.197417, 0.194629, 0.194887, 0.199098, 0.197543, 0.194315, 0.198141, 0.194634], 'T': [0.245373, 0.22922, 0.235917, 0.228597, 0.223413, 0.230989, 0.226878, 0.225735, 0.231438, 0.232003, 0.22264, 0.228739, 0.229975, 0.219904, 0.225482, 0.225142, 0.219732, 0.225145, 0.226207, 0.222206, 0.224816, 0.226575, 0.220751, 0.22473, 0.22433, 0.220366, 0.225241, 0.225653, 0.22157, 0.22598, 0.225918, 0.220426, 0.226935, 0.227255, 0.224073, 0.22674, 0.226037, 0.2217, 0.221871, 0.223729, 0.219393, 0.225009, 0.224834, 0.221577, 0.223732, 0.226459, 0.22036, 0.225397, 0.225969, 0.222056, 0.220836, 0.223831, 0.216074, 0.218938, 0.219676, 0.214769, 0.218812, 0.216933], 'C': [0.259859, 0.273669, 0.263463, 0.265974, 0.274304, 0.266262, 0.269648, 0.276591, 0.269857, 0.272278, 0.27747, 0.272494, 0.271177, 0.278522, 0.275237, 0.273276, 0.279658, 0.27578, 0.276772, 0.279677, 0.275638, 0.27426, 0.281144, 0.276, 0.280373, 0.286258, 0.278144, 0.278609, 0.282414, 0.275936, 0.278282, 0.282744, 0.277447, 0.279143, 0.283751, 0.278514, 0.282053, 0.284265, 0.281209, 0.281258, 0.285118, 0.278698, 0.281398, 0.287039, 0.283799, 0.280966, 0.286491, 0.281994, 0.280322, 0.283331, 0.283935, 0.283507, 0.288983, 0.284641, 0.285881, 0.28917, 0.285099, 0.286952], 'G': [0.293821, 0.303118, 0.298327, 0.302902, 0.303038, 0.298161, 0.302401, 0.300843, 0.293993, 0.297071, 0.298539, 0.292604, 0.295123, 0.302723, 0.293248, 0.2993, 0.301605, 0.294183, 0.295627, 0.300336, 0.295699, 0.298619, 0.300864, 0.296968, 0.297699, 0.297266, 0.294981, 0.299599, 0.3009, 0.297754, 0.298882, 0.301556, 0.294356, 0.295621, 0.297445, 0.293671, 0.294528, 0.298806, 0.293757, 0.297474, 0.299803, 0.29576, 0.296665, 0.298115, 0.292965, 0.294189, 0.299428, 0.292719, 0.299519, 0.299822, 0.297812, 0.298033, 0.300056, 0.297323, 0.296901, 0.301746, 0.297948, 0.30148], 'N': [0, 1.07573e-05, 0, 0, 0, 0, 0, 7.17157e-07, 0, 0, 7.17157e-07, 0, 0, 1.07573e-06, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3.94811e-06, 1.43588e-06, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7.22287e-07], 'GC': [0.55368, 0.576787, 0.56179, 0.568877, 0.577342, 0.564423, 0.572049, 0.577434, 0.563851, 0.569349, 0.576009, 0.565098, 0.5663, 0.581244, 0.568485, 0.572576, 0.581263, 0.569963, 0.572399, 0.580013, 0.571336, 0.572879, 0.582009, 0.572969, 0.578073, 0.583524, 0.573124, 0.578208, 0.583314, 0.573691, 0.577164, 0.584299, 0.571803, 0.574764, 0.581195, 0.572184, 0.576581, 0.583071, 0.574966, 0.578732, 0.584921, 0.574457, 0.578063, 0.585154, 0.576764, 0.575154, 0.58592, 0.574713, 0.579841, 0.583152, 0.581747, 0.58154, 0.589039, 0.581965, 0.582782, 0.590916, 0.583047, 0.588432]}, 'kmer_count': {'AAAAA': 276975, 'AAAAT': 147479, 'AAAAC': 112353, 'AAAAG': 144276, 'AAATA': 125828, 'AAATT': 100504, 'AAATC': 91651, 'AAATG': 108189, 'AAACA': 136433, 'AAACT': 120079, 'AAACC': 102343, 'AAACG': 40281, 'AAAGA': 126844, 'AAAGT': 114505, 'AAAGC': 130408, 'AAAGG': 145922, 'AATAA': 99512, 'AATAT': 60367, 'AATAC': 51324, 'AATAG': 55524, 'AATTA': 56214, 'AATTT': 103885, 'AATTC': 83689, 'AATTG': 56577, 'AATCA': 75902, 'AATCT': 112201, 'AATCC': 91656, 'AATCG': 31509, 'AATGA': 90355, 'AATGT': 89826, 'AATGC': 88893, 'AATGG': 127721, 'AACAA': 96775, 'AACAT': 91691, 'AACAC': 104134, 'AACAG': 130847, 'AACTA': 33776, 'AACTT': 142347, 'AACTC': 122124, 'AACTG': 129483, 'AACCA': 92118, 'AACCT': 87980, 'AACCC': 99316, 'AACCG': 36337, 'AACGA': 21334, 'AACGT': 28989, 'AACGC': 36973, 'AACGG': 47497, 'AAGAA': 145502, 'AAGAT': 108151, 'AAGAC': 90115, 'AAGAG': 151260, 'AAGTA': 73968, 'AAGTT': 107693, 'AAGTC': 118061, 'AAGTG': 132501, 'AAGCA': 115866, 'AAGCT': 125553, 'AAGCC': 177016, 'AAGCG': 85748, 'AAGGA': 156353, 'AAGGT': 125281, 'AAGGC': 208666, 'AAGGG': 203629, 'ATAAA': 107935, 'ATAAT': 48927, 'ATAAC': 36729, 'ATAAG': 50240, 'ATATA': 46292, 'ATATT': 58799, 'ATATC': 39334, 'ATATG': 40465, 'ATACA': 74098, 'ATACT': 68231, 'ATACC': 40943, 'ATACG': 16069, 'ATAGA': 62060, 'ATAGT': 57151, 'ATAGC': 67093, 'ATAGG': 72112, 'ATTAA': 54048, 'ATTAT': 36854, 'ATTAC': 45947, 'ATTAG': 34517, 'ATTTA': 53362, 'ATTTT': 126184, 'ATTTC': 108485, 'ATTTG': 106499, 'ATTCA': 90209, 'ATTCT': 111059, 'ATTCC': 96652, 'ATTCG': 34524, 'ATTGA': 68230, 'ATTGT': 57986, 'ATTGC': 63233, 'ATTGG': 84987, 'ATCAA': 72778, 'ATCAT': 131202, 'ATCAC': 95731, 'ATCAG': 136541, 'ATCTA': 31685, 'ATCTT': 229645, 'ATCTC': 181297, 'ATCTG': 178246, 'ATCCA': 149908, 'ATCCT': 139928, 'ATCCC': 100829, 'ATCCG': 62310, 'ATCGA': 29753, 'ATCGT': 40825, 'ATCGC': 40538, 'ATCGG': 43478, 'ATGAA': 131788, 'ATGAT': 137714, 'ATGAC': 99375, 'ATGAG': 162462, 'ATGTA': 81640, 'ATGTT': 152860, 'ATGTC': 149113, 'ATGTG': 104098, 'ATGCA': 86765, 'ATGCT': 106525, 'ATGCC': 149550, 'ATGCG': 96034, 'ATGGA': 127617, 'ATGGT': 162468, 'ATGGC': 213569, 'ATGGG': 180874, 'ACAAA': 145976, 'ACAAT': 84118, 'ACAAC': 65060, 'ACAAG': 100912, 'ACATA': 67943, 'ACATT': 102111, 'ACATC': 115194, 'ACATG': 118815, 'ACACA': 153640, 'ACACT': 90414, 'ACACC': 147984, 'ACACG': 85596, 'ACAGA': 148552, 'ACAGT': 108025, 'ACAGC': 219000, 'ACAGG': 228572, 'ACTAA': 32691, 'ACTAT': 30801, 'ACTAC': 26261, 'ACTAG': 31445, 'ACTTA': 32991, 'ACTTT': 115413, 'ACTTC': 165939, 'ACTTG': 181799, 'ACTCA': 109991, 'ACTCT': 104489, 'ACTCC': 153150, 'ACTCG': 100345, 'ACTGA': 103146, 'ACTGT': 114880, 'ACTGC': 163641, 'ACTGG': 195700, 'ACCAA': 76105, 'ACCAT': 112101, 'ACCAC': 196677, 'ACCAG': 243460, 'ACCTA': 27657, 'ACCTT': 146388, 'ACCTC': 184187, 'ACCTG': 185683, 'ACCCA': 149727, 'ACCCT': 123007, 'ACCCC': 143840, 'ACCCG': 82117, 'ACCGA': 50631, 'ACCGT': 53548, 'ACCGC': 70294, 'ACCGG': 64304, 'ACGAA': 47029, 'ACGAT': 51603, 'ACGAC': 44785, 'ACGAG': 60760, 'ACGTA': 31197, 'ACGTT': 69310, 'ACGTC': 81965, 'ACGTG': 77800, 'ACGCA': 61781, 'ACGCT': 64960, 'ACGCC': 102587, 'ACGCG': 47634, 'ACGGA': 69344, 'ACGGT': 78377, 'ACGGC': 106010, 'ACGGG': 109380, 'AGAAA': 164598, 'AGAAT': 83987, 'AGAAC': 124020, 'AGAAG': 234414, 'AGATA': 69587, 'AGATT': 65417, 'AGATC': 100671, 'AGATG': 191578, 'AGACA': 120533, 'AGACT': 80225, 'AGACC': 136458, 'AGACG': 83917, 'AGAGA': 168296, 'AGAGT': 93853, 'AGAGC': 165622, 'AGAGG': 275524, 'AGTAA': 62901, 'AGTAT': 46310, 'AGTAC': 60604, 'AGTAG': 88380, 'AGTTA': 47250, 'AGTTT': 115511, 'AGTTC': 117744, 'AGTTG': 120116, 'AGTCA': 110542, 'AGTCT': 118635, 'AGTCC': 171475, 'AGTCG': 71859, 'AGTGA': 128466, 'AGTGT': 99474, 'AGTGC': 128207, 'AGTGG': 195060, 'AGCAA': 102010, 'AGCAT': 117740, 'AGCAC': 187796, 'AGCAG': 330009, 'AGCTA': 49433, 'AGCTT': 201139, 'AGCTC': 250664, 'AGCTG': 332182, 'AGCCA': 245660, 'AGCCT': 203716, 'AGCCC': 269315, 'AGCCG': 168998, 'AGCGA': 68909, 'AGCGT': 102328, 'AGCGC': 140229, 'AGCGG': 172778, 'AGGAA': 253476, 'AGGAT': 165255, 'AGGAC': 184353, 'AGGAG': 326992, 'AGGTA': 115282, 'AGGTT': 133767, 'AGGTC': 209838, 'AGGTG': 289880, 'AGGCA': 253119, 'AGGCT': 264711, 'AGGCC': 326298, 'AGGCG': 204286, 'AGGGA': 234084, 'AGGGT': 198216, 'AGGGC': 358490, 'AGGGG': 399293, 'TAAAA': 123081, 'TAAAT': 73884, 'TAAAC': 51562, 'TAAAG': 58424, 'TAATA': 38795, 'TAATT': 56455, 'TAATC': 51998, 'TAATG': 56473, 'TAACA': 56838, 'TAACT': 54656, 'TAACC': 35326, 'TAACG': 10251, 'TAAGA': 53579, 'TAAGT': 36458, 'TAAGC': 43874, 'TAAGG': 65982, 'TATAA': 38268, 'TATAT': 37522, 'TATAC': 26220, 'TATAG': 36778, 'TATTA': 29614, 'TATTT': 100552, 'TATTC': 54770, 'TATTG': 58841, 'TATCA': 48521, 'TATCT': 54872, 'TATCC': 48717, 'TATCG': 18846, 'TATGA': 40445, 'TATGT': 39169, 'TATGC': 29404, 'TATGG': 59495, 'TACAA': 68529, 'TACAT': 63318, 'TACAC': 66219, 'TACAG': 100185, 'TACTA': 18253, 'TACTT': 99839, 'TACTC': 73619, 'TACTG': 76706, 'TACCA': 56006, 'TACCT': 45135, 'TACCC': 50385, 'TACCG': 26327, 'TACGA': 15350, 'TACGT': 21999, 'TACGC': 16905, 'TACGG': 35085, 'TAGAA': 80734, 'TAGAT': 63512, 'TAGAC': 49762, 'TAGAG': 84593, 'TAGTA': 48960, 'TAGTT': 58045, 'TAGTC': 62663, 'TAGTG': 67466, 'TAGCA': 60782, 'TAGCT': 70330, 'TAGCC': 92426, 'TAGCG': 54003, 'TAGGA': 89793, 'TAGGT': 79895, 'TAGGC': 88160, 'TAGGG': 106401, 'TTAAA': 96472, 'TTAAT': 65731, 'TTAAC': 44117, 'TTAAG': 56867, 'TTATA': 38802, 'TTATT': 70427, 'TTATC': 47246, 'TTATG': 43516, 'TTACA': 72165, 'TTACT': 40293, 'TTACC': 35699, 'TTACG': 22311, 'TTAGA': 32507, 'TTAGT': 45365, 'TTAGC': 43388, 'TTAGG': 61512, 'TTTAA': 95741, 'TTTAT': 82981, 'TTTAC': 54839, 'TTTAG': 51956, 'TTTTA': 94414, 'TTTTT': 285729, 'TTTTC': 182247, 'TTTTG': 136471, 'TTTCA': 133654, 'TTTCT': 258905, 'TTTCC': 174896, 'TTTCG': 32982, 'TTTGA': 110601, 'TTTGT': 158203, 'TTTGC': 128163, 'TTTGG': 154580, 'TTCAA': 123079, 'TTCAT': 163459, 'TTCAC': 187847, 'TTCAG': 229047, 'TTCTA': 60513, 'TTCTT': 382491, 'TTCTC': 333738, 'TTCTG': 258143, 'TTCCA': 180777, 'TTCCT': 237404, 'TTCCC': 193448, 'TTCCG': 112470, 'TTCGA': 41677, 'TTCGT': 32640, 'TTCGC': 35252, 'TTCGG': 58546, 'TTGAA': 151115, 'TTGAT': 167297, 'TTGAC': 123850, 'TTGAG': 181799, 'TTGTA': 123629, 'TTGTT': 168088, 'TTGTC': 193820, 'TTGTG': 133411, 'TTGCA': 120416, 'TTGCT': 159079, 'TTGCC': 170648, 'TTGCG': 107874, 'TTGGA': 172211, 'TTGGT': 191869, 'TTGGC': 247413, 'TTGGG': 238193, 'TCAAA': 143012, 'TCAAT': 113623, 'TCAAC': 65207, 'TCAAG': 98085, 'TCATA': 85259, 'TCATT': 99827, 'TCATC': 256443, 'TCATG': 174542, 'TCACA': 166887, 'TCACT': 151251, 'TCACC': 186480, 'TCACG': 90537, 'TCAGA': 135791, 'TCAGT': 130544, 'TCAGC': 272091, 'TCAGG': 276829, 'TCTAA': 40536, 'TCTAT': 46580, 'TCTAC': 52726, 'TCTAG': 54561, 'TCTTA': 64702, 'TCTTT': 202660, 'TCTTC': 407058, 'TCTTG': 380096, 'TCTCA': 147908, 'TCTCT': 223453, 'TCTCC': 381568, 'TCTCG': 164452, 'TCTGA': 147601, 'TCTGT': 173159, 'TCTGC': 262686, 'TCTGG': 352443, 'TCCAA': 125946, 'TCCAT': 164936, 'TCCAC': 292153, 'TCCAG': 449448, 'TCCTA': 51751, 'TCCTT': 299321, 'TCCTC': 498872, 'TCCTG': 308766, 'TCCCA': 225067, 'TCCCT': 158087, 'TCCCC': 239960, 'TCCCG': 188248, 'TCCGA': 70194, 'TCCGT': 104056, 'TCCGC': 138445, 'TCCGG': 172392, 'TCGAA': 88523, 'TCGAT': 80700, 'TCGAC': 33224, 'TCGAG': 52317, 'TCGTA': 68100, 'TCGTT': 62603, 'TCGTC': 126124, 'TCGTG': 84221, 'TCGCA': 67347, 'TCGCT': 85044, 'TCGCC': 106318, 'TCGCG': 59077, 'TCGGA': 74739, 'TCGGT': 76026, 'TCGGC': 151173, 'TCGGG': 168422, 'TGAAA': 104920, 'TGAAT': 84443, 'TGAAC': 118587, 'TGAAG': 253686, 'TGATA': 52401, 'TGATT': 57990, 'TGATC': 135897, 'TGATG': 260639, 'TGACA': 125267, 'TGACT': 95597, 'TGACC': 155826, 'TGACG': 114569, 'TGAGA': 152652, 'TGAGT': 108178, 'TGAGC': 221776, 'TGAGG': 297621, 'TGTAA': 71223, 'TGTAT': 58493, 'TGTAC': 89982, 'TGTAG': 164090, 'TGTTA': 58748, 'TGTTT': 137092, 'TGTTC': 160293, 'TGTTG': 225503, 'TGTCA': 157061, 'TGTCT': 177249, 'TGTCC': 268636, 'TGTCG': 104395, 'TGTGA': 117759, 'TGTGT': 140906, 'TGTGC': 150709, 'TGTGG': 244526, 'TGCAA': 97123, 'TGCAT': 117526, 'TGCAC': 157520, 'TGCAG': 303121, 'TGCTA': 43760, 'TGCTT': 184114, 'TGCTC': 238224, 'TGCTG': 372193, 'TGCCA': 208882, 'TGCCT': 196825, 'TGCCC': 265388, 'TGCCG': 181236, 'TGCGA': 43454, 'TGCGT': 70755, 'TGCGC': 125449, 'TGCGG': 204239, 'TGGAA': 201098, 'TGGAT': 143716, 'TGGAC': 164259, 'TGGAG': 327533, 'TGGTA': 103772, 'TGGTT': 153040, 'TGGTC': 257651, 'TGGTG': 321279, 'TGGCA': 257489, 'TGGCT': 275245, 'TGGCC': 399769, 'TGGCG': 201321, 'TGGGA': 245163, 'TGGGT': 237935, 'TGGGC': 354873, 'TGGGG': 507318, 'CAAAA': 154887, 'CAAAT': 93529, 'CAAAC': 139787, 'CAAAG': 178380, 'CAATA': 51188, 'CAATT': 64199, 'CAATC': 82163, 'CAATG': 136425, 'CAACA': 105442, 'CAACT': 79394, 'CAACC': 80396, 'CAACG': 40951, 'CAAGA': 96721, 'CAAGT': 100098, 'CAAGC': 97397, 'CAAGG': 168036, 'CATAA': 58948, 'CATAT': 45514, 'CATAC': 78153, 'CATAG': 113330, 'CATTA': 54609, 'CATTT': 104913, 'CATTC': 125497, 'CATTG': 106379, 'CATCA': 192473, 'CATCT': 238664, 'CATCC': 204421, 'CATCG': 73796, 'CATGA': 171611, 'CATGT': 149471, 'CATGC': 142647, 'CATGG': 227004, 'CACAA': 145634, 'CACAT': 149173, 'CACAC': 186598, 'CACAG': 284130, 'CACTA': 41257, 'CACTT': 134677, 'CACTC': 147477, 'CACTG': 259943, 'CACCA': 310150, 'CACCT': 267244, 'CACCC': 203460, 'CACCG': 106977, 'CACGA': 99670, 'CACGT': 130845, 'CACGC': 137397, 'CACGG': 168652, 'CAGAA': 195978, 'CAGAT': 139177, 'CAGAC': 147518, 'CAGAG': 257808, 'CAGTA': 79355, 'CAGTT': 134936, 'CAGTC': 151758, 'CAGTG': 211624, 'CAGCA': 385654, 'CAGCT': 413403, 'CAGCC': 387509, 'CAGCG': 221080, 'CAGGA': 384156, 'CAGGT': 324550, 'CAGGC': 425316, 'CAGGG': 516201, 'CTAAA': 44689, 'CTAAT': 29004, 'CTAAC': 34718, 'CTAAG': 44742, 'CTATA': 20045, 'CTATT': 46934, 'CTATC': 38650, 'CTATG': 39293, 'CTACA': 49779, 'CTACT': 43476, 'CTACC': 41297, 'CTACG': 21585, 'CTAGA': 54838, 'CTAGT': 21166, 'CTAGC': 41655, 'CTAGG': 67252, 'CTTAA': 63099, 'CTTAT': 38083, 'CTTAC': 29921, 'CTTAG': 58613, 'CTTTA': 93834, 'CTTTT': 159658, 'CTTTC': 164463, 'CTTTG': 186458, 'CTTCA': 328030, 'CTTCT': 453343, 'CTTCC': 319616, 'CTTCG': 63033, 'CTTGA': 275770, 'CTTGT': 243424, 'CTTGC': 248800, 'CTTGG': 387727, 'CTCAA': 124654, 'CTCAT': 188524, 'CTCAC': 141256, 'CTCAG': 266901, 'CTCTA': 60302, 'CTCTT': 242201, 'CTCTC': 204298, 'CTCTG': 299438, 'CTCCA': 442180, 'CTCCT': 499776, 'CTCCC': 274160, 'CTCCG': 194409, 'CTCGA': 111543, 'CTCGT': 165940, 'CTCGC': 160970, 'CTCGG': 232010, 'CTGAA': 140853, 'CTGAT': 90424, 'CTGAC': 135302, 'CTGAG': 238794, 'CTGTA': 100755, 'CTGTT': 143446, 'CTGTC': 215558, 'CTGTG': 248130, 'CTGCA': 311376, 'CTGCT': 364383, 'CTGCC': 346813, 'CTGCG': 144034, 'CTGGA': 306586, 'CTGGT': 288523, 'CTGGC': 362206, 'CTGGG': 555118, 'CCAAA': 158833, 'CCAAT': 65051, 'CCAAC': 98260, 'CCAAG': 153212, 'CCATA': 77958, 'CCATT': 109018, 'CCATC': 188835, 'CCATG': 240519, 'CCACA': 260290, 'CCACT': 181167, 'CCACC': 361374, 'CCACG': 226964, 'CCAGA': 238431, 'CCAGT': 182430, 'CCAGC': 484251, 'CCAGG': 621690, 'CCTAA': 36703, 'CCTAT': 41334, 'CCTAC': 32810, 'CCTAG': 49432, 'CCTTA': 44289, 'CCTTT': 141207, 'CCTTC': 310134, 'CCTTG': 342691, 'CCTCA': 250198, 'CCTCT': 270028, 'CCTCC': 511017, 'CCTCG': 245250, 'CCTGA': 161684, 'CCTGT': 172758, 'CCTGC': 313405, 'CCTGG': 448562, 'CCCAA': 139849, 'CCCAT': 165512, 'CCCAC': 256037, 'CCCAG': 440993, 'CCCTA': 43264, 'CCCTT': 185104, 'CCCTC': 253828, 'CCCTG': 276405, 'CCCCA': 304481, 'CCCCT': 206437, 'CCCCC': 256341, 'CCCCG': 221453, 'CCCGA': 110501, 'CCCGT': 121327, 'CCCGC': 198845, 'CCCGG': 284238, 'CCGAA': 67336, 'CCGAT': 62230, 'CCGAC': 67918, 'CCGAG': 138232, 'CCGTA': 60206, 'CCGTT': 82209, 'CCGTC': 141340, 'CCGTG': 147660, 'CCGCA': 137185, 'CCGCT': 181531, 'CCGCC': 246914, 'CCGCG': 126942, 'CCGGA': 130353, 'CCGGT': 128600, 'CCGGC': 240712, 'CCGGG': 292077, 'CGAAA': 37015, 'CGAAT': 34868, 'CGAAC': 53583, 'CGAAG': 123296, 'CGATA': 16797, 'CGATT': 26609, 'CGATC': 68500, 'CGATG': 149390, 'CGACA': 46764, 'CGACT': 51942, 'CGACC': 62373, 'CGACG': 39020, 'CGAGA': 67608, 'CGAGT': 54669, 'CGAGC': 86034, 'CGAGG': 150005, 'CGTAA': 17851, 'CGTAT': 21528, 'CGTAC': 46470, 'CGTAG': 112178, 'CGTTA': 15334, 'CGTTT': 59642, 'CGTTC': 96234, 'CGTTG': 111287, 'CGTCA': 86033, 'CGTCT': 121277, 'CGTCC': 161134, 'CGTCG': 105070, 'CGTGA': 77860, 'CGTGT': 83373, 'CGTGC': 113987, 'CGTGG': 183021, 'CGCAA': 33518, 'CGCAT': 54751, 'CGCAC': 102883, 'CGCAG': 212410, 'CGCTA': 15559, 'CGCTT': 103501, 'CGCTC': 167416, 'CGCTG': 194033, 'CGCCA': 140800, 'CGCCT': 141533, 'CGCCC': 202965, 'CGCCG': 163421, 'CGCGA': 45445, 'CGCGT': 52160, 'CGCGC': 100666, 'CGCGG': 154376, 'CGGAA': 86456, 'CGGAT': 102331, 'CGGAC': 84266, 'CGGAG': 149591, 'CGGTA': 72931, 'CGGTT': 72385, 'CGGTC': 112023, 'CGGTG': 172273, 'CGGCA': 147358, 'CGGCT': 170145, 'CGGCC': 286037, 'CGGCG': 190422, 'CGGGA': 154235, 'CGGGT': 110925, 'CGGGC': 273896, 'CGGGG': 299913, 'GAAAA': 124239, 'GAAAT': 108713, 'GAAAC': 95780, 'GAAAG': 136716, 'GAATA': 50891, 'GAATT': 78780, 'GAATC': 84290, 'GAATG': 94124, 'GAACA': 124637, 'GAACT': 173830, 'GAACC': 99214, 'GAACG': 43118, 'GAAGA': 218104, 'GAAGT': 180539, 'GAAGC': 232279, 'GAAGG': 315801, 'GATAA': 46972, 'GATAT': 41545, 'GATAC': 43705, 'GATAG': 53021, 'GATTA': 29724, 'GATTT': 84192, 'GATTC': 68409, 'GATTG': 51945, 'GATCA': 118033, 'GATCT': 212015, 'GATCC': 109283, 'GATCG': 30637, 'GATGA': 227368, 'GATGT': 207237, 'GATGC': 177135, 'GATGG': 269839, 'GACAA': 83791, 'GACAT': 100222, 'GACAC': 120763, 'GACAG': 188838, 'GACTA': 27428, 'GACTT': 120076, 'GACTC': 126198, 'GACTG': 110514, 'GACCA': 169334, 'GACCT': 145817, 'GACCC': 149429, 'GACCG': 69284, 'GACGA': 68088, 'GACGT': 78774, 'GACGC': 86111, 'GACGG': 113108, 'GAGAA': 184356, 'GAGAT': 116290, 'GAGAC': 134827, 'GAGAG': 209363, 'GAGTA': 54752, 'GAGTT': 99851, 'GAGTC': 138807, 'GAGTG': 137405, 'GAGCA': 175922, 'GAGCT': 226662, 'GAGCC': 234423, 'GAGCG': 124320, 'GAGGA': 297011, 'GAGGT': 215924, 'GAGGC': 327509, 'GAGGG': 363195, 'GTAAA': 57796, 'GTAAT': 59934, 'GTAAC': 40815, 'GTAAG': 47578, 'GTATA': 32815, 'GTATT': 64651, 'GTATC': 44647, 'GTATG': 44563, 'GTACA': 101888, 'GTACT': 116404, 'GTACC': 60297, 'GTACG': 30029, 'GTAGA': 129618, 'GTAGT': 112478, 'GTAGC': 124899, 'GTAGG': 162414, 'GTTAA': 49014, 'GTTAT': 40354, 'GTTAC': 39584, 'GTTAG': 36173, 'GTTTA': 42377, 'GTTTT': 126495, 'GTTTC': 144203, 'GTTTG': 120896, 'GTTCA': 150017, 'GTTCT': 208389, 'GTTCC': 133513, 'GTTCG': 38209, 'GTTGA': 167018, 'GTTGT': 157220, 'GTTGC': 116474, 'GTTGG': 221326, 'GTCAA': 98762, 'GTCAT': 130965, 'GTCAC': 168162, 'GTCAG': 180437, 'GTCTA': 40828, 'GTCTT': 196985, 'GTCTC': 196239, 'GTCTG': 197410, 'GTCCA': 258032, 'GTCCT': 279068, 'GTCCC': 244840, 'GTCCG': 115621, 'GTCGA': 71535, 'GTCGT': 101206, 'GTCGC': 81137, 'GTCGG': 136429, 'GTGAA': 136365, 'GTGAT': 108944, 'GTGAC': 131529, 'GTGAG': 195793, 'GTGTA': 76078, 'GTGTT': 115571, 'GTGTC': 145794, 'GTGTG': 163725, 'GTGCA': 156058, 'GTGCT': 207240, 'GTGCC': 186644, 'GTGCG': 94920, 'GTGGA': 227490, 'GTGGT': 190076, 'GTGGC': 310558, 'GTGGG': 371038, 'GCAAA': 120150, 'GCAAT': 71910, 'GCAAC': 78707, 'GCAAG': 109628, 'GCATA': 66470, 'GCATT': 80225, 'GCATC': 149876, 'GCATG': 155650, 'GCACA': 185484, 'GCACT': 160518, 'GCACC': 194829, 'GCACG': 134144, 'GCAGA': 218006, 'GCAGT': 156202, 'GCAGC': 437289, 'GCAGG': 524181, 'GCTAA': 43143, 'GCTAT': 26195, 'GCTAC': 44774, 'GCTAG': 49747, 'GCTTA': 48532, 'GCTTT': 143783, 'GCTTC': 282524, 'GCTTG': 250091, 'GCTCA': 212331, 'GCTCT': 208766, 'GCTCC': 368567, 'GCTCG': 162393, 'GCTGA': 193630, 'GCTGT': 245704, 'GCTGC': 428455, 'GCTGG': 517339, 'GCCAA': 135388, 'GCCAT': 173519, 'GCCAC': 286470, 'GCCAG': 393348, 'GCCTA': 37920, 'GCCTT': 210202, 'GCCTC': 338352, 'GCCTG': 325706, 'GCCCA': 327076, 'GCCCT': 274320, 'GCCCC': 358356, 'GCCCG': 228194, 'GCCGA': 105811, 'GCCGT': 152568, 'GCCGC': 286544, 'GCCGG': 273329, 'GCGAA': 46633, 'GCGAT': 66533, 'GCGAC': 54754, 'GCGAG': 107731, 'GCGTA': 38426, 'GCGTT': 67522, 'GCGTC': 125263, 'GCGTG': 148224, 'GCGCA': 136983, 'GCGCT': 150580, 'GCGCC': 195807, 'GCGCG': 120860, 'GCGGA': 148635, 'GCGGT': 148139, 'GCGGC': 299226, 'GCGGG': 272795, 'GGAAA': 158408, 'GGAAT': 105154, 'GGAAC': 145404, 'GGAAG': 336894, 'GGATA': 46685, 'GGATT': 82781, 'GGATC': 164014, 'GGATG': 278616, 'GGACA': 199261, 'GGACT': 157886, 'GGACC': 180826, 'GGACG': 108734, 'GGAGA': 256545, 'GGAGT': 173677, 'GGAGC': 288966, 'GGAGG': 479334, 'GGTAA': 54035, 'GGTAT': 59000, 'GGTAC': 111289, 'GGTAG': 164583, 'GGTTA': 42990, 'GGTTT': 121555, 'GGTTC': 157515, 'GGTTG': 203584, 'GGTCA': 222548, 'GGTCT': 214360, 'GGTCC': 294906, 'GGTCG': 107644, 'GGTGA': 245994, 'GGTGT': 173775, 'GGTGC': 250688, 'GGTGG': 473588, 'GGCAA': 147621, 'GGCAT': 161677, 'GGCAC': 228972, 'GGCAG': 492417, 'GGCTA': 55580, 'GGCTT': 238348, 'GGCTC': 296202, 'GGCTG': 483214, 'GGCCA': 397731, 'GGCCT': 372784, 'GGCCC': 454529, 'GGCCG': 308484, 'GGCGA': 117521, 'GGCGT': 154553, 'GGCGC': 237945, 'GGCGG': 339257, 'GGGAA': 205296, 'GGGAT': 159092, 'GGGAC': 214566, 'GGGAG': 394793, 'GGGTA': 96635, 'GGGTT': 165571, 'GGGTC': 261591, 'GGGTG': 353697, 'GGGCA': 371588, 'GGGCT': 363399, 'GGGCC': 526936, 'GGGCG': 254458, 'GGGGA': 339368, 'GGGGT': 329679, 'GGGGC': 532117, 'GGGGG': 500726}, 'overrepresented_sequences': {}} {'total_reads': 2965080, 'total_bases': 186782780, 'q20_bases': 162975426, 'q30_bases': 150018843, 'total_cycles': 63, 'quality_curves': {'A': [37.2798, 37.2633, 37.2489, 37.2761, 37.2487, 37.2612, 37.2199, 37.1967, 37.1924, 37.153, 37.077, 37.0197, 36.9447, 36.8893, 36.7928, 36.6942, 36.6491, 36.4939, 36.4411, 36.3399, 36.2965, 36.1918, 36.1549, 35.8517, 35.6335, 35.2915, 35.071, 34.6964, 34.3739, 34.1183, 33.9218, 33.7039, 33.5366, 33.5365, 32.8193, 32.6923, 32.386, 31.9753, 31.8974, 31.7343, 31.2785, 31.0189, 30.9361, 30.3729, 30.1014, 29.8068, 29.3726, 29.1112, 28.9896, 28.2656, 27.8236, 27.6053, 26.995, 26.5719, 26.0431, 25.1948, 24.8385, 24.5711, 23.4265, 22.3091, 21.5661, 19.548, 10.2807], 'T': [37.5294, 37.4854, 37.4935, 37.4716, 37.4036, 37.3838, 37.3276, 37.2955, 37.2647, 37.2194, 37.1616, 37.0902, 37.0818, 37.0486, 37.031, 37.0153, 36.962, 36.7812, 36.6822, 36.6135, 36.5769, 36.4571, 36.4573, 36.2238, 36.0195, 35.6944, 35.4479, 35.1248, 34.8549, 34.5989, 34.4735, 34.2543, 34.0879, 34.0021, 33.4548, 33.2415, 32.9658, 32.6372, 32.3993, 32.2818, 31.9202, 31.6142, 31.485, 31.0264, 30.6218, 30.4615, 30.054, 29.7487, 29.5919, 29.046, 28.461, 28.2294, 27.687, 27.2052, 26.6768, 26.0034, 25.4963, 25.1666, 24.2564, 22.9461, 22.1042, 20.3486, 11.3539], 'C': [37.4965, 37.5137, 37.4665, 37.4629, 37.463, 37.4367, 37.4374, 37.4203, 37.3823, 37.3831, 37.3461, 37.3036, 37.2501, 37.2451, 37.1613, 37.0771, 37.0908, 37.0201, 36.9359, 36.8176, 36.7326, 36.6929, 36.6163, 36.4039, 36.158, 35.9453, 35.7199, 35.4125, 35.1702, 34.88, 34.7477, 34.585, 34.3511, 34.3112, 33.7919, 33.6232, 33.2796, 32.939, 32.8093, 32.669, 32.2522, 31.9839, 31.8004, 31.3689, 31.1092, 30.6374, 30.3381, 30.0436, 29.7112, 29.0686, 28.7778, 28.2975, 27.7187, 27.3179, 26.7235, 25.9322, 25.4946, 24.8541, 23.9917, 22.9293, 21.7932, 19.7673, 11.2317], 'G': [37.3199, 37.313, 37.2702, 37.2844, 37.2631, 37.2407, 37.2206, 37.2161, 37.189, 37.177, 37.1448, 37.069, 37.01, 36.9702, 36.8643, 36.7759, 36.7676, 36.6826, 36.6143, 36.5225, 36.4361, 36.3194, 36.2785, 36.0295, 35.861, 35.6045, 35.3909, 35.1018, 34.8369, 34.5692, 34.4099, 34.2146, 34.0223, 33.9491, 33.4696, 33.2758, 32.9139, 32.5826, 32.4707, 32.3051, 31.8149, 31.6826, 31.4511, 31.0333, 30.6902, 30.2785, 29.8382, 29.5419, 29.283, 28.6497, 28.2595, 27.8567, 27.2108, 26.793, 26.1761, 25.4454, 25.0406, 24.4431, 23.51, 22.3834, 21.2435, 19.366, 11.4955], 'mean': [37.4092, 37.3975, 37.3703, 37.373, 37.3465, 37.3302, 37.3032, 37.2867, 37.2594, 37.2382, 37.1908, 37.1277, 37.0784, 37.048, 36.9689, 36.8956, 36.8771, 36.7593, 36.6839, 36.5892, 36.521, 36.4275, 36.3885, 36.1406, 35.935, 35.6607, 35.431, 35.1145, 34.8452, 34.5717, 34.4226, 34.2288, 34.031, 33.9809, 33.4318, 33.248, 32.925, 32.5781, 32.4343, 32.29, 31.8582, 31.6189, 31.4556, 31.0008, 30.6761, 30.3276, 29.9392, 29.6442, 29.4157, 28.7817, 28.3654, 28.0161, 27.4182, 26.9882, 26.4161, 25.6569, 25.2297, 24.7421, 23.7912, 22.6447, 21.6494, 19.7234, 11.1787]}, 'content_curves': {'A': [0.200008, 0.193193, 0.201603, 0.201722, 0.198337, 0.203759, 0.200205, 0.195938, 0.203784, 0.197782, 0.20052, 0.205393, 0.202928, 0.198072, 0.205381, 0.201476, 0.19835, 0.204343, 0.200669, 0.197209, 0.203242, 0.199852, 0.19659, 0.201499, 0.196834, 0.195309, 0.200713, 0.195199, 0.194218, 0.199578, 0.196111, 0.194449, 0.200387, 0.197116, 0.193886, 0.200209, 0.196581, 0.194286, 0.202169, 0.196746, 0.194774, 0.199585, 0.196236, 0.192398, 0.198626, 0.197384, 0.192806, 0.19891, 0.193279, 0.193808, 0.196567, 0.193602, 0.19388, 0.198394, 0.196329, 0.193656, 0.196832, 0.193646, 0.19129, 0.196793, 0.19278, 0.19043, 0.156462], 'T': [0.245162, 0.229192, 0.235785, 0.228424, 0.223346, 0.230984, 0.226786, 0.225555, 0.231244, 0.231709, 0.222125, 0.228298, 0.229293, 0.219115, 0.224873, 0.224465, 0.218935, 0.224379, 0.22535, 0.221148, 0.223953, 0.225459, 0.219805, 0.224202, 0.223611, 0.21975, 0.224825, 0.225026, 0.220955, 0.225305, 0.225054, 0.219642, 0.226106, 0.226236, 0.223126, 0.225969, 0.225087, 0.220847, 0.221258, 0.222962, 0.218708, 0.224255, 0.224121, 0.220987, 0.223092, 0.225717, 0.219529, 0.22467, 0.225034, 0.221121, 0.219972, 0.222743, 0.215069, 0.217854, 0.21843, 0.213552, 0.217958, 0.215621, 0.211717, 0.213975, 0.2156, 0.210043, 0.170295], 'C': [0.260056, 0.273688, 0.263555, 0.266179, 0.274428, 0.266385, 0.269746, 0.276796, 0.269996, 0.272377, 0.27761, 0.272627, 0.271446, 0.278914, 0.275353, 0.273531, 0.279948, 0.276023, 0.276994, 0.280018, 0.275684, 0.274611, 0.281337, 0.276171, 0.280625, 0.286221, 0.278156, 0.278785, 0.282664, 0.276138, 0.278482, 0.282874, 0.277475, 0.279337, 0.283948, 0.278681, 0.282319, 0.284528, 0.281386, 0.281361, 0.28521, 0.278959, 0.281591, 0.287112, 0.283799, 0.281221, 0.286747, 0.282054, 0.280643, 0.283685, 0.284113, 0.283764, 0.28916, 0.284934, 0.286391, 0.289294, 0.285441, 0.287751, 0.290828, 0.286748, 0.288129, 0.290045, 0.318126], 'G': [0.294773, 0.303917, 0.299057, 0.303675, 0.303888, 0.298872, 0.303263, 0.301711, 0.294976, 0.298133, 0.299744, 0.293682, 0.296332, 0.303898, 0.294393, 0.300528, 0.302766, 0.295255, 0.296988, 0.301626, 0.297121, 0.300078, 0.302269, 0.298128, 0.29893, 0.29872, 0.296307, 0.300989, 0.302163, 0.298979, 0.300352, 0.303035, 0.296032, 0.297312, 0.299039, 0.29514, 0.296014, 0.300339, 0.295187, 0.298931, 0.301308, 0.297202, 0.298037, 0.299495, 0.294457, 0.295678, 0.300918, 0.294365, 0.301033, 0.301385, 0.299348, 0.299891, 0.301891, 0.298806, 0.29885, 0.303497, 0.299769, 0.302981, 0.306122, 0.302435, 0.30348, 0.309482, 0.35315], 'N': [0, 1.01178e-05, 0, 0, 0, 0, 0, 6.74518e-07, 0, 0, 6.74518e-07, 0, 0, 1.01178e-06, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1.48403e-05, 8.09476e-06, 2.66454e-05, 1.34914e-06, 0, 0, 1.0119e-05, 0, 0, 0, 0, 1.18073e-05, 0, 0, 0, 6.7482e-07, 4.35259e-05, 4.82497e-05, 1.14719e-05, 0, 0.00196609], 'GC': [0.55483, 0.577605, 0.562612, 0.569854, 0.578317, 0.565257, 0.573009, 0.578506, 0.564973, 0.57051, 0.577355, 0.56631, 0.567778, 0.582812, 0.569747, 0.574059, 0.582714, 0.571278, 0.573981, 0.581644, 0.572805, 0.574689, 0.583606, 0.574299, 0.579554, 0.584941, 0.574462, 0.579775, 0.584827, 0.575117, 0.578834, 0.585909, 0.573507, 0.576649, 0.582988, 0.573821, 0.578332, 0.584867, 0.576573, 0.580292, 0.586518, 0.57616, 0.579628, 0.586606, 0.578256, 0.576898, 0.587665, 0.57642, 0.581677, 0.58507, 0.583461, 0.583655, 0.591051, 0.58374, 0.585241, 0.592791, 0.58521, 0.590733, 0.59695, 0.589183, 0.591609, 0.599527, 0.671276]}, 'kmer_count': {'AAAAA': 349972, 'AAAAT': 168639, 'AAAAC': 133043, 'AAAAG': 169658, 'AAATA': 143226, 'AAATT': 116223, 'AAATC': 104533, 'AAATG': 122399, 'AAACA': 157729, 'AAACT': 137370, 'AAACC': 120944, 'AAACG': 46795, 'AAAGA': 147320, 'AAAGT': 131283, 'AAAGC': 151237, 'AAAGG': 172845, 'AATAA': 113370, 'AATAT': 67730, 'AATAC': 59515, 'AATAG': 64996, 'AATTA': 63771, 'AATTT': 121046, 'AATTC': 96198, 'AATTG': 64543, 'AATCA': 85095, 'AATCT': 127387, 'AATCC': 105249, 'AATCG': 38120, 'AATGA': 101968, 'AATGT': 101213, 'AATGC': 100959, 'AATGG': 145817, 'AACAA': 111744, 'AACAT': 104093, 'AACAC': 119603, 'AACAG': 151680, 'AACTA': 38677, 'AACTT': 161777, 'AACTC': 139370, 'AACTG': 148925, 'AACCA': 106894, 'AACCT': 101173, 'AACCC': 119534, 'AACCG': 43983, 'AACGA': 24777, 'AACGT': 32842, 'AACGC': 42905, 'AACGG': 56153, 'AAGAA': 167898, 'AAGAT': 122594, 'AAGAC': 105660, 'AAGAG': 174528, 'AAGTA': 84013, 'AAGTT': 125213, 'AAGTC': 135274, 'AAGTG': 149799, 'AAGCA': 132834, 'AAGCT': 143062, 'AAGCC': 204364, 'AAGCG': 100410, 'AAGGA': 179677, 'AAGGT': 145009, 'AAGGC': 244174, 'AAGGG': 247173, 'ATAAA': 123645, 'ATAAT': 56422, 'ATAAC': 42326, 'ATAAG': 56945, 'ATATA': 54501, 'ATATT': 66205, 'ATATC': 44562, 'ATATG': 45705, 'ATACA': 84483, 'ATACT': 78340, 'ATACC': 48604, 'ATACG': 18512, 'ATAGA': 70782, 'ATAGT': 64069, 'ATAGC': 77284, 'ATAGG': 84450, 'ATTAA': 62595, 'ATTAT': 41785, 'ATTAC': 51458, 'ATTAG': 39850, 'ATTTA': 61230, 'ATTTT': 148629, 'ATTTC': 124613, 'ATTTG': 120128, 'ATTCA': 101677, 'ATTCT': 126582, 'ATTCC': 112400, 'ATTCG': 40042, 'ATTGA': 76864, 'ATTGT': 65491, 'ATTGC': 72966, 'ATTGG': 98198, 'ATCAA': 83088, 'ATCAT': 148055, 'ATCAC': 108348, 'ATCAG': 153266, 'ATCTA': 35582, 'ATCTT': 257856, 'ATCTC': 205620, 'ATCTG': 201132, 'ATCCA': 169166, 'ATCCT': 159705, 'ATCCC': 122153, 'ATCCG': 72672, 'ATCGA': 34388, 'ATCGT': 46738, 'ATCGC': 48081, 'ATCGG': 52326, 'ATGAA': 148680, 'ATGAT': 155003, 'ATGAC': 113324, 'ATGAG': 184184, 'ATGTA': 91334, 'ATGTT': 173380, 'ATGTC': 169787, 'ATGTG': 118570, 'ATGCA': 99630, 'ATGCT': 120859, 'ATGCC': 172802, 'ATGCG': 111038, 'ATGGA': 145426, 'ATGGT': 182535, 'ATGGC': 249003, 'ATGGG': 214379, 'ACAAA': 168488, 'ACAAT': 93908, 'ACAAC': 74816, 'ACAAG': 115765, 'ACATA': 77933, 'ACATT': 116066, 'ACATC': 130630, 'ACATG': 137267, 'ACACA': 175818, 'ACACT': 102204, 'ACACC': 171753, 'ACACG': 99750, 'ACAGA': 170987, 'ACAGT': 123490, 'ACAGC': 252230, 'ACAGG': 263965, 'ACTAA': 37435, 'ACTAT': 34726, 'ACTAC': 30678, 'ACTAG': 35910, 'ACTTA': 37910, 'ACTTT': 133410, 'ACTTC': 188412, 'ACTTG': 206620, 'ACTCA': 127203, 'ACTCT': 117758, 'ACTCC': 179967, 'ACTCG': 116839, 'ACTGA': 117144, 'ACTGT': 130035, 'ACTGC': 188069, 'ACTGG': 226236, 'ACCAA': 88493, 'ACCAT': 127609, 'ACCAC': 226207, 'ACCAG': 280170, 'ACCTA': 31267, 'ACCTT': 169259, 'ACCTC': 212409, 'ACCTG': 214884, 'ACCCA': 173957, 'ACCCT': 144803, 'ACCCC': 178495, 'ACCCG': 100989, 'ACCGA': 57624, 'ACCGT': 61742, 'ACCGC': 82039, 'ACCGG': 80206, 'ACGAA': 55979, 'ACGAT': 59410, 'ACGAC': 52363, 'ACGAG': 72916, 'ACGTA': 35399, 'ACGTT': 80280, 'ACGTC': 94494, 'ACGTG': 89188, 'ACGCA': 72764, 'ACGCT': 73839, 'ACGCC': 120782, 'ACGCG': 57659, 'ACGGA': 80597, 'ACGGT': 89792, 'ACGGC': 125001, 'ACGGG': 130841, 'AGAAA': 189177, 'AGAAT': 95310, 'AGAAC': 142886, 'AGAAG': 269247, 'AGATA': 79177, 'AGATT': 75178, 'AGATC': 116308, 'AGATG': 220313, 'AGACA': 138637, 'AGACT': 92196, 'AGACC': 161762, 'AGACG': 99551, 'AGAGA': 194413, 'AGAGT': 106634, 'AGAGC': 192397, 'AGAGG': 320611, 'AGTAA': 71049, 'AGTAT': 52019, 'AGTAC': 69317, 'AGTAG': 101031, 'AGTTA': 54097, 'AGTTT': 131064, 'AGTTC': 136319, 'AGTTG': 138629, 'AGTCA': 123901, 'AGTCT': 134482, 'AGTCC': 198519, 'AGTCG': 84230, 'AGTGA': 144964, 'AGTGT': 111027, 'AGTGC': 146494, 'AGTGG': 227405, 'AGCAA': 118257, 'AGCAT': 134330, 'AGCAC': 217080, 'AGCAG': 382124, 'AGCTA': 57905, 'AGCTT': 230498, 'AGCTC': 290595, 'AGCTG': 382969, 'AGCCA': 282609, 'AGCCT': 233871, 'AGCCC': 319874, 'AGCCG': 199812, 'AGCGA': 79424, 'AGCGT': 115786, 'AGCGC': 165835, 'AGCGG': 206026, 'AGGAA': 289725, 'AGGAT': 187605, 'AGGAC': 213626, 'AGGAG': 380779, 'AGGTA': 132205, 'AGGTT': 154054, 'AGGTC': 241661, 'AGGTG': 334310, 'AGGCA': 292458, 'AGGCT': 305118, 'AGGCC': 387287, 'AGGCG': 241551, 'AGGGA': 272399, 'AGGGT': 232799, 'AGGGC': 421602, 'AGGGG': 487505, 'TAAAA': 145061, 'TAAAT': 84836, 'TAAAC': 58986, 'TAAAG': 67873, 'TAATA': 45042, 'TAATT': 64582, 'TAATC': 59203, 'TAATG': 63580, 'TAACA': 64200, 'TAACT': 61289, 'TAACC': 41493, 'TAACG': 12358, 'TAAGA': 60489, 'TAAGT': 41671, 'TAAGC': 50465, 'TAAGG': 76005, 'TATAA': 44916, 'TATAT': 43718, 'TATAC': 29436, 'TATAG': 41140, 'TATTA': 34387, 'TATTT': 114917, 'TATTC': 61812, 'TATTG': 65808, 'TATCA': 54521, 'TATCT': 60654, 'TATCC': 55594, 'TATCG': 22380, 'TATGA': 45230, 'TATGT': 45386, 'TATGC': 33655, 'TATGG': 67705, 'TACAA': 78126, 'TACAT': 72094, 'TACAC': 75411, 'TACAG': 112886, 'TACTA': 21023, 'TACTT': 114015, 'TACTC': 84328, 'TACTG': 88527, 'TACCA': 63486, 'TACCT': 52146, 'TACCC': 60954, 'TACCG': 30530, 'TACGA': 17995, 'TACGT': 25506, 'TACGC': 19722, 'TACGG': 41526, 'TAGAA': 91634, 'TAGAT': 72688, 'TAGAC': 57805, 'TAGAG': 97022, 'TAGTA': 55234, 'TAGTT': 65826, 'TAGTC': 71972, 'TAGTG': 77662, 'TAGCA': 69342, 'TAGCT': 80468, 'TAGCC': 107725, 'TAGCG': 62551, 'TAGGA': 102471, 'TAGGT': 90393, 'TAGGC': 104839, 'TAGGG': 127198, 'TTAAA': 114923, 'TTAAT': 75440, 'TTAAC': 50665, 'TTAAG': 64885, 'TTATA': 45063, 'TTATT': 82837, 'TTATC': 54184, 'TTATG': 48843, 'TTACA': 81420, 'TTACT': 46693, 'TTACC': 41309, 'TTACG': 25734, 'TTAGA': 38106, 'TTAGT': 51959, 'TTAGC': 49875, 'TTAGG': 71643, 'TTTAA': 112238, 'TTTAT': 97251, 'TTTAC': 63030, 'TTTAG': 61317, 'TTTTA': 114773, 'TTTTT': 456427, 'TTTTC': 211355, 'TTTTG': 163054, 'TTTCA': 152696, 'TTTCT': 294895, 'TTTCC': 202026, 'TTTCG': 40574, 'TTTGA': 125900, 'TTTGT': 180692, 'TTTGC': 149105, 'TTTGG': 181526, 'TTCAA': 139031, 'TTCAT': 185835, 'TTCAC': 215172, 'TTCAG': 263572, 'TTCTA': 67598, 'TTCTT': 435222, 'TTCTC': 380905, 'TTCTG': 295797, 'TTCCA': 205498, 'TTCCT': 271160, 'TTCCC': 228295, 'TTCCG': 135896, 'TTCGA': 47468, 'TTCGT': 38415, 'TTCGC': 41884, 'TTCGG': 71253, 'TTGAA': 172901, 'TTGAT': 187235, 'TTGAC': 139120, 'TTGAG': 209412, 'TTGTA': 140724, 'TTGTT': 193750, 'TTGTC': 219804, 'TTGTG': 153538, 'TTGCA': 137470, 'TTGCT': 180869, 'TTGCC': 199886, 'TTGCG': 127017, 'TTGGA': 196981, 'TTGGT': 219577, 'TTGGC': 285614, 'TTGGG': 282794, 'TCAAA': 163318, 'TCAAT': 127954, 'TCAAC': 75174, 'TCAAG': 112723, 'TCATA': 96290, 'TCATT': 113571, 'TCATC': 288968, 'TCATG': 198429, 'TCACA': 189981, 'TCACT': 169894, 'TCACC': 216539, 'TCACG': 104425, 'TCAGA': 154420, 'TCAGT': 147184, 'TCAGC': 314355, 'TCAGG': 322029, 'TCTAA': 45375, 'TCTAT': 51347, 'TCTAC': 60461, 'TCTAG': 61396, 'TCTTA': 74580, 'TCTTT': 234868, 'TCTTC': 459948, 'TCTTG': 429619, 'TCTCA': 167844, 'TCTCT': 252084, 'TCTCC': 438731, 'TCTCG': 189970, 'TCTGA': 167296, 'TCTGT': 195858, 'TCTGC': 302935, 'TCTGG': 405391, 'TCCAA': 143138, 'TCCAT': 187627, 'TCCAC': 333941, 'TCCAG': 517192, 'TCCTA': 59050, 'TCCTT': 339910, 'TCCTC': 571554, 'TCCTG': 354977, 'TCCCA': 259957, 'TCCCT': 185442, 'TCCCC': 292514, 'TCCCG': 225786, 'TCCGA': 83076, 'TCCGT': 119160, 'TCCGC': 164398, 'TCCGG': 209061, 'TCGAA': 100371, 'TCGAT': 92344, 'TCGAC': 39516, 'TCGAG': 61682, 'TCGTA': 77806, 'TCGTT': 71523, 'TCGTC': 145965, 'TCGTG': 96754, 'TCGCA': 77947, 'TCGCT': 99173, 'TCGCC': 128330, 'TCGCG': 70482, 'TCGGA': 87062, 'TCGGT': 88150, 'TCGGC': 179604, 'TCGGG': 201333, 'TGAAA': 119505, 'TGAAT': 96244, 'TGAAC': 136396, 'TGAAG': 289651, 'TGATA': 58928, 'TGATT': 66548, 'TGATC': 153322, 'TGATG': 293069, 'TGACA': 141588, 'TGACT': 107383, 'TGACC': 178378, 'TGACG': 130872, 'TGAGA': 172424, 'TGAGT': 122245, 'TGAGC': 255938, 'TGAGG': 346689, 'TGTAA': 80024, 'TGTAT': 65839, 'TGTAC': 102357, 'TGTAG': 186675, 'TGTTA': 66134, 'TGTTT': 157372, 'TGTTC': 182527, 'TGTTG': 256831, 'TGTCA': 176853, 'TGTCT': 200316, 'TGTCC': 307811, 'TGTCG': 122244, 'TGTGA': 131989, 'TGTGT': 161369, 'TGTGC': 171919, 'TGTGG': 280568, 'TGCAA': 111099, 'TGCAT': 133112, 'TGCAC': 182523, 'TGCAG': 349874, 'TGCTA': 49808, 'TGCTT': 210975, 'TGCTC': 272337, 'TGCTG': 428362, 'TGCCA': 239995, 'TGCCT': 224940, 'TGCCC': 312848, 'TGCCG': 214433, 'TGCGA': 51013, 'TGCGT': 82251, 'TGCGC': 147691, 'TGCGG': 239431, 'TGGAA': 230147, 'TGGAT': 164248, 'TGGAC': 189528, 'TGGAG': 380902, 'TGGTA': 118954, 'TGGTT': 174813, 'TGGTC': 296920, 'TGGTG': 367467, 'TGGCA': 295055, 'TGGCT': 314418, 'TGGCC': 467207, 'TGGCG': 240369, 'TGGGA': 281750, 'TGGGT': 273436, 'TGGGC': 417354, 'TGGGG': 607592, 'CAAAA': 180067, 'CAAAT': 106320, 'CAAAC': 161525, 'CAAAG': 207707, 'CAATA': 58389, 'CAATT': 73911, 'CAATC': 93602, 'CAATG': 154069, 'CAACA': 120477, 'CAACT': 90432, 'CAACC': 95849, 'CAACG': 47827, 'CAAGA': 112247, 'CAAGT': 112885, 'CAAGC': 114011, 'CAAGG': 198292, 'CATAA': 67563, 'CATAT': 51667, 'CATAC': 90624, 'CATAG': 129354, 'CATTA': 61795, 'CATTT': 121509, 'CATTC': 143514, 'CATTG': 122866, 'CATCA': 217750, 'CATCT': 268686, 'CATCC': 237891, 'CATCG': 85914, 'CATGA': 193545, 'CATGT': 170974, 'CATGC': 164633, 'CATGG': 263511, 'CACAA': 166632, 'CACAT': 170038, 'CACAC': 214753, 'CACAG': 325941, 'CACTA': 47746, 'CACTT': 152566, 'CACTC': 172653, 'CACTG': 294471, 'CACCA': 354567, 'CACCT': 304777, 'CACCC': 245189, 'CACCG': 128161, 'CACGA': 114752, 'CACGT': 149338, 'CACGC': 162097, 'CACGG': 197751, 'CAGAA': 223837, 'CAGAT': 159489, 'CAGAC': 172044, 'CAGAG': 300242, 'CAGTA': 91437, 'CAGTT': 152991, 'CAGTC': 173378, 'CAGTG': 244414, 'CAGCA': 441688, 'CAGCT': 475856, 'CAGCC': 455392, 'CAGCG': 261981, 'CAGGA': 441414, 'CAGGT': 369423, 'CAGGC': 497467, 'CAGGG': 611679, 'CTAAA': 51546, 'CTAAT': 32491, 'CTAAC': 39687, 'CTAAG': 51348, 'CTATA': 22840, 'CTATT': 53079, 'CTATC': 43154, 'CTATG': 44403, 'CTACA': 56966, 'CTACT': 49726, 'CTACC': 48981, 'CTACG': 25508, 'CTAGA': 62152, 'CTAGT': 24454, 'CTAGC': 48336, 'CTAGG': 77832, 'CTTAA': 73031, 'CTTAT': 45164, 'CTTAC': 35397, 'CTTAG': 67593, 'CTTTA': 106843, 'CTTTT': 188778, 'CTTTC': 189417, 'CTTTG': 213237, 'CTTCA': 373430, 'CTTCT': 513918, 'CTTCC': 372027, 'CTTCG': 75953, 'CTTGA': 310542, 'CTTGT': 279655, 'CTTGC': 288172, 'CTTGG': 450814, 'CTCAA': 142926, 'CTCAT': 210927, 'CTCAC': 164370, 'CTCAG': 308339, 'CTCTA': 67807, 'CTCTT': 275597, 'CTCTC': 233615, 'CTCTG': 342006, 'CTCCA': 502688, 'CTCCT': 568029, 'CTCCC': 333349, 'CTCCG': 236069, 'CTCGA': 127787, 'CTCGT': 189498, 'CTCGC': 192722, 'CTCGG': 272683, 'CTGAA': 161473, 'CTGAT': 103165, 'CTGAC': 155369, 'CTGAG': 278736, 'CTGTA': 114185, 'CTGTT': 162393, 'CTGTC': 247879, 'CTGTG': 282810, 'CTGCA': 356511, 'CTGCT': 418888, 'CTGCC': 405891, 'CTGCG': 172678, 'CTGGA': 352331, 'CTGGT': 329776, 'CTGGC': 423376, 'CTGGG': 654175, 'CCAAA': 187080, 'CCAAT': 75194, 'CCAAC': 115628, 'CCAAG': 180439, 'CCATA': 89276, 'CCATT': 126916, 'CCATC': 217726, 'CCATG': 278149, 'CCACA': 295569, 'CCACT': 209631, 'CCACC': 421927, 'CCACG': 263518, 'CCAGA': 276049, 'CCAGT': 208987, 'CCAGC': 563380, 'CCAGG': 726698, 'CCTAA': 42476, 'CCTAT': 46555, 'CCTAC': 39047, 'CCTAG': 57352, 'CCTTA': 52855, 'CCTTT': 165754, 'CCTTC': 358499, 'CCTTG': 398756, 'CCTCA': 285746, 'CCTCT': 307748, 'CCTCC': 595654, 'CCTCG': 286437, 'CCTGA': 187519, 'CCTGT': 198173, 'CCTGC': 365515, 'CCTGG': 527924, 'CCCAA': 168718, 'CCCAT': 192769, 'CCCAC': 301267, 'CCCAG': 516871, 'CCCTA': 51277, 'CCCTT': 219050, 'CCCTC': 302118, 'CCCTG': 329711, 'CCCCA': 360997, 'CCCCT': 248969, 'CCCCC': 337109, 'CCCCG': 275560, 'CCCGA': 132324, 'CCCGT': 142927, 'CCCGC': 240761, 'CCCGG': 352955, 'CCGAA': 80767, 'CCGAT': 70940, 'CCGAC': 81536, 'CCGAG': 165069, 'CCGTA': 69329, 'CCGTT': 96369, 'CCGTC': 164786, 'CCGTG': 173642, 'CCGCA': 159358, 'CCGCT': 212532, 'CCGCC': 301448, 'CCGCG': 153891, 'CCGGA': 155274, 'CCGGT': 152493, 'CCGGC': 293637, 'CCGGG': 364112, 'CGAAA': 43819, 'CGAAT': 41274, 'CGAAC': 62726, 'CGAAG': 143840, 'CGATA': 19213, 'CGATT': 30276, 'CGATC': 79619, 'CGATG': 172748, 'CGACA': 54522, 'CGACT': 60681, 'CGACC': 74978, 'CGACG': 48073, 'CGAGA': 79218, 'CGAGT': 63271, 'CGAGC': 99942, 'CGAGG': 179769, 'CGTAA': 20439, 'CGTAT': 24541, 'CGTAC': 53229, 'CGTAG': 129969, 'CGTTA': 18004, 'CGTTT': 69811, 'CGTTC': 110523, 'CGTTG': 130264, 'CGTCA': 98524, 'CGTCT': 139012, 'CGTCC': 188752, 'CGTCG': 123015, 'CGTGA': 88119, 'CGTGT': 95080, 'CGTGC': 132566, 'CGTGG': 214417, 'CGCAA': 39713, 'CGCAT': 62382, 'CGCAC': 118985, 'CGCAG': 250133, 'CGCTA': 18338, 'CGCTT': 120213, 'CGCTC': 198236, 'CGCTG': 228112, 'CGCCA': 164504, 'CGCCT': 165289, 'CGCCC': 248361, 'CGCCG': 200671, 'CGCGA': 53387, 'CGCGT': 61477, 'CGCGC': 121544, 'CGCGG': 185898, 'CGGAA': 102759, 'CGGAT': 116963, 'CGGAC': 100189, 'CGGAG': 177574, 'CGGTA': 85795, 'CGGTT': 84539, 'CGGTC': 133791, 'CGGTG': 202209, 'CGGCA': 173242, 'CGGCT': 199490, 'CGGCC': 348912, 'CGGCG': 231390, 'CGGGA': 182934, 'CGGGT': 132995, 'CGGGC': 329989, 'CGGGG': 376123, 'GAAAA': 147758, 'GAAAT': 122691, 'GAAAC': 109509, 'GAAAG': 158251, 'GAATA': 58113, 'GAATT': 90792, 'GAATC': 96929, 'GAATG': 107054, 'GAACA': 142942, 'GAACT': 197997, 'GAACC': 117223, 'GAACG': 51564, 'GAAGA': 249179, 'GAAGT': 206969, 'GAAGC': 266097, 'GAAGG': 374755, 'GATAA': 53581, 'GATAT': 47624, 'GATAC': 50216, 'GATAG': 60791, 'GATTA': 34571, 'GATTT': 96497, 'GATTC': 78182, 'GATTG': 59805, 'GATCA': 132409, 'GATCT': 239013, 'GATCC': 127599, 'GATCG': 37608, 'GATGA': 256554, 'GATGT': 232793, 'GATGC': 204786, 'GATGG': 315961, 'GACAA': 96434, 'GACAT': 112778, 'GACAC': 139452, 'GACAG': 218372, 'GACTA': 30999, 'GACTT': 137815, 'GACTC': 146387, 'GACTG': 128477, 'GACCA': 194843, 'GACCT': 167827, 'GACCC': 181615, 'GACCG': 83157, 'GACGA': 81737, 'GACGT': 90193, 'GACGC': 101737, 'GACGG': 133038, 'GAGAA': 212018, 'GAGAT': 134041, 'GAGAC': 158042, 'GAGAG': 242757, 'GAGTA': 61429, 'GAGTT': 114636, 'GAGTC': 159889, 'GAGTG': 157418, 'GAGCA': 204460, 'GAGCT': 258638, 'GAGCC': 274540, 'GAGCG': 147302, 'GAGGA': 340487, 'GAGGT': 249526, 'GAGGC': 384599, 'GAGGG': 439316, 'GTAAA': 66115, 'GTAAT': 67119, 'GTAAC': 46039, 'GTAAG': 55050, 'GTATA': 36162, 'GTATT': 72394, 'GTATC': 49642, 'GTATG': 52098, 'GTACA': 114660, 'GTACT': 133148, 'GTACC': 69559, 'GTACG': 36063, 'GTAGA': 146788, 'GTAGT': 128730, 'GTAGC': 144289, 'GTAGG': 191213, 'GTTAA': 56778, 'GTTAT': 45430, 'GTTAC': 44983, 'GTTAG': 41682, 'GTTTA': 48677, 'GTTTT': 149718, 'GTTTC': 163541, 'GTTTG': 139097, 'GTTCA': 171468, 'GTTCT': 236920, 'GTTCC': 159465, 'GTTCG': 45872, 'GTTGA': 189837, 'GTTGT': 178928, 'GTTGC': 136442, 'GTTGG': 257423, 'GTCAA': 112956, 'GTCAT': 147447, 'GTCAC': 191895, 'GTCAG': 208522, 'GTCTA': 46724, 'GTCTT': 222588, 'GTCTC': 225125, 'GTCTG': 227534, 'GTCCA': 295293, 'GTCCT': 317549, 'GTCCC': 291311, 'GTCCG': 137619, 'GTCGA': 83311, 'GTCGT': 116283, 'GTCGC': 94980, 'GTCGG': 161294, 'GTGAA': 155291, 'GTGAT': 122099, 'GTGAC': 148768, 'GTGAG': 223074, 'GTGTA': 85703, 'GTGTT': 131279, 'GTGTC': 166242, 'GTGTG': 186525, 'GTGCA': 177786, 'GTGCT': 235185, 'GTGCC': 217281, 'GTGCG': 112721, 'GTGGA': 261181, 'GTGGT': 218940, 'GTGGC': 359148, 'GTGGG': 436791, 'GCAAA': 138486, 'GCAAT': 81844, 'GCAAC': 90823, 'GCAAG': 129907, 'GCATA': 75895, 'GCATT': 92311, 'GCATC': 172433, 'GCATG': 178384, 'GCACA': 212770, 'GCACT': 182432, 'GCACC': 229304, 'GCACG': 158434, 'GCAGA': 251077, 'GCAGT': 179822, 'GCAGC': 510807, 'GCAGG': 614831, 'GCTAA': 49707, 'GCTAT': 30279, 'GCTAC': 52660, 'GCTAG': 58259, 'GCTTA': 56558, 'GCTTT': 165417, 'GCTTC': 326983, 'GCTTG': 289672, 'GCTCA': 242409, 'GCTCT': 238922, 'GCTCC': 433367, 'GCTCG': 192110, 'GCTGA': 223681, 'GCTGT': 279548, 'GCTGC': 499260, 'GCTGG': 605849, 'GCCAA': 159999, 'GCCAT': 201323, 'GCCAC': 331269, 'GCCAG': 460482, 'GCCTA': 44208, 'GCCTT': 246180, 'GCCTC': 390277, 'GCCTG': 380087, 'GCCCA': 382019, 'GCCCT': 322011, 'GCCCC': 438433, 'GCCCG': 279533, 'GCCGA': 124668, 'GCCGT': 178354, 'GCCGC': 344246, 'GCCGG': 332890, 'GCGAA': 54802, 'GCGAT': 76995, 'GCGAC': 65259, 'GCGAG': 125555, 'GCGTA': 44684, 'GCGTT': 79352, 'GCGTC': 144153, 'GCGTG': 171574, 'GCGCA': 158492, 'GCGCT': 175889, 'GCGCC': 235489, 'GCGCG': 144868, 'GCGGA': 172872, 'GCGGT': 173549, 'GCGGC': 359660, 'GCGGG': 337635, 'GGAAA': 186010, 'GGAAT': 119552, 'GGAAC': 168824, 'GGAAG': 394571, 'GGATA': 53981, 'GGATT': 96194, 'GGATC': 188140, 'GGATG': 320115, 'GGACA': 228890, 'GGACT': 182058, 'GGACC': 216180, 'GGACG': 129790, 'GGAGA': 298205, 'GGAGT': 198584, 'GGAGC': 337449, 'GGAGG': 570499, 'GGTAA': 62556, 'GGTAT': 67392, 'GGTAC': 129272, 'GGTAG': 193039, 'GGTTA': 50081, 'GGTTT': 142891, 'GGTTC': 183658, 'GGTTG': 237224, 'GGTCA': 257100, 'GGTCT': 245552, 'GGTCC': 348425, 'GGTCG': 128387, 'GGTGA': 279760, 'GGTGT': 198382, 'GGTGC': 290727, 'GGTGG': 555039, 'GGCAA': 172196, 'GGCAT': 186491, 'GGCAC': 266921, 'GGCAG': 574805, 'GGCTA': 65133, 'GGCTT': 275594, 'GGCTC': 347293, 'GGCTG': 565621, 'GGCCA': 464145, 'GGCCT': 433572, 'GGCCC': 555374, 'GGCCG': 374667, 'GGCGA': 136795, 'GGCGT': 178409, 'GGCGC': 281663, 'GGCGG': 418590, 'GGGAA': 243793, 'GGGAT': 183917, 'GGGAC': 255021, 'GGGAG': 468132, 'GGGTA': 112792, 'GGGTT': 198911, 'GGGTC': 309394, 'GGGTG': 416486, 'GGGCA': 432937, 'GGGCT': 426149, 'GGGCC': 636881, 'GGGCG': 314919, 'GGGGA': 406274, 'GGGGT': 391439, 'GGGGC': 648178, 'GGGGG': 663218}, 'overrepresented_sequences': {}} {'total_reads': 2788791, 'total_bases': 161592785, 'q20_bases': 150786059, 'q30_bases': 141267514, 'total_cycles': 58, 'quality_curves': {'A': [36.8421, 36.8449, 36.8433, 36.8203, 36.8617, 36.8377, 36.7935, 36.7634, 36.6912, 36.6797, 36.7216, 36.7024, 36.659, 36.6205, 36.5713, 36.5826, 36.4799, 36.3673, 36.2835, 36.2402, 36.2164, 36.177, 36.0895, 36.077, 35.952, 35.9444, 35.8022, 35.7261, 35.7111, 35.4441, 35.3722, 35.1827, 34.9853, 34.8238, 34.808, 34.6352, 34.6054, 34.4153, 34.1035, 33.8057, 33.7085, 33.3015, 33.2508, 33.18, 33.0682, 32.8226, 32.4866, 32.2037, 31.8226, 31.768, 31.379, 30.9994, 30.4563, 30.1758, 30.1401, 29.4238, 28.9794, 28.36], 'T': [37.1311, 37.184, 37.1671, 37.132, 37.1401, 37.1202, 37.0723, 37.0249, 36.9741, 36.9254, 36.9816, 36.9353, 36.8802, 36.8564, 36.7727, 36.7846, 36.7, 36.5697, 36.4829, 36.4389, 36.4011, 36.3991, 36.2799, 36.2597, 36.097, 36.1237, 35.9915, 35.8927, 35.8369, 35.6294, 35.5408, 35.3581, 35.1647, 34.9742, 34.9616, 34.8136, 34.74, 34.5611, 34.192, 33.9497, 33.8073, 33.4391, 33.3408, 33.3295, 33.2565, 32.9635, 32.6145, 32.3044, 31.9441, 31.8814, 31.5457, 31.1676, 30.6958, 30.4697, 30.3127, 29.7491, 29.2199, 28.6632], 'C': [37.2027, 37.2125, 37.2202, 37.2255, 37.2135, 37.2053, 37.1932, 37.1781, 37.1374, 37.1125, 37.1153, 37.1304, 37.1173, 37.0774, 37.07, 37.0403, 37.0045, 36.93, 36.8662, 36.7797, 36.8167, 36.7844, 36.7299, 36.7179, 36.6354, 36.593, 36.5508, 36.4534, 36.411, 36.3062, 36.1847, 36.0323, 35.9143, 35.7744, 35.7232, 35.6057, 35.5404, 35.441, 35.2196, 34.895, 34.7286, 34.4352, 34.3355, 34.1974, 34.1152, 33.8698, 33.5466, 33.1708, 32.8152, 32.7058, 32.4134, 31.9587, 31.5176, 31.3059, 30.9904, 30.3996, 29.8895, 29.2293], 'G': [36.8168, 36.8615, 36.8668, 36.837, 36.8445, 36.8459, 36.811, 36.7516, 36.719, 36.6765, 36.7004, 36.7009, 36.6631, 36.6278, 36.574, 36.5563, 36.4779, 36.3975, 36.2955, 36.2394, 36.2092, 36.1819, 36.0894, 36.0821, 35.946, 35.9307, 35.8512, 35.7293, 35.6965, 35.5147, 35.3894, 35.2282, 35.056, 34.8796, 34.8495, 34.6965, 34.6542, 34.4842, 34.1878, 33.9334, 33.6967, 33.3374, 33.2527, 33.1515, 33.0685, 32.8326, 32.4624, 32.1495, 31.7233, 31.6369, 31.2946, 30.872, 30.387, 30.1407, 29.8958, 29.3159, 28.7023, 28.1754], 'mean': [37, 37.0247, 37.0256, 37.0036, 37.0137, 37.0034, 36.967, 36.9339, 36.8873, 36.8539, 36.8826, 36.8737, 36.8365, 36.803, 36.7598, 36.7501, 36.6768, 36.5825, 36.4984, 36.4381, 36.4246, 36.4003, 36.3155, 36.3063, 36.1816, 36.1683, 36.0774, 35.9752, 35.9376, 35.7604, 35.6513, 35.4787, 35.318, 35.1504, 35.1193, 34.9799, 34.9272, 34.7689, 34.4818, 34.1962, 34.0278, 33.6824, 33.5928, 33.5035, 33.4182, 33.1678, 32.8211, 32.5002, 32.1167, 32.0316, 31.7025, 31.2812, 30.8008, 30.5688, 30.3594, 29.751, 29.2257, 28.6287]}, 'content_curves': {'A': [0.212847, 0.218286, 0.214986, 0.219631, 0.226013, 0.216098, 0.218375, 0.220848, 0.217537, 0.219429, 0.223982, 0.220692, 0.219319, 0.223243, 0.215796, 0.218215, 0.222912, 0.21837, 0.218981, 0.222565, 0.219182, 0.219427, 0.221863, 0.215799, 0.220614, 0.221323, 0.216334, 0.220189, 0.222381, 0.218524, 0.221148, 0.223948, 0.217682, 0.2199, 0.223531, 0.215727, 0.217167, 0.223532, 0.219703, 0.220367, 0.2258, 0.21605, 0.220031, 0.220868, 0.219284, 0.218753, 0.223625, 0.217409, 0.22008, 0.221383, 0.21468, 0.216313, 0.219613, 0.214358, 0.218333, 0.221986, 0.214293, 0.217815], 'T': [0.205892, 0.199061, 0.198746, 0.192682, 0.195602, 0.193629, 0.191513, 0.199396, 0.196872, 0.19422, 0.2003, 0.196361, 0.194651, 0.198107, 0.197515, 0.195652, 0.197217, 0.200269, 0.195746, 0.198374, 0.194172, 0.191447, 0.197646, 0.193199, 0.194542, 0.1982, 0.19623, 0.195052, 0.199225, 0.194171, 0.1926, 0.197866, 0.195484, 0.194638, 0.197058, 0.197388, 0.192307, 0.195685, 0.19423, 0.191249, 0.193571, 0.194126, 0.192627, 0.199007, 0.192813, 0.191623, 0.195564, 0.192517, 0.191377, 0.197087, 0.194076, 0.190208, 0.197065, 0.195235, 0.192074, 0.194688, 0.192903, 0.191228], 'C': [0.2963, 0.295692, 0.295678, 0.291985, 0.291262, 0.295361, 0.293935, 0.293674, 0.296641, 0.294359, 0.291821, 0.294437, 0.294741, 0.293689, 0.296672, 0.296227, 0.294359, 0.297343, 0.29582, 0.295371, 0.293811, 0.295331, 0.294201, 0.300969, 0.297811, 0.296365, 0.299136, 0.296534, 0.293688, 0.302151, 0.297761, 0.292191, 0.29846, 0.296174, 0.294142, 0.300802, 0.301426, 0.297896, 0.302083, 0.300147, 0.297582, 0.30332, 0.298846, 0.298767, 0.300025, 0.301118, 0.298516, 0.302608, 0.301541, 0.297045, 0.304835, 0.30213, 0.299289, 0.305788, 0.301673, 0.301599, 0.306759, 0.303401], 'G': [0.284925, 0.286928, 0.290583, 0.295702, 0.287123, 0.294912, 0.296103, 0.286081, 0.28895, 0.291991, 0.283896, 0.28851, 0.291236, 0.284946, 0.290017, 0.289906, 0.2855, 0.283981, 0.289453, 0.283671, 0.292779, 0.293795, 0.286289, 0.290022, 0.28702, 0.284112, 0.288301, 0.288226, 0.284706, 0.285147, 0.288484, 0.285994, 0.288373, 0.289279, 0.285269, 0.286083, 0.2891, 0.282887, 0.283983, 0.288212, 0.283047, 0.286504, 0.288496, 0.281287, 0.287861, 0.288506, 0.282294, 0.287466, 0.287002, 0.284485, 0.286409, 0.291248, 0.284009, 0.284619, 0.28792, 0.281727, 0.286045, 0.287556], 'N': [3.62164e-05, 3.26306e-05, 7.53014e-06, 0, 0, 0, 7.38671e-05, 0, 0, 0, 0, 0, 5.30696e-05, 1.50603e-05, 0, 0, 1.21917e-05, 3.6575e-05, 1.07573e-06, 1.86461e-05, 5.59382e-05, 0, 0, 1.03988e-05, 1.18331e-05, 0, 0, 0, 0, 7.53026e-06, 7.53029e-06, 0, 0, 9.32449e-06, 0, 0, 0, 0, 0, 2.43954e-05, 0, 0, 1.43535e-06, 7.034e-05, 1.72281e-05, 0, 1.79512e-06, 0, 0, 0, 3.59479e-07, 0.000101061, 2.30322e-05, 0, 0, 0, 0, 0], 'GC': [0.581225, 0.58262, 0.58626, 0.587687, 0.578385, 0.590273, 0.590038, 0.579755, 0.585591, 0.586351, 0.575718, 0.582947, 0.585977, 0.578635, 0.586689, 0.586133, 0.579859, 0.581324, 0.585272, 0.579042, 0.58659, 0.589126, 0.580491, 0.590992, 0.584832, 0.580477, 0.587437, 0.584759, 0.578394, 0.587298, 0.586244, 0.578186, 0.586834, 0.585453, 0.579411, 0.586885, 0.590526, 0.580783, 0.586066, 0.58836, 0.580628, 0.589824, 0.587341, 0.580054, 0.587886, 0.589624, 0.58081, 0.590074, 0.588543, 0.58153, 0.591244, 0.593378, 0.583298, 0.590407, 0.589593, 0.583326, 0.592804, 0.590957]}, 'kmer_count': {'AAAAA': 232829, 'AAAAT': 107490, 'AAAAC': 129044, 'AAAAG': 149196, 'AAATA': 82445, 'AAATT': 85902, 'AAATC': 74683, 'AAATG': 101528, 'AAACA': 141968, 'AAACT': 102842, 'AAACC': 121417, 'AAACG': 57927, 'AAAGA': 201438, 'AAAGT': 111305, 'AAAGC': 139328, 'AAAGG': 137279, 'AATAA': 54210, 'AATAT': 51764, 'AATAC': 55225, 'AATAG': 35988, 'AATTA': 46258, 'AATTT': 88253, 'AATTC': 68382, 'AATTG': 54507, 'AATCA': 54945, 'AATCT': 67184, 'AATCC': 84610, 'AATCG': 22318, 'AATGA': 90114, 'AATGT': 95216, 'AATGC': 78807, 'AATGG': 110601, 'AACAA': 171383, 'AACAT': 143582, 'AACAC': 100618, 'AACAG': 146632, 'AACTA': 50810, 'AACTT': 109758, 'AACTC': 95743, 'AACTG': 131539, 'AACCA': 145063, 'AACCT': 133992, 'AACCC': 156359, 'AACCG': 65336, 'AACGA': 60691, 'AACGT': 64431, 'AACGC': 67538, 'AACGG': 79621, 'AAGAA': 377247, 'AAGAT': 210761, 'AAGAC': 193157, 'AAGAG': 241217, 'AAGTA': 91815, 'AAGTT': 132659, 'AAGTC': 128486, 'AAGTG': 129651, 'AAGCA': 177915, 'AAGCT': 205986, 'AAGCC': 227804, 'AAGCG': 114830, 'AAGGA': 284227, 'AAGGT': 157403, 'AAGGC': 215971, 'AAGGG': 178378, 'ATAAA': 68337, 'ATAAT': 32751, 'ATAAC': 37158, 'ATAAG': 36256, 'ATATA': 34206, 'ATATT': 50356, 'ATATC': 44302, 'ATATG': 42834, 'ATACA': 56580, 'ATACT': 43362, 'ATACC': 46943, 'ATACG': 20830, 'ATAGA': 37832, 'ATAGT': 29963, 'ATAGC': 28876, 'ATAGG': 33689, 'ATTAA': 53487, 'ATTAT': 40788, 'ATTAC': 55500, 'ATTAG': 22647, 'ATTTA': 58299, 'ATTTT': 127601, 'ATTTC': 98830, 'ATTTG': 80176, 'ATTCA': 83946, 'ATTCT': 82859, 'ATTCC': 86473, 'ATTCG': 40245, 'ATTGA': 102502, 'ATTGT': 74337, 'ATTGC': 70022, 'ATTGG': 59076, 'ATCAA': 156652, 'ATCAT': 133165, 'ATCAC': 101374, 'ATCAG': 92026, 'ATCTA': 60173, 'ATCTT': 123171, 'ATCTC': 120327, 'ATCTG': 137728, 'ATCCA': 146367, 'ATCCT': 163344, 'ATCCC': 147310, 'ATCCG': 110566, 'ATCGA': 86545, 'ATCGT': 54567, 'ATCGC': 61855, 'ATCGG': 55363, 'ATGAA': 164227, 'ATGAT': 135517, 'ATGAC': 124785, 'ATGAG': 185137, 'ATGTA': 56948, 'ATGTT': 84586, 'ATGTC': 114015, 'ATGTG': 151739, 'ATGCA': 112195, 'ATGCT': 115425, 'ATGCC': 167962, 'ATGCG': 55651, 'ATGGA': 176754, 'ATGGT': 122652, 'ATGGC': 193873, 'ATGGG': 159686, 'ACAAA': 146267, 'ACAAT': 59806, 'ACAAC': 145093, 'ACAAG': 261294, 'ACATA': 35799, 'ACATT': 86817, 'ACATC': 194838, 'ACATG': 153802, 'ACACA': 135196, 'ACACT': 84930, 'ACACC': 156855, 'ACACG': 79009, 'ACAGA': 175268, 'ACAGT': 108997, 'ACAGC': 235017, 'ACAGG': 168240, 'ACTAA': 39078, 'ACTAT': 51438, 'ACTAC': 107082, 'ACTAG': 20398, 'ACTTA': 38352, 'ACTTT': 109368, 'ACTTC': 173439, 'ACTTG': 82348, 'ACTCA': 98047, 'ACTCT': 94912, 'ACTCC': 165984, 'ACTCG': 55103, 'ACTGA': 124326, 'ACTGT': 111618, 'ACTGC': 164178, 'ACTGG': 183911, 'ACCAA': 188661, 'ACCAT': 174001, 'ACCAC': 174289, 'ACCAG': 284832, 'ACCTA': 77479, 'ACCTT': 122418, 'ACCTC': 203900, 'ACCTG': 317946, 'ACCCA': 216260, 'ACCCT': 196359, 'ACCCC': 302245, 'ACCCG': 114765, 'ACCGA': 74622, 'ACCGT': 69926, 'ACCGC': 162074, 'ACCGG': 122252, 'ACGAA': 36421, 'ACGAT': 46152, 'ACGAC': 93271, 'ACGAG': 163955, 'ACGTA': 20272, 'ACGTT': 27203, 'ACGTC': 72448, 'ACGTG': 129865, 'ACGCA': 81103, 'ACGCT': 115958, 'ACGCC': 161397, 'ACGCG': 58308, 'ACGGA': 115218, 'ACGGT': 51820, 'ACGGC': 154914, 'ACGGG': 114366, 'AGAAA': 237258, 'AGAAT': 102162, 'AGAAC': 194090, 'AGAAG': 454371, 'AGATA': 51519, 'AGATT': 97534, 'AGATC': 201446, 'AGATG': 237326, 'AGACA': 171158, 'AGACT': 119795, 'AGACC': 208488, 'AGACG': 133836, 'AGAGA': 224233, 'AGAGT': 121080, 'AGAGC': 227299, 'AGAGG': 264078, 'AGTAA': 37228, 'AGTAT': 66612, 'AGTAC': 119995, 'AGTAG': 47785, 'AGTTA': 48539, 'AGTTT': 109129, 'AGTTC': 172995, 'AGTTG': 76339, 'AGTCA': 102444, 'AGTCT': 87505, 'AGTCC': 162531, 'AGTCG': 58809, 'AGTGA': 149176, 'AGTGT': 95039, 'AGTGC': 151995, 'AGTGG': 201312, 'AGCAA': 149383, 'AGCAT': 101724, 'AGCAC': 191339, 'AGCAG': 376782, 'AGCTA': 70235, 'AGCTT': 115603, 'AGCTC': 227338, 'AGCTG': 425130, 'AGCCA': 285130, 'AGCCT': 268456, 'AGCCC': 362434, 'AGCCG': 194571, 'AGCGA': 95100, 'AGCGT': 77609, 'AGCGC': 164612, 'AGCGG': 221091, 'AGGAA': 218078, 'AGGAT': 147103, 'AGGAC': 275181, 'AGGAG': 500906, 'AGGTA': 57252, 'AGGTT': 89899, 'AGGTC': 151822, 'AGGTG': 273079, 'AGGCA': 190128, 'AGGCT': 201741, 'AGGCC': 376948, 'AGGCG': 161912, 'AGGGA': 161635, 'AGGGT': 128249, 'AGGGC': 263902, 'AGGGG': 195285, 'TAAAA': 83835, 'TAAAT': 45443, 'TAAAC': 42908, 'TAAAG': 85216, 'TAATA': 20972, 'TAATT': 45953, 'TAATC': 30118, 'TAATG': 50959, 'TAACA': 51906, 'TAACT': 46455, 'TAACC': 40127, 'TAACG': 13993, 'TAAGA': 61230, 'TAAGT': 31768, 'TAAGC': 40602, 'TAAGG': 43358, 'TATAA': 33451, 'TATAT': 42390, 'TATAC': 26768, 'TATAG': 22421, 'TATTA': 35051, 'TATTT': 104055, 'TATTC': 44706, 'TATTG': 47782, 'TATCA': 49429, 'TATCT': 66230, 'TATCC': 52059, 'TATCG': 16648, 'TATGA': 82758, 'TATGT': 66161, 'TATGC': 63788, 'TATGG': 75974, 'TACAA': 126632, 'TACAT': 74943, 'TACAC': 70992, 'TACAG': 98281, 'TACTA': 43608, 'TACTT': 67099, 'TACTC': 50191, 'TACTG': 87204, 'TACCA': 103676, 'TACCT': 110463, 'TACCC': 90437, 'TACCG': 75204, 'TACGA': 76579, 'TACGT': 29157, 'TACGC': 50627, 'TACGG': 57685, 'TAGAA': 47221, 'TAGAT': 28938, 'TAGAC': 36301, 'TAGAG': 64856, 'TAGTA': 21813, 'TAGTT': 27701, 'TAGTC': 30077, 'TAGTG': 44516, 'TAGCA': 41035, 'TAGCT': 49011, 'TAGCC': 55945, 'TAGCG': 18678, 'TAGGA': 51168, 'TAGGT': 25392, 'TAGGC': 35513, 'TAGGG': 38941, 'TTAAA': 80716, 'TTAAT': 47111, 'TTAAC': 43972, 'TTAAG': 52615, 'TTATA': 36030, 'TTATT': 77432, 'TTATC': 40793, 'TTATG': 58950, 'TTACA': 61639, 'TTACT': 56648, 'TTACC': 49154, 'TTACG': 15575, 'TTAGA': 35567, 'TTAGT': 30605, 'TTAGC': 44159, 'TTAGG': 35639, 'TTTAA': 83699, 'TTTAT': 92662, 'TTTAC': 46437, 'TTTAG': 43595, 'TTTTA': 112646, 'TTTTT': 232937, 'TTTTC': 114409, 'TTTTG': 137656, 'TTTCA': 107260, 'TTTCT': 149978, 'TTTCC': 142770, 'TTTCG': 43089, 'TTTGA': 133584, 'TTTGT': 130671, 'TTTGC': 118415, 'TTTGG': 152241, 'TTCAA': 150609, 'TTCAT': 113513, 'TTCAC': 132740, 'TTCAG': 151212, 'TTCTA': 82285, 'TTCTT': 139507, 'TTCTC': 181853, 'TTCTG': 181575, 'TTCCA': 175304, 'TTCCT': 238357, 'TTCCC': 190095, 'TTCCG': 94377, 'TTCGA': 85589, 'TTCGT': 58049, 'TTCGC': 53805, 'TTCGG': 78533, 'TTGAA': 106683, 'TTGAT': 70927, 'TTGAC': 98627, 'TTGAG': 118566, 'TTGTA': 57452, 'TTGTT': 85339, 'TTGTC': 86982, 'TTGTG': 136751, 'TTGCA': 91324, 'TTGCT': 103935, 'TTGCC': 136261, 'TTGCG': 40162, 'TTGGA': 118356, 'TTGGT': 74798, 'TTGGC': 124985, 'TTGGG': 136459, 'TCAAA': 118184, 'TCAAT': 57028, 'TCAAC': 161929, 'TCAAG': 254393, 'TCATA': 38373, 'TCATT': 81920, 'TCATC': 205234, 'TCATG': 168943, 'TCACA': 110482, 'TCACT': 120612, 'TCACC': 233585, 'TCACG': 73910, 'TCAGA': 152763, 'TCAGT': 104553, 'TCAGC': 199278, 'TCAGG': 162174, 'TCTAA': 33617, 'TCTAT': 54171, 'TCTAC': 136746, 'TCTAG': 51520, 'TCTTA': 48279, 'TCTTT': 140651, 'TCTTC': 224075, 'TCTTG': 102322, 'TCTCA': 141778, 'TCTCT': 166337, 'TCTCC': 251575, 'TCTCG': 76660, 'TCTGA': 131484, 'TCTGT': 155028, 'TCTGC': 214644, 'TCTGG': 248893, 'TCCAA': 164926, 'TCCAT': 115056, 'TCCAC': 210712, 'TCCAG': 301738, 'TCCTA': 83101, 'TCCTT': 144506, 'TCCTC': 272471, 'TCCTG': 388388, 'TCCCA': 233430, 'TCCCT': 213596, 'TCCCC': 311532, 'TCCCG': 157449, 'TCCGA': 88446, 'TCCGT': 69335, 'TCCGC': 165150, 'TCCGG': 157418, 'TCGAA': 41282, 'TCGAT': 31498, 'TCGAC': 80393, 'TCGAG': 122381, 'TCGTA': 21732, 'TCGTT': 22663, 'TCGTC': 71182, 'TCGTG': 113649, 'TCGCA': 47103, 'TCGCT': 69076, 'TCGCC': 116168, 'TCGCG': 53380, 'TCGGA': 85194, 'TCGGT': 54254, 'TCGGC': 130948, 'TCGGG': 120385, 'TGAAA': 128654, 'TGAAT': 79663, 'TGAAC': 143938, 'TGAAG': 323655, 'TGATA': 45122, 'TGATT': 71137, 'TGATC': 123066, 'TGATG': 196669, 'TGACA': 144149, 'TGACT': 103962, 'TGACC': 232809, 'TGACG': 88346, 'TGAGA': 150394, 'TGAGT': 107927, 'TGAGC': 217515, 'TGAGG': 259481, 'TGTAA': 66277, 'TGTAT': 62309, 'TGTAC': 86557, 'TGTAG': 50580, 'TGTTA': 52487, 'TGTTT': 129767, 'TGTTC': 125220, 'TGTTG': 102030, 'TGTCA': 121268, 'TGTCT': 132850, 'TGTCC': 200134, 'TGTCG': 60134, 'TGTGA': 168289, 'TGTGT': 153985, 'TGTGC': 193798, 'TGTGG': 264509, 'TGCAA': 116875, 'TGCAT': 84589, 'TGCAC': 144937, 'TGCAG': 338435, 'TGCTA': 60945, 'TGCTT': 118093, 'TGCTC': 172776, 'TGCTG': 422920, 'TGCCA': 238205, 'TGCCT': 256283, 'TGCCC': 346248, 'TGCCG': 158574, 'TGCGA': 65925, 'TGCGT': 66894, 'TGCGC': 149510, 'TGCGG': 157912, 'TGGAA': 174566, 'TGGAT': 144457, 'TGGAC': 281994, 'TGGAG': 455901, 'TGGTA': 61404, 'TGGTT': 91531, 'TGGTC': 174047, 'TGGTG': 320105, 'TGGCA': 205848, 'TGGCT': 244486, 'TGGCC': 418912, 'TGGCG': 171919, 'TGGGA': 229207, 'TGGGT': 151823, 'TGGGC': 332328, 'TGGGG': 299405, 'CAAAA': 145282, 'CAAAT': 93786, 'CAAAC': 115375, 'CAAAG': 189075, 'CAATA': 42640, 'CAATT': 50458, 'CAATC': 56244, 'CAATG': 108310, 'CAACA': 214616, 'CAACT': 114974, 'CAACC': 185749, 'CAACG': 109425, 'CAAGA': 358014, 'CAAGT': 180438, 'CAAGC': 247926, 'CAAGG': 348692, 'CATAA': 39457, 'CATAT': 34785, 'CATAC': 44776, 'CATAG': 38316, 'CATTA': 48289, 'CATTT': 90566, 'CATTC': 90658, 'CATTG': 126659, 'CATCA': 249449, 'CATCT': 205743, 'CATCC': 257525, 'CATCG': 149467, 'CATGA': 174415, 'CATGT': 130743, 'CATGC': 159053, 'CATGG': 255423, 'CACAA': 126997, 'CACAT': 97888, 'CACAC': 146999, 'CACAG': 237316, 'CACTA': 63457, 'CACTT': 111491, 'CACTC': 117614, 'CACTG': 210422, 'CACCA': 308518, 'CACCT': 263023, 'CACCC': 315031, 'CACCG': 164392, 'CACGA': 79934, 'CACGT': 74227, 'CACGC': 156771, 'CACGG': 148411, 'CAGAA': 240798, 'CAGAT': 171674, 'CAGAC': 201376, 'CAGAG': 301119, 'CAGTA': 79544, 'CAGTT': 125709, 'CAGTC': 119624, 'CAGTG': 252067, 'CAGCA': 359381, 'CAGCT': 319065, 'CAGCC': 500478, 'CAGCG': 228539, 'CAGGA': 300449, 'CAGGT': 194206, 'CAGGC': 325347, 'CAGGG': 264261, 'CTAAA': 57580, 'CTAAT': 31630, 'CTAAC': 30806, 'CTAAG': 56252, 'CTATA': 32626, 'CTATT': 54168, 'CTATC': 59080, 'CTATG': 112428, 'CTACA': 159974, 'CTACT': 85532, 'CTACC': 179304, 'CTACG': 130426, 'CTAGA': 49711, 'CTAGT': 32044, 'CTAGC': 45981, 'CTAGG': 46217, 'CTTAA': 48675, 'CTTAT': 47294, 'CTTAC': 41139, 'CTTAG': 46753, 'CTTTA': 52587, 'CTTTT': 138533, 'CTTTC': 141618, 'CTTTG': 183274, 'CTTCA': 236016, 'CTTCT': 224677, 'CTTCC': 332911, 'CTTCG': 124994, 'CTTGA': 98644, 'CTTGT': 90359, 'CTTGC': 108044, 'CTTGG': 142118, 'CTCAA': 173233, 'CTCAT': 142244, 'CTCAC': 179930, 'CTCAG': 233718, 'CTCTA': 75313, 'CTCTT': 149560, 'CTCTC': 193217, 'CTCTG': 263558, 'CTCCA': 303326, 'CTCCT': 302065, 'CTCCC': 357410, 'CTCCG': 178631, 'CTCGA': 57602, 'CTCGT': 54471, 'CTCGC': 100286, 'CTCGG': 167548, 'CTGAA': 221575, 'CTGAT': 133490, 'CTGAC': 183272, 'CTGAG': 273226, 'CTGTA': 94012, 'CTGTT': 128913, 'CTGTC': 189795, 'CTGTG': 292681, 'CTGCA': 313120, 'CTGCT': 366593, 'CTGCC': 470574, 'CTGCG': 221907, 'CTGGA': 457420, 'CTGGT': 248187, 'CTGGC': 415198, 'CTGGG': 451062, 'CCAAA': 157983, 'CCAAT': 77431, 'CCAAC': 203593, 'CCAAG': 366460, 'CCATA': 55750, 'CCATT': 107166, 'CCATC': 288067, 'CCATG': 258041, 'CCACA': 224734, 'CCACT': 175924, 'CCACC': 418152, 'CCACG': 186732, 'CCAGA': 328430, 'CCAGT': 196183, 'CCAGC': 515307, 'CCAGG': 438904, 'CCTAA': 55080, 'CCTAT': 82215, 'CCTAC': 173894, 'CCTAG': 60235, 'CCTTA': 59156, 'CCTTT': 144436, 'CCTTC': 304140, 'CCTTG': 153213, 'CCTCA': 286026, 'CCTCT': 245839, 'CCTCC': 442796, 'CCTCG': 158753, 'CCTGA': 277149, 'CCTGT': 212069, 'CCTGC': 510709, 'CCTGG': 636218, 'CCCAA': 218422, 'CCCAT': 168495, 'CCCAC': 317222, 'CCCAG': 527131, 'CCCTA': 107972, 'CCCTT': 190473, 'CCCTC': 317388, 'CCCTG': 496766, 'CCCCA': 436795, 'CCCCT': 348818, 'CCCCC': 520794, 'CCCCG': 303677, 'CCCGA': 173445, 'CCCGT': 111616, 'CCCGC': 270333, 'CCCGG': 313916, 'CCGAA': 69588, 'CCGAT': 49767, 'CCGAC': 144655, 'CCGAG': 247989, 'CCGTA': 31941, 'CCGTT': 43403, 'CCGTC': 124233, 'CCGTG': 170244, 'CCGCA': 189725, 'CCGCT': 182153, 'CCGCC': 393656, 'CCGCG': 185221, 'CCGGA': 187550, 'CCGGT': 69531, 'CCGGC': 283561, 'CCGGG': 314010, 'CGAAA': 36047, 'CGAAT': 36101, 'CGAAC': 43501, 'CGAAG': 75949, 'CGATA': 20274, 'CGATT': 33697, 'CGATC': 35208, 'CGATG': 84942, 'CGACA': 106838, 'CGACT': 75695, 'CGACC': 130467, 'CGACG': 100018, 'CGAGA': 164765, 'CGAGT': 101295, 'CGAGC': 172806, 'CGAGG': 265811, 'CGTAA': 20994, 'CGTAT': 15774, 'CGTAC': 33569, 'CGTAG': 22060, 'CGTTA': 10779, 'CGTTT': 36027, 'CGTTC': 42575, 'CGTTG': 42947, 'CGTCA': 105423, 'CGTCT': 100272, 'CGTCC': 122039, 'CGTCG': 48289, 'CGTGA': 91776, 'CGTGT': 84564, 'CGTGC': 156794, 'CGTGG': 233912, 'CGCAA': 117108, 'CGCAT': 90739, 'CGCAC': 101029, 'CGCAG': 150285, 'CGCTA': 62765, 'CGCTT': 86073, 'CGCTC': 126069, 'CGCTG': 246867, 'CGCCA': 227008, 'CGCCT': 214907, 'CGCCC': 265035, 'CGCCG': 217734, 'CGCGA': 74544, 'CGCGT': 58861, 'CGCGC': 142262, 'CGCGG': 162199, 'CGGAA': 133068, 'CGGAT': 65752, 'CGGAC': 127108, 'CGGAG': 246506, 'CGGTA': 26533, 'CGGTT': 41580, 'CGGTC': 69782, 'CGGTG': 114910, 'CGGCA': 188356, 'CGGCT': 179503, 'CGGCC': 343988, 'CGGCG': 233783, 'CGGGA': 199801, 'CGGGT': 91449, 'CGGGC': 245306, 'CGGGG': 244018, 'GAAAA': 159397, 'GAAAT': 98432, 'GAAAC': 137327, 'GAAAG': 166241, 'GAATA': 51118, 'GAATT': 75056, 'GAATC': 68561, 'GAATG': 114055, 'GAACA': 154115, 'GAACT': 123501, 'GAACC': 153687, 'GAACG': 91051, 'GAAGA': 404069, 'GAAGT': 157948, 'GAAGC': 299636, 'GAAGG': 306939, 'GATAA': 47542, 'GATAT': 43249, 'GATAC': 40340, 'GATAG': 33570, 'GATTA': 43006, 'GATTT': 82638, 'GATTC': 89578, 'GATTG': 77333, 'GATCA': 129315, 'GATCT': 101384, 'GATCC': 173995, 'GATCG': 69598, 'GATGA': 261705, 'GATGT': 115937, 'GATGC': 149379, 'GATGG': 210291, 'GACAA': 188824, 'GACAT': 153950, 'GACAC': 137595, 'GACAG': 204755, 'GACTA': 59919, 'GACTT': 115129, 'GACTC': 150631, 'GACTG': 153056, 'GACCA': 266173, 'GACCT': 214978, 'GACCC': 272123, 'GACCG': 124968, 'GACGA': 122921, 'GACGT': 82603, 'GACGC': 142025, 'GACGG': 150807, 'GAGAA': 323860, 'GAGAT': 176705, 'GAGAC': 201899, 'GAGAG': 228115, 'GAGTA': 78774, 'GAGTT': 120403, 'GAGTC': 132751, 'GAGTG': 170903, 'GAGCA': 239748, 'GAGCT': 265004, 'GAGCC': 328295, 'GAGCG': 197199, 'GAGGA': 505508, 'GAGGT': 194174, 'GAGGC': 354421, 'GAGGG': 267596, 'GTAAA': 51381, 'GTAAT': 36622, 'GTAAC': 40667, 'GTAAG': 32265, 'GTATA': 22062, 'GTATT': 50673, 'GTATC': 40129, 'GTATG': 74431, 'GTACA': 91788, 'GTACT': 62033, 'GTACC': 104915, 'GTACG': 46526, 'GTAGA': 53860, 'GTAGT': 31092, 'GTAGC': 46055, 'GTAGG': 35394, 'GTTAA': 38583, 'GTTAT': 32444, 'GTTAC': 39762, 'GTTAG': 31965, 'GTTTA': 42249, 'GTTTT': 97688, 'GTTTC': 88812, 'GTTTG': 134896, 'GTTCA': 119836, 'GTTCT': 126513, 'GTTCC': 135312, 'GTTCG': 66535, 'GTTGA': 60400, 'GTTGT': 70974, 'GTTGC': 74491, 'GTTGG': 100441, 'GTCAA': 109853, 'GTCAT': 104024, 'GTCAC': 123471, 'GTCAG': 141188, 'GTCTA': 57721, 'GTCTT': 101975, 'GTCTC': 139180, 'GTCTG': 164769, 'GTCCA': 166316, 'GTCCT': 185295, 'GTCCC': 221780, 'GTCCG': 94258, 'GTCGA': 44799, 'GTCGT': 62182, 'GTCGC': 69374, 'GTCGG': 86672, 'GTGAA': 182687, 'GTGAT': 95500, 'GTGAC': 162136, 'GTGAG': 155263, 'GTGTA': 57152, 'GTGTT': 108960, 'GTGTC': 122076, 'GTGTG': 197247, 'GTGCA': 166620, 'GTGCT': 188704, 'GTGCC': 226428, 'GTGCG': 122208, 'GTGGA': 302024, 'GTGGT': 201224, 'GTGGC': 305174, 'GTGGG': 265001, 'GCAAA': 121839, 'GCAAT': 63591, 'GCAAC': 116146, 'GCAAG': 253011, 'GCATA': 27039, 'GCATT': 79444, 'GCATC': 175443, 'GCATG': 137849, 'GCACA': 139557, 'GCACT': 121670, 'GCACC': 244925, 'GCACG': 119985, 'GCAGA': 258474, 'GCAGT': 167401, 'GCAGC': 457313, 'GCAGG': 315929, 'GCTAA': 48458, 'GCTAT': 70568, 'GCTAC': 136680, 'GCTAG': 41661, 'GCTTA': 38047, 'GCTTT': 121468, 'GCTTC': 217764, 'GCTTG': 100359, 'GCTCA': 201739, 'GCTCT': 174123, 'GCTCC': 283624, 'GCTCG': 88252, 'GCTGA': 278268, 'GCTGT': 224041, 'GCTGC': 483799, 'GCTGG': 502117, 'GCCAA': 234725, 'GCCAT': 250025, 'GCCAC': 303738, 'GCCAG': 365629, 'GCCTA': 103005, 'GCCTT': 203732, 'GCCTC': 338486, 'GCCTG': 431706, 'GCCCA': 347628, 'GCCCT': 355271, 'GCCCC': 488022, 'GCCCG': 294837, 'GCCGA': 175392, 'GCCGT': 119639, 'GCCGC': 352358, 'GCCGG': 261186, 'GCGAA': 44413, 'GCGAT': 46632, 'GCGAC': 94393, 'GCGAG': 170278, 'GCGTA': 18273, 'GCGTT': 38651, 'GCGTC': 108169, 'GCGTG': 154066, 'GCGCA': 140736, 'GCGCT': 153852, 'GCGCC': 255245, 'GCGCG': 139316, 'GCGGA': 181117, 'GCGGT': 77031, 'GCGGC': 375307, 'GCGGG': 233039, 'GGAAA': 158988, 'GGAAT': 90838, 'GGAAC': 141218, 'GGAAG': 317000, 'GGATA': 47757, 'GGATT': 90396, 'GGATC': 110872, 'GGATG': 217843, 'GGACA': 262446, 'GGACT': 179276, 'GGACC': 308870, 'GGACG': 176183, 'GGAGA': 389962, 'GGAGT': 173391, 'GGAGC': 410256, 'GGAGG': 531191, 'GGTAA': 36707, 'GGTAT': 42947, 'GGTAC': 65190, 'GGTAG': 46096, 'GGTTA': 31356, 'GGTTT': 89689, 'GGTTC': 106946, 'GGTTG': 84115, 'GGTCA': 147796, 'GGTCT': 142106, 'GGTCC': 183902, 'GGTCG': 95395, 'GGTGA': 185099, 'GGTGT': 151824, 'GGTGC': 202068, 'GGTGG': 374660, 'GGCAA': 171851, 'GGCAT': 142495, 'GGCAC': 187540, 'GGCAG': 333637, 'GGCTA': 103337, 'GGCTT': 158139, 'GGCTC': 223414, 'GGCTG': 395550, 'GGCCA': 404387, 'GGCCT': 340260, 'GGCCC': 517252, 'GGCCG': 338222, 'GGCGA': 118914, 'GGCGT': 116175, 'GGCGC': 232234, 'GGCGG': 322623, 'GGGAA': 182410, 'GGGAT': 109948, 'GGGAC': 242590, 'GGGAG': 299281, 'GGGTA': 46332, 'GGGTT': 89187, 'GGGTC': 172196, 'GGGTG': 204156, 'GGGCA': 252533, 'GGGCT': 254489, 'GGGCC': 462062, 'GGGCG': 218721, 'GGGGA': 244899, 'GGGGT': 140862, 'GGGGC': 349590, 'GGGGG': 249975}, 'overrepresented_sequences': {}} {'passed_filter_reads': 5577582, 'low_quality_reads': 351860, 'too_many_N_reads': 664, 'too_short_reads': 54, 'too_long_reads': 0} fastp -i /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045018_1.fastq.gz -I /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045018_2.fastq.gz -o /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045018.trimmed_1.fastq.gz -O /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045018.trimmed_2.fastq.gz -l 25 --adapter_fasta /home/ubuntu/workspace/rnaseq/team_exercise/references/illumina_multiplex.fa --trim_tail1 5 --trim_tail2 5 --json /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045018.fastp.json --html /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045018.fastp.html SRR10045019_1 {'adapter_trimmed_reads': 44744, 'adapter_trimmed_bases': 279569, 'read1_adapter_sequence': 'unspecified', 'read2_adapter_sequence': 'unspecified', 'read1_adapter_counts': {'A': 699, 'C': 796, 'G': 864, 'T': 729, 'CC': 224, 'GG': 293, 'others': 18766}, 'read2_adapter_counts': {'A': 831, 'C': 847, 'G': 768, 'T': 642, 'CC': 262, 'others': 19022}} {'rate': 0.141819} {'peak': 85, 'unknown': 1050794, 'histogram': [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 66, 49, 52, 49, 49, 53, 61, 56, 57, 96, 85, 97, 117, 141, 158, 177, 237, 264, 321, 353, 421, 496, 570, 653, 758, 883, 1027, 1266, 1306, 1519, 1597, 1847, 2063, 2207, 2329, 2647, 2850, 3078, 3302, 3581, 3786, 4132, 4343, 4557, 4837, 5030, 5362, 5625, 5783, 6216, 6305, 6541, 6849, 6812, 7090, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 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229561, 'ACTAA': 52166, 'ACTAT': 68808, 'ACTAC': 151381, 'ACTAG': 27564, 'ACTTA': 51001, 'ACTTT': 145788, 'ACTTC': 237028, 'ACTTG': 111323, 'ACTCA': 133517, 'ACTCT': 130609, 'ACTCC': 227720, 'ACTCG': 75206, 'ACTGA': 167776, 'ACTGT': 148945, 'ACTGC': 223774, 'ACTGG': 251358, 'ACCAA': 261544, 'ACCAT': 238231, 'ACCAC': 242152, 'ACCAG': 392028, 'ACCTA': 103650, 'ACCTT': 168690, 'ACCTC': 278117, 'ACCTG': 429583, 'ACCCA': 300093, 'ACCCT': 268691, 'ACCCC': 432715, 'ACCCG': 159145, 'ACCGA': 101526, 'ACCGT': 96214, 'ACCGC': 225487, 'ACCGG': 173834, 'ACGAA': 51104, 'ACGAT': 61742, 'ACGAC': 133190, 'ACGAG': 229954, 'ACGTA': 28086, 'ACGTT': 37464, 'ACGTC': 100571, 'ACGTG': 179637, 'ACGCA': 114441, 'ACGCT': 163656, 'ACGCC': 223408, 'ACGCG': 81530, 'ACGGA': 153593, 'ACGGT': 71967, 'ACGGC': 211728, 'ACGGG': 158981, 'AGAAA': 327070, 'AGAAT': 138023, 'AGAAC': 269422, 'AGAAG': 627323, 'AGATA': 72841, 'AGATT': 132997, 'AGATC': 280394, 'AGATG': 325417, 'AGACA': 240291, 'AGACT': 161495, 'AGACC': 283767, 'AGACG': 183362, 'AGAGA': 312010, 'AGAGT': 163081, 'AGAGC': 314053, 'AGAGG': 367220, 'AGTAA': 50232, 'AGTAT': 89472, 'AGTAC': 163609, 'AGTAG': 62957, 'AGTTA': 62813, 'AGTTT': 148754, 'AGTTC': 231057, 'AGTTG': 102491, 'AGTCA': 136397, 'AGTCT': 120377, 'AGTCC': 220416, 'AGTCG': 81014, 'AGTGA': 205113, 'AGTGT': 128561, 'AGTGC': 206346, 'AGTGG': 274564, 'AGCAA': 205415, 'AGCAT': 139170, 'AGCAC': 264721, 'AGCAG': 521143, 'AGCTA': 95872, 'AGCTT': 154933, 'AGCTC': 309340, 'AGCTG': 589189, 'AGCCA': 393204, 'AGCCT': 363109, 'AGCCC': 505464, 'AGCCG': 269781, 'AGCGA': 134501, 'AGCGT': 106845, 'AGCGC': 230609, 'AGCGG': 309466, 'AGGAA': 301966, 'AGGAT': 198521, 'AGGAC': 376683, 'AGGAG': 699194, 'AGGTA': 76423, 'AGGTT': 121777, 'AGGTC': 208663, 'AGGTG': 375077, 'AGGCA': 257954, 'AGGCT': 272829, 'AGGCC': 516152, 'AGGCG': 223698, 'AGGGA': 218112, 'AGGGT': 174857, 'AGGGC': 363693, 'AGGGG': 269812, 'TAAAA': 115805, 'TAAAT': 61464, 'TAAAC': 59969, 'TAAAG': 113862, 'TAATA': 28201, 'TAATT': 60379, 'TAATC': 39384, 'TAATG': 67607, 'TAACA': 69047, 'TAACT': 61944, 'TAACC': 54802, 'TAACG': 19621, 'TAAGA': 81955, 'TAAGT': 41322, 'TAAGC': 55498, 'TAAGG': 59323, 'TATAA': 44859, 'TATAT': 58031, 'TATAC': 36433, 'TATAG': 28934, 'TATTA': 47963, 'TATTT': 136753, 'TATTC': 60949, 'TATTG': 65298, 'TATCA': 67147, 'TATCT': 87892, 'TATCC': 70599, 'TATCG': 22936, 'TATGA': 110205, 'TATGT': 88481, 'TATGC': 85968, 'TATGG': 101760, 'TACAA': 177155, 'TACAT': 101986, 'TACAC': 97218, 'TACAG': 134394, 'TACTA': 59263, 'TACTT': 88655, 'TACTC': 68690, 'TACTG': 117889, 'TACCA': 141841, 'TACCT': 148981, 'TACCC': 127755, 'TACCG': 102211, 'TACGA': 105022, 'TACGT': 41122, 'TACGC': 67836, 'TACGG': 77967, 'TAGAA': 64487, 'TAGAT': 38273, 'TAGAC': 49585, 'TAGAG': 87011, 'TAGTA': 28478, 'TAGTT': 36171, 'TAGTC': 39595, 'TAGTG': 61984, 'TAGCA': 54655, 'TAGCT': 64145, 'TAGCC': 73265, 'TAGCG': 25413, 'TAGGA': 67892, 'TAGGT': 33512, 'TAGGC': 48650, 'TAGGG': 52305, 'TTAAA': 107778, 'TTAAT': 60199, 'TTAAC': 60407, 'TTAAG': 71857, 'TTATA': 48327, 'TTATT': 101771, 'TTATC': 55261, 'TTATG': 78054, 'TTACA': 84193, 'TTACT': 74893, 'TTACC': 67848, 'TTACG': 21257, 'TTAGA': 47925, 'TTAGT': 40890, 'TTAGC': 56331, 'TTAGG': 46556, 'TTTAA': 110778, 'TTTAT': 123495, 'TTTAC': 62705, 'TTTAG': 57016, 'TTTTA': 147691, 'TTTTT': 333734, 'TTTTC': 157354, 'TTTTG': 186288, 'TTTCA': 145644, 'TTTCT': 203667, 'TTTCC': 195435, 'TTTCG': 57931, 'TTTGA': 182607, 'TTTGT': 178588, 'TTTGC': 155997, 'TTTGG': 206471, 'TTCAA': 206169, 'TTCAT': 156263, 'TTCAC': 179390, 'TTCAG': 204358, 'TTCTA': 108018, 'TTCTT': 190386, 'TTCTC': 248284, 'TTCTG': 245466, 'TTCCA': 238341, 'TTCCT': 323824, 'TTCCC': 260619, 'TTCCG': 127206, 'TTCGA': 117611, 'TTCGT': 77019, 'TTCGC': 72610, 'TTCGG': 110155, 'TTGAA': 141677, 'TTGAT': 93565, 'TTGAC': 133033, 'TTGAG': 163251, 'TTGTA': 75447, 'TTGTT': 114712, 'TTGTC': 120821, 'TTGTG': 186261, 'TTGCA': 122291, 'TTGCT': 136620, 'TTGCC': 181099, 'TTGCG': 54503, 'TTGGA': 160218, 'TTGGT': 100718, 'TTGGC': 172884, 'TTGGG': 184671, 'TCAAA': 163510, 'TCAAT': 77161, 'TCAAC': 219359, 'TCAAG': 341069, 'TCATA': 51204, 'TCATT': 110725, 'TCATC': 282492, 'TCATG': 226247, 'TCACA': 150668, 'TCACT': 164373, 'TCACC': 318992, 'TCACG': 104590, 'TCAGA': 205812, 'TCAGT': 142221, 'TCAGC': 270642, 'TCAGG': 222119, 'TCTAA': 43617, 'TCTAT': 73648, 'TCTAC': 181527, 'TCTAG': 65375, 'TCTTA': 63713, 'TCTTT': 193040, 'TCTTC': 306330, 'TCTTG': 137137, 'TCTCA': 190858, 'TCTCT': 224199, 'TCTCC': 349360, 'TCTCG': 107023, 'TCTGA': 178055, 'TCTGT': 211437, 'TCTGC': 292426, 'TCTGG': 337943, 'TCCAA': 227436, 'TCCAT': 160154, 'TCCAC': 292016, 'TCCAG': 412486, 'TCCTA': 113875, 'TCCTT': 195584, 'TCCTC': 380575, 'TCCTG': 530533, 'TCCCA': 318740, 'TCCCT': 293464, 'TCCCC': 441561, 'TCCCG': 216806, 'TCCGA': 119085, 'TCCGT': 97855, 'TCCGC': 223768, 'TCCGG': 213382, 'TCGAA': 57025, 'TCGAT': 43180, 'TCGAC': 111787, 'TCGAG': 168715, 'TCGTA': 28888, 'TCGTT': 30264, 'TCGTC': 98923, 'TCGTG': 153855, 'TCGCA': 64618, 'TCGCT': 98453, 'TCGCC': 161419, 'TCGCG': 75719, 'TCGGA': 121025, 'TCGGT': 73573, 'TCGGC': 182907, 'TCGGG': 168441, 'TGAAA': 177017, 'TGAAT': 107929, 'TGAAC': 193820, 'TGAAG': 437919, 'TGATA': 59989, 'TGATT': 95289, 'TGATC': 164086, 'TGATG': 264691, 'TGACA': 195896, 'TGACT': 141156, 'TGACC': 313737, 'TGACG': 120166, 'TGAGA': 205784, 'TGAGT': 140882, 'TGAGC': 299257, 'TGAGG': 355944, 'TGTAA': 88534, 'TGTAT': 81362, 'TGTAC': 116010, 'TGTAG': 67565, 'TGTTA': 67031, 'TGTTT': 174410, 'TGTTC': 169476, 'TGTTG': 139290, 'TGTCA': 164874, 'TGTCT': 181406, 'TGTCC': 274257, 'TGTCG': 84919, 'TGTGA': 225844, 'TGTGT': 206084, 'TGTGC': 260316, 'TGTGG': 363611, 'TGCAA': 154805, 'TGCAT': 115195, 'TGCAC': 201274, 'TGCAG': 461787, 'TGCTA': 82810, 'TGCTT': 156643, 'TGCTC': 240215, 'TGCTG': 582176, 'TGCCA': 322995, 'TGCCT': 348983, 'TGCCC': 479173, 'TGCCG': 217909, 'TGCGA': 91506, 'TGCGT': 89301, 'TGCGC': 203681, 'TGCGG': 217404, 'TGGAA': 240604, 'TGGAT': 195024, 'TGGAC': 382419, 'TGGAG': 626351, 'TGGTA': 85534, 'TGGTT': 123067, 'TGGTC': 229221, 'TGGTG': 440202, 'TGGCA': 280216, 'TGGCT': 333394, 'TGGCC': 569778, 'TGGCG': 240518, 'TGGGA': 311773, 'TGGGT': 203007, 'TGGGC': 458914, 'TGGGG': 410534, 'CAAAA': 209408, 'CAAAT': 127722, 'CAAAC': 158340, 'CAAAG': 262558, 'CAATA': 57524, 'CAATT': 65491, 'CAATC': 74785, 'CAATG': 146877, 'CAACA': 294807, 'CAACT': 157044, 'CAACC': 255767, 'CAACG': 151964, 'CAAGA': 495918, 'CAAGT': 241817, 'CAAGC': 336056, 'CAAGG': 477207, 'CATAA': 53213, 'CATAT': 46936, 'CATAC': 60749, 'CATAG': 51256, 'CATTA': 66350, 'CATTT': 122304, 'CATTC': 122563, 'CATTG': 168444, 'CATCA': 334992, 'CATCT': 271996, 'CATCC': 356375, 'CATCG': 206277, 'CATGA': 237806, 'CATGT': 175605, 'CATGC': 215250, 'CATGG': 351517, 'CACAA': 180795, 'CACAT': 134505, 'CACAC': 210612, 'CACAG': 328838, 'CACTA': 89240, 'CACTT': 152181, 'CACTC': 162950, 'CACTG': 288236, 'CACCA': 425428, 'CACCT': 360485, 'CACCC': 449017, 'CACCG': 231142, 'CACGA': 114347, 'CACGT': 102978, 'CACGC': 223119, 'CACGG': 202746, 'CAGAA': 329422, 'CAGAT': 233586, 'CAGAC': 276315, 'CAGAG': 409619, 'CAGTA': 108768, 'CAGTT': 173074, 'CAGTC': 164782, 'CAGTG': 344235, 'CAGCA': 497924, 'CAGCT': 439106, 'CAGCC': 689149, 'CAGCG': 319213, 'CAGGA': 413493, 'CAGGT': 268829, 'CAGGC': 443692, 'CAGGG': 367900, 'CTAAA': 78511, 'CTAAT': 42083, 'CTAAC': 41194, 'CTAAG': 73439, 'CTATA': 43184, 'CTATT': 73427, 'CTATC': 80495, 'CTATG': 151188, 'CTACA': 221186, 'CTACT': 117042, 'CTACC': 247118, 'CTACG': 180113, 'CTAGA': 67529, 'CTAGT': 42048, 'CTAGC': 59299, 'CTAGG': 62446, 'CTTAA': 65612, 'CTTAT': 62968, 'CTTAC': 56878, 'CTTAG': 61886, 'CTTTA': 70635, 'CTTTT': 188507, 'CTTTC': 191760, 'CTTTG': 246363, 'CTTCA': 324160, 'CTTCT': 302941, 'CTTCC': 456696, 'CTTCG': 175522, 'CTTGA': 130320, 'CTTGT': 120618, 'CTTGC': 146136, 'CTTGG': 191654, 'CTCAA': 237850, 'CTCAT': 192801, 'CTCAC': 247227, 'CTCAG': 319893, 'CTCTA': 102729, 'CTCTT': 201740, 'CTCTC': 267953, 'CTCTG': 367608, 'CTCCA': 419737, 'CTCCT': 415018, 'CTCCC': 505577, 'CTCCG': 246721, 'CTCGA': 82503, 'CTCGT': 76256, 'CTCGC': 143947, 'CTCGG': 232231, 'CTGAA': 302243, 'CTGAT': 180246, 'CTGAC': 247601, 'CTGAG': 372449, 'CTGTA': 127105, 'CTGTT': 174444, 'CTGTC': 263770, 'CTGTG': 396230, 'CTGCA': 429451, 'CTGCT': 508862, 'CTGCC': 653831, 'CTGCG': 305593, 'CTGGA': 624305, 'CTGGT': 337686, 'CTGGC': 564671, 'CTGGG': 621037, 'CCAAA': 222761, 'CCAAT': 102325, 'CCAAC': 280055, 'CCAAG': 505078, 'CCATA': 75172, 'CCATT': 146015, 'CCATC': 388102, 'CCATG': 353425, 'CCACA': 317846, 'CCACT': 242153, 'CCACC': 594763, 'CCACG': 259658, 'CCAGA': 451819, 'CCAGT': 270789, 'CCAGC': 706353, 'CCAGG': 601720, 'CCTAA': 75060, 'CCTAT': 110774, 'CCTAC': 240700, 'CCTAG': 82344, 'CCTTA': 80397, 'CCTTT': 195701, 'CCTTC': 418880, 'CCTTG': 205011, 'CCTCA': 392967, 'CCTCT': 340571, 'CCTCC': 621111, 'CCTCG': 226929, 'CCTGA': 376243, 'CCTGT': 289532, 'CCTGC': 704398, 'CCTGG': 859512, 'CCCAA': 303913, 'CCCAT': 227723, 'CCCAC': 456142, 'CCCAG': 726296, 'CCCTA': 148844, 'CCCTT': 262084, 'CCCTC': 452930, 'CCCTG': 677149, 'CCCCA': 611933, 'CCCCT': 492317, 'CCCCC': 997558, 'CCCCG': 444015, 'CCCGA': 234676, 'CCCGT': 153682, 'CCCGC': 398630, 'CCCGG': 443778, 'CCGAA': 95727, 'CCGAT': 65631, 'CCGAC': 199560, 'CCGAG': 336852, 'CCGTA': 43406, 'CCGTT': 60741, 'CCGTC': 172501, 'CCGTG': 235912, 'CCGCA': 263101, 'CCGCT': 255547, 'CCGCC': 567684, 'CCGCG': 263703, 'CCGGA': 259459, 'CCGGT': 97024, 'CCGGC': 402626, 'CCGGG': 439640, 'CGAAA': 49863, 'CGAAT': 48701, 'CGAAC': 60755, 'CGAAG': 107490, 'CGATA': 25786, 'CGATT': 45963, 'CGATC': 47661, 'CGATG': 116098, 'CGACA': 147701, 'CGACT': 105445, 'CGACC': 182002, 'CGACG': 139368, 'CGAGA': 229904, 'CGAGT': 139095, 'CGAGC': 240794, 'CGAGG': 361967, 'CGTAA': 28045, 'CGTAT': 20890, 'CGTAC': 46686, 'CGTAG': 30743, 'CGTTA': 14227, 'CGTTT': 48999, 'CGTTC': 59019, 'CGTTG': 57834, 'CGTCA': 141071, 'CGTCT': 136749, 'CGTCC': 172798, 'CGTCG': 69933, 'CGTGA': 124765, 'CGTGT': 114944, 'CGTGC': 216554, 'CGTGG': 320917, 'CGCAA': 157496, 'CGCAT': 122329, 'CGCAC': 142980, 'CGCAG': 212603, 'CGCTA': 89196, 'CGCTT': 120007, 'CGCTC': 178787, 'CGCTG': 348830, 'CGCCA': 310389, 'CGCCT': 295616, 'CGCCC': 384947, 'CGCCG': 313698, 'CGCGA': 104066, 'CGCGT': 81214, 'CGCGC': 201540, 'CGCGG': 233675, 'CGGAA': 181235, 'CGGAT': 91154, 'CGGAC': 178425, 'CGGAG': 339202, 'CGGTA': 36716, 'CGGTT': 56649, 'CGGTC': 96845, 'CGGTG': 160502, 'CGGCA': 263407, 'CGGCT': 251241, 'CGGCC': 485115, 'CGGCG': 334582, 'CGGGA': 278471, 'CGGGT': 126954, 'CGGGC': 345897, 'CGGGG': 347027, 'GAAAA': 225319, 'GAAAT': 134848, 'GAAAC': 187645, 'GAAAG': 223962, 'GAATA': 69605, 'GAATT': 100883, 'GAATC': 93138, 'GAATG': 154852, 'GAACA': 208487, 'GAACT': 166846, 'GAACC': 211846, 'GAACG': 126511, 'GAAGA': 567347, 'GAAGT': 211767, 'GAAGC': 411791, 'GAAGG': 416515, 'GATAA': 64611, 'GATAT': 57803, 'GATAC': 55799, 'GATAG': 46088, 'GATTA': 57268, 'GATTT': 112231, 'GATTC': 119454, 'GATTG': 104184, 'GATCA': 175841, 'GATCT': 136992, 'GATCC': 233681, 'GATCG': 96164, 'GATGA': 354720, 'GATGT': 156308, 'GATGC': 206899, 'GATGG': 285794, 'GACAA': 259591, 'GACAT': 208258, 'GACAC': 189537, 'GACAG': 286608, 'GACTA': 81781, 'GACTT': 158009, 'GACTC': 203830, 'GACTG': 207885, 'GACCA': 365982, 'GACCT': 288648, 'GACCC': 371357, 'GACCG': 172880, 'GACGA': 173769, 'GACGT': 113660, 'GACGC': 195568, 'GACGG': 205557, 'GAGAA': 446644, 'GAGAT': 244276, 'GAGAC': 275700, 'GAGAG': 318468, 'GAGTA': 107097, 'GAGTT': 161634, 'GAGTC': 180458, 'GAGTG': 228687, 'GAGCA': 330219, 'GAGCT': 364592, 'GAGCC': 455546, 'GAGCG': 280077, 'GAGGA': 702101, 'GAGGT': 265711, 'GAGGC': 487406, 'GAGGG': 365519, 'GTAAA': 69121, 'GTAAT': 49732, 'GTAAC': 54086, 'GTAAG': 43390, 'GTATA': 28933, 'GTATT': 67159, 'GTATC': 54211, 'GTATG': 98045, 'GTACA': 126745, 'GTACT': 84552, 'GTACC': 141189, 'GTACG': 61688, 'GTAGA': 71236, 'GTAGT': 42995, 'GTAGC': 63169, 'GTAGG': 48595, 'GTTAA': 50548, 'GTTAT': 41997, 'GTTAC': 53047, 'GTTAG': 41284, 'GTTTA': 57135, 'GTTTT': 132506, 'GTTTC': 122670, 'GTTTG': 180742, 'GTTCA': 160773, 'GTTCT': 171326, 'GTTCC': 183646, 'GTTCG': 90119, 'GTTGA': 80024, 'GTTGT': 97981, 'GTTGC': 99581, 'GTTGG': 138098, 'GTCAA': 147192, 'GTCAT': 139379, 'GTCAC': 170930, 'GTCAG': 187794, 'GTCTA': 73602, 'GTCTT': 143697, 'GTCTC': 192132, 'GTCTG': 222764, 'GTCCA': 229143, 'GTCCT': 254053, 'GTCCC': 307380, 'GTCCG': 129977, 'GTCGA': 61055, 'GTCGT': 82731, 'GTCGC': 99512, 'GTCGG': 120319, 'GTGAA': 249895, 'GTGAT': 127811, 'GTGAC': 222231, 'GTGAG': 209233, 'GTGTA': 74993, 'GTGTT': 146842, 'GTGTC': 168233, 'GTGTG': 264612, 'GTGCA': 227843, 'GTGCT': 256544, 'GTGCC': 309993, 'GTGCG': 165799, 'GTGGA': 416904, 'GTGGT': 270861, 'GTGGC': 420510, 'GTGGG': 364865, 'GCAAA': 162724, 'GCAAT': 84426, 'GCAAC': 157088, 'GCAAG': 345594, 'GCATA': 36355, 'GCATT': 107605, 'GCATC': 233238, 'GCATG': 191444, 'GCACA': 195705, 'GCACT': 168913, 'GCACC': 339784, 'GCACG': 167327, 'GCAGA': 352214, 'GCAGT': 229624, 'GCAGC': 638875, 'GCAGG': 440074, 'GCTAA': 65279, 'GCTAT': 96175, 'GCTAC': 192730, 'GCTAG': 55970, 'GCTTA': 52250, 'GCTTT': 162828, 'GCTTC': 301031, 'GCTTG': 133912, 'GCTCA': 278216, 'GCTCT': 242701, 'GCTCC': 393421, 'GCTCG': 123215, 'GCTGA': 378558, 'GCTGT': 309270, 'GCTGC': 675181, 'GCTGG': 697334, 'GCCAA': 318677, 'GCCAT': 334773, 'GCCAC': 424651, 'GCCAG': 499082, 'GCCTA': 141637, 'GCCTT': 274365, 'GCCTC': 469925, 'GCCTG': 591333, 'GCCCA': 483075, 'GCCCT': 484591, 'GCCCC': 699549, 'GCCCG': 409398, 'GCCGA': 242411, 'GCCGT': 164666, 'GCCGC': 501529, 'GCCGG': 369516, 'GCGAA': 62617, 'GCGAT': 65147, 'GCGAC': 132654, 'GCGAG': 237906, 'GCGTA': 25783, 'GCGTT': 51478, 'GCGTC': 148083, 'GCGTG': 210395, 'GCGCA': 191238, 'GCGCT': 217891, 'GCGCC': 357777, 'GCGCG': 197825, 'GCGGA': 250414, 'GCGGT': 107295, 'GCGGC': 532826, 'GCGGG': 333208, 'GGAAA': 218064, 'GGAAT': 124171, 'GGAAC': 191527, 'GGAAG': 435990, 'GGATA': 64981, 'GGATT': 119247, 'GGATC': 151903, 'GGATG': 296444, 'GGACA': 357966, 'GGACT': 243509, 'GGACC': 421859, 'GGACG': 245721, 'GGAGA': 539220, 'GGAGT': 234122, 'GGAGC': 572665, 'GGAGG': 733866, 'GGTAA': 49337, 'GGTAT': 57530, 'GGTAC': 89105, 'GGTAG': 65060, 'GGTTA': 42479, 'GGTTT': 122432, 'GGTTC': 145645, 'GGTTG': 114415, 'GGTCA': 199488, 'GGTCT': 191492, 'GGTCC': 256000, 'GGTCG': 127329, 'GGTGA': 252952, 'GGTGT': 205367, 'GGTGC': 277818, 'GGTGG': 514938, 'GGCAA': 232589, 'GGCAT': 192257, 'GGCAC': 264096, 'GGCAG': 464577, 'GGCTA': 141869, 'GGCTT': 218781, 'GGCTC': 310187, 'GGCTG': 540291, 'GGCCA': 545931, 'GGCCT': 468966, 'GGCCC': 720061, 'GGCCG': 475379, 'GGCGA': 166487, 'GGCGT': 158696, 'GGCGC': 329233, 'GGCGG': 461537, 'GGGAA': 247855, 'GGGAT': 148341, 'GGGAC': 333394, 'GGGAG': 412965, 'GGGTA': 62675, 'GGGTT': 122695, 'GGGTC': 239145, 'GGGTG': 275250, 'GGGCA': 350203, 'GGGCT': 350333, 'GGGCC': 644317, 'GGGCG': 311655, 'GGGGA': 335339, 'GGGGT': 194927, 'GGGGC': 490753, 'GGGGG': 366110}, 'overrepresented_sequences': {}} {'total_reads': 3434219, 'total_bases': 199007113, 'q20_bases': 194276522, 'q30_bases': 188508692, 'total_cycles': 58, 'quality_curves': {'A': [37.2366, 37.2465, 37.2419, 37.2819, 37.2566, 37.2709, 37.2484, 37.2567, 37.1733, 37.1327, 37.2032, 37.208, 37.1837, 37.148, 37.0717, 37.0821, 37.074, 37.0283, 37.0505, 37.08, 37.0888, 37.076, 37.0516, 36.9724, 36.8647, 36.7177, 36.6396, 36.5858, 36.4894, 36.4876, 36.4283, 36.4027, 36.3571, 36.1806, 36.1871, 36.1621, 36.0945, 36.0951, 36.0131, 35.9637, 35.8877, 35.5938, 35.8032, 35.7178, 35.7927, 35.7641, 35.7305, 35.6575, 35.7136, 35.6374, 35.5137, 35.4097, 35.3915, 35.2988, 35.1596, 35.1288, 35.0694, 34.9271], 'T': [37.5457, 37.5268, 37.5298, 37.5272, 37.5011, 37.5017, 37.467, 37.4752, 37.397, 37.3702, 37.383, 37.3866, 37.3847, 37.3579, 37.3529, 37.3672, 37.3707, 37.3118, 37.3098, 37.3109, 37.3409, 37.3229, 37.318, 37.2568, 37.2054, 37.0932, 37.0128, 36.9861, 36.9068, 36.8812, 36.8671, 36.8476, 36.7764, 36.6691, 36.6685, 36.623, 36.6201, 36.5826, 36.5032, 36.4447, 36.3891, 36.112, 36.2802, 36.2235, 36.2262, 36.2369, 36.1808, 36.1428, 36.1527, 36.0628, 35.9078, 35.8117, 35.7559, 35.696, 35.578, 35.5256, 35.4887, 35.3489], 'C': [37.4027, 37.4287, 37.3879, 37.3904, 37.4002, 37.3914, 37.4065, 37.4006, 37.3265, 37.3431, 37.3574, 37.3814, 37.3566, 37.3826, 37.3234, 37.3066, 37.3327, 37.3307, 37.3219, 37.3163, 37.3046, 37.31, 37.2846, 37.2365, 37.1505, 37.0896, 37.0041, 36.946, 36.9079, 36.8492, 36.8355, 36.8401, 36.7723, 36.7043, 36.6948, 36.6477, 36.6379, 36.6192, 36.5659, 36.5251, 36.4983, 36.2171, 36.3717, 36.407, 36.3958, 36.3907, 36.3952, 36.3479, 36.3409, 36.3104, 36.213, 36.1279, 36.0678, 35.9868, 35.9377, 35.8501, 35.8364, 35.6708], 'G': [37.1931, 37.2232, 37.1957, 37.2291, 37.2029, 37.1959, 37.1976, 37.2235, 37.1356, 37.1482, 37.1852, 37.1981, 37.1568, 37.1476, 37.0849, 37.0717, 37.0794, 37.0684, 37.0771, 37.1103, 37.095, 37.09, 37.0618, 36.9719, 36.9022, 36.808, 36.7143, 36.6769, 36.6194, 36.5557, 36.5592, 36.5521, 36.49, 36.3885, 36.3709, 36.3511, 36.3444, 36.3329, 36.2501, 36.2223, 36.1559, 35.9239, 36.0849, 36.0532, 36.0749, 36.1069, 36.0586, 35.9709, 36.0044, 35.9645, 35.8402, 35.7362, 35.7, 35.639, 35.5508, 35.4978, 35.4289, 35.3095], 'mean': [37.3436, 37.3538, 37.3346, 37.3508, 37.3345, 37.3339, 37.3252, 37.3358, 37.2551, 37.2497, 37.2808, 37.2933, 37.2688, 37.2595, 37.2079, 37.2047, 37.2132, 37.1875, 37.1926, 37.2068, 37.2068, 37.2004, 37.1791, 37.1092, 37.0326, 36.934, 36.8469, 36.8041, 36.7396, 36.6967, 36.6805, 36.6702, 36.607, 36.4997, 36.4942, 36.4575, 36.4403, 36.4231, 36.3471, 36.3064, 36.2524, 35.9819, 36.1542, 36.1278, 36.1435, 36.1479, 36.1183, 36.0532, 36.0757, 36.0202, 35.8958, 35.8001, 35.7579, 35.6823, 35.5896, 35.5328, 35.4853, 35.3468]}, 'content_curves': {'A': [0.200358, 0.19362, 0.201033, 0.201342, 0.196884, 0.204051, 0.199226, 0.195493, 0.204528, 0.197284, 0.199398, 0.204603, 0.202626, 0.196589, 0.205211, 0.200758, 0.197942, 0.203481, 0.199412, 0.195395, 0.202844, 0.198959, 0.195697, 0.200691, 0.195881, 0.194215, 0.200973, 0.195077, 0.193865, 0.19905, 0.19498, 0.193814, 0.199834, 0.196862, 0.193169, 0.200532, 0.19707, 0.194971, 0.202137, 0.196628, 0.195106, 0.200349, 0.196636, 0.192623, 0.198542, 0.198141, 0.193261, 0.199335, 0.194136, 0.194746, 0.197848, 0.193957, 0.194621, 0.199049, 0.196935, 0.194931, 0.199264, 0.195333], 'T': [0.247205, 0.230196, 0.236664, 0.228067, 0.223145, 0.230974, 0.226524, 0.225649, 0.231121, 0.232199, 0.222975, 0.228915, 0.229667, 0.219993, 0.225333, 0.225264, 0.219481, 0.225563, 0.227244, 0.222211, 0.22487, 0.226601, 0.221092, 0.225402, 0.224761, 0.220899, 0.225328, 0.226588, 0.222229, 0.226871, 0.22658, 0.221155, 0.227822, 0.228777, 0.223993, 0.227792, 0.226637, 0.221639, 0.222649, 0.22507, 0.220481, 0.226374, 0.226291, 0.22309, 0.225779, 0.228077, 0.222054, 0.226849, 0.226645, 0.22278, 0.222141, 0.226935, 0.218009, 0.221142, 0.222948, 0.217318, 0.22245, 0.219532], 'C': [0.260508, 0.273223, 0.263668, 0.26688, 0.275903, 0.266175, 0.270356, 0.276831, 0.26898, 0.271787, 0.278706, 0.273226, 0.271238, 0.278775, 0.275201, 0.273974, 0.279891, 0.275774, 0.277283, 0.280505, 0.275337, 0.274387, 0.281267, 0.275819, 0.280442, 0.286107, 0.277226, 0.278392, 0.282683, 0.27505, 0.278771, 0.283571, 0.27744, 0.278233, 0.284448, 0.277709, 0.281634, 0.283724, 0.280554, 0.280583, 0.284528, 0.277992, 0.280555, 0.286048, 0.281941, 0.279436, 0.285022, 0.280699, 0.279909, 0.281934, 0.282255, 0.280983, 0.287533, 0.283011, 0.283628, 0.286254, 0.281515, 0.286066], 'G': [0.291929, 0.302961, 0.298631, 0.303711, 0.304068, 0.2988, 0.303895, 0.302027, 0.295371, 0.29873, 0.29892, 0.293255, 0.296468, 0.304644, 0.294246, 0.300004, 0.302686, 0.295182, 0.296061, 0.301888, 0.296949, 0.300053, 0.301944, 0.298088, 0.298916, 0.298779, 0.296473, 0.299943, 0.301223, 0.299028, 0.299668, 0.301459, 0.294904, 0.296128, 0.298389, 0.293967, 0.29466, 0.299665, 0.294661, 0.297718, 0.299885, 0.295286, 0.296519, 0.298239, 0.293739, 0.294347, 0.299663, 0.293117, 0.29931, 0.30054, 0.297756, 0.298125, 0.299836, 0.296798, 0.296488, 0.301497, 0.29677, 0.299068], 'N': [0, 0, 4.65899e-06, 0, 0, 0, 0, 0, 0, 0, 1.16475e-06, 1.74712e-06, 0, 0, 9.31798e-06, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1.46681e-06], 'GC': [0.552437, 0.576184, 0.562298, 0.570591, 0.579971, 0.564975, 0.574251, 0.578859, 0.564351, 0.570517, 0.577627, 0.566481, 0.567706, 0.583419, 0.569447, 0.573978, 0.582577, 0.570956, 0.573344, 0.582394, 0.572286, 0.574441, 0.583211, 0.573907, 0.579358, 0.584886, 0.5737, 0.578335, 0.583906, 0.574078, 0.578439, 0.585031, 0.572344, 0.574361, 0.582838, 0.571676, 0.576293, 0.58339, 0.575215, 0.578301, 0.584413, 0.573277, 0.577074, 0.584288, 0.575679, 0.573782, 0.584685, 0.573816, 0.579219, 0.582473, 0.580011, 0.579108, 0.587369, 0.579809, 0.580117, 0.587751, 0.578285, 0.585134]}, 'kmer_count': {'AAAAA': 353615, 'AAAAT': 177704, 'AAAAC': 135645, 'AAAAG': 175080, 'AAATA': 149246, 'AAATT': 119782, 'AAATC': 111983, 'AAATG': 130142, 'AAACA': 165370, 'AAACT': 148094, 'AAACC': 121935, 'AAACG': 47969, 'AAAGA': 158880, 'AAAGT': 136212, 'AAAGC': 156645, 'AAAGG': 174767, 'AATAA': 117096, 'AATAT': 74781, 'AATAC': 62835, 'AATAG': 67937, 'AATTA': 66016, 'AATTT': 125370, 'AATTC': 99818, 'AATTG': 67036, 'AATCA': 92267, 'AATCT': 137714, 'AATCC': 108343, 'AATCG': 39419, 'AATGA': 110982, 'AATGT': 108950, 'AATGC': 107309, 'AATGG': 152855, 'AACAA': 115372, 'AACAT': 110304, 'AACAC': 131366, 'AACAG': 160058, 'AACTA': 40580, 'AACTT': 172025, 'AACTC': 147378, 'AACTG': 158273, 'AACCA': 110606, 'AACCT': 104912, 'AACCC': 116103, 'AACCG': 43716, 'AACGA': 26247, 'AACGT': 35863, 'AACGC': 43744, 'AACGG': 57028, 'AAGAA': 176371, 'AAGAT': 132753, 'AAGAC': 110653, 'AAGAG': 185958, 'AAGTA': 88449, 'AAGTT': 131901, 'AAGTC': 146203, 'AAGTG': 161519, 'AAGCA': 140891, 'AAGCT': 150573, 'AAGCC': 216135, 'AAGCG': 104438, 'AAGGA': 187823, 'AAGGT': 157061, 'AAGGC': 253078, 'AAGGG': 242524, 'ATAAA': 128791, 'ATAAT': 59833, 'ATAAC': 43121, 'ATAAG': 59630, 'ATATA': 59069, 'ATATT': 72395, 'ATATC': 48438, 'ATATG': 49194, 'ATACA': 88588, 'ATACT': 83693, 'ATACC': 48714, 'ATACG': 19182, 'ATAGA': 75397, 'ATAGT': 71192, 'ATAGC': 83445, 'ATAGG': 86742, 'ATTAA': 62741, 'ATTAT': 45181, 'ATTAC': 55409, 'ATTAG': 42279, 'ATTTA': 64180, 'ATTTT': 153849, 'ATTTC': 132628, 'ATTTG': 128979, 'ATTCA': 109470, 'ATTCT': 134925, 'ATTCC': 117468, 'ATTCG': 42186, 'ATTGA': 82611, 'ATTGT': 70679, 'ATTGC': 77113, 'ATTGG': 101782, 'ATCAA': 87634, 'ATCAT': 160891, 'ATCAC': 118189, 'ATCAG': 165682, 'ATCTA': 37823, 'ATCTT': 289516, 'ATCTC': 224424, 'ATCTG': 219920, 'ATCCA': 184803, 'ATCCT': 169977, 'ATCCC': 119346, 'ATCCG': 76805, 'ATCGA': 36849, 'ATCGT': 50699, 'ATCGC': 51778, 'ATCGG': 51560, 'ATGAA': 162648, 'ATGAT': 170165, 'ATGAC': 122166, 'ATGAG': 202661, 'ATGTA': 101112, 'ATGTT': 190490, 'ATGTC': 184967, 'ATGTG': 129978, 'ATGCA': 105963, 'ATGCT': 132530, 'ATGCC': 178818, 'ATGCG': 118708, 'ATGGA': 156392, 'ATGGT': 199374, 'ATGGC': 262284, 'ATGGG': 218196, 'ACAAA': 180251, 'ACAAT': 103537, 'ACAAC': 79066, 'ACAAG': 123004, 'ACATA': 82147, 'ACATT': 124016, 'ACATC': 141713, 'ACATG': 148416, 'ACACA': 192326, 'ACACT': 112909, 'ACACC': 179017, 'ACACG': 107604, 'ACAGA': 184805, 'ACAGT': 132605, 'ACAGC': 271837, 'ACAGG': 281396, 'ACTAA': 39594, 'ACTAT': 37112, 'ACTAC': 32252, 'ACTAG': 37928, 'ACTTA': 38631, 'ACTTT': 141772, 'ACTTC': 203366, 'ACTTG': 221919, 'ACTCA': 132220, 'ACTCT': 128859, 'ACTCC': 188321, 'ACTCG': 127015, 'ACTGA': 128122, 'ACTGT': 143545, 'ACTGC': 203225, 'ACTGG': 240791, 'ACCAA': 90997, 'ACCAT': 137010, 'ACCAC': 238461, 'ACCAG': 298529, 'ACCTA': 32223, 'ACCTT': 175568, 'ACCTC': 228234, 'ACCTG': 225705, 'ACCCA': 176332, 'ACCCT': 145867, 'ACCCC': 169171, 'ACCCG': 99572, 'ACCGA': 62718, 'ACCGT': 66876, 'ACCGC': 87005, 'ACCGG': 77374, 'ACGAA': 57163, 'ACGAT': 65046, 'ACGAC': 55450, 'ACGAG': 77678, 'ACGTA': 38623, 'ACGTT': 86697, 'ACGTC': 104325, 'ACGTG': 97909, 'ACGCA': 72581, 'ACGCT': 81646, 'ACGCC': 125636, 'ACGCG': 59695, 'ACGGA': 88778, 'ACGGT': 97530, 'ACGGC': 133292, 'ACGGG': 133277, 'AGAAA': 200564, 'AGAAT': 102244, 'AGAAC': 151542, 'AGAAG': 284790, 'AGATA': 84439, 'AGATT': 79126, 'AGATC': 123744, 'AGATG': 232952, 'AGACA': 149927, 'AGACT': 100925, 'AGACC': 163502, 'AGACG': 105850, 'AGAGA': 208675, 'AGAGT': 117457, 'AGAGC': 206059, 'AGAGG': 340836, 'AGTAA': 73097, 'AGTAT': 57130, 'AGTAC': 74576, 'AGTAG': 108205, 'AGTTA': 57897, 'AGTTT': 140458, 'AGTTC': 144710, 'AGTTG': 146337, 'AGTCA': 137703, 'AGTCT': 149051, 'AGTCC': 208459, 'AGTCG': 91232, 'AGTGA': 157342, 'AGTGT': 120831, 'AGTGC': 162294, 'AGTGG': 241314, 'AGCAA': 120742, 'AGCAT': 146179, 'AGCAC': 234638, 'AGCAG': 413027, 'AGCTA': 60552, 'AGCTT': 245855, 'AGCTC': 314374, 'AGCTG': 413135, 'AGCCA': 303325, 'AGCCT': 249502, 'AGCCC': 324152, 'AGCCG': 208201, 'AGCGA': 89474, 'AGCGT': 130712, 'AGCGC': 178119, 'AGCGG': 212402, 'AGGAA': 305626, 'AGGAT': 205075, 'AGGAC': 229425, 'AGGAG': 406814, 'AGGTA': 139280, 'AGGTT': 161058, 'AGGTC': 257076, 'AGGTG': 360546, 'AGGCA': 308880, 'AGGCT': 325011, 'AGGCC': 400171, 'AGGCG': 254066, 'AGGGA': 286989, 'AGGGT': 245673, 'AGGGC': 435744, 'AGGGG': 483892, 'TAAAA': 146869, 'TAAAT': 87212, 'TAAAC': 61422, 'TAAAG': 71002, 'TAATA': 49754, 'TAATT': 68117, 'TAATC': 62393, 'TAATG': 67831, 'TAACA': 67248, 'TAACT': 64256, 'TAACC': 40716, 'TAACG': 12274, 'TAAGA': 62276, 'TAAGT': 45461, 'TAAGC': 52857, 'TAAGG': 78109, 'TATAA': 46987, 'TATAT': 49965, 'TATAC': 30838, 'TATAG': 42890, 'TATTA': 36460, 'TATTT': 123020, 'TATTC': 67836, 'TATTG': 71681, 'TATCA': 58758, 'TATCT': 70440, 'TATCC': 58996, 'TATCG': 22641, 'TATGA': 48787, 'TATGT': 49091, 'TATGC': 35094, 'TATGG': 72474, 'TACAA': 80862, 'TACAT': 77030, 'TACAC': 80645, 'TACAG': 121186, 'TACTA': 21845, 'TACTT': 122454, 'TACTC': 89060, 'TACTG': 95923, 'TACCA': 67663, 'TACCT': 54187, 'TACCC': 59636, 'TACCG': 33128, 'TACGA': 19454, 'TACGT': 26584, 'TACGC': 21254, 'TACGG': 44182, 'TAGAA': 96924, 'TAGAT': 75512, 'TAGAC': 57876, 'TAGAG': 104590, 'TAGTA': 60185, 'TAGTT': 70323, 'TAGTC': 78110, 'TAGTG': 85260, 'TAGCA': 75593, 'TAGCT': 85627, 'TAGCC': 114978, 'TAGCG': 68542, 'TAGGA': 111203, 'TAGGT': 97382, 'TAGGC': 108705, 'TAGGG': 129485, 'TTAAA': 116174, 'TTAAT': 79378, 'TTAAC': 51704, 'TTAAG': 68664, 'TTATA': 48262, 'TTATT': 88683, 'TTATC': 58162, 'TTATG': 52582, 'TTACA': 86302, 'TTACT': 49088, 'TTACC': 42362, 'TTACG': 28179, 'TTAGA': 38376, 'TTAGT': 52091, 'TTAGC': 52631, 'TTAGG': 74649, 'TTTAA': 117298, 'TTTAT': 104297, 'TTTAC': 66974, 'TTTAG': 62204, 'TTTTA': 115720, 'TTTTT': 455236, 'TTTTC': 223829, 'TTTTG': 172046, 'TTTCA': 163990, 'TTTCT': 321018, 'TTTCC': 210767, 'TTTCG': 39584, 'TTTGA': 137559, 'TTTGT': 196967, 'TTTGC': 155706, 'TTTGG': 189613, 'TTCAA': 148576, 'TTCAT': 201972, 'TTCAC': 234949, 'TTCAG': 276966, 'TTCTA': 73887, 'TTCTT': 478320, 'TTCTC': 420752, 'TTCTG': 319638, 'TTCCA': 221918, 'TTCCT': 290991, 'TTCCC': 228172, 'TTCCG': 138423, 'TTCGA': 50027, 'TTCGT': 41219, 'TTCGC': 43711, 'TTCGG': 70342, 'TTGAA': 185580, 'TTGAT': 205264, 'TTGAC': 150465, 'TTGAG': 222548, 'TTGTA': 156626, 'TTGTT': 208211, 'TTGTC': 238430, 'TTGTG': 168281, 'TTGCA': 145865, 'TTGCT': 195426, 'TTGCC': 208004, 'TTGCG': 134722, 'TTGGA': 213655, 'TTGGT': 234022, 'TTGGC': 298305, 'TTGGG': 288533, 'TCAAA': 173449, 'TCAAT': 140792, 'TCAAC': 77640, 'TCAAG': 117903, 'TCATA': 102144, 'TCATT': 121289, 'TCATC': 317898, 'TCATG': 216052, 'TCACA': 209299, 'TCACT': 189549, 'TCACC': 226839, 'TCACG': 113641, 'TCAGA': 166296, 'TCAGT': 160196, 'TCAGC': 336129, 'TCAGG': 339103, 'TCTAA': 48412, 'TCTAT': 57377, 'TCTAC': 66504, 'TCTAG': 67468, 'TCTTA': 77297, 'TCTTT': 252606, 'TCTTC': 514755, 'TCTTG': 475043, 'TCTCA': 182770, 'TCTCT': 283202, 'TCTCC': 474467, 'TCTCG': 209971, 'TCTGA': 180644, 'TCTGT': 214815, 'TCTGC': 327967, 'TCTGG': 440501, 'TCCAA': 152889, 'TCCAT': 205867, 'TCCAC': 364421, 'TCCAG': 554722, 'TCCTA': 60698, 'TCCTT': 367917, 'TCCTC': 622344, 'TCCTG': 379616, 'TCCCA': 268994, 'TCCCT': 189979, 'TCCCC': 281614, 'TCCCG': 231495, 'TCCGA': 88593, 'TCCGT': 126903, 'TCCGC': 173385, 'TCCGG': 209081, 'TCGAA': 108206, 'TCGAT': 99797, 'TCGAC': 41949, 'TCGAG': 64185, 'TCGTA': 84292, 'TCGTT': 77023, 'TCGTC': 158814, 'TCGTG': 107794, 'TCGCA': 86295, 'TCGCT': 109622, 'TCGCC': 129813, 'TCGCG': 74144, 'TCGGA': 92309, 'TCGGT': 93854, 'TCGGC': 189884, 'TCGGG': 202793, 'TGAAA': 127027, 'TGAAT': 101593, 'TGAAC': 146910, 'TGAAG': 313570, 'TGATA': 64254, 'TGATT': 72364, 'TGATC': 165867, 'TGATG': 323163, 'TGACA': 153637, 'TGACT': 117256, 'TGACC': 186005, 'TGACG': 141214, 'TGAGA': 187001, 'TGAGT': 133462, 'TGAGC': 274943, 'TGAGG': 369662, 'TGTAA': 85356, 'TGTAT': 72008, 'TGTAC': 112267, 'TGTAG': 207112, 'TGTTA': 71210, 'TGTTT': 172777, 'TGTTC': 199117, 'TGTTG': 278522, 'TGTCA': 194892, 'TGTCT': 223092, 'TGTCC': 328717, 'TGTCG': 129233, 'TGTGA': 143467, 'TGTGT': 179427, 'TGTGC': 190076, 'TGTGG': 304565, 'TGCAA': 114596, 'TGCAT': 145600, 'TGCAC': 194867, 'TGCAG': 380922, 'TGCTA': 52780, 'TGCTT': 227783, 'TGCTC': 298046, 'TGCTG': 469820, 'TGCCA': 256738, 'TGCCT': 240545, 'TGCCC': 316120, 'TGCCG': 226793, 'TGCGA': 54065, 'TGCGT': 89798, 'TGCGC': 158157, 'TGCGG': 256783, 'TGGAA': 247326, 'TGGAT': 177021, 'TGGAC': 203399, 'TGGAG': 408229, 'TGGTA': 127877, 'TGGTT': 186795, 'TGGTC': 318364, 'TGGTG': 398336, 'TGGCA': 313645, 'TGGCT': 342638, 'TGGCC': 483177, 'TGGCG': 254415, 'TGGGA': 300470, 'TGGGT': 295744, 'TGGGC': 441986, 'TGGGG': 622653, 'CAAAA': 188137, 'CAAAT': 112838, 'CAAAC': 169773, 'CAAAG': 215372, 'CAATA': 61446, 'CAATT': 77211, 'CAATC': 100143, 'CAATG': 165422, 'CAACA': 128925, 'CAACT': 94595, 'CAACC': 93969, 'CAACG': 49107, 'CAAGA': 116513, 'CAAGT': 122805, 'CAAGC': 115734, 'CAAGG': 202941, 'CATAA': 70681, 'CATAT': 54971, 'CATAC': 93826, 'CATAG': 139599, 'CATTA': 65382, 'CATTT': 128420, 'CATTC': 152509, 'CATTG': 130596, 'CATCA': 235247, 'CATCT': 297324, 'CATCC': 251346, 'CATCG': 91730, 'CATGA': 211992, 'CATGT': 184862, 'CATGC': 178860, 'CATGG': 279279, 'CACAA': 182712, 'CACAT': 187794, 'CACAC': 230214, 'CACAG': 352252, 'CACTA': 50932, 'CACTT': 165832, 'CACTC': 183957, 'CACTG': 323229, 'CACCA': 380749, 'CACCT': 329938, 'CACCC': 239657, 'CACCG': 130762, 'CACGA': 122435, 'CACGT': 166467, 'CACGC': 169119, 'CACGG': 210359, 'CAGAA': 238405, 'CAGAT': 169199, 'CAGAC': 181525, 'CAGAG': 322762, 'CAGTA': 95258, 'CAGTT': 165362, 'CAGTC': 184912, 'CAGTG': 262908, 'CAGCA': 479072, 'CAGCT': 519481, 'CAGCC': 470577, 'CAGCG': 281844, 'CAGGA': 475175, 'CAGGT': 395803, 'CAGGC': 520658, 'CAGGG': 628561, 'CTAAA': 53089, 'CTAAT': 34989, 'CTAAC': 40369, 'CTAAG': 53009, 'CTATA': 24168, 'CTATT': 54877, 'CTATC': 47836, 'CTATG': 47961, 'CTACA': 62048, 'CTACT': 52174, 'CTACC': 50437, 'CTACG': 27740, 'CTAGA': 62751, 'CTAGT': 25862, 'CTAGC': 50365, 'CTAGG': 84278, 'CTTAA': 74266, 'CTTAT': 46625, 'CTTAC': 35452, 'CTTAG': 68116, 'CTTTA': 116562, 'CTTTT': 196660, 'CTTTC': 198336, 'CTTTG': 226745, 'CTTCA': 400396, 'CTTCT': 572472, 'CTTCC': 388477, 'CTTCG': 77879, 'CTTGA': 338134, 'CTTGT': 304714, 'CTTGC': 308271, 'CTTGG': 473778, 'CTCAA': 152008, 'CTCAT': 233041, 'CTCAC': 173287, 'CTCAG': 334545, 'CTCTA': 75897, 'CTCTT': 302349, 'CTCTC': 258806, 'CTCTG': 375709, 'CTCCA': 549975, 'CTCCT': 624207, 'CTCCC': 327996, 'CTCCG': 240131, 'CTCGA': 140196, 'CTCGT': 209454, 'CTCGC': 202426, 'CTCGG': 289472, 'CTGAA': 170332, 'CTGAT': 113009, 'CTGAC': 163614, 'CTGAG': 297917, 'CTGTA': 124122, 'CTGTT': 177814, 'CTGTC': 268382, 'CTGTG': 307731, 'CTGCA': 387026, 'CTGCT': 461280, 'CTGCC': 424654, 'CTGCG': 183954, 'CTGGA': 379965, 'CTGGT': 357859, 'CTGGC': 444341, 'CTGGG': 687859, 'CCAAA': 190381, 'CCAAT': 75983, 'CCAAC': 120086, 'CCAAG': 184137, 'CCATA': 95676, 'CCATT': 134699, 'CCATC': 229600, 'CCATG': 296899, 'CCACA': 325202, 'CCACT': 223220, 'CCACC': 438890, 'CCACG': 281742, 'CCAGA': 291270, 'CCAGT': 221918, 'CCAGC': 598483, 'CCAGG': 753662, 'CCTAA': 41811, 'CCTAT': 47908, 'CCTAC': 38935, 'CCTAG': 59657, 'CCTTA': 51934, 'CCTTT': 165873, 'CCTTC': 374491, 'CCTTG': 418674, 'CCTCA': 312510, 'CCTCT': 338179, 'CCTCC': 622436, 'CCTCG': 303550, 'CCTGA': 196943, 'CCTGT': 208317, 'CCTGC': 389415, 'CCTGG': 547603, 'CCCAA': 161800, 'CCCAT': 198522, 'CCCAC': 311428, 'CCCAG': 529486, 'CCCTA': 50168, 'CCCTT': 217701, 'CCCTC': 303494, 'CCCTG': 335788, 'CCCCA': 361787, 'CCCCT': 241738, 'CCCCC': 301384, 'CCCCG': 267173, 'CCCGA': 135401, 'CCCGT': 146495, 'CCCGC': 245537, 'CCCGG': 345582, 'CCGAA': 84290, 'CCGAT': 78413, 'CCGAC': 83995, 'CCGAG': 171587, 'CCGTA': 71836, 'CCGTT': 100364, 'CCGTC': 173447, 'CCGTG': 180446, 'CCGCA': 170933, 'CCGCT': 229522, 'CCGCC': 306561, 'CCGCG': 161194, 'CCGGA': 160118, 'CCGGT': 161403, 'CCGGC': 291784, 'CCGGG': 351258, 'CGAAA': 45027, 'CGAAT': 41943, 'CGAAC': 65732, 'CGAAG': 153252, 'CGATA': 20883, 'CGATT': 31789, 'CGATC': 85430, 'CGATG': 185621, 'CGACA': 59349, 'CGACT': 65220, 'CGACC': 75784, 'CGACG': 51703, 'CGAGA': 86566, 'CGAGT': 69325, 'CGAGC': 106849, 'CGAGG': 186385, 'CGTAA': 21913, 'CGTAT': 25861, 'CGTAC': 54306, 'CGTAG': 140965, 'CGTTA': 18971, 'CGTTT': 74798, 'CGTTC': 121119, 'CGTTG': 137231, 'CGTCA': 108051, 'CGTCT': 151484, 'CGTCC': 202126, 'CGTCG': 130557, 'CGTGA': 97549, 'CGTGT': 104238, 'CGTGC': 143710, 'CGTGG': 228886, 'CGCAA': 40560, 'CGCAT': 68282, 'CGCAC': 126254, 'CGCAG': 266352, 'CGCTA': 19450, 'CGCTT': 127601, 'CGCTC': 216455, 'CGCTG': 247162, 'CGCCA': 176937, 'CGCCT': 175139, 'CGCCC': 247961, 'CGCCG': 203972, 'CGCGA': 57260, 'CGCGT': 66022, 'CGCGC': 126453, 'CGCGG': 193022, 'CGGAA': 104147, 'CGGAT': 130447, 'CGGAC': 106858, 'CGGAG': 186334, 'CGGTA': 92895, 'CGGTT': 86829, 'CGGTC': 138301, 'CGGTG': 217004, 'CGGCA': 181388, 'CGGCT': 211724, 'CGGCC': 353143, 'CGGCG': 243133, 'CGGGA': 192132, 'CGGGT': 134467, 'CGGGC': 338263, 'CGGGG': 361020, 'GAAAA': 149910, 'GAAAT': 130046, 'GAAAC': 116223, 'GAAAG': 164367, 'GAATA': 62555, 'GAATT': 92172, 'GAATC': 101673, 'GAATG': 114279, 'GAACA': 155880, 'GAACT': 211185, 'GAACC': 119457, 'GAACG': 53117, 'GAAGA': 268706, 'GAAGT': 223171, 'GAAGC': 285901, 'GAAGG': 385753, 'GATAA': 56328, 'GATAT': 49550, 'GATAC': 52772, 'GATAG': 66736, 'GATTA': 35762, 'GATTT': 100910, 'GATTC': 83152, 'GATTG': 62042, 'GATCA': 144721, 'GATCT': 262809, 'GATCC': 133118, 'GATCG': 37256, 'GATGA': 283718, 'GATGT': 261955, 'GATGC': 214119, 'GATGG': 331654, 'GACAA': 105036, 'GACAT': 122141, 'GACAC': 150082, 'GACAG': 238729, 'GACTA': 32940, 'GACTT': 146106, 'GACTC': 158403, 'GACTG': 137871, 'GACCA': 205326, 'GACCT': 175289, 'GACCC': 178561, 'GACCG': 86017, 'GACGA': 88129, 'GACGT': 99148, 'GACGC': 106373, 'GACGG': 142063, 'GAGAA': 227590, 'GAGAT': 143081, 'GAGAC': 171622, 'GAGAG': 260391, 'GAGTA': 68045, 'GAGTT': 122062, 'GAGTC': 176666, 'GAGTG': 170344, 'GAGCA': 220658, 'GAGCT': 282456, 'GAGCC': 286972, 'GAGCG': 156865, 'GAGGA': 370298, 'GAGGT': 264439, 'GAGGC': 407149, 'GAGGG': 448417, 'GTAAA': 67782, 'GTAAT': 73447, 'GTAAC': 48010, 'GTAAG': 56990, 'GTATA': 38313, 'GTATT': 79409, 'GTATC': 55255, 'GTATG': 55144, 'GTACA': 122337, 'GTACT': 144250, 'GTACC': 73304, 'GTACG': 37189, 'GTAGA': 158848, 'GTAGT': 143841, 'GTAGC': 157634, 'GTAGG': 199891, 'GTTAA': 59940, 'GTTAT': 49489, 'GTTAC': 47824, 'GTTAG': 43111, 'GTTTA': 51846, 'GTTTT': 157079, 'GTTTC': 178277, 'GTTTG': 148997, 'GTTCA': 187022, 'GTTCT': 260526, 'GTTCC': 162035, 'GTTCG': 46587, 'GTTGA': 202822, 'GTTGT': 196906, 'GTTGC': 141049, 'GTTGG': 267627, 'GTCAA': 120540, 'GTCAT': 160439, 'GTCAC': 210958, 'GTCAG': 223981, 'GTCTA': 51162, 'GTCTT': 246384, 'GTCTC': 245680, 'GTCTG': 246742, 'GTCCA': 319197, 'GTCCT': 342276, 'GTCCC': 296792, 'GTCCG': 142374, 'GTCGA': 87309, 'GTCGT': 126617, 'GTCGC': 102567, 'GTCGG': 167091, 'GTGAA': 168687, 'GTGAT': 134451, 'GTGAC': 160926, 'GTGAG': 240775, 'GTGTA': 93451, 'GTGTT': 144030, 'GTGTC': 181309, 'GTGTG': 206688, 'GTGCA': 197069, 'GTGCT': 258740, 'GTGCC': 229429, 'GTGCG': 119893, 'GTGGA': 283999, 'GTGGT': 237673, 'GTGGC': 389164, 'GTGGG': 464016, 'GCAAA': 143183, 'GCAAT': 84634, 'GCAAC': 90637, 'GCAAG': 131429, 'GCATA': 81453, 'GCATT': 96785, 'GCATC': 188394, 'GCATG': 192692, 'GCACA': 228766, 'GCACT': 198521, 'GCACC': 239442, 'GCACG': 166955, 'GCAGA': 270522, 'GCAGT': 194120, 'GCAGC': 552545, 'GCAGG': 647172, 'GCTAA': 51289, 'GCTAT': 32548, 'GCTAC': 55293, 'GCTAG': 58297, 'GCTTA': 56918, 'GCTTT': 175919, 'GCTTC': 349667, 'GCTTG': 307949, 'GCTCA': 265460, 'GCTCT': 265068, 'GCTCC': 461973, 'GCTCG': 204476, 'GCTGA': 240879, 'GCTGT': 310615, 'GCTGC': 539529, 'GCTGG': 642860, 'GCCAA': 165662, 'GCCAT': 214850, 'GCCAC': 358098, 'GCCAG': 482424, 'GCCTA': 45386, 'GCCTT': 250354, 'GCCTC': 421360, 'GCCTG': 400640, 'GCCCA': 397569, 'GCCCT': 331467, 'GCCCC': 426218, 'GCCCG': 277856, 'GCCGA': 132999, 'GCCGT': 185411, 'GCCGC': 363963, 'GCCGG': 333167, 'GCGAA': 57162, 'GCGAT': 80464, 'GCGAC': 72044, 'GCGAG': 136971, 'GCGTA': 48118, 'GCGTT': 86662, 'GCGTC': 157791, 'GCGTG': 188174, 'GCGCA': 171677, 'GCGCT': 192914, 'GCGCC': 245238, 'GCGCG': 150034, 'GCGGA': 186866, 'GCGGT': 183923, 'GCGGC': 377563, 'GCGGG': 339790, 'GGAAA': 186726, 'GGAAT': 125534, 'GGAAC': 176577, 'GGAAG': 413375, 'GGATA': 55724, 'GGATT': 96961, 'GGATC': 201966, 'GGATG': 349989, 'GGACA': 251533, 'GGACT': 194086, 'GGACC': 220719, 'GGACG': 137388, 'GGAGA': 322048, 'GGAGT': 217526, 'GGAGC': 361194, 'GGAGG': 592241, 'GGTAA': 65419, 'GGTAT': 71936, 'GGTAC': 136018, 'GGTAG': 203974, 'GGTTA': 50914, 'GGTTT': 147748, 'GGTTC': 193023, 'GGTTG': 244552, 'GGTCA': 273155, 'GGTCT': 268112, 'GGTCC': 358771, 'GGTCG': 131803, 'GGTGA': 304166, 'GGTGT': 217701, 'GGTGC': 307865, 'GGTGG': 597580, 'GGCAA': 173957, 'GGCAT': 199423, 'GGCAC': 281401, 'GGCAG': 608458, 'GGCTA': 65021, 'GGCTT': 292232, 'GGCTC': 371348, 'GGCTG': 601250, 'GGCCA': 489351, 'GGCCT': 455760, 'GGCCC': 546659, 'GGCCG': 380437, 'GGCGA': 145667, 'GGCGT': 195284, 'GGCGC': 297948, 'GGCGG': 426807, 'GGGAA': 245082, 'GGGAT': 189904, 'GGGAC': 265924, 'GGGAG': 493071, 'GGGTA': 117081, 'GGGTT': 199310, 'GGGTC': 321969, 'GGGTG': 444429, 'GGGCA': 458898, 'GGGCT': 451205, 'GGGCC': 640777, 'GGGCG': 314912, 'GGGGA': 412260, 'GGGGT': 405590, 'GGGGC': 651192, 'GGGGG': 588986}, 'overrepresented_sequences': {}} {'total_reads': 3511866, 'total_bases': 221225441, 'q20_bases': 213770455, 'q30_bases': 206355536, 'total_cycles': 63, 'quality_curves': {'A': [37.1811, 37.1901, 37.1833, 37.224, 37.1979, 37.2092, 37.1818, 37.19, 37.1045, 37.0583, 37.1225, 37.1218, 37.0875, 37.0462, 36.9698, 36.9723, 36.9605, 36.915, 36.936, 36.958, 36.97, 36.9532, 36.9311, 36.8549, 36.7491, 36.6024, 36.522, 36.4666, 36.3661, 36.3582, 36.2921, 36.2664, 36.2146, 36.0301, 36.027, 36.0007, 35.9304, 35.9344, 35.854, 35.798, 35.7192, 35.4331, 35.6366, 35.5461, 35.6213, 35.5925, 35.5471, 35.4696, 35.5201, 35.4407, 35.3144, 35.1944, 35.1804, 35.0829, 34.942, 34.9129, 34.8517, 34.6979, 34.6642, 34.3759, 33.8339, 33.4551, 24.5872], 'T': [37.5027, 37.48, 37.4817, 37.4753, 37.4459, 37.4395, 37.4007, 37.4051, 37.3192, 37.2897, 37.2948, 37.3, 37.2984, 37.2681, 37.2597, 37.2728, 37.2743, 37.2156, 37.2125, 37.2129, 37.235, 37.2124, 37.1962, 37.1193, 37.0556, 36.9338, 36.8474, 36.8217, 36.7328, 36.7021, 36.6808, 36.6485, 36.5768, 36.4575, 36.445, 36.3936, 36.3801, 36.3318, 36.247, 36.1837, 36.1177, 35.8439, 36.0124, 35.9484, 35.9563, 35.9773, 35.9238, 35.8924, 35.9118, 35.8222, 35.6769, 35.584, 35.5239, 35.4687, 35.3548, 35.296, 35.2673, 35.1157, 35.0619, 34.7747, 34.2226, 33.9669, 25.3372], 'C': [37.3433, 37.3699, 37.329, 37.3307, 37.3429, 37.3316, 37.3456, 37.339, 37.2631, 37.2763, 37.2901, 37.3088, 37.2768, 37.3009, 37.2372, 37.223, 37.2479, 37.2399, 37.2315, 37.2277, 37.2132, 37.215, 37.191, 37.1434, 37.0585, 36.9973, 36.9073, 36.8429, 36.8047, 36.7406, 36.7244, 36.7262, 36.6557, 36.5811, 36.5686, 36.5141, 36.5058, 36.4821, 36.425, 36.3794, 36.3558, 36.0726, 36.2218, 36.2571, 36.2426, 36.2311, 36.2364, 36.1866, 36.1767, 36.1428, 36.0371, 35.9517, 35.8877, 35.8005, 35.7447, 35.6487, 35.6258, 35.4621, 35.3823, 35.197, 34.6267, 34.2958, 30.2382], 'G': [37.122, 37.154, 37.1237, 37.1588, 37.1299, 37.122, 37.1218, 37.147, 37.0551, 37.0634, 37.0971, 37.101, 37.0501, 37.0383, 36.9719, 36.9551, 36.9611, 36.9467, 36.9525, 36.9861, 36.9696, 36.965, 36.9359, 36.8496, 36.775, 36.6776, 36.5854, 36.5451, 36.4812, 36.4203, 36.4203, 36.4115, 36.3406, 36.2436, 36.2201, 36.1974, 36.1856, 36.1718, 36.0826, 36.0587, 35.986, 35.7526, 35.9125, 35.8778, 35.8928, 35.9166, 35.8677, 35.7763, 35.8081, 35.7662, 35.6347, 35.5298, 35.4886, 35.4203, 35.3316, 35.2768, 35.2013, 35.0773, 34.9983, 34.7444, 34.1312, 33.7724, 28.717], 'mean': [37.2857, 37.2952, 37.2745, 37.2901, 37.2726, 37.269, 37.2575, 37.2669, 37.1822, 37.1728, 37.2, 37.2075, 37.1761, 37.1636, 37.109, 37.1035, 37.11, 37.0817, 37.0856, 37.0987, 37.0964, 37.0872, 37.0642, 36.9925, 36.9124, 36.8111, 36.7209, 36.6754, 36.6062, 36.56, 36.5391, 36.5251, 36.4566, 36.3444, 36.3324, 36.2907, 36.2696, 36.2491, 36.1692, 36.1256, 36.0681, 35.7983, 35.9677, 35.9382, 35.9518, 35.9541, 35.9233, 35.8565, 35.8789, 35.8215, 35.6937, 35.5955, 35.551, 35.4712, 35.3773, 35.3163, 35.2659, 35.1216, 35.0578, 34.8051, 34.2337, 33.9016, 27.5741]}, 'content_curves': {'A': [0.199996, 0.19328, 0.20078, 0.200931, 0.196628, 0.20369, 0.19898, 0.195166, 0.204191, 0.197045, 0.199189, 0.204411, 0.202344, 0.196442, 0.204974, 0.200619, 0.197748, 0.203384, 0.199234, 0.195308, 0.202705, 0.198803, 0.195594, 0.200471, 0.195605, 0.193887, 0.200557, 0.194672, 0.193531, 0.198723, 0.194674, 0.193492, 0.1995, 0.196531, 0.19288, 0.200173, 0.196718, 0.194606, 0.201646, 0.196183, 0.194744, 0.199898, 0.196273, 0.192265, 0.198128, 0.197692, 0.19293, 0.198989, 0.193848, 0.194445, 0.197724, 0.19383, 0.194578, 0.199167, 0.196601, 0.195055, 0.198851, 0.195372, 0.193334, 0.199617, 0.195114, 0.192807, 0.195954], 'T': [0.247855, 0.230821, 0.237331, 0.228727, 0.223756, 0.231603, 0.227054, 0.226168, 0.231617, 0.232504, 0.223243, 0.229069, 0.229644, 0.220001, 0.225374, 0.225262, 0.219505, 0.225532, 0.227137, 0.222058, 0.22475, 0.226472, 0.221076, 0.225583, 0.225071, 0.221369, 0.225854, 0.226972, 0.222665, 0.227319, 0.226935, 0.221531, 0.228192, 0.229154, 0.22438, 0.228159, 0.226939, 0.222025, 0.222998, 0.225403, 0.220871, 0.226749, 0.226533, 0.223448, 0.226068, 0.228236, 0.222238, 0.226991, 0.226679, 0.222805, 0.222113, 0.226828, 0.217959, 0.221091, 0.222661, 0.217107, 0.222613, 0.219211, 0.216496, 0.219596, 0.223606, 0.21594, 0.214871], 'C': [0.260174, 0.272954, 0.263306, 0.266507, 0.275474, 0.265815, 0.27, 0.276555, 0.268674, 0.271489, 0.278354, 0.272926, 0.271112, 0.278565, 0.275042, 0.273739, 0.27966, 0.275499, 0.277107, 0.280362, 0.275078, 0.274185, 0.280942, 0.275479, 0.279963, 0.285604, 0.276732, 0.277984, 0.282253, 0.274668, 0.278379, 0.283229, 0.277055, 0.277892, 0.284111, 0.277408, 0.281299, 0.283386, 0.280247, 0.28036, 0.284209, 0.277706, 0.280291, 0.285689, 0.281596, 0.279197, 0.284787, 0.280397, 0.279672, 0.281692, 0.281949, 0.280688, 0.2872, 0.282606, 0.283437, 0.285806, 0.28124, 0.286072, 0.287326, 0.282062, 0.282931, 0.286048, 0.286431], 'G': [0.291976, 0.302945, 0.298579, 0.303835, 0.304142, 0.298893, 0.303966, 0.302111, 0.295518, 0.298963, 0.299213, 0.293593, 0.2969, 0.304991, 0.2946, 0.30038, 0.303086, 0.295585, 0.296522, 0.302272, 0.297467, 0.300539, 0.302388, 0.298467, 0.299361, 0.29914, 0.296857, 0.300373, 0.30155, 0.29929, 0.300012, 0.301748, 0.295253, 0.296424, 0.298629, 0.29426, 0.295043, 0.299983, 0.295108, 0.298054, 0.300173, 0.295646, 0.296903, 0.298598, 0.294205, 0.294872, 0.300045, 0.293618, 0.299802, 0.301058, 0.298198, 0.298653, 0.300263, 0.297136, 0.297301, 0.302033, 0.297295, 0.299343, 0.302843, 0.298723, 0.298345, 0.305163, 0.301129], 'N': [0, 0, 4.55598e-06, 0, 0, 0, 0, 0, 0, 0, 1.139e-06, 1.70849e-06, 0, 0, 9.68146e-06, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 2.84761e-06, 1.13905e-06, 0, 0, 3.41722e-06, 3.70203e-06, 0, 3.70214e-06, 0, 0, 1.50943e-05, 0, 0, 0, 0, 0, 0, 1.42446e-06, 0, 1.99425e-06, 3.7036e-06, 4.10245e-05, 0.00161392], 'GC': [0.55215, 0.5759, 0.561884, 0.570342, 0.579616, 0.564707, 0.573966, 0.578667, 0.564192, 0.570451, 0.577567, 0.566518, 0.568012, 0.583557, 0.569642, 0.574119, 0.582747, 0.571084, 0.573629, 0.582634, 0.572545, 0.574724, 0.58333, 0.573946, 0.579324, 0.584744, 0.573589, 0.578357, 0.583803, 0.573958, 0.578391, 0.584977, 0.572308, 0.574315, 0.58274, 0.571668, 0.576343, 0.583369, 0.575355, 0.578414, 0.584382, 0.573352, 0.577194, 0.584287, 0.575801, 0.574068, 0.584831, 0.574015, 0.579473, 0.58275, 0.580148, 0.579342, 0.587463, 0.579742, 0.580738, 0.587839, 0.578535, 0.585416, 0.59017, 0.580785, 0.581276, 0.591212, 0.587561]}, 'kmer_count': {'AAAAA': 421738, 'AAAAT': 197150, 'AAAAC': 152467, 'AAAAG': 197187, 'AAATA': 165353, 'AAATT': 134034, 'AAATC': 123270, 'AAATG': 142441, 'AAACA': 184682, 'AAACT': 164299, 'AAACC': 135348, 'AAACG': 52681, 'AAAGA': 177936, 'AAAGT': 152767, 'AAAGC': 174669, 'AAAGG': 195375, 'AATAA': 128704, 'AATAT': 82065, 'AATAC': 71513, 'AATAG': 78043, 'AATTA': 72319, 'AATTT': 140467, 'AATTC': 111367, 'AATTG': 73635, 'AATCA': 101022, 'AATCT': 153776, 'AATCC': 118881, 'AATCG': 45316, 'AATGA': 121999, 'AATGT': 119928, 'AATGC': 117992, 'AATGG': 168012, 'AACAA': 127615, 'AACAT': 122644, 'AACAC': 145570, 'AACAG': 180024, 'AACTA': 44919, 'AACTT': 188621, 'AACTC': 163026, 'AACTG': 177115, 'AACCA': 122768, 'AACCT': 115844, 'AACCC': 129529, 'AACCG': 48700, 'AACGA': 29111, 'AACGT': 39527, 'AACGC': 48469, 'AACGG': 62545, 'AAGAA': 196150, 'AAGAT': 147385, 'AAGAC': 125599, 'AAGAG': 206286, 'AAGTA': 98248, 'AAGTT': 148858, 'AAGTC': 162489, 'AAGTG': 177031, 'AAGCA': 156295, 'AAGCT': 167382, 'AAGCC': 237684, 'AAGCG': 116271, 'AAGGA': 208394, 'AAGGT': 176335, 'AAGGC': 282841, 'AAGGG': 275512, 'ATAAA': 143259, 'ATAAT': 66725, 'ATAAC': 48149, 'ATAAG': 65630, 'ATATA': 66831, 'ATATT': 79472, 'ATATC': 53944, 'ATATG': 54082, 'ATACA': 98998, 'ATACT': 94269, 'ATACC': 55941, 'ATACG': 21393, 'ATAGA': 84463, 'ATAGT': 78358, 'ATAGC': 93596, 'ATAGG': 98637, 'ATTAA': 69645, 'ATTAT': 49672, 'ATTAC': 60499, 'ATTAG': 47335, 'ATTTA': 71036, 'ATTTT': 174089, 'ATTTC': 147794, 'ATTTG': 141399, 'ATTCA': 121068, 'ATTCT': 150191, 'ATTCC': 131088, 'ATTCG': 46832, 'ATTGA': 90993, 'ATTGT': 77784, 'ATTGC': 85600, 'ATTGG': 112822, 'ATCAA': 97213, 'ATCAT': 178666, 'ATCAC': 130232, 'ATCAG': 181454, 'ATCTA': 41567, 'ATCTT': 318166, 'ATCTC': 248850, 'ATCTG': 241855, 'ATCCA': 202887, 'ATCCT': 189643, 'ATCCC': 135902, 'ATCCG': 85098, 'ATCGA': 41348, 'ATCGT': 56954, 'ATCGC': 58994, 'ATCGG': 58805, 'ATGAA': 178670, 'ATGAT': 187656, 'ATGAC': 136302, 'ATGAG': 222943, 'ATGTA': 110728, 'ATGTT': 211337, 'ATGTC': 205372, 'ATGTG': 144051, 'ATGCA': 118950, 'ATGCT': 146926, 'ATGCC': 198070, 'ATGCG': 131316, 'ATGGA': 173056, 'ATGGT': 219240, 'ATGGC': 296015, 'ATGGG': 244426, 'ACAAA': 199881, 'ACAAT': 112864, 'ACAAC': 87153, 'ACAAG': 136138, 'ACATA': 92632, 'ACATT': 137858, 'ACATC': 157145, 'ACATG': 167574, 'ACACA': 214040, 'ACACT': 124752, 'ACACC': 199216, 'ACACG': 120287, 'ACAGA': 209164, 'ACAGT': 149355, 'ACAGC': 305442, 'ACAGG': 312074, 'ACTAA': 43724, 'ACTAT': 41081, 'ACTAC': 36597, 'ACTAG': 42007, 'ACTTA': 42787, 'ACTTT': 158084, 'ACTTC': 224577, 'ACTTG': 244755, 'ACTCA': 150497, 'ACTCT': 142444, 'ACTCC': 212986, 'ACTCG': 142591, 'ACTGA': 142724, 'ACTGT': 158849, 'ACTGC': 227338, 'ACTGG': 267993, 'ACCAA': 100069, 'ACCAT': 152098, 'ACCAC': 264909, 'ACCAG': 332328, 'ACCTA': 35089, 'ACCTT': 194997, 'ACCTC': 254111, 'ACCTG': 250316, 'ACCCA': 196431, 'ACCCT': 165235, 'ACCCC': 192859, 'ACCCG': 113285, 'ACCGA': 68671, 'ACCGT': 73939, 'ACCGC': 96506, 'ACCGG': 88003, 'ACGAA': 65446, 'ACGAT': 72880, 'ACGAC': 62809, 'ACGAG': 89746, 'ACGTA': 42685, 'ACGTT': 97305, 'ACGTC': 116357, 'ACGTG': 108203, 'ACGCA': 83493, 'ACGCT': 90522, 'ACGCC': 140431, 'ACGCG': 68231, 'ACGGA': 98673, 'ACGGT': 107596, 'ACGGC': 148990, 'ACGGG': 148473, 'AGAAA': 223290, 'AGAAT': 113210, 'AGAAC': 168676, 'AGAAG': 317215, 'AGATA': 94215, 'AGATT': 88575, 'AGATC': 139422, 'AGATG': 260875, 'AGACA': 168804, 'AGACT': 113887, 'AGACC': 185446, 'AGACG': 120562, 'AGAGA': 234573, 'AGAGT': 130582, 'AGAGC': 231870, 'AGAGG': 380322, 'AGTAA': 80812, 'AGTAT': 63453, 'AGTAC': 83633, 'AGTAG': 120578, 'AGTTA': 63972, 'AGTTT': 154583, 'AGTTC': 162946, 'AGTTG': 163875, 'AGTCA': 151123, 'AGTCT': 165486, 'AGTCC': 232299, 'AGTCG': 102667, 'AGTGA': 173912, 'AGTGT': 132499, 'AGTGC': 180225, 'AGTGG': 271777, 'AGCAA': 134558, 'AGCAT': 163032, 'AGCAC': 262605, 'AGCAG': 463726, 'AGCTA': 70142, 'AGCTT': 275532, 'AGCTC': 353519, 'AGCTG': 462699, 'AGCCA': 338887, 'AGCCT': 277549, 'AGCCC': 363170, 'AGCCG': 233055, 'AGCGA': 99859, 'AGCGT': 143893, 'AGCGC': 200978, 'AGCGG': 239640, 'AGGAA': 337472, 'AGGAT': 226981, 'AGGAC': 255731, 'AGGAG': 456173, 'AGGTA': 155649, 'AGGTT': 178550, 'AGGTC': 285450, 'AGGTG': 401805, 'AGGCA': 346623, 'AGGCT': 363911, 'AGGCC': 449160, 'AGGCG': 283158, 'AGGGA': 321704, 'AGGGT': 278437, 'AGGGC': 487301, 'AGGGG': 548211, 'TAAAA': 167381, 'TAAAT': 97376, 'TAAAC': 67603, 'TAAAG': 78846, 'TAATA': 56025, 'TAATT': 75246, 'TAATC': 68934, 'TAATG': 74119, 'TAACA': 73774, 'TAACT': 70323, 'TAACC': 45246, 'TAACG': 13550, 'TAAGA': 68775, 'TAAGT': 49898, 'TAAGC': 58210, 'TAAGG': 86196, 'TATAA': 53224, 'TATAT': 55815, 'TATAC': 34011, 'TATAG': 47431, 'TATTA': 41059, 'TATTT': 136320, 'TATTC': 74142, 'TATTG': 77816, 'TATCA': 65198, 'TATCT': 76199, 'TATCC': 65385, 'TATCG': 26025, 'TATGA': 53604, 'TATGT': 55512, 'TATGC': 39217, 'TATGG': 79863, 'TACAA': 89439, 'TACAT': 85935, 'TACAC': 88961, 'TACAG': 132975, 'TACTA': 24720, 'TACTT': 136726, 'TACTC': 99120, 'TACTG': 107862, 'TACCA': 74922, 'TACCT': 61282, 'TACCC': 67617, 'TACCG': 36286, 'TACGA': 22122, 'TACGT': 30416, 'TACGC': 23734, 'TACGG': 49676, 'TAGAA': 107002, 'TAGAT': 84914, 'TAGAC': 64731, 'TAGAG': 116039, 'TAGTA': 67682, 'TAGTT': 77695, 'TAGTC': 87057, 'TAGTG': 95330, 'TAGCA': 83960, 'TAGCT': 95932, 'TAGCC': 128455, 'TAGCG': 76041, 'TAGGA': 123404, 'TAGGT': 107586, 'TAGGC': 125079, 'TAGGG': 145893, 'TTAAA': 132687, 'TTAAT': 87964, 'TTAAC': 56977, 'TTAAG': 75228, 'TTATA': 54491, 'TTATT': 99730, 'TTATC': 64917, 'TTATG': 57305, 'TTACA': 95206, 'TTACT': 55142, 'TTACC': 46408, 'TTACG': 31100, 'TTAGA': 43615, 'TTAGT': 58185, 'TTAGC': 58198, 'TTAGG': 83146, 'TTTAA': 132083, 'TTTAT': 117145, 'TTTAC': 73920, 'TTTAG': 70938, 'TTTTA': 134458, 'TTTTT': 635638, 'TTTTC': 249510, 'TTTTG': 195621, 'TTTCA': 182195, 'TTTCT': 357013, 'TTTCC': 231747, 'TTTCG': 45269, 'TTTGA': 152879, 'TTTGT': 218548, 'TTTGC': 173711, 'TTTGG': 211299, 'TTCAA': 162676, 'TTCAT': 224765, 'TTCAC': 260839, 'TTCAG': 310016, 'TTCTA': 80120, 'TTCTT': 531124, 'TTCTC': 468317, 'TTCTG': 356659, 'TTCCA': 243934, 'TTCCT': 323244, 'TTCCC': 253216, 'TTCCG': 158909, 'TTCGA': 55423, 'TTCGT': 46720, 'TTCGC': 49214, 'TTCGG': 80073, 'TTGAA': 207039, 'TTGAT': 223933, 'TTGAC': 164017, 'TTGAG': 248363, 'TTGTA': 175419, 'TTGTT': 232058, 'TTGTC': 263578, 'TTGTG': 186159, 'TTGCA': 161488, 'TTGCT': 215888, 'TTGCC': 232004, 'TTGCG': 150984, 'TTGGA': 238081, 'TTGGT': 260804, 'TTGGC': 330253, 'TTGGG': 322123, 'TCAAA': 192676, 'TCAAT': 154930, 'TCAAC': 86123, 'TCAAG': 130590, 'TCATA': 113590, 'TCATT': 134242, 'TCATC': 350948, 'TCATG': 240967, 'TCACA': 233814, 'TCACT': 208655, 'TCACC': 253635, 'TCACG': 125720, 'TCAGA': 185727, 'TCAGT': 177828, 'TCAGC': 377493, 'TCAGG': 380145, 'TCTAA': 52520, 'TCTAT': 62055, 'TCTAC': 74621, 'TCTAG': 74200, 'TCTTA': 86543, 'TCTTT': 283507, 'TCTTC': 569868, 'TCTTG': 524875, 'TCTCA': 203555, 'TCTCT': 315704, 'TCTCC': 528577, 'TCTCG': 234578, 'TCTGA': 201018, 'TCTGT': 238941, 'TCTGC': 367806, 'TCTGG': 491183, 'TCCAA': 167188, 'TCCAT': 227889, 'TCCAC': 402754, 'TCCAG': 617331, 'TCCTA': 67494, 'TCCTT': 405188, 'TCCTC': 693436, 'TCCTG': 421661, 'TCCCA': 301123, 'TCCCT': 213915, 'TCCCC': 318765, 'TCCCG': 261375, 'TCCGA': 101289, 'TCCGT': 141179, 'TCCGC': 196918, 'TCCGG': 237976, 'TCGAA': 119241, 'TCGAT': 112128, 'TCGAC': 47640, 'TCGAG': 72290, 'TCGTA': 94638, 'TCGTT': 85595, 'TCGTC': 178930, 'TCGTG': 119377, 'TCGCA': 96410, 'TCGCT': 123879, 'TCGCC': 147610, 'TCGCG': 83774, 'TCGGA': 104065, 'TCGGT': 104938, 'TCGGC': 214706, 'TCGGG': 228766, 'TGAAA': 139771, 'TGAAT': 113142, 'TGAAC': 164047, 'TGAAG': 348260, 'TGATA': 70823, 'TGATT': 80260, 'TGATC': 182326, 'TGATG': 354737, 'TGACA': 170819, 'TGACT': 128805, 'TGACC': 205078, 'TGACG': 155627, 'TGAGA': 206515, 'TGAGT': 147549, 'TGAGC': 308418, 'TGAGG': 414443, 'TGTAA': 93540, 'TGTAT': 79788, 'TGTAC': 124508, 'TGTAG': 230369, 'TGTTA': 78037, 'TGTTT': 191613, 'TGTTC': 221170, 'TGTTG': 309319, 'TGTCA': 214882, 'TGTCT': 247050, 'TGTCC': 364831, 'TGTCG': 146370, 'TGTGA': 157935, 'TGTGT': 200895, 'TGTGC': 209590, 'TGTGG': 336555, 'TGCAA': 127185, 'TGCAT': 161503, 'TGCAC': 218679, 'TGCAG': 425117, 'TGCTA': 58475, 'TGCTT': 253865, 'TGCTC': 330583, 'TGCTG': 524867, 'TGCCA': 287173, 'TGCCT': 266690, 'TGCCC': 351854, 'TGCCG': 254150, 'TGCGA': 61217, 'TGCGT': 102030, 'TGCGC': 177887, 'TGCGG': 283998, 'TGGAA': 273366, 'TGGAT': 197814, 'TGGAC': 226781, 'TGGAG': 459209, 'TGGTA': 143333, 'TGGTT': 205225, 'TGGTC': 356929, 'TGGTG': 441948, 'TGGCA': 349977, 'TGGCT': 379677, 'TGGCC': 539298, 'TGGCG': 287241, 'TGGGA': 333090, 'TGGGT': 328685, 'TGGGC': 494303, 'TGGGG': 698927, 'CAAAA': 208304, 'CAAAT': 124540, 'CAAAC': 188174, 'CAAAG': 242742, 'CAATA': 68210, 'CAATT': 85053, 'CAATC': 110816, 'CAATG': 181278, 'CAACA': 142959, 'CAACT': 104136, 'CAACC': 104848, 'CAACG': 54252, 'CAAGA': 130723, 'CAAGT': 134367, 'CAAGC': 128466, 'CAAGG': 226875, 'CATAA': 78543, 'CATAT': 61139, 'CATAC': 105861, 'CATAG': 156687, 'CATTA': 71731, 'CATTT': 143889, 'CATTC': 170183, 'CATTG': 145500, 'CATCA': 261083, 'CATCT': 329148, 'CATCC': 281764, 'CATCG': 102715, 'CATGA': 235154, 'CATGT': 207330, 'CATGC': 198669, 'CATGG': 312098, 'CACAA': 201520, 'CACAT': 210640, 'CACAC': 255551, 'CACAG': 394984, 'CACTA': 57312, 'CACTT': 182507, 'CACTC': 209235, 'CACTG': 356319, 'CACCA': 424371, 'CACCT': 363992, 'CACCC': 270574, 'CACCG': 146507, 'CACGA': 137110, 'CACGT': 185017, 'CACGC': 191344, 'CACGG': 234617, 'CAGAA': 264503, 'CAGAT': 190172, 'CAGAC': 205644, 'CAGAG': 363497, 'CAGTA': 108523, 'CAGTT': 181941, 'CAGTC': 204696, 'CAGTG': 296577, 'CAGCA': 533871, 'CAGCT': 585690, 'CAGCC': 528474, 'CAGCG': 317105, 'CAGGA': 529478, 'CAGGT': 437533, 'CAGGC': 582184, 'CAGGG': 704817, 'CTAAA': 58840, 'CTAAT': 38132, 'CTAAC': 44290, 'CTAAG': 58246, 'CTATA': 26815, 'CTATT': 60603, 'CTATC': 52086, 'CTATG': 52834, 'CTACA': 68902, 'CTACT': 58692, 'CTACC': 57942, 'CTACG': 31439, 'CTAGA': 69493, 'CTAGT': 29059, 'CTAGC': 56403, 'CTAGG': 92950, 'CTTAA': 82522, 'CTTAT': 53781, 'CTTAC': 40461, 'CTTAG': 75856, 'CTTTA': 127674, 'CTTTT': 220767, 'CTTTC': 220100, 'CTTTG': 251088, 'CTTCA': 446581, 'CTTCT': 634922, 'CTTCC': 433770, 'CTTCG': 87519, 'CTTGA': 370987, 'CTTGT': 340748, 'CTTGC': 343604, 'CTTGG': 528981, 'CTCAA': 168933, 'CTCAT': 256533, 'CTCAC': 195488, 'CTCAG': 375296, 'CTCTA': 83669, 'CTCTT': 336459, 'CTCTC': 288568, 'CTCTG': 419922, 'CTCCA': 610152, 'CTCCT': 693011, 'CTCCC': 373755, 'CTCCG': 275612, 'CTCGA': 156304, 'CTCGT': 234106, 'CTCGC': 230203, 'CTCGG': 326213, 'CTGAA': 190431, 'CTGAT': 125721, 'CTGAC': 182403, 'CTGAG': 336429, 'CTGTA': 137619, 'CTGTT': 195503, 'CTGTC': 300523, 'CTGTG': 340637, 'CTGCA': 432332, 'CTGCT': 518402, 'CTGCC': 474407, 'CTGCG': 206513, 'CTGGA': 424583, 'CTGGT': 397951, 'CTGGC': 497108, 'CTGGG': 769850, 'CCAAA': 213760, 'CCAAT': 83864, 'CCAAC': 133517, 'CCAAG': 205068, 'CCATA': 105593, 'CCATT': 150511, 'CCATC': 256241, 'CCATG': 331775, 'CCACA': 360038, 'CCACT': 251898, 'CCACC': 489717, 'CCACG': 314484, 'CCAGA': 327380, 'CCAGT': 247336, 'CCAGC': 669053, 'CCAGG': 840581, 'CCTAA': 46242, 'CCTAT': 52484, 'CCTAC': 44390, 'CCTAG': 66078, 'CCTTA': 58826, 'CCTTT': 184018, 'CCTTC': 415906, 'CCTTG': 466162, 'CCTCA': 348268, 'CCTCT': 375862, 'CCTCC': 696764, 'CCTCG': 340681, 'CCTGA': 221662, 'CCTGT': 231570, 'CCTGC': 433213, 'CCTGG': 612983, 'CCCAA': 183641, 'CCCAT': 221804, 'CCCAC': 348164, 'CCCAG': 592209, 'CCCTA': 55995, 'CCCTT': 242398, 'CCCTC': 345022, 'CCCTG': 377515, 'CCCCA': 406623, 'CCCCT': 272842, 'CCCCC': 350608, 'CCCCG': 302740, 'CCCGA': 153634, 'CCCGT': 163015, 'CCCGC': 277838, 'CCCGG': 394631, 'CCGAA': 95875, 'CCGAT': 86009, 'CCGAC': 95418, 'CCGAG': 194821, 'CCGTA': 79021, 'CCGTT': 111635, 'CCGTC': 192863, 'CCGTG': 202282, 'CCGCA': 192769, 'CCGCT': 258692, 'CCGCC': 349019, 'CCGCG': 182399, 'CCGGA': 181372, 'CCGGT': 180977, 'CCGGC': 333742, 'CCGGG': 399377, 'CGAAA': 50147, 'CGAAT': 48522, 'CGAAC': 72714, 'CGAAG': 171580, 'CGATA': 23080, 'CGATT': 34901, 'CGATC': 96093, 'CGATG': 207828, 'CGACA': 67091, 'CGACT': 74019, 'CGACC': 86452, 'CGACG': 60518, 'CGAGA': 98445, 'CGAGT': 77673, 'CGAGC': 118307, 'CGAGG': 212573, 'CGTAA': 23853, 'CGTAT': 29253, 'CGTAC': 60067, 'CGTAG': 157725, 'CGTTA': 21141, 'CGTTT': 83718, 'CGTTC': 133857, 'CGTTG': 153956, 'CGTCA': 120330, 'CGTCT': 169322, 'CGTCC': 226565, 'CGTCG': 145940, 'CGTGA': 107459, 'CGTGT': 115675, 'CGTGC': 160941, 'CGTGG': 255357, 'CGCAA': 45724, 'CGCAT': 75522, 'CGCAC': 140019, 'CGCAG': 300823, 'CGCTA': 21831, 'CGCTT': 142069, 'CGCTC': 246045, 'CGCTG': 278803, 'CGCCA': 199042, 'CGCCT': 195524, 'CGCCC': 280542, 'CGCCG': 235039, 'CGCGA': 64146, 'CGCGT': 75258, 'CGCGC': 143389, 'CGCGG': 218568, 'CGGAA': 117265, 'CGGAT': 144076, 'CGGAC': 120431, 'CGGAG': 210524, 'CGGTA': 105286, 'CGGTT': 95187, 'CGGTC': 156577, 'CGGTG': 242730, 'CGGCA': 203652, 'CGGCT': 237453, 'CGGCC': 403395, 'CGGCG': 276305, 'CGGGA': 216017, 'CGGGT': 151262, 'CGGGC': 380021, 'CGGGG': 412558, 'GAAAA': 170251, 'GAAAT': 142506, 'GAAAC': 127354, 'GAAAG': 182352, 'GAATA': 69252, 'GAATT': 102702, 'GAATC': 113935, 'GAATG': 126069, 'GAACA': 173747, 'GAACT': 234550, 'GAACC': 132657, 'GAACG': 59201, 'GAAGA': 298383, 'GAAGT': 250210, 'GAAGC': 315546, 'GAAGG': 435972, 'GATAA': 62903, 'GATAT': 55575, 'GATAC': 59226, 'GATAG': 73771, 'GATTA': 40046, 'GATTT': 112321, 'GATTC': 92104, 'GATTG': 69244, 'GATCA': 158921, 'GATCT': 289711, 'GATCC': 149188, 'GATCG': 42742, 'GATGA': 313071, 'GATGT': 287804, 'GATGC': 238911, 'GATGG': 373837, 'GACAA': 116884, 'GACAT': 135416, 'GACAC': 167579, 'GACAG': 268089, 'GACTA': 36304, 'GACTT': 163221, 'GACTC': 178105, 'GACTG': 155617, 'GACCA': 229419, 'GACCT': 194744, 'GACCC': 204176, 'GACCG': 95844, 'GACGA': 103112, 'GACGT': 110236, 'GACGC': 120312, 'GACGG': 157534, 'GAGAA': 254880, 'GAGAT': 161504, 'GAGAC': 194561, 'GAGAG': 291055, 'GAGTA': 74604, 'GAGTT': 136490, 'GAGTC': 197567, 'GAGTG': 189260, 'GAGCA': 250356, 'GAGCT': 316167, 'GAGCC': 320435, 'GAGCG': 175186, 'GAGGA': 411602, 'GAGGT': 297023, 'GAGGC': 456892, 'GAGGG': 510043, 'GTAAA': 74859, 'GTAAT': 80083, 'GTAAC': 52266, 'GTAAG': 63759, 'GTATA': 41892, 'GTATT': 86369, 'GTATC': 60174, 'GTATG': 63590, 'GTACA': 134161, 'GTACT': 161541, 'GTACC': 80956, 'GTACG': 42474, 'GTAGA': 174921, 'GTAGT': 161760, 'GTAGC': 175425, 'GTAGG': 226176, 'GTTAA': 66414, 'GTTAT': 54083, 'GTTAC': 52496, 'GTTAG': 47245, 'GTTTA': 57466, 'GTTTT': 176977, 'GTTTC': 196141, 'GTTTG': 165391, 'GTTCA': 208105, 'GTTCT': 289848, 'GTTCC': 183224, 'GTTCG': 52083, 'GTTGA': 225441, 'GTTGT': 218030, 'GTTGC': 157306, 'GTTGG': 297745, 'GTCAA': 133686, 'GTCAT': 176856, 'GTCAC': 234134, 'GTCAG': 251261, 'GTCTA': 57280, 'GTCTT': 273221, 'GTCTC': 272976, 'GTCTG': 276874, 'GTCCA': 354459, 'GTCCT': 378231, 'GTCCC': 334389, 'GTCCG': 159939, 'GTCGA': 99295, 'GTCGT': 141834, 'GTCGC': 114278, 'GTCGG': 187327, 'GTGAA': 186684, 'GTGAT': 147780, 'GTGAC': 176186, 'GTGAG': 265881, 'GTGTA': 103493, 'GTGTT': 159545, 'GTGTC': 201644, 'GTGTG': 227947, 'GTGCA': 218800, 'GTGCT': 286673, 'GTGCC': 255375, 'GTGCG': 134447, 'GTGGA': 317733, 'GTGGT': 266026, 'GTGGC': 431838, 'GTGGG': 516445, 'GCAAA': 158295, 'GCAAT': 93601, 'GCAAC': 100103, 'GCAAG': 148261, 'GCATA': 90977, 'GCATT': 107697, 'GCATC': 210640, 'GCATG': 214193, 'GCACA': 256064, 'GCACT': 219415, 'GCACC': 267516, 'GCACG': 189559, 'GCAGA': 302978, 'GCAGT': 218271, 'GCAGC': 622401, 'GCAGG': 724261, 'GCTAA': 56655, 'GCTAT': 36481, 'GCTAC': 63704, 'GCTAG': 65701, 'GCTTA': 64546, 'GCTTT': 194161, 'GCTTC': 392135, 'GCTTG': 343069, 'GCTCA': 296348, 'GCTCT': 297185, 'GCTCC': 521124, 'GCTCG': 231040, 'GCTGA': 270928, 'GCTGT': 344393, 'GCTGC': 605823, 'GCTGG': 720566, 'GCCAA': 185946, 'GCCAT': 242128, 'GCCAC': 399034, 'GCCAG': 541497, 'GCCTA': 50977, 'GCCTT': 279395, 'GCCTC': 467925, 'GCCTG': 448582, 'GCCCA': 444772, 'GCCCT': 370073, 'GCCCC': 480819, 'GCCCG': 313453, 'GCCGA': 149885, 'GCCGT': 208339, 'GCCGC': 413940, 'GCCGG': 377423, 'GCGAA': 63900, 'GCGAT': 90226, 'GCGAC': 82046, 'GCGAG': 152095, 'GCGTA': 54689, 'GCGTT': 97641, 'GCGTC': 175154, 'GCGTG': 209639, 'GCGCA': 191479, 'GCGCT': 216751, 'GCGCC': 277656, 'GCGCG': 168314, 'GCGGA': 208614, 'GCGGT': 206153, 'GCGGC': 427224, 'GCGGG': 386163, 'GGAAA': 209012, 'GGAAT': 137529, 'GGAAC': 194946, 'GGAAG': 462713, 'GGATA': 62604, 'GGATT': 108380, 'GGATC': 224625, 'GGATG': 387632, 'GGACA': 281111, 'GGACT': 217623, 'GGACC': 249297, 'GGACG': 154569, 'GGAGA': 363841, 'GGAGT': 242932, 'GGAGC': 404509, 'GGAGG': 668864, 'GGTAA': 72264, 'GGTAT': 79968, 'GGTAC': 152478, 'GGTAG': 230136, 'GGTTA': 56120, 'GGTTT': 165574, 'GGTTC': 214364, 'GGTTG': 271718, 'GGTCA': 308793, 'GGTCT': 299781, 'GGTCC': 403012, 'GGTCG': 147877, 'GGTGA': 336684, 'GGTGT': 242627, 'GGTGC': 342951, 'GGTGG': 669016, 'GGCAA': 192800, 'GGCAT': 223679, 'GGCAC': 314015, 'GGCAG': 680485, 'GGCTA': 73167, 'GGCTT': 323869, 'GGCTC': 418216, 'GGCTG': 674417, 'GGCCA': 549826, 'GGCCT': 508657, 'GGCCC': 617252, 'GGCCG': 431103, 'GGCGA': 162939, 'GGCGT': 217522, 'GGCGC': 333933, 'GGCGG': 488629, 'GGGAA': 273860, 'GGGAT': 210381, 'GGGAC': 300884, 'GGGAG': 554114, 'GGGTA': 130322, 'GGGTT': 226726, 'GGGTC': 364292, 'GGGTG': 497161, 'GGGCA': 511481, 'GGGCT': 507724, 'GGGCC': 721233, 'GGGCG': 357816, 'GGGGA': 464609, 'GGGGT': 457520, 'GGGGC': 736465, 'GGGGG': 693772}, 'overrepresented_sequences': {}} {'total_reads': 3434219, 'total_bases': 198987583, 'q20_bases': 188809010, 'q30_bases': 179142422, 'total_cycles': 58, 'quality_curves': {'A': [36.0602, 36.0702, 36.0775, 36.1274, 36.1307, 36.1241, 36.143, 36.144, 36.1207, 36.1115, 36.0891, 36.1004, 36.0761, 36.0442, 36.0466, 35.9933, 36.0071, 35.984, 35.946, 35.9493, 35.8891, 35.8796, 35.8753, 35.8711, 35.8316, 35.775, 35.7363, 35.6907, 35.676, 35.6062, 35.5495, 35.5416, 35.495, 35.4743, 35.4524, 35.4023, 35.3619, 35.3113, 35.2796, 35.2726, 35.2819, 35.2857, 35.2008, 35.0961, 35.0479, 34.9571, 34.8645, 34.8091, 34.7189, 34.571, 34.3818, 34.1559, 34.0433, 33.8997, 33.896, 33.6844, 33.4699, 33.2933], 'T': [36.5543, 36.6206, 36.5845, 36.5808, 36.5862, 36.5684, 36.5389, 36.5281, 36.4959, 36.4698, 36.4469, 36.4405, 36.4053, 36.3844, 36.3595, 36.3318, 36.3126, 36.2937, 36.2352, 36.2203, 36.176, 36.1456, 36.1558, 36.0936, 36.0778, 36.0166, 35.9996, 35.9261, 35.8751, 35.8376, 35.7806, 35.7619, 35.7067, 35.6769, 35.6209, 35.6081, 35.532, 35.4956, 35.4359, 35.4082, 35.399, 35.4029, 35.3329, 35.2774, 35.1524, 35.0696, 35.0011, 34.9292, 34.828, 34.6805, 34.5255, 34.3121, 34.1651, 34.0832, 34.0073, 33.889, 33.6606, 33.5149], 'C': [36.5711, 36.5994, 36.5977, 36.5972, 36.5973, 36.6101, 36.6248, 36.6225, 36.6225, 36.5848, 36.5816, 36.5742, 36.5704, 36.5757, 36.5678, 36.5587, 36.5716, 36.5403, 36.5425, 36.5062, 36.5154, 36.5075, 36.4838, 36.4998, 36.4716, 36.4442, 36.4141, 36.3912, 36.3564, 36.3325, 36.2918, 36.2708, 36.2559, 36.2356, 36.2234, 36.2078, 36.1724, 36.1316, 36.0769, 36.0896, 36.066, 36.0608, 35.9926, 35.9173, 35.8498, 35.7714, 35.6756, 35.6282, 35.5104, 35.3952, 35.2761, 35.1036, 34.9112, 34.8412, 34.7075, 34.5572, 34.3562, 34.1616], 'G': [35.962, 36.0099, 36.0134, 36.0158, 36.0367, 36.0452, 36.0274, 36.037, 35.9977, 35.9955, 35.9772, 35.9691, 35.9649, 35.9554, 35.9514, 35.9239, 35.9015, 35.8834, 35.8601, 35.8296, 35.7837, 35.7882, 35.779, 35.7748, 35.7403, 35.6903, 35.6698, 35.6292, 35.5986, 35.5324, 35.5138, 35.5105, 35.4697, 35.4663, 35.4175, 35.4182, 35.3619, 35.3024, 35.2546, 35.271, 35.2425, 35.2475, 35.1727, 35.0974, 35.0075, 34.9405, 34.8552, 34.7956, 34.6808, 34.5719, 34.382, 34.1737, 33.9854, 33.8879, 33.8287, 33.6181, 33.4112, 33.2581], 'mean': [36.2846, 36.3192, 36.3129, 36.3184, 36.3289, 36.3295, 36.3261, 36.3312, 36.3076, 36.2869, 36.2727, 36.2686, 36.2535, 36.2427, 36.2354, 36.2059, 36.2036, 36.1822, 36.1547, 36.1324, 36.0985, 36.0883, 36.0833, 36.0759, 36.0439, 35.9977, 35.9717, 35.9269, 35.8946, 35.8505, 35.8014, 35.7904, 35.7555, 35.7347, 35.7032, 35.689, 35.6389, 35.5886, 35.5431, 35.5432, 35.5266, 35.5311, 35.4532, 35.3754, 35.291, 35.2115, 35.1302, 35.0744, 34.9662, 34.8375, 34.681, 34.4684, 34.3097, 34.2192, 34.1408, 33.9664, 33.7595, 33.5861]}, 'content_curves': {'A': [0.213159, 0.217693, 0.215115, 0.219132, 0.226503, 0.215772, 0.21884, 0.220436, 0.21762, 0.220053, 0.224801, 0.220265, 0.219186, 0.222621, 0.214927, 0.218169, 0.222778, 0.2184, 0.219876, 0.221746, 0.218637, 0.219001, 0.221936, 0.216122, 0.220885, 0.220952, 0.215698, 0.220131, 0.222506, 0.218015, 0.222125, 0.223195, 0.217428, 0.219482, 0.223322, 0.216315, 0.217678, 0.22407, 0.219872, 0.221503, 0.226431, 0.217074, 0.220919, 0.221453, 0.219428, 0.219156, 0.2241, 0.218176, 0.220465, 0.222015, 0.215774, 0.217604, 0.220707, 0.215421, 0.220153, 0.22318, 0.216091, 0.220075], 'T': [0.206193, 0.197531, 0.197297, 0.190166, 0.193081, 0.191377, 0.189683, 0.198807, 0.195274, 0.193183, 0.199246, 0.194175, 0.193006, 0.196974, 0.19607, 0.193917, 0.195077, 0.198471, 0.194157, 0.197405, 0.192441, 0.189693, 0.1966, 0.19121, 0.193231, 0.196903, 0.194389, 0.193874, 0.197066, 0.192035, 0.190489, 0.196367, 0.194189, 0.193803, 0.196237, 0.195859, 0.191167, 0.194737, 0.192911, 0.19012, 0.192352, 0.192143, 0.192486, 0.198351, 0.191855, 0.192435, 0.195542, 0.192456, 0.191257, 0.196772, 0.194167, 0.190256, 0.196054, 0.194734, 0.192051, 0.195396, 0.193328, 0.192084], 'C': [0.297772, 0.297761, 0.296087, 0.293512, 0.293976, 0.295977, 0.295573, 0.295348, 0.297526, 0.295696, 0.292564, 0.295862, 0.296034, 0.29528, 0.29797, 0.297754, 0.296106, 0.298542, 0.297409, 0.296621, 0.295669, 0.296389, 0.296276, 0.302609, 0.298422, 0.29777, 0.300581, 0.297415, 0.295946, 0.304266, 0.298089, 0.29402, 0.300192, 0.297251, 0.295325, 0.300825, 0.301798, 0.298234, 0.301811, 0.300381, 0.297533, 0.302783, 0.29823, 0.298927, 0.300606, 0.300072, 0.297917, 0.300612, 0.299935, 0.296907, 0.30334, 0.300408, 0.298698, 0.305018, 0.300124, 0.299582, 0.304107, 0.299838], 'G': [0.282823, 0.287015, 0.2915, 0.297191, 0.28644, 0.296874, 0.295898, 0.285409, 0.289581, 0.291067, 0.283388, 0.289698, 0.291773, 0.285122, 0.29103, 0.290122, 0.286039, 0.284567, 0.288557, 0.284184, 0.293249, 0.294891, 0.285189, 0.290059, 0.287462, 0.284375, 0.289324, 0.28858, 0.284482, 0.285684, 0.289207, 0.286418, 0.28814, 0.28938, 0.285116, 0.286986, 0.289353, 0.282939, 0.2854, 0.287993, 0.283684, 0.287976, 0.288334, 0.281196, 0.28792, 0.288132, 0.282441, 0.288752, 0.288342, 0.284307, 0.28672, 0.291513, 0.284534, 0.284828, 0.287646, 0.281818, 0.286474, 0.288003], 'N': [5.2996e-05, 0, 0, 0, 0, 0, 6.11493e-06, 0, 0, 0, 8.73561e-07, 0, 0, 3.20306e-06, 3.20306e-06, 3.75631e-05, 0, 2.06743e-05, 2.91187e-07, 4.42604e-05, 4.07662e-06, 2.53333e-05, 0, 0, 0, 0, 8.44448e-06, 8.73569e-07, 0, 0, 9.02696e-05, 0, 5.03814e-05, 8.44584e-05, 0, 1.51457e-05, 3.78665e-06, 2.00994e-05, 5.24363e-06, 3.78727e-06, 0, 2.3601e-05, 3.14706e-05, 7.31479e-05, 0.000191489, 0.000205508, 0, 4.3741e-06, 0, 0, 0, 0.00021934, 7.01431e-06, 0, 2.54891e-05, 2.40409e-05, 0, 0], 'GC': [0.580595, 0.584776, 0.587587, 0.590703, 0.580416, 0.592851, 0.591472, 0.580757, 0.587106, 0.586763, 0.575952, 0.585561, 0.587808, 0.580402, 0.589, 0.587876, 0.582145, 0.583109, 0.585966, 0.580805, 0.588918, 0.59128, 0.581464, 0.592668, 0.585883, 0.582145, 0.589905, 0.585994, 0.580427, 0.58995, 0.587296, 0.580438, 0.588332, 0.586631, 0.580441, 0.587811, 0.591151, 0.581172, 0.587211, 0.588374, 0.581217, 0.590759, 0.586564, 0.580123, 0.588526, 0.588203, 0.580358, 0.589364, 0.588278, 0.581214, 0.59006, 0.591921, 0.583232, 0.589845, 0.58777, 0.5814, 0.590581, 0.58784]}, 'kmer_count': {'AAAAA': 316580, 'AAAAT': 133127, 'AAAAC': 161139, 'AAAAG': 187803, 'AAATA': 102496, 'AAATT': 104285, 'AAATC': 91497, 'AAATG': 123782, 'AAACA': 180884, 'AAACT': 124666, 'AAACC': 149150, 'AAACG': 71196, 'AAAGA': 252403, 'AAAGT': 135801, 'AAAGC': 175411, 'AAAGG': 164483, 'AATAA': 66638, 'AATAT': 64596, 'AATAC': 66657, 'AATAG': 43612, 'AATTA': 56580, 'AATTT': 105749, 'AATTC': 81337, 'AATTG': 64484, 'AATCA': 68452, 'AATCT': 80010, 'AATCC': 101899, 'AATCG': 27784, 'AATGA': 108041, 'AATGT': 114783, 'AATGC': 95799, 'AATGG': 136045, 'AACAA': 215453, 'AACAT': 174674, 'AACAC': 124523, 'AACAG': 179384, 'AACTA': 62734, 'AACTT': 132999, 'AACTC': 118473, 'AACTG': 159965, 'AACCA': 178534, 'AACCT': 162558, 'AACCC': 192265, 'AACCG': 81027, 'AACGA': 74467, 'AACGT': 79112, 'AACGC': 85396, 'AACGG': 98582, 'AAGAA': 470001, 'AAGAT': 265385, 'AAGAC': 240127, 'AAGAG': 302920, 'AAGTA': 110308, 'AAGTT': 159061, 'AAGTC': 156716, 'AAGTG': 160147, 'AAGCA': 222077, 'AAGCT': 252423, 'AAGCC': 282568, 'AAGCG': 140206, 'AAGGA': 352993, 'AAGGT': 194524, 'AAGGC': 262221, 'AAGGG': 218384, 'ATAAA': 83894, 'ATAAT': 39132, 'ATAAC': 45441, 'ATAAG': 44793, 'ATATA': 42758, 'ATATT': 62035, 'ATATC': 53487, 'ATATG': 51465, 'ATACA': 69405, 'ATACT': 51754, 'ATACC': 57204, 'ATACG': 25223, 'ATAGA': 46521, 'ATAGT': 35835, 'ATAGC': 35159, 'ATAGG': 39960, 'ATTAA': 64911, 'ATTAT': 48777, 'ATTAC': 67573, 'ATTAG': 27041, 'ATTTA': 68609, 'ATTTT': 152495, 'ATTTC': 118769, 'ATTTG': 97280, 'ATTCA': 102811, 'ATTCT': 101566, 'ATTCC': 102967, 'ATTCG': 47886, 'ATTGA': 125417, 'ATTGT': 89966, 'ATTGC': 81897, 'ATTGG': 72618, 'ATCAA': 188502, 'ATCAT': 162590, 'ATCAC': 125276, 'ATCAG': 114988, 'ATCTA': 71179, 'ATCTT': 149937, 'ATCTC': 146303, 'ATCTG': 165248, 'ATCCA': 180394, 'ATCCT': 204085, 'ATCCC': 177552, 'ATCCG': 132941, 'ATCGA': 106964, 'ATCGT': 66960, 'ATCGC': 74719, 'ATCGG': 70169, 'ATGAA': 202188, 'ATGAT': 165232, 'ATGAC': 150991, 'ATGAG': 228721, 'ATGTA': 68214, 'ATGTT': 101636, 'ATGTC': 138097, 'ATGTG': 186912, 'ATGCA': 136654, 'ATGCT': 142455, 'ATGCC': 206789, 'ATGCG': 67257, 'ATGGA': 217711, 'ATGGT': 151068, 'ATGGC': 240532, 'ATGGG': 195067, 'ACAAA': 182559, 'ACAAT': 72612, 'ACAAC': 181598, 'ACAAG': 329731, 'ACATA': 44091, 'ACATT': 103909, 'ACATC': 240676, 'ACATG': 189303, 'ACACA': 170241, 'ACACT': 104576, 'ACACC': 194302, 'ACACG': 98547, 'ACAGA': 214080, 'ACAGT': 135063, 'ACAGC': 295177, 'ACAGG': 206481, 'ACTAA': 46422, 'ACTAT': 62351, 'ACTAC': 136453, 'ACTAG': 24707, 'ACTTA': 45507, 'ACTTT': 131564, 'ACTTC': 212498, 'ACTTG': 100289, 'ACTCA': 120588, 'ACTCT': 117734, 'ACTCC': 203594, 'ACTCG': 67826, 'ACTGA': 153272, 'ACTGT': 135197, 'ACTGC': 201581, 'ACTGG': 225956, 'ACCAA': 233795, 'ACCAT': 213884, 'ACCAC': 214048, 'ACCAG': 354687, 'ACCTA': 93627, 'ACCTT': 150778, 'ACCTC': 250468, 'ACCTG': 387927, 'ACCCA': 267011, 'ACCCT': 241643, 'ACCCC': 371684, 'ACCCG': 141573, 'ACCGA': 90848, 'ACCGT': 85634, 'ACCGC': 200842, 'ACCGG': 153490, 'ACGAA': 44609, 'ACGAT': 56697, 'ACGAC': 116906, 'ACGAG': 204460, 'ACGTA': 24848, 'ACGTT': 33637, 'ACGTC': 89216, 'ACGTG': 161893, 'ACGCA': 102204, 'ACGCT': 147670, 'ACGCC': 199329, 'ACGCG': 72689, 'ACGGA': 139441, 'ACGGT': 64570, 'ACGGC': 188851, 'ACGGG': 141177, 'AGAAA': 293134, 'AGAAT': 124740, 'AGAAC': 241315, 'AGAAG': 567013, 'AGATA': 65958, 'AGATT': 118959, 'AGATC': 247138, 'AGATG': 296180, 'AGACA': 214984, 'AGACT': 146575, 'AGACC': 254770, 'AGACG': 165503, 'AGAGA': 281561, 'AGAGT': 149206, 'AGAGC': 283064, 'AGAGG': 327576, 'AGTAA': 44988, 'AGTAT': 80983, 'AGTAC': 148130, 'AGTAG': 57058, 'AGTTA': 57088, 'AGTTT': 132471, 'AGTTC': 209871, 'AGTTG': 93266, 'AGTCA': 124315, 'AGTCT': 109619, 'AGTCC': 198867, 'AGTCG': 72627, 'AGTGA': 184669, 'AGTGT': 116027, 'AGTGC': 186021, 'AGTGG': 249571, 'AGCAA': 184526, 'AGCAT': 125844, 'AGCAC': 237306, 'AGCAG': 471827, 'AGCTA': 85921, 'AGCTT': 138768, 'AGCTC': 279684, 'AGCTG': 531185, 'AGCCA': 355689, 'AGCCT': 328743, 'AGCCC': 445163, 'AGCCG': 241799, 'AGCGA': 119095, 'AGCGT': 96400, 'AGCGC': 204569, 'AGCGG': 276873, 'AGGAA': 271059, 'AGGAT': 179402, 'AGGAC': 339248, 'AGGAG': 629609, 'AGGTA': 69798, 'AGGTT': 109082, 'AGGTC': 186279, 'AGGTG': 339768, 'AGGCA': 232717, 'AGGCT': 247063, 'AGGCC': 463167, 'AGGCG': 199243, 'AGGGA': 196532, 'AGGGT': 155466, 'AGGGC': 326078, 'AGGGG': 238188, 'TAAAA': 102494, 'TAAAT': 54521, 'TAAAC': 52815, 'TAAAG': 102625, 'TAATA': 25158, 'TAATT': 53729, 'TAATC': 35369, 'TAATG': 60753, 'TAACA': 62641, 'TAACT': 56515, 'TAACC': 48528, 'TAACG': 16924, 'TAAGA': 73791, 'TAAGT': 37535, 'TAAGC': 49229, 'TAAGG': 53306, 'TATAA': 40539, 'TATAT': 52077, 'TATAC': 32205, 'TATAG': 26316, 'TATTA': 42622, 'TATTT': 121928, 'TATTC': 54837, 'TATTG': 58669, 'TATCA': 60524, 'TATCT': 80294, 'TATCC': 63181, 'TATCG': 20562, 'TATGA': 100452, 'TATGT': 79756, 'TATGC': 77650, 'TATGG': 91007, 'TACAA': 159879, 'TACAT': 92363, 'TACAC': 87324, 'TACAG': 121424, 'TACTA': 53943, 'TACTT': 79596, 'TACTC': 61629, 'TACTG': 106298, 'TACCA': 128624, 'TACCT': 134136, 'TACCC': 113118, 'TACCG': 92317, 'TACGA': 95086, 'TACGT': 36730, 'TACGC': 61387, 'TACGG': 69865, 'TAGAA': 57537, 'TAGAT': 34541, 'TAGAC': 44168, 'TAGAG': 79097, 'TAGTA': 25977, 'TAGTT': 32236, 'TAGTC': 35959, 'TAGTG': 56894, 'TAGCA': 49326, 'TAGCT': 58291, 'TAGCC': 65090, 'TAGCG': 22417, 'TAGGA': 61449, 'TAGGT': 29875, 'TAGGC': 43065, 'TAGGG': 46271, 'TTAAA': 96547, 'TTAAT': 53323, 'TTAAC': 54067, 'TTAAG': 63933, 'TTATA': 42866, 'TTATT': 90910, 'TTATC': 49037, 'TTATG': 70886, 'TTACA': 74539, 'TTACT': 68303, 'TTACC': 60896, 'TTACG': 18669, 'TTAGA': 43323, 'TTAGT': 36794, 'TTAGC': 50492, 'TTAGG': 41645, 'TTTAA': 99022, 'TTTAT': 110595, 'TTTAC': 56286, 'TTTAG': 51336, 'TTTTA': 133714, 'TTTTT': 293487, 'TTTTC': 140329, 'TTTTG': 167014, 'TTTCA': 132703, 'TTTCT': 183631, 'TTTCC': 171904, 'TTTCG': 52709, 'TTTGA': 163790, 'TTTGT': 160541, 'TTTGC': 140054, 'TTTGG': 183851, 'TTCAA': 186535, 'TTCAT': 139996, 'TTCAC': 162399, 'TTCAG': 186331, 'TTCTA': 98088, 'TTCTT': 172025, 'TTCTC': 223530, 'TTCTG': 221405, 'TTCCA': 214645, 'TTCCT': 291099, 'TTCCC': 229228, 'TTCCG': 114243, 'TTCGA': 105781, 'TTCGT': 70136, 'TTCGC': 64739, 'TTCGG': 99184, 'TTGAA': 127353, 'TTGAT': 84492, 'TTGAC': 120196, 'TTGAG': 147366, 'TTGTA': 67876, 'TTGTT': 102930, 'TTGTC': 109224, 'TTGTG': 167454, 'TTGCA': 109770, 'TTGCT': 123763, 'TTGCC': 161105, 'TTGCG': 49345, 'TTGGA': 144215, 'TTGGT': 89657, 'TTGGC': 154993, 'TTGGG': 165044, 'TCAAA': 148403, 'TCAAT': 69539, 'TCAAC': 198352, 'TCAAG': 309233, 'TCATA': 46226, 'TCATT': 100029, 'TCATC': 253972, 'TCATG': 205294, 'TCACA': 136577, 'TCACT': 148714, 'TCACC': 286637, 'TCACG': 91977, 'TCAGA': 186577, 'TCAGT': 129581, 'TCAGC': 245813, 'TCAGG': 201088, 'TCTAA': 39443, 'TCTAT': 66314, 'TCTAC': 164956, 'TCTAG': 58943, 'TCTTA': 56987, 'TCTTT': 175859, 'TCTTC': 276606, 'TCTTG': 124457, 'TCTCA': 172948, 'TCTCT': 203930, 'TCTCC': 312955, 'TCTCG': 96391, 'TCTGA': 161816, 'TCTGT': 191196, 'TCTGC': 264049, 'TCTGG': 305464, 'TCCAA': 205009, 'TCCAT': 143672, 'TCCAC': 260642, 'TCCAG': 370863, 'TCCTA': 102678, 'TCCTT': 175747, 'TCCTC': 340763, 'TCCTG': 477250, 'TCCCA': 286048, 'TCCCT': 262231, 'TCCCC': 378794, 'TCCCG': 192941, 'TCCGA': 106090, 'TCCGT': 86657, 'TCCGC': 201824, 'TCCGG': 191374, 'TCGAA': 51395, 'TCGAT': 38595, 'TCGAC': 98660, 'TCGAG': 151794, 'TCGTA': 25762, 'TCGTT': 27167, 'TCGTC': 89558, 'TCGTG': 138109, 'TCGCA': 57923, 'TCGCT': 87838, 'TCGCC': 142522, 'TCGCG': 67563, 'TCGGA': 109988, 'TCGGT': 66291, 'TCGGC': 164070, 'TCGGG': 150061, 'TGAAA': 157569, 'TGAAT': 96959, 'TGAAC': 174667, 'TGAAG': 395370, 'TGATA': 54746, 'TGATT': 85853, 'TGATC': 149575, 'TGATG': 237617, 'TGACA': 176742, 'TGACT': 126947, 'TGACC': 283730, 'TGACG': 108995, 'TGAGA': 185365, 'TGAGT': 128401, 'TGAGC': 270564, 'TGAGG': 321944, 'TGTAA': 79752, 'TGTAT': 73087, 'TGTAC': 103976, 'TGTAG': 61173, 'TGTTA': 60480, 'TGTTT': 157336, 'TGTTC': 153343, 'TGTTG': 124411, 'TGTCA': 150136, 'TGTCT': 164682, 'TGTCC': 246670, 'TGTCG': 76056, 'TGTGA': 203770, 'TGTGT': 185599, 'TGTGC': 235429, 'TGTGG': 327053, 'TGCAA': 140228, 'TGCAT': 104054, 'TGCAC': 181024, 'TGCAG': 417614, 'TGCTA': 74223, 'TGCTT': 142548, 'TGCTC': 216039, 'TGCTG': 524184, 'TGCCA': 291861, 'TGCCT': 312361, 'TGCCC': 423486, 'TGCCG': 194223, 'TGCGA': 82331, 'TGCGT': 79978, 'TGCGC': 182928, 'TGCGG': 195528, 'TGGAA': 216985, 'TGGAT': 176361, 'TGGAC': 345671, 'TGGAG': 564643, 'TGGTA': 77293, 'TGGTT': 111291, 'TGGTC': 208022, 'TGGTG': 393639, 'TGGCA': 252896, 'TGGCT': 301529, 'TGGCC': 514620, 'TGGCG': 214181, 'TGGGA': 278821, 'TGGGT': 182682, 'TGGGC': 412700, 'TGGGG': 365186, 'CAAAA': 184408, 'CAAAT': 114921, 'CAAAC': 142106, 'CAAAG': 233789, 'CAATA': 51244, 'CAATT': 59108, 'CAATC': 67563, 'CAATG': 132483, 'CAACA': 263562, 'CAACT': 142201, 'CAACC': 226774, 'CAACG': 135832, 'CAAGA': 446270, 'CAAGT': 221031, 'CAAGC': 302876, 'CAAGG': 434305, 'CATAA': 47869, 'CATAT': 41652, 'CATAC': 54796, 'CATAG': 46387, 'CATTA': 58590, 'CATTT': 109341, 'CATTC': 110964, 'CATTG': 152969, 'CATCA': 304005, 'CATCT': 247435, 'CATCC': 318768, 'CATCG': 184326, 'CATGA': 216482, 'CATGT': 159518, 'CATGC': 194252, 'CATGG': 316975, 'CACAA': 160850, 'CACAT': 121219, 'CACAC': 186930, 'CACAG': 294230, 'CACTA': 79629, 'CACTT': 135371, 'CACTC': 145328, 'CACTG': 260595, 'CACCA': 382298, 'CACCT': 324968, 'CACCC': 387677, 'CACCG': 205945, 'CACGA': 99821, 'CACGT': 92488, 'CACGC': 199016, 'CACGG': 180973, 'CAGAA': 296451, 'CAGAT': 209159, 'CAGAC': 248148, 'CAGAG': 370380, 'CAGTA': 98220, 'CAGTT': 154445, 'CAGTC': 149807, 'CAGTG': 311342, 'CAGCA': 448473, 'CAGCT': 396358, 'CAGCC': 619173, 'CAGCG': 286497, 'CAGGA': 372541, 'CAGGT': 239320, 'CAGGC': 398745, 'CAGGG': 327976, 'CTAAA': 70164, 'CTAAT': 38210, 'CTAAC': 36633, 'CTAAG': 66908, 'CTATA': 39324, 'CTATT': 65680, 'CTATC': 72983, 'CTATG': 137556, 'CTACA': 201352, 'CTACT': 105428, 'CTACC': 223494, 'CTACG': 163990, 'CTAGA': 60523, 'CTAGT': 38110, 'CTAGC': 53123, 'CTAGG': 55788, 'CTTAA': 58768, 'CTTAT': 56659, 'CTTAC': 49895, 'CTTAG': 55301, 'CTTTA': 63353, 'CTTTT': 169055, 'CTTTC': 173976, 'CTTTG': 221813, 'CTTCA': 293058, 'CTTCT': 273764, 'CTTCC': 408051, 'CTTCG': 157022, 'CTTGA': 118284, 'CTTGT': 108470, 'CTTGC': 131478, 'CTTGG': 172556, 'CTCAA': 215170, 'CTCAT': 174495, 'CTCAC': 220749, 'CTCAG': 290565, 'CTCTA': 92671, 'CTCTT': 182981, 'CTCTC': 240592, 'CTCTG': 330661, 'CTCCA': 377195, 'CTCCT': 373982, 'CTCCC': 439670, 'CTCCG': 219451, 'CTCGA': 72193, 'CTCGT': 68117, 'CTCGC': 127336, 'CTCGG': 209395, 'CTGAA': 271238, 'CTGAT': 162009, 'CTGAC': 224473, 'CTGAG': 337396, 'CTGTA': 114576, 'CTGTT': 156508, 'CTGTC': 238085, 'CTGTG': 357876, 'CTGCA': 389853, 'CTGCT': 458390, 'CTGCC': 578305, 'CTGCG': 274059, 'CTGGA': 564189, 'CTGGT': 304537, 'CTGGC': 506846, 'CTGGG': 552544, 'CCAAA': 198646, 'CCAAT': 91899, 'CCAAC': 249635, 'CCAAG': 452810, 'CCATA': 67241, 'CCATT': 130165, 'CCATC': 350743, 'CCATG': 319859, 'CCACA': 282975, 'CCACT': 216676, 'CCACC': 520810, 'CCACG': 230731, 'CCAGA': 406246, 'CCAGT': 241696, 'CCAGC': 635440, 'CCAGG': 539630, 'CCTAA': 67625, 'CCTAT': 100358, 'CCTAC': 218599, 'CCTAG': 73300, 'CCTTA': 71371, 'CCTTT': 174622, 'CCTTC': 372951, 'CCTTG': 184226, 'CCTCA': 356087, 'CCTCT': 306915, 'CCTCC': 545724, 'CCTCG': 201491, 'CCTGA': 339744, 'CCTGT': 260048, 'CCTGC': 629493, 'CCTGG': 773213, 'CCCAA': 268242, 'CCCAT': 203900, 'CCCAC': 394380, 'CCCAG': 648978, 'CCCTA': 133709, 'CCCTT': 229841, 'CCCTC': 396819, 'CCCTG': 604850, 'CCCCA': 535173, 'CCCCT': 428529, 'CCCCC': 674146, 'CCCCG': 373371, 'CCCGA': 209502, 'CCCGT': 135880, 'CCCGC': 338902, 'CCCGG': 384662, 'CCGAA': 84842, 'CCGAT': 58938, 'CCGAC': 176934, 'CCGAG': 303528, 'CCGTA': 38521, 'CCGTT': 53365, 'CCGTC': 154335, 'CCGTG': 209066, 'CCGCA': 234081, 'CCGCT': 228883, 'CCGCC': 490182, 'CCGCG': 231174, 'CCGGA': 232976, 'CCGGT': 85325, 'CCGGC': 351265, 'CCGGG': 385266, 'CGAAA': 44372, 'CGAAT': 43778, 'CGAAC': 53660, 'CGAAG': 95355, 'CGATA': 23300, 'CGATT': 41009, 'CGATC': 42229, 'CGATG': 105422, 'CGACA': 131605, 'CGACT': 94608, 'CGACC': 161026, 'CGACG': 123849, 'CGAGA': 207482, 'CGAGT': 124482, 'CGAGC': 214949, 'CGAGG': 325238, 'CGTAA': 25168, 'CGTAT': 18736, 'CGTAC': 40698, 'CGTAG': 27318, 'CGTTA': 12816, 'CGTTT': 43662, 'CGTTC': 52148, 'CGTTG': 51221, 'CGTCA': 128149, 'CGTCT': 124333, 'CGTCC': 152651, 'CGTCG': 61475, 'CGTGA': 112863, 'CGTGT': 103214, 'CGTGC': 194945, 'CGTGG': 285942, 'CGCAA': 142368, 'CGCAT': 109791, 'CGCAC': 125201, 'CGCAG': 189304, 'CGCTA': 80428, 'CGCTT': 107311, 'CGCTC': 158593, 'CGCTG': 312017, 'CGCCA': 281256, 'CGCCT': 267012, 'CGCCC': 322850, 'CGCCG': 274305, 'CGCGA': 93358, 'CGCGT': 72574, 'CGCGC': 176669, 'CGCGG': 205160, 'CGGAA': 164124, 'CGGAT': 82693, 'CGGAC': 159916, 'CGGAG': 306239, 'CGGTA': 32496, 'CGGTT': 50836, 'CGGTC': 85329, 'CGGTG': 142445, 'CGGCA': 235061, 'CGGCT': 225481, 'CGGCC': 425313, 'CGGCG': 293475, 'CGGGA': 249171, 'CGGGT': 113735, 'CGGGC': 304260, 'CGGGG': 299926, 'GAAAA': 197809, 'GAAAT': 120279, 'GAAAC': 170595, 'GAAAG': 203737, 'GAATA': 62801, 'GAATT': 90946, 'GAATC': 84457, 'GAATG': 138145, 'GAACA': 188082, 'GAACT': 151003, 'GAACC': 189676, 'GAACG': 113628, 'GAAGA': 509351, 'GAAGT': 190943, 'GAAGC': 370829, 'GAAGG': 375531, 'GATAA': 58380, 'GATAT': 52258, 'GATAC': 49485, 'GATAG': 40967, 'GATTA': 50648, 'GATTT': 101020, 'GATTC': 107835, 'GATTG': 94364, 'GATCA': 158592, 'GATCT': 124458, 'GATCC': 211353, 'GATCG': 85794, 'GATGA': 321792, 'GATGT': 141900, 'GATGC': 185369, 'GATGG': 259524, 'GACAA': 232382, 'GACAT': 189389, 'GACAC': 169641, 'GACAG': 256141, 'GACTA': 73764, 'GACTT': 142321, 'GACTC': 184362, 'GACTG': 186984, 'GACCA': 329568, 'GACCT': 261547, 'GACCC': 332879, 'GACCG': 152577, 'GACGA': 153873, 'GACGT': 102137, 'GACGC': 176330, 'GACGG': 184906, 'GAGAA': 405055, 'GAGAT': 219825, 'GAGAC': 248994, 'GAGAG': 287673, 'GAGTA': 96915, 'GAGTT': 146284, 'GAGTC': 162742, 'GAGTG': 207713, 'GAGCA': 298528, 'GAGCT': 330163, 'GAGCC': 406164, 'GAGCG': 248946, 'GAGGA': 632633, 'GAGGT': 240448, 'GAGGC': 437849, 'GAGGG': 322406, 'GTAAA': 62355, 'GTAAT': 44550, 'GTAAC': 48388, 'GTAAG': 38630, 'GTATA': 26167, 'GTATT': 60920, 'GTATC': 48822, 'GTATG': 88430, 'GTACA': 114985, 'GTACT': 75669, 'GTACC': 127251, 'GTACG': 54654, 'GTAGA': 64626, 'GTAGT': 39511, 'GTAGC': 56512, 'GTAGG': 42981, 'GTTAA': 44785, 'GTTAT': 37669, 'GTTAC': 48230, 'GTTAG': 37297, 'GTTTA': 50800, 'GTTTT': 118317, 'GTTTC': 108318, 'GTTTG': 163192, 'GTTCA': 145447, 'GTTCT': 155236, 'GTTCC': 164870, 'GTTCG': 81241, 'GTTGA': 72468, 'GTTGT': 88042, 'GTTGC': 89039, 'GTTGG': 123445, 'GTCAA': 132847, 'GTCAT': 126492, 'GTCAC': 154374, 'GTCAG': 170772, 'GTCTA': 67111, 'GTCTT': 127796, 'GTCTC': 174030, 'GTCTG': 202598, 'GTCCA': 206938, 'GTCCT': 227495, 'GTCCC': 272042, 'GTCCG': 116213, 'GTCGA': 54407, 'GTCGT': 75386, 'GTCGC': 88729, 'GTCGG': 107507, 'GTGAA': 223474, 'GTGAT': 116102, 'GTGAC': 199872, 'GTGAG': 189012, 'GTGTA': 67248, 'GTGTT': 133127, 'GTGTC': 150346, 'GTGTG': 237860, 'GTGCA': 205284, 'GTGCT': 232366, 'GTGCC': 278283, 'GTGCG': 149537, 'GTGGA': 375134, 'GTGGT': 245242, 'GTGGC': 378197, 'GTGGG': 325689, 'GCAAA': 146319, 'GCAAT': 76224, 'GCAAC': 140983, 'GCAAG': 312308, 'GCATA': 32813, 'GCATT': 96547, 'GCATC': 211114, 'GCATG': 171736, 'GCACA': 174454, 'GCACT': 151923, 'GCACC': 301325, 'GCACG': 151303, 'GCAGA': 317721, 'GCAGT': 208120, 'GCAGC': 574211, 'GCAGG': 392793, 'GCTAA': 58373, 'GCTAT': 86882, 'GCTAC': 173558, 'GCTAG': 50253, 'GCTTA': 46706, 'GCTTT': 146308, 'GCTTC': 270472, 'GCTTG': 120567, 'GCTCA': 250667, 'GCTCT': 218703, 'GCTCC': 350985, 'GCTCG': 110471, 'GCTGA': 340463, 'GCTGT': 278374, 'GCTGC': 608137, 'GCTGG': 622361, 'GCCAA': 287027, 'GCCAT': 305501, 'GCCAC': 382285, 'GCCAG': 449024, 'GCCTA': 129575, 'GCCTT': 246393, 'GCCTC': 421311, 'GCCTG': 530537, 'GCCCA': 429282, 'GCCCT': 432832, 'GCCCC': 596364, 'GCCCG': 360915, 'GCCGA': 217922, 'GCCGT': 147341, 'GCCGC': 441144, 'GCCGG': 323671, 'GCGAA': 56041, 'GCGAT': 57663, 'GCGAC': 118366, 'GCGAG': 212253, 'GCGTA': 22633, 'GCGTT': 45235, 'GCGTC': 133304, 'GCGTG': 189223, 'GCGCA': 172006, 'GCGCT': 194357, 'GCGCC': 314688, 'GCGCG': 174581, 'GCGGA': 226288, 'GCGGT': 94241, 'GCGGC': 473233, 'GCGGG': 290881, 'GGAAA': 196689, 'GGAAT': 111267, 'GGAAC': 172971, 'GGAAG': 392717, 'GGATA': 57490, 'GGATT': 108432, 'GGATC': 136857, 'GGATG': 269390, 'GGACA': 324336, 'GGACT': 219643, 'GGACC': 379539, 'GGACG': 219310, 'GGAGA': 486945, 'GGAGT': 213233, 'GGAGC': 512410, 'GGAGG': 658126, 'GGTAA': 44533, 'GGTAT': 52075, 'GGTAC': 80165, 'GGTAG': 58200, 'GGTTA': 37925, 'GGTTT': 108352, 'GGTTC': 131023, 'GGTTG': 102827, 'GGTCA': 180211, 'GGTCT': 172188, 'GGTCC': 225352, 'GGTCG': 115358, 'GGTGA': 225836, 'GGTGT': 184699, 'GGTGC': 250313, 'GGTGG': 463270, 'GGCAA': 209364, 'GGCAT': 172538, 'GGCAC': 234352, 'GGCAG': 414959, 'GGCTA': 128395, 'GGCTT': 195949, 'GGCTC': 278673, 'GGCTG': 485833, 'GGCCA': 496267, 'GGCCT': 423245, 'GGCCC': 631466, 'GGCCG': 420045, 'GGCGA': 148438, 'GGCGT': 142134, 'GGCGC': 291247, 'GGCGG': 403341, 'GGGAA': 221732, 'GGGAT': 133975, 'GGGAC': 298673, 'GGGAG': 367607, 'GGGTA': 55923, 'GGGTT': 108664, 'GGGTC': 212243, 'GGGTG': 246993, 'GGGCA': 312442, 'GGGCT': 315056, 'GGGCC': 568538, 'GGGCG': 273920, 'GGGGA': 298883, 'GGGGT': 172410, 'GGGGC': 430065, 'GGGGG': 300861}, 'overrepresented_sequences': {}} {'passed_filter_reads': 6868438, 'low_quality_reads': 153166, 'too_many_N_reads': 2046, 'too_short_reads': 82, 'too_long_reads': 0} fastp -i /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045019_1.fastq.gz -I /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045019_2.fastq.gz -o /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/.trimmed_1.fastq.gz -O /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/.trimmed_2.fastq.gz -l 25 --adapter_fasta /home/ubuntu/workspace/rnaseq/team_exercise/references/illumina_multiplex.fa --trim_tail1 5 --trim_tail2 5 --json /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/.fastp.json --html /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/.fastp.html SRR10045020_1 {'adapter_trimmed_reads': 48690, 'adapter_trimmed_bases': 306240, 'read1_adapter_sequence': 'unspecified', 'read2_adapter_sequence': 'unspecified', 'read1_adapter_counts': {'A': 734, 'C': 867, 'G': 941, 'T': 780, 'GG': 260, 'TG': 247, 'others': 20514}, 'read2_adapter_counts': {'A': 934, 'C': 862, 'G': 866, 'T': 660, 'AG': 248, 'CA': 254, 'CC': 258, 'others': 20263}} {'rate': 0.136352} {'peak': 85, 'unknown': 1079465, 'histogram': [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 64, 64, 59, 60, 63, 73, 71, 63, 74, 89, 100, 122, 129, 158, 180, 207, 246, 284, 335, 418, 475, 534, 614, 712, 803, 1006, 1092, 1233, 1374, 1594, 1782, 1887, 2005, 2321, 2522, 2708, 2973, 3204, 3437, 3581, 3855, 4199, 4445, 4737, 4995, 5335, 5379, 5612, 5792, 6281, 6351, 6622, 6824, 6905, 7294, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 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'ACTGA': 170510, 'ACTGT': 155478, 'ACTGC': 229916, 'ACTGG': 260670, 'ACCAA': 264465, 'ACCAT': 245496, 'ACCAC': 249459, 'ACCAG': 399827, 'ACCTA': 105514, 'ACCTT': 175638, 'ACCTC': 286993, 'ACCTG': 440953, 'ACCCA': 305082, 'ACCCT': 274380, 'ACCCC': 439720, 'ACCCG': 160791, 'ACCGA': 104503, 'ACCGT': 100214, 'ACCGC': 229852, 'ACCGG': 175515, 'ACGAA': 51697, 'ACGAT': 62496, 'ACGAC': 134341, 'ACGAG': 231580, 'ACGTA': 29146, 'ACGTT': 38309, 'ACGTC': 102974, 'ACGTG': 183519, 'ACGCA': 117044, 'ACGCT': 164560, 'ACGCC': 227421, 'ACGCG': 82299, 'ACGGA': 157955, 'ACGGT': 74864, 'ACGGC': 217670, 'ACGGG': 162947, 'AGAAA': 331238, 'AGAAT': 144336, 'AGAAC': 273140, 'AGAAG': 633306, 'AGATA': 74829, 'AGATT': 138493, 'AGATC': 286527, 'AGATG': 331518, 'AGACA': 246164, 'AGACT': 165228, 'AGACC': 292040, 'AGACG': 186054, 'AGAGA': 319660, 'AGAGT': 167853, 'AGAGC': 322875, 'AGAGG': 372622, 'AGTAA': 53695, 'AGTAT': 93468, 'AGTAC': 165574, 'AGTAG': 67203, 'AGTTA': 67136, 'AGTTT': 157416, 'AGTTC': 236889, 'AGTTG': 108199, 'AGTCA': 140476, 'AGTCT': 124839, 'AGTCC': 224813, 'AGTCG': 83353, 'AGTGA': 211474, 'AGTGT': 136989, 'AGTGC': 213180, 'AGTGG': 283509, 'AGCAA': 210926, 'AGCAT': 141820, 'AGCAC': 272710, 'AGCAG': 531880, 'AGCTA': 100180, 'AGCTT': 163036, 'AGCTC': 320105, 'AGCTG': 596867, 'AGCCA': 403395, 'AGCCT': 376733, 'AGCCC': 511681, 'AGCCG': 272569, 'AGCGA': 136426, 'AGCGT': 109031, 'AGCGC': 233743, 'AGCGG': 313609, 'AGGAA': 305726, 'AGGAT': 203300, 'AGGAC': 379790, 'AGGAG': 702706, 'AGGTA': 78713, 'AGGTT': 126804, 'AGGTC': 213569, 'AGGTG': 384053, 'AGGCA': 269257, 'AGGCT': 281684, 'AGGCC': 524727, 'AGGCG': 230823, 'AGGGA': 226437, 'AGGGT': 181460, 'AGGGC': 373169, 'AGGGG': 277300, 'TAAAA': 122923, 'TAAAT': 65614, 'TAAAC': 63152, 'TAAAG': 119234, 'TAATA': 30999, 'TAATT': 67137, 'TAATC': 42925, 'TAATG': 70589, 'TAACA': 72162, 'TAACT': 65006, 'TAACC': 57819, 'TAACG': 20427, 'TAAGA': 85301, 'TAAGT': 45297, 'TAAGC': 57747, 'TAAGG': 61380, 'TATAA': 47838, 'TATAT': 61171, 'TATAC': 38573, 'TATAG': 32105, 'TATTA': 50874, 'TATTT': 149547, 'TATTC': 65247, 'TATTG': 69249, 'TATCA': 69227, 'TATCT': 91682, 'TATCC': 72909, 'TATCG': 23997, 'TATGA': 114405, 'TATGT': 91202, 'TATGC': 88201, 'TATGG': 107803, 'TACAA': 177896, 'TACAT': 106340, 'TACAC': 100165, 'TACAG': 139792, 'TACTA': 62442, 'TACTT': 94473, 'TACTC': 70965, 'TACTG': 122104, 'TACCA': 144824, 'TACCT': 151976, 'TACCC': 129520, 'TACCG': 103341, 'TACGA': 104838, 'TACGT': 42415, 'TACGC': 69410, 'TACGG': 80162, 'TAGAA': 68647, 'TAGAT': 41334, 'TAGAC': 52153, 'TAGAG': 90209, 'TAGTA': 30867, 'TAGTT': 40470, 'TAGTC': 42247, 'TAGTG': 64459, 'TAGCA': 57770, 'TAGCT': 68612, 'TAGCC': 77649, 'TAGCG': 26589, 'TAGGA': 70911, 'TAGGT': 35847, 'TAGGC': 51429, 'TAGGG': 55225, 'TTAAA': 117330, 'TTAAT': 65736, 'TTAAC': 64066, 'TTAAG': 74649, 'TTATA': 52960, 'TTATT': 112258, 'TTATC': 58771, 'TTATG': 81729, 'TTACA': 89643, 'TTACT': 79381, 'TTACC': 70144, 'TTACG': 22253, 'TTAGA': 51257, 'TTAGT': 44684, 'TTAGC': 61151, 'TTAGG': 49274, 'TTTAA': 120398, 'TTTAT': 132832, 'TTTAC': 67005, 'TTTAG': 62678, 'TTTTA': 162270, 'TTTTT': 361927, 'TTTTC': 167216, 'TTTTG': 199674, 'TTTCA': 151855, 'TTTCT': 214684, 'TTTCC': 203400, 'TTTCG': 59515, 'TTTGA': 191851, 'TTTGT': 190988, 'TTTGC': 165935, 'TTTGG': 215644, 'TTCAA': 212089, 'TTCAT': 161991, 'TTCAC': 185635, 'TTCAG': 211755, 'TTCTA': 113102, 'TTCTT': 200424, 'TTCTC': 260639, 'TTCTG': 254844, 'TTCCA': 248996, 'TTCCT': 333461, 'TTCCC': 268010, 'TTCCG': 131134, 'TTCGA': 119103, 'TTCGT': 78229, 'TTCGC': 74278, 'TTCGG': 112872, 'TTGAA': 149465, 'TTGAT': 100336, 'TTGAC': 136183, 'TTGAG': 171427, 'TTGTA': 82905, 'TTGTT': 123968, 'TTGTC': 125553, 'TTGTG': 196036, 'TTGCA': 130091, 'TTGCT': 148703, 'TTGCC': 190593, 'TTGCG': 56493, 'TTGGA': 167582, 'TTGGT': 106606, 'TTGGC': 179561, 'TTGGG': 192517, 'TCAAA': 168768, 'TCAAT': 80054, 'TCAAC': 225763, 'TCAAG': 346740, 'TCATA': 53932, 'TCATT': 116996, 'TCATC': 286507, 'TCATG': 233457, 'TCACA': 156956, 'TCACT': 170601, 'TCACC': 327270, 'TCACG': 108809, 'TCAGA': 211551, 'TCAGT': 149787, 'TCAGC': 278590, 'TCAGG': 231305, 'TCTAA': 47976, 'TCTAT': 75785, 'TCTAC': 186026, 'TCTAG': 70111, 'TCTTA': 68775, 'TCTTT': 200579, 'TCTTC': 317472, 'TCTTG': 146057, 'TCTCA': 201496, 'TCTCT': 238274, 'TCTCC': 359024, 'TCTCG': 110369, 'TCTGA': 184957, 'TCTGT': 221855, 'TCTGC': 298741, 'TCTGG': 348964, 'TCCAA': 231251, 'TCCAT': 164025, 'TCCAC': 300267, 'TCCAG': 428443, 'TCCTA': 116414, 'TCCTT': 203583, 'TCCTC': 386937, 'TCCTG': 544875, 'TCCCA': 328966, 'TCCCT': 301318, 'TCCCC': 448521, 'TCCCG': 220425, 'TCCGA': 124928, 'TCCGT': 101075, 'TCCGC': 228046, 'TCCGG': 219684, 'TCGAA': 58544, 'TCGAT': 43796, 'TCGAC': 112562, 'TCGAG': 171333, 'TCGTA': 29443, 'TCGTT': 32276, 'TCGTC': 100052, 'TCGTG': 156285, 'TCGCA': 66313, 'TCGCT': 99880, 'TCGCC': 165389, 'TCGCG': 75182, 'TCGGA': 122012, 'TCGGT': 75022, 'TCGGC': 188139, 'TCGGG': 172118, 'TGAAA': 185519, 'TGAAT': 113572, 'TGAAC': 200341, 'TGAAG': 447217, 'TGATA': 63686, 'TGATT': 99753, 'TGATC': 167617, 'TGATG': 269935, 'TGACA': 201569, 'TGACT': 146942, 'TGACC': 320657, 'TGACG': 123387, 'TGAGA': 214317, 'TGAGT': 148044, 'TGAGC': 305925, 'TGAGG': 358996, 'TGTAA': 95492, 'TGTAT': 88996, 'TGTAC': 120612, 'TGTAG': 72385, 'TGTTA': 73608, 'TGTTT': 186554, 'TGTTC': 175719, 'TGTTG': 148017, 'TGTCA': 171253, 'TGTCT': 188934, 'TGTCC': 281990, 'TGTCG': 85436, 'TGTGA': 234642, 'TGTGT': 217204, 'TGTGC': 269575, 'TGTGG': 376301, 'TGCAA': 161940, 'TGCAT': 119594, 'TGCAC': 207852, 'TGCAG': 479521, 'TGCTA': 86147, 'TGCTT': 167382, 'TGCTC': 245967, 'TGCTG': 600066, 'TGCCA': 329943, 'TGCCT': 359486, 'TGCCC': 489096, 'TGCCG': 221796, 'TGCGA': 92787, 'TGCGT': 91966, 'TGCGC': 205270, 'TGCGG': 221189, 'TGGAA': 247125, 'TGGAT': 199582, 'TGGAC': 391252, 'TGGAG': 642514, 'TGGTA': 88069, 'TGGTT': 130442, 'TGGTC': 238490, 'TGGTG': 452989, 'TGGCA': 289466, 'TGGCT': 345635, 'TGGCC': 587090, 'TGGCG': 247616, 'TGGGA': 322525, 'TGGGT': 211773, 'TGGGC': 471924, 'TGGGG': 424512, 'CAAAA': 212694, 'CAAAT': 132512, 'CAAAC': 160895, 'CAAAG': 270337, 'CAATA': 59586, 'CAATT': 70251, 'CAATC': 76847, 'CAATG': 149615, 'CAACA': 303042, 'CAACT': 160078, 'CAACC': 262240, 'CAACG': 154134, 'CAAGA': 498911, 'CAAGT': 244751, 'CAAGC': 342737, 'CAAGG': 479891, 'CATAA': 55324, 'CATAT': 49625, 'CATAC': 64102, 'CATAG': 53140, 'CATTA': 69653, 'CATTT': 129373, 'CATTC': 127512, 'CATTG': 175874, 'CATCA': 340850, 'CATCT': 279426, 'CATCC': 360911, 'CATCG': 210385, 'CATGA': 242562, 'CATGT': 183101, 'CATGC': 222141, 'CATGG': 358079, 'CACAA': 185402, 'CACAT': 140180, 'CACAC': 214960, 'CACAG': 338026, 'CACTA': 90470, 'CACTT': 156500, 'CACTC': 169802, 'CACTG': 298455, 'CACCA': 435738, 'CACCT': 370108, 'CACCC': 455871, 'CACCG': 234947, 'CACGA': 115595, 'CACGT': 105404, 'CACGC': 226838, 'CACGG': 210201, 'CAGAA': 338510, 'CAGAT': 241050, 'CAGAC': 283612, 'CAGAG': 424144, 'CAGTA': 112350, 'CAGTT': 180313, 'CAGTC': 170550, 'CAGTG': 357831, 'CAGCA': 509355, 'CAGCT': 451409, 'CAGCC': 708752, 'CAGCG': 324428, 'CAGGA': 424910, 'CAGGT': 276015, 'CAGGC': 458734, 'CAGGG': 381262, 'CTAAA': 82634, 'CTAAT': 44455, 'CTAAC': 43947, 'CTAAG': 76809, 'CTATA': 44188, 'CTATT': 76218, 'CTATC': 81905, 'CTATG': 155477, 'CTACA': 223162, 'CTACT': 120913, 'CTACC': 247837, 'CTACG': 181016, 'CTAGA': 70559, 'CTAGT': 45387, 'CTAGC': 63249, 'CTAGG': 65625, 'CTTAA': 68988, 'CTTAT': 66863, 'CTTAC': 59898, 'CTTAG': 64533, 'CTTTA': 74762, 'CTTTT': 199062, 'CTTTC': 196989, 'CTTTG': 257692, 'CTTCA': 333477, 'CTTCT': 315282, 'CTTCC': 468040, 'CTTCG': 178814, 'CTTGA': 137006, 'CTTGT': 128363, 'CTTGC': 155250, 'CTTGG': 200912, 'CTCAA': 244767, 'CTCAT': 198032, 'CTCAC': 256684, 'CTCAG': 333478, 'CTCTA': 106863, 'CTCTT': 213826, 'CTCTC': 277890, 'CTCTG': 381264, 'CTCCA': 430123, 'CTCCT': 429264, 'CTCCC': 515128, 'CTCCG': 256697, 'CTCGA': 83493, 'CTCGT': 78932, 'CTCGC': 146462, 'CTCGG': 237753, 'CTGAA': 312108, 'CTGAT': 184304, 'CTGAC': 255050, 'CTGAG': 379286, 'CTGTA': 133599, 'CTGTT': 180919, 'CTGTC': 271439, 'CTGTG': 411261, 'CTGCA': 442958, 'CTGCT': 518725, 'CTGCC': 663325, 'CTGCG': 308653, 'CTGGA': 640286, 'CTGGT': 348203, 'CTGGC': 584932, 'CTGGG': 639658, 'CCAAA': 224527, 'CCAAT': 105188, 'CCAAC': 286785, 'CCAAG': 511678, 'CCATA': 78077, 'CCATT': 150408, 'CCATC': 396133, 'CCATG': 362772, 'CCACA': 325174, 'CCACT': 249064, 'CCACC': 604950, 'CCACG': 264523, 'CCAGA': 461912, 'CCAGT': 279226, 'CCAGC': 727155, 'CCAGG': 618775, 'CCTAA': 77009, 'CCTAT': 112075, 'CCTAC': 240180, 'CCTAG': 85974, 'CCTTA': 83571, 'CCTTT': 203793, 'CCTTC': 428824, 'CCTTG': 216115, 'CCTCA': 403570, 'CCTCT': 350792, 'CCTCC': 637916, 'CCTCG': 230349, 'CCTGA': 383762, 'CCTGT': 300734, 'CCTGC': 716865, 'CCTGG': 885874, 'CCCAA': 309461, 'CCCAT': 232999, 'CCCAC': 464887, 'CCCAG': 742776, 'CCCTA': 150507, 'CCCTT': 268362, 'CCCTC': 460906, 'CCCTG': 693751, 'CCCCA': 624453, 'CCCCT': 499833, 'CCCCC': 964023, 'CCCCG': 445294, 'CCCGA': 238506, 'CCCGT': 157578, 'CCCGC': 399806, 'CCCGG': 448316, 'CCGAA': 96906, 'CCGAT': 67637, 'CCGAC': 204750, 'CCGAG': 341620, 'CCGTA': 44881, 'CCGTT': 62491, 'CCGTC': 177032, 'CCGTG': 243778, 'CCGCA': 267607, 'CCGCT': 259830, 'CCGCC': 572959, 'CCGCG': 266061, 'CCGGA': 260965, 'CCGGT': 99777, 'CCGGC': 403548, 'CCGGG': 450548, 'CGAAA': 50638, 'CGAAT': 49534, 'CGAAC': 62608, 'CGAAG': 107839, 'CGATA': 26705, 'CGATT': 47712, 'CGATC': 48575, 'CGATG': 117603, 'CGACA': 150382, 'CGACT': 106554, 'CGACC': 186553, 'CGACG': 139650, 'CGAGA': 229964, 'CGAGT': 140261, 'CGAGC': 241691, 'CGAGG': 370916, 'CGTAA': 28325, 'CGTAT': 22165, 'CGTAC': 47169, 'CGTAG': 31720, 'CGTTA': 15092, 'CGTTT': 50940, 'CGTTC': 60822, 'CGTTG': 60319, 'CGTCA': 145010, 'CGTCT': 139602, 'CGTCC': 176157, 'CGTCG': 70543, 'CGTGA': 127999, 'CGTGT': 119433, 'CGTGC': 219361, 'CGTGG': 329699, 'CGCAA': 159515, 'CGCAT': 125396, 'CGCAC': 145665, 'CGCAG': 215719, 'CGCTA': 89462, 'CGCTT': 123145, 'CGCTC': 180601, 'CGCTG': 353341, 'CGCCA': 313382, 'CGCCT': 303374, 'CGCCC': 389975, 'CGCCG': 314964, 'CGCGA': 104460, 'CGCGT': 82392, 'CGCGC': 202850, 'CGCGG': 236978, 'CGGAA': 182232, 'CGGAT': 92657, 'CGGAC': 180332, 'CGGAG': 344079, 'CGGTA': 37580, 'CGGTT': 58264, 'CGGTC': 98450, 'CGGTG': 168433, 'CGGCA': 267692, 'CGGCT': 255831, 'CGGCC': 492414, 'CGGCG': 338497, 'CGGGA': 284266, 'CGGGT': 130442, 'CGGGC': 352919, 'CGGGG': 354867, 'GAAAA': 229667, 'GAAAT': 141683, 'GAAAC': 190565, 'GAAAG': 228048, 'GAATA': 73778, 'GAATT': 105789, 'GAATC': 95344, 'GAATG': 159769, 'GAACA': 216961, 'GAACT': 172009, 'GAACC': 215511, 'GAACG': 126983, 'GAAGA': 566889, 'GAAGT': 219597, 'GAAGC': 415108, 'GAAGG': 423498, 'GATAA': 67295, 'GATAT': 60724, 'GATAC': 58396, 'GATAG': 47748, 'GATTA': 61557, 'GATTT': 116751, 'GATTC': 122960, 'GATTG': 108016, 'GATCA': 179054, 'GATCT': 142323, 'GATCC': 239416, 'GATCG': 96582, 'GATGA': 354565, 'GATGT': 161760, 'GATGC': 211677, 'GATGG': 292399, 'GACAA': 261100, 'GACAT': 211401, 'GACAC': 193401, 'GACAG': 294535, 'GACTA': 84346, 'GACTT': 162032, 'GACTC': 210857, 'GACTG': 213245, 'GACCA': 372346, 'GACCT': 297746, 'GACCC': 377787, 'GACCG': 179748, 'GACGA': 174973, 'GACGT': 116139, 'GACGC': 196752, 'GACGG': 213186, 'GAGAA': 451001, 'GAGAT': 249912, 'GAGAC': 283275, 'GAGAG': 324129, 'GAGTA': 109224, 'GAGTT': 167837, 'GAGTC': 183838, 'GAGTG': 237127, 'GAGCA': 338953, 'GAGCT': 373185, 'GAGCC': 464086, 'GAGCG': 281343, 'GAGGA': 700058, 'GAGGT': 272408, 'GAGGC': 500622, 'GAGGG': 377177, 'GTAAA': 72040, 'GTAAT': 53908, 'GTAAC': 56294, 'GTAAG': 46789, 'GTATA': 31383, 'GTATT': 73057, 'GTATC': 57120, 'GTATG': 102250, 'GTACA': 128498, 'GTACT': 86126, 'GTACC': 144424, 'GTACG': 63962, 'GTAGA': 75742, 'GTAGT': 45394, 'GTAGC': 65512, 'GTAGG': 51985, 'GTTAA': 54333, 'GTTAT': 46079, 'GTTAC': 55874, 'GTTAG': 44806, 'GTTTA': 61800, 'GTTTT': 143464, 'GTTTC': 128411, 'GTTTG': 189708, 'GTTCA': 166937, 'GTTCT': 177301, 'GTTCC': 188960, 'GTTCG': 91521, 'GTTGA': 84588, 'GTTGT': 102635, 'GTTGC': 105397, 'GTTGG': 144735, 'GTCAA': 152508, 'GTCAT': 144303, 'GTCAC': 175224, 'GTCAG': 193984, 'GTCTA': 77064, 'GTCTT': 149050, 'GTCTC': 201437, 'GTCTG': 227736, 'GTCCA': 235288, 'GTCCT': 258287, 'GTCCC': 315141, 'GTCCG': 131524, 'GTCGA': 63297, 'GTCGT': 82998, 'GTCGC': 100477, 'GTCGG': 122836, 'GTGAA': 257931, 'GTGAT': 133207, 'GTGAC': 228425, 'GTGAG': 217985, 'GTGTA': 80198, 'GTGTT': 157107, 'GTGTC': 173948, 'GTGTG': 276132, 'GTGCA': 235781, 'GTGCT': 268350, 'GTGCC': 316449, 'GTGCG': 168197, 'GTGGA': 426315, 'GTGGT': 280143, 'GTGGC': 433546, 'GTGGG': 377434, 'GCAAA': 169673, 'GCAAT': 86283, 'GCAAC': 163539, 'GCAAG': 350288, 'GCATA': 37806, 'GCATT': 113087, 'GCATC': 238351, 'GCATG': 195651, 'GCACA': 202721, 'GCACT': 173746, 'GCACC': 347793, 'GCACG': 170785, 'GCAGA': 363775, 'GCAGT': 237281, 'GCAGC': 651199, 'GCAGG': 455036, 'GCTAA': 67170, 'GCTAT': 99288, 'GCTAC': 194501, 'GCTAG': 59529, 'GCTTA': 54786, 'GCTTT': 171596, 'GCTTC': 310711, 'GCTTG': 142271, 'GCTCA': 287664, 'GCTCT': 250844, 'GCTCC': 403315, 'GCTCG': 125953, 'GCTGA': 389158, 'GCTGT': 316689, 'GCTGC': 686297, 'GCTGG': 715893, 'GCCAA': 324575, 'GCCAT': 342669, 'GCCAC': 430605, 'GCCAG': 514808, 'GCCTA': 142259, 'GCCTT': 284702, 'GCCTC': 487651, 'GCCTG': 606539, 'GCCCA': 491718, 'GCCCT': 496044, 'GCCCC': 706417, 'GCCCG': 415953, 'GCCGA': 243214, 'GCCGT': 169223, 'GCCGC': 508338, 'GCCGG': 373510, 'GCGAA': 63443, 'GCGAT': 66754, 'GCGAC': 134776, 'GCGAG': 240231, 'GCGTA': 25792, 'GCGTT': 53978, 'GCGTC': 150842, 'GCGTG': 215862, 'GCGCA': 193128, 'GCGCT': 220308, 'GCGCC': 361927, 'GCGCG': 201397, 'GCGGA': 253662, 'GCGGT': 112243, 'GCGGC': 540940, 'GCGGG': 338092, 'GGAAA': 223070, 'GGAAT': 127339, 'GGAAC': 197473, 'GGAAG': 438539, 'GGATA': 68135, 'GGATT': 123595, 'GGATC': 155978, 'GGATG': 300262, 'GGACA': 360233, 'GGACT': 251417, 'GGACC': 431612, 'GGACG': 251829, 'GGAGA': 546259, 'GGAGT': 240724, 'GGAGC': 583937, 'GGAGG': 745534, 'GGTAA': 51271, 'GGTAT': 60077, 'GGTAC': 90872, 'GGTAG': 67551, 'GGTTA': 44746, 'GGTTT': 129597, 'GGTTC': 150416, 'GGTTG': 119311, 'GGTCA': 206282, 'GGTCT': 199187, 'GGTCC': 260005, 'GGTCG': 129883, 'GGTGA': 262704, 'GGTGT': 213256, 'GGTGC': 287225, 'GGTGG': 528852, 'GGCAA': 238059, 'GGCAT': 198253, 'GGCAC': 270510, 'GGCAG': 480054, 'GGCTA': 144025, 'GGCTT': 226143, 'GGCTC': 322278, 'GGCTG': 558183, 'GGCCA': 561322, 'GGCCT': 481173, 'GGCCC': 732132, 'GGCCG': 483016, 'GGCGA': 169729, 'GGCGT': 162803, 'GGCGC': 334863, 'GGCGG': 472405, 'GGGAA': 253743, 'GGGAT': 153231, 'GGGAC': 345542, 'GGGAG': 426358, 'GGGTA': 65911, 'GGGTT': 127590, 'GGGTC': 244444, 'GGGTG': 286377, 'GGGCA': 358904, 'GGGCT': 363649, 'GGGCC': 658268, 'GGGCG': 317661, 'GGGGA': 346690, 'GGGGT': 200421, 'GGGGC': 503429, 'GGGGG': 371829}, 'overrepresented_sequences': {}} {'total_reads': 3526970, 'total_bases': 204365871, 'q20_bases': 198574187, 'q30_bases': 191444360, 'total_cycles': 58, 'quality_curves': {'A': [37.1908, 37.189, 37.1974, 37.2112, 37.1983, 37.2327, 37.1838, 37.1752, 37.1965, 37.1827, 37.1749, 37.1448, 37.1149, 37.0357, 37.0629, 37.0212, 36.9807, 36.9558, 36.9666, 36.997, 36.9391, 36.9311, 36.8998, 36.7932, 36.6827, 36.5538, 36.4323, 36.3821, 36.2469, 36.1706, 36.16, 36.0545, 35.9889, 35.9463, 35.9157, 35.9155, 35.7686, 35.7001, 35.6714, 35.6277, 35.4983, 35.3562, 35.3525, 35.3164, 35.2477, 35.1346, 35.116, 35.0363, 35.015, 34.8042, 34.6779, 34.5815, 34.5253, 34.4827, 34.2557, 34.1391, 33.9851, 33.6704], 'T': [37.4368, 37.4253, 37.4471, 37.4148, 37.3898, 37.3724, 37.3355, 37.3239, 37.327, 37.3192, 37.2886, 37.2706, 37.2704, 37.2046, 37.2698, 37.2732, 37.2251, 37.1643, 37.1595, 37.1543, 37.1531, 37.1313, 37.1202, 37.0592, 37.0037, 36.8922, 36.7844, 36.7558, 36.6725, 36.6116, 36.6085, 36.5234, 36.4533, 36.4157, 36.3919, 36.3735, 36.2841, 36.2038, 36.1526, 36.1, 35.9873, 35.8895, 35.8828, 35.8337, 35.7526, 35.6512, 35.635, 35.5608, 35.502, 35.348, 35.1901, 35.1285, 34.9876, 34.9734, 34.7544, 34.6579, 34.5201, 34.207], 'C': [37.3984, 37.4233, 37.3876, 37.3942, 37.4205, 37.3848, 37.3938, 37.3924, 37.3708, 37.3913, 37.3899, 37.3775, 37.3353, 37.3445, 37.3174, 37.2928, 37.2984, 37.2935, 37.2685, 37.2561, 37.2214, 37.2295, 37.1975, 37.1207, 37.0383, 36.9874, 36.8649, 36.8279, 36.7666, 36.68, 36.6824, 36.6311, 36.5581, 36.5598, 36.5381, 36.4974, 36.4452, 36.3765, 36.3458, 36.3045, 36.2359, 36.1589, 36.125, 36.1161, 36.0359, 35.9496, 35.9403, 35.9068, 35.8266, 35.7105, 35.6113, 35.4825, 35.4048, 35.3371, 35.2206, 35.0926, 34.9109, 34.6762], 'G': [37.2361, 37.2296, 37.2203, 37.226, 37.223, 37.2282, 37.2073, 37.2198, 37.2159, 37.2188, 37.2186, 37.1844, 37.1683, 37.1101, 37.0928, 37.0735, 37.0785, 37.0513, 37.0698, 37.0725, 37.009, 37.0005, 36.9616, 36.8948, 36.8369, 36.7558, 36.6563, 36.6175, 36.5404, 36.4534, 36.4775, 36.3776, 36.3165, 36.311, 36.2881, 36.3137, 36.2115, 36.1389, 36.1004, 36.0872, 35.9662, 35.896, 35.8488, 35.8421, 35.7667, 35.6592, 35.6199, 35.5334, 35.4877, 35.3795, 35.221, 35.1342, 34.9923, 34.9872, 34.8475, 34.6932, 34.4876, 34.248], 'mean': [37.3184, 37.3195, 37.313, 37.311, 37.3097, 37.3041, 37.2819, 37.2822, 37.2785, 37.2814, 37.2729, 37.2489, 37.2262, 37.1816, 37.1885, 37.1679, 37.1527, 37.1241, 37.1244, 37.1274, 37.0857, 37.079, 37.0509, 36.9735, 36.9003, 36.8124, 36.698, 36.6611, 36.5766, 36.4951, 36.5016, 36.4184, 36.3486, 36.3317, 36.3099, 36.2983, 36.2057, 36.1348, 36.0936, 36.06, 35.9555, 35.8595, 35.8354, 35.817, 35.736, 35.6335, 35.6168, 35.5442, 35.493, 35.3529, 35.216, 35.1222, 35.0184, 34.9824, 34.8145, 34.6919, 34.5137, 34.247]}, 'content_curves': {'A': [0.202517, 0.19558, 0.202513, 0.202608, 0.198494, 0.204364, 0.201883, 0.197371, 0.204489, 0.198972, 0.200586, 0.20516, 0.204133, 0.198284, 0.205667, 0.202544, 0.199403, 0.204598, 0.201149, 0.196629, 0.203146, 0.201351, 0.197172, 0.202234, 0.197332, 0.196112, 0.201193, 0.196507, 0.194652, 0.199836, 0.197164, 0.195706, 0.201102, 0.198319, 0.194656, 0.201219, 0.198693, 0.195856, 0.203382, 0.198047, 0.196722, 0.200802, 0.198599, 0.193533, 0.199588, 0.199959, 0.194011, 0.201376, 0.195619, 0.196179, 0.199316, 0.196375, 0.195732, 0.199996, 0.198879, 0.195433, 0.200174, 0.197419], 'T': [0.244844, 0.228589, 0.234506, 0.227847, 0.222719, 0.229652, 0.226695, 0.22528, 0.230391, 0.232229, 0.222754, 0.228097, 0.230271, 0.220264, 0.224186, 0.225429, 0.219382, 0.22436, 0.227249, 0.222816, 0.224365, 0.226223, 0.221198, 0.224095, 0.224955, 0.220693, 0.224588, 0.226645, 0.222283, 0.225, 0.225843, 0.221174, 0.226302, 0.22796, 0.224147, 0.226441, 0.22625, 0.221752, 0.220967, 0.224963, 0.219964, 0.225471, 0.225666, 0.222735, 0.224606, 0.227555, 0.221922, 0.225458, 0.227126, 0.223024, 0.221629, 0.226976, 0.218421, 0.220992, 0.22371, 0.217053, 0.221573, 0.219648], 'C': [0.260904, 0.274241, 0.264498, 0.267326, 0.275723, 0.267243, 0.269809, 0.27659, 0.269393, 0.272073, 0.278915, 0.274058, 0.271224, 0.279241, 0.276551, 0.273465, 0.279945, 0.276499, 0.276505, 0.280293, 0.275984, 0.274641, 0.281378, 0.276483, 0.279444, 0.285355, 0.278064, 0.277954, 0.283096, 0.276245, 0.278629, 0.283099, 0.277611, 0.27804, 0.284007, 0.278454, 0.28148, 0.283812, 0.281013, 0.280583, 0.284233, 0.278828, 0.280921, 0.286621, 0.28241, 0.278799, 0.285173, 0.280334, 0.27895, 0.2818, 0.282156, 0.280796, 0.287374, 0.283319, 0.28284, 0.287066, 0.282167, 0.285022], 'G': [0.291735, 0.30159, 0.29848, 0.302218, 0.303063, 0.298741, 0.301613, 0.300759, 0.295705, 0.296713, 0.297738, 0.292686, 0.294372, 0.302212, 0.293595, 0.298561, 0.30127, 0.294543, 0.295097, 0.300261, 0.296505, 0.297786, 0.300251, 0.297187, 0.298268, 0.29784, 0.296155, 0.298895, 0.299969, 0.298919, 0.298364, 0.300021, 0.294984, 0.295681, 0.29719, 0.293885, 0.293577, 0.298581, 0.294638, 0.296407, 0.299081, 0.294899, 0.294812, 0.297111, 0.293396, 0.293687, 0.298894, 0.292832, 0.298304, 0.298998, 0.296899, 0.295853, 0.298474, 0.295693, 0.294571, 0.300447, 0.296087, 0.29791], 'N': [0, 0, 3.96941e-06, 0, 0, 0, 0, 0, 2.18318e-05, 1.24753e-05, 7.37177e-06, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1.41881e-06, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 8.57378e-07], 'GC': [0.55264, 0.575831, 0.562978, 0.569544, 0.578787, 0.565984, 0.571422, 0.577349, 0.565098, 0.568786, 0.576653, 0.566743, 0.565596, 0.581452, 0.570146, 0.572026, 0.581215, 0.571042, 0.571602, 0.580555, 0.572489, 0.572426, 0.581629, 0.573671, 0.577713, 0.583195, 0.574219, 0.576849, 0.583065, 0.575164, 0.576993, 0.58312, 0.572596, 0.57372, 0.581197, 0.572339, 0.575057, 0.582393, 0.575651, 0.576991, 0.583313, 0.573727, 0.575733, 0.583731, 0.575807, 0.572486, 0.584067, 0.573166, 0.577255, 0.580797, 0.579055, 0.576649, 0.585848, 0.579012, 0.577411, 0.587513, 0.578253, 0.582932]}, 'kmer_count': {'AAAAA': 368712, 'AAAAT': 189241, 'AAAAC': 143027, 'AAAAG': 183668, 'AAATA': 159186, 'AAATT': 125966, 'AAATC': 115234, 'AAATG': 137773, 'AAACA': 173907, 'AAACT': 153555, 'AAACC': 127426, 'AAACG': 50354, 'AAAGA': 164231, 'AAAGT': 144031, 'AAAGC': 163468, 'AAAGG': 181917, 'AATAA': 125143, 'AATAT': 79303, 'AATAC': 67072, 'AATAG': 69846, 'AATTA': 71063, 'AATTT': 129499, 'AATTC': 104473, 'AATTG': 70948, 'AATCA': 95437, 'AATCT': 142588, 'AATCC': 113094, 'AATCG': 39824, 'AATGA': 115136, 'AATGT': 113646, 'AATGC': 112682, 'AATGG': 158851, 'AACAA': 122646, 'AACAT': 116522, 'AACAC': 134135, 'AACAG': 166638, 'AACTA': 43370, 'AACTT': 178022, 'AACTC': 153452, 'AACTG': 164023, 'AACCA': 115518, 'AACCT': 108659, 'AACCC': 121721, 'AACCG': 45163, 'AACGA': 27059, 'AACGT': 36706, 'AACGC': 45726, 'AACGG': 59057, 'AAGAA': 185348, 'AAGAT': 137943, 'AAGAC': 114846, 'AAGAG': 191842, 'AAGTA': 93315, 'AAGTT': 135606, 'AAGTC': 150025, 'AAGTG': 165820, 'AAGCA': 148061, 'AAGCT': 156307, 'AAGCC': 222244, 'AAGCG': 107532, 'AAGGA': 197424, 'AAGGT': 160929, 'AAGGC': 260524, 'AAGGG': 249029, 'ATAAA': 135775, 'ATAAT': 63784, 'ATAAC': 46086, 'ATAAG': 62715, 'ATATA': 61018, 'ATATT': 76430, 'ATATC': 50406, 'ATATG': 51601, 'ATACA': 95471, 'ATACT': 88659, 'ATACC': 50612, 'ATACG': 20377, 'ATAGA': 75995, 'ATAGT': 72646, 'ATAGC': 85616, 'ATAGG': 88557, 'ATTAA': 65707, 'ATTAT': 48370, 'ATTAC': 58932, 'ATTAG': 44607, 'ATTTA': 66510, 'ATTTT': 160978, 'ATTTC': 136803, 'ATTTG': 133222, 'ATTCA': 114638, 'ATTCT': 140854, 'ATTCC': 120995, 'ATTCG': 42868, 'ATTGA': 85364, 'ATTGT': 74288, 'ATTGC': 79140, 'ATTGG': 102711, 'ATCAA': 91305, 'ATCAT': 163278, 'ATCAC': 122658, 'ATCAG': 168569, 'ATCTA': 40278, 'ATCTT': 292745, 'ATCTC': 231372, 'ATCTG': 225676, 'ATCCA': 186738, 'ATCCT': 175888, 'ATCCC': 124570, 'ATCCG': 78552, 'ATCGA': 36625, 'ATCGT': 52519, 'ATCGC': 52259, 'ATCGG': 53177, 'ATGAA': 166709, 'ATGAT': 172322, 'ATGAC': 126761, 'ATGAG': 206764, 'ATGTA': 104542, 'ATGTT': 194393, 'ATGTC': 189195, 'ATGTG': 133344, 'ATGCA': 109768, 'ATGCT': 135653, 'ATGCC': 185093, 'ATGCG': 120568, 'ATGGA': 162557, 'ATGGT': 208182, 'ATGGC': 268863, 'ATGGG': 222893, 'ACAAA': 190098, 'ACAAT': 107220, 'ACAAC': 82403, 'ACAAG': 129993, 'ACATA': 85935, 'ACATT': 130507, 'ACATC': 146437, 'ACATG': 155984, 'ACACA': 200277, 'ACACT': 117623, 'ACACC': 184960, 'ACACG': 109980, 'ACAGA': 193381, 'ACAGT': 139253, 'ACAGC': 281736, 'ACAGG': 292225, 'ACTAA': 42205, 'ACTAT': 39391, 'ACTAC': 35141, 'ACTAG': 40709, 'ACTTA': 41581, 'ACTTT': 145035, 'ACTTC': 210819, 'ACTTG': 228247, 'ACTCA': 138806, 'ACTCT': 134150, 'ACTCC': 195301, 'ACTCG': 127997, 'ACTGA': 132681, 'ACTGT': 148691, 'ACTGC': 209878, 'ACTGG': 248035, 'ACCAA': 95172, 'ACCAT': 141904, 'ACCAC': 250748, 'ACCAG': 307074, 'ACCTA': 34552, 'ACCTT': 181249, 'ACCTC': 234636, 'ACCTG': 234685, 'ACCCA': 185308, 'ACCCT': 151711, 'ACCCC': 174857, 'ACCCG': 102069, 'ACCGA': 63932, 'ACCGT': 67432, 'ACCGC': 89555, 'ACCGG': 78553, 'ACGAA': 59385, 'ACGAT': 66955, 'ACGAC': 57413, 'ACGAG': 78118, 'ACGTA': 39968, 'ACGTT': 88433, 'ACGTC': 106319, 'ACGTG': 99966, 'ACGCA': 76044, 'ACGCT': 84266, 'ACGCC': 130786, 'ACGCG': 61052, 'ACGGA': 89577, 'ACGGT': 99618, 'ACGGC': 135954, 'ACGGG': 135434, 'AGAAA': 209588, 'AGAAT': 107947, 'AGAAC': 157338, 'AGAAG': 296191, 'AGATA': 88431, 'AGATT': 81999, 'AGATC': 128155, 'AGATG': 241255, 'AGACA': 157814, 'AGACT': 104149, 'AGACC': 168113, 'AGACG': 108744, 'AGAGA': 216456, 'AGAGT': 120554, 'AGAGC': 212188, 'AGAGG': 349325, 'AGTAA': 77462, 'AGTAT': 60895, 'AGTAC': 75576, 'AGTAG': 111812, 'AGTTA': 59880, 'AGTTT': 144498, 'AGTTC': 148681, 'AGTTG': 151196, 'AGTCA': 142157, 'AGTCT': 150223, 'AGTCC': 213781, 'AGTCG': 92288, 'AGTGA': 163390, 'AGTGT': 125750, 'AGTGC': 165716, 'AGTGG': 247874, 'AGCAA': 128684, 'AGCAT': 149312, 'AGCAC': 242799, 'AGCAG': 422237, 'AGCTA': 65131, 'AGCTT': 251269, 'AGCTC': 324238, 'AGCTG': 421814, 'AGCCA': 314438, 'AGCCT': 255548, 'AGCCC': 336333, 'AGCCG': 213566, 'AGCGA': 90014, 'AGCGT': 132953, 'AGCGC': 181840, 'AGCGG': 214660, 'AGGAA': 316388, 'AGGAT': 208393, 'AGGAC': 232318, 'AGGAG': 419396, 'AGGTA': 144013, 'AGGTT': 168697, 'AGGTC': 262563, 'AGGTG': 369467, 'AGGCA': 317913, 'AGGCT': 338975, 'AGGCC': 410958, 'AGGCG': 259213, 'AGGGA': 295113, 'AGGGT': 251563, 'AGGGC': 448795, 'AGGGG': 490654, 'TAAAA': 156219, 'TAAAT': 91793, 'TAAAC': 65000, 'TAAAG': 73795, 'TAATA': 50912, 'TAATT': 72506, 'TAATC': 65740, 'TAATG': 70367, 'TAACA': 70911, 'TAACT': 68049, 'TAACC': 43481, 'TAACG': 12697, 'TAAGA': 66123, 'TAAGT': 47091, 'TAAGC': 55464, 'TAAGG': 80983, 'TATAA': 50001, 'TATAT': 51605, 'TATAC': 33725, 'TATAG': 43689, 'TATTA': 37913, 'TATTT': 129115, 'TATTC': 71301, 'TATTG': 71561, 'TATCA': 60080, 'TATCT': 72346, 'TATCC': 60764, 'TATCG': 23316, 'TATGA': 51088, 'TATGT': 50779, 'TATGC': 37560, 'TATGG': 74474, 'TACAA': 86417, 'TACAT': 81600, 'TACAC': 84283, 'TACAG': 129096, 'TACTA': 23976, 'TACTT': 125358, 'TACTC': 93572, 'TACTG': 99674, 'TACCA': 70360, 'TACCT': 57862, 'TACCC': 62624, 'TACCG': 32348, 'TACGA': 20103, 'TACGT': 27328, 'TACGC': 21279, 'TACGG': 43357, 'TAGAA': 101779, 'TAGAT': 77596, 'TAGAC': 61098, 'TAGAG': 107574, 'TAGTA': 62097, 'TAGTT': 72814, 'TAGTC': 81077, 'TAGTG': 87581, 'TAGCA': 77716, 'TAGCT': 88752, 'TAGCC': 117567, 'TAGCG': 68345, 'TAGGA': 113895, 'TAGGT': 100227, 'TAGGC': 110814, 'TAGGG': 132834, 'TTAAA': 121791, 'TTAAT': 82340, 'TTAAC': 53818, 'TTAAG': 70864, 'TTATA': 50332, 'TTATT': 88905, 'TTATC': 59743, 'TTATG': 54416, 'TTACA': 92943, 'TTACT': 51461, 'TTACC': 44294, 'TTACG': 27431, 'TTAGA': 41414, 'TTAGT': 56750, 'TTAGC': 54411, 'TTAGG': 76607, 'TTTAA': 123091, 'TTTAT': 103233, 'TTTAC': 68975, 'TTTAG': 65988, 'TTTTA': 122241, 'TTTTT': 429839, 'TTTTC': 227535, 'TTTTG': 176217, 'TTTCA': 170461, 'TTTCT': 324870, 'TTTCC': 215539, 'TTTCG': 40362, 'TTTGA': 142545, 'TTTGT': 199883, 'TTTGC': 159387, 'TTTGG': 194679, 'TTCAA': 154638, 'TTCAT': 205827, 'TTCAC': 240954, 'TTCAG': 287577, 'TTCTA': 76964, 'TTCTT': 480115, 'TTCTC': 426887, 'TTCTG': 325181, 'TTCCA': 228858, 'TTCCT': 294686, 'TTCCC': 235484, 'TTCCG': 140036, 'TTCGA': 51561, 'TTCGT': 42195, 'TTCGC': 45157, 'TTCGG': 71385, 'TTGAA': 189992, 'TTGAT': 205581, 'TTGAC': 156005, 'TTGAG': 228799, 'TTGTA': 156712, 'TTGTT': 210844, 'TTGTC': 240856, 'TTGTG': 172767, 'TTGCA': 151665, 'TTGCT': 202057, 'TTGCC': 211452, 'TTGCG': 135218, 'TTGGA': 216841, 'TTGGT': 239804, 'TTGGC': 307206, 'TTGGG': 293188, 'TCAAA': 181685, 'TCAAT': 142408, 'TCAAC': 81752, 'TCAAG': 122510, 'TCATA': 105717, 'TCATT': 124587, 'TCATC': 322496, 'TCATG': 219816, 'TCACA': 216848, 'TCACT': 195571, 'TCACC': 235705, 'TCACG': 117732, 'TCAGA': 173340, 'TCAGT': 163039, 'TCAGC': 345491, 'TCAGG': 349513, 'TCTAA': 51666, 'TCTAT': 58631, 'TCTAC': 68094, 'TCTAG': 70141, 'TCTTA': 80629, 'TCTTT': 256247, 'TCTTC': 515566, 'TCTTG': 478084, 'TCTCA': 191019, 'TCTCT': 291710, 'TCTCC': 483803, 'TCTCG': 210224, 'TCTGA': 187021, 'TCTGT': 221722, 'TCTGC': 335943, 'TCTGG': 446603, 'TCCAA': 155275, 'TCCAT': 210148, 'TCCAC': 375300, 'TCCAG': 569075, 'TCCTA': 64620, 'TCCTT': 372504, 'TCCTC': 627851, 'TCCTG': 392682, 'TCCCA': 281931, 'TCCCT': 197052, 'TCCCC': 290783, 'TCCCG': 238190, 'TCCGA': 89371, 'TCCGT': 129258, 'TCCGC': 176670, 'TCCGG': 212450, 'TCGAA': 109867, 'TCGAT': 102186, 'TCGAC': 42759, 'TCGAG': 64963, 'TCGTA': 86164, 'TCGTT': 78761, 'TCGTC': 160104, 'TCGTG': 109456, 'TCGCA': 86658, 'TCGCT': 111091, 'TCGCC': 133059, 'TCGCG': 75638, 'TCGGA': 94599, 'TCGGT': 96785, 'TCGGC': 191243, 'TCGGG': 206018, 'TGAAA': 133018, 'TGAAT': 106423, 'TGAAC': 151768, 'TGAAG': 318894, 'TGATA': 65255, 'TGATT': 75009, 'TGATC': 168669, 'TGATG': 327407, 'TGACA': 160846, 'TGACT': 121951, 'TGACC': 193621, 'TGACG': 144310, 'TGAGA': 196468, 'TGAGT': 136747, 'TGAGC': 281914, 'TGAGG': 377050, 'TGTAA': 89902, 'TGTAT': 75688, 'TGTAC': 114908, 'TGTAG': 210078, 'TGTTA': 73745, 'TGTTT': 175723, 'TGTTC': 204114, 'TGTTG': 283501, 'TGTCA': 199521, 'TGTCT': 227811, 'TGTCC': 332648, 'TGTCG': 132185, 'TGTGA': 149509, 'TGTGT': 182530, 'TGTGC': 194131, 'TGTGG': 311927, 'TGCAA': 121444, 'TGCAT': 149672, 'TGCAC': 200605, 'TGCAG': 392282, 'TGCTA': 55878, 'TGCTT': 232792, 'TGCTC': 307431, 'TGCTG': 479742, 'TGCCA': 263353, 'TGCCT': 248927, 'TGCCC': 327413, 'TGCCG': 229846, 'TGCGA': 55791, 'TGCGT': 91823, 'TGCGC': 159193, 'TGCGG': 258922, 'TGGAA': 255438, 'TGGAT': 182431, 'TGGAC': 208521, 'TGGAG': 416150, 'TGGTA': 130326, 'TGGTT': 192138, 'TGGTC': 327649, 'TGGTG': 408846, 'TGGCA': 320962, 'TGGCT': 353152, 'TGGCC': 498453, 'TGGCG': 255563, 'TGGGA': 310128, 'TGGGT': 300202, 'TGGGC': 450003, 'TGGGG': 630460, 'CAAAA': 196989, 'CAAAT': 118071, 'CAAAC': 174932, 'CAAAG': 225752, 'CAATA': 64520, 'CAATT': 80149, 'CAATC': 102939, 'CAATG': 169921, 'CAACA': 134974, 'CAACT': 100048, 'CAACC': 97565, 'CAACG': 50315, 'CAAGA': 123688, 'CAAGT': 125797, 'CAAGC': 121790, 'CAAGG': 210666, 'CATAA': 74163, 'CATAT': 57659, 'CATAC': 98992, 'CATAG': 141957, 'CATTA': 67968, 'CATTT': 132884, 'CATTC': 157123, 'CATTG': 134198, 'CATCA': 241136, 'CATCT': 304305, 'CATCC': 257504, 'CATCG': 93712, 'CATGA': 216638, 'CATGT': 190968, 'CATGC': 181536, 'CATGG': 287650, 'CACAA': 189703, 'CACAT': 193935, 'CACAC': 240034, 'CACAG': 365518, 'CACTA': 53684, 'CACTT': 172696, 'CACTC': 191496, 'CACTG': 332699, 'CACCA': 395435, 'CACCT': 340477, 'CACCC': 249195, 'CACCG': 135721, 'CACGA': 126449, 'CACGT': 169836, 'CACGC': 176039, 'CACGG': 215077, 'CAGAA': 245985, 'CAGAT': 175080, 'CAGAC': 187796, 'CAGAG': 332798, 'CAGTA': 99367, 'CAGTT': 169400, 'CAGTC': 189313, 'CAGTG': 272675, 'CAGCA': 494418, 'CAGCT': 532279, 'CAGCC': 487629, 'CAGCG': 286875, 'CAGGA': 488352, 'CAGGT': 407148, 'CAGGC': 536953, 'CAGGG': 644762, 'CTAAA': 57047, 'CTAAT': 37225, 'CTAAC': 44026, 'CTAAG': 55323, 'CTATA': 26154, 'CTATT': 57860, 'CTATC': 48517, 'CTATG': 49253, 'CTACA': 65493, 'CTACT': 56401, 'CTACC': 53524, 'CTACG': 27734, 'CTAGA': 67452, 'CTAGT': 27658, 'CTAGC': 53545, 'CTAGG': 87652, 'CTTAA': 76678, 'CTTAT': 48569, 'CTTAC': 38710, 'CTTAG': 71697, 'CTTTA': 116804, 'CTTTT': 200467, 'CTTTC': 202963, 'CTTTG': 232598, 'CTTCA': 410277, 'CTTCT': 575601, 'CTTCC': 397023, 'CTTCG': 79708, 'CTTGA': 341914, 'CTTGT': 306566, 'CTTGC': 313495, 'CTTGG': 483878, 'CTCAA': 159628, 'CTCAT': 237179, 'CTCAC': 181957, 'CTCAG': 342943, 'CTCTA': 78523, 'CTCTT': 306788, 'CTCTC': 266455, 'CTCTG': 385480, 'CTCCA': 568407, 'CTCCT': 637525, 'CTCCC': 341788, 'CTCCG': 245639, 'CTCGA': 142868, 'CTCGT': 211011, 'CTCGC': 204490, 'CTCGG': 293596, 'CTGAA': 178756, 'CTGAT': 116151, 'CTGAC': 170430, 'CTGAG': 308923, 'CTGTA': 130130, 'CTGTT': 182938, 'CTGTC': 274002, 'CTGTG': 316389, 'CTGCA': 400411, 'CTGCT': 472768, 'CTGCC': 437368, 'CTGCG': 186164, 'CTGGA': 392634, 'CTGGT': 364996, 'CTGGC': 455224, 'CTGGG': 702559, 'CCAAA': 195422, 'CCAAT': 79544, 'CCAAC': 123873, 'CCAAG': 190165, 'CCATA': 100554, 'CCATT': 138238, 'CCATC': 237439, 'CCATG': 302385, 'CCACA': 338207, 'CCACT': 233113, 'CCACC': 455941, 'CCACG': 290776, 'CCAGA': 300529, 'CCAGT': 229254, 'CCAGC': 618282, 'CCAGG': 775643, 'CCTAA': 44392, 'CCTAT': 49850, 'CCTAC': 42230, 'CCTAG': 63247, 'CCTTA': 54132, 'CCTTT': 169176, 'CCTTC': 382276, 'CCTTG': 425019, 'CCTCA': 318442, 'CCTCT': 340580, 'CCTCC': 641860, 'CCTCG': 309899, 'CCTGA': 206530, 'CCTGT': 215316, 'CCTGC': 401980, 'CCTGG': 565331, 'CCCAA': 169205, 'CCCAT': 204797, 'CCCAC': 324682, 'CCCAG': 550352, 'CCCTA': 52834, 'CCCTT': 221376, 'CCCTC': 315385, 'CCCTG': 347509, 'CCCCA': 374412, 'CCCCT': 249617, 'CCCCC': 305983, 'CCCCG': 272368, 'CCCGA': 138603, 'CCCGT': 150094, 'CCCGC': 253293, 'CCCGG': 352975, 'CCGAA': 85460, 'CCGAT': 78566, 'CCGAC': 86625, 'CCGAG': 177559, 'CCGTA': 74014, 'CCGTT': 100308, 'CCGTC': 178709, 'CCGTG': 185172, 'CCGCA': 175713, 'CCGCT': 233767, 'CCGCC': 317749, 'CCGCG': 164208, 'CCGGA': 162192, 'CCGGT': 162748, 'CCGGC': 296571, 'CCGGG': 357253, 'CGAAA': 46891, 'CGAAT': 43527, 'CGAAC': 66711, 'CGAAG': 156210, 'CGATA': 22165, 'CGATT': 32215, 'CGATC': 86596, 'CGATG': 188907, 'CGACA': 61011, 'CGACT': 66685, 'CGACC': 78885, 'CGACG': 51881, 'CGAGA': 88550, 'CGAGT': 70350, 'CGAGC': 107038, 'CGAGG': 190617, 'CGTAA': 22446, 'CGTAT': 27315, 'CGTAC': 56892, 'CGTAG': 142628, 'CGTTA': 19479, 'CGTTT': 75966, 'CGTTC': 121918, 'CGTTG': 139433, 'CGTCA': 110343, 'CGTCT': 155351, 'CGTCC': 205604, 'CGTCG': 131304, 'CGTGA': 99447, 'CGTGT': 107650, 'CGTGC': 148054, 'CGTGG': 231336, 'CGCAA': 42176, 'CGCAT': 69208, 'CGCAC': 129787, 'CGCAG': 270373, 'CGCTA': 19726, 'CGCTT': 130533, 'CGCTC': 220412, 'CGCTG': 251366, 'CGCCA': 183273, 'CGCCT': 182045, 'CGCCC': 254293, 'CGCCG': 210983, 'CGCGA': 57334, 'CGCGT': 67607, 'CGCGC': 131702, 'CGCGG': 194504, 'CGGAA': 105870, 'CGGAT': 129004, 'CGGAC': 106607, 'CGGAG': 192313, 'CGGTA': 92669, 'CGGTT': 88285, 'CGGTC': 142715, 'CGGTG': 220680, 'CGGCA': 183405, 'CGGCT': 217295, 'CGGCC': 359800, 'CGGCG': 243172, 'CGGGA': 193759, 'CGGGT': 137246, 'CGGGC': 341805, 'CGGGG': 365928, 'GAAAA': 158255, 'GAAAT': 135550, 'GAAAC': 121811, 'GAAAG': 169823, 'GAATA': 67412, 'GAATT': 96349, 'GAATC': 105474, 'GAATG': 119919, 'GAACA': 160450, 'GAACT': 217199, 'GAACC': 123462, 'GAACG': 54657, 'GAAGA': 276801, 'GAAGT': 227140, 'GAAGC': 292566, 'GAAGG': 394622, 'GATAA': 58906, 'GATAT': 51167, 'GATAC': 55688, 'GATAG': 67946, 'GATTA': 38924, 'GATTT': 104480, 'GATTC': 86015, 'GATTG': 64091, 'GATCA': 148310, 'GATCT': 267022, 'GATCC': 135432, 'GATCG': 37985, 'GATGA': 287939, 'GATGT': 264031, 'GATGC': 218296, 'GATGG': 341226, 'GACAA': 108984, 'GACAT': 127713, 'GACAC': 155298, 'GACAG': 246254, 'GACTA': 35637, 'GACTT': 150112, 'GACTC': 160262, 'GACTG': 142812, 'GACCA': 212623, 'GACCT': 180739, 'GACCC': 183592, 'GACCG': 85968, 'GACGA': 88987, 'GACGT': 101227, 'GACGC': 109883, 'GACGG': 144061, 'GAGAA': 238081, 'GAGAT': 149073, 'GAGAC': 176478, 'GAGAG': 267269, 'GAGTA': 69833, 'GAGTT': 126375, 'GAGTC': 177053, 'GAGTG': 175092, 'GAGCA': 224552, 'GAGCT': 288736, 'GAGCC': 296822, 'GAGCG': 157390, 'GAGGA': 374398, 'GAGGT': 273229, 'GAGGC': 420272, 'GAGGG': 456257, 'GTAAA': 71352, 'GTAAT': 75683, 'GTAAC': 50128, 'GTAAG': 60270, 'GTATA': 40803, 'GTATT': 83481, 'GTATC': 56515, 'GTATG': 58202, 'GTACA': 127186, 'GTACT': 146225, 'GTACC': 74846, 'GTACG': 37207, 'GTAGA': 163760, 'GTAGT': 145556, 'GTAGC': 158123, 'GTAGG': 203703, 'GTTAA': 62154, 'GTTAT': 51199, 'GTTAC': 49308, 'GTTAG': 44892, 'GTTTA': 53562, 'GTTTT': 161689, 'GTTTC': 181722, 'GTTTG': 151910, 'GTTCA': 192162, 'GTTCT': 264673, 'GTTCC': 165458, 'GTTCG': 48181, 'GTTGA': 207963, 'GTTGT': 198257, 'GTTGC': 146564, 'GTTGG': 273576, 'GTCAA': 122130, 'GTCAT': 165127, 'GTCAC': 218057, 'GTCAG': 231268, 'GTCTA': 51980, 'GTCTT': 248783, 'GTCTC': 251852, 'GTCTG': 252890, 'GTCCA': 324288, 'GTCCT': 347202, 'GTCCC': 306293, 'GTCCG': 143416, 'GTCGA': 88965, 'GTCGT': 128880, 'GTCGC': 105047, 'GTCGG': 170033, 'GTGAA': 172906, 'GTGAT': 139786, 'GTGAC': 166626, 'GTGAG': 246536, 'GTGTA': 97795, 'GTGTT': 147867, 'GTGTC': 185216, 'GTGTG': 211687, 'GTGCA': 201985, 'GTGCT': 264994, 'GTGCC': 236693, 'GTGCG': 122336, 'GTGGA': 288679, 'GTGGT': 243671, 'GTGGC': 397186, 'GTGGG': 469800, 'GCAAA': 149684, 'GCAAT': 89055, 'GCAAC': 95902, 'GCAAG': 138014, 'GCATA': 82888, 'GCATT': 98695, 'GCATC': 192216, 'GCATG': 197460, 'GCACA': 236484, 'GCACT': 204987, 'GCACC': 246953, 'GCACG': 170553, 'GCAGA': 274923, 'GCAGT': 199427, 'GCAGC': 563309, 'GCAGG': 660703, 'GCTAA': 55101, 'GCTAT': 34112, 'GCTAC': 58201, 'GCTAG': 62344, 'GCTTA': 59604, 'GCTTT': 180163, 'GCTTC': 356249, 'GCTTG': 313202, 'GCTCA': 273400, 'GCTCT': 273178, 'GCTCC': 476816, 'GCTCG': 207728, 'GCTGA': 249333, 'GCTGT': 316919, 'GCTGC': 551874, 'GCTGG': 657284, 'GCCAA': 169837, 'GCCAT': 221300, 'GCCAC': 371014, 'GCCAG': 496740, 'GCCTA': 48017, 'GCCTT': 256615, 'GCCTC': 432085, 'GCCTG': 413600, 'GCCCA': 410887, 'GCCCT': 340767, 'GCCCC': 436414, 'GCCCG': 286060, 'GCCGA': 137814, 'GCCGT': 191170, 'GCCGC': 373849, 'GCCGG': 335344, 'GCGAA': 59244, 'GCGAT': 82178, 'GCGAC': 73014, 'GCGAG': 137205, 'GCGTA': 49013, 'GCGTT': 88175, 'GCGTC': 159640, 'GCGTG': 192071, 'GCGCA': 173326, 'GCGCT': 195592, 'GCGCC': 251631, 'GCGCG': 152128, 'GCGGA': 187712, 'GCGGT': 186895, 'GCGGC': 383316, 'GCGGG': 341091, 'GGAAA': 194635, 'GGAAT': 131722, 'GGAAC': 180679, 'GGAAG': 420627, 'GGATA': 57960, 'GGATT': 102302, 'GGATC': 204074, 'GGATG': 353934, 'GGACA': 256825, 'GGACT': 197885, 'GGACC': 223347, 'GGACG': 139412, 'GGAGA': 331269, 'GGAGT': 221031, 'GGAGC': 368047, 'GGAGG': 606482, 'GGTAA': 67366, 'GGTAT': 73919, 'GGTAC': 137888, 'GGTAG': 206862, 'GGTTA': 53202, 'GGTTT': 152254, 'GGTTC': 197302, 'GGTTG': 250003, 'GGTCA': 282747, 'GGTCT': 273598, 'GGTCC': 366663, 'GGTCG': 136572, 'GGTGA': 311155, 'GGTGT': 223255, 'GGTGC': 317258, 'GGTGG': 605488, 'GGCAA': 180417, 'GGCAT': 202884, 'GGCAC': 289300, 'GGCAG': 617624, 'GGCTA': 69479, 'GGCTT': 297515, 'GGCTC': 381295, 'GGCTG': 619909, 'GGCCA': 502818, 'GGCCT': 465935, 'GGCCC': 557758, 'GGCCG': 388340, 'GGCGA': 148295, 'GGCGT': 197452, 'GGCGC': 300303, 'GGCGG': 432027, 'GGGAA': 249778, 'GGGAT': 196552, 'GGGAC': 271383, 'GGGAG': 500620, 'GGGTA': 119083, 'GGGTT': 201358, 'GGGTC': 330173, 'GGGTG': 450738, 'GGGCA': 467124, 'GGGCT': 459468, 'GGGCC': 650376, 'GGGCG': 321114, 'GGGGA': 417652, 'GGGGT': 411263, 'GGGGC': 658371, 'GGGGG': 593863}, 'overrepresented_sequences': {}} {'total_reads': 3606807, 'total_bases': 227201770, 'q20_bases': 217547438, 'q30_bases': 208472209, 'total_cycles': 63, 'quality_curves': {'A': [37.1301, 37.1279, 37.1346, 37.149, 37.1333, 37.1653, 37.1134, 37.1023, 37.1213, 37.1012, 37.0908, 37.0555, 37.0202, 36.9325, 36.9576, 36.9077, 36.8656, 36.8316, 36.8419, 36.8633, 36.804, 36.7945, 36.762, 36.6606, 36.551, 36.4247, 36.2997, 36.2506, 36.1061, 36.0293, 36.0169, 35.9026, 35.8368, 35.7855, 35.7451, 35.7404, 35.5895, 35.5175, 35.495, 35.4444, 35.3142, 35.1704, 35.1723, 35.1246, 35.0532, 34.9402, 34.9133, 34.8241, 34.8001, 34.5784, 34.4557, 34.3512, 34.2915, 34.2471, 34.0205, 33.8928, 33.7407, 33.412, 33.3352, 32.8572, 32.4297, 31.4372, 18.5758], 'T': [37.3805, 37.3646, 37.3869, 37.3498, 37.3197, 37.2986, 37.2565, 37.2388, 37.2382, 37.2263, 37.1899, 37.172, 37.1683, 37.0994, 37.1657, 37.1661, 37.1129, 37.0532, 37.0485, 37.0414, 37.0311, 37.0085, 36.9839, 36.9033, 36.8328, 36.7092, 36.5953, 36.5621, 36.4717, 36.4056, 36.398, 36.2991, 36.2257, 36.1807, 36.1464, 36.1233, 36.0264, 35.9346, 35.8762, 35.8247, 35.6993, 35.6003, 35.5876, 35.5367, 35.4507, 35.3558, 35.3428, 35.2742, 35.2192, 35.0738, 34.9107, 34.8571, 34.7071, 34.6941, 34.4836, 34.377, 34.2381, 33.9232, 33.8199, 33.3394, 33.0002, 32.1533, 20.5206], 'C': [37.335, 37.3612, 37.3237, 37.3321, 37.3552, 37.3176, 37.3269, 37.3226, 37.2998, 37.3194, 37.3147, 37.2978, 37.253, 37.2538, 37.222, 37.1983, 37.202, 37.1945, 37.1676, 37.1549, 37.1148, 37.119, 37.0856, 37.0119, 36.9272, 36.8765, 36.751, 36.7098, 36.6462, 36.5533, 36.5537, 36.4985, 36.4158, 36.4142, 36.3854, 36.3427, 36.2849, 36.2105, 36.1741, 36.1304, 36.0594, 35.9792, 35.9449, 35.934, 35.855, 35.7584, 35.7423, 35.7086, 35.6231, 35.5005, 35.3992, 35.2645, 35.1873, 35.1138, 34.984, 34.8516, 34.6621, 34.4228, 34.2368, 33.8932, 33.4315, 32.5953, 24.5715], 'G': [37.1593, 37.1552, 37.1446, 37.1496, 37.1467, 37.1509, 37.1239, 37.136, 37.1305, 37.1312, 37.123, 37.0813, 37.0548, 36.9908, 36.9683, 36.9476, 36.9518, 36.9219, 36.9356, 36.9391, 36.8712, 36.8597, 36.8197, 36.7512, 36.6939, 36.6111, 36.5122, 36.4679, 36.3885, 36.2973, 36.3188, 36.2154, 36.1447, 36.138, 36.1117, 36.1306, 36.0232, 35.945, 35.9036, 35.8872, 35.7661, 35.694, 35.6378, 35.6338, 35.5492, 35.4362, 35.395, 35.3012, 35.2533, 35.139, 34.9755, 34.8797, 34.7343, 34.7248, 34.5773, 34.4264, 34.2114, 33.9666, 33.8079, 33.355, 32.8154, 32.0527, 24.4641], 'mean': [37.2535, 37.2543, 37.2467, 37.2439, 37.2401, 37.2324, 37.2066, 37.2041, 37.1983, 37.1981, 37.1846, 37.156, 37.1276, 37.0766, 37.0805, 37.0573, 37.04, 37.0082, 37.0065, 37.0074, 36.9606, 36.9514, 36.9194, 36.839, 36.7621, 36.6719, 36.5546, 36.5138, 36.425, 36.3389, 36.3426, 36.2528, 36.1764, 36.1547, 36.1259, 36.1096, 36.0115, 35.9344, 35.8906, 35.8538, 35.746, 35.6474, 35.6197, 35.5993, 35.5144, 35.4087, 35.3891, 35.3133, 35.26, 35.1164, 34.977, 34.8788, 34.7717, 34.7324, 34.5608, 34.4332, 34.2503, 33.9776, 33.8417, 33.4041, 32.9544, 32.1103, 22.5085]}, 'content_curves': {'A': [0.202093, 0.195194, 0.202197, 0.202247, 0.198098, 0.203987, 0.201519, 0.197071, 0.204098, 0.198694, 0.200338, 0.204912, 0.203849, 0.198, 0.205393, 0.202246, 0.199163, 0.204492, 0.200905, 0.196576, 0.203034, 0.20117, 0.197049, 0.201933, 0.197031, 0.195756, 0.200782, 0.196095, 0.194262, 0.199496, 0.196838, 0.195423, 0.200674, 0.197859, 0.194284, 0.200815, 0.198308, 0.195472, 0.20288, 0.197649, 0.196278, 0.200366, 0.198165, 0.193179, 0.199197, 0.199563, 0.193654, 0.201005, 0.195353, 0.195903, 0.199135, 0.196228, 0.195688, 0.200103, 0.198442, 0.195544, 0.199769, 0.197381, 0.194419, 0.200058, 0.197049, 0.194369, 0.190308], 'T': [0.245499, 0.22932, 0.235205, 0.228497, 0.223447, 0.230324, 0.227257, 0.225814, 0.230993, 0.23272, 0.223164, 0.228355, 0.230397, 0.220368, 0.224319, 0.225499, 0.219557, 0.224357, 0.227207, 0.222708, 0.224283, 0.22615, 0.221232, 0.224255, 0.22525, 0.221102, 0.225091, 0.227032, 0.222776, 0.225458, 0.226218, 0.221607, 0.226662, 0.228378, 0.224564, 0.226814, 0.226612, 0.222182, 0.221359, 0.22527, 0.220388, 0.225803, 0.22605, 0.223085, 0.225013, 0.227783, 0.222171, 0.225625, 0.227265, 0.223155, 0.221737, 0.226927, 0.218479, 0.221105, 0.223475, 0.216953, 0.221924, 0.219517, 0.216518, 0.219027, 0.222638, 0.215771, 0.208251], 'C': [0.26061, 0.273889, 0.264135, 0.266965, 0.275359, 0.266956, 0.269508, 0.276287, 0.269096, 0.271723, 0.27854, 0.273756, 0.271022, 0.279015, 0.276314, 0.273264, 0.279748, 0.276236, 0.27631, 0.280119, 0.275762, 0.274467, 0.281054, 0.276164, 0.279048, 0.284956, 0.277715, 0.277526, 0.282682, 0.275873, 0.278245, 0.282661, 0.277314, 0.277724, 0.283656, 0.278131, 0.281166, 0.283445, 0.280753, 0.280333, 0.283933, 0.27861, 0.280576, 0.286278, 0.28206, 0.278466, 0.284886, 0.28007, 0.278659, 0.2815, 0.281803, 0.280447, 0.286919, 0.282814, 0.282632, 0.286557, 0.281776, 0.285087, 0.286993, 0.282656, 0.282219, 0.286602, 0.292188], 'G': [0.291797, 0.301597, 0.298459, 0.302291, 0.303095, 0.298733, 0.301716, 0.300828, 0.295792, 0.29685, 0.297951, 0.292977, 0.294732, 0.302617, 0.293974, 0.29899, 0.301533, 0.294915, 0.295579, 0.300597, 0.296921, 0.298213, 0.300665, 0.297648, 0.298672, 0.298186, 0.296413, 0.299347, 0.30028, 0.299173, 0.298699, 0.300309, 0.29535, 0.29604, 0.297496, 0.29424, 0.293914, 0.298901, 0.295008, 0.296749, 0.2994, 0.295221, 0.295188, 0.297447, 0.293728, 0.294188, 0.29929, 0.2933, 0.298722, 0.299442, 0.297326, 0.296398, 0.298915, 0.295978, 0.295451, 0.300946, 0.296531, 0.298014, 0.302071, 0.298259, 0.298094, 0.303259, 0.307242], 'N': [0, 0, 3.88155e-06, 0, 0, 0, 0, 0, 2.13485e-05, 1.24764e-05, 7.48585e-06, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 2.10727e-05, 1.08138e-05, 1.10912e-06, 0, 0, 0, 0, 0, 0, 0, 0, 5.54705e-07, 0, 0, 0, 8.32249e-07, 0, 0, 0, 0, 0.00201182], 'GC': [0.552407, 0.575486, 0.562594, 0.569256, 0.578454, 0.565689, 0.571224, 0.577115, 0.564888, 0.568574, 0.576491, 0.566733, 0.565754, 0.581632, 0.570288, 0.572255, 0.58128, 0.571151, 0.571888, 0.580716, 0.572683, 0.572679, 0.581718, 0.573812, 0.57772, 0.583143, 0.574127, 0.576873, 0.582963, 0.575046, 0.576944, 0.58297, 0.572665, 0.573764, 0.581152, 0.572371, 0.57508, 0.582347, 0.575761, 0.577082, 0.583334, 0.573831, 0.575764, 0.583725, 0.575788, 0.572654, 0.584176, 0.57337, 0.577382, 0.580942, 0.579129, 0.576845, 0.585833, 0.578792, 0.578083, 0.587503, 0.578306, 0.583101, 0.589064, 0.580915, 0.580313, 0.58986, 0.599429]}, 'kmer_count': {'AAAAA': 435810, 'AAAAT': 209465, 'AAAAC': 160260, 'AAAAG': 206623, 'AAATA': 176544, 'AAATT': 140372, 'AAATC': 127523, 'AAATG': 150519, 'AAACA': 193682, 'AAACT': 170786, 'AAACC': 141505, 'AAACG': 55160, 'AAAGA': 184264, 'AAAGT': 161108, 'AAAGC': 182290, 'AAAGG': 203083, 'AATAA': 137889, 'AATAT': 87093, 'AATAC': 76177, 'AATAG': 80355, 'AATTA': 77742, 'AATTT': 144955, 'AATTC': 116484, 'AATTG': 78082, 'AATCA': 104460, 'AATCT': 159115, 'AATCC': 124213, 'AATCG': 46253, 'AATGA': 126668, 'AATGT': 124986, 'AATGC': 123938, 'AATGG': 174682, 'AACAA': 135449, 'AACAT': 129556, 'AACAC': 149246, 'AACAG': 187383, 'AACTA': 48015, 'AACTT': 195725, 'AACTC': 169872, 'AACTG': 183389, 'AACCA': 128401, 'AACCT': 119715, 'AACCC': 136099, 'AACCG': 50382, 'AACGA': 29994, 'AACGT': 40290, 'AACGC': 50819, 'AACGG': 64810, 'AAGAA': 206324, 'AAGAT': 153178, 'AAGAC': 130498, 'AAGAG': 213072, 'AAGTA': 103692, 'AAGTT': 153297, 'AAGTC': 166386, 'AAGTG': 181667, 'AAGCA': 164077, 'AAGCT': 173415, 'AAGCC': 244492, 'AAGCG': 119802, 'AAGGA': 218438, 'AAGGT': 179992, 'AAGGC': 291017, 'AAGGG': 282230, 'ATAAA': 151236, 'ATAAT': 71231, 'ATAAC': 51511, 'ATAAG': 69092, 'ATATA': 69357, 'ATATT': 83913, 'ATATC': 56236, 'ATATG': 57046, 'ATACA': 106541, 'ATACT': 99670, 'ATACC': 58307, 'ATACG': 22647, 'ATAGA': 85209, 'ATAGT': 80065, 'ATAGC': 96398, 'ATAGG': 100682, 'ATTAA': 73082, 'ATTAT': 53320, 'ATTAC': 64408, 'ATTAG': 49985, 'ATTTA': 73714, 'ATTTT': 182142, 'ATTTC': 152509, 'ATTTG': 146339, 'ATTCA': 126874, 'ATTCT': 156869, 'ATTCC': 134886, 'ATTCG': 47618, 'ATTGA': 94309, 'ATTGT': 81866, 'ATTGC': 87924, 'ATTGG': 113961, 'ATCAA': 101363, 'ATCAT': 181681, 'ATCAC': 135377, 'ATCAG': 185013, 'ATCTA': 44295, 'ATCTT': 321967, 'ATCTC': 256201, 'ATCTG': 247693, 'ATCCA': 205376, 'ATCCT': 196255, 'ATCCC': 141965, 'ATCCG': 87213, 'ATCGA': 41274, 'ATCGT': 58748, 'ATCGC': 59816, 'ATCGG': 60981, 'ATGAA': 183448, 'ATGAT': 189527, 'ATGAC': 141204, 'ATGAG': 227526, 'ATGTA': 114483, 'ATGTT': 215653, 'ATGTC': 210324, 'ATGTG': 147743, 'ATGCA': 123081, 'ATGCT': 150190, 'ATGCC': 205450, 'ATGCG': 133414, 'ATGGA': 180092, 'ATGGT': 228942, 'ATGGC': 303386, 'ATGGG': 249970, 'ACAAA': 210696, 'ACAAT': 117107, 'ACAAC': 91057, 'ACAAG': 144309, 'ACATA': 97046, 'ACATT': 145043, 'ACATC': 162376, 'ACATG': 175951, 'ACACA': 223016, 'ACACT': 130249, 'ACACC': 205968, 'ACACG': 123222, 'ACAGA': 217603, 'ACAGT': 156727, 'ACAGC': 315873, 'ACAGG': 323961, 'ACTAA': 46645, 'ACTAT': 43531, 'ACTAC': 39755, 'ACTAG': 45192, 'ACTTA': 46055, 'ACTTT': 162042, 'ACTTC': 232779, 'ACTTG': 251833, 'ACTCA': 157594, 'ACTCT': 148312, 'ACTCC': 221089, 'ACTCG': 144042, 'ACTGA': 147849, 'ACTGT': 164187, 'ACTGC': 234773, 'ACTGG': 276966, 'ACCAA': 105042, 'ACCAT': 157402, 'ACCAC': 278392, 'ACCAG': 341594, 'ACCTA': 37678, 'ACCTT': 201394, 'ACCTC': 261526, 'ACCTG': 260349, 'ACCCA': 206072, 'ACCCT': 172048, 'ACCCC': 199679, 'ACCCG': 116445, 'ACCGA': 70174, 'ACCGT': 74606, 'ACCGC': 99203, 'ACCGG': 89222, 'ACGAA': 68125, 'ACGAT': 74977, 'ACGAC': 64542, 'ACGAG': 90040, 'ACGTA': 43987, 'ACGTT': 99186, 'ACGTC': 118554, 'ACGTG': 110388, 'ACGCA': 87174, 'ACGCT': 93308, 'ACGCC': 146352, 'ACGCG': 69714, 'ACGGA': 99754, 'ACGGT': 109752, 'ACGGC': 152192, 'ACGGG': 151647, 'AGAAA': 233293, 'AGAAT': 119413, 'AGAAC': 175284, 'AGAAG': 330032, 'AGATA': 98695, 'AGATT': 91692, 'AGATC': 144461, 'AGATG': 269740, 'AGACA': 177037, 'AGACT': 117194, 'AGACC': 191097, 'AGACG': 123487, 'AGAGA': 243256, 'AGAGT': 134440, 'AGAGC': 238360, 'AGAGG': 389896, 'AGTAA': 85714, 'AGTAT': 67747, 'AGTAC': 84362, 'AGTAG': 124551, 'AGTTA': 66362, 'AGTTT': 159511, 'AGTTC': 167336, 'AGTTG': 169046, 'AGTCA': 155913, 'AGTCT': 167014, 'AGTCC': 238154, 'AGTCG': 103699, 'AGTGA': 180363, 'AGTGT': 138141, 'AGTGC': 184007, 'AGTGG': 279038, 'AGCAA': 143380, 'AGCAT': 166493, 'AGCAC': 271999, 'AGCAG': 474053, 'AGCTA': 74637, 'AGCTT': 281496, 'AGCTC': 363778, 'AGCTG': 472436, 'AGCCA': 351586, 'AGCCT': 284128, 'AGCCC': 376735, 'AGCCG': 239303, 'AGCGA': 100361, 'AGCGT': 146043, 'AGCGC': 205003, 'AGCGG': 242484, 'AGGAA': 349460, 'AGGAT': 230695, 'AGGAC': 259122, 'AGGAG': 470253, 'AGGTA': 160846, 'AGGTT': 186689, 'AGGTC': 291762, 'AGGTG': 411183, 'AGGCA': 356698, 'AGGCT': 378451, 'AGGCC': 462415, 'AGGCG': 289264, 'AGGGA': 329994, 'AGGGT': 284208, 'AGGGC': 502028, 'AGGGG': 556995, 'TAAAA': 177758, 'TAAAT': 102521, 'TAAAC': 71597, 'TAAAG': 82094, 'TAATA': 57280, 'TAATT': 80159, 'TAATC': 72695, 'TAATG': 77048, 'TAACA': 78191, 'TAACT': 74561, 'TAACC': 48399, 'TAACG': 14045, 'TAAGA': 72955, 'TAAGT': 51856, 'TAAGC': 61208, 'TAAGG': 89350, 'TATAA': 56788, 'TATAT': 57815, 'TATAC': 37179, 'TATAG': 48139, 'TATTA': 42976, 'TATTT': 143144, 'TATTC': 78194, 'TATTG': 77818, 'TATCA': 66678, 'TATCT': 78247, 'TATCC': 67596, 'TATCG': 26824, 'TATGA': 56308, 'TATGT': 57712, 'TATGC': 41881, 'TATGG': 82383, 'TACAA': 95539, 'TACAT': 90965, 'TACAC': 93034, 'TACAG': 141752, 'TACTA': 26997, 'TACTT': 139819, 'TACTC': 103887, 'TACTG': 111890, 'TACCA': 77621, 'TACCT': 65098, 'TACCC': 71129, 'TACCG': 35635, 'TACGA': 22840, 'TACGT': 31078, 'TACGC': 23677, 'TACGG': 48834, 'TAGAA': 112277, 'TAGAT': 87516, 'TAGAC': 68336, 'TAGAG': 119560, 'TAGTA': 69447, 'TAGTT': 80612, 'TAGTC': 90299, 'TAGTG': 97888, 'TAGCA': 86356, 'TAGCT': 99718, 'TAGCC': 131675, 'TAGCG': 76229, 'TAGGA': 126352, 'TAGGT': 110578, 'TAGGC': 127341, 'TAGGG': 150199, 'TTAAA': 139059, 'TTAAT': 91151, 'TTAAC': 59323, 'TTAAG': 77706, 'TTATA': 56662, 'TTATT': 100487, 'TTATC': 66736, 'TTATG': 59438, 'TTACA': 102219, 'TTACT': 57898, 'TTACC': 48496, 'TTACG': 30427, 'TTAGA': 47108, 'TTAGT': 63315, 'TTAGC': 60166, 'TTAGG': 85521, 'TTTAA': 138368, 'TTTAT': 116643, 'TTTAC': 76255, 'TTTAG': 75011, 'TTTTA': 141608, 'TTTTT': 618699, 'TTTTC': 253836, 'TTTTG': 200837, 'TTTCA': 189214, 'TTTCT': 361217, 'TTTCC': 237856, 'TTTCG': 46167, 'TTTGA': 158175, 'TTTGT': 221939, 'TTTGC': 178142, 'TTTGG': 217541, 'TTCAA': 169056, 'TTCAT': 229106, 'TTCAC': 267652, 'TTCAG': 322105, 'TTCTA': 83411, 'TTCTT': 533813, 'TTCTC': 475221, 'TTCTG': 362852, 'TTCCA': 252455, 'TTCCT': 327700, 'TTCCC': 261566, 'TTCCG': 160598, 'TTCGA': 56986, 'TTCGT': 47524, 'TTCGC': 50690, 'TTCGG': 81427, 'TTGAA': 211328, 'TTGAT': 224538, 'TTGAC': 170173, 'TTGAG': 254979, 'TTGTA': 175311, 'TTGTT': 235721, 'TTGTC': 266260, 'TTGTG': 191680, 'TTGCA': 167657, 'TTGCT': 223517, 'TTGCC': 236191, 'TTGCG': 151877, 'TTGGA': 240872, 'TTGGT': 267090, 'TTGGC': 339815, 'TTGGG': 328480, 'TCAAA': 201646, 'TCAAT': 157039, 'TCAAC': 90680, 'TCAAG': 135780, 'TCATA': 117671, 'TCATT': 138216, 'TCATC': 356376, 'TCATG': 244607, 'TCACA': 242426, 'TCACT': 215270, 'TCACC': 263359, 'TCACG': 130272, 'TCAGA': 193219, 'TCAGT': 180896, 'TCAGC': 387904, 'TCAGG': 391890, 'TCTAA': 56133, 'TCTAT': 63751, 'TCTAC': 76662, 'TCTAG': 77279, 'TCTTA': 90165, 'TCTTT': 288427, 'TCTTC': 571263, 'TCTTG': 528080, 'TCTCA': 212278, 'TCTCT': 324955, 'TCTCC': 540121, 'TCTCG': 235015, 'TCTGA': 208176, 'TCTGT': 246389, 'TCTGC': 376243, 'TCTGG': 497562, 'TCCAA': 170321, 'TCCAT': 232581, 'TCCAC': 415575, 'TCCAG': 634393, 'TCCTA': 71514, 'TCCTT': 410713, 'TCCTC': 700561, 'TCCTG': 436134, 'TCCCA': 314427, 'TCCCT': 221840, 'TCCCC': 329837, 'TCCCG': 268964, 'TCCGA': 102231, 'TCCGT': 143596, 'TCCGC': 200458, 'TCCGG': 241770, 'TCGAA': 120761, 'TCGAT': 114697, 'TCGAC': 48730, 'TCGAG': 73078, 'TCGTA': 96558, 'TCGTT': 87275, 'TCGTC': 180484, 'TCGTG': 121013, 'TCGCA': 97038, 'TCGCT': 125429, 'TCGCC': 151542, 'TCGCG': 85531, 'TCGGA': 106703, 'TCGGT': 108543, 'TCGGC': 216752, 'TCGGG': 232618, 'TGAAA': 146442, 'TGAAT': 118489, 'TGAAC': 169188, 'TGAAG': 354227, 'TGATA': 71895, 'TGATT': 83205, 'TGATC': 185846, 'TGATG': 359142, 'TGACA': 178515, 'TGACT': 133802, 'TGACC': 214000, 'TGACG': 159302, 'TGAGA': 216761, 'TGAGT': 151318, 'TGAGC': 316648, 'TGAGG': 422816, 'TGTAA': 98916, 'TGTAT': 83688, 'TGTAC': 127446, 'TGTAG': 233422, 'TGTTA': 80563, 'TGTTT': 195203, 'TGTTC': 226509, 'TGTTG': 314667, 'TGTCA': 220209, 'TGTCT': 252470, 'TGTCC': 369592, 'TGTCG': 149291, 'TGTGA': 164674, 'TGTGT': 204430, 'TGTGC': 214689, 'TGTGG': 344826, 'TGCAA': 135012, 'TGCAT': 165631, 'TGCAC': 224707, 'TGCAG': 438051, 'TGCTA': 62085, 'TGCTT': 259088, 'TGCTC': 340874, 'TGCTG': 536236, 'TGCCA': 294481, 'TGCCT': 276148, 'TGCCC': 364850, 'TGCCG': 257802, 'TGCGA': 62918, 'TGCGT': 104010, 'TGCGC': 178817, 'TGCGG': 286969, 'TGGAA': 282132, 'TGGAT': 203739, 'TGGAC': 232099, 'TGGAG': 468399, 'TGGTA': 145825, 'TGGTT': 211128, 'TGGTC': 367458, 'TGGTG': 453509, 'TGGCA': 357463, 'TGGCT': 391625, 'TGGCC': 557007, 'TGGCG': 288480, 'TGGGA': 344238, 'TGGGT': 333586, 'TGGGC': 504223, 'TGGGG': 709046, 'CAAAA': 218359, 'CAAAT': 130248, 'CAAAC': 194127, 'CAAAG': 253930, 'CAATA': 71813, 'CAATT': 88616, 'CAATC': 113985, 'CAATG': 186450, 'CAACA': 149958, 'CAACT': 110279, 'CAACC': 109203, 'CAACG': 55734, 'CAAGA': 139057, 'CAAGT': 137889, 'CAAGC': 135288, 'CAAGG': 235669, 'CATAA': 82348, 'CATAT': 64332, 'CATAC': 111631, 'CATAG': 159398, 'CATTA': 74712, 'CATTT': 149147, 'CATTC': 175480, 'CATTG': 149867, 'CATCA': 267694, 'CATCT': 335983, 'CATCC': 288823, 'CATCG': 104877, 'CATGA': 239450, 'CATGT': 213697, 'CATGC': 201726, 'CATGG': 321237, 'CACAA': 210205, 'CACAT': 217186, 'CACAC': 266700, 'CACAG': 409534, 'CACTA': 60348, 'CACTT': 190307, 'CACTC': 217638, 'CACTG': 367476, 'CACCA': 439962, 'CACCT': 375188, 'CACCC': 281710, 'CACCG': 151663, 'CACGA': 141186, 'CACGT': 188385, 'CACGC': 199142, 'CACGG': 240053, 'CAGAA': 272416, 'CAGAT': 196608, 'CAGAC': 212040, 'CAGAG': 374717, 'CAGTA': 112819, 'CAGTT': 186513, 'CAGTC': 209775, 'CAGTG': 307060, 'CAGCA': 551176, 'CAGCT': 598128, 'CAGCC': 548478, 'CAGCG': 323079, 'CAGGA': 543717, 'CAGGT': 449646, 'CAGGC': 600566, 'CAGGG': 723933, 'CTAAA': 63345, 'CTAAT': 40602, 'CTAAC': 48486, 'CTAAG': 60823, 'CTATA': 29064, 'CTATT': 63972, 'CTATC': 53017, 'CTATG': 54395, 'CTACA': 72890, 'CTACT': 63143, 'CTACC': 61231, 'CTACG': 31445, 'CTAGA': 74582, 'CTAGT': 30975, 'CTAGC': 59964, 'CTAGG': 96855, 'CTTAA': 85117, 'CTTAT': 55581, 'CTTAC': 43909, 'CTTAG': 80028, 'CTTTA': 128267, 'CTTTT': 225785, 'CTTTC': 225898, 'CTTTG': 257356, 'CTTCA': 457061, 'CTTCT': 637940, 'CTTCC': 443944, 'CTTCG': 89247, 'CTTGA': 374694, 'CTTGT': 343319, 'CTTGC': 349807, 'CTTGG': 540149, 'CTCAA': 177615, 'CTCAT': 260961, 'CTCAC': 204816, 'CTCAG': 383960, 'CTCTA': 86808, 'CTCTT': 341526, 'CTCTC': 296800, 'CTCTG': 430097, 'CTCCA': 630001, 'CTCCT': 706500, 'CTCCC': 390238, 'CTCCG': 282507, 'CTCGA': 158689, 'CTCGT': 235348, 'CTCGC': 233254, 'CTCGG': 331256, 'CTGAA': 199831, 'CTGAT': 129147, 'CTGAC': 190180, 'CTGAG': 349026, 'CTGTA': 144330, 'CTGTT': 200830, 'CTGTC': 307162, 'CTGTG': 349963, 'CTGCA': 446904, 'CTGCT': 529953, 'CTGCC': 489297, 'CTGCG': 209466, 'CTGGA': 437699, 'CTGGT': 405466, 'CTGGC': 509477, 'CTGGG': 788110, 'CCAAA': 219605, 'CCAAT': 87849, 'CCAAC': 138224, 'CCAAG': 212075, 'CCATA': 111012, 'CCATT': 154829, 'CCATC': 264211, 'CCATG': 337503, 'CCACA': 374837, 'CCACT': 262707, 'CCACC': 508125, 'CCACG': 324150, 'CCAGA': 337782, 'CCAGT': 255334, 'CCAGC': 691959, 'CCAGG': 865976, 'CCTAA': 48890, 'CCTAT': 54756, 'CCTAC': 47943, 'CCTAG': 69810, 'CCTTA': 61323, 'CCTTT': 187899, 'CCTTC': 424959, 'CCTTG': 473926, 'CCTCA': 354354, 'CCTCT': 378290, 'CCTCC': 719306, 'CCTCG': 347585, 'CCTGA': 232008, 'CCTGT': 239474, 'CCTGC': 447587, 'CCTGG': 632731, 'CCCAA': 191991, 'CCCAT': 228793, 'CCCAC': 362779, 'CCCAG': 615097, 'CCCTA': 58835, 'CCCTT': 247024, 'CCCTC': 358114, 'CCCTG': 390712, 'CCCCA': 420696, 'CCCCT': 281264, 'CCCCC': 355836, 'CCCCG': 308763, 'CCCGA': 157124, 'CCCGT': 166961, 'CCCGC': 286270, 'CCCGG': 404041, 'CCGAA': 97313, 'CCGAT': 86432, 'CCGAC': 98279, 'CCGAG': 202079, 'CCGTA': 81470, 'CCGTT': 111579, 'CCGTC': 198833, 'CCGTG': 207818, 'CCGCA': 197314, 'CCGCT': 263619, 'CCGCC': 361498, 'CCGCG': 185899, 'CCGGA': 183605, 'CCGGT': 182313, 'CCGGC': 339195, 'CCGGG': 406711, 'CGAAA': 52310, 'CGAAT': 50105, 'CGAAC': 73800, 'CGAAG': 174677, 'CGATA': 24418, 'CGATT': 35288, 'CGATC': 97371, 'CGATG': 211970, 'CGACA': 68722, 'CGACT': 75569, 'CGACC': 89736, 'CGACG': 60527, 'CGAGA': 100789, 'CGAGT': 78767, 'CGAGC': 118865, 'CGAGG': 217136, 'CGTAA': 24340, 'CGTAT': 30510, 'CGTAC': 62775, 'CGTAG': 159803, 'CGTTA': 21704, 'CGTTT': 85094, 'CGTTC': 134916, 'CGTTG': 156461, 'CGTCA': 122670, 'CGTCT': 173528, 'CGTCC': 230726, 'CGTCG': 146838, 'CGTGA': 109718, 'CGTGT': 119368, 'CGTGC': 165762, 'CGTGG': 258278, 'CGCAA': 47407, 'CGCAT': 76545, 'CGCAC': 143979, 'CGCAG': 305530, 'CGCTA': 22199, 'CGCTT': 145565, 'CGCTC': 250937, 'CGCTG': 283415, 'CGCCA': 205570, 'CGCCT': 202956, 'CGCCC': 287988, 'CGCCG': 242722, 'CGCGA': 64386, 'CGCGT': 76778, 'CGCGC': 149007, 'CGCGG': 220143, 'CGGAA': 119291, 'CGGAT': 142835, 'CGGAC': 120515, 'CGGAG': 216998, 'CGGTA': 105131, 'CGGTT': 97084, 'CGGTC': 162198, 'CGGTG': 246712, 'CGGCA': 205929, 'CGGCT': 243901, 'CGGCC': 410850, 'CGGCG': 276460, 'CGGGA': 217784, 'CGGGT': 154375, 'CGGGC': 384829, 'CGGGG': 418548, 'GAAAA': 179033, 'GAAAT': 148746, 'GAAAC': 133633, 'GAAAG': 188406, 'GAATA': 74461, 'GAATT': 107579, 'GAATC': 117786, 'GAATG': 132246, 'GAACA': 178917, 'GAACT': 240682, 'GAACC': 137378, 'GAACG': 61010, 'GAAGA': 307180, 'GAAGT': 254259, 'GAAGC': 322477, 'GAAGG': 445536, 'GATAA': 66044, 'GATAT': 57688, 'GATAC': 62352, 'GATAG': 75140, 'GATTA': 43572, 'GATTT': 116227, 'GATTC': 95159, 'GATTG': 71375, 'GATCA': 162932, 'GATCT': 293919, 'GATCC': 152061, 'GATCG': 43927, 'GATGA': 317136, 'GATGT': 290403, 'GATGC': 243703, 'GATGG': 384612, 'GACAA': 121165, 'GACAT': 141434, 'GACAC': 173464, 'GACAG': 275727, 'GACTA': 39071, 'GACTT': 167379, 'GACTC': 180374, 'GACTG': 160939, 'GACCA': 237042, 'GACCT': 200829, 'GACCC': 210250, 'GACCG': 95901, 'GACGA': 103695, 'GACGT': 112167, 'GACGC': 124228, 'GACGG': 160194, 'GAGAA': 266446, 'GAGAT': 168205, 'GAGAC': 200149, 'GAGAG': 298813, 'GAGTA': 76558, 'GAGTT': 141560, 'GAGTC': 198414, 'GAGTG': 194866, 'GAGCA': 254407, 'GAGCT': 322855, 'GAGCC': 331712, 'GAGCG': 176015, 'GAGGA': 416235, 'GAGGT': 305562, 'GAGGC': 472115, 'GAGGG': 519565, 'GTAAA': 78903, 'GTAAT': 82646, 'GTAAC': 54680, 'GTAAG': 67360, 'GTATA': 44458, 'GTATT': 90882, 'GTATC': 61412, 'GTATG': 67203, 'GTACA': 139373, 'GTACT': 163285, 'GTACC': 82900, 'GTACG': 42274, 'GTAGA': 180393, 'GTAGT': 163272, 'GTAGC': 176590, 'GTAGG': 230569, 'GTTAA': 69019, 'GTTAT': 55949, 'GTTAC': 53871, 'GTTAG': 49321, 'GTTTA': 59445, 'GTTTT': 182697, 'GTTTC': 199892, 'GTTTG': 168665, 'GTTCA': 213226, 'GTTCT': 294314, 'GTTCC': 187195, 'GTTCG': 54052, 'GTTGA': 230259, 'GTTGT': 219630, 'GTTGC': 163625, 'GTTGG': 304312, 'GTCAA': 135549, 'GTCAT': 182421, 'GTCAC': 241982, 'GTCAG': 259108, 'GTCTA': 58396, 'GTCTT': 275252, 'GTCTC': 280497, 'GTCTG': 283958, 'GTCCA': 360290, 'GTCCT': 384208, 'GTCCC': 345397, 'GTCCG': 160874, 'GTCGA': 100998, 'GTCGT': 144318, 'GTCGC': 116816, 'GTCGG': 190728, 'GTGAA': 191371, 'GTGAT': 153317, 'GTGAC': 182781, 'GTGAG': 273066, 'GTGTA': 108121, 'GTGTT': 163571, 'GTGTC': 205883, 'GTGTG': 234055, 'GTGCA': 224172, 'GTGCT': 293645, 'GTGCC': 263664, 'GTGCG': 137012, 'GTGGA': 322402, 'GTGGT': 272234, 'GTGGC': 440495, 'GTGGG': 524417, 'GCAAA': 165652, 'GCAAT': 98481, 'GCAAC': 106236, 'GCAAG': 155741, 'GCATA': 92439, 'GCATT': 110026, 'GCATC': 214791, 'GCATG': 219470, 'GCACA': 264383, 'GCACT': 226644, 'GCACC': 276108, 'GCACG': 193519, 'GCAGA': 307669, 'GCAGT': 223728, 'GCAGC': 634880, 'GCAGG': 740190, 'GCTAA': 61138, 'GCTAT': 38083, 'GCTAC': 66680, 'GCTAG': 69944, 'GCTTA': 67145, 'GCTTT': 199122, 'GCTTC': 399160, 'GCTTG': 349847, 'GCTCA': 304926, 'GCTCT': 305802, 'GCTCC': 537755, 'GCTCG': 234592, 'GCTGA': 280334, 'GCTGT': 351254, 'GCTGC': 619346, 'GCTGG': 738020, 'GCCAA': 191088, 'GCCAT': 248660, 'GCCAC': 412743, 'GCCAG': 558171, 'GCCTA': 53547, 'GCCTT': 286657, 'GCCTC': 479004, 'GCCTG': 462834, 'GCCCA': 459205, 'GCCCT': 380091, 'GCCCC': 492633, 'GCCCG': 322905, 'GCCGA': 155533, 'GCCGT': 214730, 'GCCGC': 425155, 'GCCGG': 380351, 'GCGAA': 66107, 'GCGAT': 92034, 'GCGAC': 83251, 'GCGAG': 152429, 'GCGTA': 55437, 'GCGTT': 99465, 'GCGTC': 177052, 'GCGTG': 213716, 'GCGCA': 193092, 'GCGCT': 219501, 'GCGCC': 284922, 'GCGCG': 170687, 'GCGGA': 209426, 'GCGGT': 209480, 'GCGGC': 432648, 'GCGGG': 388711, 'GGAAA': 217583, 'GGAAT': 144042, 'GGAAC': 199772, 'GGAAG': 470779, 'GGATA': 65408, 'GGATT': 114367, 'GGATC': 227007, 'GGATG': 392676, 'GGACA': 286724, 'GGACT': 221618, 'GGACC': 251921, 'GGACG': 156926, 'GGAGA': 373885, 'GGAGT': 246622, 'GGAGC': 412643, 'GGAGG': 685394, 'GGTAA': 74372, 'GGTAT': 82057, 'GGTAC': 154581, 'GGTAG': 233613, 'GGTTA': 58602, 'GGTTT': 170582, 'GGTTC': 219337, 'GGTTG': 277542, 'GGTCA': 319017, 'GGTCT': 305885, 'GGTCC': 412766, 'GGTCG': 153259, 'GGTGA': 344126, 'GGTGT': 248170, 'GGTGC': 352613, 'GGTGG': 678043, 'GGCAA': 200656, 'GGCAT': 227584, 'GGCAC': 322366, 'GGCAG': 690866, 'GGCTA': 77841, 'GGCTT': 330211, 'GGCTC': 429245, 'GGCTG': 695632, 'GGCCA': 563998, 'GGCCT': 519736, 'GGCCC': 630934, 'GGCCG': 440598, 'GGCGA': 165521, 'GGCGT': 219589, 'GGCGC': 336921, 'GGCGG': 494663, 'GGGAA': 278948, 'GGGAT': 218299, 'GGGAC': 306622, 'GGGAG': 563714, 'GGGTA': 132315, 'GGGTT': 229190, 'GGGTC': 373307, 'GGGTG': 504640, 'GGGCA': 520695, 'GGGCT': 516621, 'GGGCC': 732881, 'GGGCG': 365998, 'GGGGA': 470244, 'GGGGT': 463196, 'GGGGC': 745559, 'GGGGG': 701868}, 'overrepresented_sequences': {}} {'total_reads': 3526970, 'total_bases': 204348701, 'q20_bases': 194780404, 'q30_bases': 185629032, 'total_cycles': 58, 'quality_curves': {'A': [36.3771, 36.3578, 36.3917, 36.3933, 36.403, 36.3789, 36.3862, 36.394, 36.3699, 36.3551, 36.372, 36.3275, 36.327, 36.2996, 36.2972, 36.2849, 36.2687, 36.225, 36.1952, 36.1744, 36.1727, 36.1292, 36.098, 36.0774, 36.0883, 36.055, 36.0217, 35.9965, 35.9398, 35.9235, 35.8744, 35.8051, 35.7427, 35.696, 35.726, 35.6744, 35.6279, 35.5644, 35.5266, 35.4794, 35.3856, 35.3381, 35.2702, 35.2159, 35.1022, 34.9703, 34.9314, 34.901, 34.833, 34.7252, 34.5636, 34.3492, 34.2762, 34.0599, 33.9386, 33.8333, 33.5913, 33.4477], 'T': [36.7389, 36.7769, 36.7677, 36.7414, 36.7313, 36.7287, 36.6823, 36.6824, 36.6529, 36.628, 36.6117, 36.5704, 36.5571, 36.5334, 36.5258, 36.5132, 36.48, 36.4339, 36.4449, 36.3769, 36.3557, 36.3177, 36.2825, 36.2358, 36.2237, 36.229, 36.1854, 36.1368, 36.052, 36.0715, 36.0345, 35.9713, 35.8981, 35.8468, 35.8488, 35.8167, 35.7281, 35.683, 35.6251, 35.5878, 35.4593, 35.4367, 35.3564, 35.3354, 35.2131, 35.0959, 35.0243, 34.9856, 34.8571, 34.8018, 34.6653, 34.4698, 34.3577, 34.2295, 34.0009, 33.9967, 33.693, 33.5814], 'C': [36.6907, 36.707, 36.7223, 36.7225, 36.7171, 36.7297, 36.7191, 36.7294, 36.7267, 36.6914, 36.6904, 36.6882, 36.6845, 36.6769, 36.6747, 36.6712, 36.6519, 36.6438, 36.6213, 36.6042, 36.6118, 36.5779, 36.5362, 36.5467, 36.5449, 36.5279, 36.5174, 36.4744, 36.4416, 36.4358, 36.416, 36.3747, 36.3463, 36.3101, 36.3022, 36.2904, 36.2424, 36.1747, 36.1575, 36.103, 36.0325, 35.9943, 35.9354, 35.8501, 35.7976, 35.7033, 35.6059, 35.5823, 35.4524, 35.3759, 35.2915, 35.077, 34.9838, 34.8632, 34.6314, 34.5459, 34.3378, 34.1082], 'G': [36.3112, 36.3324, 36.3576, 36.3421, 36.3383, 36.3422, 36.3382, 36.3139, 36.3239, 36.2928, 36.2915, 36.2704, 36.2734, 36.2426, 36.2362, 36.2226, 36.1985, 36.1813, 36.161, 36.1075, 36.1323, 36.0932, 36.0517, 36.0315, 36.0326, 36.0222, 36.0081, 35.962, 35.9013, 35.9006, 35.861, 35.8096, 35.7897, 35.726, 35.7236, 35.7165, 35.6461, 35.5843, 35.539, 35.4969, 35.3845, 35.3656, 35.2878, 35.227, 35.1338, 35.0278, 34.9365, 34.8965, 34.8243, 34.7226, 34.5672, 34.3698, 34.244, 34.0863, 33.912, 33.7669, 33.5723, 33.3999], 'mean': [36.5265, 36.538, 36.5548, 36.5415, 36.5407, 36.5392, 36.5262, 36.5273, 36.5182, 36.4891, 36.4886, 36.4635, 36.4612, 36.4407, 36.4369, 36.4262, 36.403, 36.3764, 36.3604, 36.3198, 36.3259, 36.2868, 36.2506, 36.2364, 36.2345, 36.2204, 36.1988, 36.1558, 36.0985, 36.1007, 36.0613, 36.0066, 35.9678, 35.9083, 35.9192, 35.8989, 35.8374, 35.7748, 35.7389, 35.6907, 35.5915, 35.5636, 35.4898, 35.43, 35.3385, 35.2306, 35.1516, 35.121, 35.02, 34.9315, 34.8048, 34.5934, 34.4933, 34.3455, 34.1491, 34.059, 33.8321, 33.6573]}, 'content_curves': {'A': [0.212834, 0.217894, 0.213698, 0.218698, 0.225875, 0.215133, 0.219446, 0.221389, 0.217345, 0.220184, 0.224452, 0.218963, 0.219233, 0.222465, 0.213927, 0.218046, 0.222534, 0.217266, 0.219207, 0.221851, 0.217752, 0.218922, 0.222284, 0.215214, 0.22086, 0.221271, 0.214839, 0.219534, 0.222615, 0.217281, 0.221335, 0.223282, 0.216161, 0.219922, 0.223431, 0.215238, 0.217449, 0.223724, 0.218929, 0.220841, 0.225715, 0.215518, 0.22031, 0.221612, 0.217825, 0.218539, 0.224436, 0.217348, 0.220494, 0.222562, 0.215184, 0.217591, 0.220852, 0.214739, 0.22, 0.223662, 0.215378, 0.220142], 'T': [0.207885, 0.200806, 0.200274, 0.193053, 0.196332, 0.193558, 0.19178, 0.200573, 0.196849, 0.194993, 0.201359, 0.196339, 0.195177, 0.199951, 0.197778, 0.196427, 0.198026, 0.200294, 0.195875, 0.199539, 0.19359, 0.191639, 0.197998, 0.19242, 0.19496, 0.198788, 0.196478, 0.196205, 0.200042, 0.19371, 0.192944, 0.198427, 0.195429, 0.195017, 0.197738, 0.197088, 0.192485, 0.196607, 0.194728, 0.191829, 0.194625, 0.194906, 0.194213, 0.200282, 0.19368, 0.193696, 0.196946, 0.193704, 0.192262, 0.19895, 0.19495, 0.191872, 0.197984, 0.195848, 0.193614, 0.197132, 0.194969, 0.193792], 'C': [0.296142, 0.295964, 0.295607, 0.292138, 0.292113, 0.294917, 0.293435, 0.29313, 0.296667, 0.294127, 0.290829, 0.295477, 0.294679, 0.29338, 0.297285, 0.296412, 0.29397, 0.296829, 0.296069, 0.29493, 0.295206, 0.29575, 0.294937, 0.302244, 0.297269, 0.296513, 0.300264, 0.296469, 0.293373, 0.303184, 0.297208, 0.293523, 0.300241, 0.296049, 0.294408, 0.300583, 0.301019, 0.297307, 0.300852, 0.298656, 0.296617, 0.302266, 0.29795, 0.29799, 0.300434, 0.299549, 0.297242, 0.300803, 0.298536, 0.294744, 0.30272, 0.299117, 0.297272, 0.304802, 0.298576, 0.297792, 0.303366, 0.298825], 'G': [0.283139, 0.285335, 0.290421, 0.296112, 0.28568, 0.296392, 0.295328, 0.284909, 0.289139, 0.290696, 0.283318, 0.28917, 0.290897, 0.284199, 0.29101, 0.289115, 0.285466, 0.285544, 0.288849, 0.283599, 0.293452, 0.293669, 0.28478, 0.290122, 0.28691, 0.283424, 0.288419, 0.287791, 0.28397, 0.285818, 0.288481, 0.284767, 0.288169, 0.288787, 0.284423, 0.287069, 0.289047, 0.282361, 0.285484, 0.288652, 0.283043, 0.28731, 0.287514, 0.280057, 0.287963, 0.28821, 0.281375, 0.288145, 0.288707, 0.283744, 0.287146, 0.29134, 0.283885, 0.284611, 0.287786, 0.281414, 0.286286, 0.287241], 'N': [0, 1.41765e-06, 0, 0, 0, 0, 1.10577e-05, 0, 0, 0, 4.30965e-05, 5.10353e-05, 1.36094e-05, 5.67059e-06, 0, 0, 2.83529e-06, 6.66294e-05, 0, 8.1373e-05, 0, 2.01306e-05, 2.83529e-07, 0, 0, 2.8353e-06, 0, 2.83531e-07, 0, 6.80481e-06, 3.17559e-05, 0, 0, 0.000225447, 0, 2.21217e-05, 0, 0, 7.09171e-06, 2.1844e-05, 0, 0, 1.1918e-05, 5.93127e-05, 9.70701e-05, 6.52908e-06, 0, 0, 0, 0, 0, 8.02119e-05, 7.68517e-06, 0, 2.42553e-05, 0, 0, 0], 'GC': [0.579281, 0.581299, 0.586028, 0.58825, 0.577793, 0.591308, 0.588763, 0.578039, 0.585806, 0.584823, 0.574146, 0.584647, 0.585576, 0.577579, 0.588295, 0.585527, 0.579436, 0.582373, 0.584918, 0.578528, 0.588658, 0.589419, 0.579718, 0.592366, 0.584179, 0.579938, 0.588683, 0.58426, 0.577343, 0.589002, 0.585689, 0.578291, 0.58841, 0.584836, 0.578831, 0.587652, 0.590066, 0.579668, 0.586336, 0.587308, 0.57966, 0.589576, 0.585465, 0.578047, 0.588397, 0.587759, 0.578617, 0.588948, 0.587243, 0.578488, 0.589866, 0.590456, 0.581157, 0.589413, 0.586362, 0.579206, 0.589653, 0.586066]}, 'kmer_count': {'AAAAA': 324614, 'AAAAT': 139561, 'AAAAC': 165244, 'AAAAG': 191323, 'AAATA': 107895, 'AAATT': 110769, 'AAATC': 94678, 'AAATG': 129750, 'AAACA': 180722, 'AAACT': 130317, 'AAACC': 153357, 'AAACG': 73174, 'AAAGA': 258123, 'AAAGT': 141924, 'AAAGC': 176530, 'AAAGG': 169010, 'AATAA': 69842, 'AATAT': 67995, 'AATAC': 70665, 'AATAG': 45672, 'AATTA': 61142, 'AATTT': 113808, 'AATTC': 85390, 'AATTG': 68935, 'AATCA': 71041, 'AATCT': 83679, 'AATCC': 105047, 'AATCG': 27913, 'AATGA': 111734, 'AATGT': 120377, 'AATGC': 98571, 'AATGG': 139928, 'AACAA': 216798, 'AACAT': 179963, 'AACAC': 127797, 'AACAG': 187005, 'AACTA': 65311, 'AACTT': 136874, 'AACTC': 123541, 'AACTG': 164196, 'AACCA': 182686, 'AACCT': 168952, 'AACCC': 195360, 'AACCG': 82337, 'AACGA': 75915, 'AACGT': 81110, 'AACGC': 87232, 'AACGG': 97957, 'AAGAA': 472192, 'AAGAT': 268495, 'AAGAC': 242729, 'AAGAG': 305886, 'AAGTA': 115269, 'AAGTT': 165111, 'AAGTC': 159676, 'AAGTG': 165215, 'AAGCA': 225752, 'AAGCT': 256512, 'AAGCC': 282443, 'AAGCG': 143120, 'AAGGA': 355211, 'AAGGT': 197949, 'AAGGC': 266980, 'AAGGG': 221948, 'ATAAA': 87007, 'ATAAT': 42626, 'ATAAC': 46605, 'ATAAG': 46496, 'ATATA': 45951, 'ATATT': 66339, 'ATATC': 54990, 'ATATG': 54127, 'ATACA': 73113, 'ATACT': 56036, 'ATACC': 59425, 'ATACG': 25809, 'ATAGA': 48608, 'ATAGT': 37784, 'ATAGC': 37111, 'ATAGG': 41509, 'ATTAA': 69195, 'ATTAT': 53327, 'ATTAC': 70498, 'ATTAG': 29542, 'ATTTA': 73477, 'ATTTT': 166247, 'ATTTC': 124098, 'ATTTG': 103651, 'ATTCA': 105492, 'ATTCT': 108006, 'ATTCC': 108550, 'ATTCG': 48763, 'ATTGA': 129760, 'ATTGT': 95451, 'ATTGC': 87393, 'ATTGG': 74683, 'ATCAA': 190383, 'ATCAT': 166256, 'ATCAC': 129859, 'ATCAG': 117555, 'ATCTA': 73675, 'ATCTT': 154635, 'ATCTC': 151981, 'ATCTG': 170240, 'ATCCA': 183933, 'ATCCT': 206260, 'ATCCC': 181377, 'ATCCG': 136755, 'ATCGA': 107550, 'ATCGT': 68510, 'ATCGC': 75758, 'ATCGG': 71187, 'ATGAA': 206120, 'ATGAT': 165528, 'ATGAC': 155465, 'ATGAG': 230398, 'ATGTA': 72891, 'ATGTT': 107862, 'ATGTC': 141378, 'ATGTG': 191894, 'ATGCA': 142731, 'ATGCT': 145968, 'ATGCC': 211489, 'ATGCG': 68709, 'ATGGA': 220297, 'ATGGT': 155961, 'ATGGC': 245911, 'ATGGG': 201473, 'ACAAA': 186760, 'ACAAT': 75969, 'ACAAC': 181884, 'ACAAG': 328202, 'ACATA': 46519, 'ACATT': 109727, 'ACATC': 245090, 'ACATG': 192872, 'ACACA': 173296, 'ACACT': 108804, 'ACACC': 198085, 'ACACG': 100526, 'ACAGA': 223660, 'ACAGT': 140791, 'ACAGC': 301205, 'ACAGG': 211663, 'ACTAA': 50243, 'ACTAT': 64756, 'ACTAC': 137628, 'ACTAG': 26129, 'ACTTA': 47341, 'ACTTT': 137409, 'ACTTC': 217385, 'ACTTG': 103260, 'ACTCA': 124905, 'ACTCT': 123967, 'ACTCC': 210271, 'ACTCG': 69826, 'ACTGA': 155491, 'ACTGT': 141134, 'ACTGC': 206417, 'ACTGG': 233843, 'ACCAA': 236389, 'ACCAT': 220172, 'ACCAC': 219979, 'ACCAG': 360863, 'ACCTA': 94984, 'ACCTT': 156535, 'ACCTC': 257919, 'ACCTG': 397617, 'ACCCA': 271304, 'ACCCT': 246827, 'ACCCC': 376593, 'ACCCG': 142756, 'ACCGA': 93553, 'ACCGT': 89045, 'ACCGC': 203646, 'ACCGG': 155014, 'ACGAA': 45195, 'ACGAT': 57078, 'ACGAC': 117598, 'ACGAG': 205765, 'ACGTA': 25803, 'ACGTT': 34284, 'ACGTC': 91390, 'ACGTG': 165087, 'ACGCA': 104594, 'ACGCT': 148393, 'ACGCC': 202678, 'ACGCG': 73074, 'ACGGA': 143514, 'ACGGT': 67330, 'ACGGC': 193969, 'ACGGG': 144982, 'AGAAA': 297729, 'AGAAT': 130252, 'AGAAC': 244219, 'AGAAG': 571428, 'AGATA': 67774, 'AGATT': 123740, 'AGATC': 251814, 'AGATG': 301168, 'AGACA': 220215, 'AGACT': 150051, 'AGACC': 261946, 'AGACG': 168013, 'AGAGA': 288346, 'AGAGT': 153327, 'AGAGC': 290940, 'AGAGG': 332069, 'AGTAA': 47787, 'AGTAT': 84444, 'AGTAC': 149795, 'AGTAG': 60830, 'AGTTA': 60735, 'AGTTT': 140283, 'AGTTC': 215236, 'AGTTG': 98290, 'AGTCA': 127706, 'AGTCT': 113688, 'AGTCC': 202604, 'AGTCG': 74636, 'AGTGA': 189962, 'AGTGT': 123724, 'AGTGC': 192014, 'AGTGG': 257069, 'AGCAA': 189186, 'AGCAT': 128132, 'AGCAC': 243559, 'AGCAG': 480921, 'AGCTA': 89593, 'AGCTT': 146108, 'AGCTC': 288852, 'AGCTG': 537419, 'AGCCA': 364320, 'AGCCT': 340406, 'AGCCC': 450165, 'AGCCG': 244050, 'AGCGA': 120753, 'AGCGT': 98490, 'AGCGC': 207698, 'AGCGG': 280805, 'AGGAA': 274146, 'AGGAT': 183485, 'AGGAC': 341047, 'AGGAG': 631671, 'AGGTA': 71691, 'AGGTT': 113679, 'AGGTC': 190314, 'AGGTG': 347298, 'AGGCA': 242523, 'AGGCT': 255076, 'AGGCC': 470285, 'AGGCG': 205629, 'AGGGA': 203916, 'AGGGT': 161427, 'AGGGC': 334429, 'AGGGG': 245001, 'TAAAA': 108976, 'TAAAT': 58219, 'TAAAC': 55507, 'TAAAG': 107189, 'TAATA': 27619, 'TAATT': 59782, 'TAATC': 38570, 'TAATG': 63219, 'TAACA': 65364, 'TAACT': 59143, 'TAACC': 51245, 'TAACG': 17628, 'TAAGA': 76913, 'TAAGT': 41155, 'TAAGC': 50952, 'TAAGG': 55280, 'TATAA': 43074, 'TATAT': 54933, 'TATAC': 33908, 'TATAG': 29149, 'TATTA': 45290, 'TATTT': 133678, 'TATTC': 58632, 'TATTG': 62065, 'TATCA': 62382, 'TATCT': 83549, 'TATCC': 64863, 'TATCG': 21421, 'TATGA': 104031, 'TATGT': 81995, 'TATGC': 79704, 'TATGG': 96400, 'TACAA': 160184, 'TACAT': 96313, 'TACAC': 89843, 'TACAG': 126271, 'TACTA': 56679, 'TACTT': 84302, 'TACTC': 63611, 'TACTG': 109896, 'TACCA': 131150, 'TACCT': 136868, 'TACCC': 114319, 'TACCG': 93565, 'TACGA': 94576, 'TACGT': 37715, 'TACGC': 62932, 'TACGG': 71883, 'TAGAA': 61426, 'TAGAT': 37343, 'TAGAC': 46518, 'TAGAG': 81822, 'TAGTA': 28069, 'TAGTT': 35967, 'TAGTC': 38209, 'TAGTG': 58898, 'TAGCA': 51995, 'TAGCT': 62295, 'TAGCC': 68752, 'TAGCG': 23502, 'TAGGA': 63909, 'TAGGT': 31910, 'TAGGC': 45453, 'TAGGG': 48882, 'TTAAA': 105218, 'TTAAT': 58195, 'TTAAC': 57278, 'TTAAG': 66406, 'TTATA': 46879, 'TTATT': 100367, 'TTATC': 52260, 'TTATG': 74022, 'TTACA': 79710, 'TTACT': 72331, 'TTACC': 62807, 'TTACG': 19546, 'TTAGA': 46276, 'TTAGT': 40104, 'TTAGC': 54681, 'TTAGG': 44009, 'TTTAA': 107611, 'TTTAT': 119252, 'TTTAC': 60063, 'TTTAG': 56238, 'TTTTA': 147080, 'TTTTT': 320177, 'TTTTC': 149247, 'TTTTG': 179132, 'TTTCA': 138253, 'TTTCT': 193651, 'TTTCC': 178975, 'TTTCG': 53996, 'TTTGA': 171291, 'TTTGT': 171611, 'TTTGC': 149176, 'TTTGG': 192473, 'TTCAA': 191897, 'TTCAT': 145091, 'TTCAC': 167762, 'TTCAG': 192523, 'TTCTA': 102680, 'TTCTT': 181217, 'TTCTC': 234786, 'TTCTG': 230096, 'TTCCA': 224286, 'TTCCT': 299639, 'TTCCC': 236113, 'TTCCG': 117823, 'TTCGA': 107175, 'TTCGT': 71064, 'TTCGC': 66465, 'TTCGG': 101515, 'TTGAA': 134076, 'TTGAT': 90294, 'TTGAC': 122587, 'TTGAG': 154152, 'TTGTA': 74360, 'TTGTT': 111218, 'TTGTC': 113464, 'TTGTG': 176133, 'TTGCA': 116131, 'TTGCT': 134839, 'TTGCC': 169489, 'TTGCG': 51085, 'TTGGA': 150705, 'TTGGT': 95389, 'TTGGC': 160933, 'TTGGG': 172086, 'TCAAA': 152980, 'TCAAT': 72131, 'TCAAC': 203946, 'TCAAG': 313705, 'TCATA': 48494, 'TCATT': 105271, 'TCATC': 257339, 'TCATG': 211586, 'TCACA': 141921, 'TCACT': 154187, 'TCACC': 293903, 'TCACG': 95927, 'TCAGA': 191905, 'TCAGT': 136190, 'TCAGC': 252690, 'TCAGG': 209350, 'TCTAA': 43290, 'TCTAT': 67969, 'TCTAC': 168941, 'TCTAG': 63161, 'TCTTA': 61699, 'TCTTT': 182436, 'TCTTC': 287083, 'TCTTG': 132616, 'TCTCA': 182992, 'TCTCT': 216611, 'TCTCC': 321628, 'TCTCG': 99302, 'TCTGA': 167833, 'TCTGT': 200630, 'TCTGC': 269676, 'TCTGG': 315326, 'TCCAA': 207815, 'TCCAT': 146724, 'TCCAC': 267785, 'TCCAG': 385373, 'TCCTA': 104678, 'TCCTT': 182936, 'TCCTC': 345949, 'TCCTG': 489834, 'TCCCA': 294945, 'TCCCT': 269156, 'TCCCC': 386339, 'TCCCG': 196499, 'TCCGA': 110963, 'TCCGT': 89443, 'TCCGC': 205701, 'TCCGG': 197537, 'TCGAA': 52748, 'TCGAT': 39132, 'TCGAC': 99093, 'TCGAG': 154102, 'TCGTA': 26100, 'TCGTT': 28919, 'TCGTC': 90560, 'TCGTG': 139777, 'TCGCA': 59096, 'TCGCT': 89107, 'TCGCC': 146146, 'TCGCG': 66916, 'TCGGA': 110792, 'TCGGT': 67730, 'TCGGC': 168769, 'TCGGG': 153354, 'TGAAA': 165294, 'TGAAT': 102172, 'TGAAC': 180373, 'TGAAG': 403105, 'TGATA': 57725, 'TGATT': 89998, 'TGATC': 152626, 'TGATG': 242472, 'TGACA': 181510, 'TGACT': 131814, 'TGACC': 289280, 'TGACG': 111353, 'TGAGA': 192876, 'TGAGT': 134701, 'TGAGC': 275999, 'TGAGG': 324246, 'TGTAA': 86036, 'TGTAT': 79452, 'TGTAC': 108170, 'TGTAG': 65317, 'TGTTA': 66463, 'TGTTT': 168590, 'TGTTC': 158830, 'TGTTG': 132161, 'TGTCA': 155557, 'TGTCT': 171441, 'TGTCC': 253407, 'TGTCG': 76343, 'TGTGA': 211667, 'TGTGT': 195786, 'TGTGC': 243485, 'TGTGG': 338433, 'TGCAA': 146299, 'TGCAT': 107503, 'TGCAC': 186452, 'TGCAG': 433189, 'TGCTA': 77332, 'TGCTT': 151919, 'TGCTC': 221150, 'TGCTG': 539778, 'TGCCA': 298388, 'TGCCT': 322442, 'TGCCC': 432685, 'TGCCG': 197836, 'TGCGA': 83420, 'TGCGT': 82614, 'TGCGC': 184654, 'TGCGG': 198786, 'TGGAA': 222133, 'TGGAT': 180406, 'TGGAC': 353444, 'TGGAG': 577839, 'TGGTA': 79494, 'TGGTT': 117721, 'TGGTC': 216355, 'TGGTG': 404995, 'TGGCA': 260606, 'TGGCT': 312604, 'TGGCC': 529919, 'TGGCG': 220663, 'TGGGA': 288576, 'TGGGT': 190647, 'TGGGC': 424362, 'TGGGG': 378297, 'CAAAA': 187297, 'CAAAT': 119068, 'CAAAC': 144831, 'CAAAG': 240100, 'CAATA': 52474, 'CAATT': 63145, 'CAATC': 69002, 'CAATG': 134850, 'CAACA': 270872, 'CAACT': 144710, 'CAACC': 232191, 'CAACG': 137353, 'CAAGA': 448362, 'CAAGT': 222949, 'CAAGC': 308565, 'CAAGG': 437070, 'CATAA': 49481, 'CATAT': 44095, 'CATAC': 57762, 'CATAG': 47832, 'CATTA': 61496, 'CATTT': 115567, 'CATTC': 115387, 'CATTG': 159026, 'CATCA': 309252, 'CATCT': 253572, 'CATCC': 322388, 'CATCG': 187534, 'CATGA': 219801, 'CATGT': 166088, 'CATGC': 200674, 'CATGG': 321990, 'CACAA': 164977, 'CACAT': 125935, 'CACAC': 190801, 'CACAG': 301844, 'CACTA': 80952, 'CACTT': 138993, 'CACTC': 151140, 'CACTG': 269355, 'CACCA': 390369, 'CACCT': 333134, 'CACCC': 393544, 'CACCG': 208793, 'CACGA': 101132, 'CACGT': 94503, 'CACGC': 201974, 'CACGG': 187689, 'CAGAA': 304398, 'CAGAT': 215704, 'CAGAC': 255138, 'CAGAG': 383099, 'CAGTA': 101299, 'CAGTT': 161279, 'CAGTC': 154441, 'CAGTG': 323040, 'CAGCA': 457715, 'CAGCT': 407176, 'CAGCC': 635937, 'CAGCG': 291174, 'CAGGA': 382175, 'CAGGT': 245774, 'CAGGC': 411969, 'CAGGG': 339888, 'CTAAA': 73714, 'CTAAT': 40310, 'CTAAC': 39283, 'CTAAG': 69929, 'CTATA': 39929, 'CTATT': 68127, 'CTATC': 73848, 'CTATG': 141425, 'CTACA': 202276, 'CTACT': 108808, 'CTACC': 223917, 'CTACG': 164605, 'CTAGA': 63089, 'CTAGT': 41109, 'CTAGC': 56561, 'CTAGG': 58425, 'CTTAA': 61620, 'CTTAT': 60026, 'CTTAC': 52770, 'CTTAG': 57532, 'CTTTA': 66891, 'CTTTT': 178823, 'CTTTC': 178770, 'CTTTG': 231144, 'CTTCA': 301291, 'CTTCT': 285173, 'CTTCC': 418903, 'CTTCG': 160003, 'CTTGA': 124049, 'CTTGT': 115439, 'CTTGC': 139500, 'CTTGG': 180881, 'CTCAA': 221474, 'CTCAT': 179138, 'CTCAC': 229342, 'CTCAG': 302481, 'CTCTA': 96173, 'CTCTT': 193933, 'CTCTC': 249353, 'CTCTG': 342784, 'CTCCA': 385681, 'CTCCT': 386794, 'CTCCC': 449007, 'CTCCG': 228075, 'CTCGA': 72683, 'CTCGT': 70294, 'CTCGC': 129271, 'CTCGG': 214192, 'CTGAA': 279692, 'CTGAT': 165836, 'CTGAC': 230296, 'CTGAG': 343586, 'CTGTA': 120210, 'CTGTT': 162494, 'CTGTC': 244753, 'CTGTG': 371481, 'CTGCA': 400996, 'CTGCT': 467073, 'CTGCC': 587733, 'CTGCG': 277477, 'CTGGA': 577523, 'CTGGT': 314324, 'CTGGC': 524839, 'CTGGG': 570076, 'CCAAA': 200306, 'CCAAT': 93920, 'CCAAC': 255477, 'CCAAG': 458396, 'CCATA': 69648, 'CCATT': 133764, 'CCATC': 357302, 'CCATG': 327908, 'CCACA': 288940, 'CCACT': 222227, 'CCACC': 528831, 'CCACG': 235275, 'CCAGA': 415119, 'CCAGT': 248882, 'CCAGC': 653694, 'CCAGG': 555278, 'CCTAA': 69373, 'CCTAT': 101361, 'CCTAC': 217396, 'CCTAG': 76281, 'CCTTA': 74051, 'CCTTT': 181748, 'CCTTC': 382063, 'CCTTG': 194213, 'CCTCA': 365175, 'CCTCT': 315818, 'CCTCC': 561546, 'CCTCG': 203827, 'CCTGA': 345567, 'CCTGT': 270245, 'CCTGC': 640535, 'CCTGG': 797271, 'CCCAA': 272638, 'CCCAT': 208284, 'CCCAC': 401547, 'CCCAG': 663895, 'CCCTA': 135097, 'CCCTT': 235920, 'CCCTC': 404073, 'CCCTG': 620027, 'CCCCA': 544707, 'CCCCT': 436027, 'CCCCC': 660037, 'CCCCG': 377094, 'CCCGA': 212847, 'CCCGT': 139428, 'CCCGC': 341778, 'CCCGG': 390681, 'CCGAA': 85931, 'CCGAT': 60763, 'CCGAC': 180605, 'CCGAG': 307908, 'CCGTA': 39790, 'CCGTT': 54935, 'CCGTC': 158285, 'CCGTG': 216209, 'CCGCA': 237860, 'CCGCT': 232675, 'CCGCC': 496545, 'CCGCG': 233962, 'CCGGA': 233807, 'CCGGT': 87837, 'CCGGC': 353424, 'CCGGG': 397141, 'CGAAA': 44969, 'CGAAT': 44437, 'CGAAC': 55245, 'CGAAG': 95801, 'CGATA': 24008, 'CGATT': 42787, 'CGATC': 42974, 'CGATG': 106371, 'CGACA': 134116, 'CGACT': 95442, 'CGACC': 164098, 'CGACG': 123905, 'CGAGA': 207236, 'CGAGT': 125935, 'CGAGC': 215541, 'CGAGG': 333311, 'CGTAA': 25443, 'CGTAT': 19877, 'CGTAC': 41025, 'CGTAG': 28186, 'CGTTA': 13496, 'CGTTT': 45371, 'CGTTC': 53831, 'CGTTG': 53463, 'CGTCA': 131450, 'CGTCT': 126917, 'CGTCC': 155816, 'CGTCG': 62176, 'CGTGA': 115438, 'CGTGT': 107258, 'CGTGC': 197532, 'CGTGG': 293872, 'CGCAA': 144273, 'CGCAT': 112289, 'CGCAC': 127542, 'CGCAG': 191472, 'CGCTA': 80471, 'CGCTT': 110071, 'CGCTC': 160508, 'CGCTG': 316228, 'CGCCA': 283252, 'CGCCT': 274172, 'CGCCC': 329184, 'CGCCG': 276774, 'CGCGA': 93686, 'CGCGT': 73716, 'CGCGC': 178505, 'CGCGG': 208616, 'CGGAA': 164647, 'CGGAT': 83901, 'CGGAC': 161391, 'CGGAG': 310512, 'CGGTA': 33168, 'CGGTT': 52304, 'CGGTC': 86998, 'CGGTG': 149863, 'CGGCA': 238490, 'CGGCT': 229931, 'CGGCC': 433371, 'CGGCG': 298108, 'CGGGA': 254492, 'CGGGT': 116781, 'CGGGC': 311493, 'CGGGG': 309155, 'GAAAA': 202506, 'GAAAT': 126886, 'GAAAC': 172812, 'GAAAG': 207173, 'GAATA': 66348, 'GAATT': 95427, 'GAATC': 86145, 'GAATG': 142736, 'GAACA': 195447, 'GAACT': 155859, 'GAACC': 192428, 'GAACG': 114140, 'GAAGA': 509076, 'GAAGT': 198193, 'GAAGC': 373124, 'GAAGG': 380886, 'GATAA': 60699, 'GATAT': 54955, 'GATAC': 51650, 'GATAG': 42400, 'GATTA': 54634, 'GATTT': 105076, 'GATTC': 111020, 'GATTG': 97759, 'GATCA': 161293, 'GATCT': 129022, 'GATCC': 216142, 'GATCG': 86123, 'GATGA': 321225, 'GATGT': 146672, 'GATGC': 189732, 'GATGG': 264819, 'GACAA': 233337, 'GACAT': 191975, 'GACAC': 173071, 'GACAG': 262656, 'GACTA': 75856, 'GACTT': 145566, 'GACTC': 190586, 'GACTG': 191494, 'GACCA': 335142, 'GACCT': 269284, 'GACCC': 338174, 'GACCG': 157250, 'GACGA': 154504, 'GACGT': 104434, 'GACGC': 177016, 'GACGG': 192156, 'GAGAA': 408551, 'GAGAT': 224086, 'GAGAC': 255227, 'GAGAG': 292513, 'GAGTA': 98559, 'GAGTT': 151683, 'GAGTC': 166029, 'GAGTG': 215448, 'GAGCA': 305242, 'GAGCT': 337988, 'GAGCC': 413639, 'GAGCG': 250930, 'GAGGA': 629754, 'GAGGT': 246020, 'GAGGC': 449592, 'GAGGG': 333018, 'GTAAA': 64977, 'GTAAT': 48150, 'GTAAC': 50305, 'GTAAG': 41616, 'GTATA': 28339, 'GTATT': 65982, 'GTATC': 51323, 'GTATG': 92060, 'GTACA': 116709, 'GTACT': 76827, 'GTACC': 130154, 'GTACG': 56534, 'GTAGA': 68658, 'GTAGT': 41484, 'GTAGC': 58425, 'GTAGG': 46012, 'GTTAA': 48274, 'GTTAT': 40969, 'GTTAC': 50669, 'GTTAG': 40320, 'GTTTA': 54810, 'GTTTT': 128660, 'GTTTC': 113435, 'GTTTG': 171476, 'GTTCA': 151145, 'GTTCT': 160631, 'GTTCC': 169510, 'GTTCG': 82527, 'GTTGA': 76479, 'GTTGT': 92231, 'GTTGC': 94128, 'GTTGG': 129578, 'GTCAA': 137224, 'GTCAT': 130399, 'GTCAC': 157889, 'GTCAG': 176493, 'GTCTA': 70290, 'GTCTT': 132887, 'GTCTC': 182611, 'GTCTG': 207096, 'GTCCA': 212183, 'GTCCT': 231111, 'GTCCC': 279594, 'GTCCG': 117998, 'GTCGA': 56485, 'GTCGT': 75418, 'GTCGC': 89519, 'GTCGG': 109715, 'GTGAA': 229891, 'GTGAT': 120742, 'GTGAC': 205327, 'GTGAG': 196563, 'GTGTA': 71587, 'GTGTT': 142573, 'GTGTC': 155105, 'GTGTG': 248600, 'GTGCA': 212293, 'GTGCT': 242493, 'GTGCC': 284034, 'GTGCG': 151528, 'GTGGA': 382915, 'GTGGT': 253522, 'GTGGC': 389773, 'GTGGG': 337402, 'GCAAA': 152023, 'GCAAT': 77524, 'GCAAC': 146374, 'GCAAG': 316633, 'GCATA': 33977, 'GCATT': 101405, 'GCATC': 215093, 'GCATG': 175296, 'GCACA': 180726, 'GCACT': 156118, 'GCACC': 307757, 'GCACG': 153942, 'GCAGA': 327734, 'GCAGT': 214805, 'GCAGC': 584474, 'GCAGG': 404760, 'GCTAA': 59988, 'GCTAT': 89350, 'GCTAC': 174787, 'GCTAG': 53293, 'GCTTA': 48725, 'GCTTT': 154270, 'GCTTC': 279444, 'GCTTG': 127951, 'GCTCA': 258950, 'GCTCT': 225896, 'GCTCC': 359533, 'GCTCG': 112865, 'GCTGA': 350633, 'GCTGT': 284681, 'GCTGC': 618542, 'GCTGG': 639486, 'GCCAA': 292044, 'GCCAT': 312258, 'GCCAC': 386692, 'GCCAG': 462550, 'GCCTA': 129813, 'GCCTT': 255782, 'GCCTC': 437397, 'GCCTG': 544067, 'GCCCA': 437218, 'GCCCT': 443226, 'GCCCC': 604420, 'GCCCG': 367961, 'GCCGA': 218464, 'GCCGT': 151799, 'GCCGC': 448036, 'GCCGG': 328289, 'GCGAA': 56321, 'GCGAT': 59085, 'GCGAC': 120293, 'GCGAG': 214117, 'GCGTA': 22747, 'GCGTT': 47730, 'GCGTC': 135951, 'GCGTG': 194389, 'GCGCA': 173769, 'GCGCT': 196930, 'GCGCC': 319563, 'GCGCG': 178741, 'GCGGA': 228614, 'GCGGT': 99023, 'GCGGC': 481992, 'GCGGG': 296223, 'GGAAA': 200833, 'GGAAT': 113991, 'GGAAC': 178183, 'GGAAG': 394533, 'GGATA': 60283, 'GGATT': 112076, 'GGATC': 140583, 'GGATG': 272597, 'GGACA': 325832, 'GGACT': 226801, 'GGACC': 387502, 'GGACG': 225406, 'GGAGA': 492145, 'GGAGT': 219166, 'GGAGC': 522746, 'GGAGG': 667915, 'GGTAA': 45993, 'GGTAT': 54330, 'GGTAC': 81647, 'GGTAG': 60221, 'GGTTA': 39814, 'GGTTT': 115061, 'GGTTC': 135136, 'GGTTG': 107236, 'GGTCA': 185794, 'GGTCT': 179657, 'GGTCC': 229461, 'GGTCG': 117546, 'GGTGA': 234118, 'GGTGT': 191647, 'GGTGC': 258744, 'GGTGG': 475790, 'GGCAA': 213798, 'GGCAT': 178002, 'GGCAC': 239920, 'GGCAG': 427631, 'GGCTA': 129981, 'GGCTT': 202541, 'GGCTC': 289196, 'GGCTG': 502769, 'GGCCA': 508899, 'GGCCT': 433836, 'GGCCC': 644128, 'GGCCG': 428371, 'GGCGA': 150983, 'GGCGT': 146185, 'GGCGC': 297611, 'GGCGG': 414206, 'GGGAA': 227257, 'GGGAT': 138221, 'GGGAC': 309803, 'GGGAG': 379917, 'GGGTA': 58692, 'GGGTT': 113483, 'GGGTC': 217469, 'GGGTG': 256868, 'GGGCA': 319661, 'GGGCT': 327217, 'GGGCC': 582477, 'GGGCG': 280390, 'GGGGA': 310047, 'GGGGT': 177831, 'GGGGC': 442758, 'GGGGG': 311233}, 'overrepresented_sequences': {}} {'passed_filter_reads': 7053940, 'low_quality_reads': 158028, 'too_many_N_reads': 1560, 'too_short_reads': 86, 'too_long_reads': 0} fastp -i /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045020_1.fastq.gz -I /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045020_2.fastq.gz -o /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045020.trimmed_1.fastq.gz -O /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045020.trimmed_2.fastq.gz -l 25 --adapter_fasta /home/ubuntu/workspace/rnaseq/team_exercise/references/illumina_multiplex.fa --trim_tail1 5 --trim_tail2 5 --json /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045020.fastp.json --html /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045020.fastp.html SRR10045021_1 {'adapter_trimmed_reads': 47674, 'adapter_trimmed_bases': 294070, 'read1_adapter_sequence': 'unspecified', 'read2_adapter_sequence': 'unspecified', 'read1_adapter_counts': {'A': 745, 'C': 904, 'G': 963, 'T': 735, 'AG': 243, 'CC': 248, 'GG': 332, 'others': 19667}, 'read2_adapter_counts': {'A': 872, 'C': 959, 'G': 847, 'T': 669, 'AG': 248, 'CC': 273, 'GG': 244, 'TG': 243, 'others': 19482}} {'rate': 0.0830907} {'peak': 85, 'unknown': 980831, 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264552, 'ACCTG': 407689, 'ACCCA': 283387, 'ACCCT': 255474, 'ACCCC': 426886, 'ACCCG': 155122, 'ACCGA': 97730, 'ACCGT': 92625, 'ACCGC': 217721, 'ACCGG': 167716, 'ACGAA': 48378, 'ACGAT': 57878, 'ACGAC': 123291, 'ACGAG': 211285, 'ACGTA': 26569, 'ACGTT': 35613, 'ACGTC': 95182, 'ACGTG': 166544, 'ACGCA': 106137, 'ACGCT': 151695, 'ACGCC': 215084, 'ACGCG': 77111, 'ACGGA': 144149, 'ACGGT': 68346, 'ACGGC': 202152, 'ACGGG': 152453, 'AGAAA': 301299, 'AGAAT': 129358, 'AGAAC': 250741, 'AGAAG': 576675, 'AGATA': 66537, 'AGATT': 123297, 'AGATC': 265018, 'AGATG': 301481, 'AGACA': 221525, 'AGACT': 148200, 'AGACC': 270327, 'AGACG': 171488, 'AGAGA': 285365, 'AGAGT': 149384, 'AGAGC': 294348, 'AGAGG': 339270, 'AGTAA': 47666, 'AGTAT': 81553, 'AGTAC': 151510, 'AGTAG': 60627, 'AGTTA': 59361, 'AGTTT': 140385, 'AGTTC': 218987, 'AGTTG': 96302, 'AGTCA': 128010, 'AGTCT': 111922, 'AGTCC': 208353, 'AGTCG': 75516, 'AGTGA': 189936, 'AGTGT': 121684, 'AGTGC': 192813, 'AGTGG': 256616, 'AGCAA': 192975, 'AGCAT': 128775, 'AGCAC': 247734, 'AGCAG': 483556, 'AGCTA': 89866, 'AGCTT': 147029, 'AGCTC': 287879, 'AGCTG': 545288, 'AGCCA': 368813, 'AGCCT': 342271, 'AGCCC': 492270, 'AGCCG': 257957, 'AGCGA': 125373, 'AGCGT': 99887, 'AGCGC': 216065, 'AGCGG': 293125, 'AGGAA': 279905, 'AGGAT': 183752, 'AGGAC': 350023, 'AGGAG': 642498, 'AGGTA': 71198, 'AGGTT': 115786, 'AGGTC': 195930, 'AGGTG': 347131, 'AGGCA': 243431, 'AGGCT': 253907, 'AGGCC': 493649, 'AGGCG': 212177, 'AGGGA': 205703, 'AGGGT': 162718, 'AGGGC': 345685, 'AGGGG': 261053, 'TAAAA': 108784, 'TAAAT': 57317, 'TAAAC': 56303, 'TAAAG': 107261, 'TAATA': 26463, 'TAATT': 58159, 'TAATC': 37736, 'TAATG': 62435, 'TAACA': 64291, 'TAACT': 57616, 'TAACC': 52730, 'TAACG': 18914, 'TAAGA': 75792, 'TAAGT': 40034, 'TAAGC': 53573, 'TAAGG': 57004, 'TATAA': 41458, 'TATAT': 52979, 'TATAC': 33866, 'TATAG': 27469, 'TATTA': 44323, 'TATTT': 128952, 'TATTC': 57595, 'TATTG': 60880, 'TATCA': 63361, 'TATCT': 81807, 'TATCC': 66944, 'TATCG': 21872, 'TATGA': 102703, 'TATGT': 82268, 'TATGC': 81827, 'TATGG': 96111, 'TACAA': 160300, 'TACAT': 95165, 'TACAC': 91563, 'TACAG': 126237, 'TACTA': 55108, 'TACTT': 83459, 'TACTC': 64786, 'TACTG': 109979, 'TACCA': 131586, 'TACCT': 140733, 'TACCC': 122964, 'TACCG': 97801, 'TACGA': 95767, 'TACGT': 38312, 'TACGC': 65960, 'TACGG': 72742, 'TAGAA': 60818, 'TAGAT': 36589, 'TAGAC': 46849, 'TAGAG': 81326, 'TAGTA': 27247, 'TAGTT': 34576, 'TAGTC': 37711, 'TAGTG': 56604, 'TAGCA': 51314, 'TAGCT': 61301, 'TAGCC': 70101, 'TAGCG': 24778, 'TAGGA': 63838, 'TAGGT': 31714, 'TAGGC': 46807, 'TAGGG': 50599, 'TTAAA': 102263, 'TTAAT': 57605, 'TTAAC': 55971, 'TTAAG': 66947, 'TTATA': 45115, 'TTATT': 96538, 'TTATC': 52789, 'TTATG': 73002, 'TTACA': 78133, 'TTACT': 70140, 'TTACC': 64013, 'TTACG': 20690, 'TTAGA': 44724, 'TTAGT': 39140, 'TTAGC': 54439, 'TTAGG': 44485, 'TTTAA': 104662, 'TTTAT': 116671, 'TTTAC': 59466, 'TTTAG': 54039, 'TTTTA': 139137, 'TTTTT': 304756, 'TTTTC': 148114, 'TTTTG': 175875, 'TTTCA': 136081, 'TTTCT': 190373, 'TTTCC': 188339, 'TTTCG': 55924, 'TTTGA': 171274, 'TTTGT': 167804, 'TTTGC': 149053, 'TTTGG': 196023, 'TTCAA': 193818, 'TTCAT': 146209, 'TTCAC': 170779, 'TTCAG': 193571, 'TTCTA': 102618, 'TTCTT': 178678, 'TTCTC': 233061, 'TTCTG': 230135, 'TTCCA': 227552, 'TTCCT': 304364, 'TTCCC': 256148, 'TTCCG': 123103, 'TTCGA': 109575, 'TTCGT': 72092, 'TTCGC': 69058, 'TTCGG': 105538, 'TTGAA': 133082, 'TTGAT': 89522, 'TTGAC': 125520, 'TTGAG': 153929, 'TTGTA': 71704, 'TTGTT': 107267, 'TTGTC': 112139, 'TTGTG': 175477, 'TTGCA': 115416, 'TTGCT': 131161, 'TTGCC': 175199, 'TTGCG': 52766, 'TTGGA': 150848, 'TTGGT': 93590, 'TTGGC': 165682, 'TTGGG': 175145, 'TCAAA': 151768, 'TCAAT': 71909, 'TCAAC': 208667, 'TCAAG': 319920, 'TCATA': 48650, 'TCATT': 104813, 'TCATC': 263610, 'TCATG': 212027, 'TCACA': 139659, 'TCACT': 153008, 'TCACC': 303771, 'TCACG': 98404, 'TCAGA': 192464, 'TCAGT': 131884, 'TCAGC': 254067, 'TCAGG': 209834, 'TCTAA': 42098, 'TCTAT': 67920, 'TCTAC': 171158, 'TCTAG': 61879, 'TCTTA': 60056, 'TCTTT': 179299, 'TCTTC': 287924, 'TCTTG': 130386, 'TCTCA': 177317, 'TCTCT': 211502, 'TCTCC': 329466, 'TCTCG': 100290, 'TCTGA': 163852, 'TCTGT': 196648, 'TCTGC': 275009, 'TCTGG': 316530, 'TCCAA': 214093, 'TCCAT': 149683, 'TCCAC': 276130, 'TCCAG': 389940, 'TCCTA': 106504, 'TCCTT': 185724, 'TCCTC': 355264, 'TCCTG': 499308, 'TCCCA': 302408, 'TCCCT': 281153, 'TCCCC': 436604, 'TCCCG': 209957, 'TCCGA': 116468, 'TCCGT': 91379, 'TCCGC': 215736, 'TCCGG': 208487, 'TCGAA': 53315, 'TCGAT': 39763, 'TCGAC': 104132, 'TCGAG': 157884, 'TCGTA': 26973, 'TCGTT': 29016, 'TCGTC': 93008, 'TCGTG': 142982, 'TCGCA': 60683, 'TCGCT': 90141, 'TCGCC': 155561, 'TCGCG': 70134, 'TCGGA': 112075, 'TCGGT': 68474, 'TCGGC': 172722, 'TCGGG': 157904, 'TGAAA': 164842, 'TGAAT': 100105, 'TGAAC': 182044, 'TGAAG': 410119, 'TGATA': 56667, 'TGATT': 89029, 'TGATC': 155110, 'TGATG': 244140, 'TGACA': 181495, 'TGACT': 133209, 'TGACC': 300002, 'TGACG': 113304, 'TGAGA': 191041, 'TGAGT': 131602, 'TGAGC': 279588, 'TGAGG': 329490, 'TGTAA': 83486, 'TGTAT': 77735, 'TGTAC': 109115, 'TGTAG': 63529, 'TGTTA': 62724, 'TGTTT': 164813, 'TGTTC': 159248, 'TGTTG': 130877, 'TGTCA': 152537, 'TGTCT': 169327, 'TGTCC': 259110, 'TGTCG': 79561, 'TGTGA': 210837, 'TGTGT': 192124, 'TGTGC': 244316, 'TGTGG': 338945, 'TGCAA': 147226, 'TGCAT': 108369, 'TGCAC': 188984, 'TGCAG': 432311, 'TGCTA': 77822, 'TGCTT': 150658, 'TGCTC': 225066, 'TGCTG': 537875, 'TGCCA': 303966, 'TGCCT': 326136, 'TGCCC': 466523, 'TGCCG': 207495, 'TGCGA': 85206, 'TGCGT': 82837, 'TGCGC': 191923, 'TGCGG': 206638, 'TGGAA': 221129, 'TGGAT': 180272, 'TGGAC': 359321, 'TGGAG': 580306, 'TGGTA': 79673, 'TGGTT': 116596, 'TGGTC': 217574, 'TGGTG': 407789, 'TGGCA': 261996, 'TGGCT': 310525, 'TGGCC': 549426, 'TGGCG': 228786, 'TGGGA': 288792, 'TGGGT': 188233, 'TGGGC': 432957, 'TGGGG': 392438, 'CAAAA': 193312, 'CAAAT': 119352, 'CAAAC': 150681, 'CAAAG': 244896, 'CAATA': 54350, 'CAATT': 62331, 'CAATC': 70636, 'CAATG': 138263, 'CAACA': 276685, 'CAACT': 146729, 'CAACC': 247757, 'CAACG': 143458, 'CAAGA': 458234, 'CAAGT': 227108, 'CAAGC': 320191, 'CAAGG': 446244, 'CATAA': 50469, 'CATAT': 43699, 'CATAC': 58339, 'CATAG': 48092, 'CATTA': 61488, 'CATTT': 115636, 'CATTC': 117227, 'CATTG': 159857, 'CATCA': 314770, 'CATCT': 256019, 'CATCC': 339564, 'CATCG': 193724, 'CATGA': 221505, 'CATGT': 166597, 'CATGC': 204214, 'CATGG': 330070, 'CACAA': 167233, 'CACAT': 125533, 'CACAC': 195647, 'CACAG': 302916, 'CACTA': 82304, 'CACTT': 141969, 'CACTC': 152355, 'CACTG': 268988, 'CACCA': 401146, 'CACCT': 339544, 'CACCC': 439453, 'CACCG': 223755, 'CACGA': 105930, 'CACGT': 95617, 'CACGC': 209380, 'CACGG': 194108, 'CAGAA': 307658, 'CAGAT': 219996, 'CAGAC': 259696, 'CAGAG': 381941, 'CAGTA': 101121, 'CAGTT': 164240, 'CAGTC': 154560, 'CAGTG': 322770, 'CAGCA': 462146, 'CAGCT': 405052, 'CAGCC': 662737, 'CAGCG': 301510, 'CAGGA': 384977, 'CAGGT': 250391, 'CAGGC': 422584, 'CAGGG': 351265, 'CTAAA': 74547, 'CTAAT': 39171, 'CTAAC': 40324, 'CTAAG': 70969, 'CTATA': 39401, 'CTATT': 68279, 'CTATC': 76104, 'CTATG': 143531, 'CTACA': 204466, 'CTACT': 108711, 'CTACC': 232486, 'CTACG': 166420, 'CTAGA': 63611, 'CTAGT': 40012, 'CTAGC': 56993, 'CTAGG': 60013, 'CTTAA': 60918, 'CTTAT': 59066, 'CTTAC': 53782, 'CTTAG': 59063, 'CTTTA': 67274, 'CTTTT': 176302, 'CTTTC': 181545, 'CTTTG': 234144, 'CTTCA': 305365, 'CTTCT': 285963, 'CTTCC': 438665, 'CTTCG': 164954, 'CTTGA': 123995, 'CTTGT': 114091, 'CTTGC': 143631, 'CTTGG': 182220, 'CTCAA': 219871, 'CTCAT': 178294, 'CTCAC': 232264, 'CTCAG': 298810, 'CTCTA': 94652, 'CTCTT': 191086, 'CTCTC': 251839, 'CTCTG': 339383, 'CTCCA': 392485, 'CTCCT': 388954, 'CTCCC': 492335, 'CTCCG': 241662, 'CTCGA': 76391, 'CTCGT': 70616, 'CTCGC': 135348, 'CTCGG': 215853, 'CTGAA': 282658, 'CTGAT': 166757, 'CTGAC': 235532, 'CTGAG': 347964, 'CTGTA': 119110, 'CTGTT': 162931, 'CTGTC': 248453, 'CTGTG': 368495, 'CTGCA': 401172, 'CTGCT': 468799, 'CTGCC': 624753, 'CTGCG': 288188, 'CTGGA': 578887, 'CTGGT': 315627, 'CTGGC': 540388, 'CTGGG': 586243, 'CCAAA': 209348, 'CCAAT': 98063, 'CCAAC': 270809, 'CCAAG': 477574, 'CCATA': 71627, 'CCATT': 138291, 'CCATC': 372265, 'CCATG': 336487, 'CCACA': 295307, 'CCACT': 225109, 'CCACC': 575135, 'CCACG': 245594, 'CCAGA': 423379, 'CCAGT': 257386, 'CCAGC': 676235, 'CCAGG': 573697, 'CCTAA': 72511, 'CCTAT': 105276, 'CCTAC': 224553, 'CCTAG': 79441, 'CCTTA': 76554, 'CCTTT': 189000, 'CCTTC': 402279, 'CCTTG': 199976, 'CCTCA': 366835, 'CCTCT': 315357, 'CCTCC': 601402, 'CCTCG': 211025, 'CCTGA': 353444, 'CCTGT': 273851, 'CCTGC': 668336, 'CCTGG': 820245, 'CCCAA': 294031, 'CCCAT': 220040, 'CCCAC': 440936, 'CCCAG': 695170, 'CCCTA': 144662, 'CCCTT': 257530, 'CCCTC': 435024, 'CCCTG': 652585, 'CCCCA': 597144, 'CCCCT': 481305, 'CCCCC': 970303, 'CCCCG': 440074, 'CCCGA': 226208, 'CCCGT': 149304, 'CCCGC': 388563, 'CCCGG': 435176, 'CCGAA': 92052, 'CCGAT': 63669, 'CCGAC': 194227, 'CCGAG': 320350, 'CCGTA': 42088, 'CCGTT': 59272, 'CCGTC': 166562, 'CCGTG': 225654, 'CCGCA': 250906, 'CCGCT': 241227, 'CCGCC': 552580, 'CCGCG': 248593, 'CCGGA': 246763, 'CCGGT': 94971, 'CCGGC': 388880, 'CCGGG': 430242, 'CGAAA': 47733, 'CGAAT': 45561, 'CGAAC': 58830, 'CGAAG': 101456, 'CGATA': 24426, 'CGATT': 44335, 'CGATC': 45777, 'CGATG': 108334, 'CGACA': 136913, 'CGACT': 98485, 'CGACC': 176206, 'CGACG': 131714, 'CGAGA': 211546, 'CGAGT': 129014, 'CGAGC': 226653, 'CGAGG': 343601, 'CGTAA': 26519, 'CGTAT': 19891, 'CGTAC': 44800, 'CGTAG': 29411, 'CGTTA': 14408, 'CGTTT': 47435, 'CGTTC': 56858, 'CGTTG': 54742, 'CGTCA': 134787, 'CGTCT': 126893, 'CGTCC': 165613, 'CGTCG': 65442, 'CGTGA': 117448, 'CGTGT': 107715, 'CGTGC': 202627, 'CGTGG': 302510, 'CGCAA': 149948, 'CGCAT': 115401, 'CGCAC': 136215, 'CGCAG': 198773, 'CGCTA': 82193, 'CGCTT': 113062, 'CGCTC': 168308, 'CGCTG': 323677, 'CGCCA': 295440, 'CGCCT': 277096, 'CGCCC': 384210, 'CGCCG': 298473, 'CGCGA': 97452, 'CGCGT': 75563, 'CGCGC': 186440, 'CGCGG': 218998, 'CGGAA': 171342, 'CGGAT': 85971, 'CGGAC': 170140, 'CGGAG': 320453, 'CGGTA': 34888, 'CGGTT': 55396, 'CGGTC': 93489, 'CGGTG': 152329, 'CGGCA': 247758, 'CGGCT': 233579, 'CGGCC': 468891, 'CGGCG': 311533, 'CGGGA': 261885, 'CGGGT': 120566, 'CGGGC': 331382, 'CGGGG': 336949, 'GAAAA': 207334, 'GAAAT': 125706, 'GAAAC': 175508, 'GAAAG': 208497, 'GAATA': 64933, 'GAATT': 94498, 'GAATC': 87151, 'GAATG': 144397, 'GAACA': 197033, 'GAACT': 156999, 'GAACC': 201650, 'GAACG': 117683, 'GAAGA': 515337, 'GAAGT': 197919, 'GAAGC': 384635, 'GAAGG': 392383, 'GATAA': 60411, 'GATAT': 53240, 'GATAC': 52891, 'GATAG': 43473, 'GATTA': 54085, 'GATTT': 105634, 'GATTC': 111017, 'GATTG': 97348, 'GATCA': 163851, 'GATCT': 129553, 'GATCC': 224063, 'GATCG': 89824, 'GATGA': 324430, 'GATGT': 144723, 'GATGC': 193667, 'GATGG': 268352, 'GACAA': 238878, 'GACAT': 191266, 'GACAC': 178160, 'GACAG': 266035, 'GACTA': 76664, 'GACTT': 147345, 'GACTC': 192568, 'GACTG': 193683, 'GACCA': 345363, 'GACCT': 274305, 'GACCC': 358871, 'GACCG': 167586, 'GACGA': 159367, 'GACGT': 106766, 'GACGC': 185256, 'GACGG': 197631, 'GAGAA': 409342, 'GAGAT': 227025, 'GAGAC': 258374, 'GAGAG': 293387, 'GAGTA': 97486, 'GAGTT': 151249, 'GAGTC': 169181, 'GAGTG': 213060, 'GAGCA': 307128, 'GAGCT': 335198, 'GAGCC': 436260, 'GAGCG': 259931, 'GAGGA': 638722, 'GAGGT': 245561, 'GAGGC': 459080, 'GAGGG': 350463, 'GTAAA': 65453, 'GTAAT': 46807, 'GTAAC': 50263, 'GTAAG': 41992, 'GTATA': 27281, 'GTATT': 63383, 'GTATC': 51206, 'GTATG': 91304, 'GTACA': 115987, 'GTACT': 78550, 'GTACC': 135656, 'GTACG': 58626, 'GTAGA': 67636, 'GTAGT': 39734, 'GTAGC': 59497, 'GTAGG': 46771, 'GTTAA': 48681, 'GTTAT': 39448, 'GTTAC': 50061, 'GTTAG': 40052, 'GTTTA': 54250, 'GTTTT': 126071, 'GTTTC': 117624, 'GTTTG': 170868, 'GTTCA': 152789, 'GTTCT': 159665, 'GTTCC': 176514, 'GTTCG': 85637, 'GTTGA': 75894, 'GTTGT': 90303, 'GTTGC': 94518, 'GTTGG': 131012, 'GTCAA': 138970, 'GTCAT': 130872, 'GTCAC': 160188, 'GTCAG': 176984, 'GTCTA': 69731, 'GTCTT': 132250, 'GTCTC': 181276, 'GTCTG': 206843, 'GTCCA': 214805, 'GTCCT': 237440, 'GTCCC': 296771, 'GTCCG': 123536, 'GTCGA': 57537, 'GTCGT': 77224, 'GTCGC': 92527, 'GTCGG': 113268, 'GTGAA': 233076, 'GTGAT': 119568, 'GTGAC': 209527, 'GTGAG': 195527, 'GTGTA': 70501, 'GTGTT': 139883, 'GTGTC': 156715, 'GTGTG': 246957, 'GTGCA': 213322, 'GTGCT': 237770, 'GTGCC': 293310, 'GTGCG': 156138, 'GTGGA': 381865, 'GTGGT': 250776, 'GTGGC': 395408, 'GTGGG': 345434, 'GCAAA': 154991, 'GCAAT': 80505, 'GCAAC': 150046, 'GCAAG': 325611, 'GCATA': 34276, 'GCATT': 101279, 'GCATC': 221053, 'GCATG': 178939, 'GCACA': 180543, 'GCACT': 156346, 'GCACC': 325843, 'GCACG': 156237, 'GCAGA': 328355, 'GCAGT': 212523, 'GCAGC': 594605, 'GCAGG': 414766, 'GCTAA': 61597, 'GCTAT': 89473, 'GCTAC': 177947, 'GCTAG': 53508, 'GCTTA': 49166, 'GCTTT': 154956, 'GCTTC': 285243, 'GCTTG': 128196, 'GCTCA': 255972, 'GCTCT': 222596, 'GCTCC': 376332, 'GCTCG': 113824, 'GCTGA': 353851, 'GCTGT': 284556, 'GCTGC': 627437, 'GCTGG': 649922, 'GCCAA': 303373, 'GCCAT': 318246, 'GCCAC': 399308, 'GCCAG': 476812, 'GCCTA': 131937, 'GCCTT': 262799, 'GCCTC': 444532, 'GCCTG': 553519, 'GCCCA': 462739, 'GCCCT': 465250, 'GCCCC': 700483, 'GCCCG': 398390, 'GCCGA': 226962, 'GCCGT': 158253, 'GCCGC': 474935, 'GCCGG': 356804, 'GCGAA': 59822, 'GCGAT': 60876, 'GCGAC': 124835, 'GCGAG': 222262, 'GCGTA': 24556, 'GCGTT': 49436, 'GCGTC': 137942, 'GCGTG': 196329, 'GCGCA': 178495, 'GCGCT': 199507, 'GCGCC': 341799, 'GCGCG': 182206, 'GCGGA': 237452, 'GCGGT': 102157, 'GCGGC': 495952, 'GCGGG': 316934, 'GGAAA': 204209, 'GGAAT': 115165, 'GGAAC': 184014, 'GGAAG': 402102, 'GGATA': 61474, 'GGATT': 111882, 'GGATC': 142481, 'GGATG': 275462, 'GGACA': 329747, 'GGACT': 227433, 'GGACC': 405600, 'GGACG': 232917, 'GGAGA': 497830, 'GGAGT': 217378, 'GGAGC': 535151, 'GGAGG': 681766, 'GGTAA': 46690, 'GGTAT': 54317, 'GGTAC': 84290, 'GGTAG': 60470, 'GGTTA': 41085, 'GGTTT': 117490, 'GGTTC': 139151, 'GGTTG': 108730, 'GGTCA': 186560, 'GGTCT': 177933, 'GGTCC': 244484, 'GGTCG': 120415, 'GGTGA': 236340, 'GGTGT': 190117, 'GGTGC': 261935, 'GGTGG': 477415, 'GGCAA': 220824, 'GGCAT': 180973, 'GGCAC': 249817, 'GGCAG': 434135, 'GGCTA': 131158, 'GGCTT': 206473, 'GGCTC': 290512, 'GGCTG': 506119, 'GGCCA': 520146, 'GGCCT': 441222, 'GGCCC': 708085, 'GGCCG': 454199, 'GGCGA': 155809, 'GGCGT': 147752, 'GGCGC': 309029, 'GGCGG': 435261, 'GGGAA': 234138, 'GGGAT': 139989, 'GGGAC': 318455, 'GGGAG': 388262, 'GGGTA': 60037, 'GGGTT': 117934, 'GGGTC': 222711, 'GGGTG': 257884, 'GGGCA': 327182, 'GGGCT': 327781, 'GGGCC': 623428, 'GGGCG': 294440, 'GGGGA': 321087, 'GGGGT': 184433, 'GGGGC': 469649, 'GGGGG': 355549}, 'overrepresented_sequences': {}} {'total_reads': 3121402, 'total_bases': 180857750, 'q20_bases': 167270427, 'q30_bases': 156104956, 'total_cycles': 58, 'quality_curves': {'A': [37.465, 37.4702, 37.4586, 37.4793, 37.4584, 37.4633, 37.4304, 37.4239, 37.4233, 37.3868, 37.3454, 37.293, 37.2517, 37.2021, 37.1425, 37.0564, 37.0267, 36.8988, 36.8468, 36.7721, 36.7214, 36.6593, 36.6345, 36.3727, 36.1954, 35.9179, 35.7433, 35.4315, 35.1583, 34.9513, 34.8258, 34.6698, 34.536, 34.5655, 33.9132, 33.8598, 33.5841, 33.2023, 33.1256, 33.0224, 32.5751, 32.3013, 32.2311, 31.7075, 31.4369, 31.1084, 30.7138, 30.4361, 30.3266, 29.5797, 29.1733, 28.967, 28.3444, 27.9572, 27.4396, 26.5883, 26.2977, 25.9489], 'T': [37.659, 37.6255, 37.6346, 37.6231, 37.5738, 37.569, 37.5248, 37.5059, 37.4828, 37.4617, 37.4233, 37.3652, 37.3736, 37.3285, 37.3172, 37.3015, 37.2651, 37.1225, 37.0375, 36.9776, 36.9486, 36.8582, 36.8855, 36.7089, 36.5528, 36.3128, 36.1314, 35.8556, 35.6321, 35.4288, 35.3377, 35.1537, 35.03, 35.0011, 34.5072, 34.364, 34.1226, 33.8281, 33.6646, 33.556, 33.1909, 32.9024, 32.8234, 32.3829, 32.0243, 31.8435, 31.4041, 31.1291, 30.9506, 30.4029, 29.8492, 29.5929, 29.0708, 28.5887, 28.1114, 27.4283, 26.8842, 26.5954], 'C': [37.6443, 37.6615, 37.625, 37.6171, 37.6245, 37.608, 37.6122, 37.596, 37.5687, 37.5732, 37.5429, 37.5278, 37.4848, 37.4905, 37.4254, 37.368, 37.384, 37.3312, 37.2539, 37.1687, 37.1039, 37.0863, 37.031, 36.8605, 36.6676, 36.5273, 36.3546, 36.1037, 35.9244, 35.6814, 35.568, 35.4649, 35.311, 35.3076, 34.8951, 34.784, 34.4979, 34.2084, 34.1156, 33.9719, 33.5907, 33.3391, 33.1871, 32.7734, 32.5416, 32.1166, 31.7953, 31.5177, 31.2304, 30.5745, 30.339, 29.8851, 29.2906, 28.9284, 28.3092, 27.5492, 27.1542, 26.5119], 'G': [37.5361, 37.5353, 37.501, 37.5186, 37.4974, 37.482, 37.4747, 37.469, 37.4452, 37.4405, 37.4216, 37.3668, 37.3184, 37.3082, 37.2215, 37.1557, 37.1595, 37.098, 37.0421, 36.9581, 36.899, 36.821, 36.7941, 36.6121, 36.4521, 36.2804, 36.1196, 35.8977, 35.6781, 35.4636, 35.3616, 35.2206, 35.1008, 35.0823, 34.6632, 34.5294, 34.2418, 33.9479, 33.8481, 33.7275, 33.2862, 33.1249, 32.9137, 32.5222, 32.2238, 31.8131, 31.3778, 31.0881, 30.8435, 30.2395, 29.8414, 29.471, 28.8207, 28.3925, 27.8198, 27.1317, 26.7023, 26.1185], 'mean': [37.5798, 37.5772, 37.5565, 37.5606, 37.5415, 37.5318, 37.5141, 37.5035, 37.4827, 37.4708, 37.4404, 37.3954, 37.3626, 37.3425, 37.283, 37.2262, 37.219, 37.1272, 37.0604, 36.9849, 36.931, 36.8698, 36.8495, 36.6543, 36.4844, 36.2873, 36.1124, 35.8542, 35.6366, 35.4142, 35.3088, 35.1681, 35.0304, 35.0247, 34.5484, 34.4289, 34.1568, 33.8503, 33.7371, 33.6186, 33.213, 32.971, 32.836, 32.4071, 32.114, 31.7651, 31.3755, 31.0888, 30.8761, 30.2428, 29.8532, 29.5171, 28.9184, 28.502, 27.9487, 27.2105, 26.7906, 26.3013]}, 'content_curves': {'A': [0.202651, 0.19566, 0.202423, 0.203347, 0.197311, 0.203285, 0.201273, 0.195454, 0.204057, 0.198058, 0.200239, 0.204498, 0.203401, 0.197009, 0.20494, 0.202525, 0.198145, 0.20378, 0.200719, 0.195752, 0.201203, 0.199713, 0.195867, 0.200838, 0.196966, 0.19502, 0.200042, 0.195556, 0.194031, 0.198682, 0.195845, 0.194168, 0.199907, 0.196906, 0.193971, 0.200275, 0.197748, 0.194374, 0.2019, 0.197071, 0.195565, 0.199157, 0.196813, 0.192381, 0.197943, 0.198386, 0.192282, 0.199103, 0.193822, 0.193785, 0.196712, 0.194389, 0.193223, 0.197852, 0.197144, 0.193895, 0.197558, 0.19463], 'T': [0.246229, 0.229339, 0.234815, 0.228742, 0.222914, 0.229483, 0.226614, 0.224689, 0.229132, 0.2325, 0.222633, 0.228114, 0.230235, 0.219911, 0.223826, 0.224805, 0.218655, 0.224431, 0.227249, 0.222515, 0.224892, 0.226344, 0.221623, 0.223311, 0.224291, 0.221047, 0.224528, 0.226263, 0.221382, 0.225111, 0.226237, 0.220587, 0.22597, 0.2281, 0.224598, 0.225796, 0.226303, 0.221707, 0.220568, 0.22473, 0.218982, 0.223925, 0.225295, 0.22112, 0.22277, 0.226023, 0.22068, 0.223992, 0.224738, 0.22123, 0.218964, 0.224458, 0.215611, 0.217265, 0.220412, 0.213684, 0.217678, 0.215705], 'C': [0.257822, 0.272169, 0.263847, 0.264514, 0.273893, 0.266878, 0.268807, 0.276266, 0.270346, 0.271488, 0.277941, 0.273559, 0.27105, 0.278317, 0.2763, 0.27245, 0.279664, 0.275842, 0.276229, 0.27946, 0.276347, 0.273937, 0.280409, 0.276345, 0.279465, 0.285366, 0.278535, 0.277573, 0.282248, 0.276432, 0.278847, 0.28348, 0.278374, 0.278331, 0.283234, 0.278865, 0.281264, 0.283664, 0.281786, 0.280871, 0.284826, 0.279909, 0.28165, 0.28841, 0.284543, 0.279996, 0.286464, 0.282503, 0.280998, 0.283414, 0.284504, 0.281947, 0.289035, 0.285581, 0.285231, 0.289435, 0.284683, 0.287174], 'G': [0.293297, 0.302822, 0.298915, 0.303396, 0.305882, 0.300354, 0.303305, 0.303588, 0.296465, 0.297954, 0.299187, 0.29383, 0.295313, 0.304762, 0.294934, 0.300219, 0.303537, 0.295948, 0.295802, 0.302273, 0.297558, 0.300006, 0.3021, 0.299505, 0.299278, 0.298566, 0.296895, 0.300607, 0.302339, 0.299776, 0.299071, 0.301765, 0.295749, 0.296663, 0.298198, 0.295064, 0.294685, 0.300255, 0.295745, 0.297327, 0.300627, 0.297008, 0.296242, 0.29809, 0.294742, 0.295594, 0.300574, 0.294403, 0.300442, 0.301571, 0.29982, 0.299206, 0.302131, 0.299302, 0.297213, 0.302987, 0.300081, 0.302489], 'N': [0, 1.12129e-05, 0, 0, 0, 0, 0, 2.24258e-06, 0, 0, 0, 0, 0, 9.61107e-07, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1.28283e-06, 6.41514e-07, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1.93874e-06], 'GC': [0.551119, 0.57499, 0.562762, 0.56791, 0.579775, 0.567232, 0.572113, 0.579855, 0.566811, 0.569442, 0.577128, 0.567389, 0.566364, 0.583079, 0.571234, 0.57267, 0.5832, 0.57179, 0.572031, 0.581733, 0.573905, 0.573943, 0.58251, 0.575851, 0.578743, 0.583932, 0.575429, 0.57818, 0.584587, 0.576207, 0.577918, 0.585245, 0.574123, 0.574994, 0.581432, 0.573929, 0.575949, 0.583919, 0.577532, 0.578199, 0.585453, 0.576917, 0.577892, 0.5865, 0.579285, 0.575591, 0.587038, 0.576906, 0.581441, 0.584985, 0.584324, 0.581153, 0.591166, 0.584883, 0.582444, 0.592422, 0.584764, 0.589663]}, 'kmer_count': {'AAAAA': 303990, 'AAAAT': 161161, 'AAAAC': 125091, 'AAAAG': 160335, 'AAATA': 134366, 'AAATT': 111190, 'AAATC': 102589, 'AAATG': 120237, 'AAACA': 149270, 'AAACT': 134971, 'AAACC': 113657, 'AAACG': 45216, 'AAAGA': 142483, 'AAAGT': 123671, 'AAAGC': 145130, 'AAAGG': 163817, 'AATAA': 107441, 'AATAT': 66810, 'AATAC': 56904, 'AATAG': 60565, 'AATTA': 60687, 'AATTT': 113099, 'AATTC': 92412, 'AATTG': 63008, 'AATCA': 83213, 'AATCT': 126172, 'AATCC': 100753, 'AATCG': 36918, 'AATGA': 102128, 'AATGT': 100231, 'AATGC': 98000, 'AATGG': 140385, 'AACAA': 105699, 'AACAT': 100484, 'AACAC': 118295, 'AACAG': 143608, 'AACTA': 36811, 'AACTT': 156448, 'AACTC': 135125, 'AACTG': 145746, 'AACCA': 101170, 'AACCT': 98083, 'AACCC': 111058, 'AACCG': 41658, 'AACGA': 23891, 'AACGT': 32438, 'AACGC': 40180, 'AACGG': 54558, 'AAGAA': 161328, 'AAGAT': 121817, 'AAGAC': 100024, 'AAGAG': 170034, 'AAGTA': 80926, 'AAGTT': 119533, 'AAGTC': 133115, 'AAGTG': 145703, 'AAGCA': 129844, 'AAGCT': 138566, 'AAGCC': 200319, 'AAGCG': 95937, 'AAGGA': 175008, 'AAGGT': 143857, 'AAGGC': 232206, 'AAGGG': 229564, 'ATAAA': 117203, 'ATAAT': 54556, 'ATAAC': 39601, 'ATAAG': 55303, 'ATATA': 50392, 'ATATT': 65277, 'ATATC': 42842, 'ATATG': 44084, 'ATACA': 80302, 'ATACT': 75506, 'ATACC': 45109, 'ATACG': 17518, 'ATAGA': 67389, 'ATAGT': 63807, 'ATAGC': 75172, 'ATAGG': 78974, 'ATTAA': 58161, 'ATTAT': 40907, 'ATTAC': 51014, 'ATTAG': 38083, 'ATTTA': 58665, 'ATTTT': 138701, 'ATTTC': 120604, 'ATTTG': 116561, 'ATTCA': 99640, 'ATTCT': 123241, 'ATTCC': 107930, 'ATTCG': 37533, 'ATTGA': 76592, 'ATTGT': 63757, 'ATTGC': 71444, 'ATTGG': 92731, 'ATCAA': 80651, 'ATCAT': 144728, 'ATCAC': 106902, 'ATCAG': 151380, 'ATCTA': 35228, 'ATCTT': 260356, 'ATCTC': 201482, 'ATCTG': 199560, 'ATCCA': 166535, 'ATCCT': 156156, 'ATCCC': 111911, 'ATCCG': 70300, 'ATCGA': 32169, 'ATCGT': 45634, 'ATCGC': 46099, 'ATCGG': 48605, 'ATGAA': 149050, 'ATGAT': 153488, 'ATGAC': 111066, 'ATGAG': 184149, 'ATGTA': 91268, 'ATGTT': 173290, 'ATGTC': 167496, 'ATGTG': 117497, 'ATGCA': 95519, 'ATGCT': 118534, 'ATGCC': 165246, 'ATGCG': 105901, 'ATGGA': 142833, 'ATGGT': 183795, 'ATGGC': 241007, 'ATGGG': 199100, 'ACAAA': 164024, 'ACAAT': 94719, 'ACAAC': 71991, 'ACAAG': 112624, 'ACATA': 73865, 'ACATT': 113141, 'ACATC': 127214, 'ACATG': 134631, 'ACACA': 170347, 'ACACT': 101775, 'ACACC': 161909, 'ACACG': 95267, 'ACAGA': 163856, 'ACAGT': 116953, 'ACAGC': 244395, 'ACAGG': 253389, 'ACTAA': 36269, 'ACTAT': 33286, 'ACTAC': 29530, 'ACTAG': 34537, 'ACTTA': 35340, 'ACTTT': 127843, 'ACTTC': 183480, 'ACTTG': 204089, 'ACTCA': 119302, 'ACTCT': 114801, 'ACTCC': 170988, 'ACTCG': 114218, 'ACTGA': 115517, 'ACTGT': 127767, 'ACTGC': 182470, 'ACTGG': 221069, 'ACCAA': 82943, 'ACCAT': 124698, 'ACCAC': 216577, 'ACCAG': 271426, 'ACCTA': 29453, 'ACCTT': 162783, 'ACCTC': 205333, 'ACCTG': 206218, 'ACCCA': 162118, 'ACCCT': 134395, 'ACCCC': 159493, 'ACCCG': 92556, 'ACCGA': 56591, 'ACCGT': 60854, 'ACCGC': 78917, 'ACCGG': 72513, 'ACGAA': 52972, 'ACGAT': 57420, 'ACGAC': 50312, 'ACGAG': 69019, 'ACGTA': 34613, 'ACGTT': 77916, 'ACGTC': 94616, 'ACGTG': 86805, 'ACGCA': 64611, 'ACGCT': 72811, 'ACGCC': 117193, 'ACGCG': 54002, 'ACGGA': 79845, 'ACGGT': 89703, 'ACGGC': 122537, 'ACGGG': 122370, 'AGAAA': 182905, 'AGAAT': 93851, 'AGAAC': 138492, 'AGAAG': 262237, 'AGATA': 77476, 'AGATT': 71324, 'AGATC': 113099, 'AGATG': 213757, 'AGACA': 133004, 'AGACT': 89471, 'AGACC': 149589, 'AGACG': 95319, 'AGAGA': 187360, 'AGAGT': 104706, 'AGAGC': 186397, 'AGAGG': 309637, 'AGTAA': 67491, 'AGTAT': 51539, 'AGTAC': 67079, 'AGTAG': 97828, 'AGTTA': 52281, 'AGTTT': 128224, 'AGTTC': 131446, 'AGTTG': 134554, 'AGTCA': 123524, 'AGTCT': 131763, 'AGTCC': 188255, 'AGTCG': 82414, 'AGTGA': 141453, 'AGTGT': 107175, 'AGTGC': 145958, 'AGTGG': 216244, 'AGCAA': 110971, 'AGCAT': 130876, 'AGCAC': 210424, 'AGCAG': 371999, 'AGCTA': 55941, 'AGCTT': 224519, 'AGCTC': 283384, 'AGCTG': 370222, 'AGCCA': 274433, 'AGCCT': 228185, 'AGCCC': 302140, 'AGCCG': 190032, 'AGCGA': 79690, 'AGCGT': 118391, 'AGCGC': 158823, 'AGCGG': 194792, 'AGGAA': 282540, 'AGGAT': 186983, 'AGGAC': 206064, 'AGGAG': 368247, 'AGGTA': 128373, 'AGGTT': 149692, 'AGGTC': 234883, 'AGGTG': 324869, 'AGGCA': 278250, 'AGGCT': 296012, 'AGGCC': 367138, 'AGGCG': 229612, 'AGGGA': 262563, 'AGGGT': 224953, 'AGGGC': 401675, 'AGGGG': 449412, 'TAAAA': 133163, 'TAAAT': 79952, 'TAAAC': 56441, 'TAAAG': 64727, 'TAATA': 43911, 'TAATT': 62259, 'TAATC': 56861, 'TAATG': 61279, 'TAACA': 61383, 'TAACT': 59349, 'TAACC': 38913, 'TAACG': 11447, 'TAAGA': 57468, 'TAAGT': 39737, 'TAAGC': 49140, 'TAAGG': 72440, 'TATAA': 41989, 'TATAT': 42752, 'TATAC': 28214, 'TATAG': 38887, 'TATTA': 33166, 'TATTT': 110644, 'TATTC': 62053, 'TATTG': 65423, 'TATCA': 52794, 'TATCT': 62581, 'TATCC': 53833, 'TATCG': 20111, 'TATGA': 44303, 'TATGT': 42883, 'TATGC': 31795, 'TATGG': 66019, 'TACAA': 74618, 'TACAT': 70603, 'TACAC': 72137, 'TACAG': 109732, 'TACTA': 20133, 'TACTT': 110611, 'TACTC': 80743, 'TACTG': 86754, 'TACCA': 62001, 'TACCT': 50366, 'TACCC': 55277, 'TACCG': 30142, 'TACGA': 18286, 'TACGT': 23516, 'TACGC': 19347, 'TACGG': 39931, 'TAGAA': 88797, 'TAGAT': 68845, 'TAGAC': 52066, 'TAGAG': 93584, 'TAGTA': 54493, 'TAGTT': 64261, 'TAGTC': 70272, 'TAGTG': 75820, 'TAGCA': 68390, 'TAGCT': 77298, 'TAGCC': 104372, 'TAGCG': 61437, 'TAGGA': 101884, 'TAGGT': 88816, 'TAGGC': 97298, 'TAGGG': 120368, 'TTAAA': 105730, 'TTAAT': 70980, 'TTAAC': 47950, 'TTAAG': 61189, 'TTATA': 42966, 'TTATT': 78823, 'TTATC': 52327, 'TTATG': 48420, 'TTACA': 79121, 'TTACT': 44484, 'TTACC': 39577, 'TTACG': 25315, 'TTAGA': 35795, 'TTAGT': 48213, 'TTAGC': 48131, 'TTAGG': 68352, 'TTTAA': 104658, 'TTTAT': 91124, 'TTTAC': 60472, 'TTTAG': 57511, 'TTTTA': 101911, 'TTTTT': 307969, 'TTTTC': 201715, 'TTTTG': 155959, 'TTTCA': 148303, 'TTTCT': 288733, 'TTTCC': 193494, 'TTTCG': 37459, 'TTTGA': 125707, 'TTTGT': 176044, 'TTTGC': 142499, 'TTTGG': 174767, 'TTCAA': 136792, 'TTCAT': 182086, 'TTCAC': 211449, 'TTCAG': 250764, 'TTCTA': 66738, 'TTCTT': 428060, 'TTCTC': 377081, 'TTCTG': 288558, 'TTCCA': 201008, 'TTCCT': 263782, 'TTCCC': 214012, 'TTCCG': 125749, 'TTCGA': 46288, 'TTCGT': 36920, 'TTCGC': 39153, 'TTCGG': 65925, 'TTGAA': 170615, 'TTGAT': 187608, 'TTGAC': 139613, 'TTGAG': 202160, 'TTGTA': 140493, 'TTGTT': 188535, 'TTGTC': 214783, 'TTGTG': 151927, 'TTGCA': 133036, 'TTGCT': 176645, 'TTGCC': 193306, 'TTGCG': 123777, 'TTGGA': 194550, 'TTGGT': 215138, 'TTGGC': 273689, 'TTGGG': 268609, 'TCAAA': 160284, 'TCAAT': 125294, 'TCAAC': 72439, 'TCAAG': 109756, 'TCATA': 91936, 'TCATT': 110009, 'TCATC': 285148, 'TCATG': 195119, 'TCACA': 186940, 'TCACT': 170398, 'TCACC': 207328, 'TCACG': 102250, 'TCAGA': 150239, 'TCAGT': 143821, 'TCAGC': 304520, 'TCAGG': 308214, 'TCTAA': 44414, 'TCTAT': 52125, 'TCTAC': 58872, 'TCTAG': 60990, 'TCTTA': 69388, 'TCTTT': 224680, 'TCTTC': 458723, 'TCTTG': 427791, 'TCTCA': 165861, 'TCTCT': 252729, 'TCTCC': 429589, 'TCTCG': 186645, 'TCTGA': 163914, 'TCTGT': 191353, 'TCTGC': 294867, 'TCTGG': 397248, 'TCCAA': 140015, 'TCCAT': 184947, 'TCCAC': 328229, 'TCCAG': 499958, 'TCCTA': 55833, 'TCCTT': 334032, 'TCCTC': 556873, 'TCCTG': 345356, 'TCCCA': 247516, 'TCCCT': 176286, 'TCCCC': 267335, 'TCCCG': 213029, 'TCCGA': 79409, 'TCCGT': 112929, 'TCCGC': 158331, 'TCCGG': 194611, 'TCGAA': 98782, 'TCGAT': 89957, 'TCGAC': 37931, 'TCGAG': 58171, 'TCGTA': 76809, 'TCGTT': 69022, 'TCGTC': 141422, 'TCGTG': 96172, 'TCGCA': 76892, 'TCGCT': 98229, 'TCGCC': 118814, 'TCGCG': 65922, 'TCGGA': 85258, 'TCGGT': 86664, 'TCGGC': 172192, 'TCGGG': 186039, 'TGAAA': 116871, 'TGAAT': 93873, 'TGAAC': 134040, 'TGAAG': 286614, 'TGATA': 58394, 'TGATT': 64764, 'TGATC': 151168, 'TGATG': 293544, 'TGACA': 139130, 'TGACT': 105527, 'TGACC': 172183, 'TGACG': 130669, 'TGAGA': 170199, 'TGAGT': 120051, 'TGAGC': 246683, 'TGAGG': 337562, 'TGTAA': 77513, 'TGTAT': 64272, 'TGTAC': 100790, 'TGTAG': 188260, 'TGTTA': 64741, 'TGTTT': 155503, 'TGTTC': 178671, 'TGTTG': 254072, 'TGTCA': 173326, 'TGTCT': 199931, 'TGTCC': 297605, 'TGTCG': 118031, 'TGTGA': 129429, 'TGTGT': 158388, 'TGTGC': 170564, 'TGTGG': 275243, 'TGCAA': 106330, 'TGCAT': 131002, 'TGCAC': 174677, 'TGCAG': 342515, 'TGCTA': 47818, 'TGCTT': 204453, 'TGCTC': 267373, 'TGCTG': 421750, 'TGCCA': 234039, 'TGCCT': 220370, 'TGCCC': 294907, 'TGCCG': 206802, 'TGCGA': 49425, 'TGCGT': 79366, 'TGCGC': 139319, 'TGCGG': 237088, 'TGGAA': 227577, 'TGGAT': 161129, 'TGGAC': 183682, 'TGGAG': 367855, 'TGGTA': 115630, 'TGGTT': 172549, 'TGGTC': 292084, 'TGGTG': 360142, 'TGGCA': 283701, 'TGGCT': 308817, 'TGGCC': 444397, 'TGGCG': 231310, 'TGGGA': 273006, 'TGGGT': 266904, 'TGGGC': 402893, 'TGGGG': 573860, 'CAAAA': 173173, 'CAAAT': 102996, 'CAAAC': 154350, 'CAAAG': 198494, 'CAATA': 56076, 'CAATT': 68935, 'CAATC': 91841, 'CAATG': 151869, 'CAACA': 116474, 'CAACT': 86771, 'CAACC': 89348, 'CAACG': 45088, 'CAAGA': 107400, 'CAAGT': 112268, 'CAAGC': 108445, 'CAAGG': 188870, 'CATAA': 64442, 'CATAT': 48799, 'CATAC': 84183, 'CATAG': 125732, 'CATTA': 59549, 'CATTT': 116176, 'CATTC': 138294, 'CATTG': 119610, 'CATCA': 212963, 'CATCT': 266562, 'CATCC': 228506, 'CATCG': 81732, 'CATGA': 192282, 'CATGT': 167077, 'CATGC': 158856, 'CATGG': 255699, 'CACAA': 166720, 'CACAT': 168017, 'CACAC': 205123, 'CACAG': 312581, 'CACTA': 45729, 'CACTT': 152185, 'CACTC': 164052, 'CACTG': 290081, 'CACCA': 343789, 'CACCT': 297798, 'CACCC': 221277, 'CACCG': 118948, 'CACGA': 109155, 'CACGT': 148014, 'CACGC': 153442, 'CACGG': 190420, 'CAGAA': 219054, 'CAGAT': 154161, 'CAGAC': 163131, 'CAGAG': 287975, 'CAGTA': 85988, 'CAGTT': 151588, 'CAGTC': 164599, 'CAGTG': 235811, 'CAGCA': 427506, 'CAGCT': 466127, 'CAGCC': 431819, 'CAGCG': 254190, 'CAGGA': 430020, 'CAGGT': 359439, 'CAGGC': 470958, 'CAGGG': 577014, 'CTAAA': 49043, 'CTAAT': 32017, 'CTAAC': 38204, 'CTAAG': 49371, 'CTATA': 22202, 'CTATT': 51159, 'CTATC': 43001, 'CTATG': 42565, 'CTACA': 55244, 'CTACT': 47633, 'CTACC': 46015, 'CTACG': 24525, 'CTAGA': 56884, 'CTAGT': 23219, 'CTAGC': 45758, 'CTAGG': 77081, 'CTTAA': 67629, 'CTTAT': 42932, 'CTTAC': 33565, 'CTTAG': 63046, 'CTTTA': 103652, 'CTTTT': 177932, 'CTTTC': 181410, 'CTTTG': 207427, 'CTTCA': 363440, 'CTTCT': 511717, 'CTTCC': 354412, 'CTTCG': 70999, 'CTTGA': 305870, 'CTTGT': 275420, 'CTTGC': 282474, 'CTTGG': 433709, 'CTCAA': 139531, 'CTCAT': 208422, 'CTCAC': 156248, 'CTCAG': 299884, 'CTCTA': 67386, 'CTCTT': 270317, 'CTCTC': 233526, 'CTCTG': 335134, 'CTCCA': 494944, 'CTCCT': 561190, 'CTCCC': 305052, 'CTCCG': 219481, 'CTCGA': 126946, 'CTCGT': 186249, 'CTCGC': 182013, 'CTCGG': 261158, 'CTGAA': 156835, 'CTGAT': 101044, 'CTGAC': 149916, 'CTGAG': 269433, 'CTGTA': 113473, 'CTGTT': 160675, 'CTGTC': 241521, 'CTGTG': 275526, 'CTGCA': 349150, 'CTGCT': 412520, 'CTGCC': 388301, 'CTGCG': 165898, 'CTGGA': 343747, 'CTGGT': 323897, 'CTGGC': 404579, 'CTGGG': 629636, 'CCAAA': 175302, 'CCAAT': 71585, 'CCAAC': 110473, 'CCAAG': 169811, 'CCATA': 86505, 'CCATT': 123182, 'CCATC': 209780, 'CCATG': 269472, 'CCACA': 293436, 'CCACT': 201311, 'CCACC': 399670, 'CCACG': 254182, 'CCAGA': 265808, 'CCAGT': 201291, 'CCAGC': 542794, 'CCAGG': 689572, 'CCTAA': 39595, 'CCTAT': 43538, 'CCTAC': 35895, 'CCTAG': 54880, 'CCTTA': 49024, 'CCTTT': 154584, 'CCTTC': 344816, 'CCTTG': 386462, 'CCTCA': 280014, 'CCTCT': 302220, 'CCTCC': 567550, 'CCTCG': 274891, 'CCTGA': 182111, 'CCTGT': 189683, 'CCTGC': 355295, 'CCTGG': 502411, 'CCCAA': 152725, 'CCCAT': 184117, 'CCCAC': 283856, 'CCCAG': 489016, 'CCCTA': 46928, 'CCCTT': 205603, 'CCCTC': 279015, 'CCCTG': 311413, 'CCCCA': 338816, 'CCCCT': 228247, 'CCCCC': 287290, 'CCCCG': 250299, 'CCCGA': 123549, 'CCCGT': 136029, 'CCCGC': 224181, 'CCCGG': 322556, 'CCGAA': 76769, 'CCGAT': 71073, 'CCGAC': 76369, 'CCGAG': 156360, 'CCGTA': 64833, 'CCGTT': 91969, 'CCGTC': 158995, 'CCGTG': 163925, 'CCGCA': 153426, 'CCGCT': 205609, 'CCGCC': 278631, 'CCGCG': 145310, 'CCGGA': 148020, 'CCGGT': 148825, 'CCGGC': 268563, 'CCGGG': 328376, 'CGAAA': 41974, 'CGAAT': 38746, 'CGAAC': 59720, 'CGAAG': 140577, 'CGATA': 19336, 'CGATT': 29137, 'CGATC': 76073, 'CGATG': 167107, 'CGACA': 52685, 'CGACT': 59092, 'CGACC': 69773, 'CGACG': 45199, 'CGAGA': 78333, 'CGAGT': 61371, 'CGAGC': 95962, 'CGAGG': 169986, 'CGTAA': 19726, 'CGTAT': 23583, 'CGTAC': 50159, 'CGTAG': 126554, 'CGTTA': 17572, 'CGTTT': 68269, 'CGTTC': 108001, 'CGTTG': 124552, 'CGTCA': 96787, 'CGTCT': 135569, 'CGTCC': 183032, 'CGTCG': 118824, 'CGTGA': 88448, 'CGTGT': 92430, 'CGTGC': 128024, 'CGTGG': 205644, 'CGCAA': 36460, 'CGCAT': 60376, 'CGCAC': 112405, 'CGCAG': 238616, 'CGCTA': 17255, 'CGCTT': 116605, 'CGCTC': 189874, 'CGCTG': 221018, 'CGCCA': 159884, 'CGCCT': 160450, 'CGCCC': 229871, 'CGCCG': 182287, 'CGCGA': 51463, 'CGCGT': 58740, 'CGCGC': 112066, 'CGCGG': 174987, 'CGGAA': 96854, 'CGGAT': 119525, 'CGGAC': 96023, 'CGGAG': 172937, 'CGGTA': 84765, 'CGGTT': 80657, 'CGGTC': 127108, 'CGGTG': 199592, 'CGGCA': 164874, 'CGGCT': 194667, 'CGGCC': 322826, 'CGGCG': 219224, 'CGGGA': 173862, 'CGGGT': 123864, 'CGGGC': 309257, 'CGGGG': 338413, 'GAAAA': 137676, 'GAAAT': 120965, 'GAAAC': 107770, 'GAAAG': 151103, 'GAATA': 57606, 'GAATT': 86027, 'GAATC': 94294, 'GAATG': 105247, 'GAACA': 141151, 'GAACT': 193172, 'GAACC': 111591, 'GAACG': 48988, 'GAAGA': 246275, 'GAAGT': 202904, 'GAAGC': 261544, 'GAAGG': 357215, 'GATAA': 52699, 'GATAT': 44391, 'GATAC': 49184, 'GATAG': 60218, 'GATTA': 32935, 'GATTT': 93214, 'GATTC': 75730, 'GATTG': 55880, 'GATCA': 132955, 'GATCT': 236921, 'GATCC': 122446, 'GATCG': 34128, 'GATGA': 256798, 'GATGT': 236833, 'GATGC': 195287, 'GATGG': 304862, 'GACAA': 94690, 'GACAT': 110194, 'GACAC': 133782, 'GACAG': 212565, 'GACTA': 30423, 'GACTT': 131928, 'GACTC': 141525, 'GACTG': 123563, 'GACCA': 187791, 'GACCT': 160026, 'GACCC': 165263, 'GACCG': 78410, 'GACGA': 78850, 'GACGT': 90373, 'GACGC': 96212, 'GACGG': 130706, 'GAGAA': 207864, 'GAGAT': 130492, 'GAGAC': 153347, 'GAGAG': 236008, 'GAGTA': 61025, 'GAGTT': 110958, 'GAGTC': 156680, 'GAGTG': 151043, 'GAGCA': 198812, 'GAGCT': 254050, 'GAGCC': 262410, 'GAGCG': 140913, 'GAGGA': 333610, 'GAGGT': 242663, 'GAGGC': 372145, 'GAGGG': 410570, 'GTAAA': 62090, 'GTAAT': 66383, 'GTAAC': 44504, 'GTAAG': 52656, 'GTATA': 35451, 'GTATT': 72470, 'GTATC': 49963, 'GTATG': 49655, 'GTACA': 111864, 'GTACT': 130392, 'GTACC': 67314, 'GTACG': 34388, 'GTAGA': 143539, 'GTAGT': 128643, 'GTAGC': 141674, 'GTAGG': 182776, 'GTTAA': 54267, 'GTTAT': 45535, 'GTTAC': 43281, 'GTTAG': 39981, 'GTTTA': 47406, 'GTTTT': 141661, 'GTTTC': 162757, 'GTTTG': 137130, 'GTTCA': 167884, 'GTTCT': 232828, 'GTTCC': 149408, 'GTTCG': 43178, 'GTTGA': 188426, 'GTTGT': 177837, 'GTTGC': 128898, 'GTTGG': 249529, 'GTCAA': 109856, 'GTCAT': 144918, 'GTCAC': 190349, 'GTCAG': 202562, 'GTCTA': 45993, 'GTCTT': 218802, 'GTCTC': 221193, 'GTCTG': 221730, 'GTCCA': 288354, 'GTCCT': 307759, 'GTCCC': 275570, 'GTCCG': 130164, 'GTCGA': 79382, 'GTCGT': 113877, 'GTCGC': 92893, 'GTCGG': 154987, 'GTGAA': 153028, 'GTGAT': 122333, 'GTGAC': 145792, 'GTGAG': 216910, 'GTGTA': 83553, 'GTGTT': 128526, 'GTGTC': 161393, 'GTGTG': 183891, 'GTGCA': 176223, 'GTGCT': 232310, 'GTGCC': 211433, 'GTGCG': 108040, 'GTGGA': 256800, 'GTGGT': 214802, 'GTGGC': 348787, 'GTGGG': 419057, 'GCAAA': 130847, 'GCAAT': 77654, 'GCAAC': 84029, 'GCAAG': 123994, 'GCATA': 72846, 'GCATT': 87704, 'GCATC': 168451, 'GCATG': 173496, 'GCACA': 202471, 'GCACT': 178333, 'GCACC': 216099, 'GCACG': 150406, 'GCAGA': 244338, 'GCAGT': 175328, 'GCAGC': 494062, 'GCAGG': 587563, 'GCTAA': 48351, 'GCTAT': 29970, 'GCTAC': 49667, 'GCTAG': 52701, 'GCTTA': 53850, 'GCTTT': 161947, 'GCTTC': 316042, 'GCTTG': 277568, 'GCTCA': 238053, 'GCTCT': 237418, 'GCTCC': 416702, 'GCTCG': 182602, 'GCTGA': 216424, 'GCTGT': 281007, 'GCTGC': 485298, 'GCTGG': 583034, 'GCCAA': 153417, 'GCCAT': 194957, 'GCCAC': 322484, 'GCCAG': 439359, 'GCCTA': 42321, 'GCCTT': 235011, 'GCCTC': 381548, 'GCCTG': 366780, 'GCCCA': 366638, 'GCCCT': 307617, 'GCCCC': 401193, 'GCCCG': 255875, 'GCCGA': 122671, 'GCCGT': 169929, 'GCCGC': 323699, 'GCCGG': 307274, 'GCGAA': 52984, 'GCGAT': 72917, 'GCGAC': 63721, 'GCGAG': 123074, 'GCGTA': 43553, 'GCGTT': 78223, 'GCGTC': 141087, 'GCGTG': 167668, 'GCGCA': 152786, 'GCGCT': 169928, 'GCGCC': 221390, 'GCGCG': 133993, 'GCGGA': 172721, 'GCGGT': 169226, 'GCGGC': 342078, 'GCGGG': 312783, 'GGAAA': 174817, 'GGAAT': 117096, 'GGAAC': 163070, 'GGAAG': 379516, 'GGATA': 51081, 'GGATT': 90653, 'GGATC': 184903, 'GGATG': 318374, 'GGACA': 223702, 'GGACT': 174942, 'GGACC': 201004, 'GGACG': 124653, 'GGAGA': 292182, 'GGAGT': 193273, 'GGAGC': 328156, 'GGAGG': 540889, 'GGTAA': 60563, 'GGTAT': 66659, 'GGTAC': 125530, 'GGTAG': 184168, 'GGTTA': 47554, 'GGTTT': 136895, 'GGTTC': 176987, 'GGTTG': 229657, 'GGTCA': 251580, 'GGTCT': 240883, 'GGTCC': 331015, 'GGTCG': 120727, 'GGTGA': 276240, 'GGTGT': 195501, 'GGTGC': 281845, 'GGTGG': 539086, 'GGCAA': 162789, 'GGCAT': 179548, 'GGCAC': 252387, 'GGCAG': 551149, 'GGCTA': 60090, 'GGCTT': 266332, 'GGCTC': 335379, 'GGCTG': 548410, 'GGCCA': 446923, 'GGCCT': 419159, 'GGCCC': 509374, 'GGCCG': 349473, 'GGCGA': 131792, 'GGCGT': 174502, 'GGCGC': 267872, 'GGCGG': 391985, 'GGGAA': 228558, 'GGGAT': 174688, 'GGGAC': 239391, 'GGGAG': 446365, 'GGGTA': 108303, 'GGGTT': 187378, 'GGGTC': 292310, 'GGGTG': 399772, 'GGGCA': 417928, 'GGGCT': 411437, 'GGGCC': 597488, 'GGGCG': 287225, 'GGGGA': 378769, 'GGGGT': 371930, 'GGGGC': 604275, 'GGGGG': 567547}, 'overrepresented_sequences': {}} {'total_reads': 3309009, 'total_bases': 208445695, 'q20_bases': 183631939, 'q30_bases': 169926963, 'total_cycles': 63, 'quality_curves': {'A': [37.3485, 37.3517, 37.3351, 37.357, 37.3341, 37.3303, 37.292, 37.2846, 37.2771, 37.232, 37.1831, 37.1135, 37.0573, 36.9948, 36.9235, 36.8176, 36.7817, 36.6319, 36.575, 36.4721, 36.4141, 36.3318, 36.2983, 36.016, 35.8238, 35.5128, 35.3168, 34.9835, 34.6829, 34.4462, 34.2865, 34.0879, 33.9234, 33.9154, 33.1875, 33.0967, 32.8023, 32.3989, 32.3142, 32.1969, 31.7523, 31.473, 31.4005, 30.8733, 30.6021, 30.286, 29.8638, 29.6022, 29.4914, 28.7465, 28.3424, 28.1362, 27.5302, 27.1238, 26.6312, 25.787, 25.4995, 25.1513, 24.0652, 22.9476, 22.2173, 20.1636, 10.5104], 'T': [37.5691, 37.525, 37.5355, 37.5188, 37.4573, 37.4459, 37.3938, 37.3661, 37.332, 37.3004, 37.2529, 37.1854, 37.1907, 37.1349, 37.1187, 37.0956, 37.0519, 36.893, 36.8045, 36.7274, 36.684, 36.5829, 36.5876, 36.3633, 36.1862, 35.9047, 35.6911, 35.3813, 35.1272, 34.8988, 34.7916, 34.5678, 34.3995, 34.3483, 33.8073, 33.6123, 33.3557, 33.0302, 32.8215, 32.7227, 32.3409, 32.038, 31.9585, 31.508, 31.1485, 30.9769, 30.544, 30.2527, 30.0883, 29.5541, 28.9887, 28.7675, 28.2291, 27.7605, 27.2986, 26.6218, 26.082, 25.7978, 24.8936, 23.6152, 22.7745, 21.0063, 11.5469], 'C': [37.5373, 37.5569, 37.5138, 37.5049, 37.5097, 37.4886, 37.4849, 37.4673, 37.4365, 37.4388, 37.3957, 37.3722, 37.3197, 37.3161, 37.2391, 37.171, 37.1807, 37.1127, 37.0244, 36.9227, 36.8433, 36.8018, 36.7319, 36.5408, 36.3313, 36.1612, 35.9553, 35.662, 35.458, 35.1769, 35.0276, 34.8874, 34.6806, 34.6407, 34.1436, 33.9944, 33.6636, 33.3367, 33.2415, 33.0728, 32.6889, 32.4287, 32.2601, 31.8548, 31.6189, 31.1733, 30.8591, 30.5776, 30.2748, 29.624, 29.3816, 28.9093, 28.3297, 27.9719, 27.3498, 26.5933, 26.1927, 25.5704, 24.7293, 23.6543, 22.5515, 20.4917, 11.6043], 'G': [37.4029, 37.4002, 37.3574, 37.3797, 37.3513, 37.3289, 37.319, 37.3129, 37.2791, 37.2719, 37.2407, 37.1704, 37.1013, 37.0914, 36.9847, 36.9082, 36.9044, 36.8272, 36.7539, 36.6645, 36.5919, 36.4909, 36.4486, 36.246, 36.0665, 35.8629, 35.6801, 35.4223, 35.1671, 34.9171, 34.774, 34.5904, 34.4214, 34.3391, 33.8643, 33.6771, 33.3325, 33.0222, 32.9025, 32.7698, 32.3232, 32.1526, 31.9351, 31.5417, 31.2375, 30.8145, 30.3787, 30.091, 29.8414, 29.2369, 28.8409, 28.4519, 27.8161, 27.3875, 26.8125, 26.1539, 25.7251, 25.1438, 24.2024, 23.0976, 21.9406, 20.0962, 11.8597], 'mean': [37.4675, 37.4617, 37.4359, 37.44, 37.415, 37.3987, 37.3752, 37.3619, 37.3334, 37.316, 37.275, 37.2175, 37.1722, 37.1445, 37.0725, 37.0036, 36.9897, 36.881, 36.8044, 36.7131, 36.6464, 36.5652, 36.5294, 36.3076, 36.1197, 35.8892, 35.6869, 35.3942, 35.1469, 34.8916, 34.7536, 34.5724, 34.3895, 34.3421, 33.8, 33.6353, 33.3265, 32.9927, 32.8619, 32.732, 32.3202, 32.0697, 31.9266, 31.4963, 31.2002, 30.8473, 30.4541, 30.1678, 29.9507, 29.3219, 28.9294, 28.5904, 27.9983, 27.5829, 27.0369, 26.3097, 25.8916, 25.4083, 24.4747, 23.337, 22.3487, 20.415, 11.4966]}, 'content_curves': {'A': [0.201803, 0.194872, 0.201709, 0.202478, 0.196459, 0.202565, 0.200432, 0.194743, 0.203333, 0.197323, 0.199452, 0.20375, 0.202707, 0.196392, 0.204227, 0.201779, 0.19756, 0.203323, 0.200094, 0.195192, 0.200656, 0.199076, 0.195304, 0.200161, 0.196231, 0.194291, 0.199151, 0.194628, 0.193222, 0.197918, 0.195154, 0.193432, 0.198987, 0.196011, 0.193228, 0.199502, 0.196957, 0.19355, 0.201072, 0.196238, 0.19466, 0.198221, 0.195994, 0.191605, 0.197096, 0.197393, 0.191482, 0.198114, 0.193025, 0.192924, 0.195959, 0.19352, 0.192484, 0.197286, 0.195984, 0.193307, 0.196268, 0.19384, 0.190692, 0.196336, 0.192769, 0.189684, 0.155278], 'T': [0.246049, 0.229247, 0.234787, 0.228565, 0.222798, 0.229387, 0.226339, 0.224538, 0.229052, 0.232166, 0.222262, 0.22771, 0.229555, 0.219343, 0.223268, 0.224219, 0.218106, 0.223685, 0.226292, 0.221676, 0.224181, 0.225371, 0.220676, 0.222756, 0.223701, 0.220519, 0.2241, 0.225769, 0.220888, 0.224544, 0.225498, 0.219883, 0.225296, 0.227279, 0.223766, 0.225106, 0.225468, 0.220921, 0.220055, 0.22403, 0.218408, 0.223253, 0.224607, 0.220492, 0.222214, 0.225289, 0.219963, 0.223331, 0.223896, 0.220273, 0.218146, 0.223313, 0.214718, 0.216391, 0.219119, 0.212515, 0.217001, 0.214409, 0.211359, 0.213092, 0.215371, 0.2102, 0.168692], 'C': [0.258026, 0.272266, 0.263858, 0.264728, 0.274069, 0.267016, 0.269084, 0.276389, 0.270487, 0.271555, 0.27803, 0.273756, 0.271285, 0.278534, 0.276544, 0.272645, 0.279798, 0.275952, 0.276425, 0.279695, 0.276449, 0.274275, 0.280639, 0.276501, 0.279572, 0.285297, 0.278629, 0.277686, 0.282391, 0.276563, 0.278923, 0.283514, 0.278498, 0.278445, 0.283543, 0.279039, 0.281406, 0.283954, 0.281779, 0.281104, 0.284988, 0.280142, 0.281756, 0.288455, 0.284513, 0.280196, 0.286502, 0.282681, 0.281187, 0.283653, 0.284657, 0.282269, 0.289125, 0.285634, 0.285606, 0.289586, 0.285066, 0.287864, 0.290699, 0.286217, 0.286991, 0.289887, 0.318582], 'G': [0.294122, 0.303605, 0.299646, 0.304229, 0.306674, 0.301032, 0.304146, 0.304328, 0.297129, 0.298956, 0.300256, 0.294783, 0.296453, 0.30573, 0.295962, 0.301357, 0.304536, 0.297041, 0.297189, 0.303438, 0.298714, 0.301279, 0.303381, 0.300582, 0.300496, 0.299893, 0.29812, 0.301917, 0.303498, 0.300975, 0.300425, 0.303171, 0.29722, 0.298265, 0.299463, 0.296353, 0.29617, 0.301575, 0.297094, 0.298629, 0.301944, 0.298383, 0.297626, 0.29944, 0.296149, 0.29712, 0.302053, 0.295874, 0.301884, 0.303151, 0.301239, 0.300898, 0.303674, 0.300679, 0.299291, 0.304592, 0.301665, 0.303886, 0.307213, 0.304317, 0.304859, 0.310229, 0.355475], 'N': [0, 1.05772e-05, 0, 0, 0, 0, 0, 2.11544e-06, 0, 0, 0, 0, 0, 9.06616e-07, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1.63202e-05, 7.85791e-06, 2.84097e-05, 6.04468e-07, 0, 0, 9.36964e-06, 0, 0, 0, 0, 1.05805e-05, 0, 0, 0, 1.81416e-06, 3.68879e-05, 3.83997e-05, 9.67552e-06, 0, 0.00197199], 'GC': [0.552148, 0.575871, 0.563504, 0.568957, 0.580743, 0.568047, 0.573229, 0.580717, 0.567615, 0.570511, 0.578286, 0.56854, 0.567738, 0.584264, 0.572506, 0.574002, 0.584334, 0.572992, 0.573614, 0.583132, 0.575163, 0.575554, 0.58402, 0.577083, 0.580069, 0.585191, 0.576749, 0.579603, 0.585889, 0.577538, 0.579348, 0.586685, 0.575717, 0.57671, 0.583006, 0.575392, 0.577576, 0.585529, 0.578873, 0.579732, 0.586932, 0.578525, 0.579382, 0.587895, 0.580662, 0.577317, 0.588554, 0.578554, 0.58307, 0.586804, 0.585896, 0.583167, 0.592799, 0.586313, 0.584897, 0.594178, 0.586731, 0.59175, 0.597912, 0.590534, 0.59185, 0.600116, 0.674058]}, 'kmer_count': {'AAAAA': 379810, 'AAAAT': 183741, 'AAAAC': 146937, 'AAAAG': 187364, 'AAATA': 152843, 'AAATT': 128232, 'AAATC': 116864, 'AAATG': 135570, 'AAACA': 172258, 'AAACT': 153440, 'AAACC': 133361, 'AAACG': 52604, 'AAAGA': 164756, 'AAAGT': 141420, 'AAAGC': 167961, 'AAAGG': 192724, 'AATAA': 121798, 'AATAT': 75191, 'AATAC': 66026, 'AATAG': 71771, 'AATTA': 68578, 'AATTT': 131540, 'AATTC': 106428, 'AATTG': 71843, 'AATCA': 93378, 'AATCT': 142575, 'AATCC': 115389, 'AATCG': 44764, 'AATGA': 115070, 'AATGT': 112533, 'AATGC': 111235, 'AATGG': 159913, 'AACAA': 121535, 'AACAT': 114399, 'AACAC': 135603, 'AACAG': 166463, 'AACTA': 41907, 'AACTT': 176922, 'AACTC': 153392, 'AACTG': 167535, 'AACCA': 116981, 'AACCT': 112221, 'AACCC': 132475, 'AACCG': 50196, 'AACGA': 27630, 'AACGT': 36905, 'AACGC': 46738, 'AACGG': 63911, 'AAGAA': 185383, 'AAGAT': 137883, 'AAGAC': 117035, 'AAGAG': 195539, 'AAGTA': 91816, 'AAGTT': 139265, 'AAGTC': 152207, 'AAGTG': 164267, 'AAGCA': 148373, 'AAGCT': 157707, 'AAGCC': 230584, 'AAGCG': 112322, 'AAGGA': 200565, 'AAGGT': 166130, 'AAGGC': 271103, 'AAGGG': 276566, 'ATAAA': 133779, 'ATAAT': 62858, 'ATAAC': 45542, 'ATAAG': 62594, 'ATATA': 59916, 'ATATT': 73383, 'ATATC': 48652, 'ATATG': 49577, 'ATACA': 91416, 'ATACT': 86474, 'ATACC': 53762, 'ATACG': 20460, 'ATAGA': 77036, 'ATAGT': 71495, 'ATAGC': 86868, 'ATAGG': 92907, 'ATTAA': 66916, 'ATTAT': 46067, 'ATTAC': 56920, 'ATTAG': 43616, 'ATTTA': 67235, 'ATTTT': 162953, 'ATTTC': 138211, 'ATTTG': 131838, 'ATTCA': 112234, 'ATTCT': 140741, 'ATTCC': 124701, 'ATTCG': 43708, 'ATTGA': 86536, 'ATTGT': 71917, 'ATTGC': 82159, 'ATTGG': 107291, 'ATCAA': 91895, 'ATCAT': 163593, 'ATCAC': 120846, 'ATCAG': 169784, 'ATCTA': 39389, 'ATCTT': 291691, 'ATCTC': 228682, 'ATCTG': 224782, 'ATCCA': 187594, 'ATCCT': 177255, 'ATCCC': 135247, 'ATCCG': 81558, 'ATCGA': 37010, 'ATCGT': 52206, 'ATCGC': 54581, 'ATCGG': 58588, 'ATGAA': 167949, 'ATGAT': 172185, 'ATGAC': 126940, 'ATGAG': 207863, 'ATGTA': 102052, 'ATGTT': 195687, 'ATGTC': 190186, 'ATGTG': 133649, 'ATGCA': 109996, 'ATGCT': 133965, 'ATGCC': 190305, 'ATGCG': 122323, 'ATGGA': 162476, 'ATGGT': 205967, 'ATGGC': 280758, 'ATGGG': 235050, 'ACAAA': 188335, 'ACAAT': 105176, 'ACAAC': 82735, 'ACAAG': 128866, 'ACATA': 84897, 'ACATT': 128498, 'ACATC': 144334, 'ACATG': 155485, 'ACACA': 194015, 'ACACT': 114865, 'ACACC': 187019, 'ACACG': 110773, 'ACAGA': 188820, 'ACAGT': 133874, 'ACAGC': 281692, 'ACAGG': 291937, 'ACTAA': 41231, 'ACTAT': 37310, 'ACTAC': 34457, 'ACTAG': 39448, 'ACTTA': 40693, 'ACTTT': 147129, 'ACTTC': 207696, 'ACTTG': 231005, 'ACTCA': 138108, 'ACTCT': 129406, 'ACTCC': 200732, 'ACTCG': 132374, 'ACTGA': 131100, 'ACTGT': 144526, 'ACTGC': 209519, 'ACTGG': 254893, 'ACCAA': 95940, 'ACCAT': 142118, 'ACCAC': 248610, 'ACCAG': 311270, 'ACCTA': 33272, 'ACCTT': 188037, 'ACCTC': 236385, 'ACCTG': 237641, 'ACCCA': 188294, 'ACCCT': 157897, 'ACCCC': 196392, 'ACCCG': 112832, 'ACCGA': 64032, 'ACCGT': 69737, 'ACCGC': 91683, 'ACCGG': 89893, 'ACGAA': 62903, 'ACGAT': 66108, 'ACGAC': 58559, 'ACGAG': 82974, 'ACGTA': 39298, 'ACGTT': 89896, 'ACGTC': 109043, 'ACGTG': 99687, 'ACGCA': 76371, 'ACGCT': 82588, 'ACGCC': 136698, 'ACGCG': 65357, 'ACGGA': 92355, 'ACGGT': 102428, 'ACGGC': 143623, 'ACGGG': 145788, 'AGAAA': 209489, 'AGAAT': 106415, 'AGAAC': 159142, 'AGAAG': 300714, 'AGATA': 87578, 'AGATT': 82020, 'AGATC': 130456, 'AGATG': 245008, 'AGACA': 153025, 'AGACT': 103128, 'AGACC': 177003, 'AGACG': 112883, 'AGAGA': 215952, 'AGAGT': 118546, 'AGAGC': 216366, 'AGAGG': 359103, 'AGTAA': 76247, 'AGTAT': 57975, 'AGTAC': 76539, 'AGTAG': 111893, 'AGTTA': 59502, 'AGTTT': 145256, 'AGTTC': 152547, 'AGTTG': 155277, 'AGTCA': 138587, 'AGTCT': 148944, 'AGTCC': 216760, 'AGTCG': 95921, 'AGTGA': 159074, 'AGTGT': 119833, 'AGTGC': 166309, 'AGTGG': 251462, 'AGCAA': 128341, 'AGCAT': 148951, 'AGCAC': 243188, 'AGCAG': 430065, 'AGCTA': 65331, 'AGCTT': 257556, 'AGCTC': 326943, 'AGCTG': 426920, 'AGCCA': 315747, 'AGCCT': 261102, 'AGCCC': 357535, 'AGCCG': 224516, 'AGCGA': 91457, 'AGCGT': 133461, 'AGCGC': 187208, 'AGCGG': 231759, 'AGGAA': 322270, 'AGGAT': 211712, 'AGGAC': 237848, 'AGGAG': 428130, 'AGGTA': 146492, 'AGGTT': 173202, 'AGGTC': 269830, 'AGGTG': 374210, 'AGGCA': 321058, 'AGGCT': 340488, 'AGGCC': 434927, 'AGGCG': 269894, 'AGGGA': 305068, 'AGGGT': 262787, 'AGGGC': 470895, 'AGGGG': 546150, 'TAAAA': 156635, 'TAAAT': 91744, 'TAAAC': 64227, 'TAAAG': 74724, 'TAATA': 51186, 'TAATT': 71472, 'TAATC': 64533, 'TAATG': 68960, 'TAACA': 69241, 'TAACT': 66180, 'TAACC': 45822, 'TAACG': 13774, 'TAAGA': 64441, 'TAAGT': 45497, 'TAAGC': 56413, 'TAAGG': 83405, 'TATAA': 49313, 'TATAT': 50031, 'TATAC': 31858, 'TATAG': 43661, 'TATTA': 38486, 'TATTT': 126141, 'TATTC': 69712, 'TATTG': 72949, 'TATCA': 59314, 'TATCT': 69141, 'TATCC': 61546, 'TATCG': 23678, 'TATGA': 49664, 'TATGT': 49661, 'TATGC': 36496, 'TATGG': 74756, 'TACAA': 85000, 'TACAT': 80126, 'TACAC': 81950, 'TACAG': 123117, 'TACTA': 23147, 'TACTT': 125682, 'TACTC': 92044, 'TACTG': 99774, 'TACCA': 70085, 'TACCT': 58443, 'TACCC': 66199, 'TACCG': 34571, 'TACGA': 21403, 'TACGT': 27607, 'TACGC': 22626, 'TACGG': 47324, 'TAGAA': 100454, 'TAGAT': 78735, 'TAGAC': 60426, 'TAGAG': 106901, 'TAGTA': 61456, 'TAGTT': 72968, 'TAGTC': 80726, 'TAGTG': 87004, 'TAGCA': 77761, 'TAGCT': 88755, 'TAGCC': 120971, 'TAGCG': 71204, 'TAGGA': 115719, 'TAGGT': 100152, 'TAGGC': 116383, 'TAGGG': 143378, 'TTAAA': 125333, 'TTAAT': 81747, 'TTAAC': 54927, 'TTAAG': 69891, 'TTATA': 49800, 'TTATT': 92177, 'TTATC': 59578, 'TTATG': 54248, 'TTACA': 88911, 'TTACT': 51206, 'TTACC': 45480, 'TTACG': 29155, 'TTAGA': 41817, 'TTAGT': 55017, 'TTAGC': 55244, 'TTAGG': 79403, 'TTTAA': 122455, 'TTTAT': 106244, 'TTTAC': 69130, 'TTTAG': 67840, 'TTTTA': 124074, 'TTTTT': 482719, 'TTTTC': 232337, 'TTTTG': 184667, 'TTTCA': 169318, 'TTTCT': 327070, 'TTTCC': 222065, 'TTTCG': 45685, 'TTTGA': 142314, 'TTTGT': 200536, 'TTTGC': 165127, 'TTTGG': 204437, 'TTCAA': 153765, 'TTCAT': 206531, 'TTCAC': 241613, 'TTCAG': 289580, 'TTCTA': 74004, 'TTCTT': 487081, 'TTCTC': 428951, 'TTCTG': 330024, 'TTCCA': 228185, 'TTCCT': 300769, 'TTCCC': 250800, 'TTCCG': 152074, 'TTCGA': 52499, 'TTCGT': 43309, 'TTCGC': 46565, 'TTCGG': 80346, 'TTGAA': 195017, 'TTGAT': 209558, 'TTGAC': 156410, 'TTGAG': 232670, 'TTGTA': 159589, 'TTGTT': 216296, 'TTGTC': 242599, 'TTGTG': 174280, 'TTGCA': 151196, 'TTGCT': 200695, 'TTGCC': 224951, 'TTGCG': 144843, 'TTGGA': 221745, 'TTGGT': 246462, 'TTGGC': 315154, 'TTGGG': 317999, 'TCAAA': 182449, 'TCAAT': 140787, 'TCAAC': 83345, 'TCAAG': 125998, 'TCATA': 104059, 'TCATT': 125252, 'TCATC': 321126, 'TCATG': 221481, 'TCACA': 212264, 'TCACT': 191204, 'TCACC': 240326, 'TCACG': 117561, 'TCAGA': 170880, 'TCAGT': 162701, 'TCAGC': 351470, 'TCAGG': 358735, 'TCTAA': 49546, 'TCTAT': 57246, 'TCTAC': 67537, 'TCTAG': 68498, 'TCTTA': 79918, 'TCTTT': 258838, 'TCTTC': 518499, 'TCTTG': 483813, 'TCTCA': 187975, 'TCTCT': 285471, 'TCTCC': 492603, 'TCTCG': 215181, 'TCTGA': 185752, 'TCTGT': 216182, 'TCTGC': 340163, 'TCTGG': 455748, 'TCCAA': 158817, 'TCCAT': 209886, 'TCCAC': 373768, 'TCCAG': 574500, 'TCCTA': 63973, 'TCCTT': 378540, 'TCCTC': 636941, 'TCCTG': 395771, 'TCCCA': 285095, 'TCCCT': 206281, 'TCCCC': 324206, 'TCCCG': 254391, 'TCCGA': 93501, 'TCCGT': 129186, 'TCCGC': 187644, 'TCCGG': 234096, 'TCGAA': 111847, 'TCGAT': 102428, 'TCGAC': 44952, 'TCGAG': 68573, 'TCGTA': 87478, 'TCGTT': 79142, 'TCGTC': 163203, 'TCGTG': 110170, 'TCGCA': 88051, 'TCGCT': 113610, 'TCGCC': 142001, 'TCGCG': 78374, 'TCGGA': 99405, 'TCGGT': 100165, 'TCGGC': 203645, 'TCGGG': 222538, 'TGAAA': 132771, 'TGAAT': 106964, 'TGAAC': 154097, 'TGAAG': 326467, 'TGATA': 65512, 'TGATT': 74162, 'TGATC': 170262, 'TGATG': 329073, 'TGACA': 157621, 'TGACT': 118249, 'TGACC': 196885, 'TGACG': 148947, 'TGAGA': 191741, 'TGAGT': 135391, 'TGAGC': 284846, 'TGAGG': 391472, 'TGTAA': 87098, 'TGTAT': 72417, 'TGTAC': 114509, 'TGTAG': 213017, 'TGTTA': 72565, 'TGTTT': 177502, 'TGTTC': 202746, 'TGTTG': 288433, 'TGTCA': 194448, 'TGTCT': 225366, 'TGTCC': 340529, 'TGTCG': 137578, 'TGTGA': 145279, 'TGTGT': 180920, 'TGTGC': 194239, 'TGTGG': 314770, 'TGCAA': 121878, 'TGCAT': 148214, 'TGCAC': 201820, 'TGCAG': 394713, 'TGCTA': 54500, 'TGCTT': 234019, 'TGCTC': 305052, 'TGCTG': 484031, 'TGCCA': 268639, 'TGCCT': 250861, 'TGCCC': 346908, 'TGCCG': 242798, 'TGCGA': 57482, 'TGCGT': 92388, 'TGCGC': 163193, 'TGCGG': 275137, 'TGGAA': 260240, 'TGGAT': 184042, 'TGGAC': 211769, 'TGGAG': 427464, 'TGGTA': 131994, 'TGGTT': 196313, 'TGGTC': 336809, 'TGGTG': 411537, 'TGGCA': 324361, 'TGGCT': 351959, 'TGGCC': 518361, 'TGGCG': 275211, 'TGGGA': 312690, 'TGGGT': 305610, 'TGGGC': 471982, 'TGGGG': 683154, 'CAAAA': 200060, 'CAAAT': 116742, 'CAAAC': 177517, 'CAAAG': 231133, 'CAATA': 63827, 'CAATT': 79047, 'CAATC': 104431, 'CAATG': 171064, 'CAACA': 133004, 'CAACT': 98366, 'CAACC': 105798, 'CAACG': 52528, 'CAAGA': 124184, 'CAAGT': 126134, 'CAAGC': 126325, 'CAAGG': 221324, 'CATAA': 73841, 'CATAT': 55352, 'CATAC': 98167, 'CATAG': 144289, 'CATTA': 67122, 'CATTT': 134562, 'CATTC': 158298, 'CATTG': 137956, 'CATCA': 240714, 'CATCT': 300461, 'CATCC': 264677, 'CATCG': 95202, 'CATGA': 216304, 'CATGT': 190862, 'CATGC': 183068, 'CATGG': 295781, 'CACAA': 189587, 'CACAT': 191353, 'CACAC': 235149, 'CACAG': 359215, 'CACTA': 52704, 'CACTT': 171784, 'CACTC': 192045, 'CACTG': 327966, 'CACCA': 392389, 'CACCT': 338841, 'CACCC': 266323, 'CACCG': 141751, 'CACGA': 125469, 'CACGT': 169018, 'CACGC': 180052, 'CACGG': 222396, 'CAGAA': 249661, 'CAGAT': 176473, 'CAGAC': 190559, 'CAGAG': 335420, 'CAGTA': 99383, 'CAGTT': 171201, 'CAGTC': 187545, 'CAGTG': 272512, 'CAGCA': 489311, 'CAGCT': 536341, 'CAGCC': 506684, 'CAGCG': 299651, 'CAGGA': 493152, 'CAGGT': 408481, 'CAGGC': 549385, 'CAGGG': 681212, 'CTAAA': 56324, 'CTAAT': 35967, 'CTAAC': 43358, 'CTAAG': 56578, 'CTATA': 25213, 'CTATT': 57582, 'CTATC': 47868, 'CTATG': 47780, 'CTACA': 62929, 'CTACT': 54329, 'CTACC': 54737, 'CTACG': 29292, 'CTAGA': 64481, 'CTAGT': 26896, 'CTAGC': 52964, 'CTAGG': 88613, 'CTTAA': 78169, 'CTTAT': 50852, 'CTTAC': 39310, 'CTTAG': 72725, 'CTTTA': 117301, 'CTTTT': 208988, 'CTTTC': 207880, 'CTTTG': 236390, 'CTTCA': 412994, 'CTTCT': 579381, 'CTTCC': 412280, 'CTTCG': 85531, 'CTTGA': 344007, 'CTTGT': 315522, 'CTTGC': 325885, 'CTTGG': 503060, 'CTCAA': 159892, 'CTCAT': 232779, 'CTCAC': 181355, 'CTCAG': 346335, 'CTCTA': 75723, 'CTCTT': 306706, 'CTCTC': 266731, 'CTCTG': 382744, 'CTCCA': 561593, 'CTCCT': 636855, 'CTCCC': 370051, 'CTCCG': 264999, 'CTCGA': 144665, 'CTCGT': 212432, 'CTCGC': 215208, 'CTCGG': 306048, 'CTGAA': 179633, 'CTGAT': 115029, 'CTGAC': 172151, 'CTGAG': 313504, 'CTGTA': 128449, 'CTGTT': 181173, 'CTGTC': 277568, 'CTGTG': 313677, 'CTGCA': 399190, 'CTGCT': 473425, 'CTGCC': 453101, 'CTGCG': 197077, 'CTGGA': 394764, 'CTGGT': 368865, 'CTGGC': 471995, 'CTGGG': 737607, 'CCAAA': 205682, 'CCAAT': 82916, 'CCAAC': 128879, 'CCAAG': 199130, 'CCATA': 99375, 'CCATT': 143396, 'CCATC': 241526, 'CCATG': 311087, 'CCACA': 332983, 'CCACT': 232718, 'CCACC': 465601, 'CCACG': 293925, 'CCAGA': 307787, 'CCAGT': 230286, 'CCAGC': 630239, 'CCAGG': 804355, 'CCTAA': 45862, 'CCTAT': 48929, 'CCTAC': 42850, 'CCTAG': 63421, 'CCTTA': 58217, 'CCTTT': 180344, 'CCTTC': 397912, 'CCTTG': 447944, 'CCTCA': 319528, 'CCTCT': 343748, 'CCTCC': 659676, 'CCTCG': 319809, 'CCTGA': 210211, 'CCTGT': 217432, 'CCTGC': 411807, 'CCTGG': 588966, 'CCCAA': 183809, 'CCCAT': 214461, 'CCCAC': 332595, 'CCCAG': 572017, 'CCCTA': 55133, 'CCCTT': 242417, 'CCCTC': 331878, 'CCCTG': 369623, 'CCCCA': 399882, 'CCCCT': 273760, 'CCCCC': 372011, 'CCCCG': 308604, 'CCCGA': 147199, 'CCCGT': 159154, 'CCCGC': 270086, 'CCCGG': 397575, 'CCGAA': 91821, 'CCGAT': 80663, 'CCGAC': 91164, 'CCGAG': 185679, 'CCGTA': 74383, 'CCGTT': 107312, 'CCGTC': 184423, 'CCGTG': 191596, 'CCGCA': 177865, 'CCGCT': 240515, 'CCGCC': 338712, 'CCGCG': 174866, 'CCGGA': 176159, 'CCGGT': 175144, 'CCGGC': 325719, 'CCGGG': 406334, 'CGAAA': 48978, 'CGAAT': 45990, 'CGAAC': 69402, 'CGAAG': 163692, 'CGATA': 22062, 'CGATT': 33125, 'CGATC': 88187, 'CGATG': 192698, 'CGACA': 61344, 'CGACT': 68790, 'CGACC': 83645, 'CGACG': 55605, 'CGAGA': 91637, 'CGAGT': 70549, 'CGAGC': 111167, 'CGAGG': 203275, 'CGTAA': 22357, 'CGTAT': 27170, 'CGTAC': 57243, 'CGTAG': 146235, 'CGTTA': 20568, 'CGTTT': 79656, 'CGTTC': 123891, 'CGTTG': 145157, 'CGTCA': 110541, 'CGTCT': 155203, 'CGTCC': 213573, 'CGTCG': 138734, 'CGTGA': 99810, 'CGTGT': 105235, 'CGTGC': 149149, 'CGTGG': 239868, 'CGCAA': 43260, 'CGCAT': 68723, 'CGCAC': 130159, 'CGCAG': 279312, 'CGCTA': 20367, 'CGCTT': 134473, 'CGCTC': 224585, 'CGCTG': 258404, 'CGCCA': 186152, 'CGCCT': 186131, 'CGCCC': 279204, 'CGCCG': 222468, 'CGCGA': 60060, 'CGCGT': 68974, 'CGCGC': 134671, 'CGCGG': 209801, 'CGGAA': 114934, 'CGGAT': 135905, 'CGGAC': 113789, 'CGGAG': 205237, 'CGGTA': 99612, 'CGGTT': 93720, 'CGGTC': 151118, 'CGGTG': 233060, 'CGGCA': 192988, 'CGGCT': 227401, 'CGGCC': 390755, 'CGGCG': 265103, 'CGGGA': 205556, 'CGGGT': 147693, 'CGGGC': 370136, 'CGGGG': 422218, 'GAAAA': 162422, 'GAAAT': 136077, 'GAAAC': 122910, 'GAAAG': 174512, 'GAATA': 65887, 'GAATT': 99251, 'GAATC': 108436, 'GAATG': 119425, 'GAACA': 161574, 'GAACT': 219773, 'GAACC': 130814, 'GAACG': 58573, 'GAAGA': 280557, 'GAAGT': 232720, 'GAAGC': 299282, 'GAAGG': 422579, 'GATAA': 59800, 'GATAT': 50683, 'GATAC': 56155, 'GATAG': 68406, 'GATTA': 37925, 'GATTT': 107030, 'GATTC': 86266, 'GATTG': 64552, 'GATCA': 148958, 'GATCT': 266293, 'GATCC': 142675, 'GATCG': 41793, 'GATGA': 289025, 'GATGT': 265025, 'GATGC': 225272, 'GATGG': 356022, 'GACAA': 108543, 'GACAT': 124322, 'GACAC': 154128, 'GACAG': 245679, 'GACTA': 34355, 'GACTT': 151367, 'GACTC': 163950, 'GACTG': 143671, 'GACCA': 215437, 'GACCT': 183481, 'GACCC': 200323, 'GACCG': 93500, 'GACGA': 94640, 'GACGT': 102994, 'GACGC': 113502, 'GACGG': 152733, 'GAGAA': 238394, 'GAGAT': 149612, 'GAGAC': 179737, 'GAGAG': 272722, 'GAGTA': 68425, 'GAGTT': 127572, 'GAGTC': 179805, 'GAGTG': 172587, 'GAGCA': 230329, 'GAGCT': 289678, 'GAGCC': 307129, 'GAGCG': 166442, 'GAGGA': 381432, 'GAGGT': 280205, 'GAGGC': 434948, 'GAGGG': 496150, 'GTAAA': 71057, 'GTAAT': 74239, 'GTAAC': 50257, 'GTAAG': 60550, 'GTATA': 39287, 'GTATT': 80797, 'GTATC': 55597, 'GTATG': 58153, 'GTACA': 125599, 'GTACT': 148413, 'GTACC': 77228, 'GTACG': 41221, 'GTAGA': 161633, 'GTAGT': 147170, 'GTAGC': 163258, 'GTAGG': 214586, 'GTTAA': 62633, 'GTTAT': 51066, 'GTTAC': 48970, 'GTTAG': 45813, 'GTTTA': 54554, 'GTTTT': 166715, 'GTTTC': 183936, 'GTTTG': 157459, 'GTTCA': 191633, 'GTTCT': 264153, 'GTTCC': 177766, 'GTTCG': 51637, 'GTTGA': 213928, 'GTTGT': 201196, 'GTTGC': 150171, 'GTTGG': 289898, 'GTCAA': 125304, 'GTCAT': 163074, 'GTCAC': 217017, 'GTCAG': 233714, 'GTCTA': 52630, 'GTCTT': 247308, 'GTCTC': 252951, 'GTCTG': 255093, 'GTCCA': 328751, 'GTCCT': 349343, 'GTCCC': 326730, 'GTCCG': 154406, 'GTCGA': 92385, 'GTCGT': 130696, 'GTCGC': 107728, 'GTCGG': 182077, 'GTGAA': 173563, 'GTGAT': 137064, 'GTGAC': 164611, 'GTGAG': 247296, 'GTGTA': 93670, 'GTGTT': 145733, 'GTGTC': 184037, 'GTGTG': 208850, 'GTGCA': 200428, 'GTGCT': 263144, 'GTGCC': 245106, 'GTGCG': 127525, 'GTGGA': 294592, 'GTGGT': 246832, 'GTGGC': 401982, 'GTGGG': 491016, 'GCAAA': 150811, 'GCAAT': 88449, 'GCAAC': 97065, 'GCAAG': 145783, 'GCATA': 83410, 'GCATT': 100351, 'GCATC': 193771, 'GCATG': 198369, 'GCACA': 232400, 'GCACT': 201787, 'GCACC': 253527, 'GCACG': 177768, 'GCAGA': 281335, 'GCAGT': 200595, 'GCAGC': 575268, 'GCAGG': 687140, 'GCTAA': 55544, 'GCTAT': 34437, 'GCTAC': 58396, 'GCTAG': 61654, 'GCTTA': 62835, 'GCTTT': 185868, 'GCTTC': 365117, 'GCTTG': 320353, 'GCTCA': 271429, 'GCTCT': 270974, 'GCTCC': 489198, 'GCTCG': 215080, 'GCTGA': 249608, 'GCTGT': 318361, 'GCTGC': 563677, 'GCTGG': 680416, 'GCCAA': 180504, 'GCCAT': 226123, 'GCCAC': 372393, 'GCCAG': 513857, 'GCCTA': 49227, 'GCCTT': 273539, 'GCCTC': 437964, 'GCCTG': 425825, 'GCCCA': 427681, 'GCCCT': 359942, 'GCCCC': 487497, 'GCCCG': 311406, 'GCCGA': 143953, 'GCCGT': 197190, 'GCCGC': 387809, 'GCCGG': 372203, 'GCGAA': 61670, 'GCGAT': 84030, 'GCGAC': 75545, 'GCGAG': 142613, 'GCGTA': 50679, 'GCGTT': 91462, 'GCGTC': 162053, 'GCGTG': 193614, 'GCGCA': 175989, 'GCGCT': 197419, 'GCGCC': 264952, 'GCGCG': 159769, 'GCGGA': 199797, 'GCGGT': 197327, 'GCGGC': 408907, 'GCGGG': 384343, 'GGAAA': 204960, 'GGAAT': 132855, 'GGAAC': 188842, 'GGAAG': 443942, 'GGATA': 58981, 'GGATT': 105227, 'GGATC': 211664, 'GGATG': 364549, 'GGACA': 256282, 'GGACT': 201533, 'GGACC': 239373, 'GGACG': 148107, 'GGAGA': 337850, 'GGAGT': 221038, 'GGAGC': 382114, 'GGAGG': 642513, 'GGTAA': 69879, 'GGTAT': 75954, 'GGTAC': 144978, 'GGTAG': 215598, 'GGTTA': 55211, 'GGTTT': 160258, 'GGTTC': 205399, 'GGTTG': 267178, 'GGTCA': 290256, 'GGTCT': 275552, 'GGTCC': 390336, 'GGTCG': 143335, 'GGTGA': 313453, 'GGTGT': 222104, 'GGTGC': 325643, 'GGTGG': 630094, 'GGCAA': 188890, 'GGCAT': 207291, 'GGCAC': 292938, 'GGCAG': 640772, 'GGCTA': 70389, 'GGCTT': 306836, 'GGCTC': 392584, 'GGCTG': 639218, 'GGCCA': 520460, 'GGCCT': 485051, 'GGCCC': 619815, 'GGCCG': 422144, 'GGCGA': 152481, 'GGCGT': 201061, 'GGCGC': 315388, 'GGCGG': 480911, 'GGGAA': 270640, 'GGGAT': 201527, 'GGGAC': 283628, 'GGGAG': 526624, 'GGGTA': 126135, 'GGGTT': 223694, 'GGGTC': 344815, 'GGGTG': 468156, 'GGGCA': 484576, 'GGGCT': 480178, 'GGGCC': 717982, 'GGGCG': 354061, 'GGGGA': 451209, 'GGGGT': 439887, 'GGGGC': 731341, 'GGGGG': 744289}, 'overrepresented_sequences': {}} {'total_reads': 3121402, 'total_bases': 180842593, 'q20_bases': 168648189, 'q30_bases': 157956596, 'total_cycles': 58, 'quality_curves': {'A': [36.8218, 36.8259, 36.834, 36.8303, 36.8484, 36.819, 36.7902, 36.7507, 36.6975, 36.666, 36.7202, 36.7018, 36.6554, 36.6235, 36.5613, 36.5624, 36.4655, 36.3417, 36.2533, 36.2224, 36.1995, 36.1545, 36.0667, 36.0628, 35.9302, 35.8976, 35.7804, 35.6989, 35.6818, 35.4122, 35.3569, 35.1594, 34.9494, 34.771, 34.7739, 34.5785, 34.5579, 34.3542, 34.0721, 33.7407, 33.6473, 33.2084, 33.1549, 33.0968, 32.9768, 32.7564, 32.4251, 32.1308, 31.7443, 31.6913, 31.3085, 30.8732, 30.4028, 30.0923, 30.0632, 29.3697, 28.8966, 28.2618], 'T': [37.1, 37.156, 37.13, 37.099, 37.116, 37.0921, 37.046, 37.0066, 36.946, 36.8959, 36.94, 36.9089, 36.857, 36.8392, 36.7497, 36.761, 36.6749, 36.545, 36.4719, 36.4395, 36.3815, 36.3629, 36.252, 36.2236, 36.0763, 36.0957, 35.976, 35.8681, 35.805, 35.6028, 35.5056, 35.3227, 35.1382, 34.9596, 34.9411, 34.7804, 34.7088, 34.5212, 34.1571, 33.9175, 33.7568, 33.3974, 33.3212, 33.3239, 33.2081, 32.9107, 32.5765, 32.278, 31.8944, 31.8436, 31.5, 31.0862, 30.6439, 30.4044, 30.2562, 29.7006, 29.1927, 28.5967], 'C': [37.1865, 37.1973, 37.2122, 37.2153, 37.2061, 37.1983, 37.1791, 37.1736, 37.1331, 37.1102, 37.1142, 37.1252, 37.1119, 37.0633, 37.0651, 37.0423, 36.9972, 36.9331, 36.8668, 36.7775, 36.8194, 36.7891, 36.7359, 36.7174, 36.6354, 36.5885, 36.5535, 36.4463, 36.4165, 36.3084, 36.1964, 36.0429, 35.9188, 35.7835, 35.7161, 35.6117, 35.54, 35.4426, 35.2216, 34.9009, 34.7406, 34.4288, 34.3394, 34.1919, 34.1255, 33.8696, 33.5392, 33.1703, 32.796, 32.7033, 32.3827, 31.9677, 31.4877, 31.3009, 30.9969, 30.3476, 29.868, 29.2235], 'G': [36.8192, 36.8624, 36.8691, 36.8419, 36.843, 36.8413, 36.8083, 36.7589, 36.7236, 36.6815, 36.7117, 36.7111, 36.6717, 36.6298, 36.5848, 36.5715, 36.4876, 36.3958, 36.2971, 36.2306, 36.2181, 36.1724, 36.0931, 36.092, 35.9316, 35.9244, 35.8358, 35.7164, 35.6904, 35.5189, 35.3891, 35.2196, 35.0672, 34.8735, 34.8618, 34.6924, 34.6399, 34.4657, 34.1698, 33.9221, 33.6657, 33.3208, 33.2326, 33.1354, 33.0794, 32.8107, 32.4367, 32.136, 31.7307, 31.6313, 31.2827, 30.8586, 30.3647, 30.1167, 29.8888, 29.2687, 28.6682, 28.125], 'mean': [36.9859, 37.0111, 37.0147, 36.9983, 37.004, 36.9908, 36.9561, 36.9287, 36.8835, 36.8461, 36.8771, 36.8702, 36.8322, 36.797, 36.7556, 36.747, 36.6698, 36.5726, 36.4905, 36.4313, 36.4214, 36.3877, 36.3083, 36.3, 36.1687, 36.15, 36.0671, 35.9595, 35.9255, 35.7515, 35.6444, 35.4679, 35.3126, 35.1377, 35.1103, 34.9629, 34.9077, 34.7435, 34.4653, 34.1745, 33.9996, 33.6493, 33.5645, 33.4791, 33.398, 33.1378, 32.792, 32.4769, 32.087, 32.0056, 31.6678, 31.2374, 30.7653, 30.5323, 30.3324, 29.7017, 29.1881, 28.5792]}, 'content_curves': {'A': [0.212359, 0.218576, 0.214103, 0.218433, 0.226007, 0.215036, 0.218302, 0.221014, 0.217073, 0.219851, 0.224434, 0.219972, 0.219449, 0.223135, 0.214093, 0.217515, 0.222694, 0.217579, 0.219524, 0.223028, 0.217902, 0.218934, 0.222409, 0.215086, 0.220852, 0.222208, 0.215428, 0.219245, 0.22244, 0.217712, 0.221602, 0.223903, 0.216177, 0.219767, 0.223579, 0.215051, 0.217048, 0.223834, 0.218897, 0.220468, 0.226004, 0.214743, 0.219913, 0.221253, 0.217578, 0.218145, 0.22343, 0.216157, 0.219524, 0.221988, 0.213698, 0.216841, 0.219907, 0.213802, 0.218653, 0.221899, 0.213374, 0.217964], 'T': [0.20497, 0.198901, 0.198103, 0.191899, 0.195378, 0.192832, 0.190693, 0.19983, 0.195704, 0.193592, 0.200299, 0.195402, 0.194558, 0.198841, 0.197327, 0.195517, 0.197369, 0.199305, 0.194503, 0.198345, 0.193702, 0.191106, 0.197918, 0.1917, 0.193864, 0.197641, 0.195482, 0.194589, 0.199205, 0.193048, 0.192071, 0.197978, 0.194463, 0.194438, 0.197012, 0.196637, 0.191538, 0.195581, 0.193151, 0.191133, 0.193708, 0.193203, 0.192627, 0.198393, 0.191787, 0.191716, 0.195548, 0.191851, 0.190557, 0.196543, 0.1927, 0.18986, 0.19615, 0.193483, 0.191243, 0.194459, 0.192134, 0.190774], 'C': [0.298101, 0.296782, 0.29697, 0.293511, 0.293003, 0.296809, 0.294818, 0.294463, 0.297946, 0.295208, 0.292552, 0.295772, 0.295566, 0.294482, 0.298364, 0.298162, 0.295699, 0.298396, 0.296746, 0.295868, 0.295219, 0.296498, 0.294978, 0.303162, 0.298087, 0.297723, 0.300728, 0.297894, 0.294958, 0.303779, 0.297653, 0.2932, 0.30191, 0.296973, 0.295578, 0.302062, 0.302629, 0.298845, 0.303598, 0.300653, 0.298087, 0.304928, 0.299981, 0.300052, 0.302592, 0.301998, 0.299853, 0.304287, 0.302394, 0.297806, 0.307047, 0.302707, 0.300921, 0.308346, 0.302502, 0.302747, 0.308674, 0.304398], 'G': [0.284545, 0.28571, 0.290813, 0.296157, 0.285612, 0.295323, 0.296105, 0.284694, 0.289277, 0.291349, 0.282714, 0.288854, 0.290368, 0.283523, 0.290216, 0.288806, 0.284223, 0.284676, 0.289226, 0.282739, 0.293125, 0.293461, 0.284695, 0.290038, 0.287184, 0.282429, 0.288362, 0.288273, 0.283397, 0.285455, 0.288667, 0.28492, 0.28745, 0.288815, 0.283831, 0.28625, 0.288785, 0.28174, 0.284354, 0.287715, 0.282201, 0.287125, 0.287476, 0.280231, 0.288032, 0.288142, 0.281168, 0.287705, 0.287525, 0.283662, 0.286554, 0.29048, 0.283004, 0.284369, 0.287602, 0.280895, 0.285817, 0.286865], 'N': [2.49888e-05, 3.13961e-05, 1.12129e-05, 0, 0, 0, 8.26552e-05, 0, 0, 0, 0, 0, 5.9909e-05, 1.85814e-05, 0, 0, 1.53777e-05, 4.38905e-05, 9.61107e-07, 2.01832e-05, 5.15794e-05, 0, 0, 1.44166e-05, 1.34555e-05, 0, 0, 0, 0, 5.76673e-06, 7.04825e-06, 0, 0, 7.0493e-06, 0, 0, 0, 0, 0, 3.10924e-05, 0, 0, 2.24439e-06, 7.02255e-05, 1.05834e-05, 0, 6.41634e-07, 0, 0, 6.42208e-07, 3.21279e-07, 0.000113153, 1.73712e-05, 0, 0, 0, 0, 0], 'GC': [0.582646, 0.582491, 0.587783, 0.589668, 0.578615, 0.592132, 0.590922, 0.579156, 0.587223, 0.586557, 0.575266, 0.584626, 0.585934, 0.578006, 0.58858, 0.586968, 0.579922, 0.583071, 0.585972, 0.578607, 0.588344, 0.589959, 0.579673, 0.5932, 0.585271, 0.580152, 0.58909, 0.586167, 0.578355, 0.589235, 0.58632, 0.578119, 0.58936, 0.585788, 0.579409, 0.588312, 0.591415, 0.580585, 0.587952, 0.588368, 0.580288, 0.592053, 0.587457, 0.580284, 0.590624, 0.59014, 0.581021, 0.591992, 0.589919, 0.581468, 0.593601, 0.593187, 0.583925, 0.592715, 0.590104, 0.583642, 0.594491, 0.591262]}, 'kmer_count': {'AAAAA': 259663, 'AAAAT': 119040, 'AAAAC': 144063, 'AAAAG': 167210, 'AAATA': 91942, 'AAATT': 94679, 'AAATC': 83197, 'AAATG': 113579, 'AAACA': 159349, 'AAACT': 113403, 'AAACC': 138377, 'AAACG': 64317, 'AAAGA': 224554, 'AAAGT': 123354, 'AAAGC': 157729, 'AAAGG': 149882, 'AATAA': 60199, 'AATAT': 57727, 'AATAC': 60861, 'AATAG': 39571, 'AATTA': 51960, 'AATTT': 97805, 'AATTC': 75041, 'AATTG': 58379, 'AATCA': 62101, 'AATCT': 73259, 'AATCC': 93001, 'AATCG': 24412, 'AATGA': 98215, 'AATGT': 105143, 'AATGC': 87836, 'AATGG': 123911, 'AACAA': 191922, 'AACAT': 160079, 'AACAC': 112835, 'AACAG': 163508, 'AACTA': 56254, 'AACTT': 121441, 'AACTC': 108272, 'AACTG': 146396, 'AACCA': 163637, 'AACCT': 149970, 'AACCC': 177331, 'AACCG': 74611, 'AACGA': 67373, 'AACGT': 71322, 'AACGC': 76927, 'AACGG': 89153, 'AAGAA': 420233, 'AAGAT': 237512, 'AAGAC': 215569, 'AAGAG': 269669, 'AAGTA': 101655, 'AAGTT': 146889, 'AAGTC': 143339, 'AAGTG': 145655, 'AAGCA': 199652, 'AAGCT': 230257, 'AAGCC': 255466, 'AAGCG': 128422, 'AAGGA': 316781, 'AAGGT': 175586, 'AAGGC': 239609, 'AAGGG': 199033, 'ATAAA': 74401, 'ATAAT': 36155, 'ATAAC': 41550, 'ATAAG': 40996, 'ATATA': 38345, 'ATATT': 56382, 'ATATC': 48111, 'ATATG': 46560, 'ATACA': 63444, 'ATACT': 47961, 'ATACC': 52968, 'ATACG': 22917, 'ATAGA': 42982, 'ATAGT': 32066, 'ATAGC': 32496, 'ATAGG': 36507, 'ATTAA': 59346, 'ATTAT': 45340, 'ATTAC': 61393, 'ATTAG': 24851, 'ATTTA': 63091, 'ATTTT': 141069, 'ATTTC': 109831, 'ATTTG': 89022, 'ATTCA': 93942, 'ATTCT': 92712, 'ATTCC': 95612, 'ATTCG': 43612, 'ATTGA': 114536, 'ATTGT': 82242, 'ATTGC': 75638, 'ATTGG': 65318, 'ATCAA': 173648, 'ATCAT': 150052, 'ATCAC': 114333, 'ATCAG': 103184, 'ATCTA': 65230, 'ATCTT': 137758, 'ATCTC': 134573, 'ATCTG': 152382, 'ATCCA': 163988, 'ATCCT': 184160, 'ATCCC': 164246, 'ATCCG': 124611, 'ATCGA': 96071, 'ATCGT': 60723, 'ATCGC': 67975, 'ATCGG': 63360, 'ATGAA': 183798, 'ATGAT': 148459, 'ATGAC': 138291, 'ATGAG': 204123, 'ATGTA': 63266, 'ATGTT': 92863, 'ATGTC': 126148, 'ATGTG': 169916, 'ATGCA': 124977, 'ATGCT': 129494, 'ATGCC': 189800, 'ATGCG': 61382, 'ATGGA': 195278, 'ATGGT': 137759, 'ATGGC': 218642, 'ATGGG': 178473, 'ACAAA': 163345, 'ACAAT': 65458, 'ACAAC': 161123, 'ACAAG': 292724, 'ACATA': 39874, 'ACATT': 96749, 'ACATC': 218154, 'ACATG': 170290, 'ACACA': 150989, 'ACACT': 94735, 'ACACC': 177979, 'ACACG': 89074, 'ACAGA': 193706, 'ACAGT': 123128, 'ACAGC': 266367, 'ACAGG': 186052, 'ACTAA': 42784, 'ACTAT': 57618, 'ACTAC': 121618, 'ACTAG': 22563, 'ACTTA': 40819, 'ACTTT': 119787, 'ACTTC': 194818, 'ACTTG': 90793, 'ACTCA': 109261, 'ACTCT': 107896, 'ACTCC': 185392, 'ACTCG': 62003, 'ACTGA': 139485, 'ACTGT': 123221, 'ACTGC': 183441, 'ACTGG': 204999, 'ACCAA': 213075, 'ACCAT': 197273, 'ACCAC': 195882, 'ACCAG': 321743, 'ACCTA': 84540, 'ACCTT': 139252, 'ACCTC': 229158, 'ACCTG': 356078, 'ACCCA': 242933, 'ACCCT': 221996, 'ACCCC': 342497, 'ACCCG': 128957, 'ACCGA': 84201, 'ACCGT': 79747, 'ACCGC': 184596, 'ACCGG': 140089, 'ACGAA': 40433, 'ACGAT': 51357, 'ACGAC': 104749, 'ACGAG': 181957, 'ACGTA': 22729, 'ACGTT': 30620, 'ACGTC': 81391, 'ACGTG': 145389, 'ACGCA': 91916, 'ACGCT': 133034, 'ACGCC': 182410, 'ACGCG': 65147, 'ACGGA': 127025, 'ACGGT': 59217, 'ACGGC': 172501, 'ACGGG': 128461, 'AGAAA': 263776, 'AGAAT': 114419, 'AGAAC': 217573, 'AGAAG': 507257, 'AGATA': 58939, 'AGATT': 107941, 'AGATC': 227163, 'AGATG': 267399, 'AGACA': 193412, 'AGACT': 131947, 'AGACC': 233426, 'AGACG': 149415, 'AGAGA': 251922, 'AGAGT': 134306, 'AGAGC': 256989, 'AGAGG': 291916, 'AGTAA': 41175, 'AGTAT': 72461, 'AGTAC': 133479, 'AGTAG': 53040, 'AGTTA': 52731, 'AGTTT': 121781, 'AGTTC': 193311, 'AGTTG': 85177, 'AGTCA': 114004, 'AGTCT': 99825, 'AGTCC': 180973, 'AGTCG': 65053, 'AGTGA': 167257, 'AGTGT': 107848, 'AGTGC': 168299, 'AGTGG': 225228, 'AGCAA': 167943, 'AGCAT': 113365, 'AGCAC': 213969, 'AGCAG': 423668, 'AGCTA': 77926, 'AGCTT': 128034, 'AGCTC': 252665, 'AGCTG': 476947, 'AGCCA': 322510, 'AGCCT': 300248, 'AGCCC': 410492, 'AGCCG': 219355, 'AGCGA': 107982, 'AGCGT': 87679, 'AGCGC': 184296, 'AGCGG': 250328, 'AGGAA': 243349, 'AGGAT': 161473, 'AGGAC': 304902, 'AGGAG': 559386, 'AGGTA': 62646, 'AGGTT': 100545, 'AGGTC': 169330, 'AGGTG': 305233, 'AGGCA': 212542, 'AGGCT': 223517, 'AGGCC': 422543, 'AGGCG': 179489, 'AGGGA': 179113, 'AGGGT': 140483, 'AGGGC': 297084, 'AGGGG': 217488, 'TAAAA': 92551, 'TAAAT': 49840, 'TAAAC': 47918, 'TAAAG': 93753, 'TAATA': 23226, 'TAATT': 50449, 'TAATC': 32795, 'TAATG': 54701, 'TAACA': 56813, 'TAACT': 51296, 'TAACC': 44591, 'TAACG': 15552, 'TAAGA': 66762, 'TAAGT': 35581, 'TAAGC': 45739, 'TAAGG': 49209, 'TATAA': 36281, 'TATAT': 46603, 'TATAC': 29312, 'TATAG': 24436, 'TATTA': 38667, 'TATTT': 112831, 'TATTC': 50319, 'TATTG': 53471, 'TATCA': 56084, 'TATCT': 73183, 'TATCC': 57906, 'TATCG': 18881, 'TATGA': 91829, 'TATGT': 72618, 'TATGC': 71877, 'TATGG': 83381, 'TACAA': 140945, 'TACAT': 84192, 'TACAC': 79696, 'TACAG': 110847, 'TACTA': 49054, 'TACTT': 73080, 'TACTC': 56189, 'TACTG': 96787, 'TACCA': 116071, 'TACCT': 123411, 'TACCC': 102996, 'TACCG': 84736, 'TACGA': 84285, 'TACGT': 33164, 'TACGC': 57634, 'TACGG': 62159, 'TAGAA': 53176, 'TAGAT': 32532, 'TAGAC': 40700, 'TAGAG': 72020, 'TAGTA': 24061, 'TAGTT': 29842, 'TAGTC': 33175, 'TAGTG': 50315, 'TAGCA': 45105, 'TAGCT': 54506, 'TAGCC': 59565, 'TAGCG': 20813, 'TAGGA': 56113, 'TAGGT': 27471, 'TAGGC': 39728, 'TAGGG': 42652, 'TTAAA': 88751, 'TTAAT': 49720, 'TTAAC': 48744, 'TTAAG': 57959, 'TTATA': 39325, 'TTATT': 84214, 'TTATC': 45856, 'TTATG': 64885, 'TTACA': 68124, 'TTACT': 62710, 'TTACC': 54920, 'TTACG': 17389, 'TTAGA': 39603, 'TTAGT': 34268, 'TTAGC': 47358, 'TTAGG': 38350, 'TTTAA': 90998, 'TTTAT': 102443, 'TTTAC': 51674, 'TTTAG': 47278, 'TTTTA': 123126, 'TTTTT': 258833, 'TTTTC': 127963, 'TTTTG': 153582, 'TTTCA': 121055, 'TTTCT': 167643, 'TTTCC': 159193, 'TTTCG': 48671, 'TTTGA': 149585, 'TTTGT': 147247, 'TTTGC': 129898, 'TTTGG': 169131, 'TTCAA': 170860, 'TTCAT': 127933, 'TTCAC': 149400, 'TTCAG': 171751, 'TTCTA': 91309, 'TTCTT': 157150, 'TTCTC': 204347, 'TTCTG': 202454, 'TTCCA': 198795, 'TTCCT': 266055, 'TTCCC': 212908, 'TTCCG': 105593, 'TTCGA': 96163, 'TTCGT': 63589, 'TTCGC': 58875, 'TTCGG': 90589, 'TTGAA': 116706, 'TTGAT': 78989, 'TTGAC': 109993, 'TTGAG': 134198, 'TTGTA': 63203, 'TTGTT': 93568, 'TTGTC': 98030, 'TTGTG': 154255, 'TTGCA': 100803, 'TTGCT': 115957, 'TTGCC': 149468, 'TTGCG': 45421, 'TTGGA': 132792, 'TTGGT': 81313, 'TTGGC': 142607, 'TTGGG': 149284, 'TCAAA': 133474, 'TCAAT': 63175, 'TCAAC': 183061, 'TCAAG': 282367, 'TCATA': 43016, 'TCATT': 91940, 'TCATC': 230886, 'TCATG': 188001, 'TCACA': 123513, 'TCACT': 135208, 'TCACC': 263063, 'TCACG': 83859, 'TCAGA': 171201, 'TCAGT': 117561, 'TCAGC': 223532, 'TCAGG': 183376, 'TCTAA': 37068, 'TCTAT': 59821, 'TCTAC': 151687, 'TCTAG': 54190, 'TCTTA': 52755, 'TCTTT': 158941, 'TCTTC': 253912, 'TCTTG': 115262, 'TCTCA': 157593, 'TCTCT': 188875, 'TCTCC': 285462, 'TCTCG': 87465, 'TCTGA': 145756, 'TCTGT': 174157, 'TCTGC': 240930, 'TCTGG': 277444, 'TCCAA': 187005, 'TCCAT': 130237, 'TCCAC': 237292, 'TCCAG': 338664, 'TCCTA': 93229, 'TCCTT': 161498, 'TCCTC': 307144, 'TCCTG': 435076, 'TCCCA': 261609, 'TCCCT': 241887, 'TCCCC': 350568, 'TCCCG': 176972, 'TCCGA': 99921, 'TCCGT': 78527, 'TCCGC': 185756, 'TCCGG': 178385, 'TCGAA': 46462, 'TCGAT': 34574, 'TCGAC': 88784, 'TCGAG': 138097, 'TCGTA': 23416, 'TCGTT': 25111, 'TCGTC': 81483, 'TCGTG': 124125, 'TCGCA': 52185, 'TCGCT': 77832, 'TCGCC': 130695, 'TCGCG': 59375, 'TCGGA': 98524, 'TCGGT': 59738, 'TCGGC': 148535, 'TCGGG': 133997, 'TGAAA': 142875, 'TGAAT': 88342, 'TGAAC': 159221, 'TGAAG': 360466, 'TGATA': 50448, 'TGATT': 78357, 'TGATC': 137573, 'TGATG': 214585, 'TGACA': 159741, 'TGACT': 117097, 'TGACC': 261070, 'TGACG': 99104, 'TGAGA': 168131, 'TGAGT': 117237, 'TGAGC': 244818, 'TGAGG': 287825, 'TGTAA': 73587, 'TGTAT': 68415, 'TGTAC': 95234, 'TGTAG': 55691, 'TGTTA': 55517, 'TGTTT': 145066, 'TGTTC': 139562, 'TGTTG': 113520, 'TGTCA': 135683, 'TGTCT': 149812, 'TGTCC': 224327, 'TGTCG': 68506, 'TGTGA': 186729, 'TGTGT': 168264, 'TGTGC': 213972, 'TGTGG': 296508, 'TGCAA': 129484, 'TGCAT': 95060, 'TGCAC': 163691, 'TGCAG': 378087, 'TGCTA': 68284, 'TGCTT': 132917, 'TGCTC': 195819, 'TGCTG': 469395, 'TGCCA': 266485, 'TGCCT': 284187, 'TGCCC': 391332, 'TGCCG': 176410, 'TGCGA': 74711, 'TGCGT': 71977, 'TGCGC': 165263, 'TGCGG': 176874, 'TGGAA': 193949, 'TGGAT': 159402, 'TGGAC': 314158, 'TGGAG': 508383, 'TGGTA': 70139, 'TGGTT': 102081, 'TGGTC': 191762, 'TGGTG': 355252, 'TGGCA': 229792, 'TGGCT': 273334, 'TGGCC': 473483, 'TGGCG': 193402, 'TGGGA': 251755, 'TGGGT': 164281, 'TGGGC': 373412, 'TGGGG': 333016, 'CAAAA': 163636, 'CAAAT': 104943, 'CAAAC': 129956, 'CAAAG': 211041, 'CAATA': 46769, 'CAATT': 54421, 'CAATC': 61199, 'CAATG': 121128, 'CAACA': 240479, 'CAACT': 128904, 'CAACC': 209946, 'CAACG': 123326, 'CAAGA': 402027, 'CAAGT': 202323, 'CAAGC': 276833, 'CAAGG': 391514, 'CATAA': 43937, 'CATAT': 38266, 'CATAC': 51003, 'CATAG': 42220, 'CATTA': 53191, 'CATTT': 100458, 'CATTC': 102802, 'CATTG': 140666, 'CATCA': 279075, 'CATCT': 227750, 'CATCC': 291411, 'CATCG': 167392, 'CATGA': 196354, 'CATGT': 146568, 'CATGC': 177562, 'CATGG': 286857, 'CACAA': 143632, 'CACAT': 110368, 'CACAC': 167041, 'CACAG': 263725, 'CACTA': 72032, 'CACTT': 122901, 'CACTC': 131065, 'CACTG': 235972, 'CACCA': 348067, 'CACCT': 296150, 'CACCC': 355797, 'CACCG': 190063, 'CACGA': 89962, 'CACGT': 83043, 'CACGC': 178029, 'CACGG': 165752, 'CAGAA': 269179, 'CAGAT': 192747, 'CAGAC': 225969, 'CAGAG': 334948, 'CAGTA': 88958, 'CAGTT': 142308, 'CAGTC': 135258, 'CAGTG': 283505, 'CAGCA': 404224, 'CAGCT': 355815, 'CAGCC': 568066, 'CAGCG': 259309, 'CAGGA': 336137, 'CAGGT': 217020, 'CAGGC': 363399, 'CAGGG': 298374, 'CTAAA': 64304, 'CTAAT': 34702, 'CTAAC': 34326, 'CTAAG': 62638, 'CTATA': 34888, 'CTATT': 59734, 'CTATC': 67042, 'CTATG': 127613, 'CTACA': 180904, 'CTACT': 95371, 'CTACC': 202238, 'CTACG': 146347, 'CTAGA': 55709, 'CTAGT': 35116, 'CTAGC': 48959, 'CTAGG': 51293, 'CTTAA': 52784, 'CTTAT': 51968, 'CTTAC': 45783, 'CTTAG': 51299, 'CTTTA': 58644, 'CTTTT': 153390, 'CTTTC': 159132, 'CTTTG': 204142, 'CTTCA': 269549, 'CTTCT': 252313, 'CTTCC': 376392, 'CTTCG': 141706, 'CTTGA': 109325, 'CTTGT': 99941, 'CTTGC': 123886, 'CTTGG': 157440, 'CTCAA': 193954, 'CTCAT': 158201, 'CTCAC': 200735, 'CTCAG': 263727, 'CTCTA': 83576, 'CTCTT': 169484, 'CTCTC': 218844, 'CTCTG': 297563, 'CTCCA': 341404, 'CTCCT': 340453, 'CTCCC': 404801, 'CTCCG': 204631, 'CTCGA': 64613, 'CTCGT': 61248, 'CTCGC': 114084, 'CTCGG': 187175, 'CTGAA': 246735, 'CTGAT': 147020, 'CTGAC': 205211, 'CTGAG': 305260, 'CTGTA': 104857, 'CTGTT': 142146, 'CTGTC': 216774, 'CTGTG': 323692, 'CTGCA': 353060, 'CTGCT': 410493, 'CTGCC': 528709, 'CTGCG': 247289, 'CTGGA': 509499, 'CTGGT': 277992, 'CTGGC': 464361, 'CTGGG': 499187, 'CCAAA': 178976, 'CCAAT': 84743, 'CCAAC': 231482, 'CCAAG': 412947, 'CCATA': 62129, 'CCATT': 119196, 'CCATC': 324499, 'CCATG': 292713, 'CCACA': 254698, 'CCACT': 195583, 'CCACC': 475561, 'CCACG': 208649, 'CCAGA': 369327, 'CCAGT': 222068, 'CCAGC': 582482, 'CCAGG': 492542, 'CCTAA': 62938, 'CCTAT': 92925, 'CCTAC': 195851, 'CCTAG': 68047, 'CCTTA': 65747, 'CCTTT': 162012, 'CCTTC': 343639, 'CCTTG': 172368, 'CCTCA': 323320, 'CCTCT': 276324, 'CCTCC': 501803, 'CCTCG': 179035, 'CCTGA': 309194, 'CCTGT': 237921, 'CCTGC': 572447, 'CCTGG': 707861, 'CCCAA': 246810, 'CCCAT': 188549, 'CCCAC': 358348, 'CCCAG': 595186, 'CCCTA': 125163, 'CCCTT': 215365, 'CCCTC': 360865, 'CCCTG': 556845, 'CCCCA': 493017, 'CCCCT': 396584, 'CCCCC': 593698, 'CCCCG': 342329, 'CCCGA': 193637, 'CCCGT': 124997, 'CCCGC': 307255, 'CCCGG': 352238, 'CCGAA': 77989, 'CCGAT': 54995, 'CCGAC': 163985, 'CCGAG': 276971, 'CCGTA': 35896, 'CCGTT': 49430, 'CCGTC': 141349, 'CCGTG': 191926, 'CCGCA': 214186, 'CCGCT': 207602, 'CCGCC': 448097, 'CCGCG': 206508, 'CCGGA': 211719, 'CCGGT': 79077, 'CCGGC': 318516, 'CCGGG': 356087, 'CGAAA': 40449, 'CGAAT': 39665, 'CGAAC': 49436, 'CGAAG': 86472, 'CGATA': 21426, 'CGATT': 38314, 'CGATC': 38741, 'CGATG': 94835, 'CGACA': 118886, 'CGACT': 85778, 'CGACC': 147808, 'CGACG': 112506, 'CGAGA': 185632, 'CGAGT': 112609, 'CGAGC': 193753, 'CGAGG': 297104, 'CGTAA': 22973, 'CGTAT': 17304, 'CGTAC': 37476, 'CGTAG': 25226, 'CGTTA': 12333, 'CGTTT': 40445, 'CGTTC': 47856, 'CGTTG': 46639, 'CGTCA': 118687, 'CGTCT': 112262, 'CGTCC': 138446, 'CGTCG': 54929, 'CGTGA': 102742, 'CGTGT': 93681, 'CGTGC': 174943, 'CGTGG': 259735, 'CGCAA': 130567, 'CGCAT': 100009, 'CGCAC': 113311, 'CGCAG': 169437, 'CGCTA': 71862, 'CGCTT': 97634, 'CGCTC': 142601, 'CGCTG': 279036, 'CGCCA': 256719, 'CGCCT': 241119, 'CGCCC': 298830, 'CGCCG': 247569, 'CGCGA': 84243, 'CGCGT': 65154, 'CGCGC': 154778, 'CGCGG': 181968, 'CGGAA': 149119, 'CGGAT': 75334, 'CGGAC': 144848, 'CGGAG': 277055, 'CGGTA': 29433, 'CGGTT': 47291, 'CGGTC': 78730, 'CGGTG': 129483, 'CGGCA': 212880, 'CGGCT': 201629, 'CGGCC': 386868, 'CGGCG': 259003, 'CGGGA': 225191, 'CGGGT': 103235, 'CGGGC': 274283, 'CGGGG': 275217, 'GAAAA': 177046, 'GAAAT': 110188, 'GAAAC': 154235, 'GAAAG': 183964, 'GAATA': 56769, 'GAATT': 83389, 'GAATC': 76410, 'GAATG': 125883, 'GAACA': 172665, 'GAACT': 138731, 'GAACC': 172914, 'GAACG': 101757, 'GAAGA': 452209, 'GAAGT': 174708, 'GAAGC': 334555, 'GAAGG': 340632, 'GATAA': 53026, 'GATAT': 47249, 'GATAC': 45614, 'GATAG': 37785, 'GATTA': 47038, 'GATTT': 92734, 'GATTC': 97392, 'GATTG': 85625, 'GATCA': 143672, 'GATCT': 115005, 'GATCC': 195240, 'GATCG': 77238, 'GATGA': 287672, 'GATGT': 128596, 'GATGC': 168284, 'GATGG': 235398, 'GACAA': 208004, 'GACAT': 169839, 'GACAC': 153736, 'GACAG': 230691, 'GACTA': 67283, 'GACTT': 129044, 'GACTC': 168921, 'GACTG': 169654, 'GACCA': 301867, 'GACCT': 240365, 'GACCC': 305272, 'GACCG': 140395, 'GACGA': 137260, 'GACGT': 93238, 'GACGC': 160007, 'GACGG': 170459, 'GAGAA': 362370, 'GAGAT': 199092, 'GAGAC': 225362, 'GAGAG': 257286, 'GAGTA': 85770, 'GAGTT': 133653, 'GAGTC': 147808, 'GAGTG': 188785, 'GAGCA': 268971, 'GAGCT': 296007, 'GAGCC': 372117, 'GAGCG': 222105, 'GAGGA': 560100, 'GAGGT': 216178, 'GAGGC': 395762, 'GAGGG': 293607, 'GTAAA': 57414, 'GTAAT': 40721, 'GTAAC': 43631, 'GTAAG': 36076, 'GTATA': 24107, 'GTATT': 56051, 'GTATC': 45056, 'GTATG': 80264, 'GTACA': 102450, 'GTACT': 68621, 'GTACC': 117704, 'GTACG': 50109, 'GTAGA': 59802, 'GTAGT': 35203, 'GTAGC': 51344, 'GTAGG': 39562, 'GTTAA': 42023, 'GTTAT': 34390, 'GTTAC': 44045, 'GTTAG': 35145, 'GTTTA': 47038, 'GTTTT': 109452, 'GTTTC': 100448, 'GTTTG': 149973, 'GTTCA': 134675, 'GTTCT': 141273, 'GTTCC': 151461, 'GTTCG': 74264, 'GTTGA': 66645, 'GTTGT': 79056, 'GTTGC': 81452, 'GTTGG': 113037, 'GTCAA': 121893, 'GTCAT': 115981, 'GTCAC': 139709, 'GTCAG': 156271, 'GTCTA': 61925, 'GTCTT': 115305, 'GTCTC': 159349, 'GTCTG': 182707, 'GTCCA': 187769, 'GTCCT': 206772, 'GTCCC': 249840, 'GTCCG': 105059, 'GTCGA': 50015, 'GTCGT': 68347, 'GTCGC': 78839, 'GTCGG': 96533, 'GTGAA': 202929, 'GTGAT': 106218, 'GTGAC': 182824, 'GTGAG': 170982, 'GTGTA': 61527, 'GTGTT': 123552, 'GTGTC': 135476, 'GTGTG': 215620, 'GTGCA': 186178, 'GTGCT': 209951, 'GTGCC': 252738, 'GTGCG': 134173, 'GTGGA': 335837, 'GTGGT': 222003, 'GTGGC': 342467, 'GTGGG': 295653, 'GCAAA': 134332, 'GCAAT': 70376, 'GCAAC': 129317, 'GCAAG': 284734, 'GCATA': 30021, 'GCATT': 88299, 'GCATC': 193309, 'GCATG': 155147, 'GCACA': 156337, 'GCACT': 136705, 'GCACC': 276308, 'GCACG': 135179, 'GCAGA': 287766, 'GCAGT': 187460, 'GCAGC': 514633, 'GCAGG': 354016, 'GCTAA': 53226, 'GCTAT': 78912, 'GCTAC': 154688, 'GCTAG': 46101, 'GCTTA': 42363, 'GCTTT': 134901, 'GCTTC': 247974, 'GCTTG': 110996, 'GCTCA': 225088, 'GCTCT': 195845, 'GCTCC': 321232, 'GCTCG': 97783, 'GCTGA': 309632, 'GCTGT': 249609, 'GCTGC': 544286, 'GCTGG': 559347, 'GCCAA': 262803, 'GCCAT': 280807, 'GCCAC': 343354, 'GCCAG': 411593, 'GCCTA': 116648, 'GCCTT': 227662, 'GCCTC': 382356, 'GCCTG': 477685, 'GCCCA': 394407, 'GCCCT': 399466, 'GCCCC': 552780, 'GCCCG': 331374, 'GCCGA': 196641, 'GCCGT': 136275, 'GCCGC': 397579, 'GCCGG': 294837, 'GCGAA': 50955, 'GCGAT': 52399, 'GCGAC': 106878, 'GCGAG': 191328, 'GCGTA': 20865, 'GCGTT': 41916, 'GCGTC': 119958, 'GCGTG': 170279, 'GCGCA': 154536, 'GCGCT': 172314, 'GCGCC': 284642, 'GCGCG': 153318, 'GCGGA': 205264, 'GCGGT': 86660, 'GCGGC': 419501, 'GCGGG': 260432, 'GGAAA': 177615, 'GGAAT': 100200, 'GGAAC': 159623, 'GGAAG': 350046, 'GGATA': 52905, 'GGATT': 98458, 'GGATC': 123685, 'GGATG': 242676, 'GGACA': 289655, 'GGACT': 199828, 'GGACC': 348311, 'GGACG': 200152, 'GGAGA': 437702, 'GGAGT': 192645, 'GGAGC': 460996, 'GGAGG': 587891, 'GGTAA': 40488, 'GGTAT': 47651, 'GGTAC': 72907, 'GGTAG': 51951, 'GGTTA': 35249, 'GGTTT': 100600, 'GGTTC': 120265, 'GGTTG': 93966, 'GGTCA': 163806, 'GGTCT': 155962, 'GGTCC': 206414, 'GGTCG': 104734, 'GGTGA': 204898, 'GGTGT': 166637, 'GGTGC': 226892, 'GGTGG': 414961, 'GGCAA': 191644, 'GGCAT': 157904, 'GGCAC': 212025, 'GGCAG': 373322, 'GGCTA': 114882, 'GGCTT': 178044, 'GGCTC': 250884, 'GGCTG': 439593, 'GGCCA': 453966, 'GGCCT': 382463, 'GGCCC': 583550, 'GGCCG': 381930, 'GGCGA': 133392, 'GGCGT': 128403, 'GGCGC': 259947, 'GGCGG': 359626, 'GGGAA': 201539, 'GGGAT': 122035, 'GGGAC': 273889, 'GGGAG': 331891, 'GGGTA': 51517, 'GGGTT': 100112, 'GGGTC': 189990, 'GGGTG': 222106, 'GGGCA': 281034, 'GGGCT': 284655, 'GGGCC': 520896, 'GGGCG': 244999, 'GGGGA': 274831, 'GGGGT': 156351, 'GGGGC': 390548, 'GGGGG': 276093}, 'overrepresented_sequences': {}} {'passed_filter_reads': 6242804, 'low_quality_reads': 374378, 'too_many_N_reads': 758, 'too_short_reads': 78, 'too_long_reads': 0} fastp -i /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045021_1.fastq.gz -I /home/ubuntu/workspace/rnaseq/team_exercise/untrimmed/SRR10045021_2.fastq.gz -o /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045021.trimmed_1.fastq.gz -O /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045021.trimmed_2.fastq.gz -l 25 --adapter_fasta /home/ubuntu/workspace/rnaseq/team_exercise/references/illumina_multiplex.fa --trim_tail1 5 --trim_tail2 5 --json /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045021.fastp.json --html /home/ubuntu/workspace/rnaseq/team_exercise/trimmed/SRR10045021.fastp.html